Polaroid Type 55/665 negative racks and tanks can be purchased from
Graphic Center P.O. Box 818 Ventura, CA 93002 (805) 383-6864
The PN-10 Clearing Tank includes a 4 quart plastic tank with lid and handle and a 12 slot negative rack. It comes with 1 lb of sodium sulfite too. Cost is $35.95 in August 1994 catalog.
At 09:45 AM 9/29/95 -0400, you wrote: } Dear Bob: We have also spent an enormous amount of time searching for the white buckets for the Polaroid 4"x5" negs. Polaroid } informed us that they do not manufacture them. There are many Lucite racks available for this purpose but if you work in the dark } (like me) the racks are very difficult to use. It's like trying to thread a needle in the dark. Should you find the white buckets please } let me know. Thanks. Sincerely, Charlie Murphy } } Owen P. Mills Michigan Technological University Metallurgical & Materials Engineering Rm 512 MME Building Houghton, MI 49931 906-487-2002 906-487-2934 FAX opmills-at-mtu.edu
I am looking for information about applications of electrochemical AFM or STM cells. If anyone knows of any papers or has used electrochemical SPM for their applications, I would appreciate your response. Thanks, Melanie Behrens
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Yes, I would have said that myself having found magnification values pretty meaningless when attempting to put a size on objects, but this dialogue has made me realise why there is no totally correct solution....
The micron marker concept only works if the depth of field is negligible. That is the case with thin specimens in TEM, and almost the case with flat specimens normal to the beam in SEM, and for optical microscopes with very small depth of field but for rough or inclined specimens in SEM or for photographs of large objects, the size (micron) marker would have to be adjusted to match the depth of the object behind the viewing port - not very easy to do unless the object is viewed in true stereo.
Anyone got any tidy way round this problem of definition?
Peter Statham Oxford Instruments Microanalysis Group } } Why bother with magnification at all. I always simply } place a "micron" (or nanometer etc..) marker on the image. } In this way the image is always calibrated regardless of } what anyone else does to the print after you give it to them. } } Nestor } Your Friendly Neighborhood SysOp }
----------------------------------------------------------------- Please reply to this e-mail with the name of the person you wish to receive it on the subject line (e.g. "FAO Janet Smythe/..subject.."), as this is a shared e-mail address.
Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za} "Simon Watkins Ph.D." {swatkins-at-pitt.edu}
Good day Simon Your problem appears to be due to the colour temperature of the illumination not matching that for which the film is balanced (3200K or 3400K) resulting in reciprocity failure. This is why the fault is not seen on digital images. Using daylight film and the microscope's blue filter, we consistently got a reddish-brown background in our prints. (Using the recommended 9V setting instead of 12V made matters 10x worse!) The answer to your problem lies in establishing exactly what filters need to be inserted in the light path to correct the colour temperature to suit the (tungsten) film. These will be in the 82 series, where each filter increases the temperature of the illumination by 100K. Make sure to record whether the illumination diffuser is in or out when you photograph using the different filters since it affects the temperature of the illumination. I would like to refer you to a Kodak publication, "Photography through the microscope" by John Delly where you will find all the information you require.
In a perverse kind of way, I'm glad that we are not the only ones with this kind of problem!
Hope this helps you.
James Wesley-Smith Electron Microscope Unit George Campbell Building University of Natal Durban, South Africa
We have this problem constantly with users. They collect their images with the BioRad confocal at different zoom settings or with Newvicon or CCD video cameras and then ask for magnification. So I go into an explanation of why magnification is not resolution. To have this problem solved, I recommend they write in the figure legend what optics and what electonic devices were used. I also recommedn adding scale bars. The readers can immediately see the magnification and can also judge the resolution if this is critical. Practically, this is a fair trade off. If for phase contrast ot fluorescence we say we used a 100X N.A. 1.4 objective with t-Max 100 film or with the BioRad confocal, you have an idea of the resolution. For critical applications, such as microtubule motility assays, the methods would be more detailed. A glance at the scale bar tells you magnification. Considering that journals print with half-tone, this, practically, solves the problem. Similarly, people zoom or shrink images on their computers without regard for what is happening to the resolution of each pixel and the problem of magnification vs. resolution is far worse than with film. -Michael C.
Yes, I too have used the lucite racks which due to the inherent curl to the polaroid negs can be a real pain in the neck to use. Additionally since the polaroid negs are thiner than normal Kodak/Ilford/etc. type plate films, combined with their surface area the polaroid negs then to pop out of the lucite rack slots very easily. An alternative that I have found works very well is to use Kodak Film Hanger No. 6, which are stainless steel and have two clips which grab on to the edges of the film. These holders work for sheet films from 3 inches wide to 6 inches wide with out difficulty, very easily. The only problem is that the holders are expensive at $12.50 each! But most Kodak vendors can order these from Kodak and provide them. The Film hangers will fit into any 4x5 neg developing tanks or tupperware containers.
I know this is not allowed on the listserver but I am getting very frustarted with job hunting
I am a grad student completing my M.A. in biology ac CSUF and am in search or a EM technician position. I am listed with the MSA placement office and have appled for every position they have had open and I have sent out over 100 resumes. As of yet I have jeard from 8% of then all with unfavorable results. I have found many postdoc positions that I can do but they will not hrie a biologist with a M.A. I have looked in other cities newspapers, on he internet and what ever other resource I can find.
I did not think it was this difficult to find a position. If anybody knows of any fulltime permanent positions could you let me know Please? If anyone would like to see a CV I can sent it to you
I have the following qualifications:
As part of my thesis work which is cellular and morphological, I am investigating the morphological and biochemical characteristics of the extracellular matrix of eggs. This work has revealed that the egg coats swell when released into seawater and that this extracellular matrix is composed of many types of sugar moieties , and ECM proteins as revealed by lectin and antibody immunofluorescence labeling.
I have these and other qualifications:
Completed a course in Transmission Electron Microscopy with an "A".
Completed lecture and lab course in Microbiology with "A" and "B" respectively.
Utilized Electron Microscopy (TEM and SEM) as part of my Master's Thesis research.
Knowledge of basic biochemistry skills (SDS PAGE, native gels, westerns, and column chromatography).
Taught undergraduate laboratory class in mammalian physiology.
Immunolabeling techniques for light microscopy and electron microscopy.
Proficient in Photomicrography (Light and EM level) and darkroom skills.
Proficient with Word perfect, Quattro Pro, Sigmaplot, Sigmastat, Freehand, Photoshop.
Proficient with computer vbased image analysis with a Compix (c-imaging) image analysis system and CSPI (Scanalytics) deconvolution software.
I have also learned the following techniques: immunolabeling (light microscopy and EM), TEM, SEM, freeze-fracture TEM, sectioning (ultramicrotome), EM technique of room temp. molecular shadowing , spectrofluorimetry, spectrophotometry, darkroom techniques and computer based image analysis.
The results of the first part on my thesis research were published in Molec. Biol.. Cell. 5, 94a. and Micros. Res. Tech., 29, (6) 495. (see resume). These results were also presented at the American Society for Cell Biology meeting in 1994, and the California State University EM symposium in 1994.
I would appreciate an opportunity to discuss my academic and job experience with you and to provide you with details not listed on the enclosed resume. I am in the process writing my thesis. I can be reached during the day at (602) 832-2885. I am highly motivated and look forward to hearing from you soon.
"Charles J. Butterick" {emccjb-at-ttuhsc.edu} , microscopy-at-aaem.amc.anl.gov
I routinely advise users of my electro optical equipment to record an image of a stage micrometer along with their images. They also record the settings of the microscope on the Universal Imaging set up . That is keeping dual records. It is so much easier than back tracking. Nina Allen.
On Mon, 2 Oct 1995, Michael Cammer wrote:
} We have this problem constantly with users. They collect their images } with the BioRad confocal at different zoom settings or with Newvicon or } CCD video cameras and then ask for magnification. So I go into an } explanation of why magnification is not resolution. To have this } problem solved, I recommend they write in the figure legend what optics } and what electonic devices were used. I also recommedn adding scale } bars. The readers can immediately see the magnification and can also } judge the resolution if this is critical. Practically, this is a fair } trade off. If for phase contrast ot fluorescence we say we used a 100X N.A. } 1.4 objective with t-Max 100 film or with the BioRad confocal, you have } an idea of the resolution. For critical applications, such as } microtubule motility assays, the methods would be more detailed. A } glance at the scale bar tells you magnification. Considering that } journals print with half-tone, this, practically, solves the problem. } Similarly, people zoom or shrink images on their computers without regard } for what is happening to the resolution of each pixel and the problem of } magnification vs. resolution is far worse than with film. -Michael C. }
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One thing that you might try is to tell your people to make photographs of a stage micrometer at the same equipment settings and to manipulate it the same way they do their other micrographs. Then they would have a magnification calibration in hand, that may be good only for the work they did that day. But it would be a simple matter to calculate the actual magnification of their finished work. After all the only mag value that is important is the ratio of their final image, be it of their cells, tissue, etc., and the specimen on their microscope stage, be it tissue or micrometer.
At 11:34 AM 10/2/95 -0400, you wrote: } We have this problem constantly with users. They collect their images } with the BioRad confocal at different zoom settings or with Newvicon or } CCD video cameras and then ask for magnification. So I go into an } explanation of why magnification is not resolution. To have this } problem solved, I recommend they write in the figure legend what optics } and what electonic devices were used. I also recommedn adding scale } bars. The readers can immediately see the magnification and can also } judge the resolution if this is critical. Practically, this is a fair } trade off. If for phase contrast ot fluorescence we say we used a 100X N.A. } 1.4 objective with t-Max 100 film or with the BioRad confocal, you have } an idea of the resolution. For critical applications, such as } microtubule motility assays, the methods would be more detailed. A } glance at the scale bar tells you magnification. Considering that } journals print with half-tone, this, practically, solves the problem. } Similarly, people zoom or shrink images on their computers without regard } for what is happening to the resolution of each pixel and the problem of } magnification vs. resolution is far worse than with film. -Michael C. } }
Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy Department of Pathology, Rm.286-A Baylor College of Medicine One Baylor Plaza Houston, Texas 77030 U.S.A. tel.: (713)798-4658 FAX: (713)798-3945 joiner-at-bcm.tmc.edu Compuserve: 71555,1206
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Probably the best solution is to add size-calibrated objects to the specimen. Often SEM people will add latex beads of a calibrated size to their samples. Of course this is not always practical. I often use naturally occuring internal standards that show up in other peoples micrographs. Collagen, microtubules, and other organelles of known size can be used in this way, keeping in mind that specimen handeling can cause shrinkage. On occasion I have caught errors in stated magnifications in this manner.
At 04:05 PM 9/29/95, you wrote: } Yes, I would have said that myself having found magnification values } pretty meaningless when attempting to put a size on objects, but } this dialogue has made me realise why there is no totally correct } solution.... } } The micron marker concept only works if the depth of field is } negligible. That is the case with thin specimens in TEM, and almost } the case with flat specimens normal to the beam in SEM, and for } optical microscopes with very small depth of field but for rough or } inclined specimens in SEM or for photographs of large objects, the } size (micron) marker would have to be adjusted to match the depth of } the object behind the viewing port - not very easy to do unless the } object is viewed in true stereo. } } Anyone got any tidy way round this problem of definition? } } Peter Statham } Oxford Instruments Microanalysis Group } } } } Why bother with magnification at all. I always simply } } place a "micron" (or nanometer etc..) marker on the image. } } In this way the image is always calibrated regardless of } } what anyone else does to the print after you give it to them. } } } } Nestor } } Your Friendly Neighborhood SysOp } } } } ----------------------------------------------------------------- } Please reply to this e-mail with the name of the person you } wish to receive it on the subject line } (e.g. "FAO Janet Smythe/..subject.."), } as this is a shared e-mail address. } } } } }
Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy Department of Pathology, Rm.286-A Baylor College of Medicine One Baylor Plaza Houston, Texas 77030 U.S.A. tel.: (713)798-4658 FAX: (713)798-3945 joiner-at-bcm.tmc.edu Compuserve: 71555,1206
I have a very good and folwing coverletter. To date I have had 7 professors and other faculty read it for me. They cannot se anything I need to change with it. They all like how it flows and reads.
} } } As a tangent to the current thread on magnification and resolution, our } } } photographer today inquired about the difference between a macrograph } } } (e.g., a photograph made using a macro lens) and a micrograph. Anyone } } } want to jump in on this? } } } } } } James Martin } } } } } } } } ************ } } OK, I'll give it a try. I am going to say that a camera with a macro } } lens....IF it is set up to record images larger than the photographed } } subject (ie. 1:1+).....is equivalent to a microscope with a film-back. } } Therefore your photographer's "macrograph" is the same as a micrograph. I } } think that macrograph may be a misnomer, or at the most, jargon. Many } } photographers use macrography to mean recording images between what a } } "standard" camera lens can do and what a close-up lens can do up to an image } } ratio of 1:1, ie. not magnifying.
Macro photography is that which can be done conveniently with a single lens and bellows.
Special macro-lenses are sold for this purpose and they work in the range up to about 10x. This implies a lens-to-film distance about 10 times lens-to-subject distance and, with a 25 mm lens, 10x about all you want to try. In addition, such macrophotograpy setups are quite sensitive to vibration and it is hard to arrange the lighting to provide an image bright enough to focus the image on the ground glass. Finally, like all optics, macro setups are limited by diffraction and aberrations and microscope objectives can be better corrected because they only operate at a single magnification. (while the macroscope magnification depends on the bellows extention)
Jim Pawley
***************************************** Prof. James B Pawley, Ph. 608-263-3147 Room 1235, Engineering Research Building, FAX 608-265-5315 1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
Whereas Joiner Cartwright stated that he feels macrography is a misnomer and should not be used for anything over 1x I tend to disagree though it maybe just a matter of semantics.
For the past 15 years I have been using the definitions provided by Canon Inc.: General Photography: 0.1x and lower Close-up Photography: 0.1x - 1x Photomacrography: 1x - 10x (- 20x) Photomicrography: } 10x
I suppose these ranges are somewhat arbitrary but they do have specific equipment required for each range:
General Photo.: normally-mounted standard lens alone. Close-up: close-up lenses (macro lenses) or extension tubes Macrography: main accessory is a bellows, often revese mounted lenses (note: this is is acomplished with a single lens system located at a distance from the film plane, there is no second lens system.) Micrography: replacment of 'normal' camera lenses with a microscopic lens system, i.e. a compound light microscope. (Two separated lens systems, the objectives and the projector/ocular)
{Granted I am not a optics specialist.}
Now this definition does present a small problem when dealing with the differences between a compound light microscope [10 - 1,200x] and a macroscope - a.k.a. Disecting scope (which is a really awful name!) [4x - 250x]. All the journals I have dealt with insisted that the term microscope be used and macroscope not be used, but it think that this is in accurate as the two types of scopes are very different and when I am reading a scientific publication I do like to know what equipment is being used. The work depth of field at 100x in a macroscope is much different than 100x in a microscope, eh?
But can we as a microscopy (including macroscopy?) community come to some sort of a consensus on this?
I know it sounds harsh, but Eric Rosen broke one of our cardinal rules. I have sent him several notes off-line and then deleted him from the subscription list. I have said no to many people who have politely asked about posting resume's some of which I know personally. I have to keep a firm position here. No offense meant to anyone looking for a job.
Nestor....
P.S. I also pointed him to several places where he could post and/or send resume's.
The following response to my recent query on bounced error messages, requires an on-line answer.
} } Dear Mr. Haug: } } Please be advised that the administrator of the Microscopy list server does this on a voluntary, unpaid basis. Furthermore, the administrator, Dr. Zaluzec, is a highly respected scientist, travels frequently, and often can not respond on a timely basis to each request for help. Your incivility of inferring that the administrator is inhuman is insensitivive and unacceptable for this type of a forum. I also volunteer my time as an administrator of a technical list server, and I can tell you from first hand experience that it is a thankless task, a service that I render to contribute to the sciences that I practice. I believe that you owe Dr. Zaluzec, as well as the 2000 memebers of this list server, an apology. } } Respectfully submitted, }
The paragraph which provoked this response was
} } } } A few days ago, I reported this to Postmaster-at-aaem.amc.anl.gov, } } hoping that she would be a human being, able to point out what is } } wrong, which she is probably not since there has been no reply } } in point. } }
The above interpretation was !!not my intention. Sorry if clumsy writing gave the impression of ingratitude towards Nestor and others who in their spare time are building new communication channels for the scientific community.
I fully appreciate the amount of work it takes to raise and support a large list, checking the message-flow, acquiring and implementing new software versions, dealing with people responsible for the host machine, budgetary issues, - - - .
University of Oslo Institute of basic medical sciences Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no Box 1105 Blindern Phone : +47 22 85 12 67 N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
I have made and used for 20 years lucite holders for polaroid film. I have 2 holders 18.5 *11.3 * 11 cm high with handles 18 cm high with 11 slots 1.3 * 10 * 11 cm high. These slots are designed to hold the film emulsion side concave inward so the solution acts on the emulsion and it does not curl the other way. In practice, they still curl back but it does not hurt. I soak in water 1 minute to 8 hours (ie at least a short soak but all day is ok) then 18 % sodium sulfite as advised by polaroid then 5 minutes (to several hours but not over night) in running tap water, rinse in Deionized water and transfer to second racks (below). Occasionally, as the sulfite gets old I have to squeegee off a little jelly clinging to the surface. These racks are designed to fit standard 1 gallon stainless steel tanks. The second rack, which I have 8 but could sometimes use 12 or more, are copied after some commercial racks with curling slots but my slots are further apart (the commercials ones went out of business 15 years ago). These drying racks are 23 * 11.5 * 15.5 cm high and have 10 curving slots down the side of the long axis to hold 10 films while drying. 11.5 cm below the top is a lucite bar with straight slots to hold the bottom of each film. The curving slots are spaced 2 cm apart, are 12.5 cm high, 0.3 cm deep and 0.2 cm wide. The curve is bell shaped, flat at top and bottom and the curve is displaced 1.2 cm 'sideways' in the middle ( about 8 cm of the slot is humped sideways. These slots are formed from 0.2 cm thick lucite, cut with the curve both sides and glued (acetone) to the side plates of the lucite rack. Some care is needed to avoid curling of the film or the negative become stuck together ( a long soak in hot water will enable almost all such stuck negatives to be rescued, but prevention is easier. These racks do not fit a tank and are never used for soaking, only drying. I would be willing to post details, if enough people are interested.
Sorry about the length of this.
Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis SEM/Morphometrics lab Marine & Coastal Sciences Rutgers University 908 932 8959 ext 225 Pooley-at-ahab.rutgers.edu
On Mon, 2 Oct 1995, Richard E. Edelmann wrote:
} Yes, I too have used the lucite racks which due to the inherent } curl to the polaroid negs can be a real pain in the neck to use. } Additionally since the polaroid negs are thiner than normal } Kodak/Ilford/etc. type plate films, combined with their surface area } the polaroid negs then to pop out of the lucite rack slots very } easily. An alternative that I have found works very well is to use } Kodak Film Hanger No. 6, which are stainless steel and have two clips } which grab on to the edges of the film. These holders work for sheet } films from 3 inches wide to 6 inches wide with out difficulty, very } easily. The only problem is that the holders are expensive at $12.50 } each! But most Kodak vendors can order these from Kodak and provide } them. The Film hangers will fit into any 4x5 neg developing tanks or } tupperware containers. } } }
Usually images taken with the TEM are focused on a specimen's high point at ~0-100A underfocus so that the specimen beneath is underfocused. In a high resolution instrument with a focal length of 2.0 mm Scherzer focus occurs at 450-750A underfocus and the useful depth of field is not much larger than this value. In an ordinary TEM with a focal length of 5 mm, Schertzer focus occurs at about 1350-1400 A with a useable depth of field also extending to a larger value. If you calculate depth of field for the 5 mm focal length instrument, it is ~2650A however about a third of this depth may be too underfocused to be useful. My question is how much does the magnification change for these underfocus values? My impression is that freeze dried objects of known size, 50-250A, are approximately the same size over this range of focus values and the change in magnification is small! This observation suggests that a magnification bar on a TEM micrograph is a good estimate of the size of objects in an enlarged micrograph!
George C. Ruben Dept. Biological Sciences Dartmouth College Hanover, NH 03755
It is not too late to register for the Great Lakes Microscopy Conference to be held at the Toledo Hilton Dana Center in Toledo, Ohio, October 12-14. Talks are on various areas of microscopy-Biology, Materials Science, Pathology, Forensic Science etc. Student Registration $20.00 Non-student $30.00. Contact C.Heckman at 419-372-8218 e-mail: heckman-at-bgnet.bgsu.edu for further details.
"...inquired about the difference between a macrograph (e.g., a photograph made using a macro lens) and a micrograph."
macro-: in Greek comp., long, large, thick, great; micro-: in Greek comp., little, small, minute;
Stereo-microscopes, hand held glass lenses, any macro-setup and SEMs are used to increase the surface morphology of the specimen by reflected light . SEMs form SE or X-ray image of the "bulk" sample's surface, regardless it's fractured or not. These are macro images.
When the image is formed by using trasmitted light or e-beam, the "micro" internal image of the section is formed. (LM, TEM...) Sections are produced by "micro"tomes or ultra"micro"tomes to expose the internal fine stuctures of the specimen. These are micro images.
I hope it helps.
Laszlo J. Veto Tel: 604-494-7711 Electron Microscopist Fax: 604-494-0755 Research Centre e-Mail Veto-at-bcrssu.agr.ca Summerland, B.C. Canada V0H 1Z0
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Does anybody know who services LKB ultramicrotomes in the Houston area? I just inherited an LKB Nova and it needs some attention.
Thanks
Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy Department of Pathology, Rm.286-A Baylor College of Medicine One Baylor Plaza Houston, Texas 77030 U.S.A. tel.: (713)798-4658 FAX: (713)798-3945 joiner-at-bcm.tmc.edu Compuserve: 71555,1206
Hi Eric, Just to let you know, it took me almost 2 years to find a new job. Hang in there and they will come. The best way was to knock on doors, very few technicial postions are advertised outside the institution. A face to face meeting is much better than any CV.
Best of Luck, Ed Calomeni Dept Pathology Medical College of Ohio Toledo, OH 43699 emlab-at-opus.mco.edu
I am having problems sectioning tissue blocks that are 500=B5m thick, I woul= d like to have 10; 50=B5m sections in the end. Using a vibratome I have tried mounting the tissue onto 15% gelatin, the tissue seems secure but then falls off the chuck before I'm finished cutting. I can't use superglue, it messes up the tissue more than I do. I need to use the vibratome - this is essential. I tried using the cryostat and have gotten good sections, however I was not able to stain the sections. Can anyone give me suggestions on how to secure a 500=B5m slice onto a chuck so that the tissue in fairly flat and won't fall off?
Ted Bell, bell-at-medcolpa.edu Medical College of Pennsylvania and Hahnemann University Neurobiology and Anatomy http://ussanatomy.medcolpa.edu/FaberWeb/confocal.html
I always thought "macro"-whatever referred to an object which was visible to the unaided eye, whereas "micro"-whatever was discernible only with the aid of some kind of magnifying device, such as a microscope.
Certainly objects can be discernible, yet still small, and require special setups for photography. Hence, macro lenses.
Carl
Carl Henderson Electron Microbeam Analysis Laboratory University of Michigan 2005 C.C. Little Bldg. Ann Arbor, MI 48109-1063 USA -------------------------------- (313) 936-1550 (voice) (313) 763-4690 (FAX) chender-at-umich.edu (e-mail) --------------------------------
X-Sender: pathgs-at-kokako.wnmeds.ac.nz X-Mailer: Windows Eudora Version 1.4.3 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy-at-aaem.amc.anl.gov
Tiring of the struggle to maintain and build up a suitable collection of mineral standards for the calibration of the elderly electron microprobe which I run/maintain/love/hate, I am near to the point of ordering from SPI their 53-mineral mount cat # 02753-AB.
Has anyone out there used this product for standardisation for quantitative geological analytical work? Found it to be good? Should I buy it?
Maybe email me direct to avoid commercial sensitivities, although I don't think that Charles Garber would shy from open debate, would you, Charles?
thanks
Ritchie Sims phone: 61 9 3737599 ext 7713 Department of Geology fax: 61 9 3737435 University of Auckland Private Bag 92019 Auckland New Zealand
} Whereas Joiner Cartwright stated that he feels macrography is a } misnomer and should not be used for anything over 1x I tend to } disagree though it maybe just a matter of semantics. } } For the past 15 years I have been using the definitions provided by } Canon Inc.: } General Photography: 0.1x and lower } Close-up Photography: 0.1x - 1x } Photomacrography: 1x - 10x (- 20x) } Photomicrography: } 10x } } I suppose these ranges are somewhat arbitrary but they do have } specific equipment required for each range: } } General Photo.: normally-mounted standard lens alone. } Close-up: close-up lenses (macro lenses) or extension tubes } Macrography: main accessory is a bellows, often revese mounted } lenses (note: this is is acomplished with a single lens system } located at a distance from the film plane, there is no second lens } system.) } Micrography: replacment of 'normal' camera lenses with a } microscopic lens system, i.e. a compound light microscope. (Two } separated lens systems, the objectives and the projector/ocular) } } {Granted I am not a optics specialist.} } } } Now this definition does present a small problem when dealing with } the differences between a compound light microscope [10 - 1,200x] and } a macroscope - a.k.a. Disecting scope (which is a really awful name!) } [4x - 250x]. All the journals I have dealt with insisted that the } term microscope be used and macroscope not be used, but it think that } this is in accurate as the two types of scopes are very different and } when I am reading a scientific publication I do like to know what } equipment is being used. The work depth of field at 100x in a } macroscope is much different than 100x in a microscope, eh? } } But can we as a microscopy (including macroscopy?) community come to } some sort of a consensus on this?
Yes, it would indeed be good if microscopists could agree on some sort of consesus on the terminology. My opinion on the matter is that the Canon definitions can well be used as a standard (even though the distinction between macro- and micrography creates some difficulties with different microscope systems, see below). The only question is how can "we" get everyone (more or less) to stick to a standard whether it is this one or something else that "we" can agree on?!
