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From: joseph-at-marketcom2.com
Date: Mon, 1 Sep 1997 10:17:46 -0400 (EDT)
Subject: Automatic Search Engine Registration Software

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From: Martin Kohler :      mk-at-enk.ks.se
Date: Mon, 1 Sep 97 16:50:59 +0200
Subject: Video presentation equipment?

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Dear microscopist,

I am looking for the best options on the market for hardware and
software to create video presentations. I want to be able to create video
from series of TIFF images (or whatever format one uses) and edit these
videos, add text and effects of different kinds...

I would appreciate relevant comments from you about:
- Hardware for this that goes with a PC
- Software for this that runs under Windows NT 4.0

I am simply interested to hear what you may use for this and comments
about it.

Thanks,
Martin

-----------------------------------------------
Martin K=F6hler
Department of Molecular Medicine
Rolf Luft Center for Diabetes Research L6B:1
Karolinska Hospital
S-171 76 STOCKHOLM
SWEDEN
phone 46-8-51775732
46-8-51775727
fax 46-8-51773658
E-mail mk-at-enk.ks.se
---------------------------------------------





From: bozzola-at-siu.edu (John J. Bozzola)
Date: Mon, 1 Sep 1997 19:50:26 -0600
Subject: detection limits x-ray

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Under ideal (and reasonably attainable conditions), what is the detection
limit (in grams) for EDX and WDX? Thanks.


####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Neckers Building, Room 146 - B Wing
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: shAf :      mshaf-at-darkwing.uoregon.edu
Date: Mon, 01 Sep 1997 23:26:40 -0700
Subject: Re: detection limits x-ray

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John J. Bozzola wrote:

} ------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} -----------------------------
} -----------------------------------------.
}
} Under ideal (and reasonably attainable conditions), what is the
} detection
} limit (in grams) for EDX and WDX? Thanks.
}
} ...

The question you ask is not specific enough ... to many factors to be
considered with regard to which elements you are interested in, and
(e.g.) ... how sensitive your specimen is to long count times and high
beam currents ... ... ask again ...


cheerios, shAf
--
~~~~~~~~~~~~~~~~~~~~~~~ cogito, ergo zZOooOM ~~~~~~~~~~~~~~~~~~~~
Michael Shaffer - Geological Sciences - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/






From: Keith Moulding :      mcmouldk-at-uxmail.ust.hk
Date: Tue, 02 Sep 1997 15:06:29 +0800
Subject: Electro polishings of Cu,Ni,Al alloy

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Hello,

I need a solution to electro polishing a Cu, Ni, Al alloy (~ 80:14:4 wt%)for
EBSP work. A CrO3 + HClO4 has been suggested, however does anyone know of
any other suitable solutions.

Thanks in advance.

Regards,

Keith.



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Facility
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: Rui Vilar :      pcrvilar-at-alfa.ist.utl.pt
Date: Tue, 02 Sep 1997 10:37:18 +0000
Subject: Post doctoral position available

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Postdoctoral Studentship in Surface Science and Engineering

Applications are invited for Postdoctoral Research Studentships in the
following subjects of study:

- micro and nanotribology
- study of laser processed materials using transmission electron
microscopy.

The Studentships are tenable for one year, renewable for a second year
and include a maintenance grant of about US$20,000 per year. The
Studentship will be supported by a research contract. Applicants should
have a Ph.D. degree in an appropriate subject, or expect to obtain it
before receiving the grant. Experience in transmission electron
microscopy of metallic materials or ceramics is essential.
Formal applications including a CV, names of two referees and
specification of areas of interest and past experience should be sent
to:

Prof. R. Vilar
Departamento de Engenharia de Materiais
Instituto Superior Ticnico
Av. Rovisco Pais, 1096
Lisboa Codex, Portugal
Tel. no. 351-1-8418121, fax no. 351-1-8418121
Email address: pcrvilar-at-alfa.ist.utl.pt.

Closing date is 15 November 1997.




From: DrJohnRuss-at-aol.com
Date: Tue, 2 Sep 1997 07:26:49 -0400 (EDT)
Subject: Announce: Image Proc Tool Kit Updates

Contents Retrieved from Microscopy Listserver Archives
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New additions have been posted at
http://members.aol.com/ImagProcTK/updates.htm for both Mac and Windows users.
The Measure/Size&Shape routine previously available for Mac is now provided
for Windows. Routines to draw grids of lines (horizontal or vertical, or
radial with either uniform or sine weighting) are useful for stereological
counting or to AND with binary images for measurement (both platforms).
Coming next month: Measure/Select for Windows, Color space conversion
routines, and more...

John Russ




From: chris gilpin :      cgilpin-at-fs1.sem.man.ac.uk
Date: Tue, 2 Sep 1997 13:34:57 BST
Subject: Re: Yeast cells, examination using ESEM

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}
}
} Hello all,
} I need to look at the surface morphology of some yeast
} cells and as I'm a physics sort of person I need a little help.
}
} I going to be using an ESEM, but what is the best way to
} prepare yeast cells for this sort of examination?
}
} A step by step answer or reference would be great.
}

Don't prepare the samples!
Mount them on a peltier stage stub, cool them, get the chamber water
vapour stabilised and view them as they are.
For anything more detailed please mail me direct.

Chris


Chris Gilpin
Biological Sciences Electron Microscope Unit
G452 Stopford Building
Oxford Road
Manchester
M13 9PT
phone +44 161 275 5170
fax +44 161 275 5171
http://www.biomed.man.ac.uk/biology/emunit/emhome.html




From: Tobias Baskin :      baskin-at-biosci.mbp.missouri.edu
Date: Tue, 2 Sep 1997 12:13:14 -0600
Subject: amyl alcohol and amyl acetate in the 1950's

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Greetings,
OK, this sounds a bit off topic, but honestly, I encountered this
problem in a procotol for extracting polymerized butyl-methylmethacrylate
resin from sections. The protocol, from the early 1950's, simply calls for
"amyl alcohol" and "amyl acetate". Nowadays, there is a very popular
solvent called "ISOamyl alcohol" (or more formally, 3methyl-1butanol), and
I think this might have been what was being refered to in the fifties as
simply amyl alcohol. Contrarywise, there is a solvent that is still called
in the catalogs "amyl acetate" (more formally, pentacetate), that I guess
is what was meant (despite the fact that a compound exists that is now
called "ISOamyl acetate".
I would like to try this protocol, and I would rather not all of
the different amyl alcohols (n-, t-, dlsec- etc). Can anyone with a long
memory or a good chemistry background help here?
Many thanks!
Tobias Baskin


_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University ofMissouri
/ | / / \ \ \ BiologicalSciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123






From: Edwards, Danny J :      dan.edwards-at-pnl.gov
Date: Tue, 02 Sep 1997 11:32:53 -0700
Subject: RE: Electro polishing of Cu,Ni,Al alloy

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Don't know if this will work for your alloy, but I have used a simple
electrolyte that you can use at room temperature for jet polishing
various copper alloys for TEM. Might work for your purposes.

25% Phosphoric Acid
25% Ethylene Glycol
50% Distilled Water

Good Luck

Dan Edwards


-------------------------------------------------------
Dan Edwards
Structural Materials Research Section
Battelle Pacific Northwest National Laboratory
P.O. Box 999, MSIN P8-15
Richland, WA 99352

Office: 509-376-4867
Fax: 509-376-0418
E-mail: dan.edwards-at-pnl.gov



} -----Original Message-----
} From: Keith Moulding [SMTP:mcmouldk-at-uxmail.ust.hk]
} Sent: Tuesday, September 02, 1997 12:06 AM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Electro polishings of Cu,Ni,Al alloy
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} ----------------------------------------------------------------------
} -.
}
} Hello,
}
} I need a solution to electro polishing a Cu, Ni, Al alloy (~ 80:14:4
} wt%)for
} EBSP work. A CrO3 + HClO4 has been suggested, however does anyone
} know of
} any other suitable solutions.
}
} Thanks in advance.
}
} Regards,
}
} Keith.
}
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr. K. Moulding.
}
} Materials Characterisation and Preparation Facility
} Hong Kong University of Science and Technology,
} Clear Water Bay,
} Kowloon,
} Hong Kong.
}
} FAX: (852) 2358 2451
} TEL: (852) 2358 8724
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Tue, 2 Sep 1997 13:48:37 -0500
Subject: Re: Where to find the NiFeMo alloy phase diagram?

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There is a Fe-Mo-Ni ternary phase diagram in the Journal of Phase
Equilibria 15 (6), 622-626. The article includes references to other
papers.
}
} Dear Sirs:
} I am working on the sputtering NiFe/Mo magnetic multilayers by
} using HREM. The interface between the NiFe and Mo layers may be a kind
} of NiFeMo alloy, but I can't make decision about the idea. So I hope to
} know the NiFeMo phase diagram and the solution degree between NiFe and
} Mo.
}
} I am looking for the help from you and please tell me the
} references where I can find the NiFeMo phase diagram.
}
} Yours Sincerely
}
} Gao Yihua

Russell E. Cook
Scientific Associate
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Building 212
9700 South Cass Avenue
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: J. Mancuso :      amtcorp-at-delphi.com
Date: Tue, 02 Sep 1997 04:25:28 +0000
Subject: Service Engineer Position Available

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AMT has a "fast track" opportunity for a national field service
engineer. The position requires understanding of electron microscopes,
digital technology and software. This individual will be responsible for
installation and support of AMT's imaging and motion control products
for electron microscopy.


Inquiries and questions should be addressed to:

Jim Mancuso
Advanced Microscopy Techniques Corp.
3 Electronics Avenue
Danvers MA 01923

Phone (508)774-5550 Fax (508)739-4313
E-mail: amtcorp-at-delphi.com






From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Tue, 2 Sep 1997 15:29:17 -0500
Subject: Re: amyl alcohol and amyl acetate in the 1950's

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Acording to my material safety data sheets:
Amyl alcohol is 1-pentanol, CAS# 71-41-0, although the name is also
used for a mixture.
Isoamyl alcohol is 3methyl-1butanol, CAS #123-51-3.
Amyl acetate is pentacetate or 1-pentyl acetate or N-pentyl acetate or
N-amyl acetate. CAS# 628-63-7.
Isoamyl acetate is 3methyl-1butyl acetate or isopentyl acetate, CAS#
123-92-2.
}
} Greetings,
} OK, this sounds a bit off topic, but honestly, I encountered this
} problem in a procotol for extracting polymerized butyl-methylmethacrylate
} resin from sections. The protocol, from the early 1950's, simply calls for
} "amyl alcohol" and "amyl acetate". Nowadays, there is a very popular
} solvent called "ISOamyl alcohol" (or more formally, 3methyl-1butanol), and
} I think this might have been what was being refered to in the fifties as
} simply amyl alcohol. Contrarywise, there is a solvent that is still called
} in the catalogs "amyl acetate" (more formally, pentacetate), that I guess
} is what was meant (despite the fact that a compound exists that is now
} called "ISOamyl acetate".
} I would like to try this protocol, and I would rather not all of
} the different amyl alcohols (n-, t-, dlsec- etc). Can anyone with a long
} memory or a good chemistry background help here?
} Many thanks!
} Tobias Baskin
}
}
} _ ____ ^ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ University ofMissouri
} / | / / \ \ \ BiologicalSciences
} /___/ /__ /___ \ \ \__ 109 Tucker Hall
} / / / \ \ \ Columbia, MO 65211-7400 USA
} / / / \ \ \ voice: 573-882-0173
} / /____ / \ \__/ \____ fax: 573-882-0123

Russell E. Cook
Scientific Associate
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Building 212
9700 South Cass Avenue
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: Robert H. Olley :      R.H.Olley-at-reading.ac.uk
Date: Wed, 3 Sep 1997 10:10:57 +0100 (BST)
Subject: Amyl Alcohol : the history

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Traditionally, AMYL ALCOHOL is a mixture of 3-methyl-1-butanol and
2-methyl-1-butanol, derived from the distillation of fermented STARCH
(Latin: amylum). This is almost certainly what was meant in the 1950s and
even much later. These days, you are likely to find this listed as
iso-amyl or iso-pentyl alcohol (or acetate). When catalogues list the
n-isomer they generally specify n-amyl or n-pentyl.

For any traditional protocol, the iso-amyl (or pentyl) is what would have
been on the shelf at the time.

Useful source: "Chemistry of Organic Compounds" by Noller - the 2nd
edition (1957) is particularly good for historical information.

(Incidentally, the higher alcohols are not formed by side-reactions on the
glucose from the starch, but are derived from amino-acids derived from the
yeast or present in the potatoes, or whatever).

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+







From: hilsley :      hilsley-at-chempath.uct.ac.za
Date: Wed, 3 Sep 1997 13:22:57 UTC-2
Subject: hitachi h600 tem

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Hi

I need some advice and wonder if there is any one who can
help me.
We have a Hitachi H-600 tem, the machine is quite old ie. 16 years.
A collegue from a neighbouring centre's, Hitachi h-600 is giving
problems.
They would like to borrow our high voltage board and test it on
their machine to make sure that the board is not broken. I am in a
dilemma as I don't know if what ever caused thei.r problem will damage
our board. I feel I would like to help them out but not if there is a
chance that I might be making problems for myself.
I would really appreciate any advice as I have not been running our
EM lab for very long (2 years), and am still not 100% confident with
the machine.(All our senior staff left and we have been unable to
replace them, so I don't really have anyone to ask who has a lot of
experience with the mechanical running of the Hitachi)

Thanks
Helen Ilsley
Diagnostic EM Lab
UCT Medical School
Groote Schuur Hospital
Cape Town
South Africa
e-mail : hilsley-at-chempath.uct.ac.za




From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 3 Sep 1997 16:55:44 -0400
Subject: RE: Detection limits

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The question of detection limits in X-ray spectroscopic analysis is a
complex one which generally does not have a simple answer. This matter is
discussed in some detail by Heinrich in his book 'Electron Beam X-ray Micro
Analysis', Van Nostrand-Reinhold, 1981 (ISBN 0-442-23286-1) p. 193 & p.
216, with the conclusion that the term 'm inimum detectability limit'
probably should not be used.

The matter is also discussed by Goldstein, et. al. in their book 'Scanning
Electron Microscopy and X-ray Microanalysis', Plenum Prewss,2nd Ed., 1992,
(ISBN 0-306-44175-6). In Table 9.17, p. 501, they give calculated values
comparing MDLs for several different elements for both the EDS and WDS
detection systems. If you don't have this book (you should, however, by
all means obtain a copy if you are doing any work in SEM of X-ray
microanalysis) I can fax you a copy of this discussion.

Best regards,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:313-763-4788; Ph:313-764-3321






From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 3 Sep 1997 15:37:09 -0800
Subject: METHANOL SUBSTITUTE

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Looking for a substitute for methanol for use in electropolishing of metal
samples for TEM observation. Additionally it should have a flash point near or
above 100 deg. F.

Thanks, Mark A. Wall
LLNL





From: Jim Darley :      jim-at-proscitech.com.au
Date: Thu, 4 Sep 1997 15:18:52 +1000
Subject: Re: detection limits x-ray

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Message-Id: {199709040713.RAA17437-at-ultra.ultra.net.au}

Hi John and Michael et al:
I recall that one of your Presidents (Kennedy?) was looking for a one
handed science adviser; the ones he had always prevaricated "on the one
hand and on the other hand".

Michael is quite right, detection limits vary greatly depending on endless
factors. However, it is useful to have some figures as guidelines:
Considering say the 10 elements following sodium. Detection of these is
about best.
For these in EDX the limit for quantitative analysis is about 1%. Detection
limit is about 0.1%. Increasing counts and counting times beyond the
customary 100 seconds at perhaps 2000cps will scarcely improve either
limit.

WDX is near quantitative to its detection limit and that is at least two
orders of magnitude greater than is EDX.

I'll enter correspondence only when it concerns errors in excess of five
orders of magnitude.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au

} John J. Bozzola wrote:
} }
} } Under ideal (and reasonably attainable conditions), what is the
} } detection
} } limit (in grams) for EDX and WDX? Thanks.
}
} The question you ask is not specific enough ... to many factors to be
} considered with regard to which elements you are interested in, and
} (e.g.) ... how sensitive your specimen is to long count times and high
} beam currents ... ... ask again ...
} cheerios, shAf
} --
} ~~~~~~~~~~~~~~~~~~~~~~~ cogito, ergo zZOooOM ~~~~~~~~~~~~~~~~~~~~
} Michael Shaffer - Geological Sciences - University of Oregon
} mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/





From: Jim Darley :      jim-at-proscitech.com.au
Date: Thu, 4 Sep 1997 14:55:45 +1000
Subject: Re: Discarding Uranyl Acetate

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Dear Teresa -
I waited a couple of days but there was no reply on the listserver. Here is
mine:
I do not know about your regulations, maybe you must collect the stuff and
then dispose of it "properly". But too many regulations are not wise - eg.
the dangerous goods shipping laws which appear designed to make things more
expensive but not safer.

Uranium occurs in trace amounts throughout the environment, including
seawater and especially in granite. U does not concentrate in the food
chain like P, K, Pb, Hg or some organic compounds. For very small
quantities, as are used in EM labs, prompt disposal with some water into
the sewage system appears perfectly reasonable to me.
The mistake is to store the stuff and to accumulate larger quantities.

----------
} From: Flores, Teresa {tflore-at-lsumc.edu}
} To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
} Subject:
} Date: Saturday, 30 August 1997 4:33

}
} What do routine labs do with uranyl acetate waste. Ours has been picked
up
} by in house safety department and is being stored in drums as there isn't
a
} company that will pick up to discard. Is anyone else having this problem.
} It is a .025mg uranyl acetate to 100 ml of 50% ethanol washed out with
} copious amounts of sterile distilled water. Any imput will be
appreciated.
} Many thanxs
}
}




From: mtdineen-at-dow.com
Date: Thu, 4 Sep 1997 07:44:16 -0400
Subject: Exporting Tencor Profilometry Data?

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Anyone out there with experience exporting a Tencor P-11 Profilometer 3D
data set to an external analysis program (e.g. IGOR or Nanoscope AFM)?
We are looking for suggestions on successful transfers.


Michael T. Dineen
The Dow Chemical Company
1897 Building
Midland MI 48667
(517/636-4008
4 517/638-6443
+ mtdineen-at-dow.com




From: Ambrose, Wallace :      wambrose.drc-at-mhs.unc.edu
Date: Thu, 4 Sep 1997 8:55:56 -0400
Subject: Denton 502 bell jar

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Hello,
I need to replace the 12 inch bell jar on a Denton 502.
Denton wants $627 for a new one. Does any one know a source
of used bell jars, etc. for this equipment?
Thanks
Wallace Ambrose





From: loakford-at-hsc.unt.edu (Larry Oakford)
Date: Thu, 4 Sep 1997 08:13:28 -0500
Subject: Re: Discarding Uranyl Acetate

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Jim,
U in the USA must be disposed of in a way other than "down the
sewer". It may occur in trace amounts in "nature" but the concentrations
we work with are much higher than that. Besides the legal problems there
are the moral responsibilities to not unnecessarily endanger others with
the chemicals we use. True, uranium is one of the milder toxins we
handle....if handled properly, but it still is a beta emitter and can do
extensive tissue damage if allowed to come into contact with any soft
tissues. Flushing this waste down the sewer does not guarantee that this
will not happen to some unsuspecting individual. That is why there are
regulations in place for the proper handling and disposal of this chemical.
I also empathize with Teresa's dilema because we are in the same
position....radiation waste handlers won't take it because it is a
naturally occurring isotope and hazardous waste handlers won't take it
because it is radioactive....a perfect catch 22. If anyone can come up
with a practical solution to this dilemma that satisfies the current clean
water act regulations we would be more than happy to listen.

Jim Darley wrote:
} Dear Teresa -
} I waited a couple of days but there was no reply on the listserver. Here is
} mine:
} I do not know about your regulations, maybe you must collect the stuff and
} then dispose of it "properly". But too many regulations are not wise - eg.
} the dangerous goods shipping laws which appear designed to make things more
} expensive but not safer.
}
} Uranium occurs in trace amounts throughout the environment, including
} seawater and especially in granite. U does not concentrate in the food
} chain like P, K, Pb, Hg or some organic compounds. For very small
} quantities, as are used in EM labs, prompt disposal with some water into
} the sewage system appears perfectly reasonable to me.
} The mistake is to store the stuff and to accumulate larger quantities.
}
} ----------
} } From: Flores, Teresa {tflore-at-lsumc.edu}
} } To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
} } Subject:
} } Date: Saturday, 30 August 1997 4:33
}
} }
} } What do routine labs do with uranyl acetate waste. Ours has been picked
} up
} } by in house safety department and is being stored in drums as there isn't
} a
} } company that will pick up to discard. Is anyone else having this problem.
} } It is a .025mg uranyl acetate to 100 ml of 50% ethanol washed out with
} } copious amounts of sterile distilled water. Any imput will be
} appreciated.
} } Many thanxs
} }
} }

XXX
XXX
XXX
XXX
[]-XXX---
XXX
XXX
-------XXX--------
| o XOX [] |
-------XXX--------
| |
| |
------------------

Lawrence X. Oakford, Ph.D.
