Hello, We are currently doing diffraction on our TEM for school. Nobody seems to know what the standard exposure time is when taking a photo of the diffraction pattern. I would appreciate any suggestions.
Thank you in advance Leah L. Dobbs Madison Area Technical College
} } About magnetic fields measurments, we have heard of an Australian company } called "the Arlundya division of the Dindima group", but we failed in } finding their present phones and or www address. } } They seem to be in Victoria, Australia. Can anyone help? } } Thank you in advance, } } Yves MANIETTE } Universitat de Barcelona } Serveis Cientifico Tecnics } Unitat ESCA TEM } Carrer Lluis Sole i Sabaris, 1-3 } E-08028 BARCELONA ESPANYA } } Tel +34 (9)3 402 16 95 } Fax +34 (9)3 402 13 98 } Hi Yves.
I looked up the local telephone book and found the following address and phone number that might be what you are looking for.
THE DINDIMA GROUP P/L 10 Argent Place RINGWOOD, Victoria, 3134 Australia Telephone Number +61 3 9873 4455
Regards
Hans Brinkies SWINBURNE, University of Technology School of Mechanical and Manufacturing Engineering Industrial Microscopy HAWTHORN, Victoria, 3122 Australia
} } About magnetic fields measurments, we have heard of an Australian company } called "the Arlundya division of the Dindima group", but we failed in } finding their present phones and or www address. } } They seem to be in Victoria, Australia. Can anyone help? } } Thank you in advance, } } Yves MANIETTE } Universitat de Barcelona } Serveis Cientifico Tecnics } Unitat ESCA TEM } Carrer Lluis Sole i Sabaris, 1-3 } E-08028 BARCELONA ESPANYA } } Tel +34 (9)3 402 16 95 } Fax +34 (9)3 402 13 98 } } Hi Yves.
I looked up the local telephone book and found the following address and phone number that might be what you are looking for.
THE DINDIMA GROUP P/L 10 Argent Place RINGWOOG, Victoria, 3134 Australia Telephone Number +61 3 9873 4455
Regards
Hans Brinkies SWINBURNE, University of Technology School of Mechanical and Manufacturing Engineering Industrial Microscopy HAWTHORN, Victroria, 3122 Australia
To follow up in the discussion on diffraction pattern exposure time. For normal diffraction patterns I use, as a general rule of thumb, 1/5th to 1/10th of the time given by the microscope. E.g. If the exposure time given by the microscope would be 30 seconds, I will expose the film approximately 3 - 5 seconds. This is giving me good results, most diffraction spots can be seen and the central / transmitted beam is not too bright. Of course if you need to see more details in the diffraction pattern one needs to experiment.
} We are an electron microscopy group and we search for a new CCDcamera, to } scan our electron diffraction films properly. We got your address from } George Farrants from Calidris. Could you hire us some advice which } CCD-camera is best. We would like to buy an advanced CCD camera with 16 } Bit } Dear Hans, One caution about using a CCD to scan film is that stray light can be picked up as arising from other pixels. This results in higher light levels (lower OD) being recorded than actually is there. This is particu- larly troublesome for the very intense spots. Remember, a spot with OD = 4 has only 1/100 of a % of the incident light transmitted, so very small amounts of stray light will cause large errors. The most accurate method of scanning film uses an instrument which has a small beam and a small detec- tor slit, such as the Perkin-Elmer, Optronics or others with which I am not familiar. This accuracy for the intense spots may not be necessary for your application, and using a scanning microdensitometer takes a long time. Good luck. Yours, Bill Tivol
[snips] } } We are currently doing diffraction on our TEM for school. Nobody seems to } } know what the standard exposure time is when taking a photo of the } } diffraction pattern. } } The short answer is that there isn't a standard exposure time. } True, the best exposure time depends on the specimen, the use to which the data will be put and the method used to extract the data from the film. Obviously, the more intense the pattern, the shorter the ex- posure. If all that is of interest is measurement of the lattice para- meters, you want an exposure which gives as many accurate spot positions as possible. If you want accurate intensity measurements, you may want to keep the exposure short enough so that the spot OD's are in the linear range of the film's response. (An alternative is to calibrate the expo- sures with a blackening curve.) As pointed out by others, this may require several exposures. If you are measuring the film with a scanning micro- densitometer, you can have up to OD = 4 and not lose too much accuracy, but if you use a CCD, you have to lower the exposure to the point where stray light does not cause errors measuring the more intense spots.
} Factors to think about are: } } 2. The type of electron diffraction pattern and type of specimen will have } a big influence. Traditional SADP from thin specimens results in } diffraction patterns with a huge dynamic range. In this case it is probably } impossible to get all the information in a single negative - a short } exposure will capture the intense spots but a longer exposure will be } needed to get any faint spots.
Using LoDose film and a Perkin-Elmer 1010A microdensitometer at a window setting of 10 micron square, I have been able to recover both the low-order spots (OD up to ~4) and faint spots (not visible to the eye, but they integrate to a positive value; background and systematically absent reflections are either negative or within statistics of 0). } } 3. What you want from the pattern will also affect how you photograph it. } You will probably need several images to collect all the information you } need.
In addition, it can be useful to have a sequence of patterns from one area so that you can see if the crystal is being degraded in the beam. If necessary, you can extrapolate the measurements to 0 dose. } } 4. You may also need to think about how you process the images. It sounds } as though you are using a traditional 'wet' photographic route. One problem } here it that printing paper has less dynamic range in a single exposure } than film. So you can get a negative with lots of information in it, but } then find it impossible to print in a single exposure - this where you have } to go in by hand, with pieces of card (with and without holes). With some } practise, you can expose as many as 3 or 4 different areas of the paper for } different times and end up with a single print that contains all the detail } of the original negative. } If you scan the negative into a computer, you needn't worry about the printing. If you want prints for publication, they can be obtained from computer files. The advantage here is that the files can be mani- pulated (by subtracting background, rescaling various areas, etc.) so that the pertinant info can be readily seen. Of course, all such manipulations must be described in the paper.
} To conclude, even with experience, whatever type of diffraction pattern you } are photographing, you'll probably need at least 2 or 3 exposures to be } confident of getting one right. } I am not confident after 2 or 3 exposures, but I *can* get the right exposure after I see the results of the first 2 or 3. BTW, use at least a factor of 4 between exposures; i.e., 1, 4 and 16 sec, etc. Good luck. Yours, Bill Tivol
What Jacob is referring to is a software package called ImgLib devoloped at Lawrence Berkeley Lab to help organize and browse digital images via a Web browser. The relevant URL is http://imglib.lbl.gov/ImgLib. Check out the "For documentation or to download software" button for a description of what it does. Try "Introduction" first. The "Index to all the collections" button lets you try out the searching/browsing interface. The Berkely Lab collection, and LungStructure are accessible without a password.
The executive summary is: Server runs on a Unix system, currently Solaris 2.5 and mostly consists of Perl scripts called by the HTTP deamon. It could be fairly easily ported to other Unix systems. It also uses several portable image processing libraries which would make it harder to port to Windows or MacOS. The client side is just a Web browser. It is optimized a bit for Netscape. The code is downloadable from our Web site. If you actually want to copy it and get it running on your Unix server, I will probably have to help you a bit, though there is a README file included in the tar file.
Mary
- --------------------------------------------------------------------- Mary R. Thompson work - {MRThompson-at-lbl.gov} Imaging & Distributed Computing Group (510) 486-7408 Lawrence Berkeley National Lab home - thompson-at-dnai.com (510) 540-6538 - ----------------------------------------------------------------------
I apparently gave the wrong FAX number for Linear Research Associates in my last listserver memo on magnetic fields in EM labs. The following corrective message, addressed to me, is self explainatory: - - - - - - - - - - - - - - - - - - - Dr. Bigelow,
Don Grimes of Microscopy Today has forwarded to my attention your recent microscopy listserv memo on the interesting topic of ubiquitous, annoying magnetic fields. In your memo, you refer to my recent article series in MT on interfering magnetic fields, which I certainly appreciate. I am writing to mention that the fax number you cite in your memo is unfortunately not correct. The actual fax number for Linear Research Associates is 607-387-7806 (v: 607-387-3411). We will of course be glad to provide further descriptive materials for anyone interested in our systems, services or research work in the area of active-feedback magnetic field mitigation.
id IAA01027; Tue, 26 Nov 1996 08:32:03 -0600 Received: from emiris.iaf.uiowa.edu by ns-mx.uiowa.edu (8.7.5/19950309.1) on Tue, 26 Nov 1996 08:33:30 -0600 id IAA31406 with SMTP Received: from ns-mx.uiowa.edu (ns-mx.uiowa.edu [128.255.1.3]) by Sparc5.Microsc opy.Com (8.6.11/8.6.11) with ESMTP id IAA05144 for {Microscopy-at-Sparc5.Microscopy .Com} ; Tue, 26 Nov 1996 08:22:42 -0600 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id IA A05147 for dist-Microscopy; Tue, 26 Nov 1996 08:22:43 -0600 Received: from Sparc5.Microscopy.Com (sparc5.microscopy.com [206.69.208.10]) by ormail.intel.com (8.8.3/8.7.3) with SMTP id PAA06520; Tue, 26 Nov 1996 15:48:52 -0800 (PST) Received: from ormail.intel.com by relay.hf.intel.com with smtp (Smail3.1.28.1 #2) id m0vSXI9-000qDPC; Tue, 26 Nov 96 15:51 PST
Message-Id: {9612022353.AA1960-at-pho903.sbphrd.com} To: microscopy {microscopy-at-Sparc5.Microscopy.Com} {Beverly_E_Maleeff-at-sbphrd.com}
PHILADELPHIA SOCIETY FOR MICROSCOPY
DECEMBER 1996 MEETING NOTICE Thursday, December 12, 1996
Seeing Polymer Ultrastructure
Matthew R. Libera, Sc.D. Department of Materials Science and Engineering Stevens Institute of Technology Hoboken, NJ
Just as they fill an important niche in the study of inorganic engineering materials, the various techniques of transmission electron microscopy (TEM) play an important role in the study of polymers. Traditional polymer-imaging methods employ heavy-element stains to preferentially label microstructural features. These have contributed substantially to the understanding of polymer structure/property relations, but they are not without significant shortcomings. This presentation will briefly review how staining leads to contrast with examples from homopolymer blends and microphase-separated block copolymers. Ongoing research to develop microstructural mapping techniques based on electron energy-loss spectroscopy will then be discussed. Examples will be presented where contrast can be generated in blends due to spatial variations in both valence (polyethylene/polystyrene) and core (polyethylene/nylon) electron structure. The presentation will conclude with a brief description of a second method - transmission electron holography - which offers an opportunity for phase-contrast imaging of unstained multiphase polymers.
DATE: Tuesday, December 12, 1996
PLACE: Laboratory for the Research of Science and Materials (LRSM) Building, 33rd and Walnut Street, Philadelphia, PA. (On the campus of the University of Pennsylvania) Parking is available behind the LRSM Building after 5:00 PM.
TIME: 5:30 PM Social hour, hosted by our meeting sponsors
6:30 PM Holiday Dinner Members $12.00 Student members $6.00 Non-members $15.00
Menu: Beer, wine, assorted soda and bottled water Munchies
Hickory ham with apricot glaze served with orange halves and cranberry relish Tortellini with broccoli and red peppers Salad of winter greens with dressing Chef's vegetable du jour Rolls and butter
Cherry topped cheesecake with fresh mint
Coffee, decaf or tea
7:30 PM Speaker
Reservations will be taken by Ms. Pat Overend at the University of Pennsylvania, 215/898-8337. Deadline for reservations will be Tuesday, December 10. If you have any questions regarding the meeting please feel free to contact Rollin Lakis of the Executive Council at 215/898-8718. Cancellations must be received by Ms. Overend no later than 5:00 PM, December 10, 1996.
RESERVATIONS ARE REQUIRED FOR DINNER. We cannot guarantee you a meal if you do not make a reservation by the deadline above.
Could one of you please forward my reply to the list that Jacob mentioned the ImgLib software on.
What Jacob is referring to is a software package called ImgLib devoloped at Lawrence Berkeley Lab to help organize and browse digital images via a Web browser. The relevant URL is http://imglib.lbl.gov/ImgLib. Check out the "For documentation or to download software" button for a description of what it does. Try "Introduction" first. The "Index to all the collections" button lets you try out the searching/browsing interface. The Berkely Lab collection, and LungStructure are accessible without a password.
The executive summary is: Server runs on a Unix system, currently Solaris 2.5 and mostly consists of Perl scripts called by the HTTP deamon. It could be fairly easily ported to other Unix systems. It also uses several portable image processing libraries which would make it harder to port to Windows or MacOS. The client side is just a Web browser. It is optimized a bit for Netscape. The code is downloadable from our Web site. If you actually want to copy it and get it running on your Unix server, I will probably have to help you a bit, though there is a README file included in the tar file.
Mary
--------------------------------------------------------------------- Mary R. Thompson work - {MRThompson-at-lbl.gov} Imaging & Distributed Computing Group (510) 486-7408 Lawrence Berkeley National Lab home - thompson-at-dnai.com (510) 540-6538 ----------------------------------------------------------------------
That topic again! Sorry, I haven't been keeping the information on scanners and now I have to make fast decision on buying one. Is there any good scanner that one can digitize from microscope slides, negatives, autoradiographic film..? If there is one, we have to be able to attach it to a PC and Mac. And also the approximate price, if you know. Thank you,
Lilith Ohannessian-Barry e-mail: lilith barry-at-nrc.ca National Research Council Tel: 613-993-6460 Institute of Biological Sciences Fax: 613-941-4475 Montreal rd Campus, M54 Ottawa, Ont. K1A 0R6 CANADA
In the good old days before electron microscopes had exposure meters, the generally accepted method of getting the right exposure for SAD patterns was to overfocus the illumination until the diffraction spots were only just visible and expose for about 60 seconds. Obviously, "just visible" is a highly subjective condition, but there is considerable latitude with this method and you can usually get away with just one exposure unless the crystal is very thick. Weak reflections usually show up quite well, even ones which are not visible to the eye at normal brightness. Keeping the illumination well overfocussed has the additional advantage of ensuring that the spots are as sharp as possible. Of course the difficulties with printing the patterns remain, but these have already been discussed in this forum.
---------------------- Dr Eric E. Lachowski e.lachowski-at-abdn.ac.uk
Does anyone know where I can purchase aluminium grids for TEM? I do not want ones that have a mesh, but rather ones with just a large hole. The bigger the hole ( {2000=B5m) the better.
Thank you, Peggy
Margaret E. Bisher
NEC Research Institute 4 Independence Way Princeton, NJ 08540. Tel.: (609) 951-2629 =46ax: (609) 951-2496 e-mail: peggy-at-research.nj.nec.com
I need some help in obtaining cryostat sections of undecalcified bone. I am using diffusible tracers and cannot decalcify the bone. Any suggestions would be welcomed on sectioning angle, temperature, knives, etc. The piece that I need to section is about 5-7 mm square and I need to have sections of at least 20 um in thickness.
Dorota Wadowska asks "Can anyone recommend a commercial source of good quality protein A gold (20 nm)."
We get ours from an interesting but reliable source:
The University of Utrecht, Department of Cell Biology, Medical School, AZU HOZ.314 Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
Fax 011 31 30 541 797.
The contact name is George Postuma but the enterprise is run by Jan Slot (famous in colloidal gold and immunocytochemical circles).
Colloidal gold coupled to protein has a short shelf life so their deal is for them to sell you 1 ml of gold probe which they deliver in quarterly installments. Each instalment is delivered soon after it is made so there are no storage problems. I think they will even make customised preparations of colloidal gold.
We have great succes with our probes and get no consideration from our supplier for recommending them.
Best regards
Paul Webster Center for Cell Imaging Yale School of Medicine Come check us out at http://info.med.yale.edu/cellimg
} Date: Tue, 03 Dec 1996 09:47:09 -0400 (AST) } From: Dorota Wadowska {wadowska-at-upei.ca} } To: microscopy-at-Sparc5.Microscopy.Com } Subject: TEM-protein A gold } } Hi there! } Can anyone recommend a commercial source of good quality protein A } gold (20 nm). } Thanks } Dorota } We use Auroprobe (Janssen), marketed here by Amersham. Consistent quality. Always works. Expensive. Some other brands work too--usually have to use them at higher concentrations; thus, may come out just as expensive.
1 800 387 7160
I have no commercial interest in this product--just a satisfied customer.
Other sources: EMS in Pensylvania, EY in California. Can purchase less than 1 ml at a time. Note optical density for comparison with other sources.
Use a smaller a gold if you can. 10 nm is good, if you can get away with that size. 5 nm is a pain--hard to see; have to go up to high mag; is gray like ferritin, not dense black.
Sara
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
Can someone please send me the instructions for SUBSCRIBING, UNSUBSCRIBING, etc. to this Microscopy List? I have tried subscribing by going to the MSA web site but had no luck.
Thanks!
Matthew A. Stough HTML Graduate Fellow Oak Ridge National Laboratory One Bethel Valley Road PO Box 2008 Bldg. 4515 Oak Ridge, TN 37831-6062
Two recent postings have implied colloidal gold probes have a relatively short half life. Could people comment on how long their probes are stable and how they store them. TIA
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (573)-882-4712 (voice) (573)-882-0123 (fax)
Three months tops for me, stored in the original container at 4C, regardless of supplier. Definitely weaker after 6 weeks. (I've tried Amersham, E-Y Labs, Chemicon, Biocell--Chemicon gets my vote for the best buy.)
I have some aged human brain tissue exibiting autofluorescence. I susspect the culprit is lipofuscin. I seem to remember a thread where Chip Montrose asked about lipofusion, but I don't recall if he received any "cures". Does any one know how to block the autofluorescence of lipofusion?
TIA, Kathy Walters
Kathy Walters / / Research Assistant III / /\ Center for Microscopy Research / /\ \ University of Iowa /_/ \ \ 85 EMRB ____ ((O)) Iowa City, Iowa 52242 | | / / || / / email: kwalters-at-emiris.iaf.uiowa.edu ----------- fax: (319)335-8049 ------------- www: http://www.uiowa.edu/~cemrf
I am looking for an old (but operable) evaporative coater for dedicated Au coating. The samples are for ion microprobe analysis and must be evaporatively coated. Any offers should give an idea of price and condition.
Thank you
Brendon J. Griffin Centre for Microscopy and Microanalysis The University of Western Australia Nedlands, WA, AUSTRALIA 6907 ph 61-9-380-2739 fax 61-9-380-1087
The main problem I had when cutting mouse noses (snouts?) was getting the sections to adhere to the slides during processing. My best results came after reading a paper in The Journal of Histochemistry and Cytochemistry. The article is "An Improved Method for Preparing Cryostat Sections of Undecalcified Bone for Multiple Uses." Vol.38, No. 3. pp. 443-448. Good Luck, Kathy
Kathy Walters / / Research Assistant III / /\ Center for Microscopy Research / /\ \ University of Iowa /_/ \ \ 85 EMRB ____ ((O)) Iowa City, Iowa 52242 | | / / || / / email: kwalters-at-emiris.iaf.uiowa.edu ----------- fax: (319)335-8049 ------------- www: http://www.uiowa.edu/~cemrf
On Tue, 3 Dec 1996, Michael J. Lyon wrote:
} I need some help in obtaining cryostat sections of undecalcified bone. I } am using diffusible tracers and cannot decalcify the bone. Any } suggestions would be welcomed on sectioning angle, temperature, knives, } etc. The piece that I need to section is about 5-7 mm square and I need } to have sections of at least 20 um in thickness. } } Thanks in advance } } Mike }
Message-Id: {1.5.4.32.19961204213527.006d9070-at-biotech.ufl.edu} X-Sender: gwe-at-biotech.ufl.edu X-Mailer: Windows Eudora Light Version 1.5.4 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Shelf life depends on the protein conjugated to the gold and the batch. We use IgG conjugates for 6 months or more storing them at 4 C. I have used protein A conjugates that were a year and a half old. Other times they have gone bad much more quickly. It is always best to have a positive control for activity of the gold if it has been several weeks since the last use. If the gold is in a glycerol solution it can be frozen to extend its useful life.
At 12:21 PM 12/4/96 -0500, you wrote: } Two recent postings have implied colloidal gold probes have a relatively } short half life. Could people comment on how long their probes are stable } and how they store them. TIA } } } } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } 3 Tucker Hall } University of Missouri } Columbia, MO 65211 } (573)-882-4712 (voice) } (573)-882-0123 (fax) } } } } ******************************************************* G.W. Erdos, Ph.D. Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/
***** "Many shall run to and fro, and knowledge shall be increased" Daniel 12:4
I have a couple of former EM students who would like to image the distribution of CD4 proteins on the surface of CEM-E5 cells (immortal human helper T-cell line). The cells are grown in a media known as RPMI (I was informed that it's similar to the Modified Eagle Medium) and supplemented with a bovine growth serum.
They have access to a primary antibody, OkT4, which is specific for the CD4 protein and a secondary gold labeled antibody - mouse IgG + IgM with a 12nm gold bead.
Since I have not had much experience with immuno-EM, I would appreciate any direction, references and advice you might have.
The specific questions I have follow:
1. Does this sound like a feasible project - can SEM be used to image such membrane proteins as CD4 using gold labeled abs? (I have a tungsten based Hitachi S-2400 SEM with a 4nm resolving power at 25kv - secondary electron detector only).
2. Would a BSE detector be more useful in imaging the gold labeled abs?
3. Can you recommend a protocol (or specific reference) for fixing and processing the cells - Is there something we could use to affix the cells for processing - would polylysine coated coverslips be useful?
4. For drying, would CPD work well or would you recommend the organo-silicon route (TMS, HMDS) directly from ethanol dehydration series?
5. I would assume that gold sputter coating would be contrary to our goals - Is there a method for producing a high quality image at lower voltages OR a method for enhancing conductivity without the need for conductive coating [I am aware of the OTO(TO) technique for more "conventional" samples].
6. In a related project, these students would like to study the relationship between CD4 and fusin receptors on these cells. Could these sites be labeled differentially so as to be discerned when imaged? In addition, are antibodies to fusin currently available.
7. Are there any additional points I haven't considered - once again, I'm rather new to this technique!
Thanks in advance!
Stephen J. Beck Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: {becks-at-sunynassau.edu URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
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Hi,
Are there any programs (PC base preferred) which will do EDS analysis of a thin film on a bulk substrate? Typical we look at ~1000 A thick films (sometimes multilayer films) on silicon substrates. I have heard of a program call STRATA, will this program do what I require?
I know there is the GMRFILM program in the archives, however the zip file appears to be corrupted.
Many Thanks,
Keith.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr. K. Moulding.
Materials Characterisation and Preparation Centre, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
MeetingName: IAS Holiday Meeting/Pittsburgh MeetingDates: Dec. 12, 1996 MeetingTopic: Two presentations will be given: John Friel, Princeton Gamma Tech,on " Low-Voltage, High-Resolution X-ray Mapping", and Louis Hector, Alcoa, on " Atomic Force Mircoscopy as a Tool in Materials Science".
The IAS is a professional society providing a forum for the exchange of information related to materials characteriztion in fields such as metallurgy, chemistry, microscopy, life science, and biotechnology.
MeetingSponsor: Instrumentation and Analysis Society (IAS) City: Alcoa Technical Center State: PA Zip: 15069 Country: Westmoreland Interests: Physical Sciences, Scanning Probe, SEM ContactName: Liz Goodwin or Hasso Weiland ContactCity: Alcoa Center ContactState: PA ContactZip: 15068 Phone: 412 744-100 or 412 337-3133 email: goodwin-at-sgi.net or weiland_h-at-atc.alcoa.com ---------------------------------------------------------------------------
G'Day. Does anyone have any information regarding neurogenesis in the subventricular zone? I have a project due and I can't find any up to date information. I have micrographs from the SEM, but I am unsure what exactly I am looking at.
Thanks in advance for anyone who can help me.
Christopher L.C. Wang Dept. of Bio. Sci. University of Lethbridge Tel: (403) 329-2210
We use Aurion. It is the name of a Belgian society managed by Jan Leunissen. Jan Leunissen, Ph.D. AURION ImmunoGold Reagents & Accessories Costerweg 5, 6702 AA Wageningen The Netherlands
phone (31)-317-497676 fax (31)-317-415955 I think that the Aurion products are commercialised by EMS in the USA. I have no commercial interest in the gold conjugates they are selling, I am just satisfied, not only by the products but also by the advices and discussions.
We use gold probes from Jackson exclusivly. Stored at 4C, we get excellent results for at least one year -- I have one batch of anti-HRP gold that is three years old and still works fine (by eye). I have not done a quantitative analysis on decrease in labeling over time. We also see a batch-to-batch variation in shelf life.
Greg Martin Dept. of Cell Biology and Anatomy Johns Hopkins School of Medicine
I'm not exactly sure of what you're looking for, but the following paper may prove interesting even though the study was done in fish:
Zupanc, Gunther K.H., and Horschke, Ingrid (1995) Proliferation zones in the brain of adult Gymnotiform fish: A quantitative mapping study. J.Comp.Neurol. 353:213-233. .
Help Please, Does anyone know of a way to recover writing from Histo tissue holders that have been processed in Carnoy's? Details as follows: Holders were marked with, Securline MarkerII/Superfrost (permanent, solvent-resistant) SP Cat No P1220, then placed in Carnoy's fixative, (Absolute alcohol, Chloroform and glacial acetic acid.) All of the writing seems to have dissolved. Are there any forensic scientists or others with experience that may help us to recover some very valuable tissue? All suggestions are most welcome. Thanks Linda Fox Loyola University Medical Center lfox1-at-wpo.it.luc.edu, phone 1-708-216-3395, fax 1-708-216-3913
The BioSite colloidal gold probes which we sell will keep for about a year in the refrigerator, and for an indefinite period of time when frozen. Most of our customers will aliquot the reagent, and store the small volumes in the freezer until needed. The probes are available in 1ml and .25ml volumes.
Best regards, Steven E. Slap, Vice-President ******************************** Energy Beam Sciences, Inc. Adding Brilliance To Your Vision ebs-at-ebsciences.com http://www.ebsciences.com/ ********************************
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OPTICAL MICROSCOPIST POSITION:
Immediate opening for Assistant-in or Assistant Scientist to operate and maintain confocal and deconvolution microscopes in Center for Structural Biology. This position will involve user support and collaborative research with investigators from various departments. Final rank and starting salary are commensurate with education and experience. Candidates should have M.S. or Ph.D. with experience, and be knowledgable in use of advanced optical microscopies, and in their application to biological and medical research. Anticipated starting date February 1, 1997. Send CV by January 20, 1997 to Dr. Thomas H. Mareci, Center for Structural Biology, PO Box 100245, University of Florida, Gainesville, FL 32610. The University is an equal employment opportunity/affirmative action employer.
Does anyone have any information regarding neurogenesis in the subventricular zone? I have a project due and I can't find any up to date information. I have micrographs from the SEM, but I am unsure what exactly I am looking at.
Thanks in advance for anyone who can help me.
Christopher L.C. Wang Dept. of Bio. Sci. University of Lethbridge Tel: (403) 329-2210
Kramercy and Sealock (J. Histochem and Cytochem, 1991, Vol. 39 p. 37-39) investigated the presence of unconjugated antibody in colloidal gold preparations and found some even in some "recently delivered" (they didn't say how long) samples. They recommended storing colloidal gold conjugates frozen, and testing them for free antibody before use by pelleting out the gold, labeling a sample with a dilution of the gold-free supernatent solution, then detecting the bound unconjugated IgG (in their case from goat) in the sample using a fluorochrome-labeled probe.
Gel filtration or size exclusion chromatography may be used to separate the unattached antibody from larger gold conjugates instead of pelleting and resuspending, and this also has the advantage of also removing aggregates (our colloidal gold probes are purified this way). I've also heard from a couple of sources that Jackson's 6 and 12 nm gold conjugates are very stable compared with other manufacturers.
Our 1.4 nm Nanogold=81 gold cluster conjugates are covalently cross-linked t= o the conjugate antibody rather than electrostatically adsorbed: this has been shown to give much improved stability. In our tests, these work identically after 12 months at 4=B0C, and have been used up to 2 years after preparation. These give superior cellular penetration and more quantitative labeling than equivalent colloidal gold conjugates (Vandr=E9 and Burry, J. Histochem. Cytochem, 1992 Vol. 40 p. 1837; Takizawa and Robinson, J. Histochem. Cytochem, 1994 Vol. 42 p. 1615). We cannot (yet) offer covalent conjugates with larger gold labels, but Nanogold=81 may be effectively silve= r enhanced.
Hope this is useful,
Rick Powell
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I'm having problems with LR White sections. Fixation of plant embryo material is fixed using 3% glutaraldehyde and buffer at 0 degrees overnight. Dehydration is from buffer through ethanol series to n-butanol. LR White and tissue are hardened by heating at 55 degrees C for 24 hours in a Beem capsule. Sectioning is on a Reichert:Jung 2040 using a carbide tungsten blade at 3 microns. Sections are placed on a drop of water before air drying. Staining consists of 5 min in 1% Acid Fushin, rinse, 5 min in 0.05% toluidine blue O in benzoate buffer, final rinsing, air drying and the addition of CytoSeal 60 mountant and a cover slip.
Problems start at the staining. Many sections float away, although I thought plastic sections "stuck". The end product has "ripples", but the section itself is not uneven. The staining looks great, but I can't photograph wavy sections. The sections do not seem to have "ripples" before staining, however the waviness may not be severe enough to notice before staining.
The protocol I'm trying to use was designed for GMA, not LR White, but I'd hoped to just substitute the less toxic choice.
Any help is greatly appreciated. Send responses directly to goodwib-at-wdni.com.
NOTE: This is a re-posting - the closing date has been changed by higher authorities to 3 January 1997...
RESEARCH ASSISTANT/RESEARCH FELLOW LEVEL A
We require an experienced person to set up and manage a new multi-institution confocal facility at Monash University in Melbourne, to be shared with groups from the Victoria University of Technology and CSIRO, located in the Department of Ecology and Evolutionary Biology. The facility will include both upright and inverted microscopes plus a stand-alone image analysis workstation.
Experience in use and maintenance of confocal microscope hardware and software is essential. Experience in a broad range of confocal applications in biology is highly desirable, so that the appointee could assist users in selecting the best technique for their particular project, and bring new applications to their notice on a regular basis. Familiarity with 3D reconstruction and image analysis applications is also desirable.
The position is available for one year in the first instance, with anticipated starting date of 1 March, 1997. Salary within the range $30,130 to $40,889 ($38,092 minimum with PhD) depending on experience. During 1997, users will require training in use of the microscope, and management protocols will be established. The appointee will be part of a small management committee representing the major user groups.
For further details, contact Dr. David Smyth, Department of Genetics and Developmental Biology, email David.Smyth-at-sci.monash.edu.au, ph. 61-3-9905-3861, fax 61-3-9905-5537 or Dr. Rosemary White, email r.g.white-at-sci.monash.edu.au.
Please send applications, including CV and names of three referees, to Ms. Annabel Carle, Department of Ecology and Evolutionary Biology, Monash University, Clayton, Victoria 3168, Australia.
Rosemary White Department of Ecology and Evolutionary Biology Monash University, Clayton, Victoria 3168, Australia phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au
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At 14:34 5/12/96 -0800, you wrote: } I'm having problems with LR White sections. Fixation of plant embryo } material is fixed using 3% glutaraldehyde and buffer at 0 degrees } overnight. Dehydration is from buffer through ethanol series to } n-butanol. LR White and tissue are hardened by heating at 55 degrees C } for 24 hours in a Beem capsule. Sectioning is on a Reichert:Jung 2040 } using a carbide tungsten blade at 3 microns. Sections are placed on a } drop of water before air drying. Staining consists of 5 min in 1% Acid } Fushin, rinse, 5 min in 0.05% toluidine blue O in benzoate buffer, final } rinsing, air drying and the addition of CytoSeal 60 mountant and a cover } slip. } } Problems start at the staining. Many sections float away, although I } thought plastic sections "stuck". The end product has "ripples", but } the section itself is not uneven. The staining looks great, but I can't } photograph wavy sections. The sections do not seem to have "ripples" } before staining, however the waviness may not be severe enough to notice } before staining. } } The protocol I'm trying to use was designed for GMA, not LR White, but } I'd hoped to just substitute the less toxic choice. } } Any help is greatly appreciated. Send responses directly to } goodwib-at-wdni.com. ******************************************* Dear goodwib et al - Try this: Plastic sections stick much better if they are heated. If heated in a drop of water they are likely to stretch better too. This may be the answer to those wrinkles too, but some very thick plant cell walls can be difficult in that respect. Within reason more heat causes the sections to stick better. Try 80 degrees for at least a couple of minutes after the water has evaporated. Use a single drop of the stains and wash off gently using squeeze bottle or a Pasteur pipette. Wash into a small dish, then, if needed you could retrieve the section and place it back onto a slide using a wire loop. For a big section use a wedge shaped piece of coverslip. Drawing a circle on the underside of the slide makes locating small sections easier. Hope this helps others with these common problems too. Cheers Jim Darley Probing & Structure (Microscopy Supplies & Accessories) PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313 A great microscopy site: http://www.ultra.net.au/~pns/
"Hi there! "Can anyone recommend a commercial source of good quality protein A "gold (20 nm). "Thanks "Dorota
I buy all my conjugate antibodies by Aurion ( sold by EMS in the USA) and I am really satisfied with. Aurion is a small compagny managed by Dr. Jan Leunissen. Many of you may know him through the books that he has written on Immunogold, and the courses that he teaches on the subject.Aurion Immunogold reagents have a guaranteed shelf life of 18 months from the date of the quality control analysis.