(This is actually quite an interesting problem in itself: can a discussion group like this one come to a consesus on a certain matter, and how should that then be implemented? In a conference or society meeting with chairman etc. there can be taken a "democratic" decision, but how do we do here?)
Back to microscopes and the distinction between different types: A compound microscope consists of two sets of optics (objective and ocular) that form the image and illumination which can be incident, transmitted, or sideways. It doesn't really matter wether you are looking at the surface of a specimen or through a section of the specimen. (Leitz used to make an excellent set of optics called the "Ultropak" system with lenses up to 40x that could be immersed in water solutions and used for very close up studies of dissected animals. You can find good example of that on the cover of the May 1995 issue of J. Exp. Biol. It is a pity that no company (at least to my knowledge) makes anything like that anymore.) The main difference between microscopes in the imaging process for the human eyes is if there are one or two objective lenses. With one lens you get a "normal" LM, with two lenses you get a stereo microscope which needs objectives with long working distance (i.e. long focal distance and low N.A.) to make possible and meaningful to use stereo imaging. Of course if you attach a camera to a stereo micrscope you only use one of the objectives and consequently loose the stereo effect (which in any case is pointless since the camera is one-eyed).
Maybe this hasn't clarified things a lot, but at least I hope that "stereo microscope" might be used more rather than "dissecting scope" or the completely wrong Swedish word "lupp" which too many of my compatriots use (it basically just means magnifying glass!)
The main DNS server at ANL went on the blink a few days ago. Warning. You may see a rash of undeliverable mail. Hold off posting messages for a day or so until you see a new posting from me saying that everything has been corrected....
OR post at your own risk and be prepared for undeliverables.
For Sale JEOL 1200exII Conventional TEM. (straight TEM with no add- ons) This instrument is less than five years old and has been under service contract the entire time and is in mint condition. It has had limited users and all users were closely supervised by me; as a result it has had no down time. We are currently taking bids. send bids to: Susan Havrilesky Box 3209 DUMC Duke University Dept of Neurobiology Durham NC 27710
For more information call (919) 681-6425 or E-Mail me (Larry Hawkey) hawkey-at-neuro.duke.edu.
I'm interested in user's experience regarding the relative=20 performance of UTW vs Be window EDS detectors for the detection of high Z materials (i.e. rare earths). My own personal experience tends to favor the latter, although I can't, off hand, think of an inherent reason why this=20 should be so.
One reason that you might see better performance when acquiring spectra = from high-Z elements with a beryllium window, as compared to an = ultra-thin window, is that the detector with beryllium window may be = doing a better job of preventing back-scattered electrons from entering = the spectrum. =20
You can determine whether back-scattered electrons are the culprit by = examining the shape of the brehmstrahlung at the higher energy region. = Back-scattered electrons entering the detector will typically cause a = smooth "hump" in the spectrum above about 20keV.
If you find that this is the problem, you can try changing the = detector/sample geometry slightly, or check with the EDS manufacturer to = find out if an improved electron trap is available. =20
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
On October 3, Ritchie Sims asked about the SPI #02753-AB Mineral Standards and further asked if I would mind an open debate on this subject!
Of course I wound not mind! And if anyone has ideas on how we can make what we think is an outstanding product a bit better, I would thank you in advance for any such suggestions, publicly or privately.
Homogeneity of course is everything when it comes to mineral standards, and the individual mineral grains used in the mounts are selected with that particular thought in mind. The product is also very easy to use because of the built in Faraday cage and also the electron beam lettering opposite each mineral giving its identification, which can be "read" in the SEM or microprobe (but with mirror image lettering).
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
I=B4m looking for *graphical* simulation programs for Optics (Macintosh preferably, but others are welcome). I=B4m thinking of an application, which can be used like an optical bench used in physics (Optical axis where lenses, lasers, diaphragms, and else can be assembled). I would like to demonstrate the effects of:
- basic optical laws, refraction of light rays through lenses - diffraction effects and image formation (in microscopes) - diffraction patterns (light -} grid) - lens abberations (spherical, chromatic, distortions) - effects of depth of focus, etc.
in basic microscopy courses. Anything like that available? Thanks for any help, sincerely -Dietmar-
+++ Dietmar Reiter ++++++++++++++++++++++++++++++++++++++++++++ +++ Dept. of Zoology and Limnology office 1: (+43)-512-507-6170 +++ University of Innsbruck office 2: (+43)-512-507-6161 +++ Technikerstrasse 25 fax1: (+43)-512-507-2930 +++ A - 6020 Innsbruck, Austria fax2: (+43)-512-507-2957
... If I would have had more time, I would have written a shorter e-mail ...
{Text_1} I believe that the main difference between photomacrography and photomicrography is related to the optics used in obtaining the image. A micrograph is taken using a compound microscope (i.e. one where the image formed by the objective lens is further magnified by an eyepiece lens) whereas a macrograph is taken using a simple magnifier (i.e. single lens). At equal magnifications a macrograph will have greater depth of focus but lower resolution than a micrograph. Other differences relate to methodology. A photomacrographer usually approaches a subject and lights it according to photographic conventions (main light, fill, key lights etc.) using smaller than usual equipment such as dental mirrors, bits of foil, and small cards. The photomicrographer usually brings the specimen to the microscope and lights it as one would for routine observation (bright or dark field, epi, phase etc.).
Richard Mount
************************************************************ Richard J. Mount Phone: 416-813-6551 Auditory Science Laboratory FAX: 416-813-5036 Department of Otolaryngology The Hospital for Sick Children 555 University Avenue Toronto, ON, Canada M5G 1X8
On the Sep 22nd, I asked for sources of " 'non-bleaching', inorganic, specimens suitable for 1/ routinely checking excitation level and for 2/ correcting spatial uniformities of the optical/digital imaging system. - ". The query was posted to sci.techniques.microscopy, CONFOCAL-at-UBVM.CC.BUFFALO.EDU and microscopy-at-aaem.amc.anl.gov, and the summary is similarly posted as more than 20 persons asked for it. It runs 5 pages.
Some answers dealing with fluorescent beads are omitted. For 1/ and 2/ above, solutions made to desired specifications were recommended as flexible and traceable homogeneous standards, which do not bleach if a sufficiently large chamber is used, allowing fresh fluid to diffuse in under the beam.
Solid plastics of suitable fluorescence for flat-fielding is obtained from commodity stores; the exact sources were not specified, nor product name, composition or spectrum. None of the answers dealt with home-made fluorescent plastics, i.e. from epon or lowicryl.
Uranyl-glass is no longer produced, for environmental reasons, but available from distributors. Its radioactivity was not quantified, and probably assumed to be of no concern when the glass is used as microscope slides [should be tested?] Other inorganic standards than uranyl glass were not suggested. Here are the original answers, slightly edited.
********************** Uranyl and other inorganic standards **** several messages refer to Newport Industrial Glass, Inc 1631 Monvrovia Avenue, Costa Mesa, CA 92627, Tel 714-642-9980, Fax 714-645-6800, Bill Larsen. I phoned them and was transferred to Ray Larsen (brother) who immediately faxed an offer for microscope-slide sized glass plates, with curves for percent transmittance at different wavelengths (no fluorescence spectra. There are two qualities of glass (#3750 with refractory index, n= 1.509 and #3780 with refractory index, n= 1.533).
Date: Mon, 6 Feb 1995 09:52:54 -0800 From: George M {George_M-at-image1.com Uranyl glass slides - - - from Newport Industrial Glass Inc.[ - - -- - ] Sold as a 6.5x6.5" sheet (you can specify 1 mm height), so you may want to form a "consortium" to have Newport pre-cut a sheet to slide-size. - - - If there is a lotof interest, my company may start selling single slides. Dr. George McNamara Universal imaging corporation
Date: Wed, 5 Jan 1994 09:27:20 -0500 From: "Bill Bug (Bill bug)" {bbug1-at-CC.SWARTHMORE.EDU You can obtain micro slide sized pieces of uranyl glass from: Newport Industrial Glass Inc [- - - - - - - - -] They have this glass (glass #3750) in large stock pieces, so it must be cut down to the size of a micro slide. They will grind it down to whatever thickness you desire as well. We ordered two micro slide sized pieces in July of 1993. The cost was $86. Bill Bug, Department of Biology, Swarthmore College
[** Added by FMH: From Schott Glasswerke, Postfach 2480 D-55014 Mainz, Germany, Tel +49 61 31 660, Fax +49 61 31 66 20 00, we were referred to Schott Glass Technologies, Inc, 400 York Avenue, Dureya, PA 18642, Tel +1 717 457 7485, Fax +1 717 457 6960, Ref. Mr Steve Sokach. The latter company makes Rare Earth Doped Filter Glass with specified absorption spectra, but so far no data on fluorescence has been received. A fax to the latter number brought no answer so far.**] Finn-Mogens Haug
University of Oslo Institute of basic medical sciences Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no Box 1105 Blindern Phone : +47 22 85 12 67 N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
I regret to inform you that the ANL (Argonne National Lab) link to BITNET is being terminated. The Microscopy Server will nolonger be able to forward mail via BITNET. If you are recieving mail via this route you will have to change your service provider and resubscribe with your new address.
Just a technical, maybe obvious note coming from experience: the thinner the window, the easier it fails as a vacuum seal which means several weeks of repair and inconvenience. Take this into consideration when choosing. Nick Schryvers
I have been following the comments about macrography and micrography with much interest. Of particular interest to me are the comments concerning some sort of standardization of usage. ASTM does have at least one standard (E7-95 Standard Terminology Relating to Metallography) which could be used
MACROGRAPH - a graphic reproduction of an object, slightly reduced in size, unmagnified, or magnified ten diameters or less (photomacrograph).
MICROGRAPH - a graphic reproduction of an object as seen through the microscope or equivalent instrument, at magnifications greater than ten diameters (photomicrograph).
While these definitions are not perfect - they are contained in an accepted standard - thus they may be of use. Note there are also some older ASTM standards which are no longer in service that contain definitions relating to micrography and macrography.
The New York Microscopical Society has published a GLOSSARY OF MICROSCOPICAL TERMS AND DEFINITIONS which contains a set of less consistent definitions.
Are there any MSA documents which could be used to standardize the usage of such terms ?
Mark E. Cavaleri 3M CRL/A&PRL Light Microscopy 3M Center 201-1E-15 St. Paul, MN 55144-1000 (612) 733-3247 (612) 733-0648 FAX mecavaleri-at-mmm.com
} There are a number of issues relating to the choice between UTW and } Be windows. Some of these are: } ... } Count rates: The light elements can contribute a significant number of } counts to the spectrum, especially in a TEM. These can increase the } throughput of the pulse processor, degrading the resolution and increasing } the dead time. Since the low-energy counts have to be recorded, the pulse } discriminators must be set down into the noise, further increasing the number } of counts that must be processed. Hence, depending on the samples, the } number of counts recorded from the heavier elements will be less for a UTW } detector running at maximum count rate than for a Be-window detector in } the same situation. } ... } Tony. } ********************************************************* } * Anthony J. Garratt-Reed * } * Massachusetts Institute of Technology, Rm. 13-1027 * } * Cambridge, MA 02139, USA * } *********************************************************
I am curious obout your statements on count rate. Certainly the Be window would attenuate the low energy pulses including the light elements and low-end noise so that the pulse processor would not have to process them at all. You said that the low-energy counts have to be recorded with a UTW detector. What about just turning up the fast discrminator to throw away the light element x-rays in addition to the noise? How much of a burden would that put on the pulse processor as opposed to the case with a Be window which absorbs those x-rays? ---------------------------------------------------- Warren E. Straszheim, Ph.D. 270 MD Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091 E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
coal characterization and processing electron microscopy, x-ray analysis, image analysis computer applications
I am looking for input from list members on high resolution laser printers for printing digital images from our Auger systems. We have the ability to capture to a PC, and would like to print out these images. The quality of the image system is not real good, so the laser will probably provide sufficient resolution. The Auger system software also limits what we can use. The images must be printed off the print command from the PC since the software contains the mag and title data (not part of the image file). If it was a part of the file we could send data to our SEM PC which is connected to a Codonics VP4500 thermal printer.
Any information/experiences from the list members on laser printers would be greatly appreciated. You can reply to the list or directly to me.
Thanks,
John Giles (jegiles-at-space.honeywell.com) Honeywell Space Systems Clearwater, FL
Nick Schryvers has pointed out that ultra thin windows fail easier than beryllium windows. While this is true to some extent, ultra-thin windows are extremely reliable. The last time I compiled data in this area was around May 1993 for my article on UTW's in "X-ray spectroscopy in electron-beam instruments." At that time mean time before failure was seven and a half years and rising. (rising because we have only had windows in the field since about 1990). Many of the field failures were due to causes that would also have broken a Be window. Some microscopes are harder on UTWs than others,due to the specific flow of turbulance around the window during venting, but these problems have been identified and fixes are available.
Tony is right that a Si(Li) detector can only handle one event at a time, whether inside or outside of the region of interest. Opening the detector to soft x-rays will increase the counts processed whether discriminated out by the electronics or not.
For those of you that read this I have shut down the server to reconfigure the DNS service. I've waited for the queues to clear and will see how many Undeliverables I get with this message. If it's only a few then the problem is cured. If not then it will be another day or so...
The problem arose due to a change in the DNS service here at the lab and the software that ANL uses to move mail from their various computers out to the net. I use Multinet on this computer and Multinet has had to be reconfigure to match the local and net wide changes....
I've made a fairly massive change last night. I need yet another test message, I hope it's the last. I think everything is okay and we will be back on-line shortly. With a list this size I do not want to start another round of bouncing mail.
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
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----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
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----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
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----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
CAn anyone let me know the email address for David Smith at Arizona State U, Center for Solid State Science. Thank you in advance Cheers jjm Professor John J. Millar Head, Department of Applied Physics Electron Microscope Unit RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001 +613 660 2602 fax 613 660 3837 email jjmill-at-rmit.edu.au
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
Jerome - Roger Johnson just finished a thesis here on a conversion of an SEM for x-ray tomography and microscopy. We had a short paper at EMSA?MSA Boston in 1992.
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----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
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----------------------------------------------------------- Nestor J. Zaluzec
Microscopy Society of America TeleCommunications Office Email: Zaluzec-at-MSA.Microscopy.Com -----------------------------------------------------------
************************************************************ Welcome to ************************************************************
Microscopy-at-MSA.Microscopy.Com
************************************************************ Hosted and Sponsored by The Microscopy Society of America (MSA) as a Free Service to the World-Wide Microscopy & Microanalysis Community ************************************************************
G'day Subscribers....... (Well at least I hope it is day-time, when you read this )
It looks as if we are now back on-line, and as the subject title and the above banner indicate some major changes have occurred during the last few days. You might even say we now have a new face, well at least a new envelope !
Firstly, as most of you know I had been running the Microscopy Listserver at ANL for just over 2 years, basically without any real support, save my time and some electricity. As a consequence it was becoming harder and harder to keep things running properly, let alone efficiently.
At this time it is my pleasure to officially announce that the Microscopy Listserver has moved (hopefully, nearly transparently to you but obviously with a bit of work for me) to a new address and with a new sponsor.
MSA at their 1995 Winter/Spring Council meeting, voted to establish a full internet site for the society and it's members and to host the Microscopy Listserver as a FREE service to the microscopy and microanalysis community worldwide. It has taken some time to get everything running, but as of last night, the listserver is now relocated and functioning with a modicum of success. I still basically do the majority of the work (gratis), however, we now have a new workstation (Sun Sparc5), software, and as I mentioned an offical internet site. The server will still need some work, but we now have the resources to improve the system and make it more user-friendly.
For example, with the new configuration of header (i.e. the envelope of this Email message), errors and undeliverables should now no-longer be returned to the originator, but are sent to the SysOp ( Ijust need more mail, right?). In addition, I will be bringing up on-line some of the more usual features characteristic of listserver software in the coming weeks . I will post the information to to the list as the options become functional.
I would encourage you to send thanks to MSA Council for saving the listserver from a slow death, as the hardware and software configurations at ANL were becoming increasing difficult to maintain and as some of you know were beginning to get particuliarly ornery. From my own perspective, my thanks go to all the members of council for establishing this site, and a special thanks to two members who have been especially supportive namely- Ron Gronsky of UC Berkeley and Ron Anderson of IBM.
The general MSA Email Address is:
MSA-at-MSA.Microscopy.Com
while the business office can be reached at:
MSABusinessOffice-at-MSA.Microscopy.Com.
Additional information about MSA, the society and it's operations can be found at the MSA WWW site:
http://WWW.MSA.Microscopy.Com
MSA also mirrors the MMSLIB (Microscopy & Microanalysis Software Library) anonymous ftp site at:
FTP.MSA.Microscopy.Com
and as you already know the Microscopy Listserver at:
Microscopy-at-MSA.Microscopy.Com
Mail from the old server site (Microscopy-at-AAEM.AMC.ANL.GOV) will be automatically forwarded to the new server site for awhile, but please change your local aliases and/or shortcuts, so that your mail goes directly to the MSA site instead of channeling through ANL.
As I mentioned, there will still be a few growing pains, but the hard part should be basically done.
In addition to the move from the ANL to the MSA site, the listserver has passed another milestone this weekend, the 3,000th subscription request arrived, not bad for only 2 years of operation. The lucky winner of a beer should he and I ever meet is:
Russell J. Wilson Analytical Electron Microscope Facility Queensland University of Technology Garden Point G.P.O Box 2434 Brisbane Qld. 4001.
Russell, it's your job to make sure that a cool slab of tinney's is around, but I'll buy!
BTW if you are a triva buff or just curious the list sends Email to Microscopists in 39 countries around the world. Over 5600 Email messages have been submitted to Microscopy over the period Oct 1, 1993 to Oct 1, 1995. Not all of them have been gems, but there have been occasional useful bits of info......
Message-Id: {199510110507.AA21791-at-shrike.adl.soils.csiro.au} X-Sender: mcc332-at-shrike.adl.soils.csiro.au X-Mailer: Windows Eudora Pro Version 2.1.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
If this is any help to you. There was a paper that I remember seeing about 10 yrs ago that used a thin metal film to generate xrays from the electron beam which were then used as a point source to pass through the specimen onto xray film. Magnification set by the source-specimen :specimen-film distance. ie e e e m-metal-m x x x ss-specimen-sss x x x x fffff--film--fffff
I can't at this moment find the reference but I can have a good dig if you want.
stuart
} } } } Several years back I read about a group that interposed an X-ray source } } within a STEM or SEM. Electrons hit the target, generating X-ray's which } } could be used to visualize the specimem producing both an electron image } } and an X-ray image of the same sample. I have searched the literature and } } cannot find a reference to this. Does anyone know of a reference or the } } existence of such a technology or am I just getting senile? } } Your help and assistance with either of these two issues (the scope or my } } senility) would be appreciated. } } } } Thanks-
} } Jay Jerome * jjerome-at-isnet.is.wfu.edu * } } **************************************************************
--------------------------------------------------------------------------- Stuart G. McClure, | Post small : P.B.#2, Glen Osmond, CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064. Adelaide Laboratories, | Post large : Waite Rd, Urrbrae, SA, Australia | Sth Australia, AUSTRALIA, 5064.
Phone: (08) 303-8484 International use +61-8- instead of (08) Fax: (08) 303-8550 Email: Stuart.McClure-at-adl.soils.csiro.au ---------------------------------------------------------------------------
I am looking for names and address (email or fax) of companies who make acoustic microscopy for the materials sciences research.
Best regards for
Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl} Foundry Research Institute Research Department - Structural and Physical Research Laboratory Zakopianska 73 Call +48 12 605022 ext. 356 30-148 KRAKOW - POLAND Fax +48 12 665478 :-)
Hi, You can try using graphicConvertor a shareware program that lets you convert several mac/ibm formats. It's available from several sites, you can look in zippy.nimh.nih.gov /pub/nih-image/programs using anonymous ftp. If its not in programs its in one of the subdirectories of nih-image. By the way NIH-Image itself is well worth a look, it has some import capabilities (but doesn't handle all types of TIFF), and it allows a range of image analysis techniques as well as 3-D reconstruction etc.
Michael A. George, Ph.D. ******************************************* Research Assistant Professor Department of Physics Fisk University Nashville, TN 37208
To update and clarify my message of yesterday, we are looking to -} purchase {- a used H-600. We are pretty much wed to the idea of staying with Hitachi products.
Thank you for responses received to date.
Donald L. Lovett e-mail: lovett-at-trenton.edu Asst. Professor, Dept. of Biology voice: (609) 771-2876 Trenton State College, NJ 08650-4700 fax: (609) 771-2674
on selling a used microscope? We have a light microscope for sale and want to check first before we break any rules! I have seen SEM's and the such offered and want to see if it is OK .
We are a small, state, liberal arts university with a very limited budget. I just had an SEM donated to our department and am now in need of a sputter coater and critical point dryer. If anyone has either of these pieces of equipment not currently being used or knows where I could get them (preferably as a donation) please let me know. Thank you.
Marty Levin Department of Biology Eastern Connecticut State University Willimantic, CT 06226
First, I want to thank Nestor (Our Friendly Neighborhood SysOP) for all his untiring efforts. I would also like to thank MSA for agreeing to support the list. (Very tongue in cheek) I am sure both will receive their just rewards.
BUT... to be the first of many to ask this.... with the change of listserver.. how does one Subscribe/Unsubscribe. That faciously, is a request for information on the correct address of the Listserver and the commands needed to get it to do what we would like it to do.
Again thank you to you Nestor for your time and effort.
Greetings! The reference(s) to which you refer Jay are:
Middleman L.M. and Geller J.D. Scanning Electron Microscopy (1976), I, 171 -179 Eckert R. Scanning, 8, 232 -238, (1986)
I've tried it - and it definitely works ( not unexpectedly! ). The problem is spatial resolution; now, however, there has been significant improvement in x-ray focusing optics and several groups ( including ours ) are in various stages of constructing useful ( hopefully! ) scanning x-ray fluorescence microscopes.
Does anyone have used heavy metal staining (OsO4) on rock samples (prepared as polished sections) in order to detect the occurrence of organic material ? Where could I find the staining procedure ? Thanks in advance.
Long Liang ARCO EPMA/SEM Lab Plano, TX lliang-at-is.arco.com
We will be dismantling an EMX-SM microprobe in the next few weeks. It hasn't been used in 2 years but has been kept under vacuum. Along with the microprobe itself, I have plenty of spare components and parts. Anyone is welcome to all or any of the components for the cost of shipping.
Contact me directly if interested.
Thanks, Lou Ross
101 Geological Sciences Bldg. University of Missouri Columbia, MO 65211 (314) 882-4777, 882=5458 fax
I have finaly taken the time to organize my own collection of icons, and collect a few microscopy related ones from other sources. There are also a few small, and well colored icons that I have found to be very useful for teaching.
The appropriate link is: http://risc1.numis.nwu.edu/internet/Icons
Notes: 1) Some of the directories are quite large and may be very slow if you have a slow modem connection. 2) Contributions are welcome - please contact me at l-marks-at-nwu.edu . 3) I am receptive to the idea of mirroring some of these icons in Europe and Asia to improve access.
Acknowledgements
I would like to thank: Pierre-Henri Jouneau, Centre Interdepartemental de Microscopie Electronique David Dryden, , University of Melbourne Daniel L. Callahan Department of Mechanical Engg. and Materials Science, Rice University JEOL, USA Nissei Sangyo Canada, Inc
Message-Id: {199510111247.NAA05175-at-pons.uio.no} X-Sender: finnmog-at-pons.uio.no X-Mailer: Windows Eudora Version 1.4.3 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Their adress was cited by the Thomas register as Flow Cytometry Standards Corporation 513 Hostos Avenue, Ste. B3 P.O. Box 194344 San Juan, Puerto Rico 00919
but TR could not find any phone/fax numbers. Thanks in advance to those who send the numbers by direct e-mail (and I will post the numbers to the list, once). Regards from
Finn-Mogens Haug
University of Oslo Institute of basic medical sciences Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no Box 1105 Blindern Phone : +47 22 85 12 67 N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
I do not have addresses or emails ids readily available but I can give you companies who make them:
Sonoscan Hitachi Olympus Sonix
Hope this helps.
--- Forwarded mail from Krzysztof Hubner {zahubner-at-cyf-kr.edu.pl}
Dear Friends,
I am looking for names and address (email or fax) of companies who make acoustic microscopy for the materials sciences research.
Best regards for
Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl} Foundry Research Institute Research Department - Structural and Physical Research Laboratory Zakopianska 73 Call +48 12 605022 ext. 356 30-148 KRAKOW - POLAND Fax +48 12 665478 :-)
---End of forwarded mail from Krzysztof Hubner {zahubner-at-cyf-kr.edu.pl}
I am looking for names and address (email or fax) of companies who make acoustic microscopy for the materials sciences research.
Best regards for
Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl} Foundry Research Institute Research Department - Structural and Physical Research Laboratory Zakopianska 73 Call +48 12 605022 ext. 356 30-148 KRAKOW - POLAND Fax +48 12 665478 :-)
Greetings: I just received the RMS Proceedings, vol. 30 part 3 (Sept. 1995). The article on page 194 describes the "PTL Pro 100 Macro Scanning System" but there is no information on who manufacturers or distributes the system. Any information would be most appreciated. Thanks, Doug ***************** Douglas D. Stokke USDA Forest Service North Central Forest Experiment Station Forestry Sciences Lab, SIU-C Carbondale, IL 62901-4630 PH# 618-453-2920 FAX 618-453-2911 e-mail: dstokke-at-siu.edu Forest Service DG: D.Stokke:S23L01A
Image convertion: About the discussion on image convertion, the Share ware (usesr should pay small fee to keep this type of service upcoming) {Graphic Converter} can be downloaded from http://hyperarchive.lcs.mit.edu/HyperArchive/Archive/gst/grf/graphic-converter-215.hqx Make sure that you have a working copy of {Stuff it} (also share ware) in you computer so that the file is automatically decompressed from its binhex format. Graphic Converter is truly outstanding for its price ($35.00).