Department of Anatomy and Cell Biology
UNT Health Science Center
Fort Worth, TX 76107
e-mail:xavier-at-jove.acs.unt.edu






From: jeharper-at-amoco.com
Date: 9/4/97 12:18 AM
Subject: Re: detection limits x-ray

Contents Retrieved from Microscopy Listserver Archives
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I agree, EDX detection limit somewhere just less than 1%...but

I work with polymer fibers and by ashing I can get great analysis of
additives at 100 ppm in the polymer. This doesn't change the EDX
detection limit per se, but there is more than one way to skin a cat.

My apologies to all cat lovers.

Jim Harper
Amoco Fabrics and Fibers


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Hi John and Michael et al:
I recall that one of your Presidents (Kennedy?) was looking for a one
handed science adviser; the ones he had always prevaricated "on the one
hand and on the other hand".

Michael is quite right, detection limits vary greatly depending on endless
factors. However, it is useful to have some figures as guidelines:
Considering say the 10 elements following sodium. Detection of these is
about best.
For these in EDX the limit for quantitative analysis is about 1%. Detection
limit is about 0.1%. Increasing counts and counting times beyond the
customary 100 seconds at perhaps 2000cps will scarcely improve either limit.

WDX is near quantitative to its detection limit and that is at least two
orders of magnitude greater than is EDX.

I'll enter correspondence only when it concerns errors in excess of five
orders of magnitude.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au

} John J. Bozzola wrote:
} }
} } Under ideal (and reasonably attainable conditions), what is the
} } detection
} } limit (in grams) for EDX and WDX? Thanks.
}
} The question you ask is not specific enough ... to many factors to be
} considered with regard to which elements you are interested in, and
} (e.g.) ... how sensitive your specimen is to long count times and high
} beam currents ... ... ask again ...
} cheerios, shAf
} --
} ~~~~~~~~~~~~~~~~~~~~~~~ cogito, ergo zZOooOM ~~~~~~~~~~~~~~~~~~~~
} Michael Shaffer - Geological Sciences - University of Oregon
} mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/





From: Franklin Bailey :      jfb-at-novell.uidaho.edu
Date: Thu, 4 Sep 1997 08:48:00 PST
Subject: Zeiss EM-10 info

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a set of circuit diagrams for a Zeiss EM-10A
transmission electron microscope. If anyone is willing to part with
an old set, or will allow me to copy a set, please email me at:

jfb-at-uidaho.edu

Thank you.

Franklin Bailey




From: John Arnott :      ladres-at-worldnet.att.net
Date: Thu, 04 Sep 1997 12:14:18 -0400
Subject: request for help

Contents Retrieved from Microscopy Listserver Archives
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To All,

One of our customers has asked for some advice. He's picking up 1 inch
diameter, .040" thick aluminum discs and exposing them to a chemical
process which requires the least amount of the disc to be obstructed. He
now uses triceps to hold the discs but they lose their memory after a
mumber of uses.
Can anyone suggest a better way to handle the discs?

Thanks,
John Arnott
Ladd Research
ladres-at-worldnet.att.net




From: Jim Darley
Date: Thursday, September 04, 1997 6:58AM
Subject: Re: Discarding Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
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Teresa,
I was hoping that someone experianced in haz. waste disposal methods would
comment on your question.
In my experiance, the problem with UAC waste disposal is if the
radioactive material is in methanol. I have no trouble having the waste
picked up if the UAC is in water. I have been told by Environmental health
and Safety personnel that no disposal option exists for the UAC in methanol.
You have brought up a very important topic and hopefully comments from
other labs and especially from individuals experianced in waste disposal may
follow.

Robert Cox
Shriner Hospital
Galveston Tx.
--------
-----------------------------------------------------------------------.

Dear Teresa -
I waited a couple of days but there was no reply on the listserver. Here is
mine:
I do not know about your regulations, maybe you must collect the stuff and
then dispose of it "properly". But too many regulations are not wise - eg.
the dangerous goods shipping laws which appear designed to make things more
expensive but not safer.

Uranium occurs in trace amounts throughout the environment, including
seawater and especially in granite. U does not concentrate in the food
chain like P, K, Pb, Hg or some organic compounds. For very small
quantities, as are used in EM labs, prompt disposal with some water into
the sewage system appears perfectly reasonable to me.
The mistake is to store the stuff and to accumulate larger quantities.

----------
} From: Flores, Teresa {tflore-at-lsumc.edu}
} To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
} Subject:
} Date: Saturday, 30 August 1997 4:33

}
} What do routine labs do with uranyl acetate waste. Ours has been picked
up
} by in house safety department and is being stored in drums as there isn't
a
} company that will pick up to discard. Is anyone else having this problem.
} It is a .025mg uranyl acetate to 100 ml of 50% ethanol washed out with
} copious amounts of sterile distilled water. Any imput will be
appreciated.
} Many thanxs
}
}




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Thu, 4 Sep 1997 11:35:58 -0600
Subject: Re: Discarding Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The problem of Ur disposal will continue to exist until someone develops a
plan on how to dispose of the material AND makes a decision that is legally
binding. One way to start the ball rolling is to formulate some possible
ways of dealing with the material and then to forward the package to
appropriate legislators.

Here is what I propose: take some epoxy resin and coat the inside of a
polypropylene beaker with the epoxy by building up layers and polymerizing
the layers. If one uses outdated, quite viscous epoxy monomers, one can
achieve several mm of thickness in a couple days. Now, use the epoxy coated
vessel as a waste container into which you pour your uranium containing
liquids. Keep the vessel in a warm oven or fume hood (if volatiles are
involved) to speed up evaporation. Allow the uranium liquids to evaporate
to dryness (taking care to avoid generating dust) and apply another layer
of epoxy over the uranium salts. What one gets is a layered system of
epoxy/uranium/epoxy/uranium etc. Keep this up until the polypropylene
vessel is completely filled with epoxy. The enrobed uranium can then be
disposed in a toxic waste burial site where it might be further enrobed.

If users scale back on the use of uranium using tiny amounts and minimize
rinse volumes, some of the problem will already be solved.

Key ingredients: scale down use, encapsulate dried salts in epoxy.


####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Neckers Building, Room 146 - B Wing
Southern Illinois University
Carbondale, IL 62901
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: Marti, Jordi :      MartiJ-at-MTOMP201.Research.Allied.com
Date: Thu, 04 Sep 97 13:50:00 EDT
Subject: RE: Denton 502 bell jar

Contents Retrieved from Microscopy Listserver Archives
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Wallace:

Are you replacing the bell jar because the rim is chipped ?. If so have you
thought of having the rim cut down to remove the damaged area ? We had
that done several times by a glass blower at a fraction of the cost of a
new bell jar.

Jordi Marti
-----------------------
Hello,
I need to replace the 12 inch bell jar on a Denton 502.
Denton wants $627 for a new one. Does any one know a source
of used bell jars, etc. for this equipment?
Thanks
Wallace Ambrose





From: oshel-at-staff.uiuc.edu (Philip Oshel)
Date: Thu, 4 Sep 1997 12:59:33 -0600
Subject: Re: request for help

Contents Retrieved from Microscopy Listserver Archives
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How about a trivet, like metalsmiths use for enamelling? (Check the metals
program at your nearest college's art dept.) The tips can be modified by a
little judicious filing to minimize the areas of contact between the disc
and the legs of the trivet.

Phil

} To All,
}
} One of our customers has asked for some advice. He's picking up 1 inch
} diameter, .040" thick aluminum discs and exposing them to a chemical
} process which requires the least amount of the disc to be obstructed. He
} now uses triceps to hold the discs but they lose their memory after a
} mumber of uses.
} Can anyone suggest a better way to handle the discs?
}
} Thanks,
} John Arnott
} Ladd Research
} ladres-at-worldnet.att.net

}}}}}}}}}}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{{{{{{{{{{

Philip Oshel
Station A
PO Box 5037
Champaign, IL 61825-5037
(217) 355-1143
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again *****







From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 4 Sep 1997 14:28:10 -0400
Subject: RE: Denton 502 bell jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

VWR lists a 304 x 304 mm diameter jar for vacuum work for $409.80 US.
It has a lip so I don't know if it will accept a standard L-gasket.

Catalog No.: 14150-001
http://www.vwrsp.com
(800) 932-5000

I have no financial interest in VWR, etc....
}
}
} Harold J. Crossman
} OSRAM SYLVANIA INC.
} Lighting Research Center
} 71 Cherry Hill Dr.
} Beverly, MA 01915
} Phone: (508) 750-1717
} E-mail: crossman-at-osi.sylvania.com
}
} Our web sites: www.sylvania.com
} www.siemens.com
} --
}
} "Crossman, Harold" {crossman-at-osi.SYLVANIA.com}




From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 4 Sep 1997 14:28:10 -0400
Subject: RE: Denton 502 bell jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

VWR lists a 304 x 304 mm diameter jar for vacuum work for $409.80 US.
It has a lip so I don't know if it will accept a standard L-gasket.

Catalog No.: 14150-001
http://www.vwrsp.com
(800) 932-5000

I have no financial interest in VWR, etc....
}
}
} Harold J. Crossman
} OSRAM SYLVANIA INC.
} Lighting Research Center
} 71 Cherry Hill Dr.
} Beverly, MA 01915
} Phone: (508) 750-1717
} E-mail: crossman-at-osi.sylvania.com
}
} Our web sites: www.sylvania.com
} www.siemens.com
} --
}
} "Crossman, Harold" {crossman-at-osi.SYLVANIA.com}




From: loakford-at-hsc.unt.edu (Larry Oakford)
Date: Thu, 4 Sep 1997 13:39:47 -0500
Subject: Re: Discarding Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Uranium in the USA must be disposed of in a way other than "down
the sewer". It may occur in trace amounts in "nature" but the
concentrations we work with are much higher than that. Besides the legal
problems there are the moral responsibilities to not unnecessarily endanger
others with the chemicals we use. True, uranium is one of the milder
toxins we handle....if handled properly, but it still is a beta emitter and
can do extensive tissue damage if allowed to come into contact with any
soft tissues. Flushing this waste down the sewer does not guarantee that
this will not happen to some unsuspecting individual. That is why there
are regulations in place for the proper handling and disposal of this
chemical.
I also empathize with Teresa's dilema because we are in the same
position....radiation waste handlers won't take it because it is a
naturally occurring isotope and hazardous waste handlers won't take it
because it is radioactive....a perfect catch 22. If anyone can come up
with a practical solution to this dilemma that satisfies the current clean
water act regulations we would be more than happy to listen.

Jim Darley wrote:
} Dear Teresa -
} I waited a couple of days but there was no reply on the listserver. Here is
} mine:
} I do not know about your regulations, maybe you must collect the stuff and
} then dispose of it "properly". But too many regulations are not wise - eg.
} the dangerous goods shipping laws which appear designed to make things more
} expensive but not safer.
}
} Uranium occurs in trace amounts throughout the environment, including
} seawater and especially in granite. U does not concentrate in the food
} chain like P, K, Pb, Hg or some organic compounds. For very small
} quantities, as are used in EM labs, prompt disposal with some water into
} the sewage system appears perfectly reasonable to me.
} The mistake is to store the stuff and to accumulate larger quantities.
}
} ----------
} } From: Flores, Teresa {tflore-at-lsumc.edu}
} } To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
} } Subject:
} } Date: Saturday, 30 August 1997 4:33
}
} }
} } What do routine labs do with uranyl acetate waste. Ours has been picked
} up
} } by in house safety department and is being stored in drums as there isn't
} a
} } company that will pick up to discard. Is anyone else having this problem.
} } It is a .025mg uranyl acetate to 100 ml of 50% ethanol washed out with
} } copious amounts of sterile distilled water. Any imput will be
} appreciated.
} } Many thanxs
} }
} }

XXX
XXX
XXX
XXX
[]-XXX---
XXX
XXX
-------XXX--------
| o XOX [] |
-------XXX--------
| |
| |
------------------

Lawrence X. Oakford, Ph.D.
Department of Anatomy and Cell Biology
UNT Health Science Center
Fort Worth, TX 76107
e-mail:xavier-at-jove.acs.unt.edu






From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 4 Sep 1997 15:17:43 -0400
Subject: RE: Replacement bell jars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Duniway Stockroom Corp., 1305 Space Park Way, Mountain View, CA 94043,
Tel. 800-446-8811, e.mail: info-at-duniway.com, deals in new, used, and
rebuilt vacuum equipment. You might copntact them.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:313-763-4788; Ph:313-764-3321






From: Paul VANDERLINDEN :      orion-at-euronet.be
Date: Thu, 4 Sep 1997 21:40:02 +0200 (MET DST)
Subject: (Fwd) (Fwd) Virus Warning (fwd) -RETRANSMIS

Contents Retrieved from Microscopy Listserver Archives
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} Date: Thu, 04 Sep 1997 14:11:03 +0200
} From: Catherine Goffaux {catherine.goffaux-at-wkb.be}
} To: njoschko-at-datagram.be, claudejp-at-esvax.dnet.dupont.com, orion-at-infoboard.be,
} deschuyt-at-sbbio.be, bdd.translations-at-skynet.be, labio-at-telecom-plus.sn,
} sandra.rens-at-vulcan.be
} Subject: (Fwd) (Fwd) Virus Warning (fwd) -RETRANSMIS
}
} Received: from mserv.rug.ac.be by wkb.be (SMI-8.6/SMI-SVR4)
} id NAA01015; Sun, 31 Aug 1997 13:12:47 +0200
} Received: from eduserv2.rug.ac.be by mserv.rug.ac.be with SMTP id AA14051
} (5.67b/IDA-1.5 for {inge.vanderhaegen-at-wkb.be} ); Sun, 31 Aug 1997 13:21:01
+0200
} Received: from localhost by eduserv2.rug.ac.be (SMI-8.6/SMI-SVR4)
} id NAA03840; Sun, 31 Aug 1997 13:20:59 +0200
} Message-Id: {Pine.SOL.3.94.970831131452.3715A-100000-at-eduserv2.rug.ac.be}
} Date: Sun, 31 Aug 1997 13:20:59 +0200
} From: Kristof Van der Haegen {Kristof.VanderHaegen-at-rug.ac.be}
} To: Karl.Theeten-at-rug.ac.be
} Cc: Peter.Forret-at-keyware.be,Bartel.Van.Der.Haegen-at-mobile.belgacom.be,
} Benedikt.Ameloot-at-rug.ac.be, Gunther.Heene-at-rug.ac.be,
} Luc.VanSintJan-at-rug.ac.be, Samuel.VanBelle-at-rug.ac.be,
} inge.vanderhaegen-at-wkb.be
} Subject: (Fwd) (Fwd) Virus Warning (fwd)
} Mime-Version: 1.0
} Content-Type: text/plain
} Content-Disposition: inline
}
} PLEASE read the following warning.
} WARNING!!!!!! If you receive an e-mail titled "JOIN THE CREW"
} DO NOT open it!
} It will erase EVERYTHING on your hard drive! Send this letter out to
} as many people you can.......this is a new virus and not many people
} know about it!
}
} This information was received this morning from IBM, please share it
} with anyone that might access the Internet.
}
} Also,
}
} If anyone receives mail entitled; PENPAL GREETINGS! please delete it
} WITHOUT reading it!! This is a warning for all Internet users -
} there is a dangerous virus propagating across the Internet through an
} e-mail message entitled "PENPAL GREETINGS!".
}
} DO NOT DOWNLOAD ANY MESSAGE ENTITLED "PENPAL GREETINGS"!!
}
} This message appears to be a friendly letter asking you if you are
} interested in a penpal, but by the time you read this letter, it is
} too late. The trojan horse" virus will have already infected the boot
} sector
} of your hard drive, destroying all of the data present. It is a
} self-replicating virus, and once the message is read, it will
} AUTOMATICALLY forward itself to anyone who's e-mail address is present
} in YOUR mailbox!
}
} This virus will DESTROY your hard drive, and holds the potential to
} DESTROY the hard drive of anyone whose mail is in your in box, and
} who's
} mail is in their in box and so on. If this virus keeps getting
} passed, it has the potential to do a great deal of DAMAGE to computer
} networks worldwide!!!!
}
} Please, delete the message entitled "PENPAL GREETINGS!" as soon as you
} see it! And pass this message along to all of your friends, relatives
} and the other readers of the newsgroups and mailing lists which you
} are on so that they are not hurt by this dangerous virus!!!!
}
} Please pass this along to everyone you know so this can be stopped.
} PASS THIS ON TO YOUR FRIENDS!!! WARNING !!!
} There is a new virus going around in the last couple of days!!!
} DO NOT open or even look at any mail that you get that says: "Returned
} or Unable to Deliver" This virus will attach itself to your computer
} components and render them useless. Immediately delete any mail items
} that says this. AOL has said this is a very dangerous virus, and there
} is NO remedy for it at this time, Please Be Careful, And forward to
} all your on-line friends A.S.A.P.
}
} Forward this A.S.A.P. to every single person you know!!!!!!!!!
} ***************************************************
}
}
}
}


Best regards,



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Sales Manager.

=======================================================================
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From: Anthony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Thu, 04 Sep 1997 16:21:30 -0400
Subject: Re: detection limits x-ray

Contents Retrieved from Microscopy Listserver Archives
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Jim Darley's posting is hardly furthering good science.

1: Detection limits (John's original question). Modern EDX systems are
easily capable of quantitative analysis in the sub-1wt% range. See, for
example http://prism.mit.edu/facltis/stem/stmexam.htm (the second
illustration on that page) where I was getting analyses for Cr in steel of
the order of 0.6+/-0.1 wt%. I don't know about you, but I consider this
quantitative. This, mind you, was in a measurement where I was attempting
to optimize spatial resolution rather than sensitivity, and the acquisition
time was 60sec. per data point. This example, of course, was from a STEM.
There are many more examples in the literature.

Using beam gating techniques such as those developed by Charlie Lyman and
colleagues, EDX data can readily be acquired at 20,000-40,000 counts per
real second, if spatial resolution is sacrificed. Combine this with an
acquisition time of 1,000 seconds (by no means unrealistic for an important
measurement) and the detection limit is in the rage of, or better than, 0.01wt%.

Of course, in the SEM, which may have been the point of John's original
question, the situation is not the same, the beam voltage is lower
(resulting in poorer peak/bremmstrahlung ratios) and the emission of x-rays
from a solid sample is different from that in a thin foil, but these are
some of the variables that Michael quite rightly pointed out must be considered.

2: Comparison between EDX and WDX.

This is like comparing apples and oranges, because the instruments designed
with them are generally intended for different purposes.

WDX has a much better peak resolution than WDX, which results in better
measured P/B ratios (and hence improved statistics), as well as much better
capability in resolving nearby x-ray lines. Also, because the x-ray
counting and wavelength analysis are different functions in the crystal
spectrometer, available countrates have traditionally been higher in WDX
than EDX (although modern EDX detectors are an order of magnitude faster
than they were fifteen years ago). Against this must be set the fact that
WDX is inherently a serial technique (although multiple spectrometers help
here), while EDX is a parallel technique (compare the advantages of PEELS
over SEELS - although this is not a totally fair comparison). Anyway, the
advantage of WDX (ignoring the capability of resolving peak overlaps) is
improved statistical precision. Is this useful?

Well, maybe.

By far the most significant parameter which affects electron-induced x-ray
emission spectra is sample geometry. Two extreme cases are where the sample
is a thin foil (as in the STEM) where, to a first approximation, the x-ray
spectrum recorded by the detector is the same as that emitted, and to a
second order, it is possible to derive a reasonable thickness correction for
cases where the error is small. Alternatively, when the sample has
dimensions large compared with the volume irradiated by the electron beam,
and has an accurately known geometry compared to the incident beam and the
detector (such as a polished flat sample in the microprobe), correction
programs such as ZAF can do a reasonable job of extracting a quantitative
analysis.

What about where the sample geometry is unknown (for example, a rough
surface such as might be examined in the SEM)? In that case the uncertainty
in the analysis is far, far worse than any uncertainty caused by the poor
statistics of the EDX spectrum, so there is no point or advantage whatever
in using WDX to try to improve things, because it won't. This is why
typically an SEM has an EDX detector - it is cheaper and gives just as good
an analysis (except for the somewhat poorer detection limit). In the
microprobe, great care is taken in polishing and mounting the sample, so the
advantage of the WDX detector can be realised.

Where does this leave us? The advantage of a WDX detector over an EDX
detector *ON THE SAME SAMPLE* is limited - perhaps an order of magnitude in
detection limit, and, on a flat, polished sample, also perhaps approaching
an order of magnitude in precision. The WDX detector can also resolve many
cases where peaks would overlap in EDX. On general rough SEM samples, the
only advantages of WDX are a small improvement in detection limits and the
ability to resolve overlaps (which could be important if a trace element
peak overlaps a major peak in the EXD spectrum.

The President's science advisors were right - it all depends. I seem to
remember that one of Jim Darley's Prime Ministers (Gough Whitlam?) had to be
dismissed because Parliament would not pass his budget - but does that have
anything to do with Science?