Jan Leunissen, Ph.D. AURION ImmunoGold Reagents & Accessories Costerweg 5, 6702 AA Wageningen The Netherlands
We have two electron microscopes for sale: 1) a Philips 400T TEM/STEM, with a total operation time less than 200 hours; 2) a Dedicated VG HB-501A STEM, which is about 9 years old and has all possible attachments on it.
Nan Yao Princeton Materials Institute Princeton University Bowen Hall, 70 Prospect Ave. Princeton, New Jersey 08540-5211
Hello, If drying down the section on a warming tray doesn't do it, we sometimes use charged slides to help them stick quicker. But the ripples may be from incomplete polymerization. If LR white's polymerization is interrupted by fluctuation in temperature or introduction of oxygen it may not restart. We always polymerize in a nitrogen atmosphere using an old paraffin vacuum oven hooked up to dry nitrogen.
Bob Morphology Core Univ. of Washington
On Thu, 5 Dec 1996, Brad Goodwin wrote:
} I'm having problems with LR White sections. Fixation of plant embryo } material is fixed using 3% glutaraldehyde and buffer at 0 degrees } overnight. Dehydration is from buffer through ethanol series to } n-butanol. LR White and tissue are hardened by heating at 55 degrees C } for 24 hours in a Beem capsule. Sectioning is on a Reichert:Jung 2040 } using a carbide tungsten blade at 3 microns. Sections are placed on a } drop of water before air drying. Staining consists of 5 min in 1% Acid } Fushin, rinse, 5 min in 0.05% toluidine blue O in benzoate buffer, final } rinsing, air drying and the addition of CytoSeal 60 mountant and a cover } slip. } } Problems start at the staining. Many sections float away, although I } thought plastic sections "stuck". The end product has "ripples", but } the section itself is not uneven. The staining looks great, but I can't } photograph wavy sections. The sections do not seem to have "ripples" } before staining, however the waviness may not be severe enough to notice } before staining. } } The protocol I'm trying to use was designed for GMA, not LR White, but } I'd hoped to just substitute the less toxic choice. } } Any help is greatly appreciated. Send responses directly to } goodwib-at-wdni.com. }
I have been asked to post this announcement. Please do not reply to the list.
Model: JEOL EM-100SX, with water chiller Installed: 11/86 On contract with JEOL until '89 Inspected by JEOL 4/96, fully functional at 100kV, but could use some repairs Location: Pacific northwest, USA Contact: Peg Baskerville, 1-800-557-0401
There has been much talk about "shelf life" of colloidal gold probes but little explanation of how this is evaluated.
We use protein A, and other protein-gold congujates for many different applications. For us, shelf life is determined by the final labeling efficiency i.e. is the signal the same as when we first made/purchased the probe?
As far as I know there are no published stereological studies on the labeling efficiency of gold probes over time. However, from experience I know that if we try to label biotinylated cells with streptavidin-gold that is more than 5 days old, we get no signal.
(Commercial produces of this probe need not worry because most applications involve detection of biotinylated antibodies followed by silver intensification, both producing colossal amplifications of the signal.)
In contrast, although I have been told on numerous occasions that protein A-gold has a shelf life of between 3 months to a year, I have used probes that are more than 6 years old and seen qualitative results similar to those produced using fresh probes. Unfortunately, other protein A-gold preparations have not aged so well.
For us there seem to have been little difference between storing the probes frozen in glycerol or in a 'fridge in the presence of azide. However (more dogma) I have been advised that freezing in glycerol will produce small aggregates of 2-3 particles. Again this is not something we have noticed.
From what is published it seems fair to conclude that the stability of a particular probe depends on the protein used to stabilize the colloid and on the amount of protein used. If these variables can be compared and correlated with labeling efficiency then it is worth sharing this data.
Posting opinions only serves to add further support to dogma.
Best regards,
Paul Webster, Ph.D Center for Cell Imaging Yale School of Medicine http://info.med.yale.edu/cellimg
I have been asked if there is a technique (optical microscopy, SEM, EDX, confocal (MRC1000 K/Ar laser) to determine whether calcium carbonate particulate is adhering to the surface of mesothelial cells (on the surface of a tissue sample). I suggested fixed tissue but CPD would probably result in loss of at least some of the adherent particulate. I could use cryoSEM of fixed tissue but I can't do EDX of frozen samples on our microscope. Is there a method I could use on the confocal? Questions that the researcher could not answer are: 1) is there particulate known to be present (don't know); 2) what size particulate (could be in the 10s of nanometers to hundreds of microns; well that covers just about everything!); 3) can there be intracellular deposits of calcium (yes, that's possible). I know that this is not very much to go on but it's all I can bring to the listserve. Hope someone can help and thanks in advance (TIA)
Damian Neuberger, Ph.D. Research Scientist Baxter International WG3-2S, P.O.Box 490 Round Lake, IL 60073 neuberd-at-baxter.com 847.270.5888 FAX 847.270.5897
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Try VisilogPro for the price of 2,995.00$US. In addition we have a new Materials Analysis module for an extra 1,000.00$.
This module allows to rebuild grains using advanced morphology plus over a dozen other functions related to grain sizing and imaging in microscopy.
At 10:07 AM 12/6/96 CST, MCOV%mimi-at-magic.itg.ti.com wrote: } From: Mike Coviello MCOV } } Subj: EM software-need to locate } } } Can anyone recommend any inexpensive software (or shareware) for: 1) Indexing } of diffraction patterns 2) Grain-size analysis in PC or Mac format(please } specify which in your response). } } Michael Coviello } Central Research Laboratories } Texas Instruments } Dallas, TX } }
---------------------------------------------------------------------------- --------------------- Luc Nocente Tel: 514 345 1400 Noesis Vision Inc. Fax: 514 345 1575 e-mail: ln-at-noesisvision.com 6800 Cote de Liesse, Suite 200 St-Laurent, PQ H4T 2A7,Canada
Visit our new web site at http://www.noesisvision.com ---------------------------------------------------------------------------- ---------------------
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Hi Everybody, Does somebody has heard something about an enhancement for laser printers called LAZARPRINT and abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know it looks impossible) I'm interresting by technical information and manufacturer. Salutations. ========================================================== Jacky Larnould tel 33 (0)4 67 72 28 26 fax 33 (0)4 67 79 54 90 email larnould-at-mnet.fr
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G'day Subscribers....
Sorry, but I took the system down for a day for some system maintenance. Everything should now be back on-line. Hopefully no-body noticed the shutdown. If your postings over the weekend bounced then please resend them.
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} I have been asked if there is a technique (optical microscopy, SEM, } EDX, confocal (MRC1000 K/Ar laser) to determine whether calcium } carbonate particulate is adhering to the surface of mesothelial cells } (on the surface of a tissue sample). I suggested fixed tissue but CPD } would probably result in loss of at least some of the adherent } particulate. I could use cryoSEM of fixed tissue but I can't do EDX } of frozen samples on our microscope. Is there a method I could use on } the confocal? Questions that the researcher could not answer are: 1) } is there particulate known to be present (don't know); 2) what size } particulate (could be in the 10s of nanometers to hundreds of microns; } well that covers just about everything!); 3) can there be } intracellular deposits of calcium (yes, that's possible). I know that } this is not very much to go on but it's all I can bring to the } listserve. Hope someone can help and thanks in advance (TIA) } } Damian Neuberger, Ph.D.
Since the deposits survive fixation, you can dry the cells in hexamethyldisilizane, which is mechanically very gentle. Might give better morphology as well. After dehydration in EtOH, transfer to HMDS through a 2:1 EtOH:HMDS 1:2 series, then 3X100% HMDS using the same times as for 100% EtOH; dry at room temperature or 60 degree (or 45 degree) C oven (the different temperatures can give different results) Phil
&&& Illigitimi non carborundum &&&&&&&& Philip Oshel Microscopy Station A PO Box 5037 Champaign, IL 61825-5037 (217)244-3145 days (217)355-3145 evenings oshel-at-ux1.cso.uiuc.edu *** looking for a job again ******************
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Hi Everybody, Does somebody has heard something about an enhancement for laser printers called LAZARPRINT and abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know it looks impossible) I'm interresting by technical information and manufacturer. Salutations. ========================================================== Jacky Larnould tel 33 (0)4 67 72 28 26 fax 33 (0)4 67 79 54 90 email larnould-at-mnet.fr
It just occured to me, that in the new configuration of the Listserver a few of you may have minor problems. This warning only applies only to those users who employ filters on their Email messages to sort and trash unwanted messages.
You may notice that I have recently modified the server so that it prepends a few short descriptive lines to always *remind* people the correct address for adding and/or removing their Email addresses to the Listserver database.
I bring this up because some of you will be likely using Email programs (such as Eudora) which can filter messages based upon key words in the headers and/or body of the text. The key words which may cause you problems are:
S-bscribe ( the letter "u" omitted here) or Uns-bscribe (as above)
In the scenerio which I envision, a user will employ an Email filter based upon the above words to automatically trash Email messages which would likely be meaningless to them, but obviously important to the Listserver operation. (I have left out the "u's" above so that everyone will see this particuliar posting)
Since both these key words now appear in the body of *every* text message which is posted to the Listserver you will have to change your filters! I would suggest doing a double filter on messages and if you find both the words (i.e. S-bscribe/Uns-bscribe) in the message then do not automatically trash the message.
I realize that this may inconvenience a few of our subscribers but I'm trying to find a simple way to benefit the largest body of people and remind everyone routinely the correct address to S-bscribe and Uns-bscribe from the Server. In the long run I believe this will reduce the number of incorrect postings.
Just for the record the majority (} 50%) of S-bscribe and Uns-bscribe messages are correctly sent to ListServer-at-MSA.Microscopy.Com. I'm hoping in this iteration to bring that number up to the 90% level.
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At 18:50 07/12/1996 +0100, you wrote: } } Does somebody has heard something about an enhancement for laser printers } called LAZARPRINT and=20 } abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I= know } it looks impossible) } } Dear Microscopists,
Impossible or not, this product really exists !!!
In addition to the high quality grayscale images, the speed of the process is also remarkable: due to a direct access to the video port of the printer via a special interface, a picture of letter size is transferred and printed out within the time the laser printer needs for paper skip alone (+/- 10 seconds !).
The near photographic quality obtained at a very low cost (use of plain paper) makes the LazarPrint system the ideal solution if you want to print pictures obtained with really high resolution grabbing systems (eg our ORION 4.2 for Windows that gives you a resolution up to 4000 x 4000 pixels and 256 gray levels).
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D See our web site: http://www.microscopy-uk.org.uk =20
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Chere Jacky:
The little gizmoes which make a low DPI printet think it's a high DPI printer ARE A RIP OFF. They are fiddling the system and really don't give you any real additional information. Contact John Mackenzie at {supervisor-at-emc.ncsu.edu} who is the expert on these matters.
Patrick Echlin Cambridge UniversityOn Sat, 7 Dec 1996, LARNOULD J. wrote:
} Hi Everybody, } Does somebody has heard something about an enhancement for laser printers } called LAZARPRINT and } abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know } it looks impossible) } I'm interresting by technical information and manufacturer. } Salutations. } ========================================================== } Jacky Larnould } tel 33 (0)4 67 72 28 26 } fax 33 (0)4 67 79 54 90 } email larnould-at-mnet.fr } }
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At 18:26 08/12/96 +0100, you wrote: } } Hi Everybody, } Does somebody has heard something about an enhancement for laser printers } called LAZARPRINT and } abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know } it looks impossible) } I'm interresting by technical information and manufacturer. } Salutations. } ========================================================== } Jacky Larnould } } Society : LEUTRON VISION GmbH D-82216 Gernlinden (Germany) FAX : (0 81 42) 4 02 19
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At 06:50 PM 12/7/96 +0100, you wrote: } Hi Everybody, } Does somebody has heard something about an enhancement for laser printers } called LAZARPRINT and } abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know } it looks impossible) } I'm interresting by technical information and manufacturer. } Salutations. } ========================================================== } Jacky Larnould } tel 33 (0)4 67 72 28 26 } fax 33 (0)4 67 79 54 90 } email larnould-at-mnet.fr } } } We use the LazarPrint print board in a HP 4+ laser printer. The board allows the printer to print at 4800 dpi. We purchased ours from Smart Analytical Products in Maryland, USA. However, the board is made in Germany, and I am do know who markets the board in Europe. The board is very fast. You can even print out multiple copies of 20MB+ image files in the same manner you would print a text file. The normal output of the laserjet produces a dot pattern that is easily seen if you examine a print with a magnifying glass. If you look at the grain stucture of a picture printed using the LazarPrint board, the dots are almost too small too seen. Each pixel can accept 1024 gray levels, but only 256 of these will be printed. However, this allows a great deal of contrast manipulation. The board does an excellent job with electron micrographs. I have no commercial interest in the board.
Regards,
John J. Turek, Ph.D. Purdue University Dept. of Basic Medical Sciences Core Laboratory for Image Analysis and Multidimensional Applications (CRISTAL) phone: 317-494-5854 fax: 317-494-0781 email: jjt-at-vet.purdue.edu
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{fontfamily} {param} Times {/param} {bigger} {bigger} Patrick, understanding some of the principles of LaserJet printing may help to draw some more objective conclusions:
LaserJets like many other printers print in lines (lpi). The lines represent in digital image printing lines of pixels. If the pixels are printed squarely than each square area (of a width equal to that of the actual line width) must be printed with a desired amount of ink variations in order to generate the gray levels (see John Russ' book for a nice illustration on how this is done in a LaserJet). LaserJets and inkjets use dots as the smallest printed entity. If you would print with 600 dpi and 100 lpi (LaserJet 4) than you could place 6 dots per line (600/100=6), As a consequence you can maximally place 6x6 dots per pixel or make 36 gray levels plus no-dots (white). Thus a LaserJet 4M will print with 37 gray levels. This is just at the limit of gray level recognition. A 1200 dpi printer which uses 100 lpi, will be able to generate 145 gray levels per pixel (1200/100 = 12; 12x12+1=145). That's why the Lexmark is so good because the eye can't distinguish that amount of gray levels (beyond the contrast resolution of our eyes). However, 100 lpi (25:100= 0.25 mm) also is just at the recognition level of the eye with regards to spatial resolution. Print heads in LaserJets can print much better and ,if combined with HP's superfine ink powders, they can easily be driven at 4800 dpi on plain paper. There are many high-resolution printers on the market which do better. However, for comparison, print speed is as important as adequate print resolution. LazarPrint prints 300 lines per inch (lpi) and 256 gray levels, both surely beyond the resolution limit of the unaided eye and thus producing "photography like" printing quality. However, in addition, LazarPrint prints 1- 10 MB of images per page in 20 sec. (on a conventional LaserJet).
If you like to see some proof, look at a comparison of these printers at the following web site, where dpi/lpi/eye resolution are compared for a 1Kx1Kx8-bit test image:
Patrick, trust physics and experimental proof not emotions. I don't like gizmos either, that's why I took the time and compared the actual print quality. Than, I made up my mind and for now over two years I never used my photolab again, but I printed over 20,000 images (pages with up to 8 images each) on my LaserJet, submitted MSA and other abstracts, made all my slides and published papers; all with the help of my old but good LaserJet.
Address of LazarPrint developer: bm484646-at-muenchen.org (Mikel Jaeth)
I am commercially not involved in any of the mentioned resources. Best season greetings Klaus
****************
} Chere Jacky:
}
} The little gizmoes which make a low DPI printet think it's a high DPI
} printer ARE A RIP OFF. They are fiddling the system and really don't
} give you any real additional information. Contact John Mackenzie at
} { {supervisor-at-emc.ncsu.edu} who is the expert on these matters.
}
} Patrick Echlin
} Cambridge UniversityOn Sat, 7 Dec
} 1996, LARNOULD J. wrote:
}
} } Hi Everybody,
} } Does somebody has heard something about an enhancement for laser printers
} } called LAZARPRINT and
} } abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
} } it looks impossible)
} } I'm interresting by technical information and manufacturer.
converted ((1) (0) (10021) (7) (1) (0) (1), (1) (0) (10021) (7) (1) (0) (6)); Relayed; 9 Dec 1996 17:18:07 +0100 X400-Received: by mta area3.rueilmta in /PRMD=ifp/ADMD=atlas/C=fr/; converted ((1) (0) (10021) (7) (1) (0) (1), (1) (0) (10021) (7) (1) (0) (6)); Relayed; 9 Dec 1996 16:21:38 +0000
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Dear all,
I have allready sent this message last week, but I am not sur I did it right... So I am going to bother you once more... Sorry for those who have already received this request.
I am trying to get some information on any kind of relevant tests that can be done to evaluate TEM performances. I want to be sur I gonna use the right method and I gonna do it right !!
Does any body know by heart articles or books giving (practical and theoritical) details on specific methods to test resolution (calculation of Cs..), information limit (like ODM + Young Franges...), stability, drift, analytical performances ??..
If you have any good advises on evaluating a TEM before buying it, I will appreciate...
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In resonse to the most recent discussion on magnetic fields, I thought I might be able to add some insight from the experiences we have had minimizing them in our facility. As a little background, our facility was built about 5 years ago and was "designed" specifically to house our electron microscopes. From the beginning we had magnetic field problems in some of our microscope rooms (but not others), with field strengths as high as 3-4 mG at a couple of the microscopes. In the service corridor that runs behind the microscope rooms (power supplies, water chillers, elecrtrical breaker boxes, etc. are located there) field strengths greater than 20 mG were measured near some of the breaker boxes. These fields did not go away even if all of the breakers were tripped. They may have decreased somewhat but they certainly were not reduced to acceptable levels. In fact, tripping the switches at the main distribution panel to cut off the power to our entire facility simultaneously did not cause the fields to go away. We muddled around with several attempted fixes such as having electricians replace sections of electrical conduit with plastic tubing and putting rubber insulators around the conduit where it was mounted into the walls or ceilings. We had various degrees of success with these "fixes" and some may have made things worse. After meeting directly with an outside consultant we were able to get a good understanding of what was necessary to eliminate the fields. Our problems were entirely due to there being paths to ground other than the ground wires themselves so that the ground did not run back to the electrical box. When this happens there is a net electrical current on the conduit and wires. It is this net current that gives rise to the electromagnetic fields. If I remember correctly the magnetic field associated with this current drops off as a function of 1/r. This is compared to the fields originating from electronic equipment that drop off as a function of 1/(r^3). I think you will also see this 1/r cubed response if you measure the field from a power cord that has no net current on it. In my opinion the most important tool for tracking down the sources of the fields is an AC current probe. A number of different models are available that plug into a digital multimeter. Ours cost about $120 from Grainger. It works by simply clamping it around the wire or conduit you want to check. If the current readout is not zero you have a ground path somewhere that needs to be eliminated. A magnetic field meter is also useful for general monitoring of field strengths and tracking down sources behind walls and other inaccessible places. Our meter is a Holaday Industries HI-3624A that we paid under $500 for from Holaday Industries, Eden Prairie, MN ph.612-934-4920 (no financial interest, just a satisfied user of a product from a local company). In the end we were able to eliminate almost all of our electromagnetic field sources and the readings at the scopes are down to about 0.2 mG. If I were planning a new facility I would insist that isolated ground recepticles and switches be used everywhere. In the typical recepticle there is a place to attach a ground wire but it is not electrically isolated from the mounting yoke of the recepticle. So, in our case where the recepticles were mounted into a metal wire mold that in turn was screwed into the metal studs used for framing the rooms we ended up with all kinds of possible ground paths. In this instance we were able to isolate the wire mold from the framing using nylon washers around all of the mounting screws, but could have had to replace all of our recepticles (at much greater expense). The wiring to the light fixtures was also a major field source. In this instance, our fixtures themselves could not be isolated so the entire conduit run back to the electrical box had to be isolated from possible alternative ground paths. This is where the current probe was most useful. By checking the conduit on both sides of the possible grounding points it could be quickly determined if isolation was necessary, and if so rubber bushings could be installed. In regard to the observance of fields when power was "disconnected," tripping the switch simply breaks the hot wire and a current can still flow along the neutral wire to the unintended grounding points. When we first observed this we thought the field source may have been external to our electrical system and would require active EM field cancellation systems ($$$). We do still have a number of very of minor field sources, but they haven't been worth the effort to eliminate. For all you who have also had to deal with EM fields I hope this was of a little help. Mike
Mike Bench Characterization Facility Center for Interfacial Engineering University of Minnesota Voice: (612) 624-6590 Fax: (612) 626-7530 e-mail: bench-at-cems.umn.edu
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Forwarded to: smtp-at-eye-at-servers[microscopy-at-sparc5.microscopy.com] cc: Comments by: Yuhui Xu-at-RES-at-DFCI
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The original message was received at Mon, 9 Dec 1996 14:20:59 -0500 (EST) from eye.dfci.harvard.edu [134.174.76.49]
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We have an MT2 (working) and an MT2B (needs new o-rings installed, there are some which are available with the microtome) Sorvall Microtomes which are available for sale or to the highest bidder. If you have any questions, or would like more information, You can call, fax or email me at the numbers listed below. 12/9/96
Mei Lie Wong Department of Biochemistry HHMI-UCSF Ph. 415-476-4441 Fax 415-476-1902 email wong-at-msg.ucsf.edu
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I like to save your message in my computer but I have difficulties. I am using WordPerfect 7; it does not recognize what format the text is. Can you kindly resend it with Ascii or a wordperfect formats? Thank you kindly.
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I sent you a message just few minutes ago asking you to resend your message. I misquoted the version of my WordPerfect. It is a WP 6.1 not 7. Sorry.
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} I am trying to get some information on any kind of relevant tests that can be } done to evaluate TEM performances. I want to be sur I gonna use the right method } and I gonna do it right !! } } Does any body know by heart articles or books giving (practical and theoritical) } details on specific methods to test resolution
The simplest method is to image a gold film, find suitably fine spa- cings, and see what is the finest which can be resolved. Another method is to take a through-focus series of a holey amorphous carbon film and measure the interference fringe width at focus. The latter is a very good test of overall performance.
(calculation of Cs..),
O. Krivanek has a procedure using amorphous C or Ge. Take images at several values of defocus. The fringe spacings are related to Cs, and one can use least-squares to fit the spacings at various defocus values to Cs. There is an article on this in Ultramicroscopy (I do not have it in front of me, but I could look up the volume & pages if you wish). Good luck. Yours, Bill Tivol
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{fontfamily} {param} Times {/param} {bigger} {bigger} Patrick, understanding some of the principles of LaserJet printing may help to draw some more objective conclusions:
LaserJets like many other printers print in lines (lpi). The lines represent in digital image printing lines of pixels. If the pixels are printed squarely than each square area (of a width equal to that of the actual line width) must be printed with a desired amount of ink variations in order to generate the gray levels (see John Russ' book for a nice illustration on how this is done in a LaserJet). LaserJets and inkjets use dots as the smallest printed entity. If you would print with 600 dpi and 100 lpi (LaserJet 4) than you could place 6 dots per line (600/100=6), As a consequence you can maximally place 6x6 dots per pixel or make 36 gray levels plus no-dots (white). Thus a LaserJet 4M will print with 37 gray levels. This is just at the limit of gray level recognition. A 1200 dpi printer which uses 100 lpi, will be able to generate 145 gray levels per pixel (1200/100 = 12; 12x12+1=145). That's why the Lexmark is so good because the eye can't distinguish that amount of gray levels (beyond the contrast resolution of our eyes). However, 100 lpi (25:100= 0.25 mm) also is just at the recognition level of the eye with regards to spatial resolution. Print heads in LaserJets can print much better and ,if combined with HP's superfine ink powders, they can easily be driven at 4800 dpi on plain paper. There are many high-resolution printers on the market which do better. However, for comparison, print speed is as important as adequate print resolution. LazarPrint prints 300 lines per inch (lpi) and 256 gray levels, both surely beyond the resolution limit of the unaided eye and thus producing "photography like" printing quality. However, in addition, LazarPrint prints 1- 10 MB of images per page in 20 sec. (on a conventional LaserJet).
If you like to see some proof, look at a comparison of these printers at the following web site, where dpi/lpi/eye resolution are compared for a 1Kx1Kx8-bit test image:
Patrick, trust physics and experimental proof not emotions. I don't like gizmos either, that's why I took the time and compared the actual print quality. Than, I made up my mind and for now over two years I never used my photolab again, but I printed over 20,000 images (pages with up to 8 images each) on my LaserJet, submitted MSA and other abstracts, made all my slides and published papers; all with the help of my old but good LaserJet.
Address of LazarPrint developer: bm484646-at-muenchen.org (Mikel Jaeth)
I am commercially not involved in any of the mentioned resources. Best season greetings Klaus
****************
} Chere Jacky:
}
} The little gizmoes which make a low DPI printet think it's a high DPI
} printer ARE A RIP OFF. They are fiddling the system and really don't
} give you any real additional information. Contact John Mackenzie at
} { {supervisor-at-emc.ncsu.edu} who is the expert on these matters.
}
} Patrick Echlin
} Cambridge UniversityOn Sat, 7 Dec
} 1996, LARNOULD J. wrote:
}
} } Hi Everybody,
} } Does somebody has heard something about an enhancement for laser printers
} } called LAZARPRINT and
} } abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
} } it looks impossible)
} } I'm interresting by technical information and manufacturer.
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Dear Klaus: Thank you for your patient explanation of Laser Jet printing. We tried these gizmo's about two years ago and at that time could see no improvement. We now use a 600dpi pinter of a clay covered brilliant white paper using superfine print powder and are satisfied with the results. Perhaps things have changed in the past two years.
Sincereley
Patrick
On Mon, 9 Dec 1996, Klaus-Ruediger Peters wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer is Sponsored by: } the Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } ------------------------------------------------------------------------------ } |WWW| http://WWW.MSA.Microscopy.Com |Email| BusinessOffice-at-MSA.Microscopy.Com } ------------------------------------------------------------------------------. } } {fontfamily} {param} Times {/param} {bigger} {bigger} Patrick, understanding } some of the principles of LaserJet printing may help to draw some more } objective conclusions: } } } LaserJets like many other printers print in lines (lpi). The lines } represent in digital image printing lines of pixels. If the pixels are } printed squarely than each square area (of a width equal to that of } the actual line width) must be printed with a desired amount of ink } variations in order to generate the gray levels (see John Russ' book } for a nice illustration on how this is done in a LaserJet). LaserJets } and inkjets use dots as the smallest printed entity. If you would print } with 600 dpi and 100 lpi (LaserJet 4) than you could place 6 dots per } line (600/100=6), As a consequence you can maximally place 6x6 dots per } pixel or make 36 gray levels plus no-dots (white). Thus a LaserJet 4M } will print with 37 gray levels. This is just at the limit of gray level } recognition. A 1200 dpi printer which uses 100 lpi, will be able to } generate 145 gray levels per pixel (1200/100 = 12; 12x12+1=145). That's } why the Lexmark is so good because the eye can't distinguish that } amount of gray levels (beyond the contrast resolution of our eyes). } However, 100 lpi (25:100= 0.25 mm) also is just at the recognition } level of the eye with regards to spatial resolution. Print heads in } LaserJets can print much better and ,if combined with HP's superfine } ink powders, they can easily be driven at 4800 dpi on plain paper. } There are many high-resolution printers on the market which do better. } However, for comparison, print speed is as important as adequate print } resolution. LazarPrint prints 300 lines per inch (lpi) and 256 gray } levels, both surely beyond the resolution limit of the unaided eye and } thus producing "photography like" printing quality. However, in } addition, LazarPrint prints 1- 10 MB of images per page in 20 sec. (on } a conventional LaserJet). } } } If you like to see some proof, look at a comparison of these printers } at the following web site, where dpi/lpi/eye resolution are compared } for a 1Kx1Kx8-bit test image: } } } http://panda.uchc.edu/htklaus/DigiLab/Printing-ResultsL.html } } } Patrick, trust physics and experimental proof not emotions. I don't } like gizmos either, that's why I took the time and compared the actual } print quality. Than, I made up my mind and for now over two years I } never used my photolab again, but I printed over 20,000 images (pages } with up to 8 images each) on my LaserJet, submitted MSA and other } abstracts, made all my slides and published papers; all with the help } of my old but good LaserJet. } } } Address of LazarPrint developer: bm484646-at-muenchen.org (Mikel Jaeth) } } } I am commercially not involved in any of the mentioned resources. Best } season greetings Klaus } } } **************** } } } Chere Jacky: } } } } } } The little gizmoes which make a low DPI printet think it's a high DPI } } } printer ARE A RIP OFF. They are fiddling the system and really don't } } } give you any real additional information. Contact John Mackenzie at } } } { {supervisor-at-emc.ncsu.edu} who is the expert on these matters. } } } } } } Patrick Echlin } } } Cambridge UniversityOn Sat, 7 Dec } } } 1996, LARNOULD J. wrote: } } } } } } } Hi Everybody, } } } } Does somebody has heard something about an enhancement for laser } printers } } } } called LAZARPRINT and } } } } abble to increase the resolution at 6800 DPI and 1024 Gray } levels!!!(I know } } } } it looks impossible) } } } } I'm interresting by technical information and manufacturer. } } ************* } } } } {/bigger} {/bigger} {/fontfamily} } } xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx } } x Bald ist es wieder so weit (It will be happening soon) } x x } x } } x * { {|:-)= Santa Claus... } x } } x } x } } xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx } } } }
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Dear colleagues, Dear friends, We (Ali Temara and Michel Warnau) became new young fathers in the course of November 1996. We'd like first to announce it to the world as we are incredibly proud of our sons (Rayan and Nathan, respectively). Then we would like to ask to all of our colleagues and friends around the world to try to find the newspaper that was published on the day of their birth (November 17th and November 27th). Indeed, it is likely that you and/or a member of your family or a colleague keep newspapers without any particular aim and throw them away once a month. We would especially appreciate the front page of your regional newspapers. We take the opportunity of this message to wish you a Merry Christmas and a happy and prosperous New Year! Kindest regards,
Ali & Michel
________________________________________________________________ Dr. Ali Temara and Dr. Michel Warnau
Laboratoire de Biologie Marine (CP 160/15) av. F.D. Roosevelt 50 B-1050 Brussels (BELGIUM)
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Hello fellow microscopists, Does anyone have experience in running an Iomega ZIP drive under Novell 7 DOS on a network? My computer Pentium 100, Windows 3.1 hangs when running the guest driver; if I boot on MS DOS 6.2 the drive runs fine. Have not been able to get help from Iomega on this.
Thank you, and Season's Greetings Prof.Walter A.Mannheimer Metallurgy and Materials Engineering Federal University of Rio de Janeiro POBox 68505 21945 Rio de Janeiro, Brazil Voice +5521 280-7443 (Dept.office) +5521 590-0579 (direct) Fax +5521 290-6626 Email: wamann-at-metalmat.ufrj.br
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While I appreciate the pride you have in the birth of your family, this is an inappropriate use of the Microscopy Listserver. DO NOT do this again!!!!!
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} Sir, } } You might get some help from Iomega's support site: } } http://www.iomega.com/support/techs/zip/1.html#unix } } } ------------------------------------------------- } Harold J. Crossman } OSRAM SYLVANIA INC. } Lighting Research Center } 71 Cherry Hill Dr. } Beverly, MA 01915 } Phone: (508) 750-1717 } E-mail: crossman-at-rd.sylvania.com } } Our web sites: www.sylvania.com } www.siemens.com } -- } } "Crossman, Harold" {crossman-at-RD.SYLVANIA.com
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It's not interesting for me. Please be polite and never post such messages.