****************************************************************** *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services * ******************************************************************
I use Syquest removable media (44MB each) for image storage in the Mac and the PC. I purchased DOS MOUNTER from DAYNA Communication, inc and can mount easily floppy DOS-MAC. Are there any drivers out there (software?) that would allow me to bring a DOS formatted Syquest to the MAC without having to update the driver every time is inserted across plataforms? Thanks in advance.
****************************************************************** *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services * ******************************************************************
Thank you to all those who replied. Received and understood. Cheers jjm Professor John J. Millar Head, Department of Applied Physics Electron Microscope Unit RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001 +613 660 2602 fax 613 660 3837 email jjmill-at-rmit.edu.au
Thank you to all those who replied. Received and understood. Cheers jjm Professor John J. Millar Head, Department of Applied Physics Electron Microscope Unit RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001 +613 660 2602 fax 613 660 3837 email jjmill-at-rmit.edu.au
In the late 70's I saw this method used at a hospital in Calgary Canada. They had modified a Cambridge S180. The stage had a target for X-ray generation with the specemin (a hamster eyeball) beneath it. Below that was a minature 35mm camera they had manufactured. I hope this will help you. Check with Cambridge / Leica for S180 user in Calgary.
} } } Several years back I read about a group that interposed an X-ray source } } } within a STEM or SEM. Electrons hit the target, generating X-ray's which } } } could be used to visualize the specimem producing both an electron image } } } and an X-ray image of the same sample. I have searched the literature and } } } cannot find a reference to this. Does anyone know of a reference or the } } } existence of such a technology or am I just getting senile? } } } Your help and assistance with either of these two issues (the scope or my } } } senility) would be appreciated. } } } } } } Thanks- } } } } Jay Jerome } * jjerome-at-isnet.is.wfu.edu * } } } ************************************************************** } Regards Derek Simpson
Message-Id: {199510111605.LAA27683-at-bcm.tmc.edu} X-Sender: joiner-at-bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Congrats and kudos on the move of the listserver. We all appreciate the work that you've done. HOWEVER.....You were waiting for that, weren't you?....Your message: "Microscopy is back on-line with some major changes!" came across, to my machine at least, in what appears to be two parts. The first ended with your paragraph asking us to thank the MSA Council, and no signature. The second part came as a second message begining in mid-sentence without an envelope. I knew it was from you because of the signature at the end. There appears to be a chunk of message missing from the middle. I believe that you said in this message that the new address for messages to the listerver is: microscopy-at-msa.microscopy.com. Is this correct? But what I got did not specify an address for subscribe/unsubscribe requests. Is there one? I would like to wait to change my aliases until I have confirmed these addresses.
Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy Department of Pathology, Rm.286-A Baylor College of Medicine One Baylor Plaza Houston, Texas 77030 U.S.A. tel.: (713)798-4658 FAX: (713)798-3945 joiner-at-bcm.tmc.edu Compuserve: 71555,1206
From early on I noticed that not all apps treat TIFF images the same. Pagemaker under Windows could read my files while Word could not. I had similar experience on the MAC. Some MAC apps could read my images while others could not. A lot depended on the converters in the app. It was not a Mac ves. PC issue, but rather what the TIFF structure was. Both Mac and PC apps _should_ be able to read the same TIFF file. For a while I used Graphics Workshop to convert the file to a new TIFF file and the resulting file sometimes worked in more apps.
I am not sure if I completely understand the workings now, but I have gotten most of the tags straightened out (TIFF = Tag Image File Format). If I recall, I am now overspecifying the image with the tags I now include (e.g., (size of image and hor.-dimension and vert.-dimension). But if that is what it takes to keep more apps happy, then so be it.
In summary, I do not have any suggestion for converters. I would be interested in what you find out. I suppose that it may take a reputable commercial product to be robust enough to handle all apps. But even then, I would test the images on the apps of concern.
We have been given a DAPI filter cube to trial prior to purchase. I have two questions regarding its use :
1. Different objective lenses give different images. Why? 2. General uses for a DAPI filter cube. Can we justify the cost?
------- 1. When we observe our specimen (macrophages within lung - cryostat sections) using a 40x Pl Fluotar objective 1.00-.50 oil (LEICA lens), our images are superb : clear, crisp, pretty. This is the case both with oil immersion and just dry viewing using this lens.
The problem occurs when we view our sample with our 60x Pl Apo objective 1.4 oil (LEICA lens). The image becomes non-existent or ghost-like. A pale "shadow" only is seen, with no detail visible at all. However, viewing with green and blue excitation lines (fluorescence microscope) continues to give excellent (although non-specific fluorescent) images on both the 40x and 60x objectives.
I discussed our problem with LEICA. At first thought, the explanation they proposed was that the 60x Pl Apo was giving autofluoresence from the glass components of the objective lens following excitation with ultraviolet light (selected by the DAPI filter). This then masked the sample fluorescence to the extent that no image was obtained. The longer wavelengths (green and blue lines) would not cause this autofluorescence.
In comparison, the Pl Fluotar 40x objective could cope with a larger range of wavelengths, hence this problem was not observed with this lens.
IS THIS A REASONABLE ASSUMPTION? HAVE OTHER PEOPLE EXPERIENCED SIMILAR DIFFICULTIES? ------- 2. Our application is visualisation of marcophage particles in lung tissue. Macrophages autofluoresce but we are trying to locate the presence of benzopyrene within the cells. Benzopyrene excites at 380nm and emits at 430nm. The DAPI filter seems a reasonable choice (and was recommended by this discussion list).
WHAT ARE OTHER PEOPLE USING DAPI FILTERS/STAINS FOR? ARE THERE MANY OTHER GENERAL APPLICATIONS FOR DAPI THAT COULD HELP JUSTIFY THE PURCHASE PRICE OF ~$650 (AUSSIE DOLLARS)?
Many thanks for your responses,
Felicity Lawrence Analytical Electron Microscopy Facility, QUT Brisbane, Australia
For the moment, the procedure to subscribe and/or unsubscribe remains the same. Just send a Email message to:
Listserver-at-MSA.Microscopy.Com
in the body of the message type the following
Subscribe Microscopy {YourUserID-at-YourDomain} or Unsubscribe Microscopy {YourUserID-at-YourDomain}
just like before. The only difference is the MSA Internet Address, and yes the old address for this also forwards from AAEM.AMC.ANL.GOV to MSA.Microscopy.Com.
I need a bit of time to prepare new documentation and the like, but when it's ready you will all get a copy. For the present most things run in a similiar procedure to the old system, except possibly fewer error messages. BTW the "rules and etiquette" on this list remain the same as before, remember it's the same list, just a new address.
You will all also continue to see the random subscribe/unsubscribe messages which are incorrectly posted to the "Microscopy" address instead of the "Listserver" address. We've already had several in the last few days. That I can't do anything about, unless the list becomes "Moderated" which means someone must read and approve each and every message. Sorry folks, but there is no way I have time to do that...
You should also be able to now easily recognize Email from the listserver by looking at the Sender's address in your Email program. Each mail message which is "distributed" by this server has a new envelope prepended to it. Thus, all mail from here will now have a From: line which looks like:
while a few lines down in the header you will be able to locate the name of the person that posted the original message something like this
If you have a smart Email client program, you can use this new
You might also notice that the Domain name appears as Sparc5.Microscopy.Com. instead of MSA.Microscopy.Com. This is a fluke of the SUN system software. The computer which I am running on is called locally Sparc5 and the sendmail program appends the Domain name of just Microscopy.Com. which is the offical domain for the site. The name MSA.Microscopy.Com is officially registered to this particuliar node (206.69.208.10) but so is the local name of Sparc5. It appears that the local name takes precedence on mail being sent out. To change the local name will mean a reconfiguration of all accounts on the system and I don't have the time for that. I'll figure out a way of cleaning this up eventually. The sender name of "Microscopy-request" has to do with the alias system for the list and the way I've set up the initial system.
I am beginning to consider low-cost steps which we can take to further reduce the number of airborne (nuisance) particles in our optical and infrared microscopy lab (approx. 2400 cu. ft, 240 sq. ft.). This is a desire, not a necessity, so cost is a _big_ factor. I see portable air cleaners and bench clean air hoods as two possible options.
Any others? Does anyone have sufficient experience with portable air cleaners to make recommendations? Does anyone have a nifty design for low-cost self-built bench clean air hoods?
In response to Ritchie Sims, I was under the impression that most Edwards 306 diffstak pumps used Santovac 5. BE SURE to check this out with Edwards before proceeding. Santovac 5 takes a lot higher heat to operate than DC704, they are not mutually interchangeable.
Operating a silicone oil at a heat much above its designed range is dangerous not just from a contamination point of view but could result in an explosion or fire.
You can operate Santovac 5 in a pump built for DC704, you will just suffer slower pumping speeds.
Dear Felicity: Your Leica lens may not transmit in the region of excitation as well as your other lens. (My best guess).
Dapi filter is very useful. You can stain DNA in nucleii and mitochondria with the dye DAPI, lignin in plant cells is best observed with the Dapi filter and there are many other uses. Regards, Nina Allen
So you're getting rid of the ARL. Have you picked up another probe?
Owen At 02:07 PM 10/11/95 -0600, Lou Ross wrote: } Hi, } } We will be dismantling an EMX-SM microprobe in the next few weeks. } It hasn't been used in 2 years but has been kept under vacuum. Along with } the microprobe itself, I have plenty of spare components and parts. Anyone } is welcome to all or any of the components for the cost of shipping. } } Contact me directly if interested. } } Thanks, } Lou Ross } } 101 Geological Sciences Bldg. } University of Missouri } Columbia, MO 65211 } (314) 882-4777, 882=5458 fax } } } Owen P. Mills Michigan Technological University Metallurgical & Materials Engineering Rm 512 MME Building Houghton, MI 49931 906-487-2002 906-487-2934 FAX opmills-at-mtu.edu
I am having trouble converting a stack generated by a Bio-Rad confocal to a format the XEVA visual studio recognizes. Any suggestions?
Thanks in advance, Tom Thielen
******************************************************************************* *Tom Thielen "People who make sweeping generalizations are stupid!" * *Pre-Med Major * *Winthrop University thielent-at-lurch.winthrop.edu * *******************************************************************************
I'm looking for a dual adaptor for my inverted Nikon Diaphot. I need to attach two CCD cameras to the camera port simultaneously. The only product I'm aware of costs an arm and a leg (produced by Nikon, of course). All I need is an adaptor that has room for a dichroic mirror and a couple of emission filters, with standard C-mount threads.
If anyone knows of a source for this type of device, I'd appreciate it.
Thanx Mike Tymianski Toronto, CANADA mike_t-at-camtwh.eric.on.ca (416) 503-5868 .
I've already sent Brendon a personal response about converting PC TIFF images to MAC TIFF, but I thought I would post it to the group.
I've been using MacLinkPlus/PC Connect for several years to transfer IBM TIFF images to a Mac though the serial line. It works great, I've never had a problem. Included are all types of translators for both platforms and desktop translators for each platform. (ie, Word to Wordperfect)
The company is Dataviz, 55 Corporate Dr., Trumbull CT, 06611 (800) 733-0030. It goes for ~$130 with annual updates for ~$40.
Hope this helps. Lou Ross
101 Geological Sciences Bldg. University of Missouri Columbia, MO 65211 (314) 882-4777, 882=5458 fax
Hello all, I'm new in this area of science and need some assistance:
We are in need of a 14'x11.5' section of darkroom cloth for seperating one room with the ability to do light free analysis. We have ran into a wall with our catalogs and resources in locating a vendor for such a curtain. Could someone advise us on where we might find such a vendor or suggest other solution that might have been done in the past.
Thank you in advance, Davin
Davin B. Jutila Flow Cytometry/Research Imaging Facility Southern Illinois University School of Medicine 801 North Rutledge Mail Code - 1220 Springfield, IL 62794-9230 Ph#: (217)-782-0898 Fax#: (217)-524-3227 e-mail: djutila-at-wpsmtp.siumed.edu
} Does anyone out there know where a person might be able to locate at } source for light microscopy 'well - slides'? } Richard, Would hanging drop slides serve your purpose? If so Fisher has these. Pp.1229 in their '95/'96 catalogue. They have 1 or 2 cavities in normal and extra thick. Order no. 1-800-766-7000. Hope this is helpful.
Sandra Zane Sandra F. Zane, EM Tech. Dept of Biol., UNC Charlotte 9201 University City Blvd. Charlotte, NC 28223-0001 sfzane-at-unccvm.uncc.edu Fax (704) 547-3128
We use air purifiers in our darkrooms and it helps a lot} This is especially true in the winter when in the past we have spent hours fighting the dust battle to only find more dust on the neg when printing.
Being an asthmatic, and on their newsgroup, I've seen tons of postings on the subject, and have researched it myself.
The best portable air purifier in most ( and my opinion) is the Honeywell envirocare HEPA air purifier. We have ionizers in our darkrooms, and they give off ozone, and snap a lot, they also cost about $250 each. For Home I bought a Honeywell instead after experiencing the ionizers.
The Honeywell HEPA is 99.97% effecient down to the 0.3 micron range, has a low white noise that isn't bad, and smells really fresh. ie I'll stand by my honeywell all day, but I don't want to get near those ionizers , yuck.
Cost: Depends upon model, 3 sizes that I know of, about $130-$225
Maintenance: Change charcoal prefilter once every 3 months ( pretty cheap) Change main filter once every 2-5 years.- a little more $$
Hope this helps some,
Lou Ann -------------------------------------------------- Any others? Does anyone have sufficient experience with portable air cleaners to make recommendations? Does anyone have a nifty design for low-cost self-built bench clean air hoods?
Thanks.
James Martin
*********************** Lou Ann Miller Microscopic Imaging Laboratory College of Veterinary Medicine University of Illinois 2001 S Lincoln Ave Urbana, Illinois 61801 217-244-1566 lmiller-at-ux1.cso.uiuc.edu
Neuroscience List {neur-sci-at-net.bio.net} , Morphmet List {morphmet-at-cunyvm.cuny.edu} , MolecularCellSpeak List {molecular-cell-speak-at-mailbase.ac.uk} , Microscopy List {microscopy-at-aaem.amc.anl.gov} , Confocal Microscopy List {confocal%ubvm.BITNET-at-BITNIC.CREN.NET} , Cell Bio List {cellbiol-at-net.bio.net} , Biz-Biotech List {biz-biotech-at-netcom.com} , Biomechanics List {biomch-l-at-nic.surfnet.nl} , Biomaterial List {biomat-l-at-hearn} Message-Id: {Pine.3.89.9510121449.A28707-0100000-at-pauline.sdsc.edu} Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII
I am in need of a 3D physical model (plastic, etc.) of a cell for demonstration. Any cell type will do. Does anyone know of manufacturer of such an item for purchase?
Please mail me directly and not the list itself: (BRANDE-at-SDSC.EDU). Thanks in advance for your help.
Marc
Marc C. Brande, M.S. SD3D Email List:3D Imaging San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu 3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830
I have been using AccessPC by Insignia Solutions in my Macs for several years and have no trouble with DOS 44 MB SyQuests mounting each time, flawlessly.
} I use Syquest removable media (44MB each) for image storage in the Mac and } the PC. I purchased DOS MOUNTER from DAYNA Communication, inc and can mount } easily floppy DOS-MAC. Are there any drivers out there (software?) that would } allow me to bring a DOS formatted Syquest to the MAC without having to update } the driver every time is inserted across plataforms? Thanks in advance. } } ****************************************************************** } *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * } *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * } *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * } *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * } * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services * } ******************************************************************
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
Sirs: If you prefer that future press releases be sent via snail mail, or to a different email address, please let me know. Two press releases follow. Lynna Howard
NEWS RELEASE FOR IMMEDIATE RELEASE September 25, 1995
New VoxBlastx Issued for Windows 95 and Windows NT
VayTek, Inc.'s software for rendering, measuring and presenting 3D data now takes advantage of the Windows 95 and Windows NT operating systems.
The new Windows versions are significantly faster on a Pentium computer than on the DX2 or DX4 computers. Its speed now rivals that of several of the UNIX workstations.
VoxBlast is a cut above most 3D volume visualization programs, offering powerful tools for extracting quantitative information. Arbitrary slicing planes, true 3D measurements, surface extraction, polygon rendering, flood fill capabilities, and volumetric calculations are just a few of the standard features.
Unlike most other 3D rendering programs, VoxBlast measurements are based on fractional interpolations and careful preservation of original data, so the end results are more accurate.
The Windows versions of VoxBlast retain the high degree of flexibility and compatibility of previous versions. Data sets from microscope data, CT scans, petroleum engineering data, chip inspection, and other sources are readily accepted, giving researchers a broad range of choices for ancillary equipment.
Sophisticated lighting models, movie generation features, and palette editors are included in the program. Presentaion and evaluation of data is enhanced when the user incorporates color, transparency, motion, and lighting: pseudocoloring is available from a 16 million-color palette; cutting planes can be presented with one palette and opacity table on one side, and an entirely different palette and opacity table on the other side; the transparency feature will reveal details in the data set that would otherwise be hidden. All of these features and many more can be used in self-scripted movie loops.
Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast runs on less expensive hardware.
VoxBlast is available on the Internet for evaluation. Contact VayTek at 515-472-2227 or vaytek-at-ins.infonet.net for download instructions.
xxx
NEWS RELEASE FOR IMMEDIATE RELEASE September 25, 1995
VoxBlastx Now Native for Power Mac
VayTek, Inc.'s software for rendering, measuring and presenting 3D data now runs approximately three times faster in the new, native version for Power Macs.
With this dramatic increase in speed, the Power Mac can match many UNIX workstations in accepting a stack of registered 2D images for creation of 3D projections.
VoxBlast is a cut above most 3D volume visualization programs, offering powerful tools for extracting quantitative information. Arbitrary slicing planes, true 3D measurements, surface extraction, polygon rendering, flood fill capabilities, and volumetric calculations are just a few of the standard features.
The native version of VoxBlast retains the high degree of flexibility and compatibility of previous versions. Data sets from microscope data, CT scans, petroleum engineering data, chip inspection, and other sources are readily accepted, giving researchers a broad range of choices for ancillary equipment.
Researchers at Pioneer Hi-bred in Iowa say, "For the price, Voxblast has no equal." University of Missouri scientists add, "In addition to the high quality images, we were impressed by the flexibility of VayTek's software."
Unlike most other 3D rendering programs, VoxBlast measurements are based on fractional interpolations and careful preservation of original data, so the end results are more accurate.
Sophisticated lighting models, movie generation features, and palette editors are included in the program. Presentaion and evaluation of data is enhanced when the user can incorporate color, transparency, motion, and lighting: pseudocoloring is available from a 16 million-color palette; cutting planes can be presented with one palette and opacity table on one side, and an entirely different palette and opacity table on the other side; the transparency feature will reveal details in the data set that would otherwise be hidden. All of these features and many more can be used in self-scripted movie loops.
Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast runs on less expensive hardware.
VoxBlast is available on the Internet for evaluation. Contact VayTek at 515-472-2227 or vaytek-at-ins.infonet.net for download instructions.
Sirs: If you prefer that future press releases be sent via snail mail, or to a different email address, please let me know. Two press releases follow. Lynna Howard
NEWS RELEASE FOR IMMEDIATE RELEASE September 25, 1995
New VoxBlastx Issued for Windows 95 and Windows NT
VayTek, Inc.'s software for rendering, measuring and presenting 3D data now takes advantage of the Windows 95 and Windows NT operating systems.
The new Windows versions are significantly faster on a Pentium computer than on the DX2 or DX4 computers. Its speed now rivals that of several of the UNIX workstations.
VoxBlast is a cut above most 3D volume visualization programs, offering powerful tools for extracting quantitative information. Arbitrary slicing planes, true 3D measurements, surface extraction, polygon rendering, flood fill capabilities, and volumetric calculations are just a few of the standard features.
Unlike most other 3D rendering programs, VoxBlast measurements are based on fractional interpolations and careful preservation of original data, so the end results are more accurate.
The Windows versions of VoxBlast retain the high degree of flexibility and compatibility of previous versions. Data sets from microscope data, CT scans, petroleum engineering data, chip inspection, and other sources are readily accepted, giving researchers a broad range of choices for ancillary equipment.
Sophisticated lighting models, movie generation features, and palette editors are included in the program. Presentaion and evaluation of data is enhanced when the user incorporates color, transparency, motion, and lighting: pseudocoloring is available from a 16 million-color palette; cutting planes can be presented with one palette and opacity table on one side, and an entirely different palette and opacity table on the other side; the transparency feature will reveal details in the data set that would otherwise be hidden. All of these features and many more can be used in self-scripted movie loops.
Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast runs on less expensive hardware.
VoxBlast is available on the Internet for evaluation. Contact VayTek at 515-472-2227 or vaytek-at-ins.infonet.net for download instructions.
xxx
NEWS RELEASE FOR IMMEDIATE RELEASE September 25, 1995
VoxBlastx Now Native for Power Mac
VayTek, Inc.'s software for rendering, measuring and presenting 3D data now runs approximately three times faster in the new, native version for Power Macs.
With this dramatic increase in speed, the Power Mac can match many UNIX workstations in accepting a stack of registered 2D images for creation of 3D projections.
VoxBlast is a cut above most 3D volume visualization programs, offering powerful tools for extracting quantitative information. Arbitrary slicing planes, true 3D measurements, surface extraction, polygon rendering, flood fill capabilities, and volumetric calculations are just a few of the standard features.
The native version of VoxBlast retains the high degree of flexibility and compatibility of previous versions. Data sets from microscope data, CT scans, petroleum engineering data, chip inspection, and other sources are readily accepted, giving researchers a broad range of choices for ancillary equipment.
Researchers at Pioneer Hi-bred in Iowa say, "For the price, Voxblast has no equal." University of Missouri scientists add, "In addition to the high quality images, we were impressed by the flexibility of VayTek's software."
Unlike most other 3D rendering programs, VoxBlast measurements are based on fractional interpolations and careful preservation of original data, so the end results are more accurate.
Sophisticated lighting models, movie generation features, and palette editors are included in the program. Presentaion and evaluation of data is enhanced when the user can incorporate color, transparency, motion, and lighting: pseudocoloring is available from a 16 million-color palette; cutting planes can be presented with one palette and opacity table on one side, and an entirely different palette and opacity table on the other side; the transparency feature will reveal details in the data set that would otherwise be hidden. All of these features and many more can be used in self-scripted movie loops.
Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast runs on less expensive hardware.
VoxBlast is available on the Internet for evaluation. Contact VayTek at 515-472-2227 or vaytek-at-ins.infonet.net for download instructions.
To Jay Jerome, The paper that resulted from the work in Calgary on converting an SEM to a n X-ray microscope is: "X-ray microscopy using a scanning electron microscope for the purpose of imaging central nervous system structures" S. Fletcher, R.S. Hannah and M.J. Hollenberg. Journal of Neuroscience Methods, 7 (1983) 19-25. The microscope that was successfully converted was a Hitachi S-450 and the resulting pictures were like conventional transmission X-rays, but at magnifications of 20X to 2000X.
regards, Mary
Mary Mager Electron Microscopist Metals and Materials Eng, UBC 309 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648, fax: 604-822-3619 email: mager-at-unixg.ubc.ca
Dear All: I understand that the later MACs will read PC-written floppies and programs such as Photoshop and Graphics Converter will read the TIFFs on the PC floppies, in the MAC. I know my Power MAC 6100 running System 7.5 will. Also, we have a Syquest drive that normally resides on the PC, on a SCSI port. That was the tough part. It was very simple to change the SCSI ID on the back of the Syquest and plug it into the Power MAC 6100. The MAC found it and labeled it "DOS" and accessed it fine. Hope this helps. Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Eng, UBC 309 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648, fax: 604-822-3619 email: mager-at-unixg.ubc.ca
Message-Id: {199510130828.JAA00505-at-pons.uio.no} X-Sender: finnmog-at-pons.uio.no X-Mailer: Windows Eudora Version 1.4.3 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Thanks for the answers!
********************************************************* QUERY: Their adress was cited by the Thomas register as Flow Cytometry Standards Corporation 513 Hostos Avenue, Ste. B3 P.O. Box 194344 San Juan, Puerto Rico 00919 but TR could not find any phone/fax numbers.
ANSWERS: 809 753-9341 ( Abe Schwartz )
Finn-Mogens Haug
University of Oslo Institute of basic medical sciences Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no Box 1105 Blindern Phone : +47 22 85 12 67 N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
Nestor and Group - I wonder if I, and perhaps others, may have missed some of the instructions concerning the new server? Do I have it correct: 1) We are to "unsubscribe" from the old server and, I trust, that we all know how to do this. 2) We are then to "subscribe" to the new server? 3) To "subscribe" to the new server, we should follow the rules from the old server - to wit: Send a message to "Listserver-at-MSA.microscopy.com" and then in the text block to do a "subscribe" followed by ones' eMail address. I wonder (and hope) that the above is correct? Regards to all Don Grimes
Dear Microscopists, One of the people in our department asked me if I knew of a dye that they could use to locate the vena cava in rats. They want to disect the vena cava and the aorta and ultimately do EM on the tissue, so the dye can't be particularly nasty. They have been having trouble locating the vena cava. Any ideas out there?
Thaks, Doug ..................................................................... : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212A) P.O. Box 245044 : : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) : :...................................................................:
Message-Id: {199510132209.RAA03724-at-bcm.tmc.edu} X-Sender: joiner-at-bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Dear Microscopists (or maybe more appropriately: Microtomists) -
I have just inherited a used LKB Nova ultramicrotome. However it is missing block holders for 8 mm Beem blocks and the trimming block. If anyone of you have either of these items cluttering up your lab, and you no longer need them or don't want them, I would be very happy to relieve your burden. We are mainly in need of the trimming block. Make me an offer I can't refuse!
Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy Department of Pathology, Rm.286-A Baylor College of Medicine One Baylor Plaza Houston, Texas 77030 U.S.A. tel.: (713)798-4658 FAX: (713)798-3945 joiner-at-bcm.tmc.edu Compuserve: 71555,1206
Hello again, Nestor and group - Thanks to several for their comments re: my last note. I do understand, thanks to Nestor, that one does not have to sign off the old server and sign on the new server - and that the old system will transfer to the new. It seemed, however, that Nestor is recommending that we do so? My question might better be, if one wishes to subscribe (or unsubscribe) on the new server, does he/she follow the old server system and address the requests to "Listserver-at-msa.microscopy.com" - followed by "subscribe" (or unsubscribe), and ones email address? This seems logical, challenged only by the number of folks who are attempting to subscribe, etc. directly on the new server. Sorry to clutter the system - but hope that others, like me, are not sure of the new system. Regards to all, Don Grimes
The budget office has asked us to evaluate the charges in our core facility and we would like to compare them with those of other facilities. Currently, we charge for using the TEM, SEM and Image Analysis Systems and we charge for our technical assistance with particular parts of the experimental procedure(e.g. sectioning). However, the use of the ultramicrotomes, the sputter coater and the CPD are free. So that we might provide our budget office with comparative information, please share with us the charges used in your facility and the cost recovery achieved by those charges. (In our lab the microscopes and technical assistance charges are $15 per hour and the image analysis charges are $6 per hour.) Thanks for your assistance!
Donna Wagahoff SIU School of Medicine P.O. Box 19230 Springfield, Il. 62794-1220 217-782-0898 FAX 217-524-3227
For those few of you that haven't yet heard about it. The good times virus report is a hoax. Ignore it and DO NOT forward it to anyone. A version of that message has been circulating the Internet for nearly two years, with some slight modifications.. It amounts to a chain letter of sorts, just using up bandwidth, and it's only virus capablities are that it continues to resurface in email boxes when a new user finds the message and forwards it around to his/her friends.
You should now send all "Microscopy" mail to the address
Microscopy-at-MSA.Microscopy.Com
You should now send all Subscribe/Unsubscribe mail to the address
Listserver-at-MSA.Microscopy.Com
when you reply to a comment and you wish to allow all subscribers of the Microscopy Listserver to see your reply you should SEND a NEW MAIL message to
Microscopy-at-MSA.Microscopy.Com
if you use the "reply" function of most Email programs your reply will go only to the originator of the message and not to the entire list.
Many people have aliases, shortcuts, nicknames or some other term defined in their Email client programs which allow them to type a short abbreviated phrase to send Email to Microscopy. My request is that all of you change your "nickname" files to use the new address of the listserver.
As a transition from the old site to the new site, I have put an automatic forwarding function on the old site. However, I cannot guarentee that it will be there forever. I did this for 2 reasons, 1.) Many new subscribers find out about the list server from old publications, this allows them to still access the system, in their instructions I will remind them of the new address 2.) Lots of you will forget to change your aliases and then I'll get bunches of mail asking what is wrong if the old address immediately disappears. So it is also my attempt at making my life a bit simplier, however, I never seem to be able to succeed at actually doing that.
} WE are having problems crossing the MAC IBM barrier with TIFF images. Does } anyone know of a simple utility for IBM to convert an IBM-TIFF image file } to otehr formats, MAC readable TIFF at least. } Many thanks } Brendon J. Griffin } Centre for Microscopy and Microanalysis } The University of Western Australia } Nedlands, WA, AUSTRALIA 6907 } ph 61-9-380-2739 fax 61-9-380-1087 }
We also occasionally need to move TIFF files from eg. our Leica TCS4D confocal via a PC to an image analysis package on a Mac Quadra running System 7.1. We use PC Exchange on the Mac to read and write PC format disks, and import TIFF files with Adobe Photoshop, which reads (& writes) both Intel and Motorola (Mac) TIFF formats. The image can then be resaved as a PICT file. Alternatively one can use Apple File Exchange to import TIFF files, but it's slow.
For the PC, to add to the software already mentioned, there's a program called Image Alchemy (for DOS) available as shareware, which converts a huge variety of graphics formats, including Intel and Motorola TIFF. It can convert a PC TIFF file to Macintosh PICT format, which can be read directly into a Mac. The only complication is that the Mac file type should be set to PICT, so that it is recognized as such. With PC Exchange, you can configure it so that a file with the appropriate PC file extension (eg .PCT) is recognized as a PICT file.
Hope it helps. Regards,
Thor Bostrom Analytical EM Facility, QUT Brisbane, Australia
Message-Id: {199510160932.KAA07024-at-atlas.ipc.uni-tuebingen.de} Content-Type: text/plain MIME-Version: 1.0 (NeXT Mail 3.3 v118.2)
I go away for a week and three discussion groups change address!! -- Dr. Keith R. Hallam University of Bristol, Interface Analysis Centre, Oldbury House, 121, St. Michael's Hill, Bristol, BS2 8BS, England Telephone: + 44 (0)117 925 5666 | E-mail: k.r.hallam-at-bristol.ac.uk Facsimile: + 44 (0)117 925 5646 | URL: http://zeus.bris.ac.uk/~phkrh/
I must apologize. There was a major typo on the clarification I sent out late last night. Blame it on just my being tired. All subscribe/unsubscribe messages should go to
Listserver-at-MSA.Microscopy.Com
I've corrected the text below.
Nestor -------------------------
Let me clarify, or at least attempt to do that.
You should now send all "Microscopy" mail to the address
Microscopy-at-MSA.Microscopy.Com
You should now send all Subscribe/Unsubscribe mail to the address
Listserver-at-MSA.Microscopy.Com
when you reply to a comment and you wish to allow all subscribers of the Microscopy Listserver to see your reply you should SEND a NEW MAIL message to
Microscopy-at-MSA.Microscopy.Com
if you use the "reply" function of most Email programs your reply will go only to the originator of the message and not to the entire list.
Many people have aliases, shortcuts, nicknames or some other term defined in their Email client programs which allow them to type a short abbreviated phrase to send Email to Microscopy. My request is that all of you change your "nickname" files to use the new address of the listserver.
As a transition from the old site to the new site, I have put an automatic forwarding function on the old site. However, I cannot guarentee that it will be there forever. I did this for 2 reasons, 1.) Many new subscribers find out about the list server from old publications, this allows them to still access the system, in their instructions I will remind them of the new address 2.) Lots of you will forget to change your aliases and then I'll get bunches of mail asking what is wrong if the old address immediately disappears. So it is also my attempt at making my life a bit simplier, however, I never seem to be able to succeed at actually doing that.
Later... Nestor
Your Friendly Neighborhood SysOp
------------------ RFC822 Header Follows ------------------ Received: by ma160.ms.ornl.gov with SMTP;16 Oct 1995 00:41:55 U Received: from Sparc5.Microscopy.Com by oaunx1.ctd.ornl.GOV (8.6.10/3.0-C) id AAA15144; Mon, 16 Oct 1995 00:41:03 -0400 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id XAA03711 for dist-Microscopy; Sun, 15 Oct 1995 23:05:29 -0500 Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id XAA03708 for {Microscopy-at-sparc5.microscopy.com} ; Sun, 15 Oct 1995 23:05:28 -0500
I (as presumably did others) have just received Princeton Instrument's brochure in the mail. My understanding after talking to them at MSA is that their systems are considerably cheaper than Gatan's, particularly at the 2048x2048 size.
Any comments, pieces of wisdom, experience with using these systems?
I think I keep a pretty good eye on microscopy literature but thought that it would be a good idea to poll the community at large.
What textbooks are being used to teach TEM, particularly at the introductory graduate level for physical scientists? I would like to restrict this line to books currently in print ;)
I have been very impressed by two collaborative texts in SEM and AEM: Goldstein et al's Scanning Electron Microscopy and X-Ray Analysis and Joy et al's Principles of Analytical Electron Microscopy. These books both seem well-written, particularly for collaborative projects, and suitable both for class-use and excellent long-term references. However, I don't know of any book for conventional TEM in print which I regard as highly. Incidentally, Principles of AEM does provide a fine introduction to CTEM but its heart is clearly in the explanation and use of spectroscopy and microdiffraction.
I recall being relatively pleased with a recent book written specifically for mineralogists; someone else must have liked it too since it has walked off my shelf. I would really prefer a more general text for physical scientists though, since I deal with substantial numbers of materials scientists, chemists, and EE's.
Thoughts?
Daniel L. Callahan Department of Mechanical Engg. and Materials Science Rice University dlc-at-owlnet.rice.edu
TENURE TRACK FACULTY POSITION University of Missouri - Columbia
The University of Missouri-Columbia (MU) seeks a tenure-track Assistant, Associate, or full Professor to be jointly appointed in the colleges of Veterinary Medicine and Agriculture, Food, and Natural Resources. The successful candidate will be expected to lead an outstanding independent research program in an area of cell or structural biology of importance to basic biomedical aspects of veterinary medicine and agriculture, teach electron microscopy at the graduate level, and direct (at 20% effort) a recently established, campus-wide Electron Microscopy Core Facility under the auspices of the MU Molecular Biology Program. Experience in both plant and animal systems is preferred, but not required; post-doctoral experience is necessary. Women and members of minority groups are especially encouraged to apply. Applicants should submit, by January 31, 1996, a description of research plans, curriculum vitae, selected reprints and names, e-mail addresses, and telephone/FAX numbers of three professional references to: Dr. Margaret A. Miller, co-chair, Cell Ultrastructure Faculty Search Committee, UMC Veterinary Medical Diagnostic Laboratory, PO Box 6023, Columbia, MO 65205; phone, 314/882-6811; FAX, 314-884-7544; e-mail: miller-at-vmdl.missouri.edu. The University of Missouri is an Affirmative Action/Equal Opportunity Employer.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
Message-Id: {MAILQUEUE-101.951016110004.416-at-vanlab.paprican.ca} To: Davin Jutila {DJUTILA-at-wpsmtp.siumed.edu}
Hi, I have worked in a couple of labs with similar curtains made out of a regular heavy dark fabric, but if I was to do it myself, I would look at the blackout liners available for drapes. Enquire at any local drapery retailer. The reinforced vinyl fabric has the combined advantages of being opaque and non lint producing. Laurie
} Hello all, } I'm new in this area of science and need some assistance: } } We are in need of a 14'x11.5' section of darkroom cloth for seperating } one room with the ability to do light free analysis. We have ran into a } wall with our catalogs and resources in locating a vendor for such a } curtain. Could someone advise us on where we might find such a } vendor or suggest other solution that might have been done in the past. } } Thank you in advance, } Davin } } Davin B. Jutila } Flow Cytometry/Research Imaging Facility } Southern Illinois University School of Medicine } 801 North Rutledge Mail Code - 1220 } Springfield, IL 62794-9230 } Ph#: (217)-782-0898 Fax#: (217)-524-3227 } e-mail: djutila-at-wpsmtp.siumed.edu } }
______________________________________________________________________ Laurie Frederick, A.SC.T. PAPRICAN Corrosion Control Group 3800 Wesbrook Mall The Pulp and Paper Research Vancouver, B.C. Institute of Canada Canada V6S 2L9
I apologize for the message I forwarded to you few days ago regarding to the virus "Good Times". Thanks for all who inform me the truth. I have forwarded Mr. Haug's condensed version of the "Good Times FAQ" to whom I received the mail from. Hopefully, this joke chain letter won't be spread any further.
Best regrads,
Po-Fu Huang
Particle Technology Laboratory (Office) 612-626-7227 Mechanical Engineering Department (Lab) 612-625-7307 University of Minnesota (e-mail) huang020-at-maroon.tc.umn.edu
The LORD is my shepherd. I shall not be in want. -- Psalm 23:1
I have been having troubles sending mail to the new address. This is trying the SPARC5 form. ---------------------------------------------------- Warren E. Straszheim 270 MD Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091 E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
coal characterization and processing electron microscopy, x-ray analysis, image analysis computer applications
} Dear Microscopists (or maybe more appropriately: Microtomists) - } } I have just inherited a used LKB Nova ultramicrotome. However it is missing } block holders for 8 mm Beem blocks and the trimming block. If anyone of you } have either of these items cluttering up your lab, and you no longer need } them or don't want them, I would be very happy to relieve your burden. We } are mainly in need of the trimming block. Make me an offer I can't refuse! } } } Joiner Cartwright, Jr., Ph.D. } Director, Electron Microscopy } Department of Pathology, Rm.286-A } Baylor College of Medicine } One Baylor Plaza } Houston, Texas 77030 U.S.A. } tel.: (713)798-4658 } FAX: (713)798-3945 } joiner-at-bcm.tmc.edu } Compuserve: 71555,1206
I, too, inheirited an LKB 7800 without trimming blocks. So I made one composed of a base (8" by 10" piece of 1" thick plexiglas; drilled and countersunk (from bottom) for bolt), a 2.5" bolt, an ~1.25" spacer made out of metal electrical conduit, a 1/2" drill chuck,m and a couple of big washers. Pass the bolt up through the bottom of the base, add washer, slip the 2 1/2" spacer over the bolt, another washer, then thread the chuck down on the bolt to lock the whole gizmo in place. This setup has full 360o rotation but no tilt. This has not been a problem.
I took the glass base plate out of a dissecting microscope and set the scope over the top of the vertically-pointing drill chuck. Hand-tightening of the drill chuck works fine.
The whole set-up cost about $25--so I made three of them.
Let me know if you need more specific plans. I can draw them up.
I have been having troubles sending mail to the new address. This is trying the MAS alias form. ---------------------------------------------------- Warren E. Straszheim 270 MD Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091 E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
coal characterization and processing electron microscopy, x-ray analysis, image analysis computer applications
************************************************************ Welcome to ************************************************************
Microscopy-at-MSA.Microscopy.Com
************************************************************ Hosted and Sponsored by The Microscopy Society of America (MSA) as a Free Service to the World-Wide Microscopy & Microanalysis Community ************************************************************
G'day Subscribers....... (Well at least I hope it is day-time, when you read this )
It looks as if we are now back on-line, and as the subject title and the above banner indicate some major changes have occurred during the last few days. You might even say we now have a new face, well at least a new envelope !
Firstly, as most of you know I had been running the Microscopy Listserver at ANL for just over 2 years, basically without any real support, save my time and some electricity. As a consequence it was becoming harder and harder to keep things running properly, let alone efficiently.
At this time it is my pleasure to officially announce that the Microscopy Listserver has moved (hopefully, nearly transparently to you but obviously with a bit of work for me) to a new address and with a new sponsor.
MSA at their 1995 Winter/Spring Council meeting, voted to establish a full internet site for the society and it's members and to host the Microscopy Listserver as a FREE service to the microscopy and microanalysis community worldwide. It has taken some time to get everything running, but as of last night, the listserver is now relocated and functioning with a modicum of success. I still basically do the majority of the work (gratis), however, we now have a new workstation (Sun Sparc5), software, and as I mentioned an offical internet site. The server will still need some work, but we now have the resources to improve the system and make it more user-friendly.
For example, with the new configuration of header (i.e. the envelope of this Email message), errors and undeliverables should now no-longer be returned to the originator, but are sent to the SysOp ( Ijust need more mail, right?). In addition, I will be bringing up on-line some of the more usual features characteristic of listserver software in the coming weeks . I will post the information to to the list as the options become functional.
I would encourage you to send thanks to MSA Council for saving the listserver from a slow death, as the hardware and software configurations at ANL were becoming increasing difficult to maintain and as some of you know were beginning to get particuliarly ornery. } From my own perspective, my thanks go to all the members of council for establishing this site, and a special thanks to two members who have been especially supportive namely- Ron Gronsky of UC Berkeley and Ron Anderson of IBM.
The general MSA Email Address is:
MSA-at-MSA.Microscopy.Com
while the business office can be reached at:
MSABusinessOffice-at-MSA.Microscopy.Com.
Additional information about MSA, the society and it's operations can be found at the MSA WWW site:
http://WWW.MSA.Microscopy.Com
MSA also mirrors the MMSLIB (Microscopy & Microanalysis Software Library) anonymous ftp site at:
FTP.MSA.Microscopy.Com
and as you already know the Microscopy Listserver at:
Microscopy-at-MSA.Microscopy.Com
Mail from the old server site (Microscopy-at-AAEM.AMC.ANL.GOV) will be automatically forwarded to the new server site for awhile, but please change your local aliases and/or shortcuts, so that your mail goes directly to the MSA site instead of channeling through ANL.
As I mentioned, there will still be a few growing pains, but the hard part should be basically done.
In addition to the move from the ANL to the MSA site, the listserver has passed another milestone this weekend, the 3,000th subscription request arrived, not bad for only 2 years of operation. The lucky winner of a beer should he and I ever meet is:
Russell J. Wilson Analytical Electron Microscope Facility Queensland University of Technology Garden Point G.P.O Box 2434 Brisbane Qld. 4001.
Russell, it's your job to make sure that a cool slab of tinney's is around, but I'll buy!
BTW if you are a triva buff or just curious the list sends Email to Microscopists in 39 countries around the world. Over 5600 Email messages have been submitted to Microscopy over the period Oct 1, 1993 to Oct 1, 1995. Not all of them have been gems, but there have been occasional useful bits of info......
I have been asked to find out how to prepare powder samples for microprobe alaysis and I wondered if anyone out there in Microland did this on a routine basis.
Any pointers would be most helpful.
I have had previous samples made into pellets using a press which was used to prepare IR spectroscopy discs but the results are rather disappointingly bumpy.
Thanks in advance,
Robert McDonald & Cameca SX50 Dept of Geology & Applied Geology Glasgow University Glasgow SCotland
} I have been having troubles sending mail to the new address. This is trying } the MAS alias form. ---- Sorry for the inconvenience my two posting may have caused. Nestor picked up on the subtle problem. I had been sending my mail to MAS.Microscopy.Com as hinted above. Strange enough, I got the address right in the header of my test message even though I referred to _MAS_ in the body. Thanks to Nestor for pointing out my dyslexia. I checked through my past attempts to post and saw that the MAS error was indeed the culprit. It just took more than a week to figure that out.
By the way, I never found the typo that Nestor fixed the other day in his Typo in Clarification message. But all is well now, right? ---------------------------------------------------- Warren E. Straszheim 270 MD Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091 E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
coal characterization and processing electron microscopy, x-ray analysis, image analysis computer applications
We are reorganizing our sample preparation lab and in the interest of eliminating duplicative equipment/capabilities, we have two items to offer for sale. The first is a dimpler/grinder (for 3mm samples) including accessories. Secondly, we have an ultrasonic 3mm disc cutter. Both instruments are in nearly new condition and the first reasonable offer received will be accepted. You may contact me, or preferrably, Dr. Farhad Shaapur, Center for Solid State Science, (602) 965-0399, or shaapur-at-csss.la.asu.edu for further specifics.
I do not have experience in analyzing powder samples, here are just some technical thoughts (assume you are considering WDS detectors). A few things should be considered in EPMA analysis:
1. the sample has to stand for the electron beam without any physical and chemical change during analysis. 2. the sample need an ideal geometry, a smooth flatten surface in most cases, for matrix and other corrections in analyzing. 3. for the same reason you also need standards that have similar structure and composition as the sample. I think the IR spectroscopy disc-type sample is not strong enough to hold the particles during the analysis. (Also there are better ways to analyze powder samples, such as XRF, ICP-AES(or MS), AA, and wet chemical method. The point to use EPMA is for analyzing the micro scale phases or particles that are difficult to be extracted from the whole sample).
Xiaogang
**************************************** * Xiaogang Xie * * Department of Geology and Geophysics * * Louisiana State University * * Baton Rouge, LA 70803 * * Office (504)388-2240 * * Fax (504)388-2302 * ****************************************
COMBINED CENTRAL STATES MICROSCOPY SOCIETY AND MISSOURI-ILLINOIS-KANSAS MICROBEAM ANALYSIS SOCIETY MEETING
WHEN: ON NOVEMBER 10, 1995
WHERE: JOE HANON'S RESTAURANT, 2430 Old Dorsett Drive (just off of I-270 and Dorsett exit) IN ST LOUIS, MO..
SPEAKERS: Jon McCarthy (MIKMAS Sponsored Speaker) will discuss recent advances in the area of EDS. CSMS Sponsored Spears include: Nestor Zaluzec (that's right, the one and only) who will talk about telepresence microscopy and recent advances in digital communication of microscopical images. Susan Wente (Washington University, St. Louis) will present a discussion of epitope labeling, a new technique for molecular localization in the biological sciences.
TIME: MIKMAS will have the morning slot and CSMS the afternoon slot.
MORE INFORMATION: Detailed maps and a more developed program will be mailed out in one week to interested parties.
CONTACT: John Bozzola at above e-mail address.
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
I am using a Nikon Microflex UFX camera setup on a light microscope with Hoffman modulation. I have a 4x5 back and am wanting to purchace a 35mm "Dark Box" FX 35-A or FX35-WA. These are obsolete and I have struck out with the ususal sources. Anyone with one for sale respond directly to me. Larry Albright 419 Sunset Avenue Venice,CA 90291 Phone 310-399-0865 albrite-at-netcom.com albrite-at-leonardo.net. Thanks! Larry Albright
I am using a Nikon UFX electronic camera on a Light microscope. I only have the 4X5 back and am looking for a film holding back for 35mm work. The model is FX35-A or FX35-WA, either will work with my system. Anyone with one to sell please contact me direct. Any help will be appreciated. Larry Albright
Dear Robert: I have prepared powder samples for the EPMA many times, but have never found a truly acceptable way. Usually I mix the powder with epoxy and make a one inch button, then polish and carbon-coat it. This is OK for larger particles and dense materials, but particles smaller than the beam-interaction volume will give inaccurate quant. results. If you can achieve good density with your press, use the lowest magnification compatible with your spectrometers to smooth out surface roughness effects. Preparing a set of well-characterized standards by the same method will help.
Best of luck, Mary
Mary Mager Electron Microscopist Metals and Materials Eng, UBC 309 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648, fax: 604-822-3619 email: mager-at-unixg.ubc.ca
Thanks to all who replied to my original query of October 10, it seems that the overwhelming consensus is that if one wants to avoid Si contamination of specimens (as I do, not all minerals contain Si), one should avoid silicones. Most people seem to use Santovac 5. So on to the inevitable next question, which is: How the heck can I clean out the system in preparation for changing to Santovac? One user suggested heating it (off the system) with dishwash detergent solution, but I'm pessimistic about the chances of this removing silicone oil and sludge. I've heard a rumour of a suitable solvent, but no details.
Has anybody got a good way?
I have to have my battle plan ready before I take the coater out of service, as I have only one coater, can't afford to be off line for very long. Santovac is so expensive down here that I don't want to contaminate the new oil.
thanks
Ritchie
Ritchie Sims phone: 61 9 3737599 ext 7713 Department of Geology fax: 61 9 3737435 University of Auckland Private Bag 92019 Auckland New Zealand
Thanks to all who replied to my original query of October 10, it seems that the overwhelming consensus is that if one wants to avoid Si contamination of specimens (as I do, not all minerals contain Si), one should avoid silicones. Most people seem to use Santovac 5. So on to the inevitable next question, which is: How the heck can I clean out the system in preparation for changing to Santovac? One user suggested heating it (off the system) with dishwash detergent solution, but I'm pessimistic about the chances of this removing silicone oil and sludge. I've heard a rumour of a suitable solvent, but no details.
Has anybody got a good way?
I have to have my battle plan ready before I take the coater out of service, as I have only one coater, can't afford to be off line for very long. Santovac is so expensive down here that I don't want to contaminate the new oil.
thanks
Ritchie
Ritchie Sims phone: 61 9 3737599 ext 7713 Department of Geology fax: 61 9 3737435 University of Auckland Private Bag 92019 Auckland New Zealand
} Date sent: Tue, 17 Oct 1995 13:44:03 +0100 } From: Robert McDonald {robert-at-geology.gla.ac.uk} } Subject: EPMA powder sample prep } To: Microscopy-at-Sparc5.Microscopy.Com
} } I have been asked to find out how to prepare powder } samples for microprobe alaysis and I wondered if anyone } out there in Microland did this on a routine basis. } } Any pointers would be most helpful. } } I have had previous samples made into pellets using a } press which was used to prepare IR spectroscopy discs } but the results are rather disappointingly bumpy. } } Thanks in advance, } } Robert McDonald & Cameca SX50 } Dept of Geology & Applied Geology } Glasgow University } Glasgow } SCotland } } robert-at-geology.gla.ac.uk
My understanding is that simply pressing powders is very unlikely to produce the smooth horizontal surfaces neccessary for decent results. I have occasionally managed to embed dusts down to about 50 microns in epoxy resin in a mould, followed by the usual geological grinding and polishing, and I'm on the verge of ordering some Spurr's resin (used in biological fields, A R Spurr, J. Ultrastruct. Res., 26,31-42 (1969)) which is supposed to be very low viscosity. I'll be interested in the other replies.
cheers
Ritchie Ritchie Sims phone: 61 9 3737599 ext 7713 Department of Geology fax: 61 9 3737435 University of Auckland Private Bag 92019 Auckland New Zealand
To all in Cyber-microland : Does anyone have experience /data on lifetime and operational quality of Kevex EDX detectors which do not use LN2 ? cheers jjm
Professor John J. Millar Head, Department of Applied Physics Electron Microscope Unit RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001 +613 660 2602 fax 613 660 3837 email jjmill-at-rmit.edu.au
Message-Id: {199510181250.NAA29438-at-pons.uio.no} X-Sender: finnmog-at-pons.uio.no X-Mailer: Windows Eudora Version 1.4.3 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
To the query by Laurie Marks on Princeton Instruments cameras for TEM (I have lost the original message). No pieces of wisdom, I am sorry, but a question.
What is Princeton's offer for TEM-use? One of their TE-series cameras, or the Pentamax, with adapters for which TEM's? At what prices?? Do they offer their standard software/is it adapted for use with TEMs?
For the last 5 years we have recorded digital images with a video-format Gatan 673 MkII "Wide angle camera" from our Philips CM10. We are curious about high-resolution cameras.