Tony Garratt-Reed


} Hi John and Michael et al:
} I recall that one of your Presidents (Kennedy?) was looking for a one
} handed science adviser; the ones he had always prevaricated "on the one
} hand and on the other hand".
}
} Michael is quite right, detection limits vary greatly depending on endless
} factors. However, it is useful to have some figures as guidelines:
} Considering say the 10 elements following sodium. Detection of these is
} about best.
} For these in EDX the limit for quantitative analysis is about 1%. Detection
} limit is about 0.1%. Increasing counts and counting times beyond the
} customary 100 seconds at perhaps 2000cps will scarcely improve either
} limit.
}
} WDX is near quantitative to its detection limit and that is at least two
} orders of magnitude greater than is EDX.
}
} I'll enter correspondence only when it concerns errors in excess of five
} orders of magnitude.
} Cheers
} Jim Darley
}
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Phone +61 77 740 370 Fax: +61 77 892 313
} Great microscopy catalogue, 400+ Links, MSDS
} ************************ http://www.proscitech.com.au
}
} } John J. Bozzola wrote:
} } }
} } } Under ideal (and reasonably attainable conditions), what is the
} } } detection
} } } limit (in grams) for EDX and WDX? Thanks.
} }
} } The question you ask is not specific enough ... to many factors to be
} } considered with regard to which elements you are interested in, and
} } (e.g.) ... how sensitive your specimen is to long count times and high
} } beam currents ... ... ask again ...
} } cheerios, shAf
} } --
}


Anthony J. Garratt-Reed
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
United States of America

Ph: 617-253-4622
Fax: 617-258-6478





From: Lucille A. Giannuzzi :      lag-at-pegasus.cc.ucf.edu
Date: Thu, 4 Sep 1997 16:24:09 -0400
Subject: JEOL 6400 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We received an JEOL 6400 SEM without an instruction manual. If anyone can
help us out with a copy of a manual, we'd greatly appreciate it!


*************************************************************************
Lucille A. Giannuzzi, Ph.D.

Assistant Professor
Dept. of Mechanical, Materials, and Aerospace Eng.

Director, Cirent/UCF Materials Characterization Facility
President, Florida Microscopy Society (a local affiliate of MSA)

University of Central Florida phone (407) 823-5770
PO Box 162450 fax (407) 823-0208
4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
Orlando, FL 32816-2450 USA
*************************************************************************







From: Craig, Bob :      craig-at-OSI.SYLVANIA.com
Date: Thu, 4 Sep 1997 16:26:52 -0400
Subject: Virus Warnings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings all,

Before sending chills of panic up and down the spines of all of us
computer freaks by posting information regarding an alleged virus one
might check the latest information compiled by the Department of Energy
at their web site dedicated to computer security.

The URL is http://ciac.llnl.gov/

The "viruses" listed are hoaxes according to CIAC.

Bob Craig
OSRAM SYLVANIA Products Inc.
Beverly, MA 01915




From: Barr, Dennis B :      dennbarr-at-eastman.com
Date: Thu, 4 Sep 1997 17:11:54 -0400
Subject: RE: (Fwd) (Fwd) Virus Warning (fwd) -RETRANSMIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul Vanderlinden's posting included warnings about some computer
viruses. While computer viruses can create many problems for the person
or organization where an infection occurs, computer virus HOAXES can
create their own problems as well.

According to the DOE, the PENPAL GREETINGS virus is a hoax, and possibly
the other ones mentioned in Paul Vanderlinden's posting are also.
Information about viruses can be found at the U.S. Department of Energy
Computer Incident Advisory Capability (DOE-CIAC) web site which is at
http://ciac.llnl.gov/
and the hoaxes page at
http://ciac.llnl.gov/ciac/CIACHoaxes.html

Anyone who receives warnings about computer viruses should follow the
DOE's recommended action...

"Users are requested to please not spread unconfirmed warnings about
viruses and Trojans. If you receive an unvalidated warning, don't pass
it to all your friends, pass it to your computer security manager to
validate first. Validated warnings from the incident response teams and
antivirus vendors have valid return addresses and are usually PGP signed
with the organization's key." (from the DOE-CIAC web page on Internet
Hoaxes)






From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 4 Sep 1997 14:36:11 -0700
Subject: Re: (Fwd) (Fwd) Virus Warnin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Try a vacuum forceps. They are available from all microscopy supply =
houses and places like Edmund scientific etc. It is a pen like implement =
which one puts various size needles on depending on the size one wants to =
pick up. Suction is created by a very small motor. No microscopy lab =
should be without one.

Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/954-5284
FAX: 209/954-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html

__________________________________________________________________________=
_____

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To All,

One of our customers has asked for some advice. He's picking up 1 inch
diameter, .040" thick aluminum discs and exposing them to a chemical
process which requires the least amount of the disc to be obstructed. He
now uses triceps to hold the discs but they lose their memory after a
mumber of uses.
Can anyone suggest a better way to handle the discs?

Thanks,
John Arnott
Ladd Research
ladres-at-worldnet.att.net

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Message-ID: {340EDE5A.6A22-at-worldnet.att.net}
"Paul VANDERLINDEN" {orion-at-euronet.be}
X-Mailer: Mail*Link SMTP/QM 3.0.0



From: Paul VANDERLINDEN
Date: 9/4/97 12:56 PM
Subject: Re: (Fwd) (Fwd) Virus Warnin

Contents Retrieved from Microscopy Listserver Archives
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Reply to: RE} (Fwd) (Fwd) Virus Warning (fwd) -RETRANSMIS

See http://ciac.llnl.gov/ciac/CIACHoaxes.html
for information on the PenPal hoax.

-Mike
--------------------------------------

} Date: Thu, 04 Sep 1997 14:11:03 +0200
} From: Catherine Goffaux {catherine.goffaux-at-wkb.be}
} To: njoschko-at-datagram.be, claudejp-at-esvax.dnet.dupont.com,
orion-at-infoboard.be,
} deschuyt-at-sbbio.be, bdd.translations-at-skynet.be,
labio-at-telecom-plus.sn,
} sandra.rens-at-vulcan.be
} Subject: (Fwd) (Fwd) Virus Warning (fwd) -RETRANSMIS
}
} Received: from mserv.rug.ac.be by wkb.be (SMI-8.6/SMI-SVR4)
} id NAA01015; Sun, 31 Aug 1997 13:12:47 +0200
} Received: from eduserv2.rug.ac.be by mserv.rug.ac.be with SMTP id AA14051
} (5.67b/IDA-1.5 for {inge.vanderhaegen-at-wkb.be} ); Sun, 31 Aug 1997 13:21:01
+0200
} Received: from localhost by eduserv2.rug.ac.be (SMI-8.6/SMI-SVR4)
} id NAA03840; Sun, 31 Aug 1997 13:20:59 +0200
} Message-Id: {Pine.SOL.3.94.970831131452.3715A-100000-at-eduserv2.rug.ac.be}
} Date: Sun, 31 Aug 1997 13:20:59 +0200
} From: Kristof Van der Haegen {Kristof.VanderHaegen-at-rug.ac.be}
} To: Karl.Theeten-at-rug.ac.be
} Cc: Peter.Forret-at-keyware.be,Bartel.Van.Der.Haegen-at-mobile.belgacom.be,
} Benedikt.Ameloot-at-rug.ac.be, Gunther.Heene-at-rug.ac.be,
} Luc.VanSintJan-at-rug.ac.be, Samuel.VanBelle-at-rug.ac.be,
} inge.vanderhaegen-at-wkb.be
} Subject: (Fwd) (Fwd) Virus Warning (fwd)
} Mime-Version: 1.0
} Content-Type: text/plain
} Content-Disposition: inline
}
} PLEASE read the following warning.
} WARNING!!!!!! If you receive an e-mail titled "JOIN THE CREW"
} DO NOT open it!
} It will erase EVERYTHING on your hard drive! Send this letter out to
} as many people you can.......this is a new virus and not many people
} know about it!
}
} This information was received this morning from IBM, please share it
} with anyone that might access the Internet.
}
} Also,
}
} If anyone receives mail entitled; PENPAL GREETINGS! please delete it
} WITHOUT reading it!! This is a warning for all Internet users -
} there is a dangerous virus propagating across the Internet through an
} e-mail message entitled "PENPAL GREETINGS!".
}
} DO NOT DOWNLOAD ANY MESSAGE ENTITLED "PENPAL GREETINGS"!!
}
} This message appears to be a friendly letter asking you if you are
} interested in a penpal, but by the time you read this letter, it is
} too late. The trojan horse" virus will have already infected the boot
} sector
} of your hard drive, destroying all of the data present. It is a
} self-replicating virus, and once the message is read, it will
} AUTOMATICALLY forward itself to anyone who's e-mail address is present
} in YOUR mailbox!
}
} This virus will DESTROY your hard drive, and holds the potential to
} DESTROY the hard drive of anyone whose mail is in your in box, and
} who's
} mail is in their in box and so on. If this virus keeps getting
} passed, it has the potential to do a great deal of DAMAGE to computer
} networks worldwide!!!!
}
} Please, delete the message entitled "PENPAL GREETINGS!" as soon as you
} see it! And pass this message along to all of your friends, relatives
} and the other readers of the newsgroups and mailing lists which you
} are on so that they are not hurt by this dangerous virus!!!!
}
} Please pass this along to everyone you know so this can be stopped.
} PASS THIS ON TO YOUR FRIENDS!!! WARNING !!!
} There is a new virus going around in the last couple of days!!!
} DO NOT open or even look at any mail that you get that says: "Returned
} or Unable to Deliver" This virus will attach itself to your computer
} components and render them useless. Immediately delete any mail items
} that says this. AOL has said this is a very dangerous virus, and there
} is NO remedy for it at this time, Please Be Careful, And forward to
} all your on-line friends A.S.A.P.
}
} Forward this A.S.A.P. to every single person you know!!!!!!!!!
} ***************************************************
}
}
}
}


Best regards,



Paul Vanderlinden.
Sales Manager.

=======================================================================
See our web site: http://www.microscopy-uk.org.uk

To contact us:

E.L.I. sprl

Technical support:
Jean-Louis Leclef: Phone: +32 67 21 25 07
Fax : +32 67 22 09 53
Email: oriontech-at-euronet.be
Sales support:
Paul Vanderlinden: Phone: +32 2 726 31 02
Fax : +32 2 726 08 65
Email: orion-at-euronet.be
=======================================================================









From: Barbara Foster :      mme-at-map.com
Date: Thu, 04 Sep 1997 18:07:36 -0700
Subject: Course Reminder: "Optimizing Light Microscopy"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {340F5B58.27E4-at-map.com}

Course Announcement: "Optimizing Light Microscopy"
When/Where:
(a) New York City, November 3,1997
(b) Springfield, MA November 5, 1997
(c) Boston, MA November 7,1997
What: a lively, fast-paced slide lecture and demonstraton for anyone how
uses or plans to use a light microscope: students, teachers, medical
technologists, clinicians, pathologists, and lab managers. Beginning to
more experienced practictioners welcome.

For details...
(a) read below
(b) send for brochure
(c) visit the Microscopy/Microscopy Education booth at
MSA - #502

Program:
1. A quick tour around the microscope - getting to know the bits & pieces
2. Koehler illumination & you: 4 critical steps for aligning you and your
microscope to reduce headaches, fatigue, and errors
3. Care and cleaning
4. Useful principles for understanding and optimizing imaging
5. Putting the basics to work:
a. Troubleshooting
b. Understanding Phase and Hoffman Modulation Contrast
6. The Video connection: cameras, computers, and your microscope
7. Bringing out the best: quick, easy, and often free techniques for
improving contrast
8. Advanced contrast techniques: Fluorescence and DIC
9. Becoming a better consumer: matching your microscope to your
application
10. Questions, Answers, and Information Exchange
(Note: Instructor may vary class content slightly to meet the needs of
participants)

Free with your tuition: "Optimizing Light Microscopy for Biological and
Clinical Laboratories" (Kendall-Hunt, 1997). Nearly 200 pages of helpful
hints, quick experiments, and new iedas for getting the best from your
microscope. Hot off the presses!

CEU's: 0.6 CEUs, 6 P.A.C.E CEU's
Microscopy/Microscopy Education adheres to the guidelines
established by the IACET.

Pricing:
$150 (includes tuition, breaks, course materials, and copy of book)
*****Save $25 if paid by 10/17/97.*****
Send three from the same facility and save $50 on tuition for the third
person.

Refund policy:
Full refund for cancellations made by 10/17/97. After that date, 50%
refund or full credit for future class. Substitutions accepted.

Questions: call Barbara Foster or Dr. Ken Piel at MME: (413)746-6931

Registration: Download the form below and fax to (413)746-9311 or call
(413)746-6931 and ask for Ken.

Check course you will be attending:
___ New York City, November 3 (#971103)
___ Springfield, November 5 (#971105)*
___ Boston, November 7 (#971107)

*Catered lunch available for extra $15.00

Name: _______________________________________________________________
School/Hospital/Company: ____________________________________________
Address: ____________________________________________________________
City/State/Zip: _____________________________________________________
Phone: ______________________________________________________________
Fax: ________________________________________________________________
Email: ______________________________________________________________

Method of Payment:
____ Check enclosed for $ _______________
____ Visa
____ Mastercard
Name on credit card: __________________________________
Credit card number: ___________________________________
Expiration date: ______________________________________
***If billing address is different from one shown above,
please show billing address below:
_______________________________________________________
_______________________________________________________
_______________________________________________________




From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 4 Sep 1997 15:05:38 -0700
Subject: Hoaxes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From --
http://ciac.llnl.gov/ciac/CIACHoaxes.html

**************************************************************************
PENPAL GREETINGS! Warning Hoax

The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an
e-mail chain letter by claiming that it is a self starting Trojan that
destroys your hard drive and then sends copies of itself to everyone whose
address in in your mailbox. Reading an e-mail message does not run it nor
does it run any attachments, so this Trojan must be self starting. Aside from
the fact that a program cannot start itself, the Trojan would also have to
know about every different kind of e-mail program to be able to forward
copies of itself to other people. This warning is totally a hoax.

FYI!

Subject: Virus Alert
Importance: High
If anyone receives mail entitled: PENPAL GREETINGS! please delete it
WITHOUT
reading it. Below is a little explanation of the message, and what it
would
do to your PC if you were to read the message. If you have any
questions or
concerns please contact SAF-IA Info Office on 697-5059.

This is a warning for all internet users - there is a dangerous virus
propogating across the internet through an e-mail message entitled
"PENPAL
GREETINGS!".
DO NOT DOWNLOAD ANY MESSAGE ENTITLED "PENPAL GREETINGS!"
This message appears to be a friendly letter asking you if you are
interestedin a penpal, but by the time you read this letter, it is too
late.
The "trojan horse" virus will have already infected the boot sector of
your hard
drive, destroying all of the data present. It is a self-replicating
virus,
and once the message is read, it will AUTOMATICALLY forward itself to
anyone
who's e-mail address is present in YOUR mailbox!
This virus will DESTROY your hard drive, and holds the potential to
DESTROY
the hard drive of anyone whose mail is in your inbox, and who's mail is
in
their inbox, and so on. If this virus remains unchecked, it has the
potential
to do a great deal of DAMAGE to computer networks worldwide!!!!
Please, delete the message entitled "PENPAL GREETINGS!" as soon as you
see it!
And pass this message along to all of your friends and relatives, and
the
other readers of the newsgroups and mailing lists which you are on, so
that
they are not hurt by this dangerous virus!!!!




Join the Crew

Circulating the Internet is an email message entitled "Join the Crew". For a
virus to spread, it must be executed. Reading a mail message does not execute
the mail message. Trojans and viruses have been found as executable
attachments to mail messages, but they must be extracted and executed to do
any harm.

CIAC still affirms that reading E-mail, using typical mail agents, can not
activate malicious code delivered in or with the message.

IMPORTANT - VIRUS Alert!!!


Take note !

Someone got an email, titled as JOIN THE CREW.
It has erased his hard drive.
Do not open up any mail that has this title.
It will erase your whole hard drive.
This is a new email virus and not a lot of people know about it,
just let everyone know, so they won't be a victim.

Please e-mail this to everyone you know!!!
Remember the title : JOIN THE CREW

Variants of this email message are circulating the Internet. If you receive
an email message entitled "Join the Crew" and it has an attachment, CIAC
recommends that you delete the message and the attachment. If you receive
just the message, delete the message. Please DO NOT circulate unvalidated
virus alerts.

**************************************************************************
Mike O'Keefe





From: Barbara Foster :      mme-at-map.com
Date: Thu, 04 Sep 1997 18:16:31 -0700
Subject: Light microscopy course hosted by Providence College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {340F5D6F.6A1E-at-map.com}

Course Announcement: "Optimizing Light Microscopy"
When/Where:
"Optimizing Light Microscopy"
Hosted by Providence College Dept. of Biology (Providence, RI)
October 3, 1997
What: a lively, fast-paced slide lecture and demonstraton for anyone who
uses or plans to use a light microscope: students, teachers, medical
technologists, clinicians, pathologists, and lab managers. Beginning to
more experienced practitioners welcome.

For details...
(a) read below
(b) send for brochure
Program:
1. A quick tour around the microscope - getting to know the bits & pieces
2. Koehler illumination & you: 4 critical steps for aligning you and your
microscope to reduce headaches, fatigue, and errors
3. Care and cleaning
4. Useful principles for understanding and optimizing imaging
5. Putting the basics to work:
a. Troubleshooting
b. Understanding Phase and Hoffman Modulation Contrast
6. The Video connection: cameras, computers, and your microscope
7. Bringing out the best: quick, easy, and often free techniques for
improving contrast
8. Advanced contrast techniques: Fluorescence and DIC
9. Becoming a better consumer: matching your microscope to your
application
10. Questions, Answers, and Information Exchange
(Note: Instructor may vary class content slightly to meet the needs of
participants. Instructor for this course: Barbara Foster of
Microscopy/Microscopy Education)

Free with your tuition: "Optimizing Light Microscopy for Biological and
Clinical Laboratories" (Kendall-Hunt, 1997). Nearly 200 pages of helpful
hints, quick experiments, and new ideas for getting the best from your
microscope. Hot off the presses!

CEU's: 0.6 CEUs, 6 P.A.C.E CEU's
Microscopy/Microscopy Education adheres to the guidelines
established by the IACET.

Pricing:
$150 (includes tuition, breaks, course materials, and copy of book)
*****Save $25 if paid by 9/22/97 *******
Send three from the same facility and save $50 on tuition for the third
person.

Refund policy:
Full refund for cancellations made by 10/17/97. After that date, 50%
refund or full credit for future class. Substitutions accepted.

Questions: call Barbara Foster or Dr. Ken Piel at MME: (413)746-6931

Registration: Download the form below and fax to (413)746-9311 or call
(413)746-6931 and ask for Ken.

Check course you will be attending:
___ Optimizing Light Microscopy (#971003)

Name: _______________________________________________________________
School/Hospital/Company: ____________________________________________
Address: ____________________________________________________________
City/State/Zip: _____________________________________________________
Phone: ______________________________________________________________
Fax: ________________________________________________________________
Email: ______________________________________________________________

Method of Payment:
____ Check enclosed for $ _______________
____ Visa
____ Mastercard
Name on credit card: __________________________________
Credit card number: ___________________________________
Expiration date: ______________________________________
***If billing address is different from one shown above,
please show billing address below:
_______________________________________________________
_______________________________________________________
_______________________________________________________




From: Peter Ingram :      p.ingram-at-cellbio.duke.edu
Date: Thu, 4 Sep 1997 19:06:39 +0100
Subject: North Carolina Meeting Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is a MIME-encapsulated message
If you read this, you may want to switch to a better mailer
--__==========00000000158236==cellbio.duke.edu==__
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 8bit

************** Attention all microscopists who might wish to spend a
weekend on the coast of North Carolina at THE most beautiful time of the
year!!! **********

YOU ARE CORDIALLY INVITED TO PARTICIPATE IN ONE OF THE MORE FUN MEETINGS
YOU ARE LIKELY TO ATTEND DURING THE NORMAL COURSE OF EVENTS!

I have attached a textfile for easy download, and also include the complete
text as part of this message for those of you who prefer it this way!

Hope to see you in October!




THE
NORTH CAROLINA SOCIETY FOR MICROSCOPY
AND MICROBEAM ANALYSIS

presents the

SIXTEENTH ANNUAL
SYMPOSIUM ON
ADVANCES IN MICROSCOPY

"Correlative Microscopy in Biological and Physical Sciences"
Blockade Runner Resort, Wrightsville Beach, North Carolina
October 17-19, 1997

**************** FOR MORE INFORMATION PLEASE CONTACT PETER INGRAM OR ANN
LEFURGEY AT: p.ingram-at-cellbio.duke.edu or a.lefurgey-at-cellbio.duke.edu
*************** or READ ON!

Symposium Description
The Sixteenth Annual Symposium, sponsored by the North Carolina
Society for Microscopy and Microbeam Analysis (NCSMMA), has been planned
with a theme of "Correlative Microscopy in Biological and Physical
Sciences." Continuing with the tradition of the symposium, the guest
lecturers are composed of both nationally and internationally distinguished
scientists. The meeting has several purposes, not the least of which is to
draw attention of the scientific community to emerging developments in the
practical and basic research aspects of exciting new fields, and to bring
people together from diverse disciplines to discuss how innovative
techniques will be relevant to the future direction of microscopy and
microprobe analysis. In particular, this year, special emphasis will be
placed on how correlations between the many forms of microscopy are having
significant impact in the biological and physical sciences.