On Tue, 10 Dec 1996, Michel Warnau wrote:
} ------------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsored by The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } ------------------------------------------------------------------------------. } } Dear colleagues, Dear friends, } We (Ali Temara and Michel Warnau) became new young fathers in the } course of November 1996. We'd like first to announce it to the world as } we are incredibly proud of our sons (Rayan and Nathan, respectively). } Then we would like to ask to all of our colleagues and friends around } the world to try to find the newspaper that was published on the day of } their birth (November 17th and November 27th). Indeed, it is likely that } you and/or a member of your family or a colleague keep newspapers without } any particular aim and throw them away once a month. We would especially } appreciate the front page of your regional newspapers. } We take the opportunity of this message to wish you a Merry Christmas } and a happy and prosperous New Year! } Kindest regards, } } Ali & Michel } } ________________________________________________________________ } Dr. Ali Temara and Dr. Michel Warnau } } Laboratoire de Biologie Marine (CP 160/15) } av. F.D. Roosevelt 50 } B-1050 Brussels (BELGIUM) } } Phone: 32/2/650 29 70 - 32/2/650 22 34 Fax: 32/2/650 27 96 } e-mail: atemara-at-ulb.ac.be } mwarnau-at-ulb.ac.be }
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Forwarded message:
I have a Macintosh Quadra 840AV which seems quite suitable for recording and editing video movies as it comes with an S-video input port. What I'm interested in is recording fairly slow processes such as cell division in microscopic algae using a video camera attached to a microscope, capturing an image, say every minute or so. Has anyone used an 840AV (or any other Mac) for this purpose and what additional software (apart from the built-in Video Monitor and FusionRecorder software) would be needed, if any, to do the job properly?
Hans Sluiman Royal Botanic Garden Edinburgh h.sluiman-at-rbge.org.uk
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RE} } EM fields 12/10/96
Very informative, thank-you for the info. I have some serious field problems around a 6300FE JEOL. I've sent copies of your reply to the facilities people here. All attempts to locate the problem thus far have been futile. Your suggestions are new to us and we will try them.
********************************************************** Jake Schaper Product Analysis Lab Advanced Digital Consumer Division Motorola, Inc. Chandler, Az. **********************************************************
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In resonse to the most recent discussion on magnetic fields, I thought I might be able to add some insight from the experiences we have had minimizing them in our facility. As a little background, our facility was built about 5 years ago
and was "designed" specifically to house our electron microscopes. From the beginning we had magnetic field problems in some of our microscope rooms (but not others), with field strengths as high as 3-4 mG at a couple of the microscopes. In the service corridor that runs behind the microscope rooms (power supplies, water chillers, elecrtrical breaker boxes, etc. are located there) field strengths greater than 20 mG were measured near some of the breaker boxes. These fields did not go away even if all of the breakers were tripped. They may have decreased somewhat but they certainly were not reduced to acceptable levels. In fact, tripping the switches at the main distribution panel to cut off the power to our entire facility simultaneously did not cause the fields to go away. We muddled around with several attempted fixes such as having electricians replace sections of electrical conduit with plastic tubing and putting rubber insulators around the conduit where it was mounted into the walls or ceilings. We had various degrees of success with these "fixes" and some may
have made things worse. After meeting directly with an outside consultant we were able to get a good understanding of what was necessary to eliminate the fields. Our problems were
entirely due to there being paths to ground other than the ground wires themselves so that the ground did not run back to the electrical box. When this happens there is a net electrical current on the conduit and wires. It is this
net current that gives rise to the electromagnetic fields. If I remember correctly the magnetic field associated with this current drops off as a function of 1/r. This is compared to the fields originating from electronic equipment that drop off as a function of 1/(r^3). I think you will also see this 1/r cubed response if you measure the field from a power cord that has no net current on it. In my opinion the most important tool for tracking down the sources of the fields is an AC current probe. A number of different models are available that
plug into a digital multimeter. Ours cost about $120 from Grainger. It works by simply clamping it around the wire or conduit you want to check. If the current readout is not zero you have a ground path somewhere that needs to be eliminated. A magnetic field meter is also useful for general monitoring of field strengths and tracking down sources behind walls and other inaccessible places. Our meter is a Holaday Industries HI-3624A that we paid under $500 for
from Holaday Industries, Eden Prairie, MN ph.612-934-4920 (no financial interest, just a satisfied user of a product from a local company). In the end we were able to eliminate almost all of our electromagnetic field sources and the readings at the scopes are down to about 0.2 mG. If I were planning a new facility I would insist that isolated ground recepticles and switches be used everywhere. In the typical recepticle there is a place to attach a ground wire but it is not electrically isolated from the mounting yoke of the recepticle. So, in our case where the recepticles were mounted into a metal wire mold that in turn was screwed into the metal studs used for framing
the rooms we ended up with all kinds of possible ground paths. In this instance we were able to isolate the wire mold from the framing using nylon washers around all of the mounting screws, but could have had to replace all of our recepticles (at much greater expense). The wiring to the light fixtures was also a major field source. In this instance, our fixtures themselves could not be isolated so the entire conduit run back to the electrical box had to be isolated from possible alternative ground paths. This is where the current probe was most useful. By checking the conduit on both sides of the possible grounding points
it could be quickly determined if isolation was necessary, and if so rubber bushings could be installed. In regard to the observance of fields when power was "disconnected," tripping the switch simply breaks the hot wire and a current can still flow along the neutral wire to the unintended grounding points. When
we first observed this we thought the field source may have been external to our electrical system and would require active EM field cancellation systems ($$$). We do still have a number of very of minor field sources, but they haven't been worth the effort to eliminate. For all you who have also had to deal with EM fields I hope this was of a little help. Mike
Mike Bench Characterization Facility Center for Interfacial Engineering University of Minnesota Voice: (612) 624-6590 Fax: (612) 626-7530 e-mail: bench-at-cems.umn.edu
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Ali & Michel;
While we all appreciate your excitement regarding the arrival of your new family members, perhaps we should remember the goal of this listserver, which is to disseminate information on microscopy-related topics. Thanks for your consideration.
(BTW, congratulations)
******************************************* Bob Citron Chiron Vision 555 W. Arrow Hwy Claremont, CA 91711 USA email: Bob_Citron-at-cc.chiron.com *******************************************
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Dear colleagues, Dear friends, We (Ali Temara and Michel Warnau) became new young fathers in the course of November 1996. We'd like first to announce it to the world as we are incredibly proud of our sons (Rayan and Nathan, respectively). Then we would like to ask to all of our colleagues and friends around the world to try to find the newspaper that was published on the day of their birth (November 17th and November 27th). Indeed, it is likely that you and/or a member of your family or a colleague keep newspapers without any particular aim and throw them away once a month. We would especially appreciate the front page of your regional newspapers. We take the opportunity of this message to wish you a Merry Christmas and a happy and prosperous New Year! Kindest regards,
Ali & Michel
________________________________________________________________ Dr. Ali Temara and Dr. Michel Warnau
Laboratoire de Biologie Marine (CP 160/15) av. F.D. Roosevelt 50 B-1050 Brussels (BELGIUM)
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Hello,
I looking for information about carbon TEM grids. I would like to find out the following:
typical mesh size fabrication process manufacturer applications
Anything you can tell me, or any reference, would be quite useful. = Thanks in advance. Please respond to the following email address: oschueller-at-gmwgroup.harvard.edu
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Professor Mannheimer wrote: } Hello fellow microscopists, } Does anyone have experience in running an Iomega ZIP drive under } Novell 7 DOS on a network? } My computer Pentium 100, Windows 3.1 hangs when running the guest } driver; } if I boot on MS DOS 6.2 the drive runs fine. } Have not been able to get help from Iomega on this.
We at MOXTEK have been enthusiastic about Novell Dos for a long time, so I asked around. They say that Zip drives don't work with Novel Dos 7 because the emm386 is not working right. If you REM out the emm386 load in config.sys it should then work fine.
best regards mark
Mark W. Lund, PhD Director } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. ************************************************* Orem UT 84057 **"Soft x-rays in the 21st Century" conference ** 801-225-0930 ** 8-11 January 1997, Midway Utah ** FAX 801-221-1121 ** http://volta.byu.edu/xray/info.html ** lundm-at-xray.byu.edu *************************************************
"Let me commend a great truth to you which has been one of the supports of my life: 'The Gods send threads for a web begun.' Andrew Carnegie
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Hello,
I have an environmental engineer who is interested at looking at bacterial samples that have been filtered. He wants to look at the bacteria on the filters themselves. Does anyone know how to process such samples? Do I let the filters air-dry or should I fix, dehydrate, and CPD the filters? Any help would be much appreciated.
Thank you in advance,
Ginger Baker EM Lab Manager Dept. APP 250 Veterinary Medicine Oklahoma State University Stillwater, OK 74078 (405) 744-6765 FAX: (405) 744-5275 Email: lizard-at-okway.okstate.edu
One of our customers has had a rather strong allergic reaction during the handling and use of two embedding media simultaneously. One of the products was Unicryl and the other, Technovet. I am very concerned about the prevalence of such allergic reactions with acrylates, and am presently attempting to gather all pertinent literature and/or subscriber experiences with acrylate allergies.
A literature search on MEDLINE has revealed only one paper describing contact dermatitis reaction to Lowicryl, but many papers concerning the allergies to methacrylates used as adhesives by the dental profession and for cosmetic purposes.
I would appreciate learning of any experiences of the members and to receive any pertinent references regarding allergies to acrylate embedding media.
Regards, Don Cox ******************************************************** Donald P. Cox, Ph.D., M.B.A. GOLDMARK BIOLOGICALS/D.P. COX ASSOCIATES 437 Lock Street, Phillipsburg, NJ 08865-2764 (908) 859-2631 - - (908) 859-2875-FAX E-Mail: goldmrkr-at-fast.net/goldmarker-at-aol.com Web Page: http://members.aol.com/goldmarker
~~~"Goldmarking is everlasting probing!"~~~ ********************************************************
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} Dear Colleagues: {SNIP} } One of our customers has had a rather strong allergic reaction during the } handling and use of two embedding media simultaneously. One of the products } was Unicryl and the other, Technovet. I am very concerned about the } prevalence of such allergic reactions with acrylates, and am presently } attempting to gather all pertinent literature and/or subscriber experiences } with acrylate allergies. {SNIP}
Hi! Aside from the allergies - is it possible to request for the address of the supplier of technovit/technovet. I have heard that technovit resin gives excellent embedding for plant tissues, and I am wondering if anybody has experience with fixation and embedding plant tissues in technovit? Could you email me with some details please....thanks in advance, Adam. ''~`` ( o o ) _____________________.oooO--(_)--Oooo._______________________________________ Adam Vivian-Smith PhD Student University of Adelaide/CSIRO Voice: +61 08 8303 8627 Division of Horticulture Fax : +61 08 8303 8601 Urrbrae, Adelaide Email: Adam.Vivian-Smith-at-adl.hort.csiro.au S.A., 5064 AUSTRALIA .oooO ( ) Oooo. ________________________\ (____( )_________________________________________ \_) ) / (_/
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I am a professional electrologist, permanent hair removal specialist. I am trying to find a way to determine the effect of high frequency current [heat] and galvanic action [alkali ] on pre- keratinized cells of the hair follicle. These currents are delivered to the lower part of the hair follicle via micro needle, the hair is then extracted by forceps. The lower part of the hair shaft contains matrix cells that are undifferentiated and capable of mitosis. These cells are available for analysis. Are there any microscopic techniques and \ or stains that will differentiate denaturated cells from ones in the normal state?
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At 4:24 PM 12/10/96 -0800, Ginger Baker wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Ginger, Once you filter the suspension of bacteria through a 0.2um polycarbonate filter, remove the filter from the housing and place it into fixative (2.5% glutaraldehyde in a buffer)} Follow this with: buffer washes, an optional post-fixation (osmium tetroxide) buffer washes gradient alcohol series (3X100% ETOH) At this point you have 2 options; 3 washes in HMDS (hexamethyldisilizane) followed by air-drying or Critical Point Drying.
Fix the filters to the SEM stub with double-sided sticky tape, add a drop of silver dag to the edge of the filter and sputter-coat.
Additional tips---during the intial stage of filtering, remove the syringe from the filter housing, pull plunger back, reattach and push a column of air through the housing---this pushes all of the filtrate through the filter so that it doesn't drain off when removing the filter from the Swinney holder. -------------------the filter housing and filter can be autoclaved ahead of time and sterile 1 cc. syringes used to insure a reliable prep. Best of luck, Rosemary Walsh
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At 16:24 10.12.1996 -0800, you wrote: Hi Ginger, =20 } I have an environmental engineer who is interested at looking at=20 } bacterial samples that have been filtered. He wants to look at the=20 } bacteria on the filters themselves. Does anyone know how to process= =20 } such samples? Do I let the filters air-dry or should I fix,=20 } dehydrate, and CPD the filters? Any help would be much appreciated.
I would definitely recommend to dehydrate and CPD the filters with the bacteria. After they are dry, you can cut the filter for suitable size to be mounted on a SEM-stud, whereafter they have to be coated for= conductivity. Good luck and happy holidays
Jouko
Jouko K. M=E4ki, Ph.D., Laboratory Manager University of Turku, Laboratory of Electron Microscopy Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND Tel: + 358 2 333 7318 GSM: + 358 40 505 2521 FAX: + 358 2 333 7380
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G'day all
Can I make a request to all those of you reply to questions. Take a moment to edit out extraneous text from your reply (yes I know that I'm responsible for some of that text aka the new "banner"). But it is certainly true that you do not have to "echo" or repost the entire message that you are replying to. Cut/Edit out the irrelevant parts and send only enough of the text so that the reader can get the gist of what you are replying to. This little courtesy will certainly make everyone's mail boxes a bit less cluttered and also save space in the archives.
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OCT stands for Optimal Control Temperature fluid (polyvinyl alcohol and polyethylene glycol) and is an embedding medium for frozen tissue specimens.
********************************************************* Sverker Enestrom M.D., Ph.D. Department of Pathology University of Linkoping, Sweden Phone: +46 13 22 15 20 Fax: +46 13 13 22 57 *********************************************************
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Dear Don
My predecessor here had a lot of problems using watyer soluble Durcupan resin. He manifested what we now realise was contact dermatitis, with skin blemishing, cracking and peeling. He was also sensitive to the cured blocks, at least certainly to their dust (we used to cure in ice cube trays and then cut specimens out with a hacksaw for mounting prior to ultramicrotomy as routine).
With best wishes - Keith Ryan
++++++++++++++++++++++++++++++++++++++++++++++++++ Keith Ryan B.Sc., Ph.D., C.Biol., M.I.Biol. Plymouth Marine Laboratory, Citadel Hill, Plymouth, Devon PL1 2PB, England
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Microscopy & Analysis (US Edition Issue No. 21, November 1996) ran an article you might be interested in - "Cryo-preparation of small or lightly attached biological specimens" by Robinson et al.
Basically, the idea is a variation on cryo-fixation - this normally involves plunging a specimen into liquid N2. In the case of specimens similar to yours, the result is that all the particles of interest drop off! However, if you simply fix the specimen on to a stub at room temperature and then transfer it to the pre-cooled cryo-stage, freezing is still relatively rapid. Obviously, you still loose internal specimen detail because of ice crystal growth but a considerable amount of external detail is successfully preserved. The authors present a number of good SEM images. Whether the procedure will work successfully with specimens as small as bacteria, I'm not sure.
If you want further details, contact Ken Robinson at KRO-at-pcmail.nerc-bas.ac.uk.
Regards,
--------------------------------------------------------------- Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS Technesis 17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk Uckfield, E. Sussex Phone: +44 (0)1825 766911 TN22 2AT Fax: +44 (0)1825 766911 United Kingdom ---------------------------------------------------------------
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Agar Scientific in the UK (fax +44-1279-815106) sell 75 mesh carbon composite grids. I'm sure SPI sell something similar in the US - check their web site. Both will be able to give you details of fabrication.
As to applications, I guess the two main applications would be for EDX analysis, to reduce background counts, and where specimen preparation procedures would bring the grid into contact with something that could corrode material such as Cu.
You might also want to consider carbon-coated nylon grids (but watch for Ti contamination), Be, Ti and Al, depending on your application.
(I have no commercial interest in Agar, SPI, or other consumables suppliers)
Regards,
--------------------------------------------------------------- Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS Technesis 17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk Uckfield, E. Sussex Phone: +44 (0)1825 766911 TN22 2AT Fax: +44 (0)1825 766911 United Kingdom ---------------------------------------------------------------
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Don
have you got this reference? It seems to be a key one referring to several others:
Tobler, M. and Freiburghaus (1990); Occupational risks of (meth)acrylate compounds in embedding media for electron microscopy Journal of Microscopy 160: 291-298
Good luck in your search.
Malcolm Haswell University of Sunderland UK e-mail es0mhs-at-environment.sunderland.ac.uk
----------
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Dear Colleagues:
One of our customers has had a rather strong allergic reaction during the handling and use of two embedding media simultaneously. One of the products was Unicryl and the other, Technovet. I am very concerned about the prevalence of such allergic reactions with acrylates, and am presently attempting to gather all pertinent literature and/or subscriber experiences with acrylate allergies.
A literature search on MEDLINE has revealed only one paper describing contact dermatitis reaction to Lowicryl, but many papers concerning the allergies to methacrylates used as adhesives by the dental profession and for cosmetic purposes.
I would appreciate learning of any experiences of the members and to receive any pertinent references regarding allergies to acrylate embedding media.
Regards, Don Cox ******************************************************** Donald P. Cox, Ph.D., M.B.A. GOLDMARK BIOLOGICALS/D.P. COX ASSOCIATES 437 Lock Street, Phillipsburg, NJ 08865-2764 (908) 859-2631 - - (908) 859-2875-FAX E-Mail: goldmrkr-at-fast.net/goldmarker-at-aol.com Web Page: http://members.aol.com/goldmarker
~~~"Goldmarking is everlasting probing!"~~~ ********************************************************
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What are the advantages/disadvantages (including price) in using the reusable TEM phosphor plates vs. a dedicated CCD camera system for collecting images?
Thanks.
************************************************************************** Lucille A. Giannuzzi, Ph.D. phone: 407 823-5770 University of Central Florida fax: 407 823-0208 Dept. of Mechanical, Materials, and Aerospace Engineering PO Box 162450 4000 Central Florida Blvd. Orlando, FL 32816-2450 email: lag-at-pegasus.cc.ucf.edu **************************************************************************
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Don, I don't know what to tell you except that this allergy is common, I am severly allergic to Lowicryl, and have been told that if I'm exposed to it again on my hands, I may lose the use of my hands. It took me 6 months to get the feeling back in my finger tips, and my fingers were so bad they were purple black. Also, the fumes from methacrylates etc is of no help to my chronic asthma.
I usually run into someone with the same problem in the Histology / EM field where ever I go.
We ordered the only gloves we found impermiable, H-4 Gloves from Monsanto, but they are very ackward to work in.
Lou Ann
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*************************** Lou Ann Miller Service Supervisor Center for Microscopy and Imaging College of Vet. Medicine University of Illinois 2001 S Lincoln Ave Urbana,Illinois 61801 217-244-1566 lamiller-at-ux1.cso.uiuc.edu
Microscopy Home Page: http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html
Central States Microscopy Society http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/CSMS/csms.html
Personal Home Page: http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/LAM.html
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You presumably have a bacteriology group in your vet school. Ask them about acridine orange or DAPI as stains for bacteria on memebrane filters. Rob Palmer CEB/UT
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I would try to minimize handling as much as possible to avoid losing any bacteria. We have resorted to exposure to osmium vapors for an extended period such as 24-72 hours and then air drying. At 04:24 PM 12/10/96 -0800, you wrote:
} Hello, } } I have an environmental engineer who is interested at looking at } bacterial samples that have been filtered. He wants to look at the } bacteria on the filters themselves. ******************************************************* G.W. Erdos, Ph.D. Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/
***** "Many shall run to and fro, and knowledge shall be increased" Daniel 12:4
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We can furnish the following: No. 25510: Carbon Polymer Composite Grid by CRI, 75 mesh. Low x-ray background. $6.75 each
No. 25500: EFFA Carbon Coated Nylon Grid, 180 mesh, Coated heavily on both sides with carbon. Low x-ray background. Also good for backscattered imaging. $5.00 for vial/25
see page 38 in 1992/3 catalog
We also sell thin-film substrates No. 11250 Carbon Films on 200 mesh copper grids $2.90 each; vial/100 $260 Special films are also made to order
We also have No. 14570: Carbon Mesh A fine lacy carbon film for use as a substrate in cases where a continuous solid substrate background would obscure the specimen. These come on 200 or 300 mesh copper grids.
Dianne
} } Hello, } } I looking for information about carbon TEM grids. } I would like to find out the following: } } typical mesh size } fabrication process } manufacturer } applications } } Anything you can tell me, or any reference, would be quite useful. Thanks in advance. } Please respond to the following email address: } oschueller-at-gmwgroup.harvard.edu } } Olivier } Ernest F. Fullam, Inc. Phone: (518) 785-5533 FAX: (518) 785-8647 E-Mail: pdf-at-fullam.com
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Hi all, I am also interested in the informations asked by Adam, about the use of Technovit resin for plant material. Would you please send them also for me? Could someone help me in another question? How do you find a good protocol for freeze-substitution of plant material (hydrated and desiccated leaves)? I have tryed three substitution solutions and some substitution-times between 2 and 12 weeks, but the results were good for just one species, not for the other two. What do you use as substitution-solution? And for subst-time? Thank you in advance! Estela ____________________________________________________
Estela S. Rossetto Ph.D. Student Departamento de Biologia Celular Instituto de Biologia - Unicamp Universidade Estadual de Campinas C.P. 6109 13081-970 Campinas SP Brasil Tel:(019) 2397821 Fax:(019) 2393124 e-mail: rossetto-at-obelix.unicamp.br ____________________________________________________
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Hi, I think it stands for "Optimum cutting temperature". At lease that is what I was told.
Bob Underwood Morph Core Lab U of Washington
On Tue, 10 Dec 1996, Glenn Holm wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } ------------------------------------------------------------------------ } . } } Somebody asked. The stuff one uses for cryostat cutting. What does OCT stand } for? }
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Dear Don,
I have personal experience with methyl methacrylate contact allergies. Years ago, when I was taught how to embed and section JB-4 (from Polyscienses), I was told that the solutions could cause a poison ivy-like reaction if I got it on my skin. My teacher, however chose not to were gloves, so neither did I. After a couple of months of continuous use, I developed the worst reaction that you could imagine. The fingers that had been in contact with the liquid form of the acrylic started to itch and burn, deep inside the flesh. Next, my fingers swelled to the size of a meaty hotdog and itched like crazy. Any attempt to even touch then sent sharp pains through my hand. I took antihistamines as soon as the reaction started and applied ointment for two days before the reaction was over. After that episode, I accidently touched the solid form once or twice and scrubbed my hands immediately and at length And took some more antihistamines. Still, I got a minor reaction that lasted a day each time. Now, about 10 years later, I still use JB-4 for alot of our samples, but I Always were gloves, and glasses when I'm arround it and I always wipe down work surfaces when I'm finished for the day. I have not had another even minor reaction for at least the last 5 years and never one as bad as the first. The reactions to these acrylics can be quite painfull, but many of the other embedding medias available are carcinogenic, with no early warnings. At least with this material, I know immediately when I've gotten too careless with my safety precautions.
Karen Pawlowski
On Tue, 10 Dec 1996, Donald P. Cox wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -------------------------------------------------------------------------. } } Dear Colleagues: } } One of our customers has had a rather strong allergic reaction during the } handling and use of two embedding media simultaneously. One of the products } was Unicryl and the other, Technovet. I am very concerned about the } prevalence of such allergic reactions with acrylates, and am presently } attempting to gather all pertinent literature and/or subscriber experiences } with acrylate allergies. } } A literature search on MEDLINE has revealed only one paper describing } contact dermatitis reaction to Lowicryl, but many papers concerning the } allergies to methacrylates used as adhesives by the dental profession and } for cosmetic purposes. } } I would appreciate learning of any experiences of the members and to receive } any pertinent references regarding allergies to acrylate embedding media. } } Regards, Don Cox } ******************************************************** } Donald P. Cox, Ph.D., M.B.A. } GOLDMARK BIOLOGICALS/D.P. COX ASSOCIATES } 437 Lock Street, Phillipsburg, NJ 08865-2764 } (908) 859-2631 - - (908) 859-2875-FAX } E-Mail: goldmrkr-at-fast.net/goldmarker-at-aol.com } Web Page: http://members.aol.com/goldmarker } } ~~~"Goldmarking is everlasting probing!"~~~ } ******************************************************** } } }
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I sent you a message just few minutes ago asking you to resend your message. I misquoted the version of my WordPerfect. It is a WP 6.1 not 7. Sorry.
Ann Fook
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Please post the following advertisement for an academic position at The University of Calgary for a Structural Biologist/Microscopist.
STRUCTURAL BIOLOGIST
The University of Calgary Department of Anatomy invites applications for a fulltime academic position as a structural/cell biologist. This position offerss an excellent opportunity for independent and collaborative research with other structural biologists employing technologies such as magnetic resonance spectroscopy and x-ray diffraction in the university-wide structural biology group, and for access to the University s Microscopy and Imaging Facility, comprising state-of-the-art electron and computer-based light microscopies. Duties will also include undergraduate teaching and graduate student supervision.
Qualifications include a PhD or equivalent, at least two years of postdoctoral experience, and a proven record of excellence in a research program which includes the development of advanced imaging techniques. Researchers particularly encouraged to apply are those with interests at the cellular or molecular level in an area complementing activities of a Faculty of Medicine research group such as Cancer Biology, Molecular & Developmental Biology, Joint Injury & Arthritis, etc. More information is available at http:/www.ucalgary.ca/~resoff/index.html.
The successful candidate must compete successfully for salary and establishment grant support from the Alberta Heritage Foundation for Medical Research and/or the Medical Research Council, and will have 75% of time protected for research.
In accordance with Canadian immigration requirements, priority will be given to Canadian citizens and permanent residents of Canada. The University of Calgary is committed to Employment Equity.
Please submit a curriculum vitae and statement of research and goals, and arrange for three letters of reference to be sent directly, by January 30, 1997, to:
Dr. D. Bazett-Jones Chair, Search Committee Department of Anatomy 3330 Hospital Drive Calgary, AB T2N 4N1, Canada TEL: (403) 220-3025, FAX: (403) 270-0737
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Some bacteria can stand airdrying. I suggest trying this with part of the sample. If the bacteria or other organisms look deflated you can CPD the remainder.
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Ginger:
I have done similar preps while at UMASS. Are the bacteria already on the filters? There are several kinds of filters. Some are better than others for SEM. The best ones to use for SEM are polycarbonate (Nuclepore type, Bio-Rad also sold some). Other ones are a torturous path type and it will be hard to find the bacteria on them.
Your best bet if there is a ?? is to look at an unused filter so you know what the background is. Polycarbonate ones are smooth with round holes in them. They come in several pore sizes. You will probably want ~.2um for bacteria. Too many bacteria will clog them up and stop the flow. My guess is these are some type of water filters. Certainly try to get a control to look at.
By all means fix the samples, osmicate, ethanol dehydrate, CPD and Au/Pd coat for best results. Fixing should also aid in keeping the bacteria in place. You can try air drying for comparison but the morphology will be poor. It depends on what your final purpose is; bacterial counts or being able to identify types and have nice morphology. I would recommend running a test batch first to be sure there are no problems. Feel free to call if you have any ???
Is Bill Chissoe at Stillwater?
Best of luck
Ed Basgall, PhD Penn State Univ. Dept. of Chemistry University Park, PA 16802 Ph: 814-865-0493
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I have seen several people propose that OCT stands for Optimal Cutting Temperature. That doesn't seem to make sense inasmuch as OCT is used at lots of different temperatures. My guess is that it stands for OPTIMAL CUTTING TEXTURE.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (573)-882-4712 (voice) (573)-882-0123 (fax)
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The phosphor plates have (potentially) a higher resolution than currently affordabel CCD devices. Actually, at the last price (I haven't seen the latest plate prices) I saw for the plate system you could probably afford a 2kx2k CCD camera. The camera is quicker but 2k is just bearly good enough if you expect to use the images like you are accustomed to, i.e. like film. A 2k image blown up to 8" is only 250 dpi - not very good. Even an average laser printer will do 600 dpi. The images will be OK if printed with a dye sub printer (300dpi) but you probably will not be able to enlarge sub areas of the image and get what you would expect from film. Area array CCD's bigger than 2k are still rare.
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I need to fix, stain, embed and section experimental collagen gels (not animal tissues) for TEM.
I'm looking for specific fixation, staining and dehydration advice. I thought I'd try buffered glutaraldehye, buffered osmium tetroxide for starters. Any advice as to concentrations, buffers, pH? For dehydrating, is either acetone or ethanol preferred?
As for staining, apparently uranyl acetate stains collagen intensely (Hayat, Positive Staining, p. 37). Should it be used after washing out fixatives before beginning dehydration, during dehydtation (eg. 50% ethanol or acetone), or only as a post-stain on sections?
Thanks for any help you can give.
Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
"When the mode of the music changes, the walls of the city will shake." - PLATO "There's a whole lotta shakin' goin' on!" - CHUCK BERRY
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We have in our lab a local computer network based on TokenRing. To link our old Link AN10/PDP11 computer to that network, we use a DMA channel. So this configuration works with a high performance bi-directional 16 bit parallel DR11-W compatible DMA channel for IBM PC-AT and compatible. The name of the board: XA-240 from Electrograph.
Since two weeks this link is down and we believe that this is related to the failure of the DMA board in the PC.
So we're looking for a used XA-240 board to evaluate if the problem is related to that board or to the Link AN10. If possible we will replace our board to repair that part of our network.
If anyone knows a way to get a board like this please let me know ASAP!
Eric Baril Centre for Characterization and Microscopy of Materials, (CM)2 Ecole Polytechnique de Montreal 2900, Edouard-Montpetit Montreal (Quebec) Canada H3C 3A7 Tel. (514) 340-4788 Fax. (514) 340-4468
Message-Id: {199612111900.OAA04365-at-ns1.axs2000.net} To: "microscopy-at-Sparc5.Microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}
-- [ From: Blackwood, Andrew * EMC.Ver #3.0 ] --
11 December 1996
Greetings:
Olivier Schueller asked about carbon grids. There are actually several types available, including "aperture" grids, which have single, large holes in the middle, 75 mesh pyrolytic carbon grids and carbon-coated nylon mesh grids. It all depends on what you are trying to do.
All of these are described in detail on our web site.
Disclaimer, all of these products are available from my employer, Structure Probe/SPI Supplies. Obviously, I have an interest in promoting their use.
Andy
Andrew W. Blackwood, Ph.D. Structure Probe, Inc. P.O. Box 656 West Chester, PA 19381-0656 Ph: 1 610 436 5400 FAX: 1 610 436 5755 e-mail: ablackwood-at-2spi.com WWW: http://www/2spi.com
Olivier Schueller asked about carbon grids. There are actually several types available, including "aperture" grids, which have single, large holes in the middle, 75 mesh pyrolytic carbon grids and carbon-coated nylon mesh grids. It all depends on what you are trying to do.
All of these are described in detail on our web site.
Disclaimer, all of these products are available from my employer, Structure Probe/SPI Supplies. Obviously, I have an interest in promoting their use.
Andy
Andrew W. Blackwood, Ph.D. Structure Probe, Inc. P.O. Box 656 West Chester, PA 19381-0656 Ph: 1 610 436 5400 FAX: 1 610 436 5755 e-mail: ablackwood-at-2spi.com WWW: http://www/2spi.com
I have a need for an unusual standard to be used on a microprobe (WDS) for quantitative trace element work. I need a carbonate with low quantities (e.g., 0.5-2% by weight) of SrO. Preferably the standard would be homo- geneous and the composition known accurately.
If anyone can point me in the right direction to track such a standard down, I'd be very grateful.
Karin E. Limburg Institute of Ecosystem Studies Millbrook, NY 12545
p.s. the application for which this standard is desired is to measure trace quantities of Sr in fish ear-stones.
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Hi All
I think I have found the mysterious problem that has affected some of you in the last 12 hours. At one point the new header started printing a "." at the start of a line. Some Email systems read this "dot" as end of message and then truncate the remaining posting.
If you are still experiencing problems please Email me direct. at
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} I have an environmental engineer who is interested at looking at } bacterial samples that have been filtered. He wants to look at the } bacteria on the filters themselves. Does anyone know how to process } such samples? Do I let the filters air-dry or should I fix, } dehydrate, and CPD the filters? Any help would be much appreciated. } } Thank you in advance, } } Ginger Baker
If the samples haven't already been filtered, then first sputter coat the filters before use, or use silver filters. Fewer imaging problems that way. With the bacteria on the filters, fix, dehydrate, and dry from hexamethyldisilizane at room temp or 60 C in a fume hood! I usually get excellent results this way. (Note: check Crang and Klomperens "Artefacts in Biological EM", title approximate, about fixing and dehydrating and their effects on bacterial coats. The slime coat may be important, and it's usually lost.) Phil
&&& Illigitimi non carborundum &&&&&&&& Philip Oshel Microscopy Station A PO Box 5037 Champaign, IL 61825-5037 (217)244-3145 days (217)355-3145 evenings oshel-at-ux1.cso.uiuc.edu *** looking for a job again ******************
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At 11:34 AM 12/11/96 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
2.5% glut should be fine and 1% OsO4 is all you need. But for dehydration using ethanol would be best.