Thanks for any information, regards
Finn-Mogens Haug
University of Oslo Institute of basic medical sciences Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no Box 1105 Blindern Phone : +47 22 85 12 67 N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
What is the best microtome to purchase? I have used and enjoyed an Ultracut E. However, they are no longer being made. Leica now has a motorized/digital Ultracut S which I currently use. Although it is a nice machine, it is difficult to face quickly with a glass knife due to the greater resistance of the wheel. (my samples will fracture with razorblade facing) Another facility has asked for our input on a microtome purchase. They are considering a RMC MT-7000. Is this a good model? Are there any used ultracut Es for sale? I look forward to hearing your views and suggestions. Shelley Landon Kaurin
Thanks to all who offered suggestions for my problem regarding removing Dow Corning silicone HV grease from our Gatan double-tilt holder. After talking more than once with Dow Corning, as well as with technical support at Gatan, I am please to report that solvents for this grease do exist. They are either: methyl ethyl ketone, mineral spirits, methyl isobutyl ketone, or a commercial solvent from Amtex Chemical in West Chester PA (610-436-4813). Of these I tried methyl isobutyl ketone on grease smeared on a glass slide and this worked in fairly short order. Jeff Gronsky at Gatan reported good success using mineral spirits in an ultrsonic with later cleaning in freon. So that, as they say, is that.
Roy Christoffersen Texas Center for Superconductivity 3201 Cullen Houston, TX 77204-5932 roy-at-bayou.uh.edu (713) 743-8273 FAX: (713) 743-2787
I think that pricing of CCD's as with everything, is a question of negotiation. For that you will have to contact Princeton directly (although I will do it for anyone for a commission!)
Shelley- I share your opinion about the Ultracut E, it was the best ever built. The simplicity, reliability and dependability of these machines was much like the VW Bug, which was also discontinued (for a while) due it's long lifetime. I would pursue the used Ultracut E. Good luck! -Mike
On Wed, 18 Oct 1995 kaurin-at-rmslab.rockefeller.edu wrote:
} What is the best microtome to purchase? I have used and enjoyed } an Ultracut E. However, they are no longer being made. Leica now has a } motorized/digital Ultracut S which I currently use. Although it is a nice } machine, it is difficult to face quickly with a glass knife due to the greater } resistance of the wheel. (my samples will fracture with razorblade facing) } Another facility has asked for our input on a microtome purchase. They are } considering a RMC MT-7000. Is this a good model? Are there any used ultracut } Es for sale? I look forward to hearing your views and suggestions. } Shelley Landon Kaurin } } }
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
There has been some recent discussion on the subject of mounting powder samples for EPMA. I would like to present an alternative approach, one in fact we believe to be superior, that being through the use of our own "Tacky Dot Slide" product family.
No point in making a commercial here, you can obtain a full description of SPI's Tacky Dot slides directly from the SPI Supplies WWW site given below. From the "home page", just "click" on "new products" and then "click" on "Tacky Dot" slides.
For EPMA preparation, what is needed is called the "Circle Analytical Mount" holder which permits one to mount (in your favorite plastic) the particles in a perfect orthogonal array, and then after polishing down to cross sections, and if the EPMA system being used has stage automation, then the analysis can be done completely automatically, without human intervention.
The use of this system permits more accurate data that can be obtained in a far shorter period of time.
You also have to select a "Tacky Dot" slide with a dot size that would be appropriate for the particle size at hand. Generally speaking the method is appropriate for particles larger than about 6-8 um and not larger than about 1000um. There are currently eight different dot sizes from which to select.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
Please, remove my name from the list. I wasn't aware that I would get so many messages, which are really of no use to me. I think the idea is great, but we need still some time so that messages can better allocated to the appropriate person. Thanks for courtesy of removing my address,
} On Wed, 18 Oct 1995, I wrote: } } } To all in Cyber-microland : } } Does anyone have experience /data on lifetime and operational quality } } of Kevex EDX detectors which do not use LN2 ?
I am happy to collect and post summary of responses. Thanks to all private replies requesting same. Cheers jjm Professor John J. Millar Head, Department of Applied Physics Electron Microscope Unit RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001 +613 660 2602 fax 613 660 3837 email jjmill-at-rmit.edu.au
As far as I know, the procedure of analysis of powder samples is same as that of analysis of microparticle samples. Dr. John Armstrong of CalTech has published numerous papers about EPMA quantitative elemental analysis of microparticles. He also designed a particle analysis program. One of his articles "Quantitative elemental analysis of individual microparticles with electron beam instruments" is in the book ELECTRON PROBE QUANTITATION, edited by Heinrich and Newbury (1991).
Good luck!
Long Liang ARCO EPMA/SEM Lab Plano TX _______________________________________________________________________________
} Date sent: Tue, 17 Oct 1995 13:44:03 +0100 } From: Robert McDonald {robert-at-geology.gla.ac.uk} } Subject: EPMA powder sample prep } To: Microscopy-at-Sparc5.Microscopy.Com
} } I have been asked to find out how to prepare powder } samples for microprobe alaysis and I wondered if anyone } out there in Microland did this on a routine basis. } } Any pointers would be most helpful. } } I have had previous samples made into pellets using a } press which was used to prepare IR spectroscopy discs } but the results are rather disappointingly bumpy. } } Thanks in advance, } } Robert McDonald & Cameca SX50 } Dept of Geology & Applied Geology } Glasgow University } Glasgow } SCotland } } robert-at-geology.gla.ac.uk
With regard to microtomes, I learned along time ago that one persons diamond is another's lump of carbon. I personally love the Ultracut E and wish it was still available. However, I would suggest getting each of the microtome companies to let you try their microtome for a couple of days on your own material before purchasing. Everyone makes a good microtome, it is a matter of individual taste which you prefer.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
On Wed, 18 Oct 1995 kaurin-at-rmslab.rockefeller.edu wrote:
} What is the best microtome to purchase? I have used and enjoyed } an Ultracut E. However, they are no longer being made. Leica now has a } motorized/digital Ultracut S which I currently use. Although it is a nice } machine, it is difficult to face quickly with a glass knife due to the greater } resistance of the wheel. (my samples will fracture with razorblade facing) } Another facility has asked for our input on a microtome purchase. They are } considering a RMC MT-7000. Is this a good model? Are there any used ultracut } Es for sale? I look forward to hearing your views and suggestions. } Shelley Landon Kaurin } } }
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Speaking of Princeton Instruments, I just had a demo of their "Micromax" cooled CCD digital acquisition system a couple of days ago.
It took all of fifteen or twenty minutes to vent, bolt on, and pump down the 35mm port version on my Philips 400T, and start collecting images. Considering most of the demos we've had here, I was darned impressed. Oh, yeah, the image quality was excellent as well.
I tested the YAG scintillator type rather than the phosphor. The system mates Princeton's camera system with a microscope interface furnished to them by Fullam. I'll let them do the selling job on the "advanced" features, but I will recommend giving them a look.
The cost on my quote is comparable with their only competitor's (that I know of) in this price range (AMT), namely in the $60K Can. range.
I'm still trying to arrange a demo of AMT's offering to make my final selection, but I'm convinced by what I've seen so far that this type of system will certainly be suitable for our routine TEM imaging needs (i.e materials BF, DF, SADPs).
Princeton Instruments Canada, BTW is at (613) 836 1074 (FAX) or 76261.2210-at-compuserve.com
AMT is at (508)948-5507 (FAX)
If I've missed another candidate, I'd appreciate someone letting me know.
############################################################### # # # Don Steele STEELE-at-KRDC.INT.ALCAN.CA # # ALCAN INTERNATIONAL # # Kingston Research and Development Center # # P. O. Box 8400 # # Kingston, Ontario Canada K7L 5L9 # # # ###############################################################
Message-Id: {9510190338.AA24661-at-gamgee.cc.flinders.edu.au} Comments: Authenticated sender is {mnklg-at-[129.96.250.33]}
} Thanks to all who replied to my original query of October 10, it } seems that the overwhelming consensus is that if one wants to avoid } Si contamination of specimens (as I do, not all minerals contain Si), } one should avoid silicones. Most people seem to use Santovac 5. } So on to the inevitable next question, which is: } How the heck can I clean out the system in preparation for } changing to Santovac? } One user suggested heating it (off the system) with dishwash } detergent solution, but I'm pessimistic about the chances of this } removing silicone oil and sludge. I've heard a rumour of a suitable } solvent, but no details. } } Has anybody got a good way? } } I have to have my battle plan ready before I take the coater out of } service, as I have only one coater, can't afford to be off line for } very long. Santovac is so expensive down here that I don't want to } contaminate the new oil. } The "official" method of cleaning diffusion pumps in anticipation of using Santovac 5 was supplied to me by Edwards Australian Agents ten years ago. The procedure is " 1. empty the old oil charge. 2. block the outlet. 3. Fill the pump with a charge of 'Genklene' 4. Put glass plate on the top flange to seal. 5. Switch on the heaters and continue till the vapour reaches the top glass plate. 6. Turn on the cooling water and turn off the heaters. 7. Wait till the pump cools before opening 8. repeat. 9.pour out the Genklene and recharge with santovac 5.
for any degreasing use carbon tet. Due to the highly toxic fumes produced when carbon tetrachloride is heated, Genklene is recommended as a safer cleaning fluid in the pump boiler."
Kerry Gascoigne Flinders Microscopy and Image Analysis Facility
Message-Id: {9510190612.AA27002-at-gamgee.cc.flinders.edu.au} Comments: Authenticated sender is {mnklg-at-[129.96.250.33]}
for any degreasing use carbon tet. Due to the highly toxic fumes produced when carbon tetrachloride is heated, Genklene is recommended as a safer cleaning fluid in the pump boiler."
Kerry Gascoigne Flinders Microscopy and Image Analysis Facility
In a previous message about PC {-} Mac image file conversion, I mentioned a PC program called Image Alchemy, which is able to convert some 70! graphics file formats. Sorry I didn't give a source site for it (I found the demo version on a Shareware CD-ROM), so here it is.
Image Alchemy is produced by Handmade Software, Inc., who have a web site at http://www.handmadesw.com/hsi/products.html which also gives ordering info. A demo version can be downloaded from ftp://ftp.handmadesw.com/pub/alchemy/ (the MSDOS version file is alch18.zip 677Kb)
To avoid confusion, I should mention that there's also a company called Alchemy Mindworks Inc. who produce the well-known Graphic Workshop and other graphics software. Their web page is at http://www.north.net/alchemy/alchemy.html and they give FTP sites for their shareware as uunorth.north.net (directory /pub/alchemy) and FTP mirror sites at ftp.rose.com and www.visi.com
I have no connection with these companies, just tried some of the software.
Thor Bostrom Analytical EM Facility, QUT Brisbane, Australia
(PS: Because of a problem with our university mail server I haven't been receiving any Microscopy mail recently. I'm sending this out into the void.)
OK, OK I get the message that I sent my subscribe message to the wrong address. About a dozen people have sent messages! I would have thought that the SysOp would be the only one to send a message. Sending to the correct address now............. Please do not send out any more wrong address messages! L.Sadowski
I use an Ultra-cut S and have found it to be a very reliable machine. I do not understand your comment about it taking to long to face a block (what kind of block?) due to the resistance of the wheel. What does this mean? If your samples fracture with razor blade facing, than why would microtome facing be worse?, I would think it would be better because you con control not only thickness of facing cuts but also speed. I have no experience with the RMC so no comment on them.
Best of Luck, Ed Calomeni Medical College of Ohio Toledo, OH emlab-at-opus.mco.edu
We are interested in purchasing a film recorder to output confocal images and SEM images on to film. I would like to get feedback from the group about their experiences with high resolution recorders. In particular, it seems that some of the film recorders do well with graphics (text, graphs, etc.) but distorts images.
I have posted this question to the confocal listserver and only got one reply. So far, it looks like the Polaroid and Mirus recorders are not acceptable.
Thanks in advance for any comments and suggestions.--
Many years ago, we were asked by JEOL to design adapters to convert their SEMs from 4 x 5 Polaroids to 35mm. We had a number of these made over the years, for models ranging from the JEOL 35 to the 840 series. Before I convert them into very snazzy black lucite birdhouses, is there anyone out there who would like to buy one (cheap!)? Steven Slap, Vice-President Energy Beam Sciences, Inc.
Many years ago, we were asked by JEOL to design adapters to convert their SEMs from 4 x 5 Polaroids to 35mm. We had a number of these made over the years, for models ranging from the JEOL 35 to the 840 series. Before I convert them into very snazzy black lucite birdhouses, is there anyone out there who would like to buy one (cheap!)? Steven Slap, Vice-President Energy Beam Sciences, Inc.
Madison Area Technical College (Madison, Wisconsin) has openings for one or more part-time EM instructors. Contact MATC Human Resources Office, PO Box 7128, Madison, WI 53707-7128 (608/246-6900) for application forms and Affirmative Action forms.
DUE DATE FOR RECEIPT OF APPLICATION MATERIALS IS NOV 3, 1995.
The one I like is "Grpahic Converter" It is by Thorsten Lemke. Lemke Software Insterberger Str. 6 31228 Peine Germany 100102.1304-at-compuserve.com
This converts almost every file format I know to everyother format. Has some glitches, but is a really nice program.
The version I have is 2.15. It is shareware and you can FAX the order form to him and give a credit card number. I have registered (havent heard from him yet tho').
John Mansfield North Campus Electron Microbeam Analysis Laboratory 413 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (313)936-3352 FAX (313)936-3352 jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu or jfmjfm-at-umich.edu they all reach me! URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html
I too am interested in doing very similar things. The name i have ceom up with is Laser Graphics LFR (Lasergraphics Film Recorder) at 714-753-8282. Their options are:
LRF - Personal Plus - Permanantly mounted 35 mm camera back - 33-bit color depth output (sorry I don't know the max resolution) - $6,000 US
LFR X-95 - Three switchable cammera backs: 35mm Standard, 35mm Bulk film, and polaroid 4x5. - 33-bit color depth - $7,000 US
LFR Mark II - Standard 35mm, 35mm bulk film, polaroid, 120, 220, or 4x5 - 36-bit color depth - $10,000 US
LFR MARK III - Standard 35mm, 35mm bulk film, polaroid, 120, 220, or 4x5 - 36-bit color depth upto 8k x 8k resolution - $20,000 US
I know that these systems are designed for DOS/Windows PC systems, I do not know if other systems are also possible.
} ......... Most people seem to use Santovac 5. } So on to the inevitable next question, which is: } How the heck can I clean out the system in preparation for } changing to Santovac? } One user suggested heating it (off the system) with dishwash } detergent solution, but I'm pessimistic about the chances of this } removing silicone oil and sludge. I've heard a rumour of a suitable } solvent, but no details.
Richie: When I clean out my vacuum evaporator (about every 3-4 years) after washing off "wet" oil coating from the diffusion pump casing and stage stack(disassembled) with hot detergent solution as described above followed by acetone rinse, I then take the dried parts - diff pump casing, stack parts - over to my campus Scientific Apparatus shop where they use a glass bead blaster to remove the burned on dark deposits that are virtually impossible to remove with detergents or by scrubbing. It works beautifully, the bead stream quickly "melts" the deposits off leaving a fresh clean metal surface that your new oil charge will just love to boil up against!
Try to find a shop with a bead blaster. You may have to go to your local auto body shop if your facility has none available. Hope this helps. Gib
--
Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
I came from a imaging core that had two RMC MT7s and a Reichert Ultracut S to a facility with only an RMC MT6000XL. At my former workplace, the difference was night and day -- the Reichert was a beautiful instrument with no troubles experinced at all (except the under-lit bulb tends to burn out often unless you replace it with the longer life filaments.) The RMC, however, was, to put it gently, a piece of garbage. Constant problems with the RMC service taking much too long, and the microtome simply did not seem well built. At one point, the microtome was out of service for about month while waiting for the engineer to show up. When he finally did, it took another week or two to fix the problem. I can elaborate further on the problems we had, but I think we just bought a lemon.
On the other hand, I have since been cutting on the MT6000XL, and it has been *very* nice. I have no complaints about the microtome, although hopefully it will not be in need of service anytime soon.
Other comments are that the RMC feels more like a "traditional" microtome with the large wheel, whereas the Reichert has a very small wheel that takes some getting used to. Also, all the controls for the RMC are within the console while the Reichert has them on a separate unit. The overhead flourescent light on the Reichert is fixed in place, which can be both good and bad. Good because the light on your thins is always consistent; bad because sometimes you want to move it depending on the placement of your diamond knife.
Regardless, before you make your decision, I would definitely see if you can use each product on a "loaner" basis just to get a feel for each.
MR-Received: by mta RVAX.MUAS; Relayed; Thu, 19 Oct 1995 12:26:12 -0800 MR-Received: by mta MEDEC; Relayed; Thu, 19 Oct 1995 12:26:13 -0800 MR-Received: by mta CHUB1; Relayed; Thu, 19 Oct 1995 12:25:49 -0800 Disclose-recipients: prohibited
I have use two film recorders the Polaroid Digital palette and the Focus Graphics analog Imagecorder.
The Polaroid has been a disapoinment. Small lines and text tend to dissapear in the slide. Also, we have had problems with washing out of portions of the slide because some color combinations don't work well together. This seems to be a problem of the small CRT used in the system.
The Polaroid system also requires a third party driver. Most of the problems with the driver seem to be resolved now, but there have been headaches with this also. The problem arises with objects that the driver can't convert. It also takes from 3-5 minutes for printing each slide.
Recently analog (splits the signal going to the monitor) slide makers that work at the PC screen frequency have become available. Previously they only worker with high end work stations .
The system we have has worked very well on our Pentium system. The resolution is excellent, anything displayed on the monitor shows up on the slide exactly the same, and it takes only a few seconds to print each slide. There is a slight jagged look to the text compared to the Post Script Polaroid system. (We have printed graphics, scanned images, gels, etc.)
David Leaffer Roche Bioscience david.leaffer-at-syntex.com
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In response to Philippe Buffat's comments, I WAS concerned about damage to the scintillator in collecting SADPs. The patterns I collected were with the beam stop in place. In the salesman's place, I likely would have requested that that particular acquisition type not be tested. Admitedly, I was taking advantage of what was likely inexperience with TEM on the part of the salesperson.
The benefit of collecting some patterns this way are that you can use the 12 bits of range in the image (in the case of the camera I was testing)to see faint spots which can be difficult to image and record (superlattice spots and the like).
Our facility is a multiuser lab, but with limited, trained users. I figure that the speed, dynamic range, ease of data sharing benefits will outweigh the risk of damage.
The manufacturer is certainly not going to warranty scintillator life, but then it won't take very many fried scintillators for me to outlaw SADP collection on a system. The plate film camera will always be available for that anyway.
} From: IN%"philippe.buffat-at-cime.uhd.epfl.ch" 19-OCT-1995 13:44 } To: IN%"STEELE-at-KRDC.INT.ALCAN.CA" } Hello, } } Don Steele said that he had good test for recording BF,DF and SADPs with a } cooled CCD camera (YAG screen). } } I am glad to hear this. We are many to think that we should stop with } photographic films for many applications. But I am also really surprised. } } To my knowledge, until now, no slow scan CCD supplier has accepted to } warranty the YAG life when it is used for SADPs. } } They all say that you can record SADPs only if you properly hide the (000) } transmitted beam with a beam stop or if this beam IS NOT TOO BRIGHT (!??). } They also say that when this beam hits the YAG target, it will } irreversibly damage (decompose) it within about a few tenths of a second if } the sample is thin and the pattern well focused. } At least in a multi-user environment, nobody can exclude that somebody } forgets to put the beam stop or that the (000) moves out of it } (charging/magnetic effect). Who is really ready to take the risk? } } Is Micromax a more resistant camera/screen than competitors, or is } Princeton Instruments not aware of the risk, or are the others competitors } too cautious? } } Moreover, if you record a SADP with bright difracted spots, you may see a } memory (remanence) effect on the next DF images. Gatan told me last week } that you can get read of this memory effect by flooding the YAG with a } uniform BRIGHT illumination for about 10 s after each diffraction pattern. } No doubt it works fine for the camera, but would you consider that these } operating conditions are the best for DF (LOW light level) observation (and } the operator's eyes health. } __________________________________________________________________ } Philippe Buffat Ecole Polytechnique Federale de } Lausanne (EPFL) } Centre Interdepartemental } de Microscopie Electronique } Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland } Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time) } E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch } ______________________________ Eudora F2.1 ___________________________ }
A vapor degreaser will do a nice job of cleaning a diffusion pump provided that the DP will fit into the degreaser. First disassemble the DP, remove the stack and clean with acetone prior to using the vapor degreaser. Dissassemble the stack unless it is a one piece type and also clean it with acetone. The stack is usually the hardest to clean and you might consider bead blasting followed by a thourough polishing to remove the Si bead residue that might become embedded into the softer stack metal from the bead blaster.
The roughing and backing lines can be cleaned by pulling saturated cloths through the lines with multi conductor electrical wire of 14-18 ga. This may need to be repeated several times starting with petroleum ether and a final cleaning of acetone.
The roughing and backing valves should be disassembled and thouroughly cleaned with petroleum ether and acetone. This cleaning and overhaul is also a good time to replace all of the "O" rings with viton seals.
The vacuum chamber should be cleaned and if the system has a LN2 baffle or trap this should also be cleaned with petroleum ether and acetone.
Excercise patience, as this can be a messy task but it will be worth your effort. Have adequate ventilation and avoid breathing vapors by using the acetone and petroleum as sparingly as possible.
Hope this helps.
John Humenansky Advanced Research Corp. 612-331-2211
We have an old ZEISS 10A TEM (serial # 4684) for sale. This scope is still used, in excellent working condition (considering its age), and has always been on service contract. Nothing special, not even a goniometer holder, but it is well stocked with spare parts. We need the space for a new microscope, therefore in about a month this scope will be de-installed and put into storage. Asking price is $5000 or best offer, this includes the pumps and the water chiller. For further information contact me. Sincerely, Laszlo G. Komuves, PhD Director, Morphology Laboratory Department of Dermatology University of California San Francisco, and VA Medical Center Phone: (415)221-4810, Ext.3720 Fax: (415)751-3927
Having had a Presentation Technologies FR1 for more than five years, I have found that the output from video sources to this unit is adequate for projection slides but not acceptable for negatives. On an opposite note positives and negatives generated from a computer are quite acceptable. Tis unit is extremely slow but very good resolution. The new FR2 model is faster but not as sharp for some unknown reason.
Reviewing the currrent market for a unit which will output at peak resolution (2K, 4K, 8K) for both positives and negatives in multiple formats ( 35mm to 120 to bulk) the OPAL from MGI of Minneapolis ( 612 854 1222) is far and away the winner. This unit comes with a hefty price , around $16K with software. If price is no object, this is the best in the medium range. Others in the commercial range, above $25K, do offer faster print times, higher line scan rates, larger bulk loaders.
The recorders from AGFA are all acceptable, albeit very slow, and not as user friendly, in the under $10K price range. The medium price range ($15K+) is even less user friendly to the occasional operator.
The Lasergraphics units do have a nice positive output, but fall down on the negative output. This is the most often purchased unit in this area for making slides for presentation as of this date.
Polaroid is amateur material for the non-microscopist market.
Regards , melsen-at-MICROBIO.emory.edu L.R. Melsen Emory University Microbiology and Immunology 3029 Rollin Research Center Atlanta, Ga 30322 404 727 3508 FAX 404 727 3659
Results DOS-MAC Syquest: Thanks to all those who responded to my inquiry. As you remember the problem was not translating files but rather seeing (mounting) a DOS formatted Syquest on a Mac desk top. The good news is that I did not have to buy anything else. The Dos Mounter multiMounter software can do it with no problem. However, the newer version of PC access on the upgrade of Mac system 7.5 does it as well. The older version (first release does not). Those of you using McLink Plus, should know that if you upgraded from a system Mac system 6 to a 7 version, the old driver (sticky) will put its signature on MOST DOS mounted media adding a MacLink start up document...When this happens you can not just double chick on those document to start the application that created them, because you may be up for a surprise on what comes up on the screen. On translation, yes I use for while Graphic converter. It is a Shareware (pay your dues) and can be downloaded from http://hyperarchive.lcs.mit.edu/HyperArchive/Archive/gst/grf/graphic-converter-215.hqx, but make sure you have an installed version of Stuff or the like to convert the binhex file into something you can use. Also someone suggested getting the program Alchemy from http://www.handmadesw.com/hsi/products.html, but I have not downloaded yet. If you have any comments or questions please address them to my directly rather than to the list. ****************************************************************** *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services * ******************************************************************
Here at Tulane/Pathology we used a MONTAGE (From Present. Techn. in Ca 408-730-3700) film recorder (DOS-MAC) for years with almost no problem. The resolution is 4000lines (same as commercial labs. It takes time to learn a few hard to learn tricks (at least in the older model), but the techn assistance has been good. We literally recorded hundred of rolls with no problems. Two problems deserve mention: a) If you use Power Point to makes slides and fuse different styles, you could end up with a color background you did not choose, b) The default size does not film the frame of a 35 mm film. Thus, it has to be manually changed! I am not associated with the company that makes the product and gives this information as a user's advice. ****************************************************************** *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services * ****************************************************************** _______________________________________________________________________________
I have use two film recorders the Polaroid Digital palette and the Focus Graphics analog Imagecorder.
The Polaroid has been a disapoinment. Small lines and text tend to dissapear in the slide. Also, we have had problems with washing out of portions of the slide because some color combinations don't work well together. This seems to be a problem of the small CRT used in the system.
The Polaroid system also requires a third party driver. Most of the problems with the driver seem to be resolved now, but there have been headaches with this also. The problem arises with objects that the driver can't convert. It also takes from 3-5 minutes for printing each slide.
Recently analog (splits the signal going to the monitor) slide makers that work at the PC screen frequency have become available. Previously they only worker with high end work stations .
The system we have has worked very well on our Pentium system. The resolution is excellent, anything displayed on the monitor shows up on the slide exactly the same, and it takes only a few seconds to print each slide. There is a slight jagged look to the text compared to the Post Script Polaroid system. (We have printed graphics, scanned images, gels, etc.)
David Leaffer Roche Bioscience david.leaffer-at-syntex.com
I was absolutely blindsided yesterday with the news that Baltec is gone. It seems that some old Balzers employees bought Baltec out and now call themselves Techno Trade {I hope that its not Tech No Trade}. All should be the same except for the great service that we got both in person and over the phone; Al and Pat are gone and may not be back....I put in a very good word for them to the new owners. Since I have six major pieces of equipment from Balzers and no service contracts the help I have recieved over the years, as well as the good humor, has saved me thousands of dollars.