The symposium also offers an opportunity for interested
participants to submit abstracts of related studies for poster display.
Three special workshops/tutorials (SEE BELOW) will be offered at no
additional charge to participants in the Symposium: (a) Introduction to
Scanning Probe Microscopy, ( b) Confocal Microscopy, and (c)
Immuno-labeling. These are practical, introductory workshops/tutorials and
no previous experience or knowledge is necessary.
The annual business meeting of NCSMMA will he held on Saturday,
October 18th just prior to lunch.

NCSMMA Student Prizes
Student prizes will be awarded at this annual meeting. A total of five
awards will be granted to five different students in the following
categories:
Biological sciences - one abstract (platform presentation) and two
posters;
Physical sciences - one abstract (platform presentation) and one poster

September 29th is the submission deadline for abstracts of platform and
poster presentations. The five winning students will each receive a
$100.00 check as well as complimentary registration fees. All abstracts
will be pre-judged by NCSMMA members, with platform presentations by the
winners, and the posters will be judged at the time of the meeting.
Candidates must be members of NCSMMA and be full-time students to be
eligible for the competition, and must submit an abstract by the deadline
to be considered for either platform or poster awards.

Registration Fees, Hotel rates
The $90 ($100 on site) per person and $50 for students ($60 on
site) registration fee includes: symposium attendance and materials,
Saturday lunch, breaks, and Friday and Saturday evening meals. Additional
Friday evening tickets are available for Adults - $20; Children 10 years of
age and under - $10. Additional Saturday evening tickets are available for
Adults - $20; Children 10 years of age and under - $10. There is a $15
fee for all cancellations. Blockade Runner Resort Hotel special rates
start at $69/night including full breakfast.

For questions or further information, please telephone Betty Gooch,
Duke University Medical Center: (919) 684-3534 or email:
b.gooch-at-cellbio.duke.edu.

PROGRAM

Friday, 17 October, 1997
4:00 - 6:00 pm REGISTRATION and refreshments - Blockade Runner at
Wrightsville Beach
6:30 - 8:30 pm Evening Buffet - pool side at the Blockade Runner,
Wrightsville Beach
Courtesy of JEOL (USA) Inc. and GATAN, Inc.
Beverages courtesy of AMRAY Inc.
(Complimentary with Registration; Adult and Children Guests $20/$10).

Saturday, 18 October 1997

8:30 - 11:30 am Special Workshops/Tutorials on:
(a) Introduction to Scanning Probe
Microscopy (hands on participation)
(b) Confocal Microscopy (hands on
participation)
(c) Immunolabeling with Colloidal Gold
9:00 - 11:00 am Coffee, juice, and cookies - Blockade Runner
10:00 - 11:30 am Poster Session, Exhibitors' Displays - Blockade Runner
11:30 - 12:00 Noon NCSMMA Annual Business Meeting
12:00 noon- l:00 pm Lunch - Casual buffet - Poolside


1:00 - 1:15 pm - Welcome -

1:15 - 1:45 pm Correlative Microscopy in Biological Problem Solving
Ralph Albrecht
2:00 - 2:30 pm Applications of Scanning Probe Microscopy
Chuck Mooney
2:45 - 3:15 pm COFFEE BREAK
3:15 - 3:45 pm Diagnostic Microscopy in the Pharmaceutical Industry
Ruth Lightfoot
4:00 - 4:30 pm Electron Backscatter Diffraction in the SEM
Joe Michael
4:45 - 5:15 pm Project MICRO: Its Realization and Implementation
Caroline Schooley

5:30 - 6:30 pm Poster Session, Exhibitors' Displays
6:30 - 7:00 pm Cocktails, refreshments
7:00 pm Evening Buffet- Al Fresco at the Blockade Runner, Wrightsville Beach
Supported in part by Oxford Instruments, Leo and Zeiss

Sunday, 19 October 1997

8:20 - 9:00 am Student Awards and Presentations
9:00 - 9:30 am Correlative Microscopy in Materials Science
Mike Kersker
9:45 - 10:15 am Cell Growth Studies with Confocal Microscopy
Nina Allen
10:30- 11:00 am COFFEE BREAK
11:00 - 11:30 am EFTEM Techniques in Materials Science
Jim Bentley
11:45 - 12:15 Noon Medical Applications of Correlative Microscopy
David Howell
12:30 pm Finis


Accommodations
Special arrangements have been made to provide a wide variety of
accommodations. Use the reservation form immediately and send it directly
to the hotel of your choice (except Blockade Runner, which must be
telephoned directly) or telephone another hotel directly. Make sure to
mention that you are attending the Duke Microscopy Symposium so that you
will receive the special rates provided for our registrants.


MAKE YOUR RESERVATION NOW!

A one (l) night's deposit is required to hold reservations.
All rates quoted are excluding tax.

Wrightsville Beach, NC (Lumina Avenue)
* *BLOCKADE RUNNER RESORT. 1-800-545-5494. Ask for Code #5067.
Harbor front $69/single; $77 dbl; ocean front, $89 single/$97 dbl.
All rooms include breakfast.
* Waterway Lodge (located at drawbridge). 1-800-677-3771. $55/ two
dbl. beds or queen size. Condo $65/night. Includes queen and sleeper sofa
plus full kitchen..
* Shell Island (at Wrightsville Beach). 1-800-689-6765. Double
occupancy suites only. $129/night.
* Hampton Inn, 1989 Eastwood Road. 1-919-0256-9600. $89/king or two
queen beds plus includes continental breakfast with local calls.
* Meeting site

Wilmington, NC (Market Street)
* Greentree Inn, 1-910-799-6001. $43.95//two dbl. beds w/
continental breakfast.
* Holiday Inn of Wilmington, 1-910-799-1440. $75/king or two double
beds.
* Howard Johnson Plaza, 1-800-833-4721. 89/night, two double beds or
one king size bed.
* Days Inn, 1-910-799-6300. $58.88//two dbl. beds. or 1 queen


************* Send checks payable to: "Sixteenth Annual Symposium on
Advances in Microscopy"
Send to: Betty P. Gooch, Symposium Coordinator
Analytical Electron Microscopy Facility
Box 3709
Duke University Medical Center Tel: (919) 684-3534
Durham, NC 27710 email: bgooch-at-cellbio.duke.edu
Fax: (919) 681-8419


PLUS!!!!!

3 SPECIAL FREE WORKSHOPS!

TO BE HELD IN CONJUNCTION WITH THE

SIXTEENTH ANNUAL SYMPOSIUM
ON
ADVANCES IN MICROSCOPY

Sponsored by the North Carolina Society for Microscopy and Microbeam Analysis
at the
Blockade Runner Beach Resort
Wrightsville Beach, North Carolina
October 17-19, 1997


INTRODUCTION TO SCANNING PROBE MICROSCOPY: Application to Materials Science
& Biology
Chuck Mooney, Park Scientific Instruments, Sunnyvale, Ca.

Basics of STM and AFM

* Contact, non-contact and tapping microscopy * Problems and
solutions
* Lateral force microscopy
* Operating parameters
***** Hands on participation ******



CONFOCAL MICROSCOPY
Nina Allen, North Carolina State University, Raleigh, NC

Basic Operating Principles

* Problems, solutions and limitations
* Laser sources * Confocal versus conventional
fluorescence imaging
* Slit and pinhole apertures and deconvolution
* Fluorescence throughput * 3-D imaging
* Alignment of optics * Judicious use of dyes
* Sensitivity and background subtraction

***** Hands on participation ******



IMMUNOLABELING WITH COLLOIDAL GOLD
Ralph Albrecht, University of Wisconsin, Madison, WI

* Production of gold colloids
* Conjugation of antibody/gold ligand to colloids
* Basics of labelling with colloidal gold conjugates
* Techniques for silver enhancement of gold colloids
* Problems, solutions and limitations

Use of gold conjugates as labels in correlative microscopy


--__==========00000000158236==cellbio.duke.edu==__
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 8bit

Peter Ingram
Sr. Physicist RTI
Adj. Professor of Pathology, Duke University Medical Center
Box 12194
RTP NC 27709

Tel: 919 541-6598 (am) or 919 684-3534 (pm)
Fax: 919 660-2671

--__==========00000000158236==cellbio.duke.edu==__
Content-Type: application/mac-binhex40;
name="16th Symp.txt"; X-Date-Created="Fri, 01 Jan 1904 00:00:00"; X-Date-Modified="Fri, 01 Jan 1904 00:00:00"


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P)'eTBh*[Ff0[F(N0$5E5!!!:
--__==========00000000158236==cellbio.duke.edu==__--




From: Marek Malecki :      malecki-at-MACC.WISC.EDU
Date: Thu, 4 Sep 1997 18:52:31 -0700
Subject: Cryo-ultramicrotome wanted.

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We are interested in buying a used cryo-ultramicrotome.
LKB, Reichert, or RMC acceptable.
Please contact:
Marek Malecki, PI
Phone: 6082638481.
Fax: 6082654076.
Email: malecki-at-macc.wisc.edu






From: Chao-ying Ni; Office 312 SPL; Phone 1013 :      ni-at-me.udel.edu
Date: Thu, 4 Sep 1997 20:41:24 -0400 (EDT)
Subject: PLM sought

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Greetings.
One of my colleagues is seeking a polarized light microscope. A used Nikon
is preferred. Any information is highly appreciated.

Chao-Ying Ni
Batta Laboratories Inc.
6 Garfield Way
Delaware Industrial Park
Newark, DE 19713





From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 5 Sep 1997 12:31:17 +1100
Subject: Re: Discarding Uranyl Acetate

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Could I suggest the original poster try the Safety group. The address is

LISTSERV-at-UVMVM.UVM.EDU
Write SUB SAFETY in the text area.


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 1614
Sydney
AUSTRALIA 2001






From: Jennifer T. Morse :      jmorse-at-snet.net
Date: Thu, 04 Sep 1997 22:51:53 -0400
Subject: optical microscopy question

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I was recently given the following homework assignment: Actual
resolution as determined by measuring the smallest distance between two
distinguishable points in a suitable specimen (resolution standard), is
usually greater than the linear resolution of the microscope. Describe
the factors that contribute to this discrepency. Find and list suitable
good references. Can you give me any help in solving this problem? Do
you know of any good sources to speak to? Do you know the answer? Thank
you for your help, Fred Meisenkothen




From: Chun Hua Kong :      kong-at-materials.unsw.edu.au
Date: Fri, 5 Sep 1997 13:28:35 +1000 (EST)
Subject: EDS for Al-alloys

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Dear microscopists,

I think that there must be somebody who have got experience with
EDS for Al-alloys. I hope you can shine some lights on my silly questions.

We have several students working on Al-alloys (e.g. Al-Fe-Ti,
Al-Fe-Cr et al) using arc melting. The problem is that the EDS results
showed that all the alloys (as-cast or following solution annealing) lost
about 20at% Al (for example, {20% for the nominal composition 25%)?! We
tried on two machines, An-10000 EDS on JEOL 840 (over 10 years old) and
Oxford ISIS EDS on FESEM Hitachi S-4500 (only 1 year old). They showed
similar results at work conditions of 20kV, } 1000cps, for 100s on well
polished samples.

Of course, there are several posibilities for the large amount of
Al loss. Weight ratios have been double checked. Melting under Argon would
cause little loss of Al because the melting point of Al is reletively
lower than others, and some dark dust did appear in the furnace, but this
error should be limited in +/-0.5%. Where did the Al go?

So, I wonder if there is anything wrong with the standardless
analysis of EDS. We have tested the EDS with stanless steel sample. The
results were well consistant with the nominal compositions. Is that
because there are something wrong with the standard data of Al in the
computer, or calibration, or work conditions, or something else? Any idea?
Please help.

Charlie Kong
Kong-at-t-rex.materials.unsw.edu.au






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 4 Sep 1997 22:20:23 -0600 (MDT)
Subject: Re: Discarding Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
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} Could I suggest the original poster try the Safety group. The address is
}
} LISTSERV-at-UVMVM.UVM.EDU
} Write SUB SAFETY in the text area.

Good suggestion. There was a discussion within the last few years on this
topic on the safety listserv. The folks at the University of Vermont who
run the list also have an excellent website at: {http://siri.org} where
they archive messages from the listserv, as well as maintain access to MSDS
information and other safety related information. Full instructions for
subscribing to the list are also there.

John
chandler-at-lamar.ColoState.EDU






From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Fri, 5 Sep 1997 09:18:12 +0200 (MET DST)
Subject: Re: Virus Warning

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From {http://ciac.llnl.gov/ciac/CIACHoaxes.html}

Among mostly serious information it also contains the following spoof of
the Good Times virus hoax that is just too good not to repost here,
although it of course has nothing whatsomuchever to do with translation.
Same could be said about Join the crew, I believe...

READ THIS:

Goodtimes will re-write your hard drive. Not only that, but
it will scramble any disks that are even close to your computer. It
will recalibrate your refrigerator's coolness setting so all your ice
cream goes melty. It will demagnetize the strips on all your credit
cards, screw up the tracking on your television and use subspace
field harmonics to scratch any CD's you try to play.

It will give your ex-girlfriend your new phone number. It
will mix Kool-aid into your fishtank. It will drink all your beer and
leave its socks out on the coffee table when there's company coming
over. It will put a dead kitten in the back pocket of your good suit
pants and hide your car keys when you are late for work.

Goodtimes will make you fall in love with a penguin. It will
give you nightmares about circus midgets. It will pour sugar in your
gas tank and shave off both your eyebrows while dating your
girlfriend behind your back and billing the dinner and hotel room to
your Discover card.

It will seduce your grandmother. It does not matter if she
is dead, such is the power of Goodtimes, it reaches out beyond the
grave to sully those things we hold most dear.

It moves your car randomly around parking lots so you can't
find it. It will kick your dog. It will leave libidinous messages on
your boss's voice mail in your voice! It is insidious and subtle. It
is dangerous and terrifying to behold. It is also a rather
interesting shade of mauve.

Goodtimes will give you Dutch Elm disease. It will leave the
toilet seat up. It will make a batch of Methanphedime in your bathtub
and then leave bacon cooking on the stove while it goes out to chase
gradeschoolers with your new snowblower.

Listen to me. Goodtimes does not exist.

It cannot do anything to you. But I can. I am sending this
message to everyone in the world. Tell your friends, tell your
family. If anyone else sends me another E-mail about this fake
Goodtimes Virus, I will turn hating them into a religion. I will do
things to them that would make a horsehead in your bed look like
Easter Sunday brunch.








From: Toth Attila :      tothal-at-falcon.mufi.hu
Date: Fri, 5 Sep 1997 12:02:54 +0200
Subject: Imaging CdS nanoparticles

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Colleagues,
I am wondering how one would keep the particles from falling through the
open holes since the particles in general are smaller than the size of any
holes in a holey film. Also, how do your "Ultra-thin carbon films" differ
from the ones I
purchase from Agar Scientific in the UK and from SPI in the USA? Are they
really "thinner" on the basis of some measurement and if so, what
measurement do you use?

Sincerely


.attila(L)


Attila L. Toth
------------------------------------------
MTA MFKI
Research Institute for Technical Physics
of the Hungarian Academy of Sciences
H-1325 Budapest POB 76
tel: (36.1) 169-2100 x 226
fax:(36.1) 169-8037
------------------------------------------
email: tothal-at-mufi.hu (EUDORA:=CDrj =E9kesen!)
------------------------------------------




From: Decalchem-at-aol.com
Date: Fri, 5 Sep 1997 06:13:00 -0400 (EDT)
Subject: Unscribe

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Unscribe




From: Woody.N.White-at-mcdermott.com
Date: Fri, 5 Sep 1997 9:24:00 -0500
Subject: Re: EDS for Al-alloys

Contents Retrieved from Microscopy Listserver Archives
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Really need a bit more information, but...
Have you examined the material for homogeniety? BSE imaging
of a polished surface would be a good start. If you have more
than one phase / precipitates, apparent composition will
be a strong function of where you analyze and the size of
the analysis volume relative to the phases/inclusions.
Generally I have found that inhomogenous materials will
compromise analysis accuracy.



Woody White, Electron Microscopist SEM/EDS/WDS

Work: Mcdermott Technology, Inc.
woody.n.white-at-mcdermott.com
http://www.mtiresearch.com/

Home: woody.white-at-worldnet.att.net
http://www.geocities.com/capecanaveral/3722


Dear microscopists,

I think that there must be somebody who have got experience with
EDS for Al-alloys. I hope you can shine some lights on my silly questions.

We have several students working on Al-alloys (e.g. Al-Fe-Ti,
Al-Fe-Cr et al)
{snip}

Charlie Kong
Kong-at-t-rex.materials.unsw.edu.au




From: chris gilpin :      cgilpin-at-fs1.sem.man.ac.uk
Date: Fri, 5 Sep 1997 13:55:20 BST
Subject: email error

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Dear All
I was experimenting with new mail filtering and inadvertenetly sent
mail to a number of addressess that were not supposed to be mailed.
Many apologies if you receive that mail either directly or via the
list



Chris


Chris Gilpin
Biological Sciences Electron Microscope Unit
G452 Stopford Building
Oxford Road
Manchester
M13 9PT
phone +44 161 275 5170
fax +44 161 275 5171
http://www.biomed.man.ac.uk/biology/emunit/emhome.html




From: Lee_ccmail_Wagstaff_at_IRV006-at-ccmailgw.mcgawpark.baxter.com
Date: Fri, 5 Sep 1997 08:25:54 -0500
Subject: Used Denton 502A For Sale

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A friend with a local lab in the Orange County, Ca. area is selling a

fully functional Denton 502A. If interested....contact Dave Ward at

Quikscan Inc. directly. (714) 955-0346.







From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 5 Sep 1997 11:52:12 -0400 (EDT)
Subject: Re: Discarding Uranyl Acetate

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Dear John & others,

} Here is what I propose: take some epoxy resin and coat the inside of a
} polypropylene beaker with the epoxy by building up layers and polymerizing
} the layers.

} Allow the uranium liquids to evaporate
} to dryness (taking care to avoid generating dust) and apply another layer
} of epoxy over the uranium salts.

I have to emphasize the point about avoiding dust. Uranium is an
alpha-emitter (not a beta-emitter as another poster said, although some
of the daughter nuclei emit betas), and the range of these alphas is so
short that they will not penetrate the dead layer of the skin. Thus, uran-
ium is not dangerous unless it is ingested or inhaled. If, however, uran-
ium is inhaled, particles sitting on the lung cells will provide a very
large dose to those cells, and are a serious carcinogen. It is much safer
for everyone if there is NO chance of producing dust or droplets containing
uranium; I'd even be very careful about pouring the liquid into a beaker.
Yours,
Bill Tivol





From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 05 Sep 97 15:55:03 -0500
Subject: Re: Imaging CdS nanoparticles

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Attila L. Toth wrote:
==================================================
I am wondering how one would keep the particles from falling through the
open holes since the particles in general are smaller than the size of any
holes in a holey film. Also, how do your "Ultra-thin carbon films" differ
from the ones I purchase from Agar Scientific in the UK and from SPI in the
USA? Are they really "thinner" on the basis of some measurement and if so,
what measurement do you use?
=================================================
You are right, CdS nano- particles, at least the ones we have seen, those
that would be thin enough to "see" through, are smaller than the smallest
holes we have ever seen anyone able to make in a "lacy" film. So I think
that there might have been some misinformation accidently posted about this
a while back (see Aug. 11 posting DUNNTEM-at-aol.com working for Ted Pella, Inc
.). If I am wrong about this please correct me and set the record straight
.

With regard to so-called "ultra-thin carbon films", obviously carbon films
can be made almost infinitely thin, but they do have to have enough mass to
be self-supporting on the grid mesh being used. That minimum amount of mass
then is going to be a function of the mesh size, the lower the mesh size (e.
g. larger the hole), the more durable (a.k.a. thicker) must be the carbon
film. If thickness of the carbon film is crucial, e.g. you want it
completely minimized, I would recommend our finest, which is our 2000 mesh
grid. This simple reality is often times missed by people worried about
film thickness (which in fact ought to not even be relavent in the case of a
lacey or holely film, because after all, you are getting your information
through the holes, not through the "lacy" areas). So the whole need to
worry about "thickness" per se really ought to be, in the case of lacey
films, a non-issue!

But addressing the subject of carbon film thickness generally, when one does
have the need to "look through" the film, the philosophy of "one thickness
fits all" is not one to which we subscribe. One can have the luxury of
having that philosophy only if there are in-house facilities available to
quality check what has been made, otherwise the customer ends up doing the
quality checking. And finding out at the last minute that coated grids are
not stable and can not be used is usually not a very pleasant discovery.