} As for staining, apparently uranyl acetate stains collagen intensely (Hayat, } Positive Staining, p. 37). Should it be used after washing out fixatives before } beginning dehydration, during dehydtation (eg. 50% ethanol or acetone), or only } as a post-stain on sections?
I think if you stain with 2%aq. UA before starting the dehydration(but definately after throughly washing the buffer out) then there is very little chance that it would stain intensly. alcoholic ua tends to stain darker. This will give a chance to decide if post embedding staining is needed.
} Thanks for any help you can give. } } } Gib Ahlstrand, MMS Newsletter Editor } Electron Optical Facility, University of Minnesota, Dept. Plant Pathology } 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 } 612-625-9728 FAX, giba-at-puccini.crl.umn.edu } } "When the mode of the music changes, the walls of the city will shake." - PLATO } "There's a whole lotta shakin' goin' on!" - CHUCK BERRY } } } Regards... :-) :-) :-) :-) :-) :-) :-) :-) :-) :-) :-) :-) Visit us at http://www.med.upenn.edu/~path/core/EMCMAIN1.htm
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i do not recall her name off hand, but if you contact rmc,inc tucson,arizona,(they probably have a web page) and ask who was their biological instructor at their ultra microtomy conference this year in oct . I specifically remember, and i was surprised that she repeatedly cautioned the group about similar severe allergy-proned individual-reactions being rather common. I believe she also may be linked to this newsgroup and may reply directly. i believe she was retired and now consulting from her home in the Stanford area. when i can get to my records i'll try to remember to recover her name and send her e-mail address. i'm quite bad with names. On Tue, 10 Dec 96 18:03 EST "Donald P. Cox" {goldmrkr-at-fast.net} writes: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America
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} } } } Ginger Baker wrote: } } -------------------------------------------------------- } } I have an environmental engineer who is interested at looking at } } bacterial samples that have been filtered. He wants to look at the } } bacteria on the filters themselves. Does anyone know how to process } } such samples? Do I let the filters air-dry or should I fix, } } dehydrate, and CPD the filters? Any help would be much appreciated. } } -------------------------------------------------------- } } These kinds of samples really do have to be critical point dried, if } } examination is contemplated in a "conventional" SEM. In some instances, } } using silver membranes makes it possible to get away with looking at the now } } CPD's bacteria with far less or no metallization, an advantage in some } } situations. More information about silver membrane filters can be found on } } our website given below. } } } } Chuck } } } } ===================================================== } } Charles A. Garber, Ph. D. } e-mail:cgarber-at-2spi.com } } PRESIDENT } } SPI SUPPLIES/STRUCTURE PROBE, INC. } } WEST CHESTER, PA 19381-0656 USA } } } } Look us up at } } xxxxxxxxxxxxxxxxx } } http://www.2spi.com } } xxxxxxxxxxxxxxxxx } } ====================================================
Message-Id: {1.5.4b12.16.19961212081057.32a7a758-at-mail.mnet.fr} X-Sender: larnould-at-mail.mnet.fr X-Mailer: Windows Eudora Light F Version 1.5.4b12 F0.2 (16) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
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Hi all, I would like to thank all the people who answer to my question about LazarPrinter system. It was very helpfull. I can attach the summarize of the answers to all interresting persons.(If too much I'll directly mail on the listserver). Merry Xmas and happy new year to everybody. ========================================================== Jacky Larnould tel 33 (0)4 67 72 28 26 fax 33 (0)4 67 79 54 90 email larnould-at-mnet.fr
Message-Id: {199612120904.EAA12698-at-ns1.axs2000.net} To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
The following is a verbatum copy of part of a letter that was sent to SPI Supplies by Monsanto, dated December 3, 1996:
Dear Cutomer:
Monsanto has made a decision to exit the plyphenyl ether business: OS-124, OS-138 and Santovac 5 products by the end of the first quarter, 1997. We are continuing to seek a buyer who would be capable of producing this complex chemistry and will keep you informed of our progress. We want to signal you now so you can minimize the disruption this transition may have on your business. ------------------------------------------
The rest of the letter details how remaining stocks will be allocated to their distributors.
The letter ends with the following statement: If we receive sufficient requests for volume and we have not identified a suitable buyer for the business, we will schedule manufacture during 1997.
As a long time distributor, we are sorry to see Monsanto leaving this business. While the recent trend in EM has been away from diffusion pumps, there are still a large number of EMs that run on Santovac 5.
The purpose of this posting is not to cause a stampeed to the order departments but to make sure that there is adequate disclosure of this information within the markets we (e.g. SPI) serve. We do not know any more than what was stated in the December 3 letter. While we don't anticipate that there would be a complete ceasation of the production of Santovac by anyone, we do see the real possibility that there could indeed be a period of shortages. Our best advice is to keep a spare bottle on the shelf.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ =====================================================
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W.M.Nichols wrote:
} PLEASE BE SUSPICIOUS ABOUT THE REGIONAL NEWSPAPER REQUEST } I smell the possibility of SPAM } W.Nichols
What is SPAM? -- Philip Koeck Karolinska Institutet Dept. of Bioscience Novum S-14157 Huddinge Sweden Tel.: +46-8-608 91 93 Fax.: +46-8-608 92 90 Email: Philip.Koeck-at-csb.ki.se
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In message Thu, 12 Dec 96 07:55:12 G+3, "W.M.Nichols" {GCP3198-at-KAAU.EDU.SA} writes:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } PLEASE BE SUSPICIOUS ABOUT THE REGIONAL NEWSPAPER REQUEST } I smell the possibility of SPAM } W.Nichols } Yeah, that was the silliest thing I've ever seen on this listserver. But what is SPAM? I mean besides the canned stuff. F.C. Thomas GSC (Atlantic) MicroAnalysis Facility P.O. Box 1006, Dartmouth, N.S. B2Y 4A2 (902) 426-4635
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At 09:10 AM 12/12/96 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Thanks
Michael
} MIKE SPECHT SPECTRA SERVICES, INC. 1653 East Main Street Rochester, NY 14609 (716)654-9500 1800-955-7732 Fax: 716-654-8426 Visit our Web site at http://www.frontiernet.net/~mspecht/
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At 17:17 11/12/96 EST, you wrote: } } I have a need for an unusual standard to be used on a microprobe (WDS) for } quantitative trace element work. I need a carbonate with low quantities } (e.g., 0.5-2% by weight) of SrO. Preferably the standard would be homo- } geneous and the composition known accurately. } Karin E. Limburg
} p.s. the application for which this standard is desired is to measure trace } quantities of Sr in fish ear-stones. ********************************* Aragonite, the lime stone from coral would qualify, e.g. it's a carbonate and has small amounts of Sr. I do not know about it's homogeneity within one sample. Jim Darley Probing & Structure (Microscopy Supplies & Accessories) PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313 A great microscopy site: http://www.ultra.net.au/~pns/
You might get a considerable amount of useful information out of Chapters 8 & 9 of the book "Experimental High Resolution Electron Microscopy" by J. C. H. Spence, Clarendon Press, Oxford Univ, 1981, ISBN 0-19-851365-8 (I believe there is a second edition, too)
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Hi All, I could not verify if this was a real occurence or propogated hearsay, but then, better safe than sorry.
cheers
Lucio } } } } Subject: Virus Alert } } Importance: High } } } } If anyone receives mail entitled: PENPAL GREETINGS! please delete it } } WITHOUT reading it. Below is a little explanation of the message, and } } what it would do to your PC if you were to read the message. If you have } } any questions or concerns please contact SAF-IA Info Office on 697-5059. } } } } This is a warning for all internet users - there is a dangerous virus } } propogating across the internet through an e-mail message entitled } } "PENPAL GREETINGS!". DO NOT DOWNLOAD ANY MESSAGE ENTITLED "PENPAL } } GREETINGS!" } } } } This message appears to be a friendly letter asking you if you are } } interested in a penpal, but by the time you read this letter, it is too } } late. The "trojan horse" virus will have already infected the boot } } sector of your hard drive, destroying all of the data present. It is a } } self-replicating virus, and once the message is read, it will } } AUTOMATICALLY forward itself to anyone who's e-mail address is } } present in YOUR mailbox! This virus will DESTROY your hard drive, and } } holds the potential to DESTROY the hard drive of anyone whose mail is in } } your inbox, and who's mail is in their inbox, and so on. If this virus } } remains unchecked, it has the potential to do a great deal of DAMAGE to } } computer networks worldwide!!!! } } } } Please, delete the message entitled "PENPAL GREETINGS!" as soon as you } } see it! And pass this message along to all of your friends and } } relatives, and the other readers of the newsgroups and mailing lists } } which you are on, so that they are not hurt by this dangerous virus!!!! } } } } ------ =_0_MIME_Boundary_5246.32a5c1cf.imqyy6m0.dcuh029.dcu.ps.net-- } }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr.Lucio Mule'Stagno MEMC Electronic Materials Inc University of Missouri -St.Louis Silicon Materials Research Group Physics Dept., 501 Pearl Dr., 8001 Natural Bridge rd., St.Peters, St.Louis MO 63376 MO 63121 tel: 314 279 5338 314 516 5931/3 fax: 314 279 5363 314 516 6152 email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu URL:http://newton.umsl.edu/~luciom ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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To everyone that offered suggestions of how to proceed with the preparation of powders for TEM, thanks. We will be trying in January. Now to get rid of this nasty sunburn from Durban beaches! You missed a great MSSA conference last week! To everyone, all good wishes for the festive season. Mike
Michael J Witcomb PhD Electron Microscope Unit University of the Witwatersrand Private Bag 3 WITS 2050 South Africa
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Greetings, Estela Rossetto asked about freeze substitution protocols for plant tissue. The "best" medium will depend on your application, ultrastructure or immunocytochemistry (or, gasp, both). We have had very nice results with arabidopsis roots freeze substituted in acetone with 1% dimethoxypropane, for 48h at about minus 80 C. The dimethoxypropane acts as a chemical water scavenger so no messy molecular sieve required. Suprisingly, there is no need to include a fixative with the acetone. We find acceptable results with ethanol too. We are doing immuno work at the light microscope level, so we have not analysed this with the rigor of electron microscopy.
And on the resin question, I might add that we enjoy embedding plant material in butylmethyl methacryalte, which is easy to use and is removable after sectioning, so is great for immunocytochem (again, at the light level).
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} I need a carbonate with low quantities } (e.g., 0.5-2% by weight) of SrO. Preferably the standard would be homo- } geneous and the composition known accurately. } ... } p.s. the application for which this standard is desired is to measure trace } quantities of Sr in fish ear-stones.
I have done Sr in carbonates, but have always used a celestite standard. Sorry I can't help you with a carbonate, but please let me know (off-list) if you find one. I assume you would like to verify how much Sr you can see?
Good luck, Alfred
----------------------------------------- Alfred Kracher akracher-at-iastate.edu http://www.public.iastate.edu/~akracher -----------------------------------------
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I AM STILL LOOKING FOR A SEG (SIDE ENTRY GONIOMETER STAGE, NOT A SPECIMEN HOLDER) FOR A JEOL TEM. ANY 100 OR 200 MODEL. WE ARE WILLING TO PURCHASE AT A RESEANONABLE PRICE IF NECESSARY. IF NECESSARY WE COULD PURCHASE THE ENTIRE TEM.
THANKS
MARK A. WALL LAWRENCE LIVERMORE NATIONAL LAB L-350 CHEMISTRY & MATERIALS SCIENCE DEPT. 7000 EAST AVE LIVERMORE, CA 94550
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Dear Jacky
I would be interested in a summary/collection.
With best wishes - Keith Ryan
++++++++++++++++++++++++++++++++++++++++++++++++++ Keith Ryan B.Sc., Ph.D., C.Biol., M.I.Biol. Plymouth Marine Laboratory, Citadel Hill, Plymouth, Devon PL1 2PB, England
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} i do not recall her name off hand, but if you contact rmc,inc } tucson,arizona,(they probably have a web page) and ask who was their } biological instructor at their ultra microtomy conference this year in } oct . I specifically remember, and i was surprised that she repeatedly } cautioned the group about similar severe allergy-proned } individual-reactions being rather common. I believe she also may be } linked to this newsgroup and may reply directly.
I'm that instructor. I don't have any specific medical information; just the sort of word-of-mouth stories that are appearing here (which I'm saving for next year's Materials Microtomy course!). I'd appreciate comments from knowledgable MDs. I know that I read a paper some years ago about similar reactions to the acrylic resins used to cement metal joints into bone in orthopaedic surgery...
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117 Caspar, CA 95420
Dr. Steven Barlow EM Facility/Biology Department 5500 Campanile Drive San Diego CA 92182-4614 phone: (619)594-4523 fax: (619) 594-5676 email: sbarlow-at-sunstroke.sdsu.edu website: http://www.sci.sdsu.edu/EM_Facility
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The following is directly from the CIAC (U.S. Department of Energy's Computer Incident Advisory Capability) web page, most recently updated December 6:
-----
The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an e-mail chain letter by claiming that it is a self starting Trojan that destroys your hard drive and then sends copies of itself to everyone whose address in in your mailbox. Reading an e-mail message does not run it nor does it run any attachments, so this Trojan must be self starting. Aside from the fact that a program cannot start itself, the Trojan would also have to know about every different kind of e-mail program to be able to forward copies of itself to other people. This warning is totally a hoax.
-----
I would propose that virus alerts should **not** be posted unless they are first checked with CIAC or a similar "in the know" location. What do other subscribers think about this?
The probability that any of us become aware of a virus before an agency like CIAC does is, I believe, vanishingly small. Fake alerts are clogging the net at an alarming rate these days, creating a "cry wolf" problem that actually makes real viruses more dangerous.
Alfred
----------------------------------------- Alfred Kracher akracher-at-iastate.edu http://www.public.iastate.edu/~akracher -----------------------------------------
Message-Id: {1.5.4b12.16.19961212171503.0ec73a72-at-mail.mnet.fr} X-Sender: larnould-at-mail.mnet.fr X-Mailer: Windows Eudora Light F Version 1.5.4b12 F0.2 (16) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
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At 07:55 12/12/1996 G+3, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} Yeah, that was the silliest thing I've ever seen on this listserver. But } what is SPAM? I mean besides the canned stuff.
Dear Frank, A "spam" is a widespread, unsolicited email or newsgroup posting. Usually these are advertisements, often the return address of the spammer has been concealed, so that angry replies just clog some poor innocent's mailbox. Yours, Bill Tivol
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Hi: I myself have through the years developed a reaction to acrylates. My field is polymers and I am frequently in contact with acrylates. The monomer is the problem not the polymer. Wear gloves and always work in the hood. If you do get exposed wash or shower as soon as possible. The reaction is cumulative and becomes worse with each exposure. Good luck
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I'm thinking of buying a new low power ( {50x) zoom OM for general inspection work prior to SEM work. One of the other labs here has a WILD M10. It's a great scope, but I wanted to look around some more. I've looked in many of the catalogs, etc. but I wanted to get user input as well.
If you were going to buy a scope today, what would you get? What would you avoid? What accessories would you get (i.e. ring lights, diffuse lighting, polarzing filters, etc.)?
I'd like only user input, no sales pitches.
Thanks ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman-at-rd.sylvania.com
Our web sites: www.sylvania.com www.siemens.com --
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I'm thinking of buying a new low power ( {50x) zoom OM for general inspection work prior to SEM work. One of the other labs here has a WILD M10. It's a great scope, but I wanted to look around some more. I've looked in many of the catalogs, etc. but I wanted to get user input as well.
If you were going to buy a scope today, what would you get? What would you avoid? What accessories would you get (i.e. ring lights, diffuse lighting, polarzing filters, etc.)?
I'd like only user input, no sales pitches.
Thanks ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman-at-rd.sylvania.com
Our web sites: www.sylvania.com www.siemens.com --
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I'm thinking of buying a new low power ( {50x) zoom OM for general inspection work prior to SEM work. One of the other labs here has a WILD M10. It's a great scope, but I wanted to look around some more. I've looked in many of the catalogs, etc. but I wanted to get user input as well.
If you were going to buy a scope today, what would you get? What would you avoid? What accessories would you get (i.e. ring lights, diffuse lighting, polarzing filters, etc.)?
I'd like only user input, no sales pitches.
Thanks ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman-at-rd.sylvania.com
Our web sites: www.sylvania.com www.siemens.com --
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Hello All!
Has anyone out there thin sectioned undecalcified bone embedded into epon/araldite? Please relay any information y'all might have, pointers, etc. on the best way to trim and section this stuff. Can we use a glass knife? Or does it only cut with diamond?
Thanks, in advance, for all your help.
Paula Sicurello U.C. Berkeley Electron Microscope Lab
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Hello everyone
I am sure that you all have received from time to time messages that were inappropriate to the listserve, e.g. the recent one about newspapers. As I can best recall, the response to this and other message by Nestor, our friendly sysop, was and has been swift and clear. May I suggest that by letting him handle it we can all save time, resources, bandwidth? (whatever that is :-)), etc. by not individually responding, usually with the original message included.
Seasons peace and joy to you all (that's PC for merry Christmas and happy New Year).
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TECHNICIAN POSITION JOHNS HOPKINS UNIV. IMAGING FACILITY
We have an opening for a Research Technician to operate and maintain imaging and fluorescence analysis equipment which includes a Zeiss LSM410 confocal microscope, low-light digital imaging microscope, fluorometers, and image analysis workstations. We are seeking an individual with strong computer skills (Windows programming experience highly desirable) as well as experience with advanced light microscopy and/or lasers and optics. Programming expertise is needed for designing and implementing new routines for both image acquisition and image analysis. The successful candidate will be responsible for training and assisting users from throughout Johns Hopkins University in studies which involve fixed or living samples, as well as performance of most administrative aspects of running the facility. The most challenging portion of the facility is the confocal equipment, so candidates with experience in confocal microscopy are highly encouraged to apply.
The position is full-time and is available immediately. Salary will be based on experience and education level of the successful applicant. Applications will be accepted until the position is filled.
Please send CV, salary requirements, and names of three references to Dr. Chip Montrose, Ross 930, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205. Alternatively, e-mail mhm3-at-jhu.edu or FAX 410- 955-9677.
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With regard to staining collagen gels, try adding 0.1% w/v tannic acid to the primary fix (aldehyde). This will improve the staining of the collagen remarkably.
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With regard to staining collagen gels, try adding 0.1% w/v tannic acid to the primary fix (aldehyde). This will improve the staining of the collagen remarkably.
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I would like to obtain a PCVISIONplus framegrabber (PAL version). These boards were very popular some years ago for monochrome image grabbing.
If you have one of these which you are not using and would be willing to part with it, would you please email me.
David Vowles Electron Microscopy Unit Australian National University PO Box 475 Canberra ACT 2601 Australia Tel:(616) 2493543 Fax:(616) 2494891 Email: David.Vowles-at-anu.edu.au
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} } Please forward this message to your scientific departments. } } } } The University of Arkansas has a Siemens Electron Microscope in good } } working order that we would like to surplus. The equipment could be } } obtained for little more than the cost of transporting & installation. } } } } Please contact me if there is any interest in this piece of } } equipment. Thank you. } } } } Jane T. Eaves, CPPO } } Business Manager } } 321 Administration Bldg. } } University of Arkansas } } Fayetteville, AR 72701 } } } } Phone 501-575-2551 } } Fax 501-575-4158 } } } } }
Dr. Steven Barlow EM Facility/Biology Department 5500 Campanile Drive San Diego CA 92182-4614 phone: (619)594-4523 fax: (619) 594-5676 email: sbarlow-at-sunstroke.sdsu.edu website: http://www.sci.sdsu.edu/EM_Facility
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Hello everyone
I am sure that you all have received from time to time messages that were inappropriate to the listserve, e.g. the recent one about newspapers. As I can best recall, the response to this and other message by Nestor, our friendly sysop, was and has been swift and clear. May I suggest that by letting him handle it we can all save time, resources, bandwidth? (whatever that is :-)), etc. by not individually responding, usually with the original message included.
Seasons peace and joy to you all (that's PC for merry Christmas and happy New Year).
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YES!!!
On Thu, 12 Dec 1996, Alfred Kracher wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } The following is directly from the CIAC (U.S. Department of Energy's } Computer Incident Advisory Capability) web page, most recently updated } December 6: } } ----- } } The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an } e-mail chain letter by claiming that it is } a self starting Trojan that destroys your hard drive and then sends copies } of itself to everyone whose address in in your } mailbox. Reading an e-mail message does not run it nor does it run any } attachments, so this Trojan must be self starting. } Aside from the fact that a program cannot start itself, the Trojan would } also have to know about every different kind of } e-mail program to be able to forward copies of itself to other people. This } warning is totally a hoax. } } ----- } } I would propose that virus alerts should **not** be posted unless they are } first checked with CIAC or a similar "in the know" location. What do other } subscribers think about this? } } The probability that any of us become aware of a virus before an agency } like CIAC does is, I believe, vanishingly small. Fake alerts are clogging } the net at an alarming rate these days, creating a "cry wolf" problem that } actually makes real viruses more dangerous. } } Alfred } } ----------------------------------------- } Alfred Kracher } akracher-at-iastate.edu } http://www.public.iastate.edu/~akracher } ----------------------------------------- } }
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I'm thinking of buying a new low power ( {50x) zoom OM for general inspection work prior to SEM work. One of the other labs here has a WILD M10. It's a great scope, but I wanted to look around some more. I've looked in many of the catalogs, etc. but I wanted to get user input as well.
If you were going to buy a scope today, what would you get? What would you avoid? What accessories would you get (i.e. ring lights, diffuse lighting, polarzing filters, etc.)?
I'd like only user input, no sales pitches.
Thanks ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman-at-rd.sylvania.com
Our web sites: www.sylvania.com www.siemens.com --
Here at IBM, there is a fairly extensive Industrial Safety and Hygiene program. In our training classes, it was explained to us that epoxies, etc., are "sensitizers" as are Poison Ivy, Poison Oak, and Poison Sumac. Some people may have a reaction on their first exposure. Others may have a reaction after repeated exposures have "sensitized" them. The reactions will most likely increase in sevearity with each additional exposure. There are a few that never show a reaction. I know people that were never affected by Poison Ivy when they were children, but had severe reactions when exposed as an adult.
Precautions during use (what we are required to do): * The resins and hardeners are stored in a vented cabinet. * Weighing, mixing, and sitting to cure are done in a vented hood. *** Protective Appearal *** *** Nitrile Gloves - eg. Nitrilite by Ansell Edmont Note: Latex gloves were not accepted * Goggles * Plastic Chemical Apron
Hope this helps.
Darrell Miles darrell_miles-at-vnet.ibm.com
IBM Analytical and Test Services http://www.chips.ibm.com/services/asg/
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} I'm thinking of buying a new low power ( {50x) zoom OM ... } ...I wanted to get user input as well. } ------------------------------------------------- } Harold J. Crossman } OSRAM SYLVANIA INC.
I've used a Zeiss SV8 for about 10 years and have no reason to even think of updating it. I chose it over the (then) Wild for it's large zoom range and interchangeable objectives (the old Wild's had horrible 'add-on' 2x objectives). It might be worth finding out whether that M10 in the other lab takes interchangeable or supplementary objectives.
Obviously, the choice of scope and accessories will depend on your work and budget. I lay out sub-millimetre mollusc radulae and then go about spreading the teeth on them. Indispensible accessories are; * fibre optics (rather than built-in light) to get low angles to make 'invisible' specimens cast a shadow, * side-action micrometre stage (for transferring REALLY small specs; I can grab a spec, lift it up while racking up the focus, use the stage to bring over the next container or stub, lower it while racking down, i.e. keeping my eyes firmly glued on the little sucker through the whole process), * 2x objective and 16x eyepieces (total mag of 205x - yes, rather fuzzy :-)
I also have a neat monocular phototube for it which slides from one light path to the other allowing sequential stereopairs.
I'm of the view that quality lasts and would automatically be drawn to Leica and Zeiss. Either of their top shelf models would be good; certainly the Zeiss SV11 that I've tried is a superb instrument. I have no financial interest in either but, as implied earlier, I've never regretted finding the extra dollars for my Zeiss.
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Hi I am related to sales but wont give you a sales pitch just advice. M10 is an APO type lens which mean that you won`t see those usually yellow fringes around the specimens but also probably the most expensive microscope. If you can afford it, it is the best. Now it depends of you and your applications, The first criteria would be to ask yourself what you exactly plan to do with it now and latter. 1)is resolution important? 2)do you observe,I think so, flat specimens if yes a Plan objective would be preferable.(it wont give you this dome or cavity effect when you observe flat specimen) 3)do you also need transmitted light observation like bright field and/or may be darkfield illumination.Polarisation is sometimes usefull to get rid of glare but it as to be tried. 4)Reflected light Illumation is very important. I would trend to look for fiber optic more than neon especialy for the brightness and versatility. You should try for ring light as well as flexible twin arm.If you plan to take color pictures neon light is not very good. 5)Will you spent a lot of time observing, if yes, a variable angle tube would be very usefull. 6)What is the lowest magnification that you feel comfortable to work with. Hig quality stereo are incline to aim for hihg magnifications not low. ,This is where you see realy a difference in resolution. at low power it is much more difficult to see it. Check if you are using a zomm if the brightness remain constant or acceptable when you zoom from low to high magnification. It is not very convenient if you have to adjust the light intensity when you zoom from low power to high. Finally why don`t you get in touch with some salesmen and ask for demo and purchase from the one you trust the most,give you the best advise and doesn`t give you any bullsheet and try to sell what meet your expectations and needs. Norm At 13:04 12/12/96 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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At 09:10 12/12/96 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
X-Authentication-Warning: umbc8.umbc.edu: prutle1 owned process doing -bs
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This is to serve notice,by order of the Chair, Dr. Lasse Lindahl, the Electron Microscopy Facility located at The University of Maryland Baltimore County,Biology Dept. will no longer be accepting contractual electron miocroscopy requests. I would like to thank all of the private companies, universities and federal and state agencies who helped to make this lab a success.
MERRY CHRISTMAS TO ALL AND TO ALL A GOOD NIGHT! HO-HO-HO
Peace through Christ,
Phil Rutledge, Director e-mail: prutle1-at-gl.umbc.edu Center for Microscopy voice: (410) 455-3582 UMBC Dept. of Biology fax: (410) 455-3875 Catonsville, MD 21228 ///// / / / / /////// /////// / / /////// /////// / / / / / / / / / / / / /////
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Looks pretty bogus to me, and a lot like the Good Times virus hoax. You still don't get viruses from reading e-mail. The closest you come is executing attachments without checking them for viruses.
My Eudora allows me to double-click on the attachment name and bring up the file. If it was a DOC file, then Word would be opened. If it was an executable, then it would run. That would not be good as I would risk infecting my computer. Even opening a Word file that way bypasses the protection that MS provided against macro viruses. I recall that that protection only worked if a document was accessed from File/Open.
At 09:12 AM 12/12/96 -0600, you wrote: } Hi All, } I could not verify if this was a real occurence or propogated hearsay, } but then, better safe than sorry. } } } } Subject: Virus Alert } } } Importance: High } } } If anyone receives mail entitled: PENPAL GREETINGS! please delete it ---------------------------------------------------- Warren E. Straszheim 270 Metals Development, Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091
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With regard to cutting bone, we fix as usual (1.5/1.5 w/ tannic acid; 1%OsO4; dehydrate and embed in Spurrs epoxy. We do not decalcify. Bone cuts with difficulty and will quickly trash a glass knife. Though bone will certainly decrease the life expectancy of a diamond, it seems the method of choice. A knife of a wider angle might help with the problem of edge deterioration, but we use standard knives in this lab.
Good luck, ---------------------- Doug Keene DRK-at-shcc.org
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With regard to cutting bone, we fix as usual (1.5/1.5 w/ tannic acid; 1%OsO4; dehydrate and embed in Spurrs epoxy. We do not decalcify. Bone cuts with difficulty and will quickly trash a glass knife. Though bone will certainly decrease the life expectancy of a diamond, it seems the method of choice. A knife of a wider angle might help with the problem of edge deterioration, but we use standard knives in this lab.
Good luck, ---------------------- Doug Keene DRK-at-shcc.org
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Hi Depend of the size of the specimen. I have a customer who use a saw microtome from Leitz to obtain bones sections of about 50 microns thick. (pigs bones) If you need her coordonates please let me know. Sections were used for photonic observations not EM. Norm At 12:20 12/12/96 -0800, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
X-Sender: jokamaki-at-mailhost.utu.fi X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable To: Microscopy-at-Sparc5.Microscopy.Com
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At 08:00 12.12.1996 -0800, you wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20 A } RESEANONABLE PRICE IF NECESSARY. IF NECESSARY WE COULD PURCHASE THE ENTIRE } TEM.
Mark,
My message from Nov. 28th is still valid.
Jouko
Jouko K. M=E4ki, Ph.D., Laboratory Manager University of Turku, Laboratory of Electron Microscopy Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND Tel: + 358 2 333 7318 GSM: + 358 40 505 2521 FAX: + 358 2 333 7380
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Confirmation of reading: your message -
Date: 12 Dec 96 17:29 To: microscopy-at-Sparc5.Microscopy.Com Subject: TEM powder prep - THANKS
Was read at 8:38, 13 Dec 96.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- J. B. Bilde-Soerensen Materials Department Risoe National Laboratory DK-4000 Roskilde Denmark
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Bevestiging van lezing : uw bericht -
Datum: 12 Dec 96 17:29 Aan: microscopy-at-Sparc5.Microscopy.Com Ondw.: TEM powder prep - THANKS
Gelezen om 8:41, 13 Dec 96.
----- | o | R.Bekenkamp | I | Hogeschool van Utrecht | I | Faculteit Natuur en Techniek | I | Afd. LCT --H-- Holland I Tel 030-2712404 I Fax 030-2730905 I E-mail R.Bekenkamp-at-lct.fnt.hvu.nl { }
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Please disregard for the present our notification of new e-mail address. (We had hoped to be micrick5-at-home.com by now, but have had an installation problem.) We are still getting messages via Earthlink address, altho we were supposed to have cancelled it as of Dec 10.
} What are the advantages/disadvantages (including price) in using the } reusable TEM phosphor plates vs. a dedicated CCD camera system for } collecting images? } } Thanks.
} Lucille A. Giannuzzi, Ph.D.
I am not an expert on this field, but I worked once with this kind of imaging plate system in Japan.
One very obvious difference to an CCD-array is the size of the imaging plate. It has the same format like an good old photo film. The size of a CCD-array is a few square-cm in the best case. Therefor you have to reduce the magnification if you want to record the image you saw on your final screen.
On the other hand, with a CCD you have direct access to the image data. An imaging plate must be processed in its reader first, a process that can take several minutes at best.
For all technical details (sensitivity, dynamic range, ...) I would recommend you to read
Mori, N et al.: Application of the "imaging plate" to TEM image recording. Ultramicroscopy 25 (1988) 195-202
or
Mori, N et al.: Development of the imaging plate for the transmission electron microscope and its characteristics. J. Electron Microsc. 39 (1990) 433-436
Petra Wahlbring
-------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public Centre Universitaire (CRP-CU) Laboratoire d'Analyse des Mat=E9riaux (LAM) 162a, av. de la Fa=EFencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpcu.lu or 100112.2335-at-compuserve.com
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The DOE-CIAC web site is at
http://ciac.llnl.gov/
and the hoaxes page
http://ciac.llnl.gov/ciac/CIACHoaxes.html
} The following is directly from the CIAC (U.S. Department of Energy's } Computer Incident Advisory Capability) web page, most recently updated } December 6: } } ----- } } The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an } e-mail chain letter by claiming that it is } a self starting Trojan that destroys your hard drive and then sends copies } of itself to everyone whose address in in your } mailbox. Reading an e-mail message does not run it nor does it run any } attachments, so this Trojan must be self starting. } Aside from the fact that a program cannot start itself, the Trojan would } also have to know about every different kind of } e-mail program to be able to forward copies of itself to other people. This } warning is totally a hoax. } } ----- } } I would propose that virus alerts should **not** be posted unless they are } first checked with CIAC or a similar "in the know" location. What do other } subscribers think about this? } } The probability that any of us become aware of a virus before an agency } like CIAC does is, I believe, vanishingly small. Fake alerts are clogging } the net at an alarming rate these days, creating a "cry wolf" problem that } actually makes real viruses more dangerous. } } Alfred } } ----------------------------------------- } Alfred Kracher } akracher-at-iastate.edu } http://www.public.iastate.edu/~akracher } -----------------------------------------
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In message Thu, 12 Dec 1996 12:08:10 -0500 (EST), William Tivol {tivol-at-wadsworth.org} writes:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } } } Yeah, that was the silliest thing I've ever seen on this listserver. } } But what is SPAM? I mean besides the canned stuff. } } } } Dear Frank, } A "spam" is a widespread, unsolicited email or newsgroup posting. } Usually these are advertisements, often the return address of the spammer } has been concealed, so that angry replies just clog some poor innocent's } mailbox. } Yours, } Bill Tivol } Thanks, Bill. There always seems to be some new jargon or slang expression to catch up on. F.C. Thomas GSC (Atlantic) MicroAnalysis Facility P.O. Box 1006, Dartmouth, N.S. B2Y 4A2 (902) 426-4635
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G'day Subscribers.....