Please call the new owners at (603) 622-5011, to find out how the new company impacts your lab and put in a good word for the old service reps.
I think it would make it possible for us to make a more relevant reaponse if you would tell us what kinds of materials you are interested in detecting in establishing whether or not your parts are "clean".
We have been using a Lasergraphics LFR-Plus film recorderfor 5 or more years. It has the following characteristics: A. Input file formats: PICT1, PICT2, TIFF, PhotoShop 2.0 and 2.5 B. Resolution and bit depth: 2048x1365 or 4096x2731 lines; 33 bit color. C. ISO-100 film output: 35 mm or 4x5 inch (Polaroid and sheet flim).
It has been very reliable, and it produces good images, graphics, and text. Lasergraphics also provides software updates via its BBS.
Russell E. Cook Electron Microscopy Center for Materials Research Argonne National Laboratory 9700 South Cass Avenue MSD-212 Argonne, IL 60439 (708)252-7194 FAX: (708)252-4798
University of British Columbia, Vancouver, BC, Canada
July 27 - August 4, 1996
Covering everything from basic microscopy to the technical considerations that define the highest levels of performance of the confocal microscope, this course will include:
* Quantitative confocal microscopy * Pixelation: The Nyquist Criterion * Lasers and laser tweezers * Objectives and aberrations * Scanning-systems * Wide field/deconvolution techniques * Detectors: operation and performance * Optimal pinhole size & photon efficiency * Dye design, characteristics and use * How to keep your cells alive * Two-photon excitation * Video-rate confocal imaging * Measuring ion concentrations * Display and measurement of 3D data * Digital hard copy and storage * Fluorescent & gold labeling of living cells * Backscattered light imaging.
Lecture demonstrations will be interspersed with hands-on laboratory exercises that will utilize all of the currently available commercial instruments for 3D microscopic imaging. Students will work in groups of three throughout the discussion and laboratory sessions, and will complete a live-cell 3D study on a specimen of their choice. At least seven, separate 3D microscopical workstations, attended by a technical staff of 15, will be available for student use. Overall, the teacher/student ratio will be more than 2:1.
International Academic Faculty:
* Jon Art University of Illinois * Milton Charlton University of Toronto * Rachel Errington Oxford University * John Heuser Washington University * Jim Pawley University of Wisconcon-Madison * Wallace Marshall U. of California, San Francisco * Ernst Stelzer EMBL, Heidelberg * Roger Tsien University of California, San Diego * Watt Webb Cornell University * Michael Weis University of British Columbia * Nick White Oxford University
International Commercial Faculty:
* Ernst Keller Carl Zeiss, NY * Paul Millard Molecular Probes, OR * Sigrid Myrdal Bristol-Myers Squibb, WA * K. Sam Wells Bio-Rad,,CA
TUITION
Tuition is $1,500 US. On receipt of the deposit, all students will receive preliminary assignments and a copy of the textbook, "Handbook of Biological Confocal Microscopy," (Plenum, 1995). The tuition fee includes one ticket for the opening reception and the banquet, the textbook and all handouts. Accommodations and meals are not included in the tuition fee.
APPLICATIONS
Applicants will complete a questionnaire to assess knowledge level and field of interest. Enrolment will be limited to 20 participants. Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with basic texts to read before the course begins. Application packages may be obtained from
Prof. James Pawley, Rm. 1235, 1500 Johnson Dr., Madison, WI, USA 53706. Phone: 1-608-263-3147, Fax 1-608-265-5315, Email: jbpawley-at-facstaff.wisc.edu
Application deadlines:
Applications forms requesting information on field of interest and level of experience must be received for screening by March 1, 1996. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 1996. In general, refunds of the deposit will not be possible.
DATES:
Applications must be received by Mar. 1/96 50% deposit due Apr. 15/96 Registration 3:00 - 5:00 pm Sat., July 27/96 Last class will end with lunch Sun., Aug. 4/96
***************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 1235, Engineering Research Building, FAX 608-265-5315 1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
Abstract: The analysis of small mineral specimens presents problems to the mineralogist. In the case of well-crystallized micromounts, the specimen is small and only a limited amount of material is available for analysis. Without the use of an electron microprobe, the analyst is obliged to use chemical microscopy techniques. These simple and often elegant tests are perfect for the analyst on a budget. Normally a combination of optical properties determination and a few chemical tests are all that are needed for confirmation of a mineral. Both these techniques require very small amounts of material. The techniques of "opening up," or soluabilization of minerals, will be discussed. Some of the older blowpipe methods are incorporated in the analytical scheme used in this study. A step-by-step analytical approach to specific cases will be outlined.
Directions to Forensic Analytical Specialties, Inc. (FASI): FASI is located near the east end of the Hayward-San Mateo Bridge, in a light industrial park at the corner of Depot and Cabot Roads. From Highway 92, take the Clawiter Road exit, travel north on Clawiter for approximately 3/4-mile to Depot Road. Turn left (west) on Depot and go all the way to the end of the road. Enter the first driveway past Cabot on your right and drive straight into the parking lot. Forensic Analytical is clearly marked on the building face. Look for signs directing you to the appropriate door. FASI has expanded an remodeled since we were last in their classroom, so the location will be a new one for us.
Contact: For further information, contact Stephen A. Shaffer, MicroDataware, 1-510-582-6624 (voice), 1-510-582-8295 (fax), or by email to sshaffer-at-microdataware.com. Mark your calendar NOW!!!!
************************************************************ * Stephen A. Shaffer * Publishers of The Particle Atlas * * MicroDataware * on CD-ROM. Developers of custom * * 2894 Tribune Avenue * image and database software for * * Hayward CA 94542-1637 * laboratories, specializing in * * 1-510-582-6624 voice * light and electron microscopy. * * 1-510-582-8295 fax * Email inquiries invited. * * sshaffer-at-microdataware.com * ************************************************************
Microscopists: In an attempt to minimize wear and tear on our MT2b, I'm setting up an old MT-1 for serial thick sectioning. The problem is that the specimen holders are missing. Does any one out there have some tucked away? E-mail me personally, rather than reply to the list. TIA Julian Smith III Biology Winthrop University Rock Hill, SC 29733 smithj-at-winthrop.edu 803-323-2111 (vox) 803-323-2246 (fax)
There are copies of the original code for image simulation of dislocations by the CSIRO group ( One Dis, & Two Dis) in the MMSLib, which is available via the MSA ftp site.
Log in to the host site:
ftp.msa.microscopy.com
username = anonymous password = your Email address
go to the directory called /1-Public/4-MMSLib/CTEM
look for the folders called ONEIDSAN and TWODISAN
You will have to get a local computer guru to help you compile the code for your local computer platform.
It is all in Fortran, which only dinosaurs like me use...
We have a client who is looking at deformed endothelial cells on cornea. He wants to look at them under SEM initially to locate their position and to take photos. He then wants to examine them in the TEM to reveal more information about their nature. Our problem is in marking the desired area in some way so as to locate the position for TEM preparation.
We have tried burning around the deformed cells with the electron beam but the results are not visible under LM so locating the cell(s) for TEM is extremely difficult. We have tried placing a TEM labelled grid over the area, looking for damaged cells in the grid holes, identifying their position, then cutting the cornea at the desired level - technical difficulties associated with this.
DOES ANYONE HAVE AN IDEA OF HOW WE MIGHT ACHIEVE OUR AIMS? IS THERE A TRIED AND TRUE METHOD FOR THIS APPLICATION?
Preparation : Basically the lens is glut then OsO4 fixed (helps with secondary electron emission), dehydrated in ethanol and CPD. Lenses are mounted onto stubs and gold coated. The coating must be thick enough to prevent charging and heat damage by the beam.
I just used the last of my supply of King Cole Tea, a product of Canada. Any members of theis list in Eastern Canada wh would be willing to send me a box or two of the 250 sachet, gauze bag variety? I'd be happy to pay expenses+.
Ron Residence in Attleboro MA lherault-at-acs.bu.edu
} I then take the dried parts - diff pump casing, stack parts - } over to my campus Scientific Apparatus shop where they use a glass bead blaster } to remove the burned on dark deposits that are virtually impossible to remove } with detergents or by scrubbing. It works beautifully, the bead stream quickly } "melts" the deposits off leaving a fresh clean metal surface that your new oil } charge will just love to boil up against! } } Try to find a shop with a bead blaster. You may have to go to your local auto } body shop if your facility has none available. Hope this helps. Gib } Dear Gib & all, If you can't find a shop with a bead blaster, buy an air eraser kit from Scientific Instrument Services (908) 788-5550. The kit was { $100 when I bought one. You'll need a hood and compressed air. A small regulator for the compressed air is also needed, unless that is built in to your air supply. Good luck. Yours, Bill Tivol
I have been using an MT-6000 for several years now and am very satisfied with it. I have only used a 7000 on demo, but thought it to be a very nice machine and well worth the price. Good luck in your search.
On Wed, 18 Oct 1995 kaurin-at-rmslab.rockefeller.edu wrote:
} Date: Wed, 18 Oct 1995 10:23:47 EDT } From: kaurin-at-rmslab.rockefeller.edu } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Best Microtome } } What is the best microtome to purchase? I have used and enjoyed } an Ultracut E. However, they are no longer being made. Leica now has a } motorized/digital Ultracut S which I currently use. Although it is a nice } machine, it is difficult to face quickly with a glass knife due to the greater } resistance of the wheel. (my samples will fracture with razorblade facing) } Another facility has asked for our input on a microtome purchase. They are } considering a RMC MT-7000. Is this a good model? Are there any used ultracut } Es for sale? I look forward to hearing your views and suggestions. } Shelley Landon Kaurin } } }
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % % % Daniel Possin Work: 206/ 543-7489 % % Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 % % University of Washington Home: 206/ 778-1714 % % Seattle WA 98195 USA Email: oemlab-at-u.washington.edu % % % % "The chinese expression 'cheung meng ba sui, gong hey fat choy' is % % equilvalent to Vulcan expression 'live long and prosper'. It's a % % small universe and getting smaller everyday". % % % %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
} Does anyone in the group know the telephone number of the company } called Kevex, (I may have spelled it wrong), this I believe is the company } that manufactures EDS equipment. If I could have either the telephone number,
I use 1-800-495-3839. They list 805-295-0019 and Valencia, CA on the back of their brochures.
Just a user. No endorsement implied. ---------------------------------------------------- Warren E. Straszheim 270 MD Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091 E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
coal characterization and processing electron microscopy, x-ray analysis, image analysis computer applications
I'd like to know whether anyone knows of a source for a dye called Chlorazol Paper Brown (also identified as "Michrome No. 94"). The stain is cited in Gurr's Encyclopedia of Microscopic Stains (1960), with the original reference given as: Verdcourt, B., 1947, Chlorazol Paper Brown B as a stain for plant tissue. Stain Tech 22:155-156. The dye is a dis-azo series acid dye. I have been unable to find it in any of our catalogs and would like to try it. If anyone can point me in the right direction or, perhaps, provide me with a small amount, I would apreciate it greatly.
Thanks in advance!
Dwight Beebe Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca Dept. de sciences biologiques Voice:514-872-4563 Universite de Montreal FAX:514-872-8496 4101, rue Sherbrooke est Montreal, PQ H1X 2B2 Canada
If it is appropriate, please post this on the Bulletin Board. Thanks, Tom Kelly
Microscopists: Please note the Symposium below. **************************************************** CALL FOR PAPERS
INNOVATIONS IN INSTRUMENTATION FOR MATERIALS RESEARCH Symposium AA at the 1996 Spring Meeting of the Materials Research Society April 8 to 12, 1996, San Francisco, CA
We are in the midst of an explosion in new technologies for microscopy, spectroscopy, data analysis, sample manipulation and more. The materials community is central to much of this activity and both drives and embraces these developments. The need for new instrumentation is especially poignant now with the increasing need to understand materials on the atomic scale and with greater precision in physical properties measurements. This symposium will concentrate on the innovations and the people who develop the innovations. Analytical characterization of materials including at least imaging, elemental composition, chemical properties, and mechanical testing on a microscopic scale will be considered.
To our knowledge, there has never been a symposium which specifically seeks to bring together the developers of the instrumentation for the purpose of reviewing the latest innovations in the technology. We expect both the developers and users of instrumentation to be interested in this symposium. Academic, industrial, and commercial groups developing either systems or components which advance the state of instruments used for materials research are invited to participate. We expect a cross-fertilization among several communities. Examples of topics of interest include:
1) System Components a. Radiation Sources b. Optics c. Motion Technology, Stagework d. Sensors and Detectors e. Attenuation of Environmental Detriments
2) Integrated Systems a. Microscopy and Spectroscopy b. Microscopic Mechanical Test Systems
3) Enabling Instrumentation and Manufacturing a. Microfabrication b. Computer Hardware and Software
Invited speakers include: Ruud Tromp (IBM Watson Research Laboratory), Pupa De Stasio (Swiss Institute of Technology), Noel MacDonald (Cornell Univeristy), Ronald Gronsky (University of California - Berkeley), Ondrej Krivanek (Gatan), Kamal Soni (University of Chicago), Jon McCarthy (NORAN Intruments), Heiner Jaksch (Zeiss), David Williams (Lehigh), Hans Hallen (North Carolina State University), Franco Cerrina (University of Wisconsin), Michael Miller (Oak Ridge National Laboratory), Jan Ringnalda (Philips), Warren Oliver (Nano Instruments Inc.), and more.
It is expected that some exceptional contributed papers will be upgraded to longer, invited presentations.
Symposium Organizers:
Thomas F. Kelly Dept. Materials Science & Eng. University of Wisconsin 1500 Engineering Drive Madison, WI 53706-1607 Phone: 608 263-1073 Fax: 608 263-1087 tfkelly-at-engr.wisc.edu
Paul E. Fischione E. A. Fischione Instruments, Inc. 9003 Corporate Circle Export, PA 15632 Phone: 412 325-5444 Fax: 412 325-5443 paul.fischione-at-internetmci.com
Lorretta Inglehart National Science Foundation Division of Materials Research 4201 Wilson Boulevard, 1065 S Arlington, VA 22230 Phone: 703 306-1817 Fax: 703 306-0515 lingleha-at-nsf.gov
David B. Williams Dept. Materials Science & Eng. Lehigh University Whitaker Lab #5, 5 E. Packer Ave. Bethlehem, PA 18015-3195 Phone: 610-758-4224 Fax: 610 758-4244 dbw1-at-lehigh.edu
**************************************************** Abstract Model - 1996 Spring Meeting Abstract Deadline: November 1, 1995 Submit Abstract to: ATTN.: ABSTRACT ENCLOSED Materials Research Society 9800 McKnight Road Pittsburgh, PA 15237-6006 Fax: (412) 367-4373 Submitted to Symposium AA Symposium Title: Innovations in Instrumentation for Materials Research TITLE OF PAPER - ALL CAPITAL LETTERS This set of instructions is given in the style and format to be used by authors in preparing abstracts. Follow this example in preparing your abstract. Reproduction will be done by photographing the abstract copy exactly as received and reducing it in size by 33%. You may use the special templates for this purpose obtainable from symposium organizers or
MRS Headquarters; our you may submit your abstract on white paper, in a space no larger than 5 in. (12.7 cm) wide by 5 in. (12.7 cm) long. At the top of the page above the text, you must include the name of the meeting and symposium title; at the bottom, or on a separate page, list all authors (full name, complete mailing address, phone, fax. e-mail).
Input Instructions
Type the title in capital letters, single space. Begin the text two lines below the last line of the title: single space, with double spacing between paragraphs.
Printing Guidelines
If you use a word processor or computer, print using a letter-quality printer (e.g., impact or laser printer - do not use a dot matrix printer) or use an electric typewriter with carbon ribbon.
Abstract Submission
Submit one camera-ready abstract to MRS Headquarters. Contributors must observe established deadlines to ensure being listed in the Program book.
Contact Author: MRS ID No. (if known) Last Name First Name Institution Department Street/P.O.Box City State/Zip Country Telephone FAX E-mail
Presenting Author: MRS ID No. (if known) Last Name First Name Institution Department Street/P.O.Box City State/Zip Country Telephone FAX E-mail
Co-Author: MRS ID No. (if known) Last Name First Name Institution Department Street/P.O.Box City State/Zip Country Telephone FAX E-mail
Co-Author: MRS ID No. (if known) Last Name First Name Institution Department Street/P.O.Box City State/Zip Country Telephone FAX E-mail
Please check if appropriate: Duplicate of fax submitted earlier Corrected/revised copy of abstract submitted earlier Change in title/author Change in text MRS Log No._______________________________ Poster Presentation preferred Note: If you send your abstract via fax: please wait to receive an acknowledgment letter with the assigned MRS Log No. Fill in the MRS Log No. on the line above, and send your camera-ready abstract to MRS.
MRS, please send: Mark with X. A 1996 Spring Meeting Graduate Student Award Application to ___________________(graduate student and an author of above abstract) A 1996 Spring Meeting Symposium Aide Application to ___________________(graduate student)
Thomas F. Kelly Internet: tfkelly-at-engr.wisc.edu University of Wisconsin Phone: 608 263-1073 1500 Johnson Drive Fax: 608 263-3704 or 3-1087 Madison, WI 53706-1687
I need information on exactly how did the "Gentleman Jim" slam freezer obtain its name. Some have thought it came from the champion boxer, James Corbett, who was later recognized as "Gentleman Jim". He bacame famous for his hard left hook. If anyone out there can give me information on this, I would appreciate it. Thank you in advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA.
Does anyone know of a good microprobe standard for Co other than Co metal? I have tried and failed to find alternatives, such as oxides, that can be obtained in sufficiently large single crystals (i.e. } 10um). What about Co-bearing silicate glasses? Silicides? Any help would be greatly appreciated.
Roy Christoffersen Texas Center for Superconductivity 3201 Cullen Houston, TX 77204-5932 roy-at-bayou.uh.edu (713) 743-8273 FAX: (713) 743-2787
Subscribe Microscopy mgarment-at-facstaff.wisc.edu Martin B. Garment Ophthalmology and Visual Sciences 1300 University Ave. Rm. 6687 Madison WI 53706 Voice (608) 262-9596 Fax (608) 262-0479 Email - mgarment-at-facstaff.wisc.edu
Could someone help us to find where we can buy this film (Kodak film 5302 kodak 35 mm CAT Number 166 7229 Eastman Fine grain)? Our EM (Philips) only works with 35 mm film and the best results we can obtain is with this film, but we cannot find it in Brazil. We want to import it using our credit card, by mail.
Thanks in advance
============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 Departamento de Ciencias Basicas/Histologia| r. 278 Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8191378 BRAZIL | ==============================================================================
In message Tue, 24 Oct 1995 12:09:00 +0000, kelloes-at-emlab.cb.uga.edu writes:
} I need information on exactly how did the "Gentleman Jim" slam } freezer obtain its name. Some have thought it came from the champion } boxer, James Corbett, who was later recognized as "Gentleman Jim". } He bacame famous for his hard left hook. If anyone out there can } give me information on this, I would appreciate it. Thank you in } advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA. ---------------- My understanding is that the name came from the fact the specimen is not just slammed against a cold metal block as in some devices (eg."Slammer") that can produce repeated mini bounces. "Gentleman Jim" has a shock-absorbing glycerine-filled device that absorbs the impact of slamming, prevents the mini bounces and yet holds the specimen firmly against the cold block. That is, it handles the specimen relatively "gently" but firmly!
************************************************************************* M.V. Parthasarathy Professor of Plant Biology & Director, Cornell Integrated Microscopy Center Section of Plant Biology 228 Plant Science Building Cornell University, Ithaca, NY 14850 Telephone: (607) 255-1734 Fax: (607) 255-5407 E-mail: mvp2-at-cornell.edu ***********************************************************************
We need to measure the beam current in our variable pressure SEM to improve our quantitative x-ray analysis using EDX. (Our SEM does not have a specimen current meter, so we can not fabricate one as recently described by S.D. Walck in Microscopy Today.) Does anyone make a simple, inexpensive unit with a meter for measuring the current accurately? We could build one if plans/specifications were made available.
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
} I need information on exactly how did the "Gentleman Jim" slam } freezer obtain its name. Some have thought it came from the champion } boxer, James Corbett, who was later recognized as "Gentleman Jim". } He bacame famous for his hard left hook. If anyone out there can } give me information on this, I would appreciate it. Thank you in } advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA.
I remember asking Alan Boyne, the designer of the "Gentleman Jim" why he named it that. As I remember, he told me that everyone was talking about 'slam freezing' which sounded like a very distructive way to freeze biological tissue, hence his use of "Gengleman Jim", implying a very gentle way to freeze tissue. It had nothing to do with the above referenced fighter.
Herb Hagler, Ph.D. Director of Computer-Assisted Instruction for Southwestern Medical School UT Southwestern Medical Center (214)648-3890 fax(214)648-3925
Cathy Kelloes asks: "I need information on exactly how did the "Gentleman Jim" slam freezer obtain its name." Well, it's like this... The first commercial device to utilize the "slam freezing" technique was the "Slammer" from BioRad. The problem with this device turned out to be that the specimen would "bounce" against the freezing surface after the first impact, distorting the morphology. Alan Boyne, of the University of Maryland, Baltimore, figured out how to avoid "bounce" by softening the impact of the specimen against the freezing surface (a copper anvil) using a hydraulic mechanism. He introduced his new instrument at a Cell Biology meeting in the mid-80's as "Gentleman Jim", the implication being that, unlike the rough treatment of the specimen by the Slammer, the new instrument would be gentle on the specimen. Immediately after the meeting,Alan Boyne signed an exclusive distribution arrangement with Ted Pella, Inc., who kept the name. The rest, as they say, is history. Alan Boyne now lives in Friday Harbor, Washington, in a cabin or yurt or some such thing which he built himself. By now, he may have a phone. Steven Slap P.S. The "Gentleman Jim" is still available through Energy Beam Sciences.
On Tue, 24 Oct 1995 kelloes-at-emlab.cb.uga.edu wrote:
} I need information on exactly how did the "Gentleman Jim" slam } freezer obtain its name. Some have thought it came from the champion } boxer, James Corbett, who was later recognized as "Gentleman Jim". } He bacame famous for his hard left hook. If anyone out there can } give me information on this, I would appreciate it. Thank you in } advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA. } I am not sure how much I can add to the discussion, but my recollection from early conversations when the Gentleman Jim was first introduced was that the name was chosen as a counterpoint to the term Slam Freezer, emphasizing that the tissue was not damaged in the slamming process.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
} Does anyone know of a good microprobe standard for Co other than Co metal? } I have tried and failed to find alternatives, such as oxides, that can be } obtained in sufficiently large single crystals (i.e. } 10um). What about } Co-bearing silicate glasses? Silicides? Any help would be greatly } appreciated. } } } Roy Christoffersen } Texas Center for Superconductivity } 3201 Cullen } Houston, TX 77204-5932 } roy-at-bayou.uh.edu } (713) 743-8273 } FAX: (713) 743-2787
Personally, I use a cobalt olivine (Co2SiO4) for most silicate analysis. It was synthesized a number of years ago, and I'm afraid I don't know where more material is located.
You might give Bart Cannon at Cannon Microprobe a call:
Cannon Microprobe 1041 NE 100th St. Seattle WA 98125 206-522-9233
I see that he has several Co standards, of which a CoAl2O4 looks promising. I have not yet purchased any material from Cannon, so I cannot vouch for this or any other material.
Geller Microanalytical has two cobalt standards (Co3O4 and CoSi2) that would be good candidates (their new number is 508-887-7000).
Finally, the so-called Corning glass standards that the Microbeam Analysis Society used to distribute (and I am now by default the person in charge of these materials) have nominally 0.8 % of a suite of elements in a CaMgSiB glass. One of these glasses contains Co at about this concentration. These glasses are useful as secondary standards, not for primary calibration. They also suffer from a lack of complete agreement on the compositions from various techniques.
I have no connection with either of these commercial sources.
Good luck,
Paul Carpenter
+---------------------------------------------------------------------------+ | Paul K. Carpenter paulc-at-arms.gps.caltech.edu | | Division Analytical Facility Department of Geology 170-25 | | California Institute of Technology Pasadena CA 91125 | | 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) | +---------------------------------------------------------------------------+
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
For some years, I have had the opportunity to visit EM laboratories virtually all over the world, and believe it or not, the subject of which is the "best microtome" seems to come up even more often than which is the "best electron microscope". Perhaps I am asked questions like that because we have absolutely no vested interest in our answer and people know that.
And I have come away from such conversations realizing that the single strongest factor in customer satisfaction in terms of microtomes if not most things is based on the quality of local service. I stress the word "local" because the quality of the service in one city or country has no relationship (from what I can see) with the level of service in some other location in the world (or even how close or far one might be located relative to the factory making the microtome). Some times the high quality service does not come from the manufacturer at all, but from a "third party" service organization, and in some instances, they will service any brand of ultramicrotome.
To evaluate the quality of the local service that is available, instead of going to the biggest name person and getting their opinion, go to the laboratory that runs the highest volume of samples. Find out how much down time they experience. If the down time is relatively low, then they are getting good service. You don't even have to ask about the quality of the sections, the two major manufacturers both make excellent equipment. But "happiness" with an ultramicrotome is synonomous with how happy one is with their local service provider.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
} Does anyone know of a good microprobe standard for Co other than Co metal?
We use a Co-sulfide with success. Unfortunately I do not know the source or do we have any extra!
Carl
Carl Henderson Electron Microbeam Analysis Laboratory University of Michigan 2005 C.C. Little Bldg. Ann Arbor, MI 48109-1063 USA -------------------------------- (313) 936-1550 (voice) (313) 763-4690 (FAX) chender-at-umich.edu (e-mail) --------------------------------
Does anyone know the address or phone # of a North American agent for Narishige Instruments? We have a motor driven micromanipulator which we bought several years ago that now needs service. Unfortunately the contact number we had in Japan is out of service.