So while I can not comment on the methods used by our competitors to measure
film thickness, I can tell you how we do the "test" and that is, as we are
making them, and we strive for the minimum possible amount, before we have
made too many, we walk across the hall and put them into a TEM that is
dedicated (after 5:00 pm) for this purpose, that is, to do our own
"clinical" test to make sure that the film is sufficiently durable (e.g.
thick enough) for that given mesh size being used. Now you might not
believe this, but the instrument used for the testing is an RCA EMU 4-B,
manufacturerd by RCA in 1969. So we think of this as a worst case test. If
the carbon film is stable enough to survive the beam of an RCA EMU-4B, an
instrument featuring technology more than thirty years old, surely it ought
to be more than acceptable in an instrument of more modern manufacture
(assuming minimum column cleanliness). And that kind of test procedure
results in a history of almost (note I said "almost") no returns!

Interestingly enough, the films do not have to be "thicker" to be stable in
the RCA than in our much newer JEOL TEM. A film with minimum thickness,
stable in one, seems to be similarly stable in the other.

More information about our custom coated carbon grids can be found on our
website given below.

Disclaimer: SPI has produced custom coated grids for customers for more
than twenty years so we have an interest in promoting our carbon coated
grids. The posting I characterized as containing "misinformation" came from
a competitor, so everything I said should be taken within that context.

Chuck


===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 05 Sep 97 17:07:27 -0500
Subject: HAZMAT shipping regulations

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:
=================================================
But too many regulations are not wise - eg. the dangerous goods shipping
laws which appear designed to make things more expensive but not safer.
=================================================
We should all be taking the HAZMAT reguations, no matter where we live,
seriously. I can not comment about the laws in Australia, but I have a very
high regard for the regulations promulgated by the U. S. Dept. of
Transportation (DOT) and IATA (for international shipments) as well as the
reasons behind them. And I can state, from first hand experience, that if
the regulators are presented with sound technical information for a
reguation to be changed, they will indeed listen (at least in the US) and
sometimes make changes.

If anyone remains unconvinced, about the importance of adherence to all
HAZMAT regulations, keep in mind that the ValuJet disaster was caused by
someone not taking seriously these same regulations.

While it is correct that one can incur almost unbelievalbly high shipping
costs for HAZMATs, this is not always the case. In many instances, such as
for the ordering of osmium tetroxide, if one orders "smart", they can do
their shipping at costs only nominally more than if the same weight of
tweezers, grids, or SEM mounts were being shipped. Now this would not apply
for everything (e.g. our SPI Dusters, for example) but it does apply for a
surprising number of HAZMATs routinely used in EM labs. We are also in
the process of reformulating some of our embedding kits so they too can be
shipped at the lower prices.

We have tried to explain how a customer can "order smart" on our website,
click on "Hazardous Items(Good News and Bad News)". While savings in
shipping costs for domestic US customers are possible, the real
beneficiaries are foreign customers now no longer are restricted to the use
of air freight (which has associated with it high minimum charges).

And while we are on this subject, just remember, don't ever try to take
HAZMATs in checked airline luggage to save some money, it is unconscienable
from a moral standpoint and puts at risk everyone on the plane flight.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 05 Sep 97 17:07:27 -0500
Subject: HAZMAT shipping regulations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:
=================================================
But too many regulations are not wise - eg. the dangerous goods shipping
laws which appear designed to make things more expensive but not safer.
=================================================
We should all be taking the HAZMAT reguations, no matter where we live,
seriously. I can not comment about the laws in Australia, but I have a very
high regard for the regulations promulgated by the U. S. Dept. of
Transportation (DOT) and IATA (for international shipments) as well as the
reasons behind them. And I can state, from first hand experience, that if
the regulators are presented with sound technical information for a
reguation to be changed, they will indeed listen (at least in the US) and
sometimes make changes.

If anyone remains unconvinced, about the importance of adherence to all
HAZMAT regulations, keep in mind that the ValuJet disaster was caused by
someone not taking seriously these same regulations.

While it is correct that one can incur almost unbelievalbly high shipping
costs for HAZMATs, this is not always the case. In many instances, such as
for the ordering of osmium tetroxide, if one orders "smart", they can do
their shipping at costs only nominally more than if the same weight of
tweezers, grids, or SEM mounts were being shipped. Now this would not apply
for everything (e.g. our SPI Dusters, for example) but it does apply for a
surprising number of HAZMATs routinely used in EM labs. We are also in
the process of reformulating some of our embedding kits so they too can be
shipped at the lower prices.

We have tried to explain how a customer can "order smart" on our website,
click on "Hazardous Items(Good News and Bad News)". While savings in
shipping costs for domestic US customers are possible, the real
beneficiaries are foreign customers now no longer are restricted to the use
of air freight (which has associated with it high minimum charges).

And while we are on this subject, just remember, don't ever try to take
HAZMATs in checked airline luggage to save some money, it is unconscienable
from a moral standpoint and puts at risk everyone on the plane flight.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From: Robert A. CARLTON 610-454-3949 :      CARLTRA-at-rpr.rpna.com
Date: Fri, 05 Sep 1997 17:29:00 -0400 (EDT)
Subject: Re: Optical Microscopy Question

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Jennifer,

With regard to references the following are quite good for sizing in
the optical microscope:

Handbook of Chemical Microscopy by Chamot and Mason

The Particle Atlas, Volume 1 by McCrone and Delly

Particle Size Measurement Vol 1 by Terence Allen

I have some trouble understanding the question, it doesn't seem to
address any issue precisely since it does not specify the optics, the
resolution standard, the wavelength of light used or the method of
measurement. All those factors affect the resolution. Real
measurements are also affected by the contrast in the specimen, the
calibration standard, and such mundane issues as how well the optics
are aligned and adjusted (ie the use of Kohler illumination, etc.) and
how young and fit the eyeballs are doing the measurements - even the
lighting in the room can affect measurements.

The questioner may be after how diffraction affects particle
measurements. The limit of detection of the optical microscope is
much less than the resolution limit. I can detect objects much
smaller than 0.4 um (the resolution limit of my neofluor 40 times
objective with white light) - probably down to 0.1 um, but diffraction
effects make them appear larger so that they all look to be about 0.4
um. Allen's book has a nice description of this effect and the other
two books have good discussions of the origins of the resolution
equation.

Robert Carlton
Rhone-Poulenc Rorer





From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Fri, 5 Sep 1997 16:41:21 -0500
Subject: Dielectric Constant and calibration of intracellular pH

Contents Retrieved from Microscopy Listserver Archives
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Hi- I am forwarding this message for a colleague who asked me this
question. I don't have a clue. Any help appreciated. Dave


} The calibration of the cell for pH measurements using fluorescent probes
} is done by using ionophores such as nigericin or by ratiometric calibration.
} Does any of this method account for the effect of the dielectric constant
} of t
} the medium on the pKa' of the probe ? If not is the effect of the
} dielectric co
} nstant of the medium accounted by any other method ? Is it justified to
} ignore the effect of the dielectric constant of the medium ?
}
} Thank you in advance for your help and would be eagerly waiting for the
} respo
} onses.
} Abizer Harianawala
}

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu






From: RE73-at-aol.com
Date: Fri, 5 Sep 1997 18:09:10 -0400 (EDT)
Subject: Morphometry

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I am having trouble finding information concerning the theroy of morphometry
and its benifits to the biological field... any sugjestions.




From: Neelima Shah :      shahn-at-mail.med.upenn.edu
Date: Fri, 05 Sep 1997 20:49:48 -0400
Subject: Job Posting..

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Dr. Jarett,
The following ad has been posted on the Microscopy listserver which
exclusively serves the em community. I did not include the email address on
purpose to prevent unnecessary postings in your mail box. Hope you have a
pleasant weekend.
Neelima


ELectron Microscopist:

The University of Pennsylvannia School of Medicine Institutional Electron
Microscopy Core is searching for a Co-Director. This Core has the broad
support of the Diabetes Center, the Cancer Center, the Department of
Pathology and Laboratory Medicine, and School of Medicine. The Core is
widely used by medical community, as well as the University as a whole. The
candidate should have a Ph.D. in cell and molecular biology and at least 5
years experience with all aspects of Electron Microscopy. The individual
should also possess administrative experience and computer skills. The
position title will be Senior Research Investigator and will be responsible
for helping the Director develop reports for centers, grants, papers etc.
Salary will be consistent with experience. Respondents should send their
curriculum vitae, bibliography, and 3 references to:
Dr. Leonard Jarett, M.D.
Chair, Department Of Pathology and Laboratory Medicine
Universtiy Of Pennsylvania
6 Gates Building
3400 Spruce Street,
Philadelphia, Pa 19104-4283
Regards...
: ) ; )
Visit us at http://www.med.upenn.edu/~path/core/EMCMAIN1.HTM





From: halldorg-at-iti.is
Date: Fri, 5 Sep 1997 12:58:37 +0000
Subject: Re: EDS of Al alloys

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,

You should always be careful regarding ZAF corrections for mixtures of
light and heavy elements. My advice is, if you don´t have a suitable
standard to test your ZAF corrections, then don´t trust them. You have not
calibrated your ZAF corrections until you test them on a suitable standard.
For mixtures of Al-Fe-Cr, use a similar standard, not just Fe-Cr or Fe
standards.

Halldor Gudmundsson






From: Jim Darley :      jim-at-proscitech.com.au
Date: Sat, 6 Sep 1997 18:07:50 +1000
Subject: Re: Discarding Uranyl Acetate

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Sorry Bill but Uranium emits a whole zoo of radiations, alpha, beta, gamma.
A jar of UA placed on a sheet of fast film for a day or two will expose the
film through the jar.
UA dissolves beautifully in water and the sitting in the lung argument
applies more to other forms of U - like in mining.
I maintain that poring small quantities (I used to discard 5ml/months of a
2% solution) into the sewage system is the most sensible solution. Just sit
down and work out the dilution factor on an annual basis and I expect in
most cities the result will be approximately that concentration of U in
seawater.
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au


----------
} From: William Tivol {tivol-at-wadsworth.org}

}
} Dear John & others,
}
} } Here is what I propose: take some epoxy resin and coat the inside of a
} } polypropylene beaker with the epoxy by building up layers and
polymerizing
} } the layers.
}
} } Allow the uranium liquids to evaporate
} } to dryness (taking care to avoid generating dust) and apply another
layer
} } of epoxy over the uranium salts.
}
} I have to emphasize the point about avoiding dust. Uranium is an
} alpha-emitter (not a beta-emitter as another poster said, although some
} of the daughter nuclei emit betas), and the range of these alphas is so
} short that they will not penetrate the dead layer of the skin. Thus,
uran-
} ium is not dangerous unless it is ingested or inhaled. If, however,
uran-
} ium is inhaled, particles sitting on the lung cells will provide a very
} large dose to those cells, and are a serious carcinogen. It is much
safer
} for everyone if there is NO chance of producing dust or droplets
containing
} uranium; I'd even be very careful about pouring the liquid into a beaker.
} Yours,
} Bill Tivol
}




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sun, 7 Sep 1997 12:19:34 GMT+1200
Subject: Re: EDS for Al-alloys

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Charlie

I would guess that you are trying to push standardless EDS a bit
further than it's intended to go.
Why don't you use some standards?

Ritchie


} We have several students working on Al-alloys (e.g. Al-Fe-Ti,
} Al-Fe-Cr et al) using arc melting. The problem is that the EDS results
} showed that all the alloys (as-cast or following solution annealing) lost
} about 20at% Al (for example, {20% for the nominal composition
25%)?!
} Where did the Al go?
} So, I wonder if there is anything wrong with the standardless
} analysis of EDS.
} Charlie Kong

Ritchie Sims phone: 64 9 3737599 ext 7713
Department of Geology fax: 64 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Childs, Gwen V. :      gvchilds-at-utmb.edu
Date: Sun, 07 Sep 1997 09:28:00 -0500
Subject: Histochemistry Society web page

Contents Retrieved from Microscopy Listserver Archives
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Greetings,

We are continuing to improve the US Histochemistry Society Web page and
welcome your suggestions as to how we can be of better service to the
community.

Right now, we are running a link to the Immunocytochemistry Discussion
Newsgroup. Check
out our page for instructions on how to get involved.
It is: http://www.hcs.microscopy.com

We are also running a contest for the best Logo for our society. We
have ten entries and welcome more. The prize is $200.00. Check out the
Logo contest on the above web site and vote for the
best and submit your own.

Thanks for your attention!

Gwen Childs

*************
Gwen V. Childs, Ph.D.
Professor and Vice-Chair
Department of Anatomy and Neurosciences
University of Texas Medical Branch
Galveston TX 77551-1043
gvchilds-at-utmb.edu
http://cellbio.utmb.edu/childs/childs.htm
(409) 772-1942; FAX 772-3222
Toll Free Pager: 1 888 715-8636






From: Judy Z. Wu :      JWU-at-KUPHSX.PHSX.UKANS.EDU
Date: Sun, 7 Sep 1997 11:14 CST
Subject: help needed to fix a borken EDAX 9100 detector

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I have an old EDAX 9100 detector with broken Be window and photo diode plus
FET. I like to know whether (1) someone could fix it quickly with affordable
price or (2) some information on where we could buy the Be window and the
diode-FET unit, plus tips to fix the detector. My students and I would
appreciate any help from you and we hope we could have our detector back
to work so we could continue our experiments.

Thanks so much,

Judy Wu
Dept. of Physics
Univ. of Kansas
Lawrence, KS 66045
(785)864-3240 (phone)
(785)864-5262(fax)




From: adtservices-at-1stfamily.com
Date: Sun, 7 Sep 1997 13:17:43 -0500
Subject: Attn All Businesses : Increase Prof

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///////////////////////////////////////////////////////////////////////////////
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you wish to be removed from this advertiser's future mailings, please reply
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From: Patrick Huddie :      phuddie-at-microcosm.com
Date: Sun, 07 Sep 1997 18:00:09 -0400
Subject: Microscopy Position Available Now

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This is a multi-part message in MIME format.
--------------D0A6E5E30BCDAEC778B68638
Content-Type: text/plain; charset=iso-8859-1
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: quoted-printable
X-MIME-Autoconverted: from 8bit to quoted-printable by iris.microcosm.com id SAA03611

Dear Microscopists:

We would like your help in locating the best candidate for the position
of Director for our Applications Laboratory. The following text and
attachment describe the opportunity. Would you please post the
advertisement and bring it to the attention colleagues?

IMAGING RESEARCH OPPORTUNITY

We are seeking a talented individual to direct our Applications
Laboratory, leading research into fluorescence detection, confocal
infrared and ultraviolet microscopy, and x-ray microscopy. The
incumbent will also participate in the specification and design of novel
optical systems developed by the Engineering Department. The
Applications Laboratory patents and publishes research findings,
supports Microcosm=92s customers=92 in their use of our technology, and
communicates the implications of internal and external research to the
Marketing and Engineering Departments.

The position requires a Ph.D. in cell biology or a related science, with
primary experience in microscopy and imaging, and a strong physical
science background. Familiarity with the latest microscopy techniques
is mandatory. It is essential that the incumbent has sound knowledge of
optics and digital image analysis, both in theory and in practice.
Ideally candidates will have experience with several imaging platforms,
including isee and dsp/os, NIH-Image, Image Tool, Metamorph & Image-1,
as well as the LSM and MPM instruments made by Carl Zeiss.

The compensation package for the right candidate is negotiable.
Benefits will include medical and dental insurance, and participation in
our profit-sharing 401(k) retirement plan.

Interested persons should send their r=E9sum=E9 to the attention of Dr.
Patrick Huddie at the address above. Please direct e-mail to
phuddie-at-microcosm.com.

--------------------------------------------------------------
Dr. Patrick L. Huddie (301) 725-2775 Fax (301) 725-2941
Microcosm, Inc., 9140 Guilford Road, Suite O, Columbia, MD 21046
e-mail phuddie-at-microcosm.com URL http://www.microcosm.com
The Web is:"Vaster than empires and more slow" Andrew Marvell
(1621-1678)


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From: Melvyn Dickson :      M.Dickson-at-unsw.edu.au
Date: Mon, 08 Sep 1997 08:48:52 +1000
Subject: Reference on Biological Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A good place to start is "The Image Processing Handbook" Second Edition.
John C. Russ. CRC Press.
ISBN 0-8493-2516-1


Mel Dickson
President, Australian Society for Electron Microscopy
Director, Electron Microscope Unit,
University of New South Wales.
Sydney NSW 2052 Australia

Phone (+612) 9385-6383
Fax (+612) 9385-6400

Website {http://emunit1.babs.unsw.edu.au/emu_top.htm}




From: maria lucia ribeiro caldas :      caldasml-at-amcham.com.br
Date: Sun, 07 Sep 1997 20:16:13 -0300
Subject: TEM- skin biopsies pproblems

Contents Retrieved from Microscopy Listserver Archives
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I am having technical problems in EM skin biopsies. I would like to have
a detailed protocol for processing skin biopsies.




From: SEMTRADER-at-aol.com
Date: Sun, 7 Sep 1997 19:13:35 -0400 (EDT)
Subject: Re: help needed to fix a borken EDAX 9100 detector

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In a message dated 97-09-07 15:20:18 EDT, JWU-at-KUPHSX.PHSX.UKANS.EDU writes:

} Subj: help needed to fix a borken EDAX 9100 detector
} Date: 97-09-07 15:20:18 EDT
} From: JWU-at-KUPHSX.PHSX.UKANS.EDU (Judy Z. Wu)
} To: microscopy-at-sparc5.microscopy.com
}


Have you tried the fellow at Evex

Call them at 609.252.9192
or on the Web www.evex.com




From: Chun Hua Kong :      kong-at-materials.unsw.edu.au
Date: Mon, 8 Sep 1997 12:01:59 +1000 (EST)
Subject: More ? on EDS for Al-alloys

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Hi,
Thank you who kindly responded to my question on EDS for
Al-alloys. Troublesome students always raise extraordinary questions to
test the range of knowledge (and/or self-confidence) of their teachers.
Sometimes, they even try to meltdown the reputation of modern technique
with the spark of their intuition. Now, let's show our royalty to the
advanced materials science.
The big question mark has not been erased yet, although the
simplest answer, which may be the right one and has been selected, is to
blame the students who prepared the dummy samples. Who knows whether they
swallowed a piece of aluminium down before melting or not?
There were some beginners who asked me quite often about the error
range and sensitivity of EDS. My answer is that at first it depends upon
your sample (Am I a good lawyer?); secondly, in micro-scale few thing is
uniform (Do not blame me if the results are unrepeatable); at last, I
would say that in ideal testing conditions the error should be less than
+/-0.5% in general(I hope so indeed!). They were quite happy to use the
machine.
Several experts have advised me that we should give up the
dependence upon the standardless analysis of EDS, although the others
showed strong evidence to prove that it is working well. Faster would
never be safer, just like driving a car. This should not imply that the
results of "semi-quantitative analysis" can only be trusted in HALF,
neither the error may be 50%. How do you feel? Do you mention "semi-"
quite often to the users?
My former colleague, Bruce, suggested that oily detector window
might cause the problem for softer radiation such as Al-Ka. This message
struck a spark in my poor memory. I really saw somebody who dared to wipe
oil off the thin Be-window of a TN-5400 EDS using a cotton ball with a
drop of acetone. That was ten years ago.
Let's go to the question: Have you ever cleaned the Be window of
your EDS system? If the answer is positive, how often? Or, just wake me up
---- NEVER EVER THINK ABOUT THAT, YOU THE TROUBLE MAKER!
I swear I have never been a trouble-maker. I just want to learn
something from you. Just talking, no action following.
It is too lang. Thank you for your time.

Charlie Kong
kong-at-t-rex.materials.unsw.edu.au






From: Arne Olsen :      arne.olsen-at-fys.uio.no
Date: Mon, 08 Sep 1997 11:06:13 +0200
Subject: Professorship in Physics (Materials science/Structure physics)

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A professorship due to the retirement of Professor Jon Gj=F8nnes at
University of Oslo has been announced. The professor is expected to
strengthen the reseach activity of the department in materials
science/structure physics. The activity is mainly concerned with the
application of electron-optical techniques to metallurgical questions,
ceramic and semiconductors and with the development of quantitative methods
for obtaining structural information (crystal structure, electronic
structure and disordered structure).

Here follows the official announcement, including the
application procedure. The announcement can also be found in the Journal
Nature. The application deadline is October 3 1997. For additional
information please
contact

Professor Arne Olsen
Department of Physics/Centre for Materials Science
Gaustadalleen 21
0371 Oslo
Norway
Tel. (+47) 22 95 87 40
Fax: (+47) 22 95 87 49
e-mail: arne.olsen-at-fys.uio.no



Professor in Physics (Materials science/Structure physics)

The Faculty of Mathematics and Natural Sciences at the University of Oslo
invites applications for the position of Professor in the Department of
Physics with research interests within the field of materials
science/structure physics.

The Department of Physics at the University of Oslo has 83.5 academic staff
of which 26 are temporary (17 research associates and 9 adjunct professor
positions). Further,there are about 45 funded by external sources. The
department has 38 technical staff positions and 9 administrative staff
positions.

Teaching in the Department is directed towards the degrees cand. mag.