Yes I have seen that, contrary to the rule, that h.smits-at-celbi.kun.nl has set his/her Email to *automatically reply* to each message. I have removed him/her from the Microscopy Listserver, until such time as that is fixed at his/her end.
I've not had any more reports of blank messages, so I am assuming that the problem is now cured.
For the record, will those of you that had the problem (i.e. receiving the banner only and then no message. Please send me an Email message I would like the following information (or as much as you can provide).
Your Email Address Your Email Service Provider (My Company, AOL, .....) & Their Computer System (i.e IBM Mainframe, Sun Server .etc....) & Their Service (Direct Login, POP3, SMTP.....) Your Email Program (Eudora, Pegasus, White Pine, Netscape......)
I'd like to see what the common characteristics were of the sites that had the problem as it was NOT universal.
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Harold,
As long as you're going to use an OM at ' {50X for general inspection work prior to SEM work' then any of the standard OM brands will suffice (we have a Wild M3Z in our SEM/TEM lab, do most of our pre-EM OM work in the 10-50X range, and are very satisfied with the Wild). More important (since you are doing, in effect, correlative microscopy) would be your illumination options - get lots. We use ring, coaxial, transmitted (bright and dark fields), and fiberoptic oblique (frequency of use in about that order). I find coaxial to be especially useful when going from the OM to the SEM. Also get a recording option (Polaroid, video printing).
At 01:04 PM 12/12/96 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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Paula,
We have cut undecalcified avian growth plate from the point where it is hyaline cartilage down to the actual bone. We always used Spurr resin, although E/A would probably work fine. Our sections were HUGE, measuring at least 2x2 mm and were mounted on slot grids. We used a new diamond knife which was dedicated to this project, assuming that it would get dull and scratchy quickly. After thousands of sections, it showed no more wear and tear than a diamond used for more mundane purposes. A glass knife probably would have sufficed. Unless you are working with a particularly dense bone, you shouldn't have any unusual problems.
-=W.L. Steffens=- Department of Veterinary Pathology College of Veterinary Medicine University of Georgia
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Harold;
My pre-EM microscope is actually a hybrid, made from parts of scopes we had around here. I combined a Nikon SMZ-2T (10-63x zoom and photo tube) with an Olympus stage/base to provide me with reflected, dark and bright field lighting. I am sure that most manufacturers can provide you with these options in a single scope; for me money was an issue at the time. The scope is inside of a laminar flow hood so that I can do relatively contaminant-free prep work. This seems to serve most of my needs quite well. My personal experience is that these are probably the minimum accessories you would require. I use a separate research grade scope (Zeiss) for specialized applications such as the use of polarizing filters, Nomarski, digital imaging, image analysis, etc.
Best Regards,
-Bob *********************************** Bob Citron Chiron Vision 555 W. Arrow Hwy Claremont, CA 91711 (909)399-1311 email: Bob_Citron-at-cc.chiron.com *********************************** ______________________________ Reply Separator _________________________________
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I'm thinking of buying a new low power ( {50x) zoom OM for general inspection work prior to SEM work. One of the other labs here has a WILD M10. It's a great scope, but I wanted to look around some more. I've looked in many of the catalogs, etc. but I wanted to get user input as well.
If you were going to buy a scope today, what would you get? What would you avoid? What accessories would you get (i.e. ring lights, diffuse lighting, polarzing filters, etc.)?
I'd like only user input, no sales pitches.
Thanks ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman-at-rd.sylvania.com
Our web sites: www.sylvania.com www.siemens.com --
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On Thu, 12 Dec 1996, Crossman, Harold wrote:
} I'm thinking of buying a new low power ( {50x) zoom OM for general } inspection work prior to SEM work. One of the other labs here has a
} you avoid? What accessories would you get (i.e. ring lights, diffuse } lighting, polarzing filters, etc.)?
Harold, I use our scope (Nikon) routinely and particularly like the fiber-optic light source, Lumina-I. This has the choice of a ring for even illumination of the smaple, or a two-pronged, bendable source to customize oblique illumination. This was purchased over five years ago, so I don't know current vendors, prices, etc. We also have ours fitted to a video camera. This is relatively inexpensive and gives you the options of thermal printers rather than film, image on a screen for viewing by a group, video-capture with computer, etc. I believe most microscopes now have the option of a tripod eyepiece, useful for adding the video camera.
Gary Chandler Materials Science and Engineering University of Arizona
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Thank you to all who replied to my query concerning examination of filter= s =
containing bacteria. Here are a list of the responses I received. =
Ginger Baker EM Lab Manager Dept. of Anatomy, Pathology, and Pharmacology 250 Veterinary Medicine Oklahoma State University Stillwater, OK 74078 (405) 744-6765 FAX: (405) 744-5275 Email: lizard-at-okway.okstate.edu =
=
(1) Some bacteria can stand airdrying. I suggest trying this with part o= f the =
sample. If the bacteria or other organisms look deflated you can CPD the= =
remainder.
Dave
(2) I have done similar preps while at UMASS. Are the bacteria already =
on the filters? There are several kinds of filters. Some are better tha= n =
others for SEM. The best ones to use for SEM are polycarbonate (Nuclepo= re =
type, Bio-Rad also sold some). Other ones are a torturous path type and = it will be hard to find the bacteria on them.
Your best bet if there is a ?? is to look at an unused filter so you know=
what the background is. Polycarbonate ones are smooth with round holes i= n them. They come in several pore sizes. You will probably want ~.2um for bacter= ia. Too many bacteria will clog them up and stop the flow. My guess is these= are some type of water filters. Certainly try to get a control to look at.
By all means fix the samples, osmicate, ethanol dehydrate, CPD and Au/Pd = coat for best results. Fixing should also aid in keeping the bacteria in plac= e. You can try air drying for comparison but the morphology will be poor. It depends on what your final purpose is; bacterial counts or being able to identify types and have nice morphology. I would recommend= =
running a test batch first to be sure there are no problems. Feel free to call if you have any ???
Ed Basgall, PhD Penn State Univ. Dept. of Chemistry University Park, PA 16802 Ph: 814-865-0493
(3) If the samples haven't already been filtered, then first sputter coat the filters before use, or use silver filters. Fewer imaging proble= ms that way. With the bacteria on the filters, fix, dehydrate, and dry from hexamethyldisilizane at room temp or 60 C in a fume hood! I usually get excellent results this way. (Note: check Crang and Klomperens "Artefacts in Biological EM", title =
approximate, about fixing and dehydrating and their effects on bacterial = coats. =
The slime coat may be important, and it's usually lost.) Phil
&&& Illigitimi non carborundum &&&&&&&& Philip Oshel Microscopy Station A PO Box 5037 Champaign, IL 61825-5037 (217)244-3145 days (217)355-3145 evenings oshel-at-ux1.cso.uiuc.edu
(4) } These kinds of samples really do have to be critical point dried, if=
} } examination is contemplated in a "conventional" SEM. In some instance= s, } } using silver membranes makes it possible to get away with looking at t= he now } } CPD's bacteria with far less or no metallization, an advantage in some=
} } situations. More information about silver membrane filters can be fou= nd on } } our website given below. } } } } Chuck } } } } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D } } Charles A. Garber, Ph. D. } e-mail:cgarber-at-2spi.com } } PRESIDENT } } SPI SUPPLIES/STRUCTURE PROBE, INC. } } WEST CHESTER, PA 19381-0656 USA } } } } Look us up at } } xxxxxxxxxxxxxxxxx } } http://www.2spi.com } } xxxxxxxxxxxxxxxxx
(5) I would try to minimize handling as much as possible to avoid losing = any bacteria. We have resorted to exposure to osmium vapors for an extended period such as 24-72 hours and then air drying.
G.W. Erdos, Ph.D. Phone: 352-392-1295 =
Scientific Director, = =
ICBR Electron Microscopy Core Lab = =
218 Carr Hall Fax: 352-846-0251 =
University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 http://www.biotech.ufl.edu/~em= cl/
(6) You presumably have a bacteriology group in your vet school. Ask the= m about acridine orange or DAPI as stains for bacteria on memebrane filters= =2E Rob Palmer CEB/UT
(7) If you have a filter to play with, I would first attempt to simply ai= r dry =
it, perhaps under laminar flow. I've done this before with samples that = were =
surface-innoculated with bacillus subtillus. Obviously, some of the =
bacteria were collapsed from the vacuum, but the majority of them were in= tact =
and made quite a nice SEM image when sputter coated with about 10 nm of A= u:Pd. Regards, -Bob ************************************ Bob Citron Chiron Vision 555 W. Arrow Hwy Claremont, CA 91711 (909)399-1311 Bob_Citron-at-cc.chiron.com
(8) I have looked at many bacterial samples,particularly sulfate reducers= , by =
both air drying and fixation, dehydration, and CPD of filters and often g= et =
similar results. If there is a capsule or extracellular material of intre= st the =
fix, dehyd, CPD route may be needed but why not air dry a piece of the fi= lter =
for a quick look.
(9) Once you filter the suspension of bacteria through a 0.2um polycarbon= ate =
filter, remove the filter from the housing and place it into fixative (2.= 5% =
glutaraldehyde in a buffer) follow this with: buffer washes, an optional post-fixation (osmium tetroxide) buffer washes gradient alcohol series (3X100% ETOH)
At this point you have 2 options; 3 washes in HMDS (hexamethyldisilizane) followed by air-drying or Critical Point Drying.
Fix the filters to the SEM stub with double-sided sticky tape, add a drop of silver dag to the edge of the filter and sputter-coat.
Additional tips---during the intial stage of filtering, remove the syringe from the filter housing, pull plunger back, reattach and push a column of air through the housing---this pushes all of the filtrate through the filter so that it doesn't drain off when removing the filter from the Swinney holder.
The filter housing and filter can be autoclaved ahead of time and sterile= 1 cc. =
syringes used to insure a reliable prep. Best of luck, Rosemary Walsh
(10) I would definitely recommend to dehydrate and CPD the filters with the bacteria. After they are dry, you can cut the filter for suitable siz= e to be mounted on a SEM-stud, whereafter they have to be coated for conduc= tivity. Good luck and happy holidays
Jouko
Jouko K. M=E4ki, Ph.D., Laboratory Manager University of Turku, Laboratory of Electron Microscopy Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND Tel: + 358 2 333 7318 GSM: + 358 40 505 2521 FAX: + 358 2 333 73= 80
(11) Microscopy & Analysis (US Edition Issue No. 21, November 1996) ran a= n article you might be interested in - "Cryo-preparation of small or lightl= y attached biological specimens" by Robinson et al.
Basically, the idea is a variation on cryo-fixation - this normally involves plunging a specimen into liquid N2. In the case of specimens similar to yours, the result is that all the particles of interest drop off! However, if you simply fix the specimen on to a stub at room temperature and then transfer it to the pre-cooled cryo-stage, freezing i= s still relatively rapid. Obviously, you still loose internal specimen deta= il because of ice crystal growth but a considerable amount of external detai= l is successfully preserved. The authors present a number of good SEM image= s. Whether the procedure will work successfully with specimens as small as bacteria, I'm not sure.
If you want further details, contact Ken Robinson at KRO-at-pcmail.nerc-bas.= ac.uk.
Regards,
--------------------------------------------------------------- Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS Technesis 17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk Uckfield, E. Sussex Phone: +44 (0)1825 766911 TN22 2AT Fax: +44 (0)1825 766911 United Kingdom
(12) Use ESEM ( environmental scanning electron microscope) equipped with= a cold stage going down to 1-2 degrees C. You will be able to use wet filter wit= h a =
bacterial deposit on it and to dry carefully water out while in the =
microscope. For a short time ~ 5 minutes, you should be able to see and =
identify your bacteria without substantial distortion. Alternatively, fix= =
them with 1% of OsO4 in water while on filters and repeat as above. Use 2= 0 =
kV and high condenser setting (60-70%) for artefact free observation. Cheers, Wis Jablonski OiC EM/X-ray Microanalysis, CSL, University of Tasmania
(13) I would fix, dehydrate etc. just as you would for any biological spe= cimen.
Regards, Marilyn Henderson CEMMSA The University of Adelaide South Australia
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Harold, We purchased an Olympus SZ60 with a 45 deg. prism for SZ stereo, 1X-6.3X, 10X eyepieces + a doubler lens, 2.5 X photoeyepiece, iris light source, dual flexible gooseneck fiberoptic illumination with focus heads. We added an extension pillar to obtain 100mm working distance. We've interfaced this with a CCD-TV camera, monitor and color printer as well as an adaptor for a 35mm Pentax thread. We dealt with Dave Williams from HiTech Instruments (610) 353-3505 and found him knowledgeable, able to address our needs and budget constraints. Rosemary
#################################################### Rosemary Walsh Electron Microscope Facility for the Life Sciences The Biotechnology Institute for Research and Education 1 South Frear Lab University Park, PA 16802 814-865-0212 email:rw9-at-psu.edu ####################################################
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Can anyone provide the name of a US dealer for the phospor plates?
On Wed, 11 Dec 1996 12:21:26 -0500 Microbill-at-aol.com wrote:
} The phosphor plates have (potentially) a higher resolution than currently } affordabel CCD devices. Actually, at the last price (I haven't seen the } latest plate prices) I saw for the plate system you could probably afford a } 2kx2k CCD camera.
TIA, Glen MacDonald Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, Wa 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
~~~~~~~~~~~~~~~~~~~~~~~~ "The box said "Requires Windows 95, or better" so I bought a Macintosh." ~~~~~~~~~~~~~~~~~~~~~~~~
(Please ignore my spelling typo's, it was late last night.) I meant to mention that the Nitrilite gloves are lightweight surgical type gloves, and very easy to work in.
Darrell Miles darrell_miles-at-vnet.ibm.com
IBM Analytical and Test Services Solutions for Industry http://www.chips.ibm.com/services/asg/
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I sent a message out on October 23 saying that Los Alamos would "shortly" be advertising an Electron Microscopy Staff Position in the Center for Materials Science. That position is now finally official and can be found at
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O.k., kept under normal lab environmetal conditions (or reasonable special conditions), i.e. "on the shelf", is there a recognized "shelf life" for Santovac 5? Recognizing the abuse it receives in a diff. pump I would think that its pretty damned stabile, but would like a confirmation of my industrial chemistry ignorance.
Richard E. Edelmann Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513-529-5712 Fax: 513-529-4243 E-mail: edelmare-at-muohio.edu
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Dear Microscopists,
I have to measure the grain size and shape of silver halide particles of normal and high speed b/w films. Are there any tips and tricks on how to remove the gelatine and expose the grains without disturbing their size/shape?
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Dear List Members,
There has been a great deal of experience and opinion discussed recently on the brands of colloidal gold. It seems to me that the conclusion from all this is that there are a number of companies that are making/marketing good products, as none of the comments here have mentioned bad experiences with any. We have found that different products have different shelf lives and differing size stringencies; sometimes these variations are WITHIN THE SAME BRAND. Varying size ranges can be a problem if you're trying to do multiple labelings. Short shelf life can be circumvented by ordering small amounts (0.25 ml). The one brand I endorsed previously always gives us good results, and in most cases, the products last WAY beyond the expiration date. One course would be to find one you like and stick with it to avoid having to test reactivity on myriad products. However, do carry control positive and negative samples in each experiment.
There are numerous companies that distribute these products, some of which I mentioned previously. It appears that, in giving examples of a few sellers, but not all, I have offended some by omission. Perhaps I should have listed all the products on the market or none. At any rate, I meant no offense in my deletions; my sincere apologies. To make amends, I have tried to collect the names of the companies that I'm aware of that sell immunogold commercially (The list may not be complete; if you know of others, please feel free to add them). In no order of preference--only alphabetical:
Amersham 2636 Clearbrook Dr. Arlington Heights, IL 60005 800 323 9750
BB International - Goldmark Biologicals 437 Lock St. Phillipsburg, NJ 08865 908 859 2631
BioRad Laboratories/Life science Group 200 Alfred Nobel Dr. Hercules, CA 94547 510 741 1000
BioRad - Microscience Div. 19 Blackstone St. Cambridge, MA 02139 800 444 1422
Chemicon International, Inc. 28835 Single Oak Dr. Temecula, CA 92590 800 437 7500
Electron Microscopy Sciences 321 Morris Rd Box 251 Fort Washington, PA 19034 800 523 5874
Energy Beam Sciences, Inc. PO Box 468 11 Bowles Rd. Agawam, Mass 01001 800 992 9037
Ernest F. Fullam, Inc. 900 Albany Shaker Rd. Latham, NY 12110 518 785 5533
EY Laboratories 107 N. Amphlett Blvd. San Mateo, CA 94901 800 821 0044
Jackson Immunoresearch Laboratories, Inc. PO Box 9 872 W. Baltimore Pke. West Grove, Pa 19390 800 367 5296
Nanoprobes 25 East Loop Dr., #124 Stony Brook, NY 11790-3350 516 444 8815
Pierce Life Science & Analytical Research Products 3747 N. Meridian RD. PO Box 117 Rockford, IL 61105 800 874 3723
Polysciences, Inc. 400 Valley Rd. Warrington, PA 18976 800 523 2572
Sigma Chemical Co. PO Box 14508 St. Louis, MO 63178 800 325 3010
SPI Supplies PO Box 656 569 E. Gray St. West Chester, PA 19381-0656 800 2424 SPI
Ted Pella, Inc. 4595 Mountain Lakes Blvd. Redding, CA 96003 800 237 3526
And of course, one invaluable (well worth the $75) source of information on antibodies in general is: Linscott's Directory PO Box 26221 Birmingham, AL 35260 800 766 7000
I am not associated in any way with these companies, and I make no warranties on the accuracy of this information or the quality of the products. If you sell gold and are not listed (sorry), feel free to add your name to the hat. Sara
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
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Dear Lucille,
} What are the advantages/disadvantages (including price) in using the } reusable TEM phosphor plates vs. a dedicated CCD camera system for } collecting images? } One disadvantage of image plates vs CCD is that the IP's are not nearly as sensitive at high voltage. The quantum efficiency drops to ~20% at 1250 kV--there was a poster at MSA which had that info. This may be of no interest to you unless you have an HVEM, as I do. Yours, Bill Tivol
The Core Electron Microscopy Facility at Dana Farber Cancer Institute located in Boston, Massachusetts has a position immediately open for a full time EM Technician to assist in daily operation of the central research facility. Requirements include: B.S. or equivalent in life sciences; one to two years experience working as an EM technician; a strong background in transmission and scanning electron microscopy and associated preparative techniques, including tissue processing, Epoxy resin embedding, ultrathin sectioning and contrast staining, immunogold staining, and dark room procedure. Computer knowledge including word processing and data base programs is required. Cryoultramicrotomy skill is desirable but not required. Office management skills including filing, ordering and billing are also helpful. M-F, 40h/W, Salary $20-25K/year with excellent benefit. DFCI is an equal opportunity Employer. If interested, please send by email resume to Yuhui Xu, MD,PhD, at the following address: Yuhui_Xu-at-dfci.harvard.edu. No phone inquiries please.
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I have made three attempts to process human cadaver skin for TEM with no success. Each time, the stratum corneum is separated from the rest of the epidermis, sometimes in sheets.
I have fixed in glutaraldehyde or paraformaldehyde, dehydrated in acetone or ethanol, and embedded in LR White or Spurr's. So far I have not even made it to the TEM because the separation is so evident on my LM slides.
Any suggestions?? Your help is appreciated,
Karen Zaruba
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Life Sciences Sector Lab Reply: kszaruba-at-mmm.com 3M Company 3M Center 270-1S-01 Phone: 612-737-2971 St. Paul, MN 55144-1000 Fax: 736-1519
These opinions are my own and may not represent those of 3M.
Message-Id: {199612140350.WAA25577-at-ns1.axs2000.net} To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Richard Edelman wrote: ========================================= O.k., kept under normal lab environmetal conditions (or reasonable special conditions), i.e. "on the shelf", is there a recognized "shelf life" for Santovac 5? =========================================
Over the years, I have had the chance to visit customers and distributors in tropical kinds of settings, some of which have had Santovac 5 (tm) sitting on the shelf, in unopened bottles, and in non-airconditioned environments for years. While I can recall seeing some of the labels starting to come off, never ever have I ever heard from anyone of an instance of "old" Santovac 5 being inferior to "new" Santovac 5. I am unaware of any kind of guarantee from the manufacturer that would indeed be the case, but from my perspective, there are few things in our laboratories as stable long term as Santovac 5 sitting on a shelf.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ =====================================================
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We are stimulating the grow of liver cells (hepatocytes) in vivo with hepatotrofic factors and we would like to know if the ploidia is being affected. That is, we would like to know if the cells are diploid, triploid or tetraploid by quantifying the DNA in nuclei of cells in histologic sections. Somebody has told me that there is a programa named CAS-200 (DNA QUANTITATION SOFTWARE) that may differentiate between diploid, triploid and tetraploid cells.
Does anyone know how the program works? The same kind of measurements may be done with another program like BioScan Optimas, or SigmaScan?
I would be very grateful for any help ============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 19 5618606 r. 229 Departamento de Ciencias Basicas/Histologia| 55 19 5618887 Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 19 5611689 CEP 13630-000 Pirassununga (Sao Paulo) | BRAZIL | ==============================================================================
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Does any one have any experience in running up the mast cells for Immunocytochem or In Situ at EM level? Since I cannot osmicate the tissue(so far ran up skin, ear lobe or tongue from an albino mice) the subsequent steps of dehydration extracts the granules and makes it difficult to identify the cells. Also the holes make it impossible to do insitu with. Any help and suggestions will be highly appreciated. Thanks in advance. and Seasons Greetings... N. Shah
visit us at... http://www.med.upenn.edu/~path/core/EMCMAIN1.HTM
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} I'm thinking of buying a new low power ( {50x) zoom OM for general } inspection work prior to SEM work. } } If you were going to buy a scope today, what would you get? What would } you avoid? What accessories would you get (i.e. ring lights, diffuse } lighting, polarzing filters, etc.)? } } I'd like only user input, no sales pitches. } Thanks } ------------------------------------------------- } Harold J. Crossman
Harold, Personally, I'd look at Olympus and Nikon first. I did notice at the Society meeting in Mpls that Zeiss has an inexpensive new stereoscope (which they credited to a new automated factory). This one has a clever way of giving dark-field without popping an extra $2000 or so for a special stage. The Russian LOMO would be worth a look, but I've heard varying things about the quality. Accessories will depend on use (such as the dark field I mentioned)--what do you need to do? For polarizing filters, I'd try for some way to mount camera filters, the ones that fit on 35mm SLRs. Lighting will be most critical. Get a multihead fiber optic--2 heads at least--if you can get 2 2-head units. A fiber optic ring light will be useful if you need shadowless illumination. Cameras: get a photo tube even if you don't plan on taking pictures, you'll want to eventually. Don't spend money on fancy photomicroscopy cameras, meters, etc. I've found that an used Olympus OM4 back works fine, and it's metering gives excellent exposures (just make sure the meter is working right). Phil
&&& Illigitimi non carborundum &&&&&&&& Philip Oshel Microscopy Station A PO Box 5037 Champaign, IL 61825-5037 (217)244-3145 days (217)355-3145 evenings oshel-at-ux1.cso.uiuc.edu *** looking for a job again ******************
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Doug
The last few posting that the Microscopy Listserver has from you have consistently occurred twice. Are you intentionally sending 2 copies? I'm trying to sort out things at this end and need to see if it is a system problem or something that you are inadvertently doing.
Thanks for your help
Nestor Microscopy SysOP
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The survey that I sent out had no systematic clues as to why some of you received messageless banners and some did not. There was no real consistent pattern of OS, Email Program or whatever. Except that most of you didn't really know what your Host Email system was running.
Anyway I've not gotten any NEW reports of messages only containing banners for the last day. (BTW this message should have NO BANNER).
There have been a number of "Return Receipt" messages/reports. These occur when one of two thing happens.
First, you have checked an option in your Email program which embeds a hidden command in your Email message to notify the you when the person you have sent the message reads, deletes, or receives the message. PLEASE check you system. If you have such an option TURN IT OFF. The Listserver becomes the owner of all messages to the system, hence return receipts loop right back to the system. Those of you who have been on this system long enough will remember the disaster we had about a year ago when a message setup with automatic reply created an infinite loop.
Secondly, some programs (very few) also have options which all them to inform the sender that a message has been received, deleted, read etc... If you have' that option also please turn it off.
I have send message to those individuals that I can identify whose computers have started posting the problem messages and have temporairly removed them from the Master Database. When they fix their Email Program then I will reinstate them.
Hopefully this will cure the latest set of problems that have cropped up.
Please continue to report to me any problems with mail dated from Monday 12/16/96.
Do Not bother to report problems with messages dated before then as I will already have those on record.
If you are still receiving messageless banners on 12/16 please also report it directly to me.
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I chose to get a Leica M420 Macroscope for this precise purpose. We Had a good stereo microscope (Wild) which I used for years. Good optics for stereo viewing but when we wanted images the off axis nature of the images was very obvious. The Macroscope works in the same magnification range but with on-axis optics the recorded images are superior. I bought the achromat objective but there is a apochromat available for top performance. I did not buy the photo system but put a CCD B/W video camera on a C mount on the macroscope.
No connection with Leitz, just happy with my choice.
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Season's Greetings everyone,
This is a question for users of the DS-130 model of SEM made by ISI (an oldie but goodie).
Does anyone know how to use the "Objective Lens" and "Scan 2" controls to get very low magnification, low distortion images? The manual implies that you simply turn both of these controls to the "off" position, but if I do that I get a very small field of view (because of the objective aperture silhouette) and an image which cannot be focused. It is quite low mag though. Perhaps I should mention that I use the obj aperture intended for the first stage in second stage mode also, because the image is much brighter that way (i.e. the aperture at the bottom of the column has been removed). Even if that is the cause of my aperture silhouette problem, I am still left unable to focus. Any ideas would be welcomed...
Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department School of Medicine University of Auckland Private Bag 92019 Auckland New Zealand
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Greetings, Anyone out there know of good software for fitting splines to data? At present, we are struggling with SAS, which works, but from which it is tricky (!) to extract the relevant coefficients. Note, we need to fit a smooth curve through data for which we do not have a model function, hence our interest in splines. We don't just want to drive a spline curve through each data point, which is all that a couple of programs we have checked can do. I realize that this is not explitly a biophysics or microscopy question, but as the our data have been generated from image analysis through a microscope, I hope I may be forgiven for supposing someone on these lists could help. Thanks, Tobias Baskin
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Seasons greetings to everyone.
I wonder if anyone knows a source, person or organisation, that would have a NiSi EDS standard for the SEM in the composition range 10- 50at%Ni (about 20-75wt% Ni) - a sample in the range 10-20 at%Ni would be great.
Mike
Michael J Witcomb PhD Electron Microscope Unit University of the Witwatersrand Private Bag 3 WITS 2050 South Africa
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Hello,
It may be necessary to open the HV tank on our JEOL 4000. In doing so we will have to replace the sodium hexafluoride insulation gas (no, not oil) that resides there. Have any of you with this type of tank had to buy SF6 lately? Can anyone recommend a distributor that currently has this product in stock?
FYI, SF6 is about $4,000 per standard cylinder and hard to locate. Also, it is a consumable and therefore not covered by service contract!!!
There goes the new image analysis software package....
Dear Santa, all I want for Christmas is some SF6....
Happy holidays, Owen Owen P. Mills Michigan Technological University Metallurgical & Materials Engineering Rm 512 MME Building Houghton, MI 49931 906-487-2002 906-487-2934 FAX opmills-at-mtu.edu
To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com} Cc:
To: {microscopy-at-Sparc5.Microscopy.Com} Cc:
To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com} Cc:
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Dear microscopists,
this week we've had problems calibrating our EDAX PV9100, ECON II, system attached to our SEM.
We usually use a Cu-Al stub for calibrating at two peaks: Al Ka and Cu ka. We situate the sample in such a way that we see on the screen half area Al and half area Cu in order to get both peaks with about the same height.
This week we had the following problem: we calibrated like always but, this time the Cu Ka shifted on the energy axis to about 3.3 keV. Of course, the Al peak was totally missed. Such a shift we had never seen.
By adjusting the Zero, the Gain and the Fast discrimator settings at the preamplifier we could, after trying for a afternoon, register the Al peak and calibrate both peaks at the right energy.
The problems that remains unsolved is the height of the peaks. As we get the equal areas (Cu and Al) on the screen, we now get a Cu peak which is almost 25% of the height of the Al one. What does it mean? Are we getting some of the Cu signals cut by the preamplifier ?
Our electronic technicians have no idea about it's going on. Does anyone have any suggestions about what the problem is? If so, could you please comment them to us? We would appreciate it very much.
Thanks in advance. Silvia Montoro Centro Regional de Investigacion y Desarrollo de Santa Fe Santa Fe - Argentina csedax-at-arcride.edu.ar
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Owen Mills asked about SF6 gas cylinders.
We get ours through AGA. Large cylinders cost us about $500, not $4000! Try calling AGA's main office in Ohio for a local distributer: (419) 893-7226.
Russell E. Cook Electron Microscopy Center for Materials Research Argonne National Laboratory 9700 South Cass Avenue MSD-212 Argonne, IL 60439 (630)252-7194 FAX: (630)252-4798
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Has anyone been looking at the scattering from colloidal gold particles as positional tags on proteins?
I am interested in tagging enzymes and tracking their location in a conventional research microscope by tagging them with ~1-5 nm gold particles. I understand from second-hand sources, and from a few abstracts I have seen (but have not yet looked at the articles), that ~4 nm gold particles may be seen just from their scattering properties in phase contrast or DIC mode.
I have one article (J of Microscopy, Vol 173, Pt 1, Jan 1994, pp27-38) that demonstrates that 1 nm gold particles may be viewed in reflection mode using a confocal microscope. Does anyone know what size gold particle might be viewed in a conventional microscope in perhaps dark field reflection mode?
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Hello,
We work on human skin. Is the cadaver skin fixed in formaldehyde before you get it? If so, the ultrastructure will suffer. An excellent fixtive to use is half strength karnovsky and embed in epon. LR White has the strange problem of not polymerizing where the tissue is filaggrin rich, which is right at the transition cell layer, thus the stratum corneum has trouble staying on. Bob Morphology Core U of Washington
On Fri, 13 Dec 1996, Karen S. Zaruba wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } I have made three attempts to process human cadaver skin for TEM with no } success. Each time, the stratum corneum is separated from the rest of the } epidermis, sometimes in sheets. } } I have fixed in glutaraldehyde or paraformaldehyde, dehydrated in acetone or } ethanol, and embedded in LR White or Spurr's. So far I have not even made it } to the TEM because the separation is so evident on my LM slides. } } Any suggestions?? Your help is appreciated, } } Karen Zaruba } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Life Sciences Sector Lab Reply: kszaruba-at-mmm.com } 3M Company } 3M Center 270-1S-01 Phone: 612-737-2971 } St. Paul, MN 55144-1000 Fax: 736-1519 } } These opinions are my own and may not represent those of 3M. } } } }
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Dear Karen,
Here is a trick which has worked for retina. Try embedding the skin in gelatin first, even before it is fixed. This should help to hold the layers together during subsequent manipulations and further processing.
If fixation is done before you receive the tissue, the gelatin may still prevent your sample from separating as much. Good luck and keep us posted.