Many thanks
Richard
------------------------------------------------------------ Richard J. Mount Phone: 416-813-6551 Auditory Science Laboratory Department of Otolaryngology FAX: 416-813-5036 The Hospital for Sick Children 555 University Ave. Toronto, ON, Canada M5G 1X8
As Alan Boyne's graduate student and one who was intimately involved with the Gentleman Jim's development, I would like to confirm that Alan named the Gentleman Jim as a subtle dig at the "slammer" which was the principal alternative at the time. He was, of course, well familar with the famous boxer of the same name. Alan is living on Friday Harbor and his phone number is 206-378-7252. If you want any more details on the Gentleman Jim feel free to contact Alan or myself. By the way, I don't think Alan has a distributor anymore for the Gentleman Jim but I think he would be willing to provide one for anyone who is in the market.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
I can recall, that as a callow youth we used Zamboni' fixative. What I do not recall is its composition.
If there any one out there who can supplement my memory, I would appreciate the recipe. -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 904-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax 904-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
{Text_1} On Oct. 22, Felicity Lawrence Inquired about marking corneal SEM specimens for location during TEM. Many years ago we had the same problem using cochlear specimens. Our best results were achieved by bolting a micromanipulator (de Fonbrune type, pneumatic) into one of the ports of a JEOL JSM35 SEM. Although making 3D manipulations while observing a 2D image was difficult we were able to scratch delimiting marks around areas of interest. These marks could be seen in the embedded specimen and, if cutting at right angles to the surface, in thick sections. The procedure was only marginally successful on gold coated specimens as the scratched area would charge, however, using the O-T-O-T-O technique we were very successful. This technique has the added advantage that sections cut for TEM do not need staining as there is sufficient osmium to provide contrast.
Hope this info. is of some help, best of luck,
Richard
------------------------------------------------------------ Richard J. Mount Phone: 416-813-6551 Auditory Science Laboratory Department of Otolaryngology FAX: 416-813-5036 The Hospital for Sick Children 555 University Ave. Toronto, ON, Canada M5G 1X8
} Does anyone know of a good microprobe standard for Co other than Co metal?
Actually, Co metal is pretty good for a cobalt standard. The mass absorption coefficients for Co Ka in an oxygen and sulfur matrix are:
Co Ka by Oxygen, mac 17.5 Co Ka by Sulfur, mac 143.7
Co Ka is a hard x-ray line such that it doesn't really matter what the matrix is for light elements like this. However, if you are looking at Co in an Fe matrix, the mac is much higher, since Fe Ka is fluoresced by Co Ka (and hence Co Ka is absorbed):
Co Ka by Fe, mac 401.9
So if you need to analyze Co in stainless steel or Fe-bearing spinel, then it may be a good idea to find a well-characterized Fe-bearing Co standard. But in general (for silicate systems with low Fe), the absorption correction is going to be fairly small, and if Co is a trace element, the dominant problem is just getting some x-ray counts to begin with.
Now light element measurement, that is where standards must be carefully chosen.
Paul
+---------------------------------------------------------------------------+ | Paul K. Carpenter paulc-at-arms.gps.caltech.edu | | Division Analytical Facility Department of Geology 170-25 | | California Institute of Technology Pasadena CA 91125 | | 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) | +---------------------------------------------------------------------------+
The main reference is: Stefanini et al., 1967, Nature 216:173.
Kent A. Kent Christensen, Univ. of Michigan, {akc-at-umich.edu}
----------------------------------
On Wed, 25 Oct 1995, Greg Erdos wrote:
} I can recall, that as a callow youth we used Zamboni' fixative. What I do } not recall is its composition. } } If there any one out there who can supplement my memory, I would appreciate } the recipe. } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- } Greg Erdos Phone: 904-392-1295 } Scientific Director, } ICBR Electron Microscopy Core Lab } 218 Carr Hall Fax 904-846-0251 } University of Florida E-mail: gwe-at-biotech.ufl.edu } Gainesville, FL 32611 } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- } }
Does anyone have any ideas on how to combine data from Electron microscopy reconstruction and X-ray crystallography in image analysis when the complex structure obtained from EM and some components' structure came from CRYSTALLOGRAPHY?
Many thanks!
Jinghua
--
$&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&$ $ Institute of Molecular Biophysics 206 Rogers Hall,Ubox 65455 $ $ Florida State University Florida State University $ $ Tallahassee, FL 32306-3015 Tallahassee, FL 32323 $ $ (904) 644-1115 (904) 853-1498 $ $ jinghua-at-sb.fsu.edu http://www.sb.fsu.edu/~jinghua $ $&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&$
Due to space restrictions (yes, even in a practically empty country like New Zealand!) we have had to dismantle the still with which I used to produce our own super-dry ethanol.
From now on we will be using commercial absolute ethanol for EM specimen dehydrations, and I am seeking advice on what to put in the opened containers of ethanol to absorb any traces of water. I never cared much for molecular sieve pellets because of the dust that comes off them.
Any suggestions on what to use, the quantity required and its "lifespan" in use, and how to package the desiccant within the bottles, will be gratefully received and a summary posted to the list.
Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department School of Medicine University of Auckland Private Bag 92019 Auckland New Zealand
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
On Oct. 24, F.J. Hernandez asked about where he could purchase Kodak 5302 35 mm roll film.
Actually, this product is available from most of the major suppliers of EM consumables, such as SPI Supplies.
Naturally I am a bit biased in terms of which one you should consider first, but just about all of the major EM consumables suppliers handle this particular Kodak product. With the heightened security precautions in effect worldwide, make sure you ask your vendor to place special "do not x-ray" stickers on the boxes, other wise you will have rather foggy micrographs, which I am sure you don't want to have. You also have to realize that even with the "do not x-ray" stickers, there is still the risk that the box will be x-rayed and the risk of that happening is on the customer, not the supplier.
At the present time, we advise NOT to send your VISA card number and expiration date over the internet. That is what the experts say. You should send it by FAX which is more secure. When you do that the first time, with SPI, you can also give authorization to use the same card in the future by special authorization code, that is in the future, for additional orders, you can just refer to your VISA card number on file via the internet but without actually "giving" your number again.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za} Gardnerjs-at-yvax.byu.edu (flavio ortolano gardnerjs)
Dear John I have a Durst M670 enlarger and I use a combination of the VEGATUB 39 lens board with a 50 mm lens to make transparencies of 6x7 cm negatives. (Note: The condenser must match the negative size.) The enlarger is racked down to about 10 cm from the easel, and by manipulating the racking and focussing controls you will be able to vary the size of the final (focussed) image.
What is actually taking place by doing this is that the object (negative) is placed outside twice the focal length of the lens, resulting in demagnification. If you require something intermediate between a large format and a 35mm size, I suggest using an 80mm lens.
I hope this helps you.
James Wesley-Smith Electron Microscope Unit George Campbell Building University of Natal Durban, South Africa
A student here wants to fix and store bee's for a prolonged period of time. Any suggestions as to stop at primary fixation or after dehydration. Which buffer is preferrable i.e. Na-cacodylate or Phosphate buffer.
Thanks
Stephan H Coetee Electron Microscope Unit Private Bag 3 Wits 2050 Stephan-at-Gecko.biol.WITS.ac.za
Could anyone advise me which (MAC) NIH IMAGE version is compatible with the IBM -MAC emulator 'executor'. I have tried v1.57 and 1.47 and get an error message relating to a lack of qiuckdraw with v1.57 and v1.47 just crashes
Thank you
Brendon Brendon J. Griffin Centre for Microscopy and Microanalysis The University of Western Australia Nedlands, WA, AUSTRALIA 6907 ph 61-9-380-2739 fax 61-9-380-1087
Message-ID: {MAPI.Id.0016.00683536202020203832384530303034-at-MAPI.to.RFC822} Priority: Normal To: LISTSERVER {microscopy-at-Sparc5.Microscopy.Com} MIME-Version: 1.0
For those of you interested in experimenting, I have found a source of foils 0.025mm thick of the following cobalt compounds: C069/B12/Si12/Fe4/Mo2/Nil Co66/Si16/B12/Fe4/Mo2 Co66/Si15/B14/Fe4/Nil Steven Slap, Vice-President Energy Beam Sciences, Inc.
Un subscribe Michael George, mgeorge-at-dubois.fisk.edu
Michael A. George, Ph.D. ******************************************* Research Assistant Professor Department of Physics Fisk University Nashville, TN 37208
Since we're on the subject, I'd be interested to know if anyone has actually had any micromanipulators rebuilt or repaired by Narishige-I know someone here in Souther California that works on Narishige electronics, but never have found anyone to do manipulators. Incidentally, Narishige was useless in finding parts or repairs for the instrument I needed fixed (vertical micropipette puller)-I just got lucky by making a lot of phone calls. Grace Kennedy
} From: USERHHXS-at-um.cc.umich.edu } Date: Wed, 25 Oct 95 12:03:20 EDT } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Spurious Mo peaks
} We're getting large Mo peaks (and nothing else) from our Be window } EDS detector, even without a sample holder in place. Another } point of note is that when a bulk copper sample is inserted into } the beam path, the same Mo peaks appear along with an additional } pair of peaks a few eV lower in energy (these do not correspond } to any realistic elemental peaks. Any thoughts as to the origin } of these peaks. As far as I know, the only Mo on the column } would be the aperture strips.
To understand your problem, it would be of help to know if the microscope is a TEM or a SEM, telling something about the geometry of the detector and samble holder in the specimen stage.
At which energies do the "false" peaks occur? One of them is probably the Mo escape peak.
When you insert the copper specimen, do you see the copper peaks along with the molybdenium peaks? If not, your collimator is probably misaligned (out of position), and/or the specimen holder is not properly aligned with the detector. The detector "sees" something else than the sample. This something is most certainly made of molybdenium. Detectors are usually installed so the sample is in the center of the detector=B4s "visual field" when the sample altitude is adjusted to the eucentric point of the goniometer.
Is the detector close enough to the sample? If not, the acceptance angle of the collimator allows the detector to "see" to much of the surroundings of the sample, e.g. the mechanisms of the sample holder.
Good luck!
Rolf Odselius ________________________________________________________________________ Rolf Odselius, PhD, Ass Prof Electron Microscopy Unit, University Hospital S-221 85 Lund, Sweden Phone: +46 46 171075 Fax: +46 46 172975 Mobile phone: +46 10 6705655 Pager (Minicall): +46 740 288992 E-mail: Rolf.Odselius-at-emu.lu.se URL: http://www.medfak.lu.se/medinst/emu/ Educational Secretary, SCANDEM URL: http://www.ldc.lu.se/~scandem/
--Boundary (ID PACtFaIgLPtJuQ00ZMEzQg) Content-type: TEXT/PLAIN
Good Morning All,
I'm wondering if anyone can suggest a simple method for preparing cross sections of thin ( {1mm) metal sheet by electropolishing.
I routinely do this using the tried and true(?) wafer stack, dimple, ion mill method, but would like to find a quicker, Epol based method if possible.
I'm ruling out tripod polishing - ion milling for now (though I haven't tried it yet) on the basis of the time (I think is) required.
My ideas run along the "jam" an ultrasonically cut stack into a 3mm tube and hope for electrical contact route, but I'd appreciate some other ideas.
I recall seeing a poster once where a dimpling apparatus had been modified to electopolish or at least chemically polish while dimpling. Did that line of work ever continue?
I have have some colleagues who'll be interested, so please respond to the server if you have any ideas on the matter.
Thanks in advance,
############################################################### # # # Don Steele STEELE-at-KRDC.INT.ALCAN.CA # # ALCAN INTERNATIONAL # # Kingston Research and Development Center # # P. O. Box 8400 # # Kingston, Ontario Canada K7L 5L9 # # # ###############################################################
Stephen Edgar {s.edgar-at-auckland.ac.nz} wrote: } } } From now on we will be using commercial absolute ethanol for EM } specimen dehydrations, and I am seeking advice on what to put in the } opened containers of ethanol to absorb any traces of water. I never } cared much for molecular sieve pellets because of the dust that comes } off them. } Any suggestions on what to use, the quantity required and its } "lifespan" in use, and how to package the desiccant within the } bottles, will be gratefully received and a summary posted to the list.
I have used molecular sieve encased in dialysis tubing, which seems to have prevented any obvious dust problems. But unless the sieves themselves are scrupulously dry, they can acutally ADD water to your solvent. For freeze substitution work, I have had nice results with adding 1% acidified dimethoxypropane to substitution solvents (ethanol or acetone). This compound reacts chemically with water to produce acetone and methanol. Of course, I don't know if the benefits in freeze substitution come from simply having more strictly anhydrous reagents, or if the dmp actually speeds up substitution of the tissue. I should also mention, that most of my work is at the light micrscope level, so I also can't comment about ultrastructure. But I like the concept of removing water chemically, with dmp, as opposed to physically with a sieve. Hope this helps, Tobias Baskin
A friend has a Hitachi S570 SEM that uses Lion-S (Japanese company) diffusion pump oil and needs to replace some of the oil in his 500W dp's. Anyone know of a vendor in the US? Thank you very much.
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
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Would like to know if there is any way to selectively etch platinum on a GaAs substrate. Has anyone tried this or are they currently doing this???
I understand that aqua regia would remove platinum but have been advised that it also attacks GaAs. Are there any solutions that would slow down the rate of attack on GaAS and retain effectiveness on platinum???
Rich Sartore Army Research Laboratory AMSRL-PS-DC Fort Monmouth, NJ 07703 rsartore-at-arl.mil 908-427-2261
Some of us,although we would love to get a little free advertising on the net,are absolutely scrupulous about not mentioning our company names,etc on this listserver.We have been told that this is the rule and we are happy to abide by this because we enjoy using this medium.
I think this latest communication by Doctor Garber not only advertises his website catalog but actually gives ordering information for his company.This may be denied under the guise of aiding subscribers but this and other instances have in fact been advertising.
Just tell us if the rules have been changed or how we think the rule should be interpreted so that the playing field can be level for all
Group- Regarding inquiries in the recent past concerning laser printers: After considerable survey, I recently purchased a Lexmark Optra R laser printer - rated at 1200 dpi. The current price of the unit at "Best Buy" (a discount house) is $1,299.50. The unit, with 2K RAM, prints at 1200 dpi up to a certain, undefined "density" per page - and, when exceeded, reverts back to 600 dpi. If one always wants 1200 dpi, they recommend that you purchase an additional 8K RAM. I also understand that one of the computer magazines rated the unit as their "Best Buy." I am truly delighted with the printer. The print quality, as one would expect, is nifty. However, the ease of use and flexibily are equally outstanding. I recommend the unit very highly. In passing, should you be interested in quality, printed reproduction of images (as I am in our newsletter "Microscopy Today"), you may care to consider the front cover image on our last (October) issue. It is the first time that we used a (writeable) CD for input. Rather nice, yes? Regards to all, Don Grimes, Microscopy Today
} A friend has a Hitachi S570 SEM that uses Lion-S (Japanese company) } diffusion pump oil and needs to replace some of the oil in his 500W dp's. } Anyone know of a vendor in the US? Thank you very much. }
I substituted Octoil-S for Lyon-S a few years ago in our S570. Since then, however, I switched to a larger heater (700W) and changed over to Santovac 5 in an attempt to minimize oil contamination in the chamber. (BTW, that did not solve the problem...)
Carl
Carl Henderson Electron Microbeam Analysis Laboratory University of Michigan 2005 C.C. Little Bldg. Ann Arbor, MI 48109-1063 USA -------------------------------- (313) 936-1550 (voice) (313) 763-4690 (FAX) chender-at-umich.edu (e-mail) --------------------------------
} } From now on we will be using commercial absolute ethanol for EM } specimen dehydrations, and I am seeking advice on what to put in the } opened containers of ethanol to absorb any traces of water. I never } cared much for molecular sieve pellets because of the dust that comes } off them.
} Any suggestions on what to use, the quantity required and its } "lifespan" in use, and how to package the desiccant within the } bottles, will be gratefully received and a summary posted to the list.
} Regards
} Stephen Edgar
We use commercial absolute ethanol and acetone. We keep both dry with dehydrated copper sulphate, which is made by baking cuso4.5h20 in a crucible over a bunsen flame until the salt becomes a white powder. When its cool, we put it in a "sausage skin" of dialysis tubing (say 10 mm diam.), opened out and with a knot tied at one end. We tie off the other end and put the cus04 sausage in the solvent. Unless you actually put water into the bottle, it seems never to need changing. It is self indicating in that the cuso4 goes blue again in the presence of water.
Greetings, (Sorry if you get this twice--I never got the first one...)
Stephen Edgar {s.edgar-at-auckland.ac.nz} wrote:
} } From now on we will be using commercial absolute ethanol for EM } specimen dehydrations, and I am seeking advice on what to put in the } opened containers of ethanol to absorb any traces of water. I never } cared much for molecular sieve pellets because of the dust that comes } off them. } Any suggestions on what to use, the quantity required and its } "lifespan" in use, and how to package the desiccant within the } bottles, will be gratefully received and a summary posted to the list.
I have used molecular sieve encased in dialysis tubing, which seems to have prevented any obvious dust problems. But unless the sieves themselves are scrupulously dry, they can acutally ADD water to your solvent. For freeze substitution work, I have had nice results with adding 1% acidified dimethoxypropane to substitution solvents (ethanol or acetone). This compound reacts chemically with water to produce acetone and methanol. Of course, I don't know if the benefits in freeze substitution come from simply having more strictly anhydrous reagents, or if the dmp actually speeds up substitution of the tissue. I should also mention, that most of my work is at the light micrscope level, so I also can't comment about ultrastructure. But I like the concept of removing water chemically, with dmp, as opposed to physically with a sieve. Hope this helps, Tobias Baskin
Scott Walck asked about the de Fonbrune micromanipulators. They are made in the United States by Technical Products International. You should contact Ralph Willen at TPI at 314-522-8671 (phone) or 314-522-6360 (fax) for more information. Steven Slap, Vice-President
the following company in New York has a very good alternative to print images on Laserjet 3/4/4+ printers - it is a German interface board with 150, 300 and even 600 dpi printing capability, with a palette of 256 from 1024 gray levels. it works really fine for printing hires SEM images and prints a 2 MByte image in seconds!
the experience shows that it is a real alternative to photographic images when you have to find another way to print good pictures from a SEM/STEM.
contact : Electro Image, Inc. 9, Round Hill Road LAKE SUCCESS, NEW YORK 11020 tel 516 773 4305 fax 516 773 2955
Microscopists: A student and I are trying to do SEM of wheat chromosomes (with the ultimate goal of gold-labelled in-situ's). We have the nice chapter by Harrison and Jack in Hayat's "Correlative Microscopy", and the Scheid and Tarut citation therein on Vicia faba. Anyone out there have anything more recent to point us to? TIA Julian Smith III Biology
Don, how are you? The most importante piece of information is missing from yours and other's answers!. How long it takes to print a 8x10 page at 1200dip? I received a flyer from Nat. Graphic Supply (800-223-7130) advertising a 1200x1200 printer name XANTE (no price given), but do not know anything about it. Regards, Cesar. _______________________________________________________________________________
} Due to space restrictions (yes, even in a practically empty country } like New Zealand!) we have had to dismantle the still with which I } used to produce our own super-dry ethanol. } } } From now on we will be using commercial absolute ethanol for EM } specimen dehydrations, and I am seeking advice on what to put in the } opened containers of ethanol to absorb any traces of water. I never } cared much for molecular sieve pellets because of the dust that comes } off them. } } Any suggestions on what to use, the quantity required and its } "lifespan" in use, and how to package the desiccant within the } bottles, will be gratefully received and a summary posted to the list. } } } Regards } } Stephen Edgar } } Electron Microscope Unit, Pathology Department } School of Medicine } University of Auckland } Private Bag 92019 } Auckland } New Zealand } } email address: s.edgar-at-auckland.ac.nz } Phone : +64-9-3737599 extn 6473 (GMT + 12h) } Fax : +64-9-3737459 } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } .} } }
We place the molecular sieve inside a bag of dialysis tubing in order to avoid the dust problem and add a bit of indicator silica gel to determine when the useful life of the desiccant is over. This doesn't work for acetone since the indicator dye is soluble . -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 904-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax 904-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Dear Sir, Please post this notice to your calendar. Thank you.
Ron Miller e-mail: image-at-imsworld.com
Neuroimmune Summer Workshop for Students and Faculty
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Lecture Topics (in part): Opiate Neurobiology and Immunology Stress Significance of microglia in psychiatry Psychoneuroimmunology
Laboratory Experience (in part; Hands-On) HPLC and RIA of neuropeptides Nitric Oxide-amperometric probe Image Analysis of Immunocytes In situ hybridization Cytokine determinations Confocal Laser Microscopy
Sponsored by the University of Modena, Image Analytics Corp., Nikon, Compix, World Precision Instruments and BAS
Scholarships are available - enrollment is limited
For application contact: Dr. Enzo Ottaviani, Dept Animal Biology Univ Modena, via Barengario 14, 41100 Modena Italy
In response to a number of questions received on the Lexmark Optra R: 1) Printing speed is 16 ppm at 600 dpi and 8 ppm at 1200 dpi and uses a 32-bit RISC processor. 2) To print a full 8.5" x 11" page of text, takes about 20 seconds from "go". 3) Toner comes in two packages - one for approx. 7000 pages is $179.95 and the other, for approx. 14000 pages is $265.95. These prices are out of the Misco catelog and one may be able to do better. 4) I previously mentioned the option of providing additional Printer Memory (I called it "RAM"). One can also purchase a Flash Memory Option to store information like downloaded fonts and macros. 5) The printer has two connections on the system board that can be used for either a Disk Option or a Network Option (or two Network Options, or one of each). Disk Option: A 40MB hard disk and a thumbscrew - for example, for downloaded fonts and macros. Network Option: To connect directly to a LAN - Token-Ring, Ethernet (10BASE2 or 10 BASE-T) or LocalTalk (whatever that is?). This option consists of a network card, a thumbscrew, a diskette containing the Network Printer Utility and documentation. You will need to provide the appropriate network cable. 6) I do not know the prices of any of the options but you can call (800)358-5835 for the name of a dealer near you. 7) In case of a problem, they will express an exchange printer OR repair and return yours. Apparently there is an on-site warranty repair service which I do not have. IBM, however, can provide local service. Lastly - in my previous note I may not have advised how REALLY easy this printer is to operate. I have had mine for a month now - and have not refered to the manual even once! Even in start-up, the printer "looked at" my computer software and adjusted itself to my previous printer driver. Prior to purchasing this unit, I looked at many high end laser printers and could not find any, at any price, that would even start to compare. I welcome any other inquiries, Don Grimes, Microscopy Today
At the request of Dr Garber,I am only too happy to identify myself to the list. My name is Norm Woodside and I am a manufacturer's representative for Ted Pella,Inc in the middle Atlantic area. If anyone was offended by this omission in my earlier message,for this I apologize. Otherwise, I stand by my comments. I will be happy to review any comments from the list members, but preferrably in private so as not to take up any netspace, unless of course you feel it of value to the entire list Thanks for your attention
} Date sent: Thu, 26 Oct 1995 15:47:45 -0600 } From: bozzola-at-siu.edu (John. J. Bozzola) } Subject: SEM: Lion S vacuum pump oil } To: microscopy-at-aaem.amc.anl.gov
} A friend has a Hitachi S570 SEM that uses Lion-S (Japanese company) } diffusion pump oil and needs to replace some of the oil in his 500W dp's. } Anyone know of a vendor in the US? Thank you very much. } } ############################################################################# } John J. Bozzola, Director } Center for Electron Microscopy } Southern Illinois University } Carbondale, IL 62901-4402 } U.S.A. } Phone: 618-453-3730 } Fax: 618-453-2665 } Email: bozzola-at-siu.edu } Web: http://www.siu.edu/departments/shops/cem.html } ############################################################################# } In Australasia Lion oil available through JEOL. It's the oil specified for several JEOL diff pumps, it's cheap and, in my old JEOL probe gives a better vacuum than does Fomblin.
Ritchie Sims phone: 61 9 3737599 ext 7713 Department of Geology fax: 61 9 3737435 University of Auckland Private Bag 92019 Auckland New Zealand
I'm curious about a fact that I have noticed when I'm studying EM sections. Some colleagues have also observed this: the image observed in the EM fluorescent ecran appears to be poor contrasted when I begin for the first time, but after some minutes (15 or 20) the contrast of the material improves! When the grid is observed for the second time, another day, the contrast is even better! Why? The EM sections are like pizza, that is, they are better the day after? I'm just curious. Francisco Javier Hernandez Blazquez
I'm curious about a fact that I have noticed when I'm studying EM sections. Some colleagues have also observed this: the image observed in the EM fluorescent ecran appears to be poor contrasted when I begin for the first time, but after some minutes (15 or 20) the contrast of the material improves! When the grid is observed for the second time, another day, the contrast is even better! Why? The EM sections are like pizza, that is, they are better the day after? I'm just curious. Francisco Javier Hernandez Blazquez
QUESTION: } I'm curious about a fact that I have noticed when } I'm studying EM sections. Some colleagues have also } observed this: the image observed in the EM fluorescent } ecran appears to be poor contrasted when I begin for the first time, but } after some minutes (15 or 20) the contrast of the material } improves! When the grid is observed for the second time, another } day, the contrast is even better! } Why? } The EM sections are like pizza, that is, they are better the } day after? } I'm just curious. } Francisco Javier Hernandez Blazquez
RESPONSE: Due to the heating of the section by the beam as well as the vacuum conditions inside the microscope column, the plastic embedment is undergoing sublimation - similar to what happens during freeze etching. With removal of the plastic, the remaining osmicated portions of the section will increase in contrast. In addition, the plastic is stabilized in the beam so that drifting is minimized. This phenomenon is so beneficial that some of the newer microscopes have a special feature that automatically traverses the beam over the section to stabilize the section and etch away plastic. A much more perplexing phenomenon, however, is why pizza does taste better the next day. Perhaps some in-depth research is in order here. PEACE.
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
It seems to me that a manual of microscopy techniques would be extremely useful, better in several ways than a book. I've set out my thoughts on this in the following URLs (they contain identical information but access speed will be better from your closest site).