(approx. B.Sc.), siv.ing. (M.Eng), cand.scient. (M.Sc.) and dr.scient.
(Ph.D). There are currently 110 students enrolled in Masters programmes and
70 in doctoral programmes.

Research in the department is organised in 8 groups which undertake both
experimental and theoretical studies: Biophysics, Electronics, Elementary
Particle Physics, Condensed Matter Physics, Nuclear and Energy Physics,
Plasma and Space Physics and Structure Physics. In addition there is a
Theoretical Physics Group.=20

The vacant professorship is connected to the Structure Physics Group, which
also is part of the Faculty's Centre for Materials Science. The Structure
Physics Group, localized in the Oslo University Research Park, runs an
electron microscopy and metallographic laboratory with two transmission
electron microscopes (JEOL 200CX and 2000FX) equipped for analysis (EDS,
EELS, TV-system), optical microscopes and image analysis. A new TEM
instrument will be installed in 1998. The research group, which numbers
20-25 students and staff, has extensive collaboration with other research
groups within the University and in Norwegian industrial and public
research laboratories. The group also maintains strong international
contacts. The academic staff are engaged in the University's teaching
programme for materials science as well as in general physics teaching in
the department.=20

Much of the research in the group is linked to the application of
electron-optical techniques to metallurgical questions, ceramics and
semiconductors and to the development of quantitative methods for obtaining
structural information (crystal structure, microstructure, electronic
structure and disordered structure).

The professor will be expected to strengthen the research activity in
Structure Physics as well as the operation of the group's laboratories. The
successful applicant must be able to document scientific expertise in one
or more of the group's major activities.=20

The appointee must be able to provide supervision at all levels of
teaching. The professor will have responsibility for supervision of masters
and doctoral candidates within her/his special field. The language of
instruction for undergraduate courses is Norwegian, but English will be
accepted for the first three years of appointment. The appointee may also
be required to undertake administrative duties as prescribed in the
applicable University regulations.

According to current regulations, the evaluation of applicants takes regard
of scientific, professional and educational qualifications as well as other
activities, which may qualify the applicant. Where several applicants are
deemed to have equivalent qualifications after evaluation of the
scientific, professional and educational qualifications, a female applicant
will be ranked above a male applicant according to the procedure for the
appointment of professorial staff.

The application shall specify the candidate's education, previous
positions, scientific, professional and educational activities and
administrative experience. Curriculum vitae and publications list are
therefore to be included.

The application shall furthermore include a short description of the
scientific works that the applicant regards as the most significant and on
which the evaluation might especially be based. Normally this ought not
exceed 10 items.

The application is to be addressed to the Academic Collegium, University of
Oslo, and is to be sent with documentation to: Faculty of Mathematics and
Natural Sciences, P.b. 1032 Blindern, N-0315 Oslo, Norway by the
application date. Within one month of this date, the applicant must have
sent to the Faculty's Secretariat:

- 5 complete sets of scientific works, published or unpublished, which one
wishes to be considered in the evaluation (normally not exceeding 10)

- 5 copies of the application with documentation (C.V., complete
publications list, description of the 10 most important works)

Scientific works which are in preparation on the application date may
nonetheless be submitted within three months of the deadline provided the
Secretariat is informed when the remaining works are submitted.

After the application date, the University will send applicants
instructions for submission of scientific works.

One is otherwise referred to the regulations for appointment of
professorial staff approved by the Academic Collegium in accordance with
the University Act =A732.







From: Jim Darley :      jim-at-proscitech.com.au
Date: Mon, 8 Sep 1997 18:13:27 +1000
Subject: Fw: detection limits x-ray

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I thank Garratt-Reed for corroberating my previous posting. John Bazzola
had asked for a rough guide (he has confirmed that since) on detection
limits of EDS versus WDS techniques. Anybody who has any significant
experience with microprobe analysis knows that there is no single line
correct answer. However, it is imperative for analysts to remember a few
general figures so they can advise on appropriate instrumentation and
techniques.

John B's initial inquiry deserved a reply and when none was given, I
posted
mine more than a day later. I believe that my posting is a useful guide
for
non-specialist analysts. Nothing that GR writes makes nonsence of my
posting.

Nobody had asked about other differences between the techniques eg.
resolution or simultaneous acquisition. I am pleased that G-R has supplied
some information on those topics and on new, very high count-rate
acquisition facilities for EDS. G-R is proud of his 0.6% "quantitative
analysis" of Cr at an accuracy of +/- 0.1wt%.

I suggested that in EDS the presence of 1% of an element is the
approxiamte
lower limit for quantitative
analysis. G-R has lowered that limit by some 40% - or has he? +/- 0.1% is
good when 20% of the element is present, as it represents an accuracy of
0.5%. +/- 0.1% when 0.6% is present is about +/- 16% accuracy; I call
that
qualitative or at best semi-quantitative.

Another correspondent emailed me and noted that it was President Trueman
who had been looking for a one handed adviser. But that was for an
economist and
not a scientist as I, apparently wrongly, remembered. The correspondent
could see
my point though; thanks to Brian Demczyk. I think that Trueman had an
excellent idea but he should have extended that search to a scientist as
well. Certainly microscopists and economists share disciplines which
combine
art and science. And I should add require 'good judgement'.

Why now was I abused with that opening: "Jim Darley's posting is hardly
furthering good science". Am I to believe that good science is advanced
by quarelsome nitpicking and that all broadbanding is verboten? I plead
not guilty.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au

} } Subject: Re: detection limits x-ray
} } Date: Friday, 5 September 1997 6:21
} }
} } Jim Darley's posting is hardly furthering good science.
} }
} } 1: Detection limits (John's original question). Modern EDX systems
are
} } easily capable of quantitative analysis in the sub-1wt% range. See,
for
} } example http://prism.mit.edu/facltis/stem/stmexam.htm (the second
} } illustration on that page) where I was getting analyses for Cr in steel
} of
} } the order of 0.6+/-0.1 wt%. I don't know about you, but I consider
this
} } quantitative. This, mind you, was in a measurement where I was
} attempting
} } to optimize spatial resolution rather than sensitivity, and the
} acquisition
} } time was 60sec. per data point. This example, of course, was from a
} STEM.
} } There are many more examples in the literature.
} }
} } Using beam gating techniques such as those developed by Charlie Lyman
and
} } colleagues, EDX data can readily be acquired at 20,000-40,000 counts
per
} } real second, if spatial resolution is sacrificed. Combine this with an
} } acquisition time of 1,000 seconds (by no means unrealistic for an
} important
} } measurement) and the detection limit is in the rage of, or better than,
} 0.01wt%.
} }
} } Of course, in the SEM, which may have been the point of John's original
} } question, the situation is not the same, the beam voltage is lower
} } (resulting in poorer peak/bremmstrahlung ratios) and the emission of
} x-rays
} } from a solid sample is different from that in a thin foil, but these
are
} } some of the variables that Michael quite rightly pointed out must be
} considered.
} }
} } 2: Comparison between EDX and WDX.
} }
} } This is like comparing apples and oranges, because the instruments
} designed
} } with them are generally intended for different purposes.
} }
} } WDX has a much better peak resolution than WDX, which results in better
} } measured P/B ratios (and hence improved statistics), as well as much
} better
} } capability in resolving nearby x-ray lines. Also, because the x-ray
} } counting and wavelength analysis are different functions in the crystal
} } spectrometer, available countrates have traditionally been higher in
WDX
} } than EDX (although modern EDX detectors are an order of magnitude
faster
} } than they were fifteen years ago). Against this must be set the fact
} that
} } WDX is inherently a serial technique (although multiple spectrometers
} help
} } here), while EDX is a parallel technique (compare the advantages of
PEELS
} } over SEELS - although this is not a totally fair comparison). Anyway,
} the
} } advantage of WDX (ignoring the capability of resolving peak overlaps)
is
} } improved statistical precision. Is this useful?
} }
} } Well, maybe.
} }
} } By far the most significant parameter which affects electron-induced
} x-ray
} } emission spectra is sample geometry. Two extreme cases are where the
} sample
} } is a thin foil (as in the STEM) where, to a first approximation, the
} x-ray
} } spectrum recorded by the detector is the same as that emitted, and to a
} } second order, it is possible to derive a reasonable thickness
correction
} for
} } cases where the error is small. Alternatively, when the sample has
} } dimensions large compared with the volume irradiated by the electron
} beam,
} } and has an accurately known geometry compared to the incident beam and
} the
} } detector (such as a polished flat sample in the microprobe), correction
} } programs such as ZAF can do a reasonable job of extracting a
quantitative
} } analysis.
} }
} } What about where the sample geometry is unknown (for example, a rough
} } surface such as might be examined in the SEM)? In that case the
} uncertainty
} } in the analysis is far, far worse than any uncertainty caused by the
poor
} } statistics of the EDX spectrum, so there is no point or advantage
} whatever
} } in using WDX to try to improve things, because it won't. This is why
} } typically an SEM has an EDX detector - it is cheaper and gives just as
} good
} } an analysis (except for the somewhat poorer detection limit). In the
} } microprobe, great care is taken in polishing and mounting the sample,
so
} the
} } advantage of the WDX detector can be realised.
} }
} } Where does this leave us? The advantage of a WDX detector over an
EDX
} } detector *ON THE SAME SAMPLE* is limited - perhaps an order of
magnitude
} in
} } detection limit, and, on a flat, polished sample, also perhaps
} approaching
} } an order of magnitude in precision. The WDX detector can also resolve
} many
} } cases where peaks would overlap in EDX. On general rough SEM samples,
} the
} } only advantages of WDX are a small improvement in detection limits and
} the
} } ability to resolve overlaps (which could be important if a trace
element
} } peak overlaps a major peak in the EXD spectrum.
} }
} } The President's science advisors were right - it all depends. I seem
to
} } remember that one of Jim Darley's Prime Ministers (Gough Whitlam?) had
to
} be
} } dismissed because Parliament would not pass his budget - but does that
} have
} } anything to do with Science?
} }
} } Tony Garratt-Reed
} }
} }
} } } Hi John and Michael et al:
} } } I recall that one of your Presidents (Kennedy?) was looking for a one
} } } handed science adviser; the ones he had always prevaricated "on the
one
} } } hand and on the other hand".
} } }
} } } Michael is quite right, detection limits vary greatly depending on
} endless
} } } factors. However, it is useful to have some figures as guidelines:
} } } Considering say the 10 elements following sodium. Detection of these
is
} } } about best.
} } } For these in EDX the limit for quantitative analysis is about 1%.
} Detection
} } } limit is about 0.1%. Increasing counts and counting times beyond the
} } } customary 100 seconds at perhaps 2000cps will scarcely improve either
} } } limit.
} } }
} } } WDX is near quantitative to its detection limit and that is at least
two
} } } orders of magnitude greater than is EDX.
} } }
} } } I'll enter correspondence only when it concerns errors in excess of
five
} } } orders of magnitude.
} } } Cheers
} } } Jim Darley
} } }
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Phone +61 77 740 370 Fax: +61 77 892 313
} } } Great microscopy catalogue, 400+ Links, MSDS
} } } ************************ http://www.proscitech.com.au
} } }
} } } } John J. Bozzola wrote:
} } } } }
} } } } } Under ideal (and reasonably attainable conditions), what is the
} } } } } detection
} } } } } limit (in grams) for EDX and WDX? Thanks.
} } } }
} } } } The question you ask is not specific enough ... to many factors to
} be
} } } } considered with regard to which elements you are interested in, and
} } } } (e.g.) ... how sensitive your specimen is to long count times and
high
} } } } beam currents ... ... ask again ...
} } } } cheerios, shAf
} } } } --
} } }
} }
} }
} } Anthony J. Garratt-Reed
} } MIT Room 13-1027
} } 77 Massachusetts Avenue
} } Cambridge, MA 02139-4307
} } United States of America
} }
} } Ph: 617-253-4622
} } Fax: 617-258-6478
} }




From: Jim Darley :      jim-at-proscitech.com.au
Date: Mon, 8 Sep 1997 17:31:29 +1000
Subject: Re: shipping regulations

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I gave shipping regulations as an example, where regulations frequently do
not make much sense. While shipping regulations are not directly a
'microscopy matter' they do affect us all.
Clearly, I did not advocate flaunting any regulations; it's foolhardy for
an individual and suicidal for a business. But we are free to discuss: Are
those rules wise, cost efficient and appropriate. It appears Chuck Garber
thinks they are, I beg to differ.
I am only concerned with international airfreight and the IATA defined
'dangerous goods' regulations.
To make shipping safer, regulations can extend to packaging requirements,
maximum quantities shippable, cargo only aircraft requirements for some
goods and 'antidote packing'.
Generally, packing requirements are reasonable.
Weight limits set to exempt 'dangerous goods' are few and generally they
are too high. This means tiny quantities of not particularly dangerous
goods require very expensive shipping.
Antidote packing is basically not used. It makes sense to pack OsO4 in a
tin with a little full cream powdered milk. Vapour from a cracked vial
would be absorbed and it gives the lab a handy supply of that powder to
keep for any laboratory OsO4 problems. We have shipped OsO4 in that manner
for many years now. Packing in vermiculate is less effective than newspaper
- which would at least absorb some of the vapour.
The funniest thing is the paperwork required and this is largely used to
justify the expense of DG shipments. Spot checks and appropriate fines for
non-compliance would be a better preventative than lots of paper work. To
wit: The ValuJet disaster did happen despite those rules.
Dangerous goods, certainly on international routes, go mostly by passenger
aircraft. Picture the site of a crash - who would scramble through all that
useless paper and to what good? The worst is the expense: We work on
airfreight from the US at US$10/kg for the gross weight of medium sized
shipments. For DG that figure for generally larger shipments is US$25/kg -
which frequently is more than the value of the goods. This cost (+paperwork
and extra packing) would be the cost the end-user has to bear if it meant
substantially improved safety. I can see none. The overall global cost of
DG shipments above the cost of normal shipments has to amount to several
billion $ annually. It suits IATA and the airlines and apparently Chuck
Garber.
I submit that we are getting negligible additional safety for thousands of
megabucks. Retaining and improving on the rules for packing and marking of
boxes but severely limiting the paperwork would be a rather more effective
option.
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au

----------
} From: Garber, Charles A. {cgarber-at-2spi.com}
} Date: Saturday, 6 September 1997 8:07
} Jim Darley wrote:
} =================================================
} But too many regulations are not wise - eg. the dangerous goods shipping
} laws which appear designed to make things more expensive but not safer.
} =================================================
} We should all be taking the HAZMAT reguations, no matter where we live,
} seriously. I can not comment about the laws in Australia, but I have a
very
} high regard for the regulations promulgated by the U. S. Dept. of
} Transportation (DOT) and IATA (for international shipments) as well as
the
} reasons behind them. And I can state, from first hand experience, that
if
} the regulators are presented with sound technical information for a
} reguation to be changed, they will indeed listen (at least in the US) and
} sometimes make changes.
}
} If anyone remains unconvinced, about the importance of adherence to all
} HAZMAT regulations, keep in mind that the ValuJet disaster was caused by
} someone not taking seriously these same regulations.
}
} While it is correct that one can incur almost unbelievalbly high shipping
} costs for HAZMATs, this is not always the case. In many instances, such
as
} for the ordering of osmium tetroxide, if one orders "smart", they can do
} their shipping at costs only nominally more than if the same weight of
} tweezers, grids, or SEM mounts were being shipped. Now this would not
apply
} for everything (e.g. our SPI Dusters, for example) but it does apply for
a
} surprising number of HAZMATs routinely used in EM labs. We are also in
} the process of reformulating some of our embedding kits so they too can
be
} shipped at the lower prices.
}
} We have tried to explain how a customer can "order smart" on our website,
} click on "Hazardous Items(Good News and Bad News)". While savings in
} shipping costs for domestic US customers are possible, the real
} beneficiaries are foreign customers now no longer are restricted to the
use
} of air freight (which has associated with it high minimum charges).
}
} And while we are on this subject, just remember, don't ever try to take
} HAZMATs in checked airline luggage to save some money, it is
unconscienable
} from a moral standpoint and puts at risk everyone on the plane flight.
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================




From: Peter Steele :      STEELEP-at-allkids.org
Date: Mon, 08 Sep 1997 09:16:15 -0400
Subject: TEM- skin biopsies pproblems -Reply

Contents Retrieved from Microscopy Listserver Archives
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You ma want to define and echo this question to the Society of
Ultrastructural Pathology's List Server at their web site
http://sup.ultrakohl.com.


} } } maria lucia ribeiro caldas {caldasml-at-amcham.com.br}
09/07/97 07:16pm } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America

I am having technical problems in EM skin biopsies. I would like
to have
a detailed protocol for processing skin biopsies.





From: Woody.N.White-at-mcdermott.com
Date: Mon, 8 Sep 1997 10:10:00 -0500
Subject: Re: More ? on EDS for Al-alloys

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I would never touch the Be window! If oil is present and you need
to clean... CAREFULLY drip solvent acrosse the face to rinse the
window. Be sure the solvent of choice not only will disolve the
oil, but NOT the materials used to construct the Be window.
Woody

{snip}

Let's go to the question: Have you ever cleaned the Be window of
your EDS system? If the answer is positive, how often? Or, just wake me up
---- NEVER EVER THINK ABOUT THAT, YOU THE TROUBLE MAKER!
I swear I have never been a trouble-maker. I just want to learn
something from you. Just talking, no action following.
It is too lang. Thank you for your time.

Charlie Kong
kong-at-t-rex.materials.unsw.edu.au




From: D. Reynolds :      subwiz-at-lostvegas.com
Date: Mon, 8 Sep 1997 07:27:25 -0700
Subject: Advertisement: FREE DOWNLOAD: Register Your Web Site On Over 590 Search Engines "INSTANTLY"

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From: bozzola-at-siu.edu (John J. Bozzola)
Date: Mon, 8 Sep 1997 09:58:20 -0600
Subject: Re: More ? on EDS for Al-alloys

Contents Retrieved from Microscopy Listserver Archives
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I enjoyed your discussion on x-ray analysis and the challenge one faces
from some students. As the saying goes, "you never really understand
something until you teach it." Students will forever keep us on our toes
and perhaps even force us to open certain doors that we might otherwise
have tip-toed by.

Anyway, you asked does one clean the detector window? Yes, we clean our
NORVAR window perhaps once a year or two - depending upon how dirty it has
become. Most software has a subroutine for checking the efficience of the
window using a standard of some sort (iron, for example). When efficiency
drops, then one should carefully clean the window. In our case, we clean
the window by removing the detector and slowly allowing 10-15 ml of
methanol to flow over the window. The methanol is not directed at the
window but onto the metal part of the housing about an inch away from the
window. The methanol then gently flows over the window and washes away most
of the oil. Check with your manufacturer, however, since different windows
will require different treatments and/or solvents. You must be very gentle
with the detector since the window and/or electronics will be damaged by
mishandling. Definitely talk to a service person before doing this - if you
are new to this procedure. We were, but now we feel comfortable cleaning
the window.


####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Neckers Building, Room 146 - B Wing
Southern Illinois University
Carbondale, IL 62901
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: Matthias Ochs :      mochs-at-gwdg.de
Date: Mon, 08 Sep 1997 17:16:07 +0200
Subject: Re: Morphometry

Contents Retrieved from Microscopy Listserver Archives
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Morphometry (i.e. measurement of form) in the biomedical field is
mostly done on thin sections either at the LM or the EM level.
The methods of choice for obtaining morphometric estimates from=20
sections are those of stereology.
The "classical" foundations of stereology including assumption-based
methods are covered in:

Weibel ER: Stereological Methods, Vol. 1+2, Academic Press, 1979/80

Modern design-based methods, mainly developed during the last 10-15
years, try to focus on the theory of unbiased sampling and on the
counting and sizing of particles as well as on the orientation of=20
structures in 3D space.
Reviews of modern stereological methods are e.g.:

Gundersen HJG et al.: APMIS 96;379-394 and 857-881 (1988)
Cruz-Orive LM, Weibel ER: Am J Physiol 258;L148-L156 (1990)
Mayhew TM: Exp Physiol 76;639-665 (1991)

The Journal of Microscopy and Acta Stereologica are the official journals
of the International Society for Stereology.

If you have any further questions please contact me directly.

Sincerely,

Matthias Ochs
Matthias Ochs, M.D.
Dept. of Anatomy
Div. of Electron Microscopy
University of G=F6ttingen
Kreuzbergring 36
D-37075 G=F6ttingen
Germany
Phone: +49 551 397036
Fax: +49 551 397004




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Mon, 08 Sep 1997 11:45:27 -0400
Subject: Ge-Sn polishing question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I'm forwarding this message for a colleague in my department. Please
resond to me at the address below. TIA.

Owen


} Seeking appropriate polishing procedures for Ge-Sn samples
} ----------------------------------------------------------
}
} I have eight Ge-Sn samples ( five Ge-10 at% Sn and three Ge-40 at% Sn )
} which have been annealed at 400 C, 500 C, 600 C, 700 C, and 800 C for five
} weeks. I need to have good polishing procedures so that good
} polished surfaces can be obtained in order to provide a detailed
microchemical
} analysis on these samples. Sn smearing problems arise when a fine grid,
for example,
} 0.05 micron alumina, was applied to the samples. This might due to the
} fact that we have both soft and hard materials in the samples.