Lisa Hartnell, Res. Associate Yale University School of Medicine Center for Cell Imaging Hartnell-at-biomed.med.yale.edu
On Fri, 13 Dec 1996, Karen S. Zaruba wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } I have made three attempts to process human cadaver skin for TEM with no } success. Each time, the stratum corneum is separated from the rest of the } epidermis, sometimes in sheets. } } I have fixed in glutaraldehyde or paraformaldehyde, dehydrated in acetone or } ethanol, and embedded in LR White or Spurr's. So far I have not even made it } to the TEM because the separation is so evident on my LM slides. } } Any suggestions?? Your help is appreciated, } } Karen Zaruba } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Life Sciences Sector Lab Reply: kszaruba-at-mmm.com } 3M Company } 3M Center 270-1S-01 Phone: 612-737-2971 } St. Paul, MN 55144-1000 Fax: 736-1519 } } These opinions are my own and may not represent those of 3M. } } } }
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Hi Everyone;
I have a general question that may be obvious to some, but is not obvious to me. When is a sputter coater target "spent", and require replacement? Does it simply no longer coat the sample effectively, or is it something more subtle? In the case of a blended target (mine is 60:40 Au/Pd), does one element deplete before the other? I have had the same target for several years now.
TIA,
-Bob ******************************* Bob Citron Chiron Vision 555 W. Arrow Hwy Claremont, CA 91711 USA Ph: (909)399-1311 Email: Bob_Citron-at-cc.chiron.com *******************************
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Westinghouse Science and Technology Center Pittsburgh Pa has an opening for a physical metallurgist/electron microscopist to provide TEM/AEM support for the metallurgy group. The materials to be studied are mainly nuclear and fossil based power generation materials. For more details contact : Mike Burke Manager Metallurgy Section Westinghouse Science and Technology Center Pittsburgh Pa
One thing that can cause relative peak heights to vary is the electron accelerating voltage being used. If you normally have been working around 20 kV and then drop down to a relatively low operating kV, say 12 kV, you may get relatively inefficient excitation of the Cu atoms (because Eo {2Ec), whereas the Al atoms will be efficiently excited (because Eo} 5Ec) and this can cause the Al peak to be smaller than the Cu peak (the Cu L peak will also be larger than the CuK peak). The old 'rule of thumb' was to choose an accelerating voltage that is 2 to 3 times the critical excitation voltage of the highest energy line being used in the analysis.
Sorry to send this again, but I made a typo in the first version and typed 'smaller' instead of 'LARGER": see below ;
One thing that can cause relative peak heights to vary is the electron accelerating voltage being used. If you normally have been working around 20 kV and then drop down to a relatively low operating kV, say 12 kV, you may get relatively inefficient excitation of the Cu atoms (because Eo {2Ec), whereas the Al atoms will be efficiently excited (because Eo} 5Ec) and this can cause the Al peak to be LARGER than the Cu peak (the Cu L peak will also be larger than the CuK peak). The old 'rule of thumb' was to choose an accelerating voltage that is 2 to 3 times the critical excitation voltage of the highest energy line being used in the analysis.
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} Anyone out there know of good software for fitting splines to } data? At present, we are struggling with SAS, which works, but from } which } it is tricky (!) to extract the relevant coefficients.
Sounds like a trick for either MathCad {http://www.mathsoft.com} or Mathematica {http://www.wolfram.com} , although Igor Pro might be a good choice {http://www.wavemetrics.com} . I personally Prefer MathCad for my data analysis, because equations are written and executed just like you would expect (i.e. as you would write them out on paper). Good luck!
-Kirk ___________________________________________________ No Window of Opportunity: Upgrading from Windows 3.1 to Windows 95 or NT is not as cheap as some might have thought. The Gartner Group Inc., found that it takes two to three days per desktop to do the conversion. This translates into thousands of dollars and countless new headaches.
- Client/Server Computing, August '96 ___________________________________________________ This message was created and sent using the Cyberdog Mail System ___________________________________________________ Kirk Rogers E: rogers-at-er6.eng.ohio-state.edu Office: (614) 688-4067 FAX: (614) 292-1537 Materials Science and Engineering The Ohio State University 477 Watts Hall, 2041 College Ave. Columbus, OH 43210
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} Anyone out there know of good software for fitting splines to } data? At present, we are struggling with SAS, which works, but from } which } it is tricky (!) to extract the relevant coefficients.
Sounds like a trick for either MathCad {http://www.mathsoft.com} or Mathematica {http://www.wolfram.com} , although Igor Pro might be a good choice {http://www.wavemetrics.com} . I personally Prefer MathCad for my data analysis, because equations are written and executed just like you would expect (i.e. as you would write them out on paper). Good luck!
-Kirk ___________________________________________________ No Window of Opportunity: Upgrading from Windows 3.1 to Windows 95 or NT is not as cheap as some might have thought. The Gartner Group Inc., found that it takes two to three days per desktop to do the conversion. This translates into thousands of dollars and countless new headaches.
- Client/Server Computing, August '96 ___________________________________________________ This message was created and sent using the Cyberdog Mail System ___________________________________________________ Kirk Rogers E: rogers-at-er6.eng.ohio-state.edu Office: (614) 688-4067 FAX: (614) 292-1537 Materials Science and Engineering The Ohio State University 477 Watts Hall, 2041 College Ave. Columbus, OH 43210
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Back a while ago, I acquired a few low-mag images for some of our std discs. I believe it is the PROBE-CURRENT knobs can be used for focusing the low mag image (may called SPOT-SIZE, CONDENSER, or RESOLUTION on yours?). This is done under electron chenneling pattern observation. Hope it helps.
Xiaogang
*********************************** * Xiaogang Xie * * SEM & Microprobe lab * * Dept. of Geology and Geophysics * * Louisiana State University * * Baton Rouge, LA 70803 * * Office (504)388-2240 * * Fax (504)388-2302 * ***********************************
We get our SF6 from Scott Specialty Gases in NJ, but they might have offices in your area. Their number is (908)754-7700. We use it in our JEM2010 and we order VLSI grade. I believe it is also called instrument grade (99.995 purity).
Jordi Marti AlliedSignal ----------
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Hello,
It may be necessary to open the HV tank on our JEOL 4000. In doing so we will have to replace the sodium hexafluoride insulation gas (no, not oil) that resides there. Have any of you with this type of tank had to buy SF6 lately? Can anyone recommend a distributor that currently has this product in stock?
FYI, SF6 is about $4,000 per standard cylinder and hard to locate. Also, it is a consumable and therefore not covered by service contract!!!
There goes the new image analysis software package....
Dear Santa, all I want for Christmas is some SF6....
Happy holidays, Owen Owen P. Mills Michigan Technological University Metallurgical & Materials Engineering Rm 512 MME Building Houghton, MI 49931 906-487-2002 906-487-2934 FAX opmills-at-mtu.edu
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In a message dated 96-12-16 19:01:35 EST, Bob_Citron-at-cc.chiron.com (Bob Citron) writes:
} When is a sputter coater target "spent", } and require replacement? Does it simply no longer coat the } sample effectively, or is it something more subtle? In the case of a } blended target (mine is 60:40 Au/Pd), does one element deplete before } the other?
Typically (at least for the small foil types used in small EM-type coaters), it's spent when you sputter a hole in it. Take it out of the holder and hold it up to the light- if you can see little pinholes, it's time. If you leave it too long, you'll start sputtering the face of the cathode.
If the target is bonded, you'll start to sputter the substrate when it burns through, this may be harder to detect by visually examining the target. I have no experience w/ bonded targets.
As for a blended target, the ratio shouldn't change as it is used.
Message-Id: {2.2.32.19961217050339.00688834-at-pop.unixg.ubc.ca} X-Sender: mager-at-pop.unixg.ubc.ca X-Mailer: Windows Eudora Pro Version 2.2 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
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Dear Silvia Montoro: You wrote: ... } This week we had the following problem: we calibrated like always but, this } time the Cu Ka shifted on the energy axis to about 3.3 keV. Of course, the Al } peak was totally missed. Such a shift we had never seen. } The only time I had such a drastic shift in peaks was when my Kevex 8000 got a broken wire in the high-voltage bias supply. The broken wire was under the heat-shrink behind the BNC connector, so it couldn't be seen. The bias degrades slowly, so the peaks were gradually going lower and broader. This bias is difficult to test accurately because it is high voltage but almost no current, so check your manuals carefully. Luck, Mary Mary Mager Electron Microscopist Metals and Materials Eng., UBC 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel:604-822-5648, fax:604-822-3619 e-mail: mager-at-unixg.ubc.ca
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Dear Bob:
It is my understanding that gold and palladium sputter congruently unlike evaporation where the two metals are deposited as a function of their melting point. You could check this by depositing some Au-Pd and checking the ratio br XRMA. We consider a target is worn out when holes begin to appear. We don't seem to get much joy in re-cycling target material.
Seasons greetings from Cambridge UK
Patrick Echlin Multi-Imaging Centre Scool of Biological Sciences.
On Mon, 16 Dec 1996, Bob Citron wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } Hi Everyone; } } I have a general question that may be obvious to some, but is } not obvious to me. When is a sputter coater target "spent", } and require replacement? Does it simply no longer coat the } sample effectively, or is it something more subtle? In the case of a } blended target (mine is 60:40 Au/Pd), does one element deplete before } the other? I have had the same target for several years now. } } TIA, } } -Bob } ******************************* } Bob Citron } Chiron Vision } 555 W. Arrow Hwy } Claremont, CA 91711 } USA } Ph: (909)399-1311 } Email: Bob_Citron-at-cc.chiron.com } ******************************* }
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On Fri, 13 Dec 1996, Kris Kovacs wrote: } } Dear Microscopists, } } I have to measure the grain size and shape of silver halide particles of } normal and high speed b/w films. Are there any tips and tricks on how to } remove the gelatine and expose the grains without disturbing their size/shape? } } Thanks, } } Kris
Dear Kris,
You can release silver halide particles from b/w films by an enzymatic hydrolysis of gelatin in a saturated solution of Pronasa E for 45-60 min at t=38-40oC following by weak centrifugation (2,000 rpm) for 10 min. It is necessary to resuspend the deposit several times and to wash thoroughly with distilled water following by the centrifugation to remove gelatin. Separated grains may be coated on thin films (TEM) or on slides (SEM). In order to avoid silver halide decomposition under the beam, one can use cooling at LN2 temperature and/or one-step carbon replicas.
For the production of replicas a carbon film should be evaporated onto particles deposited on glass slides in a vacuum unit at about 10-3 Pa. The carbon film may be separated from the support by a treatment with 2-5 % aqueous HF solution following washing with distilled water to remove traces of acid, dissolving the grains in a 10% sodium thiosulfate solution and washing again with distilled water. Afterwards, the film should be coated on the grids and dried.
Sometimes, b/w films, in particular, high speed ones may have a composite arrangement which comprises high-sensitive and low-sensitive semilayers with emulsions of different sizes and shapes. Therefore, ultramicrotomy (TEM) and cryofractography (SEM) of films is also useful and grains may be released from different semilayers using a layer-by-layer hydrolysis of gelatin by the choose of an appropriate time of the treatment. Some references and examples of such studies you can find in our review published in Microbeam Analysis, 4, (1995), 1-29.
Regards,
---------------------------------------- Vladimir Oleshko, Ph.D. University of Antwerp (UIA) Chemistry Department Micro- and Trace Analysis Centre (MiTAC) Universiteitsplein, 1 B-2610 Antwerpen-Wilrijk, Belgium Tel.: +32-3-820.23.64 Fax: +32-3-820.23.76 e-mail: oleshko-at-uia.ua.ac.be ----------------------------------------
To: Microscopy-at-Sparc5.Microscopy.Com (Microscopy Listserver U.S.A.)
Hello,
I have noticed, that the discussion about SF6 gas opened on Listserv. I have another question. I heard that it is possible to replace Freon gas in TEM Jeol 2000FX by SF6 gas without changing the electron gun (SF6 needs higher pressure than Freon), only some attachment is necessary for joining the SF6 tank to microscope Freon system. Does anybody know about supplier of SF6 gas in Europe, who can provide this service in Slovak Republic?
Thank you
Yours sincerely
Peter Makroczy makroczy-at-ccsun.tuke.sk ---- __________________________________________________________ Peter MAKROCZY Technical University of Kosice Department of Materials Science Park Komenskeho 11 040 01 Kosice SLOVAK REPUBLIC
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POST-DOCTORAL POSITION: "Analytical Electron Microscopy of Oxynitride Glass Ceramics"
Division for Microscopy and Microanalysis Chalmers University of Technology S-412 96 G=F6teborg SWEDEN
A post-doctoral position is available within the framework of the= Training and Mobility of Researchers (TMR) Programme. Qualified candidates= will have a Ph.D. in materials science, chemistry of physics as well as documented experience with analytical electron microscopy. The candidate must be a national of a Member State of the European Community.
The research project, "Structure and Properties of New M-Si-Al-O-N Oxynitride Glass Ceramics (M =3D Y, Ln)", is a collaboration between= seven partners in the United Kingdom, Ireland, France, Sweden and Belgium.= The aim of the project is to determine structures and properties of currently uncharacterized glass-ceramics in yttrium and rare earth sialon systems.= =20 These materials are potential refractory grain boundary phases in Si3N4-based ceramics, and have an interst also as refractory surface coatings (glazes) and as interface materials in nitride-oxide and nitride-metal joints.
The work carried out at Chalmers will focus on the chemistry and= structure of glasses and their crystallisation products, and on microstructural development during nucleation and growth processes. The major part= of the microscopy will be carried out on a Philips CM200 SuperTwin TEM equipped with a field emission gun (FEG), the Gatan imaging filter (GIF) and= the Link Isis EDX system.
The post-doctoral researcher will initially be appointed for one= year with a possibility to prolong the appointment. The maximum duration is= 2.5 years, and the project has to be finished by the end of December= 1999. The starting date of the appointment can be discussed, and the starting= salary is around 3.394 ECU per month.
The collaboration within the established network will give the post-doctoral researcher good contacts with a number of research= groups in Europe and a good knowledge of a variety of preparative / analytical= / property measurement techniques relevant to the evaluation of new= ceramics as well as other materials. The work will involve visits to the= other partners in the network.
=46or futher details, please contact:
Dr. Lena K.L. Falk, Division for Microscopy and Microanalysis, Department of= Physics, Chalmers University of Technology, S-412 96 G=F6teborg, SWEDEN. = =20 = =20 e-mail: lklfalk-at-fy.chalmers.se; fax: + 46 31 772 3224;= =20 phone: + 46 31 772 3321
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In the past there have been a number of interesting threads on this listserver regarding various health issues of importance to electron microscopists (e.g. do SEM's cause cataracts, etc.). While I'm generally not a fanatic about these sorts of items and tend not to worry about them, I thought I should bring up an interesting conversation I had in a bar the other night. I was talking to this guy, who, when he found out I run an SEM lab, told me that for several years he had worked in an office with two other gentlemen which had been situated directly behind an SEM lab. This would have been more or less from about 1980 to 1990, give or take a year. Now for the interesting part. He told me that so far the other two fellows have had, between them, three hip replacement operations, with a fourth pending, and that my informant was also beginning to have problems with his hips, and had already found that his athletic activities were beginning to be curtailed. ( All three of the guys in question, I might add, are stated to be now just in their late forties or fifties.) He had, of course, become concerned and had been looking for some common factor in their work environment which may have affected all three of them, and the only thing he could come up with was the proximity of their workstations to the SEM on the other side of the wall. Three out of three is, after all, an impressive statistic, even in a small sample, when you're one of them. My informant had been informally researching SEM's in the past few weeks, and was aware that X-ray emissions are generally not an issue with them, but was becoming increasingly interested in possible electromagnetic fields associated with these instruments. He told me of research which tends to suggest that longterm exposure to EM fields may have serious effects on bone joints, so if SEM's do indeed create strong local EM fields, there may be some connection. So, I wonder.... Do you suppose that there may be anything to this? Do people with longterm associations with SEM labs exhibit higher than average incidences of bone/joint disorders? For that matter, do SEMs create EM fields capable of affecting people, say, ten feet away on the other side of a wall? Now I don't generally jump on every health scare bandwagon that comes along, but this fellow appeared to be a reasonably well-educated and intelligent guy (and never once mentioned aliens) so I thought I'd throw his story out to the listserver and see what comes of it. And Season's Greetings, by the way. F.C. Thomas GSC (Atlantic) MicroAnalysis Facility P.O. Box 1006, Dartmouth, N.S. B2Y 4A2 (902) 426-4635
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A failure of the pulse processor (typically, the ADC) can also cause apparent shifts in peak energies. The frist thing (and easiest) to check are the power supply volatges and especially the ADC reference voltages. _Woody_
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Frank Thomas recently raised the question regarding EM fields associated with SEMs and health.
My direct experience with electron optical instruments goes back to 1970 and lasted 23 years. This included a daily dose of whatever fields were associated with an EPMA and SEM. During this period I have suffered no health problems other than the usual occupational hazards of working in the dark, i.e., fungus and moss and the distinct personality of a troll. The only adverse affects I am aware that electron microscopists have are related to the ergonomics of operating the instruments, i.e., repetitive motion syndrome, back strain, eye strain, etc.
In my opinion the hip deterioration described raises more questions about the quality of the drinking water that any field environment. I would assume the average circuit breaker panel would have a higher electromagnetic field than an SEM.
Looking forward to the opinions and experiences of others.
We did a survey for EM fields in my TEM (300kV) lab (HRSEM in the next lab). The highest field in the room was associated with my color monitor for the EDX system. Second highest source was the fluorescent lights.
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Hi Bob & fellow microscopists!
Let me start by introducing myself, I am the Business manager for the POLARON range of preparation equipment including coaters. Your question to the target dilemma is simple, but interesting one.
The most simple answer is that when the target becomes perforated then it is deemed to be spent. BUT, depending on the backing metal it is possible to continue sputtering with this same target for some time.
What happens is that the target area that is being bombarded is reduced so the sputter rate is continually in decline, if the coater has a sufficiently strong Plasma then the backing metal will be sputtered at a slower rate but will contaminate the gold or target material. Not a problem for visualisation of the surface by SE detector.
When the target is of a mixed metal composition then the softer (Less dense) material will erode quicker, in practice this is not significant as the mixed metal is used to stop conglomeration and island formation of the sputtered material (normally gold) hence offering higher resolution or more correctly, smaller grain size.
Our recommendation for reproducible high quality work is to change the target once perforated.
There is much more that we could discuss but I hope this answers your question.
Best regards - Tony
Regards,
Tony King Product specialist VG Microtech/ Polaron range
Tel: +44 (0)1825 746251 Fax: +44 (0)1825 768343
E&OE ******************************** Energy Beam Sciences, Inc. Adding Brilliance To Your Vision ebs-at-ebsciences.com http://www.ebsciences.com/ ********************************
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Has anybody a good technique (or reference) to stain for sucrose in moss-tissue, pressure-frozen, freeze substituted and Lowicryl embedded thin sections? Please? My email address is A.M.Schmid-at-qmw.ac.uk
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I'm interested in pricing a chamber scope (scope and monitor and whatever accessories are needed) for a Hitachi 2460N. Does anyone out there in the yelm of microscopy have any recommendations? Please respond directly to me and not the list.
Thanks,
Bob
Robert R. Wise, PhD Director, UWO Electron Microscope Facility Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (414) 424-3404 tel (414) 424-1101 fax wise-at-uwosh.edu
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To all,
Portions of this subject have been discussed over the past few months but I haven't really been paying close attention to it. I apologize in advance if we rehash ground already covered. We have a (an?) Hitachi 2460N SEM and I'm looking into outfitting it for digital image capture (with annotation, file storage, printer, etc). I know that Hitachi sells a system that is supposed to plug right in and comes with a lot of bells and whistles. Has anyone had any experience with the Hitachi system? Alternatively, does anyone know of an after market product that performs well? Given that I am a computer dummy, I would be more inclined to purchase a total system rather than attempt to put one together on my own from individual components. Please respond directly to me and not to the list.
Thanks in advance,
Bob
Robert R. Wise, PhD Director, UWO Electron Microscope Facility Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (414) 424-3404 tel (414) 424-1101 fax wise-at-uwosh.edu
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Hello,
A colleague of mine is investigating hourly rates charged for XRD & XRF. I believe many of you may have this instrumentation in or near your EM labs. Will you contact me off line with any info on hourly rates for that type of instrument. Thanks.
Owen Owen P. Mills Michigan Technological University Metallurgical & Materials Engineering Rm 512 MME Building Houghton, MI 49931 906-487-2002 906-487-2934 FAX opmills-at-mtu.edu
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I saw similar symptoms once in a detector on an SEM. We warmed and pumped the detector and it then worked again. I believe we first checked the detector on another analyzer we had available, so we knew the problem was in the detector/preamp area.
Tony.
**************************************************** **************************************************** ** ** ** Anthony J. Garratt-Reed ** ** Room 13-1027 ** ** Center for Materials Science and Engineering ** ** Massachusetts Institute of Technology ** ** 77 Massachusetts Avenue ** ** Cambridge, Massachsetts 02139-4307 ** ** U. S. A. ** ** ** ** Phone: 617-253-4622 ** ** Fax: 617-258-5286 or 617-258-6478 ** ** ** **************************************************** ****************************************************
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Hi Netters, =09Recently, there was a discussion on sectioning metal stents which=20 I didn't save because we don't do them. However, I have just received=20 this message from a former student. Can anyone help him? Thanks. Sara
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710=20 Ph: 919 684-3452 FAX: 919 684-8735
---------- Forwarded message ----------
Dear Dr. Miller,
I'm not sure if you remember me. I'm the biomedical engineering grad student that took your EM class several years ago. I just graduated in September with the PhD. I'm in Munich Germany now doing some post doctoral work on the stenting or coronary arteries.
I'm writing to you to ask if you have any infomation regarding the section of tissue that contain small pieces of metal. The stent that we are researching is made of stainless steel and we would like to cut some type of plastic embedded sections at around 4 =B5m thick, remove the plastic and do some immunohistochemistry. We do have a published report where they do just that, but we have tried to use the polymethylmethacrylate and it produces artifacts for use. It looks like the plastic is not as hard as the steel and the cutting knife (tungsten carbide?) pushed the small steel piece, producing an artifact in the tissue next to the metal.
Do you know of any plastics that are harder than PMMA or anyone I could contact that might be of any assistance. I know this is not much information, but I thought you might be able to help.
Thanks for you assistance, Have a Merry Christmas
Robert Herrmann rah1-at-acpub.duke.edu
Robert Herrmann, Ph.D. Klinikum rechts der Isar Munchen, Germany rah1-at-acpub.duke.edu
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Hi Bob. I maintain an archive of most of the biologically relavant postings to this list. You are correct that this has been a hot topic in the last few months and I have archive it at our web site. Go to the URL listed at the end of this message and click on the "Tips & Tricks" link. Within there you will find a section on "Computers" Look at the section titles "Acquiring Digital Images". Let me know and I can get you the info in some other way if you need. Good Luck
} To all, } } Portions of this subject have been discussed over the past few } months but I haven't really been paying close attention to it. I apologize } in advance if we rehash ground already covered. } We have a (an?) Hitachi 2460N SEM and I'm looking into outfitting } it for digital image capture (with annotation, file storage, printer, etc). } I know that Hitachi sells a system that is supposed to plug right in and } comes with a lot of bells and whistles. Has anyone had any experience with } the Hitachi system? Alternatively, does anyone know of an after market } product that performs well? Given that I am a computer dummy, I would be } more inclined to purchase a total system rather than attempt to put one } together on my own from individual components. } Please respond directly to me and not to the list. } }
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { GO GATORS Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 352-392-1295 ICBR EM Core Lab fax 352-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ The home of " Tips & Tricks "
"To do the honors of a table gracefully, is one of the out-lines of a well-bred man; and to carve well, little as it may seem, is useful twice every day, and the doing of which ill is not only troublesome to ourselves, but renders us disagreeable apt ridiculous to others."
Reverend John Trusler, 1788, quoting Lord Chesterfield.
We just had a survey of the magnetic fields run on our two SEM labs, one with an Hitachi S-800 and the other with an Hitachi S-520, in each case with the instrument in operation. Nowhere in either lab was there a field greater than a fraction of a milligauss (specs for the S-800 require less than 0.5mG). If the SEMs in your labs are performing anywhere near to specifications it is likely that the fields in your labs are of about the same magnitude. The strength of fields of this kind fall off as the third power of distance [i.e. B = f(1/r^3)], and so it seems highly unlikely that any fields produced in the SEM lab would make a measurable contribution in an adjoining office. In fact, it is quite likely that the fluorescent lights in a typical office, or normal ground currents through the structural members of the building, would produce higher fields. Statistically, it is quite possible for highly unlikely events (such as three people having joint trouble) to occur. The frequency with which this happens is usually quite low, however, and so such events are commonly called miracles or catastrophies, depending on the perspective from which we view them. [Have these guys been getting plenty of calcium (1200 mg/day), magnesium (200 mg/day) and vitamin D in their diets?]
Due to the problems either from our network or our computer, I have not received any messages from this listserver for two days. Therefore I will appreciate it very much if anyone of you who responded to my job posting( EM Tech at Dana Farber Cancer Institute) re-send your response to me. Sorry for the inconvenience.
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At 08:16 17/12/1996 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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At 11:02 17/12/1996 GMT, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America a 400 Kv. Concerning the 2000FX or EX it's not so easy just increasing the pressure in gun and what about the HV Tank? And all the safety devices has been set for Freon Gas. So it's necessary to readjust them. Hope the discussion will continue. Greetings. ========================================================== Jacky Larnould tel 33 (0)4 67 72 28 26 fax 33 (0)4 67 79 54 90 email larnould-at-mnet.fr
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I have made many measurements of Magnetic field strength around our SEMs. They do not radiate large fields. They have metal shielding around the columns to keep fields OUT and these and the iron shrouds in the lens casings very effectively keep fields IN. Also the heavy lens currents are DC. The AC current driving the scan coils is realtively small. Any fields that escape from the system fall below ambient at half a metre (say 2 feet) from the column. The rest of the system is also designed so strong fields are not radiated to interfere with the microscope.
There are far stonger sources of ELF AC fields around our building, giving a general ELF AC background of 10-20 milligauss. In fact it is hard these days to locate any area with a field less than 3 milligauss, even in the open air.
I am unconvinced that ELF AC fields are harmful to health. But I am sure that the popular cosmetic appearance of a sun tan is a radiation burn which can lead to skin cancer. Pick your own risk!
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Fellow Microscopists;
Many thanks to all who responded to my questions about sputter targets. I pulled mine off and examined it for pitting (as you suggested). It's 5 years old and still good...
-Bob **************************** Bob Citron Chiron Vision 555 W. Arrow Hwy Claremont, CA 91711 USA (909)399-1311 Bob_Citron-at-cc.chiron.com ****************************
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Hi:
Problem: Need to remove cuticular hydrocarbons from flies and have them alive after the trauma! Any suggestions?
Thanks.
Wish to summarize responses if enough interest. Let me know if OK to acknowledge your replies (as I like to do but, once in a while one stumble across people who wish their remarks not be attached to their id, thus my request for permission).
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At 04:33 PM 12/17/96 -0400, you wrote: } The strength of fields of this kind fall off as the third power of distance } [i.e. B = f(1/r^3)],
I have heard this a few times now, but can someone tell me why it is the 3rd power? I thought the energy flux or intensity fell off as 1/r**2 from a point source. The area over which the energy is spread increases with r**2. For a line source the fall-off was 1/r, while for a plane source there was no fall-off.
Can you physics majors out there explain this to me. I know enough EE to be dangerous. Is it that magnetic field strength is different? ---------------------------------------------------- Warren E. Straszheim 270 Metals Development, Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091
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Dear all
A quick question. We do have Rhodin's Histology Text and Atlas which serves us well. Is there anything similar available for SEM sample identification?
Thanks and Seasons Greetings to all * **** ****** *********** [][]
## [########] ## ## ## ## ## Stephan H Coeztee Electron Microscope Unit Private Bag 3 Wits 2050 South Africa
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Since there is a current discussion on targets, I have become very curious regarding those people who get an incredible 5yrs + out of their targets. Now granted there are differing rates of usage, and differing deposition amounts in various labs and users, but it would seem that target thickness is one of the major factors in this longevity.
I have a Denton Desk II, which takes a disk target (2.375" diameter). Looking through the catalogs and talking with a few other EM labs, targets vary from 0.0015" to 0.01" thick. Since we are generally talking about Au or Au/Pd electrical conductivity/ resistance through the target shouldn't be a major concern (?). Is there any procedures, other than trial and error, for determining how thick a target one can use? Or even more specifically can anyone give me the answer for a Denton Desk II ?
Note: It is not my intent to support nor condem in any way any sputter coater vendor.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513-529-5712 Fax: 513-529-4243 E-mail: edelmare-at-muohio.edu
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Stephan,
Kessel RG, Shih CY, 1973. Scanning Electron Microscopy in Biology: A Student's Atlas on Biological Organization. Springer-Verlag (New York), 345 pp.
Motta P, Andrews PM, Porter KR, 1977. Microanatomy of Cell and Tissue Surfaces: An Atlas of Scanning Electron Microscopy. Lea & Febiger (Philadelphia).
Kent
A. Kent Christensen, University of Michigan {akc-at-umich.edu}
------------------------
On Wed, 18 Dec 1996, Stephan Coetzee wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } Dear all } } A quick question. We do have Rhodin's Histology Text and Atlas } which serves us well. Is there anything similar available for SEM } sample identification?
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I just read an article in a local paper (originally from the LA Times) linking EMF exposure to Alzheimer's incidence. It references an article in today's issue of the journal Neurology, authored by Dr. Eugene Sobel of USC. The most startling statement was "the greatest risk was for people who operate sewing machines". The theory is that the exposure is high because they work so close to the electrical motor in the machine. Another high risk group were carpenters and others who use electrically power tools held close to the body.
I think it helps to show that there is still much debate on the hazards of exposure to EMF fields and that less obvious, mundane activities could pose serious risks.
Louie Kerr
Louie Kerr Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX)
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Yuck! We have DAB precip on our immunocytochemistry sections. They are Araldite embed sections on slides that we have etched and circled with PAP pen. Has anyone else had this problem and do you have any solutions? Is it possible to get the precip off once it has formed? We haven't had any luck. Any suggestions would be greatly appreciated.
Cheri Owen Detroit Neurotrauma Institute Wayne State University Detroit, Michigan (313)577-4648
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} A quick question. We do have Rhodin's Histology Text and Atlas } which serves us well. Is there anything similar available for SEM } sample identification? } Greetings, Johannes Rohdin's Histology is from 1974 and a SEM atlas I know is from 1979: Kessel RG and Kardon RH: Tissues and Organs, a text-atlas of scanning electron microscopy. W.H. Freeman and Company, San Francisco ISBN 0-7167-0091-3 Best regards. Sverker
********************************************************* Sverker Enestrom M.D., Ph.D. Department of Pathology University of Linkoping, Sweden Phone: +46 13 22 15 20 Fax: +46 13 13 22 57 *********************************************************
We currently employ an EPMA/SEM system for the analysis of semiconductors and packaging materials. Features on these materials are often less than one micrometer in size, which necessitates the use of low accelerating voltages to minimize the interaction volume. This leads to EDXS peak overlap situations, especially for N Ka/TiLa and SiKa/W Ma pairs. The use of WDX has been most beneficial for resolving these peak overlaps. However, our current LaB6 column lacks the resolution to adequately image submicron features and films.
We have considered replacing the LaB6 system with a field emission SEM equipped with a WDX spectrometer. Several vendors have demonstrated the ability of their FE guns to produce stable beam currents in excess of 15nA, which should be sufficient for WDX analyses. I would like to hear from any users of FE-SEM/WDX systems as to their experiences and possible recommendations. Thanks in advance.
Joseph M. Oparowski Digital Equipment Corporation 77 Reed Road, HLO2-3/J09 Hudson, MA 01749-2895 joseph.oparowski-at-hlo.mts.dec.com
Now the profit motive...I have more than an ounce of gold targets that I just can bear the thought of throwing in the trash can. Anybody ever tried to sell the spent targets?
} From: "Stephan Coetzee" {stephan-at-gecko.biol.wits.ac.za} } To: microscopy-at-Sparc5.Microscopy.Com } Date: Wed, 18 Dec 1996 12:41:23 GMT+2 } Subject: Texbook SEM Biological }
} Dear all=20 } } A quick question. We do have Rhodin's Histology Text and Atlas=20 } which serves us well. Is there anything similar available for SEM=20 } sample identification?