Europe - {URL:http://www.lars.bbsrc.ac.uk/micro/in-focus/9511.html} America - {URL:http://metro.turnpike.net/jefferie/in-focus/9511.html}
If the idea interests you, please take a look and let me have some comments. If there's enough response perhaps we could get a group of people discussing it more formally by e-mail to hammer out some consensus views.
} } IBM East Fishkill, NY has an immediate opening for the services of a } part time TEM professional. 10 to 30 hours/week for several months } on a renewable basis. Experience with TEM of semiconductors is } desirable. Interested individuals in the SE New York area may } contact Ron Anderson (ron-anderson-at-vnet.ibm.com) or } Dave Kruger (dkruger-at-vnet.ibm.com) for more information. } }
I have been asked to repost the section of the ground rules about advertising on the Microscopy Listserver. Here they are as extracted from the latest version of the FAQ file. Basically the rule is no advertising, but Email addresses etc... after your Sign-Off are acceptable. When I see anyone crossing the line so-to-speak you can all be assured that the individual will hear from me.
Your Friendly Neighborhood SysOp Nestor
-------------------------------
Here is the exerpt from the FAQ file.
--------------------------------
} Can I post an Advertisement? } ---------------------------- } } No, that does not fit within the bounds of this discussion forum. } } This listserver is not intended to be a Sales mechanism } for commerical organizations, but rather it is an open discussion area } about microscopy and microanalysis problems and solutions. If you are } an } organization and have equipment you wish to donate, } or sell, for nominal cost (i.e. no profit) then this is generally } an acceptable posting. If you are not sure then send a } copy of the announcement in question to Zaluzec-at-MSA.Microscopy.Com } and I will give you my opinion. An example of this type } would be an old decommissioned instrument which someone } is trying to give away for removal/shipping costs, that would } fit within the bounds of the purposes of this list. } } If you are a manufacturer, you are always welcome to observe/join } in any discussion at any times. We do ask that everyone, please } refrain } from overt sales pitches and/or commericalism. If a product } which you produce/sell can solve a problem or answer a question } raised by anyone on this list, then by all means feel free to } say so. Try to be brief about the product, state the simple facts } in a few (short) sentences and then offer to continue the discussion } with } any interested parties off-line. Alternatively you can give } sufficient information } so that individuals can download/access the relevant information. } Usually } it will be sufficient to just add your phone number } and/or Email address to the end of your message, } and you'll be contacted by anyone that is interested. } } Remember, please keep your comments about any product } you "sell" to a minimum. } } It is not out of line to provide your company name, Email } address or WWW site as part of your signoff/signature line, } at the end of ANY message you post to this system. } } This Listserver operates on the honor system with respect to } to posting of advertising, so please respect these simple ground rules. } } If you are interested in using the Internet for Commerical Advertising } of Microscopy/Microanalysis Related Products/Services, you may wish } to } contact MSA at it's WWW site (http://www.msa.microscopy.com) or the } MSA Business Office (MSABusinessOffice-at-MSA.Microscopy.Com). } These alternative Internet services, are provided independently of } the } Listserver Operation, which MSA provides as a FREE service to the } WorldWide } Microscopy and Microanalysis Community. Any funds derived from } the above are used to defray the costs of running MSA's Internet site. } } -----------------------------------------------------------------------
John J. Bozzola wrote in response to Francisco Javier Hernandez Blazquez:
} Due to the heating of the section by the beam as well as the vacuum } conditions inside the microscope column, the plastic embedment is } undergoing sublimation - similar to what happens during freeze etching. } With removal of the plastic, the remaining osmicated portions of the } section will increase in contrast. In addition, the plastic is stabilized } in the beam so that drifting is minimized. This phenomenon is so beneficial } that some of the newer microscopes have a special feature that } automatically traverses the beam over the section to stabilize the section } and etch away plastic.
Electron irradiation damage (radiolysis) is more important than beam heating. The electron beam cleaves bonds in organic materials, often producing volatile products that are removed by the vacuum. Some polymers (e.g. polystyrene, PS) do not lose much mass but are crosslinked when the radiolysis products react with the polymer. Other polymers (e.g. polymethylmethacrylate, PMMA) can actually "unzip" to monomer after an initial cleavage of the chain by the beam. PMMA loses most of it's mass during heavy irradiation. Most embedding resins are intermediate between the extremes of PS and PMMA. The classic reference explaining the chemistry and physics of irradiation damage of polymers is D. T. Grubb, "Radiation damage and electron microscopy of organic polymers," J. Mat. Sci., 9, 1715-1736 (1974).
Greetings, At the risk of straying slightly off topic, a question was raised in this thread:
} RESPONSE: } Due to the heating of the section by the beam as well as the vacuum } conditions inside the microscope column, the plastic embedment is } undergoing sublimation - similar to what happens during freeze etching. } With removal of the plastic, the remaining osmicated portions of the } section will increase in contrast. In addition, the plastic is stabilized } in the beam so that drifting is minimized... } A much more perplexing phenomenon, however, is why } pizza does taste better the next day. Perhaps some in-depth research is in
I believe the, shall we say, changes in taste in leftover pizza arise from slow extraction of flavor molecules from inside cells to the oil or water surroundings. As spices are commonly bits of plant tissue, the cell walls are a barrier to extraction, and evidently cooking does not always drive the process to saturation. Real info about this can probably be found in one of Hal McGee's books (eg, On Food and Cooking).
Sorry to stray from the topic; please don't flame me, its almost lunch time. Tobias Baskin
Fellow microscopists, I am unable to answer my friend's questions on microwave fixing and embedding of biological specimens, therefore, I am turning to you for help. Is microwave fixing and embedding applicable to all biological specimens? Has this technique been adopted for processing biological specimens routinely by those who had used it before? What are the pros and cons of this technique? If this topic has come up earlier, can someone send me a copy of the summary? Thank you.
Ann Fook Yang Agriculture Canada C.E.F. Ottawa, On K1A 0C6 Canada
} } Hi Everyone, } } Does anyone have any ideas on how to combine data } from Electron microscopy reconstruction and X-ray } crystallography in image analysis when the complex } structure obtained from EM and some components' } structure came from CRYSTALLOGRAPHY? } Dear Jinghua, You could try to calculate the contribution to your image from the x-ray info using a simulation program (NCEMSS is available from Mike O'Keefe). If you subtract that contribution, perhaps image reconstruction will be better, but, since the x-ray data are better than the reconstruction, there might not be much effect. That is, the reconstruction of anything which is not crystalline will be about the same whether or not you subtract the known, crystalline, part. If the complex structure itself is crystalline, a better idea is to try electron crystallography using molecular replacement and other direct methods (including getting phases from the image) to phase the data; then you can reconstruct the specimen from the diffraction data. Good luck. Yours, Bill Tivol
As you remmebe,r immediately after I donated our old Philips 300 TEM last year I identified the need for similar instrument by the Instituto Oncologico Regional del Cibao in the Dominican Republic. The medical director of this non-profit medical facility still needs on an old TEM (by the way the TEM will be operated by S Bencosme, whom you may recalled pulished several papers in the early 60's about the nucleolar organizer region). The institute recently received a small donation and is able to offer a small amount of money for an old instrument in working condition. Any news, respond directly to me please; or inquire if you need information on the institute's non-profit service status.
****************************************************************** *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services * ******************************************************************
1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix plant and animal tissues. In addition, several reports show the benefits of using microwave fixation to preserve soft tissues encased by hard shells (e.g., clams, teeth, insects).
Recommended reading: Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation techniques in pathology to neuroscience studies: A review. J Neurosci Methods, 55, 173-182. Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for microscopy. A review of research and clinical applications: 1970-1992. Prog Histochem Cytochem, 27/4, 1-127. Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd ed.). Leyden: Coulomb Press.
2) Microwave curing of resins (acrylics and epon substitutes) is also successful. Recommended reading: Giammara, B. (1993). Microwave embedment for light and electron microscopy using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87. Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical Guide for Microscopists. Boston: Beth Israel Hospital.
3) Microwave processing is used by some large labs for rapid throughput of specimens. To date, no automatic microwave processors exist so- although microwave processing is rapid it is also a bit labor intensive. Recommended reading: Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large throughput histopathology laboratory. Pathol, 23, 271-273. Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories. Scanning, 15, 88-98.
4) The major problems reported with microwave methods are lack of uniformity and reproduciblity. ALL microwave ovens have high and low energy areas. The literature reports several methods for calibrating microwave ovens to improve results.
Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical Guide for Microscopists. Boston: Beth Israel Hospital. Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: understanding the variables to achieve rapid reproducible results. Microsc Res Tech, 32, 246-254. Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994). Microwave fixation, antigen retrieval and accelerated immunocytochemistry. Cell Vision, 1(1), 76-77.
5) Fortunately, many companies which sell Microscopy products have also designed, tested, and now sell microwave devices and accessories which improve microwave methods and most importantly make them safer to use in the laboratory.
Please contact me if you have additional questions.
Gary R. Login, D.M.D., D.M.Sc. Assistant Professor of Oral Pathology Beth Israel Hospital Department of Pathology 330 Brookline Avenue Boston, Massachusetts, 02215
We are in need of a bell jar for our Varian, Mikros Vacuum Evaporator and don't have $300 for a new one. Diameter 10 1/4 inch or 26 cm Height 1 1/2 in or 29 cm (but could be 30.5 cm).
Anyone having one that doesn't need it, please let me know. Respond directly to my e mail address. Thanks Judy Murphy
Judy Murphy, PhD Dept. of Microscopy San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207
For those of you interested in experimenting, I have found a source of foils 0.025mm thick of the following cobalt compounds: C069/B12/Si12/Fe4/Mo2/Nil Co66/Si16/B12/Fe4/Mo2 Co66/Si15/B14/Fe4/Nil Steven Slap, Vice-President Energy Beam Sciences, Inc.
Dear all, you can try ASTIMEX Scientific Ltd, mount MINM25-53 with cobaltite CoAsS ( S- 20.21%, Fe-7.61, Co-21.63, Ni-7.07 and As 43.47 ). Cheers, Wis Jablonski at W.Jablonski-at-csl.utas.edu.au
"Re: EM & x-ray xtallog" (Oct 30, 2:31pm) References: {199510301931.OAA23469-at-wadsworth.ph.albany.edu} X-Mailer: Z-Mail (3.2.0 26oct94 MediaMail) To: William Tivol {tivol-at-wadsworth.org}
Dear Bill, Thank you for your information! Thinking about the simulation of EM data from X-ray data, is there any difference between the density map calculated seperatedly from EM and X-ray data? Is there any function could be used to calculate the atomic scattering factors for elcetrons?
Although this question does not really concern microscopy, I believe it will be of interest to physics and materials science subscribers.
The question was prompted by the correspondence between Jinghua Tang and Bill Tivol. This involves combining structure data from x-ray diffraction (such as radial density information) with TEM structural data from electron microscopy reconstruction.
My problem may be similar in that it involves an amorphous material (based on XRD) which shows some fringe-like contrast in areas about 2-3 nm in diameter. One way to compare this with similar amorphous materials would be to obtain the structure factor S(Q) from XRD. I have found a nice discussion of determination of S(Q) from conventional XRD in the book by Klug and Alexander. However, I am a bit daunted at the complexity. I am wondering whether any software (preferably shareware) exists which calculates S(Q), prompting the user for the various inputs (sample density, composition, geometry, incident beam, etc.).
I would be grateful for any information leading to such software, or any other comments on this endeavor.
Wharton Sinkler sinkler-at-dvibm3.gkss.de
GKSS Forschungszentrum Abteilung WA D-21502 Geesthacht Germany
A good overview of microwave fixation techniques can be found in the Microwave Cookbook for Microscopists by Drs. Kok & Boon, Coulomb Press, Lyden, 1992, which is available in Canada from Marivac. More recent articles by Gary Login are also well worth looking at- please contact me directly for references. General information can also be found at our home page- http://www.mwrn.com/ebs/ebs.htm Steven E. Slap, Vice-President Energy Beam Sciences, Inc.
"Dear all, you can try ASTIMEX Scientific Ltd, mount MINM25-53 with cobaltite CoAsS ( S- 20.21%, Fe-7.61, Co-21.63, Ni-7.07 and As 43.47). Cheers, Wis Jablonski at W.Jablonski-at-csl.utas.edu.au"
This is indeed true, as I believe was already pointed out by Dr. Charles Garber. The Astimex standard is available both from SPI Supplies and Energy Beam Sciences. My point was just that the Astimex standard contains 53 minerals and costs more than $2000, so, if someone simply wants to experiment with various cobalt compounds, they might prefer other options. Steven Slap, Vice-President Energy Beam Sciences, Inc.
I think that this is a case where sophisticated analyses are not required. Provided that the microscope is not misaligned so we can ignore astigmatism and beam tilt, it is a standard result that HREM is more sensitive to local crystallinity than x-ray diffraction. What you describe is fairly common; the x-ray powder pattern appears to show little to no structure due to Debye-Scherrer broadening with nanoscale crystalline regions.
In other words, this is a partialy crystalline or nanocrystalline material, not an amorphous one.
My posting about no political messages was in response to the message entitled...
RE: CANADIAN PROFESSORS STRIKE FOR ACADEMIC FREEDOM
Not all of you got copies of it as I managed to catch it (by dumb luck) before it went to the entire list. I just happend to be logged in when the initial message came through and stopped it before it was distributed to the whole list.
Clearly messages like this have no place on the Microscopy List.
With respect to a comparison of the density map from x-ray and electron diffraction data, remember that this comparison is only valid in the limit where electron diffraction can be considered as kinematical. With this taken into account, x-ray scattering factors (via the Mott formula for conversion) and electron scattering factors give identical results, except perhaps at very low angles and/or at the very precise level of fractions of a percent (e.g. see some of John Spence's work). Many multislice programs (and probably also Bloch-Wave programs) use either type of scattering factors with ease.
At least at the visual level, we once compared a (kinematical) electron diffraction Patterson function with someone else's x-ray Patterson function and they appeared to be the same.
We faced the problem of replacing Lion-S oil in the diffusion pump of our Hitachi S520 SEM several years ago. At that time, I investigated the matter, and was informed that Lion-S oil is di-(2-ethylhexyl) sebacate, a diester oil that was developed by CVC Products, Inc (525 Lee Road, P.O. Box 1886, Rochester, NY; Ph: 800-448-5900; Fx: 716-458-0424) as one of the first synthetic diffusion pump oils to come into use (see Sect. 5.4.2 of my book, "Vacuum Methods in Electron Microscopy"), and marketed by them under the trade name OCTOIL-S (CVC Stock No: 60786-0031). The same material can also be purchased from Kurt J. Lesker, Inc. (1515 Worthington Ave, Clairton, PA 15025; Ph: 800-245-1656; Fx:41`2-233-4275) under the trade name DIFFOIL-S (Stock No. DIFFOILSBB). The price is about $70 for 500 CC. There are undoubtedly numerous other sources, but these are the first two I came up with - no prejudice intended, just trying to be helpful. W. C. Bigelow (bigelow-at-umich.edu)
Dear Wharton, This sounds like the same phenomenon as seen using holey carbon films to check the astigmatism and resolution. If so, the fringe depends on the focus value and lens parameters, and trying to derive structural information from the fringe would seem to be extrordinarily complex, if possible at all. Yours, Bill Tivol
Although not from a company this looks much like a shameless commercial to seel this guy's books, what do you think?
} 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix } plant } and animal tissues. In addition, several reports show the benefits of using } microwave fixation to preserve soft tissues encased by hard shells (e.g., } clams, } teeth, insects). } } Recommended reading: } Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation } techniques in pathology to neuroscience studies: A review. J Neurosci } Methods, 55, 173-182. } Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for } microscopy. A review of research and clinical applications: 1970-1992. Prog } Histochem Cytochem, 27/4, 1-127. } Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd } ed.). Leyden: Coulomb Press. } } } 2) Microwave curing of resins (acrylics and epon substitutes) is also } successful. } Recommended reading: } Giammara, B. (1993). Microwave embedment for light and electron microscopy } using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87. } Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical } Guide for Microscopists. Boston: Beth Israel Hospital. } } } 3) Microwave processing is used by some large labs for rapid throughput of } specimens. To date, no automatic microwave processors exist so- although } microwave processing is rapid it is also a bit labor intensive. } Recommended reading: } Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large } throughput histopathology laboratory. Pathol, 23, 271-273. } Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories. } Scanning, 15, 88-98. } } } 4) The major problems reported with microwave methods are lack of } uniformity and } reproduciblity. ALL microwave ovens have high and low energy areas. The } literature reports several methods for calibrating microwave ovens to improve } results. } } Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical } Guide for Microscopists. Boston: Beth Israel Hospital. } Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: } understanding } the variables to achieve rapid reproducible results. Microsc Res Tech, 32, } 246-254. } Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994). } Microwave fixation, antigen retrieval and accelerated immunocytochemistry. } Cell Vision, 1(1), 76-77. } } 5) Fortunately, many companies which sell Microscopy products have also } designed, tested, and now sell microwave devices and accessories which improve } microwave methods and most importantly make them safer to use in the } laboratory. } } } Please contact me if you have additional questions. } } Gary R. Login, D.M.D., D.M.Sc. } Assistant Professor of Oral Pathology } Beth Israel Hospital } Department of Pathology } 330 Brookline Avenue } Boston, Massachusetts, 02215 } } glogin-at- bih.harvard.edu } Telephone: 617-667-2034 } Fax: 617-667-8676
John Mansfield North Campus Electron Microbeam Analysis Laboratory 413 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (313)936-3352 FAX (313)936-3352 jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu or jfmjfm-at-umich.edu they all reach me! URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html
Thanks very much to those people who replied to my request for infomation about freeze slamming cultured cells.
I have had a question passed on to me about BioRad immunogold starter kits. These starter kits provided material for an introduction to EM immunocytochemistry. They were aimed as an introduction to researchers and as a convenient and economically priced kit for classroom teaching.
Does anyone know what company ended up with the BioRad Gold part of the Microscience division when it was split up?
Does anyone know of any other immunocytochemical supplier who supplies "starter kits"?
Regards,
Richard E
Richard Easingwood South Campus Electron Microscope Unit Otago Medical School PO Box 913 Dunedin NEW ZEALAND
Does anyone have a source for racks (metal, plastic...) that will hold glass coverslips for staining? Several of us have used metal racks in the past that would hold at least 10 coverslips - now we can only find smaller ones in the catalogues. Preferably for 22x22mm cs, but we'll take anything anyone sells! Thanks! Tamara Howard Cold Spring Harbor Lab howard-at-cshl.org
THE QUESTION WAS ASKED EARLIER: } Although not from a company this looks much like a shameless commercial to } seel this guy's books, what do you think?
MY REACTION: Having just reviewed the area of microwave fixation, I can tell you that there are 4-5 major contributors who have been involved in the development of this technology from the very beginning. Dr. Login is one of the major contributors in this area and he has referenced the significant contributions appropriate for the question posted earlier by someone just starting out with microwave fixation.
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
The Engineering Department at the University of Denver would like to acquire a used field emission SEM with an Energy Dispersive X-Ray Analysis System. An Hitachi model S800 would fit our needs precisely.
The Engineering Department at DU offers ABET accredited BS and MS degrees in Mechanical and Electrical Engineering, and a PhD in Material Science. Our current enrollment includes about 110 undergrads, and 21 grad students.
Our Material Science laboratory, operated by Dr. Paul Predecki, is equipped with fairly modern x-ray diffraction equipment. However, our existing SEM has not responded to efforts at resuscitation, and has become a fiscal black hole.
As a private non-profit we are limited in the financial resources available to us. We have, however, set aside some funds to assist with the SEM acquisition.
Please contact me directly if you have any leads. I can be reached at jgetty-at-du.edu, or (303)871-3129.
Thanks,
John Getty Laboratory Director, Engineering University of Denver Denver, CO 80208
I see no reason for Dr. Login not to be able to cite the key references in the field even tho he co-authored them. I think he should be commended for taking the time and pointing them out to the individual. I passed the list on to one of my students who had recently expressed interest in playing with microwave techniques. You don't have to buy his book - you can simply go to the library. I also note he cites primary references in the literature and the main competitor to his book (the microwave cookbook). Its negative comments like this that turn people off from replying to simple queries. For the record, I don't have any financial interest in any of the publishers and I am not a friend of Dr. Login. I did meet him at a meeting once and we had a pleasant 15 min discussion in which he happily gave me some hints about a microwave technique I was trying.
} Although not from a company this looks much like a shameless commercial to } seel this guy's books, what do you think? } } } 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix } } plant } } and animal tissues. In addition, several reports show the benefits of using } } microwave fixation to preserve soft tissues encased by hard shells (e.g., } } clams, } } teeth, insects). } } } } Recommended reading: } } Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation } } techniques in pathology to neuroscience studies: A review. J Neurosci } } Methods, 55, 173-182. } } Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for } } microscopy. A review of research and clinical applications: 1970-1992. Prog } } Histochem Cytochem, 27/4, 1-127. } } Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd } } ed.). Leyden: Coulomb Press. } } } } } } 2) Microwave curing of resins (acrylics and epon substitutes) is also } } successful. } } Recommended reading: } } Giammara, B. (1993). Microwave embedment for light and electron microscopy } } using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87. } } Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical } } Guide for Microscopists. Boston: Beth Israel Hospital. } } } } } } 3) Microwave processing is used by some large labs for rapid throughput of } } specimens. To date, no automatic microwave processors exist so- although } } microwave processing is rapid it is also a bit labor intensive. } } Recommended reading: } } Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large } } throughput histopathology laboratory. Pathol, 23, 271-273. } } Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories. } } Scanning, 15, 88-98. } } } } } } 4) The major problems reported with microwave methods are lack of } } uniformity and } } reproduciblity. ALL microwave ovens have high and low energy areas. The } } literature reports several methods for calibrating microwave ovens to improve } } results. } } } } Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical } } Guide for Microscopists. Boston: Beth Israel Hospital. } } Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: } } understanding } } the variables to achieve rapid reproducible results. Microsc Res Tech, 32, } } 246-254. } } Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994). } } Microwave fixation, antigen retrieval and accelerated immunocytochemistry. } } Cell Vision, 1(1), 76-77. } } } } 5) Fortunately, many companies which sell Microscopy products have also } } designed, tested, and now sell microwave devices and accessories which improve } } microwave methods and most importantly make them safer to use in the } } laboratory. } } } } } } Please contact me if you have additional questions. } } } } Gary R. Login, D.M.D., D.M.Sc. } } Assistant Professor of Oral Pathology } } Beth Israel Hospital } } Department of Pathology } } 330 Brookline Avenue } } Boston, Massachusetts, 02215 } } } } glogin-at- bih.harvard.edu } } Telephone: 617-667-2034 } } Fax: 617-667-8676 } } John Mansfield } North Campus Electron Microbeam Analysis Laboratory } 413 SRB, University of Michigan } 2455 Hayward, Ann Arbor MI 48109-2143 } Phone: (313)936-3352 FAX (313)936-3352 } jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu } or jfmjfm-at-umich.edu they all reach me! } URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
In response to John Mansfield's recent comment about commercialism on the list server.
My E-mail on Microwave Fixation (Oct 30th) was sent in response Dr. Yang's queries about microwave fixation posted on the microscopy list server (Oct 30th). I did not repeat Dr. Yang's message in my E-mail. IN the future I will make reference to or include the message I am replying to to avoid confusion.
John Mansfield's E-mail, Dr. Yangs' E-mail, and my E-mail follow for the sake of sending a complete message. ------------------------------
Although not from a company this looks much like a shameless commercial to seel this guy's books, what do you think?
----------------
Fellow microscopists, I am unable to answer my friend's questions on microwave fixing and embedding of biological specimens, therefore, I am turning to you for help. Is microwave fixing and embedding applicable to all biological specimens? Has this technique been adopted for processing biological specimens routinely by those who had used it before? What are the pros and cons of this technique? If this topic has come up earlier, can someone send me a copy of the summary? Thank you.
Ann Fook Yang Agriculture Canada C.E.F. Ottawa, On K1A 0C6 Canada ------------------------
1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix plant and animal tissues. In addition, several reports show the benefits of using microwave fixation to preserve soft tissues encased by hard shells (e.g., clams, teeth, insects).
Recommended reading: Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation techniques in pathology to neuroscience studies: A review. J Neurosci Methods, 55, 173-182. Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for microscopy. A review of research and clinical applications: 1970-1992. Prog Histochem Cytochem, 27/4, 1-127. Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd ed.). Leyden: Coulomb Press.
2) Microwave curing of resins (acrylics and epon substitutes) is also successful. Recommended reading: Giammara, B. (1993). Microwave embedment for light and electron microscopy using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87. Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical Guide for Microscopists. Boston: Beth Israel Hospital.
3) Microwave processing is used by some large labs for rapid throughput of specimens. To date, no automatic microwave processors exist so- although microwave processing is rapid it is also a bit labor intensive. Recommended reading: Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large throughput histopathology laboratory. Pathol, 23, 271-273. Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories. Scanning, 15, 88-98.
4) The major problems reported with microwave methods are lack of uniformity and reproduciblity. ALL microwave ovens have high and low energy areas. The literature reports several methods for calibrating microwave ovens to improve results.
Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical Guide for Microscopists. Boston: Beth Israel Hospital. Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: understanding the variables to achieve rapid reproducible results. Microsc Res Tech, 32, 246-254. Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994). Microwave fixation, antigen retrieval and accelerated immunocytochemistry. Cell Vision, 1(1), 76-77.
5) Fortunately, many companies which sell Microscopy products have also designed, tested, and now sell microwave devices and accessories which improve microwave methods and most importantly make them safer to use in the laboratory.
Please contact me if you have additional questions.
Gary R. Login, D.M.D., D.M.Sc. Assistant Professor of Oral Pathology Beth Israel Hospital Department of Pathology 330 Brookline Avenue Boston, Massachusetts, 02215
Gary R. Login, D.M.D., D.M.Sc. Assistant Professor of Oral Pathology Beth Israel Hospital Department of Pathology 330 Brookline Avenue Boston, Massachusetts, 02215