}
} Any suggestion on how to polish these samples is highly appreciate. Thank
} you.

Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Mon, 8 Sep 1997 18:15:37 +0200 (MET DST)
Subject: Diehl

Contents Retrieved from Microscopy Listserver Archives
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A technical question:

Does anyone over there know the address (fax, phone, whatever...) of the
German maker of time programmators called DIEHL. We have been looking for
them all around talking with various vendors of electronics material, not
to avail. (if someone can tell me the www address of the phone numbers
searching tool of Deutsche Telekom or Chamber of Commerce in Germany that
could even be a hint).

Thanks,
Yves Maniette





From: Barbara Foster :      mme-at-mail.map.com
Date: Mon, 08 Sep 1997 12:27:57 -0700
Subject: Re: optical microscopy question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jennifer T. Morse wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.}
} I was recently given the following homework assignment: Actual
} resolution as determined by measuring the smallest distance between two
} distinguishable points in a suitable specimen (resolution standard), is
} usually greater than the linear resolution of the microscope. Describe
} the factors that contribute to this discrepency. Find and list suitable
} good references. Can you give me any help in solving this problem? Do
} you know of any good sources to speak to? Do you know the answer? Thank
} you for your help, Fred Meisenkothen

Jennifer & Fred,

Two of the best, upper level resources on this type of thing are:
1. Advanced Light Microscopy by Maksymilian Pluta (Elsevier - 3 volume
set; volume 1 = ISBM 0-444-98939-0)
2. Progress in Microscopy, M. Francon (Row, Peterson & Co./Pergamon
Press)

Born & Wolf's book on the Physics of Optics is also a good reference. I
have no info on publisher, etc.

Let me know what you find.

Best regards,
Barbara Foster
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com




From: Barbara Foster :      mme-at-mail.map.com
Date: Mon, 08 Sep 1997 12:42:19 -0700
Subject: Re: Morphometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

RE73-at-aol.com wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.}
} I am having trouble finding information concerning the theroy of morphometry
} and its benifits to the biological field... any sugjestions.

Suggest the following:
1. John Russ's Handbook (already sent to you via another email)
2. Image analysis in Biology, Donat-P Hader, ed; CRC Press
ISBN 0-8493-6033-1
3. Electronic Light Microscopy by David Shotton, ed., Wiley-Liss (ISBN
0-471-56077-4)

If you need just a quick overview of imaging principles, please see:
Optimizing Light Microscopy for Biological and Clinical Laboratories,
Kendall-Hunt (ISBN 0-7872-3538-5). We have them available, still on show
discount from the Microscopy & Microanalysis meeting.

Good luck!
Barbara Foster
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com




From: D. Reynolds :      subwiz-at-lostvegas.com
Date: Monday, September 08, 1997 11:46 AM
Subject: Advertisement: FREE DOWNLOAD: Register Your Web Site On Over 590

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----





From: DON_STEELE-at-CCKRDC.CA.ALCAN.CA
Date: Mon, 08 Sep 1997 16:09 -0400 (EDT)
Subject: JOB POSTING

Contents Retrieved from Microscopy Listserver Archives
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Your question is very broad so you will have to narrow the following list to
your interests. Enjoy the search!:

Bibliography

Aherne, W.A. and M.S. Dunnill 1982 Morphometry. Edward Arnold (Publishers)
Ltd. London,-205.

Bertram, J.F. and R.P. Bolender 1990 Counting cells with stereology: Random
versus serial sectioning. J Electron Microsc Techn, 14:32-38.

Bianciardi, G., P. Tanganelli, and G. Weber 1993 Blood cell activation: New
perspectives from ultrastructural morphometry. Semin Thromb Hemost,
19:108-114.

Bolender, R.P. 1978 Correlation of morphometry and stereology with
biochemical analysis of cell fractions. Int Rev Cytol, 55:247-289.

Bolender, R.P. 1981 Stereology: applications to pharmacology. Annu Rev
Pharmacol Toxicol, 21:549-573.

Bolender, R.P. 1982 Stereology and its uses in cell biology. Ann NY Acad
Sci, 383:1-16.

Bolender, R.P. 1992a Quantitative morphology for biologists and computer
scientists: I. Computer-aided tutorial for biological stereology (version
1.0). Microsc Res Techn, 21:338-346.

Bolender, R.P. 1992b Biological stereology: history, present state, future
directions. Microsc Res Techn, 21:255-261.

Cruz-Orive, L.-M. 1980 On the estimation of particle number. J Microsc,
120:15-27.

Cruz-Orive, L.-M. and E.R. Weibel 1981 Sampling designs for stereology. J
Microsc, 122:235-257.

Cruz-Orive, L.-M. 1987 Particle number can be estimated using a disector of
unknown thickness: the selector. J Microsc, 145:121-142.

Cruz-Orive, L.-M. and E.R. Weibel 1990 Recent stereological methods for cell
biology: a brief survey. Am J Physiol, 258:L148-56.

Dardick, I. and D. Caldwell 1985 Reproducibility of morphometric image
analysis. Hum Pathol, 16:1178

Freedman, L.S. 1974 A note on Aherne's method of counting tissue components
in relatively thick sections. J Microsc, 100:219-225.

Gundersen, H.J.G. 1977 Notes on the estimation of the numerical density of
arbitrary profiles: the edge effect. J Microsc, 111:219-223.

Gundersen, H.J.G., M. Boysen, and A. Reith 1981 Comparison of semiautomatic
digitizer-tablet and simple point counting performance in morphometry.
Virchows Arch B Cell Pathol, 37:317-325.

Gundersen, H.J.G. 1986 Stereology of arbitrary particles. A review of
unbiased number and size estimators and the presentation of some new ones,
in memory of William R. Thompson. J Microsc, 143:3-45.

Gundersen, H.J.G. and E.B. Jensen 1987 The efficiency of systematic sampling
in stereology and its prediction. J Microsc, 147(3):229-263.

Gundersen, H.J.G. 1988 The nucleator. J Microsc, 151:3-21.

Hammel, I., D. Lagunoff, M. Bauza, and E. Chi 1983 Periodic, multimodal
distribution of granule volumes in mast cells. Cell Tissue Res, 228:51-59.

Henderson, W.R. and E.Y. Chi 1985 Ultrastructural characterization and
morphometric analysis of human eosinophil degranulation. J Cell Sci,
73:33-48.

Loud, A.V., W.C. Barany, and B.A. Pack 1965 Quantitative evaluation of
cytoplasmic structures in electron micrographs. Lab Invest, 14:258-270.

Loud, A.V. and P. Anversa 1984 Biology of disease: Morphometric analysis of
biologic processes. Lab Invest, 50:250-261.

Loud, A.V. 1987 Electron microscopic morphometry. Anal Quant Cytol Histol,
9:7-12.

Maser, M.D. 1990 An overview of morphometry and stereology. Tx A&M Univ EM
Views, Issue No. 4:3-8,15-16.

Mathieu, O., L.-M. Cruz-Orive, H. Hoppeler, and E.R. Weibel 1981 Measuring
error and sampling variation in stereology: comparison of the efficiency of
various methods for planar image analysis. J Microsc, 121:75-88.

Mayhew, T.M. and M.A. Williams 1971 A comparison of two sampling procedures
for stereological analysis of cell pellets. J Microsc, 94:195-204.

Mayhew, T.M. and L.-M. Cruz 1973 Stereological correction procedures for
estimating true volume proportions from biased samples. J Microsc,
99:287-299.

Mayhew, T.M. and L.-M. Cruz-Orive 1974 Caveat on the use of the Delesse
principle of areal analysis for estimating component volume densities. J
Microsc, 102:195-207.

Mayhew, T.M. 1979 Basic stereological relationships for quantitative
microscopical anatomy - a simple systematic approach. J Anat, 129:95-105.

Mayhew, T.M. and F.H. White 1980 Ultrastructural morphometry of isolated
cells: methods, models and applications. Pathol Res Pract, 166:239-259.

Mayhew, T.M. 1981 On the relative efficiencies of alternative ratio
estimators for morphometric analysis of cell membrane surface features. J
Microsc, 122:7-14.

Nazeran, H., F. Rice, W. Moran, and J. Skinner 1995 Biomedical image
processing in pathology: a review. Australas Phys Eng Sci Med, 18:26-38.

Peachey, L.D. 1982 A simple digital morphometry system for electron
microscopy. Ultramicrosc, 8:253-262.

Pesce, C.M. 1985 Morphometric studies need review by statisticians. Am J
Clin Pathol, 83:258

Pesce, C.M. 1987 Biology of disease. Defining and interpreting diseases
through morphometry. Lab Invest, 56:568-575.

Pitha, J.V. 1985 Computer-assisted planimetry. Hum Pathol, 16:1284-1285.

Schmid-Sch”nbein, G.W. and S. Chien 1989 Morphometry of human leukocyte
granules. Biorheol, 26:331-343.

Sokal, R.R. and F.J. Rohlf 1981 Biometry: the principles and practice of
statistics in biological research. Freeman, San Francisco,

Sokol, R.J., G. Hudson, J. Wales, and N.T. James 1988 Morphometry of human
blood leukocyte ultrastructure: Its potential value in haematology.
Haematol, 21:129-139.

Sokol, R.J., G. Hudson, J. Wales, and N.T. James 1991 Ultrastructural
morphometry of human leucocytes in health and disease. Electron Microsc Rev,
4:179-195.

Sterio, D.C. 1986 The unbiased estimation of number and sizes of arbitary
particles using the disector. J Microsc, 134:127-136.

Stoeber, W. 1965 Statistical size distribution analysis. Lab Invest,
14:892-908.

Underwood, E.E. 1970 Quantitative stereology. Addison-Wesley, Reading,

Vedel Jensen, E.B. and H.J.G. Gundersen 1992 The rotator. J Microsc,
170:35-44.

Webster, P. and G. Griffiths 1994 A novel method for mean cell volume
estimation. J Microsc, 174:85-92.

Weibel, E.R., G.S. Kistler, and W.F. Scherle 1966 Practical stereological
methods for morphometric cytology. J Cell Biol, 30:23-38.

Weibel, E.R. 1969 Stereological principles for morphometry in electron
microscopic cytology. Int Rev Cytol, 26:235-302.

Weibel, E.R. 1972 The value of stereology in analysing structure and
function of cells and organs. J Microsc, 95:3-13.

Weibel, E.R. and R.P. Bolender 1973 Stereological techniques for electron
microscopic morphometry. In: Principles and techniques of electron
microscopy: Biological applications. Vol. 3. M.A. Hayat, ed. Van Nostrand
Reinhold Co. New York, pp. 237-296.

Weibel, E.R. 1974 Selection of the best method in stereology. J Microsc,
100:261-269.

Weibel, E.R. 1975 Quantitation in morphology: possibilities and limits.
Beitr Pathol, 155:1-17.

Weibel, E.R. 1981 Stereological methods in cell biology: where are we--where
are we going? J Histochem Cytochem, 29:1043-1052.

Weibel, E.R. 1982 Biomorphometry in physiological and pathological research.
Acta Med Pol, 23:115-125.

Weibel, E.R. 1989 Measuring through the microscope: development and
evolution of stereological methods. J Microsc, 155:393-403.

Weibel, E.R. 1991 Fractal geometry: a design principle for living organisms.
Am J Physiol, 261:L361-9.


-------------------------------------
Name: Charles Gilbert VOC:(704)355-5261
Carolinas Medical Center FAX:(704)355-8424
Dept of Pediatric Research
PO Box 32861
Charlotte, NC 28232-2861



Alcan International Limited, Kingston Research and Development Centre,
is seeking a Materials Characterization Technologist to work in the
area of metallography and electron optics (SEM, TEM, Electron
Microprobe) support.

You will be involved in a wide range of activities aimed primarily at:
(a) characterizing the microstructures of Alcan's automotive and
packaging alloys which, in turn, support the Company's product and
process development work, and (b) determining the factors which
influence the performance of Alcan's sheet products. You will also
play a key role in the ongoing development of the laboratory's
materials characterization techniques.

We are seeking an individual skilled in metallographic specimen
preparation and the operation and maintenance of both optical and
electron microscopes. As the perfect candidate, you have a college
diploma or university degree in a materials science discipline, or the
equivalent, coupled with experience in both optical and electron
metallography and in the use of PC-based data acquisition and analysis
software. In addition, you must have the ability to cope with
numerous activities simultaneously and adapt to changing priorities.

We offer an excellent compensation package commensurate with
experience and a first-class working environment.

Interested candidates may send a resume by 18 September 1997 to:
Personnel Administrator, Alcan International Limited, Kingston
Research and Development Centre, Box 8400, Kingston, Ontario K7L 5L9.
Fax: (613) 541-2308

We are an equal opportunity employer.





From: Tedpel-at-aol.com
Date: Mon, 8 Sep 1997 17:03:58 -0400 (EDT)
Subject: Imaging nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

9/8/97

Charles Garber of SPI, Inc. wrote:

".....CdS nano-particles, at least the ones we have seen, those that would be
thin enough to "see" through, are smaller than the smallest holes we have
ever seen anyone able to make in a "lacy" film. So I think that there might
have been some misinformation accidently posted about this awhile back (see
Aug. 11 posting DUNNTEM-at-aol.com working for Ted Pella, Inc.) If I am wrong
about this please correct me and set the record straight."

Our posting on this subject stated that nono-particles have a tendency to
adhere to the inner surface of the holes in the lacey films - an ideal
location for EM imaging. Obviously, since the holes in the lacey film are
usually from 1 micron up in diameter, a 5nm particle will not lie across a
hole.


Charles Garber went on to say:

"This simple reality is often times missed by people worried about film
thickness (which in fact ought to not even be relavent in the case of a lacey
or holey film, because after all, you are getting your information through
the holes, not through the "lacy" areas). So the whole need to worry about
"thickness" per se really ought to be, in the case of lacey films, a
non-issue!"

We made no reference to the thickness of the lacey film. Obviously it is a
"non-issue". In fact, we do not see that anyone brought that subject up
except Charles Garber.

We feel that it is generous of the creator of this list to allow vendors to
post information which includes the products they have that might solve a
technical problem. It is, we think, misuse of the list to suggest that a
vendor is posting misinformation or to promote one's own products by
criticizing another's products.

Let's keep competitive sales techniques out of the microscopy list.

Ted Pella, Inc.





From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Mon, 8 Sep 1997 17:14:12 -0400 (EDT)
Subject: Philips CM Power Supply Problem

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To all CM users:


We have a CM12 TEM/STEM that is shut down at the moment because the +24
volt supply (located in the remote rack) does not want to start up. This
causes the microscope to shut down immediately after you press the main
power button. Remote bench testing of this supply with all the safety
circuits in place does not help in our diagnosis. We checked the setup
with the +5 volt supply, which is almost identical and it works.

If anyone has had similiar problems to the startup sequence
of this supply which prevents the microscope from powering up
I would be very interested in an email message from you.
ie. before we spend $4500 (Can) for a new/rebuilt supply from Philips.

email directly if you desire: eoptics-at-mcmaster.ca

Thanks in advance

Fred

********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1

********************************************************





From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 9 Sep 1997 09:37:27 GMT+1200
Subject: Re: More ? on EDS for Al-alloys

Contents Retrieved from Microscopy Listserver Archives
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Charlie:

} Several experts have advised me that we should give up the
} dependence upon the standardless analysis of EDS, although the others
} showed strong evidence to prove that it is working well.

Yes, but why not standardize? You are lucky to have a couple of
extremely good EDS systems, give them a chance to work
properly.


} My former colleague, Bruce, suggested that oily detector window
} might cause the problem for softer radiation such as Al-Ka. This message
} struck a spark in my poor memory. I really saw somebody who dared to wipe
} oil off the thin Be-window of a TN-5400 EDS using a cotton ball with a
} drop of acetone. That was ten years ago.
} Let's go to the question: Have you ever cleaned the Be window of
} your EDS system?

I use EDS for quantitative analysis of minerals, I get results which
are comparable to WDS (provocative statement, which I'm happy to
defend), I have an old probe with a fairly dirty vacuum system, and I
clean my window when the response to Na drops by about 50%, which
takes about 6 months. Al drops, too, but not so much.
I clean it by gently dribbling a stream of Freon over the whole end
of the detector so that the stream doesn't play onto the window
directly, but runs down over it.
I use Freon because it is a wonderful solvent for oil but is pretty
non-aggressive towards epoxies etc which may be used in the
construction of the window.
I wouldn't use acetone, too aggressive. When I run out of Freon I'll
use light petroleum spirit ("ligroin", "petroleum ether").

And DO NOT physically touch the window with ANYTHING at all!!!!!!!!!

But why don't you just follow the recommendations of your detector
manufacturer?

In fact, this leads me to a question which often comes to mind when I
read some of the postings --- they contain questions which could so
easily be answered by the manufacturer, such as the one a couple of
days ago about sources of replacement detector windows.

Am I just lucky in the quality of support that I get from Oxford
Australia (take a bow, Keith, Julie, and John, for an unsolicited
testimonial and thank-you)?

cheers

Ritchie

Ritchie Sims phone: 64 9 3737599 ext 7713
Department of Geology fax: 64 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Barbara Foster :      mme-at-map.com
Date: Mon, 08 Sep 1997 18:14:50 -0700
Subject: Re: optical microscopy question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Barbara Foster wrote:
}
} Jennifer T. Morse wrote:
} }
} } ------------------------------------------------------------------------}
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } -----------------------------------------------------------------------.} } } I was recently given the following homework assignment: Actual
} } resolution as determined by measuring the smallest distance between two
} } distinguishable points in a suitable specimen (resolution standard), is
} } usually greater than the linear resolution of the microscope. Describe
} } the factors that contribute to this discrepency. Find and list suitable
} } good references. Can you give me any help in solving this problem? Do
} } you know of any good sources to speak to? Do you know the answer? Thank
} } you for your help, Fred Meisenkothen
}
} Jennifer & Fred,
}
} Two of the best, upper level resources on this type of thing are:
} 1. Advanced Light Microscopy by Maksymilian Pluta (Elsevier - 3 volume
} set; volume 1 = ISBM 0-444-98939-0)
} 2. Progress in Microscopy, M. Francon (Row, Peterson & Co./Pergamon
} Press)
}
} Born & Wolf's book on the Physics of Optics is also a good reference. I
} have no info on publisher, etc.
}
} Let me know what you find.
}
} Best regards,
} Barbara Foster
}

--
ĐÏࡱá




From: Marc Benvenuto :      hydrox-at-jagunet.com
Date: Mon, 08 Sep 1997 19:42:57 +0000
Subject: 100% Prism

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking to convert an old Nikon Diaphot TMD with a 80/20 side
prism, to a 100/100% side port output. All that is involved is changing
the side prism. We have the 80/20 kind, which we would gladly exchange
at our expense with anyone in possesion of the 100/100 prism.

If interested please contact me to arrange the particulars.

Marc
JHU
Baltimore, MD




From: John Best :      jbest-at-vicon.net
Date: Mon, 08 Sep 1997 23:22:33 -0700
Subject: Shareware for Microscopists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All...........

Two requests.
Firstly, would anyone know of a how to guide, including download sites
for bringing spectra files from a TN-5500 into a PC? We need cabling,
some sort of FTP on both sides and hopefully a spectra manipulation
program. I can't help but think there are hundreds of microscopists who
would find this useful.

Secondly, I am assembling a list of shareware useful to microscopists. I
would like to assemble a list of programs, descriptions and download
sites, which will be posted to the listserver (or sent out directly to
anyone who requests it, if it gets too long). Should replies to this
second request be posted, or come to me directly? I think if the
shareware is generally useful to microscopists, a brief description,
review and URL would be appropriate material for the listserver. How
about it Nestor?

Cheers all.............
--
John Best
ELMDAS Co.




From: ellis, sarah :      sarahe-at-raid.res.petermac.unimelb.edu.au
Date: Tue, 9 Sep 1997 16:43:38 +1000
Subject: Collecting and fixing yeast for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Friends,

I am send to you information from "Morphology Digest" 1997, issue 5, from
8 september 1997 (Nl) about creation new sterelogy list in Calgary.
I think that will be interesting for all.



---------- Forwarded message ----------

I have to prepare some yeast for SEM. The person who requested this
work wants some pretty pictures of his yeast, Schizosaccharomyces pombe,
for use in seminars. I have read up on some techniques to use but have
two main queries:
1. To collect the yeast onto filter paper ( a method used in
several publications), do I just drop a suspension of the yeast onto the
filter paper? What sort of filter paper do I use? Is there a "better"
way of collecting these cells such as settling them onto poly-l-lysine
coverslips?
2. Is there a preferred fixative that works? The literature
suggests a plethora of fixative cocktails! For TEM, I slam the cells
onto a liquid nitrogen cooled copper mirror and process them via
substitution in methanol and embed them in lowicryl HM20.
We do not have an ESEM so please don't suggest I view them unfixed.
Thanking you all in advance, this really is a great way of learning and
sharing information!