Is Rhodin's atlas is still in print in South Africa, I can't get it here in= the =20 US, it is out of print. For SEM are you aware of Richard G. Kessel and Ran= dt H.=20 Kardon 'TISSUES AND ORGANS: A TEXT-ATLAS OFSCANNING ELECTRON MICROSCOPY, Fr= eeman=20 ISBN 0-7167-0090-5 } } Thanks and Seasons Greetings to all } ## =20 } Stephan H Coeztee } Electron Microscope Unit } Private Bag 3 } Wits } 2050 =20 } South Africa } } Stephan-at-Gecko.biol.WITS.ac.za =20 } } Tell: +27 11 716 2419 } Fax : +27 11 339 3407
rschmitz-at-uwspmail.uwsp.edu or rschmitz-at-macsrv1.uwsp.edu=20 (note its macsrv"one" not "el") Robert (Bob) J. Schmitz Department of Biology,=20 University of Wisc. Stevens Point. Stevens Point, Wisconsin 54481 ph 715-346-2420
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} At 04:33 PM 12/17/96 -0400, you wrote: } } The strength of fields of this kind fall off as the third power of distance } } [i.e. B = f(1/r^3)], } } I have heard this a few times now, but can someone tell me why it is the 3rd } power? I thought the energy flux or intensity fell off as 1/r**2 from a } point source. The area over which the energy is spread increases with r**2. } For a line source the fall-off was 1/r, while for a plane source there was } no fall-off. } } Can you physics majors out there explain this to me. I know enough EE to be } dangerous. Is it that magnetic field strength is different? } ---------------------------------------------------- } Warren E. Straszheim
All-
Common EMF sources are almost exclusively monopole, dipole or quadrupole. The following analysis is technically 2-D since, among other reasons, a 3-D monopole is rather difficult to model. Fortunately, in all real-life EMF situations physical symmetry of the source in one or more dimensions allows 2-dimensional analysis.
Monopole magnetic field sources are the most pervasive and troublesome, and are typically due to stray currents where one part of the current loop is relatively near and the remaining segments of the loop are relatively far away. EMF distance-dependence for these cases is very close to 1/r.
Dipole magnetic field sources are those where the source and return current paths are in close proximity to each other with respect to the observer's distance. Such sources include well-isolated (i.e., not much leakage current) single- and three-phase transmission and distribution circuits. Scaling is independent of the physical size of the circuit (big transmission lines and home wiring are both in this category). EMF distance-dependence for this class of sources is the familiar 1/r^^2 relationship.
Quadrupole sources include transformers, fluorescent ballasts, motors and other devices such as VDT yoke electromagnets. Due to the presence of physically alternating multiple flux paths (which, incidentally, are only roughly predictable), these devices can emit locally strong fields, but the fields decrease rapidly with distance. The common EMF distance-dependence for these sources is 1/r^^3.
Higher-order components (hextupole, octopole, etc.) are often present, but drop off with correspondingly higher inverse powers and are typically inconsequential with regard to EMF's.
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I'm looking for a computer controlled XYZ stage control to attach to an inverted Nikon diaphot. Mac & IPlab compatablity are a plus. I already have info on the Ludl system. Anyone else have something different?
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I have a Carl Zeiss Photomicroscope I for sale. Asking price US$4,500.00 -- Dick Reinebeck, rreinebe-at-DIRECT.CA 2310 Magnussen Place, North Vancouver, B.C. V7J 3R6 (604) 984-6205
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{snip} } Monopole magnetic field sources are the most pervasive and } troublesome, and are typically due to stray currents where one part of the } current loop is relatively near and the remaining segments of the loop are } relatively far away. EMF distance-dependence for these cases is very close } to 1/r.
I was a little puzzled by this at first, so I went back and checked my old copy of Lorrain and Corson. For a "single long straight conductor", B does indeed fall off as 1/r and a dipole has a 1/r^2 field dependence. A dipole would be something like a solenoid (single or many turn) where you have distinct "North/South" ends.
Power lines usually have 2 (or more) conductors. So you can consider a power line to be a distorted (long and thin) solenoid loop and hence a dipole radiator. Intuitively, as you get farther away from it, the power line begins to look not like 2 wires, but like a single conductor with a low net current. (i.e. the field from each conductor tends to cancel other out, giving you a better than a 1/r dependence).
} } Dipole magnetic field sources are those where the source and return } current paths are in close proximity to each other with respect to the } observer's distance. Such sources include well-isolated (i.e., not much } leakage current) single- and three-phase transmission and distribution } circuits. Scaling is independent of the physical size of the circuit (big } transmission lines and home wiring are both in this category). EMF } distance-dependence for this class of sources is the familiar 1/r^^2 } relationship. } {snip} } } Best regards, } Curt Dunnam/C.U.
Cheers, Henk Colijn
Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility ------------------------------------------------------------------- Prosperity is the blessing of the Old Testament; Adversity is the blessing of the New. Francis Bacon, "Of Adversity."
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Hello All!
I have some pretty old JB4 resin components that haven't been refrigerated in ages. My question is...Can I catalyze this stuff and polymerize it as if it were new? This stuff is a lot cheaper to dispose of as a hard plastic than it is in it's basic components. So, is it safe to do this? Or will I make the 6:00 news by doing this?
Happy Holidays!
Paula Sicurello U.C. Berkeley Elevtron Microscope Lab
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We just found that our DAB is precipitating when we add to our secondary. Does anyone know how we can prevent this while still getting the DAB to react with our peroxidase?
Cheri Owen Detroit Neurotrauma Institute Wayne State University Detroit, Mi (313)577-4648
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REGARDING focused ion beam
We have a Gatan PIMS (precision ion milling system, scanned focused probe of Argon ions for etching selected regions) that we actually use from time to time. I understand that Gatan sold several units with an upgraded liquid metal ion source (LMS). Does anyone out there know who may have these units? Gatan has not been able to give me this information. We are considering this upgrade by purchasing a LMS from FEI. We believe that the lenses on the PIMS column will not be needed but we are not sure if the PIMS deflection system will work on these LM source of ions. Any comments are welcome. Either email of call.
Thanks in advance, happy holidays,
Mark A. Wall, L-350 Lawrence Livermore National Lab 7000 East Ave Livermore, CA USA 94550 ph 510 423 7162 email mark.wall-at-quickmail.llnl.gov
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You might try contacting Abe Dayani at Refining Systems Inc. He was willing to give us some credit on our old target.
Refining Systems, Inc. P.O. Box 72466 Las Vegas, NV 89170
702 368-0579 702 368-0933 FAX
No connection other than as a customer.
Cheers, Henk
} } Now the profit motive...I have more than an ounce of gold targets that } I just can bear the thought of throwing in the trash can. Anybody } ever tried to sell the spent targets? } } Jim Harper } jeharper-at-amoco.com
Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility ------------------------------------------------------------------- Prosperity is the blessing of the Old Testament; Adversity is the blessing of the New. Francis Bacon, "Of Adversity."
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Hi!
I have been testing four different softwares for 3D reconstruction: Voxelview Voxblast Spyglass Macstereology
Does anyone have any experience with these software packages?. I did find some publications about some of these programs, but it is not easy to get them. I am wondering if somebody have publications that can be faxed to me. I would pay if necessary.
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On Tue, 17 Dec 1996 wise-at-vaxa.cis.uwosh.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } I'm interested in pricing a chamber scope (scope and monitor and } whatever accessories are needed) for a Hitachi 2460N. Does anyone out } there in the yelm of microscopy have any recommendations? } Please respond directly to me and not the list. } } Thanks, } } Bob } } } Robert R. Wise, PhD } Director, UWO Electron Microscope Facility } Department of Biology } University of Wisconsin Oshkosh } Oshkosh, WI 54901 } (414) 424-3404 tel } (414) 424-1101 fax } wise-at-uwosh.edu } }
We have 2 of GW's infrared chanber cameras, one on a 2460-N. Both work quite well--they have saved many a trainees samples (and the final lens housing) from the bottom of the camber. About $3K I think. The image can be integrated into the 2460's display.
Tom
Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu) Central Microscopy Research Facility http://www.uiowa.edu/~cemrf 85 EMRB University of Iowa Iowa City, IA 52242 319-335-8142 FAX 319-335-8049
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Subject: Time: 4:15 PM OFFICE MEMO NRC on EMFs & Health Date: 12/18/96
As a final note on the possible relationship of magnetic fields to health problems, I just noted the following comment in the January 1997 issue of Scientific American (p. 3): "A committee from tne National Research Council has concluded that electromagnetic fields (EMFs) pose no real health threat, as was first alleged in 1979. The group surveyed more than 500 studies conducted over the past seventeen years investigating the link between EMFs and, among other diseases, cancer, reproductive abnormalities, and behavioral problems. They found that only exposures 1000 to 10,000 times stronger than what is common in residental settings could alter cellular function; in no study did EMF exposure affect cellular DNA. (See September 1966, page 80)." Wil Bigelow, Univ of Michigan !! (bigelow-at-umich.edu)
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Hi,
I have been asked to investigate the make up of a system that will be used to do infrared videomicroscopy, coupled with electrophysiology. I need some idea of space requirements for the physical setup. I also need to know if there are any special room requirements ie a darkroom. If you or anyone you know is involved with this type of activity I would enjoy hearing from you.
Thanks in advance,
Bob Kayton C.R.O.E.T. Oregon Health Sciences University Portland, OR kayton-at-ohsu.edu
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My personal theory (not yet published - please don't steal it) is that hip problems in microscopists are encountered when our hips and surrounding soft tissues are transferred from uncomfortable chairs in dark labs to uncomfortable stools in dark barrooms. The greater the frequency of this transfer, the greater the incidence of hip problems. Also, the greater the speed at which this seat-seat exchange occurs (i.e. proximity to the bar from the lab) is significant. I call this the "Lehigh Factor." There also seems to be a curious, as yet unresolved, trend toward increasing speed as a function of day of the week. Monday it is near zero. By Friday, the speed is best described as "break-neck."
I am entertaining potential grantors of funding for further research into these orphan phenomena.
Harry Crossman Osram Sylvania Inc., Beverly, MA and The Brewery Exchange, Lowell, MA
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I am interested in anyone with experience with high quality color printers. I already have a Codonics dye sub and am familar with what Tektronix has. I am thinking of the next step up from this type of printer. Is anyone familar with the Fuji Pictrography 3000? any other ideas? TIA.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (573)-882-4712 (voice) (573)-882-0123 (fax)
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Subject: Time: 5:44 PM OFFICE MEMO NRC Rpt on Health & EMFs Date: 12/18/96
As a final note on the possible relationship of magnetic fields to health prob#001##ms, I just noted the following comment in the January 1997 issue of Scientific American (p. 3): "A committee from the National Research Council has concluded that electromagnetic fields (EMFs) pose no real health threat, as was first alleged in 1979. The group surveyed more than 500 studies conducted over the past seventeen years investigating the link between EMFs and, among other diseases, cancer, reproductive abnormalities, and behavioral problems. They found that only exposures 1000 to 10,000 times stronger than what is common in residental settings could alter cellular function; in no study did EMF exposure affect cellular DNA. (See September 1966, page 80)." W. C. Bigelow, Univ of Michigan (bigelow-at-umich.edu)
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I have a question which I have been meaning to pose for some time now. I have noticed that when I collect a spectrum at 15KeV vs. one at 20 KeV, I get a different (very different) result from a standardless semi quant on each. The sample in question is a copper coupon used for corrosion testing with the elements copper, oxygen and sulfur. At 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and 13%S. Is this normal for a standardless analysis? I have asked the manufacturer of the EDS but have not received an answer. I would much appreciate it if someone could clarify this for me.
Thanks in advance.
Dave Strecker Rockwell Automation/Allen-Bradley Co.
P.S. Happy Holidays to all, and I hope to see you in Cleveland next August.
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We are planning to move our SEM into a room of its' own. Are there any special precautions we should take or things to look out for? I was wondering specifically about building vibrations. We have noticed a 17Hz vibration on the first floor of our building. Any suggestions will be welcome as I inherited an old SEM as a "project". TIA.
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A handful of times per generation, we mere mortals are witness to true eye-opening, jaw-dropping, why-didn't-I-think-of-that-?-(slap-on-the-forehead) genius. Harry, I am humbled in your cyber presence. No wonder my hips hurt so much (especially on Fridays). And here all these years I thought it was those errant electrons zipping around the lab.
Bob
Robert R. Wise, PhD Director, UWO Electron Microscope Facility Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (414) 424-3404 tel (414) 424-1101 fax wise-at-uwosh.edu
We have an Amray 1845 FE-SEM with an Oxford (Microspec) WD multi crystal spectrometer. It works very well for us as we often look at small samples and want to do high resolution imaging, surface imaging, BSE imaging and WDS / EDS in the same chamber (although at different working conditions). We run the Oxford Theta software as to combine ED and WD spectra (and collect simultaneously). Be aware of some practical issues, however. The Microspec WDS will be one crystal at a time and therefore slower than a conventional multi spectrometer microprobe. Also, I recommend you seriously consider thermally assisted field emission as opposed to cold field emission, as it will be more stable. Most importantly, remember that to do WDS AND maintain a small interaction volume, you will need the high currents, which means a large final aperture, low kV (we see very little drop in current from 20kV down to 5kV), and a long working distance (that is where most commercial designs I'm aware of operate). All this means poor spacial resolution, which may be a show stopper for you. Weigh carefully all the working parameters and available chamber designs with what you really want from the instrument you propose. All the best.
No endorsements should be inferred from anything I've said.
James P. Heuer, Ph.D. General Electric Co. (510) 862-4501 heuerj-at-vncpo1.ne.ge.com
Jim Harper wrote: ======================================================== } Now the profit motive...I have more than an ounce of gold targets that } I just can bear the thought of throwing in the trash can. Anybody } ever tried to sell the spent targets? ======================================================== The basic problem is this: That one oz. of gold, before it canb e "converted" back into "money" has to be analyzed by the refiner and the minimum analysis and refining cost is typically more than the value of gold in one ounce (or nearly so). So unless you have a dozen sputter coaters running side by side, all day long, a typical user would be unlikely to ever generate enough precious metals scrap to make it economic to send back to the refiner.
That is why we have offered a "trade in" program, send us back your spent cathodes and we will grant a 10% discount on a new one. When we have accumulated 20-30 troy ounces of spent cathodes (of a given metal), we then return them for refining and realize the economic value. This makes a lot of sense from a non-renewable resource standpoint as well, since once the world's supply of gold is gone, it is gone.
Note of caution: As with any commodity being set aside for recycling, do not mix cathodes of different compostions. Keep them separate! They have greater economic value separated than if they are mixed together and without traceability as to metal composition.
Chuck
===================================================== Charles A. Garber, Ph. D. e-mail: cgarber-at-2spi. com PRESIDENT SPI SUPPLIES/STRUCTURE PROBE, INC. WEST CHESTER, PA 19381-0656 USA
Look us up at xxxxxxxxxxxxxxxxx http://www.2spi.com xxxxxxxxxxxxxxxxx ====================================================
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} A quick question. We do have Rhodin's Histology Text and Atlas } which serves us well. Is there anything similar available for SEM } sample identification? } Sverker Enestrom M.D., Ph.D. **************************************** I note that the questioner is a pathologist. However, for any botanists I suggest that "An SEM Study of Green Plants" by John N.A. Lott, published by Mosby in St Louis 1976; ISBN 0-8016-3033-9 is excellent. - If still available. It's over 20 years now but I still love every picture. Jim Darley
Probing & Structure (Microscopy Supplies & Accessories) PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313 A great microscopy site: http://www.ultra.net.au/~pns/
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Dear Dave, you wrote: } I have a question which I have been meaning to pose for some time now. } I have noticed that when I collect a spectrum at 15KeV vs. one at 20 } KeV, I get a different (very different) result from a standardless } semi quant on each. The sample in question is a copper coupon used } for corrosion testing with the elements copper, oxygen and sulfur. At } 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and } 13%S. Is this normal for a standardless analysis? First, are you sure you set up the analysis parameters for the new conditions? The 15kV electron beam will not fully excite the Cu Ka x-ray line at 8.4 KeV, so if the Cu peak is lower, the other peaks will analyse as higher to make up the 100%. You always have to use sufficient over-voltage (at least double) for the highest energy peak you want to analyse. Of course, at this much over-voltage the accuracy of the O will be compromised, but you know you cannot win, only occasionally break even. Luck, Mary
Mary Mager Electron Microscopist Metals and Materials Eng., UBC 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel:604-822-5648, fax:604-822-3619 e-mail: mager-at-unixg.ubc.ca
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Dear All
We get our material from Argen Precious metals in Edenvale Johannesburg South Africa. (most currency is strong to the rand and may be of a advatage) They make up to order and may even cut and fit the target material to the target base (this is by hear say). The advantage is that they keep the waste and you may get a refund or you may not even have to pay for it!(if it is true!) They made to spec when ordered. To my knowledge 60% Pd 40% Au is preferable, suppose to produce a finer coating. . Tell: +27 11 609 8640 Fax: +27 11 452 3918 Standard disclaimer.
Best of luck. And seasons greetings
## [########] ## ## ## ## ## Stephan H Coeztee Electron Microscope Unit Private Bag 3 Wits 2050 South Africa
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Dear Jim, Gold is soft and easily worked. In Australia we have many local jewellers who can refashion gold into usable sputter targets. Try your local yellow pages. Gold plated onto a baser metal is a different matter.
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============================================================ POSITION OPEN - IMMEDIATELY
SCANNING ELECTRON MICROSCOPE (SEM) TECHNICIAN
An immediate SEM technician position is available at the NU's electron probe instrumentation center (EPIC). Duties include teaching and development of laboratories for undergraduate and graduate courses; training and assistance to users; and techniques and instrumentation development/modification. Preferred qualifications include a BS degree in materials related discipline or equivalent and hands-on experience with SEM, its related techniques and accessories (e.g.evaporators, sputtering and specimen preparation) - teaching and user training experience in materials area. Familiarity with modern electronics, computers, software and hardware, vacuum systems and vacuum machinery is desired. Please send resume with salary requirements to:
Human Resources Administration, Northwestern University Dept. E96-1029, 720 University Place, Evanston, IL 60208-1142.
Also send a copy of resume to:
Prof. Vinayak P. Dravid Materials Science & Engineering 2225 N. Campus Drive, MLSF Building Northwestern University, Evanston, IL 60208, USA Ph.: (847) 467-1363, Fax: (847) 491-7820 E-mail: v-dravid-at-nwu.edu
NORTHWESTERN UNIVERSITY is an equal opportunity, affirmative action educator and employer. ============================================================
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} We currently employ an EPMA/SEM system for the analysis of semiconductors } and packaging materials. Features on these materials are often less than } one micrometer in size, which necessitates the use of low accelerating } voltages to minimize the interaction volume. This leads to EDXS peak } overlap situations, especially for N Ka/TiLa and SiKa/W Ma pairs. } The use of WDX has been most beneficial for resolving these peak } overlaps. However, our current LaB6 column lacks the resolution to } adequately image submicron features and films. } } We have considered replacing the LaB6 system with a field emission SEM } equipped with a WDX spectrometer. Several vendors have demonstrated the } ability of their FE guns to produce stable beam currents in excess of } 15nA, which should be sufficient for WDX analyses. I would like to hear } from any users of FE-SEM/WDX systems as to their experiences and possible } recommendations. Thanks in advance. } } Joseph M. Oparowski } Digital Equipment Corporation } 77 Reed Road, HLO2-3/J09 } Hudson, MA 01749-2895 } joseph.oparowski-at-hlo.mts.dec.com
Unless you really need the energy resolution and sensitivity of WDX, isn't a standard 100kV/EDX TEM system is going to give you all the spacial resolution you need plus a lot of other opportunities and at a similar cost to the FE-SEM?
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} We currently employ an EPMA/SEM system for the analysis of semiconductors } and packaging materials. Features on these materials are often less than } one micrometer in size, which necessitates the use of low accelerating } voltages to minimize the interaction volume. This leads to EDXS peak } overlap situations, especially for N Ka/TiLa and SiKa/W Ma pairs. } The use of WDX has been most beneficial for resolving these peak } overlaps. However, our current LaB6 column lacks the resolution to } adequately image submicron features and films. } } We have considered replacing the LaB6 system with a field emission SEM } equipped with a WDX spectrometer. Several vendors have demonstrated the } ability of their FE guns to produce stable beam currents in excess of } 15nA, which should be sufficient for WDX analyses. I would like to hear } from any users of FE-SEM/WDX systems as to their experiences and possible } recommendations. Thanks in advance. } } Joseph M. Oparowski } Digital Equipment Corporation } 77 Reed Road, HLO2-3/J09 } Hudson, MA 01749-2895 } joseph.oparowski-at-hlo.mts.dec.com
Unless you really need the energy resolution and sensitivity of WDX, isn't a standard 100kV/EDX TEM system is going to give you all the spacial resolution you need plus a lot of other opportunities and at a similar cost to the FE-SEM?
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Dear Dave
I've never been involved with Standardless work, but around fifteen to ten years ago I did a lot of work with Link's Quantem and ZAF PB programs. With them you had to store profiles of elements and quantitative data from your known standards to derive sensitivity factors using any different kV's that you wanted to use on specimens, and dial these (corresponding to the chosen working kV) into an elements file to apply to unknowns. Maybe this bears on your problem, in that perhaps you should set-up and work on a chosen kV?
With best wishes - Keith Ryan
++++++++++++++++++++++++++++++++++++++++++++++++++ Keith Ryan Plymouth Marine Laboratory, Citadel Hill, Plymouth, Devon PL1 2PB, England
e-mail: k.ryan-at-pml.ac.uk PML web site: http://www.npm.ac.uk/pml ++++++++++++++++++++++++++++++++++++++++++++++++++
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May be you have oxydized surface layer.
On Wed, 18 Dec 1996, Dave Strecker wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } I have a question which I have been meaning to pose for some time now. } I have noticed that when I collect a spectrum at 15KeV vs. one at 20 } KeV, I get a different (very different) result from a standardless } semi quant on each. The sample in question is a copper coupon used } for corrosion testing with the elements copper, oxygen and sulfur. At } 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and } 13%S. Is this normal for a standardless analysis? I have asked the } manufacturer of the EDS but have not received an answer. I would much } appreciate it if someone could clarify this for me. } } Thanks in advance. } } Dave Strecker } Rockwell Automation/Allen-Bradley Co. } } P.S. } Happy Holidays to all, and I hope to see you in Cleveland next August. } }
Deborah: Most of this you probably thought off already , but here are some of the things I would do. I would ask the SEM manufacturer to do a survey of the room for vibrations and EM fields (they might charge you for this if they are not the ones who are moving the scope) The position of light fixtures are also important . Also, I would make sure that there is enough air conditioning capacity to cool your room and that the air ducts and vents are placed in the right strategic locations (i.e. as far away from the scope as possible). I would also check the labs which are adjacent to your new lab. What kind of equipment do they have ? will it interfere with your SEM ?. What about the lab upstairs ? We have had a few instances where accidental water leaks from the labs above us damaged some of our equipment. I hope this helps.
Jordi Marti ----------
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We are planning to move our SEM into a room of its' own. Are there any special precautions we should take or things to look out for? I was wondering specifically about building vibrations. We have noticed a 17Hz vibration on the first floor of our building. Any suggestions will be welcome as I inherited an old SEM as a "project". TIA.
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} } I have a question which I have been meaning to pose for some time now. } I have noticed that when I collect a spectrum at 15KeV vs. one at 20 } KeV, I get a different (very different) result from a standardless } semi quant on each. The sample in question is a copper coupon used } for corrosion testing with the elements copper, oxygen and sulfur. At } 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and } 13%S. Is this normal for a standardless analysis? I have asked the } manufacturer of the EDS but have not received an answer. I would much } appreciate it if someone could clarify this for me. } } Thanks in advance. } } Dave Strecker
This difference in analysis result may be real or artifact. It may be due to the larger (deeper) excitation volume at 20 kV, so that more Cu is excited relative to the surface O and S. Or it could be an artifact due to the standardless approach. I would suggest using a standards analysis and trying again. This should eliminate part of the problems (such as the overvoltage dependence suggested by Mary Mager)
Varying the voltage has long been used to analyze thin films relative to the substrate (as might be expected in certain corrosion systems). There is a freeware routine called "GMRFilm" on the Microscopy and Microanalysis software reference library at http://www.msa.microscopy.com/RefEdu.html. (the GMRFilm software can be found under ftp://ftp.msa.microscopy.com/pub/4-MMSLib/XEDS/) that allows one to analyze films using this method. There is also at least one commercial package which will fit the multivoltage data to determine the best composition of one or more films on a substrate. Both these packages will require the comparison of the unknown to standard bulk/polished materials. Monte Carlo modelling can also be used to understand the thin film/substrate compositions. There are several public domain monte carlo programs available also.
Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov Microanalysis Research Group Office: 301-975-3902 N.I.S.T. FAX: 301-216-1134 Bldg. 222/Rm A113 Gaithersburg MD 20899
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In message {0000FA68.1893-at-po.cle.ab.com} Dave Strecker writes:
} I have a question which I have been meaning to pose for some time now. } I have noticed that when I collect a spectrum at 15KeV vs. one at 20 } KeV, I get a different (very different) result from a standardless } semi quant on each. The sample in question is a copper coupon used } for corrosion testing with the elements copper, oxygen and sulfur. At } 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and } 13%S. Is this normal for a standardless analysis? I have asked the } manufacturer of the EDS but have not received an answer. I would much } appreciate it if someone could clarify this for me. } } Thanks in advance. } } Dave Strecker } Rockwell Automation/Allen-Bradley Co.
Dave,
Send me your FAX number and I will send you a two page reprint from the Microscopoy & Microanalysis '96 meeting held this past August in Minneapolis. I'll send you Dale Newbury's article entitled: "Standardless" Quantitative Electron Probe X-ray Microanalysis with Energy-Dispersive Spectrometry: What is the Distribution of Errors?
Dale (from NIST, Gaithersburg, MD) is one of the Microbeam Analysis Society's traveling speakers this year and for that his talk is entitled: Lies, Damned Lies, and "Standardless Analysis". I think from the title alone you get the gist of it. I've heard the talk and its quite revealing. The reprint I'll send you is a good introduction to the criticism of standardless analysis. Dale's conclusion is basically (in my own words): The Truth, the Whole Truth, and Standards-Based Analysis.
Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
"When the mode of the music changes, the walls of the city will shake." - PLATO "There's a whole lotta shakin' goin' on!" - CHUCK BERRY
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What wavelength region are you interested in?
There are some commercial sources for Near IR (0.7 to ~1 micron) video microscopy. I think I have also seen some commercial sources related to the mid-wave IR (3-5 microns) and/or long-wave IR (8-12 microns).
I know something about the camera systems available in the IR regions, if that would help.
At 01:57 PM 12/18/96 -0800, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} Subject: Time: 4:15 PM } OFFICE MEMO NRC on EMFs & Health Date: 12/18/96 } } As a final note on the possible relationship of magnetic fields to health } problems, I just noted the following comment in the January 1997 issue of } Scientific American (p. 3): } "A committee from tne National Research Council has concluded that } electromagnetic fields (EMFs) pose no real health threat, as was first } alleged in 1979. The group surveyed more than 500 studies conducted over the } past seventeen years investigating the link between EMFs and, among other } diseases, cancer, reproductive abnormalities, and behavioral problems. They } found that only exposures 1000 to 10,000 times stronger than what is common } in residental settings could alter cellular function; in no study did EMF } exposure affect cellular DNA. (See September 1966, page 80)." } Dear Wil, The bionet.emf-bio newsgroup had several comments about this report. Among them was the statement that the report actually said that there was no evidence for a health threat (Absence of evidence is not evidence of absence. :-) ). From what I've heard, there is some evidence that 60 Hz fields, especially, e.g., from electric blankets, has been correlated with statistical increases in childhood cancer. This evidence is tenuous, and not all experts are in agreement. The important features are that the field is produced near (~1 cm) the exposed person, and the person has a very long (~8 hr) exposure. Kershvinck (sp?) at Caltech has investigated the interaction of low- level EMF's with magnetite crystals associated with cell membranes, and he found that 10's of Hz is the order of magnitude for resonant frequencies. The energy calculations show that it is possible for such a crystal to affect a transport protein. The conclusion is that there is a physically possible mechanism for a low-level AC field to cause biological effects. For me the bottom line is that I would not use an electric blanket or other appliance that I'd be close to for long periods, but I'd be much more concerned over many other environmental hazards (air, H2O, food qual- ity, etc.). Yours, Bill Tivol
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} Unless you really need the energy resolution and sensitivity of WDX, isn't } a standard 100kV/EDX TEM system is going to give you all the spacial } resolution you need plus a lot of other opportunities and at a similar cost } to the FE-SEM? } Dear Larry, It all depends on what info is required. A TEM+EDX system uses a thin specimen which is penetrated by the beam, so the emission volume is determined by the incident beam size and the spread through the specimen. For our HVEM, the worst case, our incident beam is ~.4 micron, and the spread through a 1 micron section is about the same. This works well for ~1 micron resolution in x, y, and z, which is OK for biological sections. For 100 kV, the beam size is typically smaller, and the spread through a .1 micron section is also { { 1 micron. However, the emission volume is the entire thickness of the section. If surface measurements are needed, the SEM is a better choice. Yours, Bill Tivol
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} We are planning to move our SEM into a room of its' own. Are there any } special precautions we should take or things to look out for? I was } wondering specifically about building vibrations. We have noticed a } 17Hz vibration on the first floor of our building. Any suggestions will } be welcome as I inherited an old SEM as a "project". TIA. } Dear Deborah, Either of two possible mounting strategies can be chosen depending on the vibration environment. The column can be isolated from the building or it can be firmly anchored. If the building vibrations are large, the instrument has to be isolated, but if acoustic vibrations } } building vibs. then isolation may actually degrade performance. I have seen a TEM which could have been used as "The Clapper (TM)". Good luck. Yours, Bill Tivol
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It seems to me that a little perspective is called for in this, and any other debate on health risks.
If governments, consumer groups, etc applied themselves a little more to risks like tobacco, cars (direct and pollution), alcohol and guns then worrying about EMF might be might be relevant.
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Another posibility.... Is the the film analyzed thicker than the depth of you analysis volume? If the analysis volume extends into the Cu substrate more at 20 kV than at 15 kV (or less), then your results are understandable. _Woody_
Woody White http://www.geocities.com/capecanaveral/3722 ___________________________ Reply Separator _________________________________
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I have a question which I have been meaning to pose for some time now. I have noticed that when I collect a spectrum at 15KeV vs. one at 20 KeV, I get a different (very different) result from a standardless semi quant on each. The sample in question is a copper coupon used for corrosion testing with the elements copper, oxygen and sulfur. At 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and 13%S. Is this normal for a standardless analysis? I have asked the manufacturer of the EDS but have not received an answer. I would much appreciate it if someone could clarify this for me.
Thanks in advance.
Dave Strecker Rockwell Automation/Allen-Bradley Co.
P.S. Happy Holidays to all, and I hope to see you in Cleveland next August.
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Hello all,
I looking for a cell culture laboratory -preferable in Germany- but may be anywhere, that could supply me with "stock cell culture" material of Taricha granulosa (newt) lung cells. I have to generate some time lapse videos of its cell division in DIC/phase contrast-microscopy.
Thanks
Holger G. Adelmann {106421.3362-at-compuserve.com}
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Our college has purchased hundreds of microscopes and dissecting scopes over the years. The bulk of them are Wild/Leitz/Leica brand. Some have external transformers while others are built in. Last year we purchased 10 dissecting scopes with external transformers from Leica. The transformers were manufactured by Hammond Manufacturing (we have made similiar component purchases in the past). We have had all of these transformers fail, have returned them, had them replaced and the replacements have failed. Our maintenance dept looked at a few and noticed that the wire on the transformers is much thinner than the wire on the older models. We have never experienced this problem before. Leica is now refusing to replace these transformers and I have 10 non-functional transformers. If anyone has any suggestions I would appreciate hearing them. You can email me directly at nmcinerney-at-rdc.ab.ca.
I appreciate any advice anyone has to offer.
Nancy McInerney Red Deer College Red Deer Alberta 403-342-3142
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At 06:33 PM 12/18/96 -0500, you wrote: } I have a question which I have been meaning to pose for some time now. } I have noticed that when I collect a spectrum at 15KeV vs. one at 20 } KeV, I get a different (very different) result from a standardless } semi quant on each. The sample in question is a copper coupon used } for corrosion testing with the elements copper, oxygen and sulfur. At } 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and } 13%S. Is this normal for a standardless analysis? I have asked the } manufacturer of the EDS but have not received an answer. I would much } appreciate it if someone could clarify this for me. } } Thanks in advance. } } Dave Strecker } Rockwell Automation/Allen-Bradley Co.
Dave, Would you provide a few more specifics? It might help to know the manufacturer and software. If you tell us, I/we will try not to be too disparaging.
I presume you told the quant setup that you were changing kV. If you didn't, that would explain a lot.
We decided against a Kevex system back in the early 80's because its background treatment did not seem as good as Tracor Northern's at the time. But we bought a Kevex system in the late 80's after we were shown some more of its features. We were shown an absorption factor that enters into background modeling that can have a significant effect on the results. I suppose it factors into the correction the sensitivity for lines at different energies (since you do not have elemental standards). If a top-hat filter is used to remove the background, then the quant would/could not be tailored to fit your particular system's sensitivity, unless your system had somehow already been calibrated for that effect.
Having said all that, I will join with the others who will undoubtedly chime in and ask what do you expect for standardless analyses anyway? I often use them, but I have to remember they are only as good as the effort I invest in them.
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I was under the impression that Kessel's book was out of print??!!
Kathy Walters / / Research Assistant III / /\ Center for Microscopy Research / /\ \ University of Iowa /_/ \ \ 85 EMRB ____ ((O)) Iowa City, Iowa 52242 | | / / || / / email: kwalters-at-emiris.iaf.uiowa.edu ----------- fax: (319)335-8049 ------------- www: http://www.uiowa.edu/~cemrf
On Wed, 18 Dec 1996, Sverker Enestrom wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } } } A quick question. We do have Rhodin's Histology Text and Atlas } } which serves us well. Is there anything similar available for SEM } } sample identification? } } } Greetings, } Johannes Rohdin's Histology is from 1974 and a SEM atlas I know } is from 1979: Kessel RG and Kardon RH: Tissues and Organs, a text-atlas } of scanning electron microscopy. W.H. Freeman and Company, San Francisco } ISBN 0-7167-0091-3 } Best regards. } Sverker } } ********************************************************* } Sverker Enestrom M.D., Ph.D. } Department of Pathology } University of Linkoping, Sweden } Phone: +46 13 22 15 20 } Fax: +46 13 13 22 57 } ********************************************************* } }
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On Wed, 18 Dec 1996, DDHills wrote:
} Date: Wed, 18 Dec 1996 20:30:16 -0800 } From: DDHills {ddhills-at-mail.idt.net} } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: SEM Placement } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. } } We are planning to move our SEM into a room of its' own. Are there any } special precautions we should take or things to look out for? I was } wondering specifically about building vibrations. We have noticed a } 17Hz vibration on the first floor of our building. Any suggestions will } be welcome as I inherited an old SEM as a "project". TIA. } } Deborah Hills-Haney } Dr. Judy Murphy is an expert in this field (jmurphy-at-sjdccd.cc.ca.us). Maybe you should have her come in and look at your site. S.
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
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} } Send me your FAX number and I will send you a two page reprint from the } Microscopoy & Microanalysis '96 meeting held this past August in Minneapolis. } I'll send you Dale Newbury's article entitled: "Standardless" Quantitative } Electron Probe X-ray Microanalysis with Energy-Dispersive Spectrometry: What is the Distribution of Errors? } } Dale (from NIST, Gaithersburg, MD) is one of the Microbeam Analysis } Society's traveling speakers this year and for that his talk is entitled: } Lies, Damned Lies, and "Standardless Analysis". I think from the title alone } you get the gist of it. I've heard the talk and its quite revealing. The } reprint I'll send you is a good introduction to the criticism of standardless } analysis. Dale's conclusion is basically (in my own words): The Truth, the } Whole Truth, and Standards-Based Analysis. } } Gib Ahlstrand, MMS Newsletter Editor
Another good reference on the same subject:
Newbury, D. E., Swyt, C. R., and Myklebust, R. L., "'Standardless' Quantitative Electron Probe Microanalysis with Energy-Dispersive X-ray Spectrometry: Is It Worth the Risk?", Analytical Chemistry, 67 (1995) 1866-1871.
Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov Microanalysis Research Group Office: 301-975-3902 N.I.S.T. FAX: 301-216-1134 Bldg. 222/Rm A113 Gaithersburg MD 20899
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Dear colleagues,
We have to prepare Si TEM specimens by chemical etching technique. All our attempts failed due to the micro- or macro- pitting. We used a various combinations of HF, HNO3 and CH3COOH but without any success. Does anyone know the good content of the etching solution and regimes of chemical etching?
Thanks in advance. Happy Christmas and New Year.
Kirill Prikhodko Russian Research Center "Kurchatov Institute" Moscow E-mail: kirill-at-nw.oirtorm.net.kiae.su
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} Now the profit motive...I have more than an ounce of gold targets that } I just can bear the thought of throwing in the trash can. Anybody } ever tried to sell the spent targets? } } Jim Harper } jeharper-at-amoco.com
I suspect the answer is the same as for all those people whoe save their Pt apertures or want to recycle the silver in their dark rooms - unless you are in a situation where you can operate on an appropriately large scale, the time and effort simply doesn't justify the return.
Message-Id: {2.2.16.19961220154819.1c6f9d62-at-bcm.tmc.edu} X-Sender: dturner-at-bcm.tmc.edu X-Mailer: Windows Eudora Pro Version 2.2 (16) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
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I need information regarding processing for thin-sectioning of liposomes. These samples are used for Cyclosporin A treatment (inhalation) of lung tumors. I will also be using negative staining. Suggestions please!
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Please unscribe Cristiano Rumio
Dr. Cristiano Rumio Istitute of Anatomy University of Milan Via Mangiagalli 31, 20133 Milan Italy E-mail clsmteam-at-imiucca.csi.unimi.it Voice: -39.2.2663683 Fax:-39.2.2364082
We've been backside etching Si with HF-Nitric-Acetic (H:N:A) since the 1950s. Formulas have changed over the years. Currently we use H:N:A = 6:10:5. Various ratios of the components produce etches that are faster or slower. Myrtle Ellington wrote a fine paper on this in MRS Symposium Proceedings 115, p. 265, where she had different etch rates with different formulations used in the beginning, middle, and at the end of the etching procedure. Her ratios H:N:A = 4:1:2 initally, 10:1:0 and then 15:1:0 for the final etch. Obviously there is a wide range of formulas that etch Si.
With our 6:10:5 formula we start etching with fresh etchant and see rapid thinning with a lot of bubble evolution. For the final, smooth, bubble-free etching step we use old solution in the same ratio that is a couple of weeks old. We mask the Si in wax* on a glass microscope slide and apply one drop of etchant at a time. In the early, bubbly, stage we remove the etch drop with a Q-tip (cotton bud) after a few seconds.** In later stages the old solution can be left on the Si for up to 30 seconds.***
We tripod polish the plan view Si from the back until the Si is 200-300um thick before etching. With Si this thin, etching is finished in a couple of minutes.****
* Use real beeswax, from bees, dissolve in xylene. If a lot of ion milling is anticipated use a cyanoacrylate (super glue) instead of wax. ** Alternatively, especially in later stages, remove the old etchant by dipping the entire slide in distilled water and blow dry. Allows for safe microscope examination. *** At the very, very end, while you are monitoring the extent of the thin area, leave the etchant on for very short times between examinations. Let it go for 30 seconds only when it is safe to do so. ****Thin starting Si allows for low angle ion milling the hole side. Would get the same result with large wheel dimpling a'la Helen Humiston.
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There seem to different ways to label oligonucleotides without using radioactive probes. There are few companies that carry products for it. Could someone please tell me which is their preferred method and which company they deal with. Thanking you in advance,
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Dear Dr. Prikhodko:
A technique was developed many years ago by one of our customers for chemcially thinning silicon for TEM. The process does involve the use of our Model 550D Single Vertical Jet Electropolisher which has been set up in chemical etching mode.
The process involves using a polishing solution of 360ml nitric acid and 90ml HF. The jet nozzle is placed 3 to 4mm above the sample and the flow rate set as slow as possible without breaking the solution into drops above the sample. In electrolytic mode, we would typically use an infrared LED in our detector circuit, but since silicon is transparent to infrared, we replace the infrared with a green LED to allow us to automatically terminate the process.
Some of this may not make sense as most people are familiar with the twin-jet configuration. The Model 550D does not immerse the sample in the solution - it sits on a pedestal (sounds appropriate for a very important sample!) and the solution is flowed onto the sample from the top side only. While it may sound inefficient to polish from just one side, there are actually a significant number of advantages which has allowed us to prepare samples that were previously impossible with a twin jet system. I'd be pleased to discuss these points off-line with anyone who has an interest.
If you would like to receive a copy of the procedure for thinning silicon, please contact me and I can FAX and/or mail a copy of the procedure to you. I also have a bibliography of technical reports dealing with TEM sample preparation. I would be pleased to send this to anyone who has an interest. You can then request reprints of any of the papers and we will send them out to you at no charge.
I hope this helps!
Best regards-
David Henriks TEL: 800-728-2233 (toll-free in USA) South Bay Technology, Inc. 714-492-2600 1120 Via Callejon FAX: 714-492-1499 San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
************** PLEASE VISIT OUR WEB SITE! **************
http://www.southbaytech.com
Manufacturers of Precision Sample Preparation Equipment and Supplies for Metallography, Crystallography and Electron Microscopy.
YOUR MESSAGE: Dear colleagues,
We have to prepare Si TEM specimens by chemical etching technique. All our attempts failed due to the micro- or macro- pitting. We used a various combinations of HF, HNO3 and CH3COOH but without any success. Does anyone know the good content of the etching solution and regimes of chemical etching?
Thanks in advance. Happy Christmas and New Year.
Kirill Prikhodko Russian Research Center "Kurchatov Institute" Moscow
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Reply to: RE} TEM: Si chemical etching
Kirill: It is not the ratio of acids which caused problem. I used to do chemical etching in Taiwan. It went fine. However, the same recipe resulted in pitting on Si(100) but not on Si(111) when I tried it in New York. It seemed that preferential etching happened. Even when I did it in clean room or change recipes, it did not solve the problem. I suspect the possible reasons are the contents of the acids (concentration, contaminants, etc.), temperature, sample holders (Teflon versus glasses??), wax, etc. You may only need to change the vendors of the chemicals. I would also like to know the real cause though I do not do chemical ethcing any more.
Tan-Chen Lee Materials Characterization Lab Motorola, Inc. --------------------------------------
Dear colleagues,
We have to prepare Si TEM specimens by chemical etching technique. All our attempts failed due to the micro- or macro- pitting. We used a various combinations of HF, HNO3 and CH3COOH but without any success. Does anyone know the good content of the etching solution and regimes of chemical etching?
Thanks in advance. Happy Christmas and New Year.
Kirill Prikhodko Russian Research Center "Kurchatov Institute" Moscow E-mail: kirill-at-nw.oirtorm.net.kiae.su
------------------ RFC822 Header Follows ------------------ Received: by mesaqm.sps.mot.com with SMTP;20 Dec 1996 07:38:13 U
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I have an Hitachi H-7000 with SEM/STEM mode. I am in need of an x-ray detector and associated hardware to do elemental analysis (EDAX or EDS or whatever the proper generic term is). As a small 4-year college, we would much rather go the route of purchasing a used system.
Please send any replies directly to me. Thank you.
______________________________________________________________________ Donald L. Lovett e-mail: lovett-at-tcnj.edu Assoc. Professor, Dept. of Biology voice: (609) 771-2876 The College of New Jersey * fax: (609) 771-2674 Trenton, NJ 08650-4700
(* formerly Trenton State College; please note our new name)
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I would very much appreciate some information on a Carl Zeiss light microscope. This is an older model binocular unit with a five objective nose piece. The two eye pieces are marked 8x KPL. What is KPL? The five objectives lenses are 10x/0.22, 16x/0.40, 25x/0.60, 40x/0.65 and 100x/1.30-NEOFLUAR (what is NEOFLUAR?). I don't know if the 100x is oil or not. There is a number on the top that looks like 4254092. Near the eye piece is 4656721. On the base is 4291647. Near the filter holder is 4653272. There are two filter holders, one with a clear glass and one empty. The condenser has a rack and pinion for movement and a swing out lens marked ".9". The built-in light source has an external AC power transformer/controller with "TYP 392524" and "Regel Transformator" marked on it. So, with all that, can you tell me the age and worth of this microscope. Also, the availability of replacement parts. Will this unit take standard eye pieces and objective lenses etc.? Any other information you can offer will be most helpful. Thank you in advance for your time and help.
R.H. Isabelle 4 Pioneer Way Springfield, MA 01119-1720 E-mail: bobcat54-at-aol.com
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Hello all,
I am looking for a device called "Rotocompressor". It is a kind of chamber to put onto the microscopic stage to observe protists or other cells, while being able to rotate them into position and/or applying some pressure to them in order to flatten them to observe specific detail. I think it was first used by American protozoologists.
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Hi Lucille, I guess you know the SIMS is ready to go. The job was uneventful except for the fact that I slipped on the ice and fell down the motel stairs. I guess a little extra padding is good for something. While on the subject of physical things, I hope your cold is better. You really sounded awful.
I wanted to get there between the SIMS and Christmas but I have had three service calls since my return. I really have to hire somebody to help me. Clearly, the business is growing and I can't stretch much farther.
Please recall that you were going to send a drawing of the rooms which I am to survey. A reduced (small) general diagram would do just fine. I guess you received my revised quote. I hope it is acceptable.
Well...have a great Christmas and a Happy, Healthy New Year.
Alex Greene Scientific Instrumentation Services, Inc. Austin, Texas
At 07:39 AM 12/11/96 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --
With regard to Kirill Prikhodko's request, a recommended procedure for preparing Si is by chemical jet etching. The solution used is typically HF based. There are many which are quite sufficient. The following are a few which have been proven satisfactory:
1.) 90% Nitric acid 10% HF 2.) 5 parts Nitric acid, 3 parts Acetic acid, 3 parts HF. Polish at room temperature. 3.) 10.5 grams Potassium Permanganate, 300ml HF, 30ml De-ionized Water. Polish at -20 to -30 degrees C with a high jetting speed.
Depending on the orientation of the Si, the percentage of the acids may need to be altered.
These solutions and conditions have provided excellent results when utilizing the twin-jet electropolishing technique which simultaneously thins both specimen surfaces. If it is desired to back-thin the Si, one side should be protected with Beeswax.
For the electrolytic polishing of metals it is recommended to have both the specimen and the jets submerged in the electrolyte by approximately 3-4mm. When chemical etching of Si, it is recommended that the specimen be above the chemical solution level. The jet position in relation to the specimen can be varied to provide the necessary configuration of the dimple produced by the chemical etching process.
Kind regards for a Happy Holiday Season,
Paul Fischione
E.A. Fischione Instrument, Inc. is the manufacturer of the Automatic Twin- Jet Electropolisher.
The best technique to analyse liposomes is cryo-TEM, by the direct observation of a thin frozen (actually vitrified) hydrated suspension. Alternatively, you may use the freeze fracture replicas approach. Good luck.
Regards, Michel
**************************************************** Michel Deschuyteneer, Ph.D. deschuyt-at-sbbio.be Scientist Electron Microscopy Laboratory
SmithKline Beecham Biologicals Rue de l'Institut, 89 B1330 Rixensart, BELGIUM Tel: +32-2-656 9290 Fax: +32-2-656 8164 **************************************************** Standard disclaimer: the opinions expressed in this communication are my own and do not necessarily reflect those of SmithKline Beecham. ****************************************************
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In recent weeks, I have had so many inquires, I thought I should send a general e-mail.
Below is i. reminder notice regarding 2nd Northern Workshop on TEM Sample Preparation of Thin Films - ceramics, metals, semiconductors-
ii. 1st flyer for same.
___________________________ reminder
2nd Northern Workshop on TEM Sample Preparation of Thin Films - ceramics, metals, semiconductors-
Link|ping University, Link|ping, Sweden
February 25-28, 1997
Deadline for registration and abstracts: January 10, 1997; limited entries may be accepted up to February 20, 1997
Our original mailing list included Sweden, Denmark, Norway, Finland, Canada, USA, Belgium, France, Germany, The Netherlands, Hungary, Switzerland, Ireland, England, Japan and Australia. Additional inquiries have led to (further) distribution in the Ukraine, UK, USA, France, India, Belgium, Spain, Russia, Denmark and Sweden. Additionally, we have advertised at the following conferences: EUREM, AVS, SCANDEM, as well as by several electronics means. -} Number of registrants will be limited.
Second and Final announcement with the Preliminary program will follow after the above deadline in January 1997
Advance payment can be made via Swedish Post Giro to account 183415-9 Link|ping University. Mark the pay-slip with the project number 12276001 !!
On-site payment can be made in Swedish or USA cash only. ___________________________ Call for Papers & Registration, revised 1st Announcement 2nd Northern Workshop on TEM Sample Preparation of Thin Films - ceramics, metals, semiconductors-
Link|ping University, Link|ping, Sweden
February 25-28, 1997.
Basic and Advanced Sample Preparation Techniques Oral and poster presentations
Invited Speakers Gy|rgy Radnoczi, Res. Inst. for Tech. Phys. of Hungarian Academy of Sciences, Low Angle Ion Beam Technique; Practice and Theory for Cross-sectional Samples
Tom Malis, Materials Technology Laboratory, Natural Resources Canada, Microtomy for the Material Sciences
Ron Anderson, IBM East Fishkill, NY USA, Tripod Polishing and FIB Techniques for Precision TEM specimen preparation
Michael Phaneuf, Chipworks Inc., Ottawa Canada & Link|ping University, TEM Replication Techniques Applied to Semiconductor Dopant Profiling; Dental Composites for Low-Temperature XTEM of Galvannealed Steels
Equipment Exhibit (including all types of microscopy tools and instruments)
Demonstrations and Hands-on Laboratory Sessions (will occupy 50% of scheduled workshop time)
Exhibitors / Sponsors NorFA Network on "Materials Studies with Electron Microscopy Studies" Nanometric Systems & South Bay Technology, Inc., Scandinavian Society for Electron Microscopy, Atema Instruments (representing Gatan, Inc.), Pfeiffer (representing BAL-TEC and Balzers), E.A. Fischione Instruments, Inc. Technoorg Linda Ltd. Co Link Nordiska AB, Diatome Ltd., Micrion, Philips, RMC, Leica, FEI.
'Beamer' - an Informal Gathering of 'Electron-Beam Workers'
For further information on this workshop, please contact the organizers: Lynnette Madsen Lars Hultman Dept. of Physics (IFM) e-mail: lyn-at-ifm.liu.se lhn-at-ifm.liu.se Link|ping University voice: +46-13-284479 +46-13-281284 S-581 83 Link|ping, SWEDEN (English) (Swedish or English) fax: +46-13-137568
http://www.ifm.liu.se/Thinfilm/html/news.html#wshop Demonstrations and Hands-on Laboratory Sessions (will occupy 50% of scheduled workshop time) Use of low angle ion beam milling equipment Mechanical preparation methods Microtomy for materials science Chemical Methods Polishing Cleaving Replication Includes access to TEM for examination of prepared specimens
Our Equipment o precision low-angle ion miller (BAL-TEC RES 010) ion miller with cooling (Gatan Duo Mill Model 600), polishing wheels (e.g. Buehler ECOMET 4) tripod polisher (South Bay Technology Model 590) cleaving equipment (incl. Fischione hand disc grinder 660). dimpler (Fischione 2000), wire saw (South Bay Technology), standard diamond saw, ultrasonic disc cutter (Gatan 601) plus other standard and older pieces of equipment. o high-resolution 200 kV microscope with point-to-point resolution of 1.9 ] (Philips CM20 Ultra-Twin) with EDS analytical facility, 120 kV Philips EM400 T microscope 45o tilting facilities o Additional equipment, e.g. ultramicrotomes, will be available for the duration of the workshop to facilitate learning a wider repertoire of techniques.
Laboratory Visits on the 28th (optional) Several institutions in Sweden are involved with electron beam techniques. We encourage all participants to make the most of their trip to Sweden and invite them to visit Sweden's facilities. Industriellt Mikroelektronik Centrum AB , (IMC), Link|ping Ulf Wahlstr|m +46-13-281299 or fax+46-13-282200 Chalmers University, Physics Department Dr. Eva Olsson +46-31-7723316 or fax +46-31-7723224 Lund University, Chemical Centre, Inorganic Chemistry, Dr. L. Reine Wallenberg +46-46-108233 or fax +46-46-104525 Uppsala University, Department of Materials Science, Dr. Stefan Johansson, +46-18-183086 or fax +46-18-555095
Second and final announcement and Preliminary program January 1997
Language of the workshop is English. Number of registrants will be limited.
Accommodation Prices quoted are approximate for a single/double SEK per night, * indicates special prices for the workshop if you identify yourself at the time of booking as a participant. Please make your own reservations. Valla Folkh|gskola (+46-13-146860 voice) on campus at 340/500; Park Hotel* (+46-13 -129005 voice or 46-13-100418 fax) near the train station at 700/800, Hotel du Nord* (+46-13-129895 voice or 145291 fax) near the train station at 555/650, Good Evening Hotel (+46-13-129000 voice or 46-13-138850 fax) downtown at 555/666. If you need more information please do not hesitate to contact us.
Telephoning: Dial 08-rest of number in Sweden, 46-8-rest of number outside of Sweden Hotel prices are not guaranteed. Please make your own reservations. Buses run from Arlanda to the train station during the daytime and early evening. A train can be taken from Stockholm to Link|ping (~2.5 h). See reverse side for abstract format
I wish to register for the Main Programme SEK 2000 o Beamer only SEK 500 o cost of beamer is included for all main programme registrants Equipment Exhibitor Fees: } 1000 employees worldwide US$ 1000 or SEK 6000 o {1000 employees worldwide US$ 500 or SEK 3000 o includes entrance to conference and beamer for up to 3 persons, and a one page advertisement in the workshop report I wish go on the tour of Gamla Link|ping (outdoor museum of historical interest, cost included in workshop fee) o
I would like to give a oral seminar / presentation o present a poster o assist with hands-on laboratory sessions o participate in the equipment exhibit o ASSIST IN ANY WAY NEEDED o Reduced fees apply to persons helping with the conference.
Deadline: January 10, 1997; limited entries may be accepted up to February 20, 1997 Surname: ___________________________________________ First Name: ___________________________________________ Title: ___________________________________________ Institution: ___________________________________________ Telephone : ___________________________________________ Fax : ___________________________________________ e-mail: ___________________________________________ Address: ___________________________________________ ___________________________________________ ___________________________________________ if applicable, title of presentation: ________________________________________________________ ________________________________________________________ Indicate whether you prefer a poster or oral presentation: poster- 1 board o 2 boards o oral- desired duration ____ minutes Is this original or recent research? _____ Do you plan on submitting a full length manuscript? ______
Indicate the laboratory skills you would like to learn (number in order of importance) Mechanical preparation methods incl. ion beam milling ______ Polishing ______ Cleaving ______ Use of low angle ion beam milling equipment ______ Microtomy for materials science ______ Chemical Methods (e.g. MgO thinning) / Carbon Replica ______
Are you interested in sessions on: How to choose your techniques? ________ How to set-up your laboratory? ________
Call for Papers o original papers can often be accommodated in either an oral or poster format according to the authors wishes (time permitting): o posters of an instructional nature previously displayed are also welcome; original presentation site to be included on the display and in workshop report. Poster boards 118 cm by 118 cm in size provided. 1 or 2 boards may be used, however, note that a fold in the boards occurs at the dividing point in each pair. o Abstracts (regular or extended) should be submitted by the above deadline. A format is suggested on page 4. Please provide the fax number and e-mail address (if possible) of the person presenting the paper. 3 cm margins are recommended.
Travel Within Sweden: by train or car
From farther away: o Link|ping has a small airport (code JZ). o It may be more convenient to fly into Norrk|ping (with connections to Copenhagen and Stockholm) and take a taxi to Link|ping (for a cost of approximately ~300 SEK and best arranged in advance: voice +46-13-160939, fax -163151). o If you fly into Arlanda-Stockholm airport then you may need to stay overnight:
Arlanda Good Morning Hotel, Box 51, 190 45 Stockholm-Arlanda, telephone +46-8-65501000, Single room ~695:- / Double room ~795:- SEK Arlandia Hotel SAS, Arlanda Flygplats, Box 103, 190 45 Stockholm-Arlanda, phone +46-8-59361800, fax +46-8-59361970, Single room ~1250:- /Double ~1450:- SEK
Stockholm (near train station) Prize Hotel, Kungsbron 1, Stockholm, telephone +46-8-149450, fax +46-8-149848 Single room ~800:- / Double room ~985:- SEK, Hotel Adlon, Vasagatan 42, 111 20 Stockholm, telephone +46-8-245400, fax +46-8-208610 Single room ~715 to 895:- / Double room ~1050:- SEK Stockholm Central Hotel AB, Vasagatan 38, S-111 20 Stockholm Sweden, phone +46-8-22 08 40, telefax +46-8-24 75 73, single room ~650 to 1075:- / double room ~650 to 1275:- SEK
Format for abstracts (title and names, affliation centered; abstract left-justified)
TEM Sample Preparation of Material Y by Technique Z
Jane E. Smith Company ABC, Inc., Address
John F. Doe ABC University, Address Fax: 12-344556, e-mail: doe-at-abc.unive.nw
Copies of the abstracts will be made available at the workshop in the form of a Link|ping University report which can be referred to. Full articles associated with the conference will be gathered for a special issue of the fully refereed journal, Micron in the Jan.-Feb. 1998 issue. Manuscripts should be submitted at the workshop. The regular review procedure will be followed.
_____________________ Merry Christmas and a Happy New Year
Institutionen f|r Fysik och M{tteknik (IFM), Fysikhuset Link|pings Universitet 581 83 Link|ping, SVERIGE 013-284479
Department of Physics (IFM), F-house Linkoping University '!/ S-581 83 Linkoping, SWEDEN +46 13 284479 -at- -at- +----------------------------------------oOO-(_)-OOo---------+
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On Tue, 17 Dec 1996 wise-at-vaxa.cis.uwosh.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } -----------------------------------------------------------------------. I had a demostration of PCI image system from Hitachi at my E.M. Unit a year ago. I was quiet impressed about how easy to capture the image from my Hitachi S-2500 SEM and replay back to camera for photo quality picture. Now acturally I just purchased the sytem a week ago. I played around a bit and found it is even better than what I thought. In the version 4 of the system , it is more easy to get 3-D image and color the images capatured from SEM. I plan to introduce it to my clients to use it as daily research and get instant images on printer or store in a zip drive 100 MB disk in the new year.
Merry Christmas and a Happy New Year !!!
} To all, } } Portions of this subject have been discussed over the past few } months but I haven't really been paying close attention to it. I apologize } in advance if we rehash ground already covered. } We have a (an?) Hitachi 2460N SEM and I'm looking into outfitting } it for digital image capture (with annotation, file storage, printer, etc). } I know that Hitachi sells a system that is supposed to plug right in and } comes with a lot of bells and whistles. Has anyone had any experience with } the Hitachi system? Alternatively, does anyone know of an after market } product that performs well? Given that I am a computer dummy, I would be } more inclined to purchase a total system rather than attempt to put one } together on my own from individual components. } Please respond directly to me and not to the list. } } Thanks in advance, } } Bob } } } Robert R. Wise, PhD } Director, UWO Electron Microscope Facility } Department of Biology } University of Wisconsin Oshkosh } Oshkosh, WI 54901 } (414) 424-3404 tel } (414) 424-1101 fax } wise-at-uwosh.edu } } }
*********************************************** * Ming H. Chen, PhD * * Medicine/Dentistry Electron Microscopy Unit * * University Of Alberta. * * Edmonton, Alberta, Canada * ***********************************************
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Hello all,
I have to generate video files from lung cell division of Taricha granulosa (newt) in phase, DIC and fluorescence. Is anybody out there - preferably in Germany, but may be anywhere on this small planet - that could provide me with stock material of this cell line to get it cultured ?
Thanks
Holger G. Adelmann {106421.3362-at-compuserve.com}
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Hello all,
I'm looking for a device that is called Rotocompressor. It fits onto the microscope stage and was originally constructed by American protozoologists. It could be used to observe protists and cells in general while rotating them to any position wanted and applying defined pressure to them in order to get them flattened. Is anybody aware of such a device and a manufacturer ?
Thanks
Holger G. Adelmann {106421.3362-at-compuserve.com}
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FORWARDED MAIL -------
Dear Microscopy List -
We are interested in doing peak deconvolution of an x-ray spectrum. Our instrument has a routine that performs automatic background subtraction and then a least squares minimization using standard files to deconvolute the unknown peaks in the spectrum. However, the method is limited to a maximum of 15 elements. To automate the method for unknown analysis we would like to have at least 30 elements, and perhaps as many as 40. Of course, all available x-ray lines from 1 keV to 30 keV will be of interest.
Does anyone know of available computer programs to perform this data reduction? We would like something in the public domain, but would be willing to buy a commercial package if necessary. We would also welcome any recommendations from the literature.
Thank you in advance for any input you can give us.
D. Clark Turner MOXTEK, Inc. 452 West 1260 North Orem, Utah 84057
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On Tue, 26 Nov 1996, Goran Drazic wrote:
} Date: Tue, 26 Nov 1996 15:30:21 +0100 } From: Goran Drazic {goran.drazic-at-ijs.si} } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Asbestos: tremolite/chrysotile ratio determination } } } Dear Microscopists, } } I am looking for a (practical) method or procedure for the determination } of tremolite/ chrysotile ratio (volume, mass, number of fibers) in bulk } samples (asbestos-cement products). I will be very grateful if you are } willing to share any experience regarding this matter using optical M, } SEM, TEM, XRD or classical analytical chemistry. } } Best regards, } } } Dr. Goran Drazic } Ceramics department } J. Stefan Institute, University of Ljubljana } Slovenia } Try contacting Dr. Victor Roggli, Pathology, Box 3712 Duke Med Ctr, Durham, NC 27710; 919 286 0411 X6615. (Expert on asbestos).Not sure of email address. Try roggli.victor-at-forum.va.gov or maybe roggli-at-forum.va.gov
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
A belated thank you to everyone who responded regarding AC noise question. As many of you suggested the problem was caused by vibration. We worked with our Philips engineer to deduce the source of the problem.
The column is on a slab and one of the steel bars holding the floor was making contact with the slab and this transmitted the vibration. We had to take the floor boards apart and it was easy to see where it was touching. Now the scope is doing much better. If anyone would like a summary of responses, please e-mail me.
Thank you and have a good new year.
Mohan Kalyanaraman Mobil Technology Company PO Box 480 Paulsboro, NJ 08066 609-224-3989 mxkalyan-at-pau.mobil.com
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I have a Zeiss Epifluorescent Illumination condenser tube with the filter/beam spliter for sale. It is for the 160 mm tube length scopes, pictures are available for downloading upon request from serious buyers. Excellent condition, does not include reflector housing or lamp. Currently priced in the US at over $3200.00. Will sell for $2000.00 US. Pursuing a masters degree and have references.
Mr. John K. Weaver 707 839-5651 ,California, USA jkw1-at-axe.humboldt.edu John K. Weaver, jkw-at-axe.humboldt.edu Department of Biological Sciences Humboldt State University, Arcata, CA
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On Wed, 18 Dec 1996, Cheri Owen wrote: } Yuck! We have DAB precip on our immunocytochemistry sections. They are } Araldite embed sections on slides that we have etched and circled with PAP } pen. Has anyone else had this problem and do you have any solutions? Is } it possible to get the precip off once it has formed? We haven't had any } luck. Any suggestions would be greatly appreciated. } } Cheri Owen } Detroit Neurotrauma Institute } Wayne State University } Detroit, Michigan } (313)577-4648 } Dear Cheri: Do you get the precipitate if you do not use the pen? The DAB may also contain impurities as explained in: Pelliniemi et al., 1980 J. Histochem. Cytochem. 28:191-192. I hope this will be of help.
---------------------------------------------------------------------------- I Dr. Lauri J. Pelliniemi Telephone +358-2-333 7312 I I Associate Professor or +358-2-333 7209 I I Laboratory of Electron Microscopy I I University of Turku Telefax +358-2-333 7380 I I Kiinamyllynkatu 10 Internet Lauri.Pelliniemi-at-utu.fi I I FIN-20520 Turku http://www.utu.fi/research/crede/pelliniemi.html I I FINLAND, Europe http://www.utu.fi/med/em/index.html I ----------------------------------------------------------------------------
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Hello Fellow Microscopists, I have made serial sections of guinea pig inner ear and need some help at this to register the sections in order to make a 3D reconstruction. The sections are approx 0.7 micron, stained and photographed with a digital camera. There are fifty images stored in tif format each being 4.6 megs so I cannot include examples of these images to the entire newsgroup. The sections were cut from tissue (normal control) used in another experiment and are no way involved with my research commitments to the lab. It is an "after hours" personal project I have been meaning to finish but cannot find the time just now to handle each of the sections. If anyone would be interested in helping me to stack these images I think we could make a quite nice reconstruction. and would be glad to discuss it with you. I hope you all have a really Happy New Year! Sincerely,
Gary Zajic Kresge Hearing Research Institute Biochemistry Laboratory 1301 E Ann Ann Arbor, MI 48109 zajic-at-umich.edu
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