Sarah Ellis




From: Marti, Jordi :      MartiJ-at-MTOMP201.Research.Allied.com
Date: Tue, 09 Sep 97 08:34:00 EDT
Subject: on SPM

Contents Retrieved from Microscopy Listserver Archives
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We are interested in measuring the thermal conductivity of micron and
submicron phases in a composite.Could any one tell me if SPM (Scanning
Probe Microscopy) can be used to measure thermal conductivity and if this
is a quantitative or purely qualitative technique ? Are the results relative
or absolute ?

Thanks

Jordi Marti





From: James W. Larkin :      jamesl-at-healthtech.com (by way of Nestor J.
Date: Tue, 9 Sep 1997 07:56:16 -0500
Subject: Advances in Cellular Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Your question is very broad so you will have to narrow the following list to
your interests. Enjoy the search!:

Bibliography

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Ltd. London,-205.

Bertram, J.F. and R.P. Bolender 1990 Counting cells with stereology: Random
versus serial sectioning. J Electron Microsc Techn, 14:32-38.

Bianciardi, G., P. Tanganelli, and G. Weber 1993 Blood cell activation: New
perspectives from ultrastructural morphometry. Semin Thromb Hemost,
19:108-114.

Bolender, R.P. 1978 Correlation of morphometry and stereology with
biochemical analysis of cell fractions. Int Rev Cytol, 55:247-289.

Bolender, R.P. 1981 Stereology: applications to pharmacology. Annu Rev
Pharmacol Toxicol, 21:549-573.

Bolender, R.P. 1982 Stereology and its uses in cell biology. Ann NY Acad
Sci, 383:1-16.

Bolender, R.P. 1992a Quantitative morphology for biologists and computer
scientists: I. Computer-aided tutorial for biological stereology (version
1.0). Microsc Res Techn, 21:338-346.

Bolender, R.P. 1992b Biological stereology: history, present state, future
directions. Microsc Res Techn, 21:255-261.

Cruz-Orive, L.-M. 1980 On the estimation of particle number. J Microsc,
120:15-27.

Cruz-Orive, L.-M. and E.R. Weibel 1981 Sampling designs for stereology. J
Microsc, 122:235-257.

Cruz-Orive, L.-M. 1987 Particle number can be estimated using a disector of
unknown thickness: the selector. J Microsc, 145:121-142.

Cruz-Orive, L.-M. and E.R. Weibel 1990 Recent stereological methods for cell
biology: a brief survey. Am J Physiol, 258:L148-56.

Dardick, I. and D. Caldwell 1985 Reproducibility of morphometric image
analysis. Hum Pathol, 16:1178

Freedman, L.S. 1974 A note on Aherne's method of counting tissue components
in relatively thick sections. J Microsc, 100:219-225.

Gundersen, H.J.G. 1977 Notes on the estimation of the numerical density of
arbitrary profiles: the edge effect. J Microsc, 111:219-223.

Gundersen, H.J.G., M. Boysen, and A. Reith 1981 Comparison of semiautomatic
digitizer-tablet and simple point counting performance in morphometry.
Virchows Arch B Cell Pathol, 37:317-325.

Gundersen, H.J.G. 1986 Stereology of arbitrary particles. A review of
unbiased number and size estimators and the presentation of some new ones,
in memory of William R. Thompson. J Microsc, 143:3-45.

Gundersen, H.J.G. and E.B. Jensen 1987 The efficiency of systematic sampling

in stereology and its prediction. J Microsc, 147(3):229-263.

Gundersen, H.J.G. 1988 The nucleator. J Microsc, 151:3-21.

Hammel, I., D. Lagunoff, M. Bauza, and E. Chi 1983 Periodic, multimodal
distribution of granule volumes in mast cells. Cell Tissue Res, 228:51-59.

Henderson, W.R. and E.Y. Chi 1985 Ultrastructural characterization and
morphometric analysis of human eosinophil degranulation. J Cell Sci,
73:33-48.

Loud, A.V., W.C. Barany, and B.A. Pack 1965 Quantitative evaluation of
cytoplasmic structures in electron micrographs. Lab Invest, 14:258-270.

Loud, A.V. and P. Anversa 1984 Biology of disease: Morphometric analysis of
biologic processes. Lab Invest, 50:250-261.

Loud, A.V. 1987 Electron microscopic morphometry. Anal Quant Cytol Histol,
9:7-12.

Maser, M.D. 1990 An overview of morphometry and stereology. Tx A&M Univ EM
Views, Issue No. 4:3-8,15-16.

Mathieu, O., L.-M. Cruz-Orive, H. Hoppeler, and E.R. Weibel 1981 Measuring
error and sampling variation in stereology: comparison of the efficiency of
various methods for planar image analysis. J Microsc, 121:75-88.

Mayhew, T.M. and M.A. Williams 1971 A comparison of two sampling procedures
for stereological analysis of cell pellets. J Microsc, 94:195-204.

Mayhew, T.M. and L.-M. Cruz 1973 Stereological correction procedures for
estimating true volume proportions from biased samples. J Microsc,
99:287-299.

Mayhew, T.M. and L.-M. Cruz-Orive 1974 Caveat on the use of the Delesse
principle of areal analysis for estimating component volume densities. J
Microsc, 102:195-207.

Mayhew, T.M. 1979 Basic stereological relationships for quantitative
microscopical anatomy - a simple systematic approach. J Anat, 129:95-105.

Mayhew, T.M. and F.H. White 1980 Ultrastructural morphometry of isolated
cells: methods, models and applications. Pathol Res Pract, 166:239-259.

Mayhew, T.M. 1981 On the relative efficiencies of alternative ratio
estimators for morphometric analysis of cell membrane surface features. J
Microsc, 122:7-14.

Nazeran, H., F. Rice, W. Moran, and J. Skinner 1995 Biomedical image
processing in pathology: a review. Australas Phys Eng Sci Med, 18:26-38.

Peachey, L.D. 1982 A simple digital morphometry system for electron
microscopy. Ultramicrosc, 8:253-262.

Pesce, C.M. 1985 Morphometric studies need review by statisticians. Am J
Clin Pathol, 83:258

Pesce, C.M. 1987 Biology of disease. Defining and interpreting diseases
through morphometry. Lab Invest, 56:568-575.

Pitha, J.V. 1985 Computer-assisted planimetry. Hum Pathol, 16:1284-1285.

Schmid-Sch”nbein, G.W. and S. Chien 1989 Morphometry of human leukocyte
granules. Biorheol, 26:331-343.

Sokal, R.R. and F.J. Rohlf 1981 Biometry: the principles and practice of
statistics in biological research. Freeman, San Francisco,

Sokol, R.J., G. Hudson, J. Wales, and N.T. James 1988 Morphometry of human
blood leukocyte ultrastructure: Its potential value in haematology.
Haematol, 21:129-139.

Sokol, R.J., G. Hudson, J. Wales, and N.T. James 1991 Ultrastructural
morphometry of human leucocytes in health and disease. Electron Microsc Rev,
4:179-195.

Sterio, D.C. 1986 The unbiased estimation of number and sizes of arbitary
particles using the disector. J Microsc, 134:127-136.

Stoeber, W. 1965 Statistical size distribution analysis. Lab Invest,
14:892-908.

Underwood, E.E. 1970 Quantitative stereology. Addison-Wesley, Reading,

Vedel Jensen, E.B. and H.J.G. Gundersen 1992 The rotator. J Microsc,
170:35-44.

Webster, P. and G. Griffiths 1994 A novel method for mean cell volume
estimation. J Microsc, 174:85-92.

Weibel, E.R., G.S. Kistler, and W.F. Scherle 1966 Practical stereological
methods for morphometric cytology. J Cell Biol, 30:23-38.

Weibel, E.R. 1969 Stereological principles for morphometry in electron
microscopic cytology. Int Rev Cytol, 26:235-302.

Weibel, E.R. 1972 The value of stereology in analysing structure and
function of cells and organs. J Microsc, 95:3-13.

Weibel, E.R. and R.P. Bolender 1973 Stereological techniques for electron
microscopic morphometry. In: Principles and techniques of electron
microscopy: Biological applications. Vol. 3. M.A. Hayat, ed. Van Nostrand
Reinhold Co. New York, pp. 237-296.

Weibel, E.R. 1974 Selection of the best method in stereology. J Microsc,
100:261-269.

Weibel, E.R. 1975 Quantitation in morphology: possibilities and limits.
Beitr Pathol, 155:1-17.

Weibel, E.R. 1981 Stereological methods in cell biology: where are we--where
are we going? J Histochem Cytochem, 29:1043-1052.

Weibel, E.R. 1982 Biomorphometry in physiological and pathological research.

Acta Med Pol, 23:115-125.

Weibel, E.R. 1989 Measuring through the microscope: development and
evolution of stereological methods. J Microsc, 155:393-403.

Weibel, E.R. 1991 Fractal geometry: a design principle for living organisms.
Am J Physiol, 261:L361-9.


-------------------------------------
Name: Charles Gilbert VOC:(704)355-5261
Carolinas Medical Center FAX:(704)355-8424
Dept of Pediatric Research
PO Box 32861
Charlotte, NC 28232-2861


Cambridge Healthtech Institute's
Advances in Cellular Imaging
November 13-14, 1997
Westin Hotel Horton Plaza
San Diego, California

TECHNICAL TRENDS AND ADVANCES
Multiphoton Excitation Imaging and Photochemistry in Cells
and Tissue
Dr. Warren Zipfel, Cornell University
Highly Resolved Cell and Tissue Optical Imaging in Real Time
Dr. Daniel Farkas, Carnegie Mellon University
Combined Fluorescent and Gold Cluster Probes: "Simultaneous"
Labeling for Light and Electron Microscopy
Dr. Richard Powell, NANOPROBES, Inc.
Novel Magnetic Messenger Labeling System
Mr. Lonnie Adelman, Ericomp Inc.

VIEWING REAL-TIME CELLULAR CHANGES
Imaging Drug Uptake and Metabolism in Living Intestinal Tissue
with Confocal and Two-Photon Microscopy
Dr. Marshall Montrose, Johns Hopkins University School
of Medicine
Dynamic Changes in Intracellular pH and Ca2+
Dr Randi Silver, Cornell University Medical College (invited)
Smart Magnetic Resonance Contrast Agents: A New Generation of
Image Enhancement Media
Dr. Tom Meade, California Institute of Technology
Multiple Fluorescent Proteins and Fluorescence Microscopy to Monitor
Live Cell Activity or Screen for Protein Localization
Dr. Neal Gliksman, Universal Imaging Corporation
Multiphoton Laser Scanning Fluorescence Microscopy
Dr. Victoria Centonze Frohlich, University of Wisconsin-
Madison

IMAGE ANALYSIS AND INTERPRETATION
Quantitative Molecular Image Analysis
Dr. Branko Palcic, British Columbia Cancer Research
Centre (invited)
Image Analysis Tools
Dr. Paul Goodwin, Fred Hutchinson Cancer Research
Institute (invited)
Quantitative Automated Microscopy
Dr. Frans Nauwelaers, Becton Dickinson Cellular Imaging
Systems (invited)
Fluorescence Imaging MicroSpectrophotometer (FIMS)
Dr. Douglas Youvan, KAIROS Scientific Inc.

SCREENING AND DRUG DEVELOPMENT
High-Content, Cell-Based Screening: Easing the Bottlenecks of
Target Validation and Optimization of Lead Compounds
Dr. Kenneth Giuliano, BioDx, Inc.
Applications of the Fluorometric Imaging Plate Reader (FLIPR)
Technology to High-Throughput Screening
Dr. Simon Pitchford, Molecular Devices Corporation
Use of Fluorescence Polarization in Drug Screening
Dr. Michael Jolley, Jolley Consulting and Research Inc.
(invited)
Fluorescence-Based Screening of Cellular Changes in Ion
Concentrations for Drug Development
Dr. Carla Suto, SIBIA Neuroscience, Inc. (invited)
Imaging Requirements for Faster Drug Development: Screening with
Higher Density Formats
Dr. Al Kolb, Packard Instrument Company (tentative)

Improved technology for imaging of cells and related targets is
having a dramatic impact on pharmaceutical research and development,
driven in part by the demand for greater speed, precision, and
automation. Novel strategies for labels, better software for image
enhancement and analysis, and progress integrating imaging with
other laboratory functions are being applied to a growing range of
applications. Advantages in such diverse segments as microscopy,
cytology, and cellular analysis, as well as more applied uses such as
assessment of gels and drug development screening, will be
discussed. All of these activities share a similar goal of rapidly
and correctly translating images into data that can be stored, used,
compared, and manipulated with as much ease and as little human
intervention as possible. These developments promise to have a
dramatic impact on laboratory productivity, and any research manager
involved in these segments should consider participating.

HOTEL INFORMATION
Westin Hotel Horton Plaza Reservations made after the cut-off
910 Broadway Circle date will be accepted on a space and
San Diego, CA 92101 rate availability basis. Available
rooms are limited, so please book
T: 619-239-2200 early.
F: 619-239-0509 Please identify yourself as a
Cut-off Date: October 30, 1997 Cambridge Healthtech Institute
Room Rate: $135 Single/Double conference attendee to receive the
reduced room rate.

TRAVEL INFORMATION
TRAVELWORLD T: 717-288-9311 or 800-828-6033
601 Market Street F: 717-288-4693
Kingston, PA 18704

Exclusive airline discounts are available on American Airlines as
well as other specific airlines when tickets are purchased through
TRAVELWORLD at least 14 days prior to the meeting date. Some
restrictions apply.

CALL FOR POSTERS
Cambridge Healthtech Institute encourages attendees to gain further
exposure by presenting their work in the poster sessions. Please fill
out the registration form, with the poster title and primary author.
To ensure inclusion in the conference binder, a one-page summary must
be submitted by October 3, 1997.

CALL FOR EXHIBITORS
Space is available for companies interested in exhibiting products
and services related to cellular imaging. This meeting should attract
up to several hundred senior researchers and managers representing a
broad range of disciplines and perspectives. Please contact Jim MacNeil
of Cambridge Healthtech Institute at 617-630-1341 to obtain an exhibitor
package or to inquire about offering a workshop during the meeting. Exhibit
space is limited so call now to reserve a space at this premier event.

Each registration includes all conference sessions, posters and
exhibits, one luncheon and reception, continental breakfasts, all
refreshment breaks, and a copy of the document binder.

Handicapped Equal Access: In accordance with the ADA, Cambridge
Healthtech Institute is pleased to arrange for special accommodations
for attendees with special needs. All requests for such assistance
must be submitted in writing to CHI at least 30 days prior to the
start of the meeting.

Substitution/Cancellation Policy
In the event that you need to cancel a registration you may:
Transfer your registration to a colleague within your
organization.
Credit your registration to another Cambridge Healthtech
Institute program.
Request a refund minus a $75 processing fee.
Request a refund minus the cost ($150) of ordering a copy
of the document binder.
Cancellations will only be accepted up to one week prior to the
conference.

Program and speakers are subject to change.

------------------------Cut and Print Here------------------------

Yes!|__| Please register me for Advances in Cellular Imaging 571E

Advance Registration (by October 3, 1997)
|__| $795 Commercial
|__| $395 Academic, Government, Hospital-Affiliated
On-site or Late Registration (after October 3, 1997)
|__| $895 Commercial
|__| $445 Academic, Government, Hospital-Affiliated
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|__| I am interested in presenting a poster at ADVANCES IN CELLULAR
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FAX or MAIL your reservation/registration to:
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http:www.healthtech.com/conferences/






From: EvexAnalyt-at-aol.com
Date: Tue, 9 Sep 1997 09:25:22 -0400 (EDT)
Subject: Re: Shareware for Microscopists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In a message dated 97-09-09 03:38:32 EDT, jbest-at-vicon.net (John Best) writes:

{ { Firstly, would anyone know of a how to guide, including download sites
for bringing spectra files from a TN-5500 into a PC? We need cabling,
some sort of FTP on both sides and hopefully a spectra manipulation
program. I can't help but think there are hundreds of microscopists who
would find this useful. } }


Greeting John,

We have just the program for you.

The software is called VIDX X-ray Microanalysis. It is a Windows 95 and NT
software. (It is regularly sold to function with our PC based X-ray
microanalysis and digital Imaging hardware)

The software will open fthe most popular file formats. We can perform both
offline and on-line connections to many older vintage X-ray analyzers such as
Edax, Kevex, Link, Noran, PGT, Tracor. That means you don't have to go and
buy a brand new x-ray analyzer.

Prices are affordable.


For more information contact us at

Evex Analytical
857 State Road
Princeton, NJ 08540
609-252-9192

www.evex.com




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 9 Sep 1997 08:47:41 -0600
Subject: Re: Collecting and fixing yeast for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sarah,

Collect the yeast on to membrane filters with nice circular pores (e.g.
Nucleopore), not a torturous-path filter like filter paper (or e.g.
Millipore). This will give a nice smooth background against which to view
the yeast. Filters with 0.22 micron holes are maybe best, although 0.45
micron will work. The pores will also give an *approximate!* size standard
for the yeast cells.

*Before* collecting the yeast, coat both sides of the filters in a sputter
coater. This gives better conductivity for viewing. If you're rich, use
silver filters instead.

For fixation, I'd use the recipe the article(s) that show SEMs of yeast
most like what you're trying to achieve (including 'scope kV), and is the
simplest.

Phil

} I have to prepare some yeast for SEM. The person who requested this
} work wants some pretty pictures of his yeast, Schizosaccharomyces pombe,
} for use in seminars. I have read up on some techniques to use but have
} two main queries:
} 1. To collect the yeast onto filter paper ( a method used in
} several publications), do I just drop a suspension of the yeast onto the
} filter paper? What sort of filter paper do I use? Is there a "better"
} way of collecting these cells such as settling them onto poly-l-lysine
} coverslips?
} 2. Is there a preferred fixative that works? The literature
} suggests a plethora of fixative cocktails! For TEM, I slam the cells
} onto a liquid nitrogen cooled copper mirror and process them via
} substitution in methanol and embed them in lowicryl HM20.
} We do not have an ESEM so please don't suggest I view them unfixed.
} Thanking you all in advance, this really is a great way of learning and
} sharing information!
} Sarah Ellis

}}}}}}}}}}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{{{{{{{{{{

Philip Oshel
Station A
PO Box 5037
Champaign, IL 61825-5037
(217) 355-1143
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again *****







From: Ming Chen :      mingchen-at-gpu.srv.ualberta.ca
Date: Tue, 9 Sep 1997 08:45:48 -0600 (MDT)
Subject: Re: Collecting and fixing yeast for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Tue, 9 Sep 1997, ellis, sarah wrote:

} 1. To collect the yeast onto filter paper ( a method used in
} several publications), do I just drop a suspension of the yeast onto the
} filter paper? What sort of filter paper do I use? Is there a "better"
} way of collecting these cells such as settling them onto poly-l-lysine
} coverslips?

You can drop the cells onto poly-l-lysine coated coverslips after osmium
fixation. Then process it as usual for dehydration and critical point dry.

Best regards,

***********************************************
* Ming H. Chen, PhD *
* Medicine/Dentistry Electron Microscopy Unit *
* University Of Alberta. *
* Edmonton, Alberta, Canada *
* *
* Visit My Page At: *
* http://www.ualberta.ca/~mingchen *
***********************************************








From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Tue, 9 Sep 1997 17:05:33 +-200
Subject: WANTED: Used REICHERT KF 80

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone

Does anyone out there know of a REICHERT KF 80 Cryofixation system for sale? Kindly reply directly to me.

Thank you.



James Wesley-Smith
Electron Microscope Unit
University of Natal
Durban, South Africa





From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 9 Sep 1997 08:24:18 -0700 (PDT)
Subject: Re: TEM- skin biopsies pproblems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello, Here is info on our procedures. We are a "Skin LAB".

PROCESSING OF SKIN TISSUE FOR LIGHT AND ELECTRON MICROSCOPY
EMBEDDING IN EPON 812
1. Place newly received tissue* in 1/2 Karnovskys fixative overnight.
2. Take EPON 812 mixture out of refrigerator (let it sit under the hood
for at least 2 hours before opening the container). This mixture is a
combination of DDSA, NMA, and EMBED 812.
(48% of 812, 31% of DDSA, 21% of NMA)
3. Rinse biopsy in 0.1 M sodium cacodylate buffer x2, 15 minutes each
rinse.
4. Post-fix in 1% osmium tetroxide, 1 and 1/2 hours. (mix 1:1, 2% OsO4
with 0.2 M sodium cacodylate buffer).
5. Rinse in dH2O x 2, 15 minutes each rinse.
6. En-bloc stain with 1% Uranyl Acetate for 1 and 1/2 hours.
7. Dehydrate through an ascending ETOH series
35% x2 (15 minutes each)
70% x2 (15 minutes each)
95% x2 (15 minutes each)
100% x2 (30 minutes each)
8. Add the catalyst to the EPON 812 mixture (.2ml of DMP30 per 10 ml
resin).
Stir slowly for 10 minutes.
9. Clear biopsy in Propylene oxide x2, 15 minutes each rinse.
10. Infiltrate by placing biopsy into a
3:1 mixture of Propylene oxide:EPON for 3-4 hours
2:1 mixture for 12-16 hours (overnight) with caps off
1:1 mixture for 12-16 hours (overnight) with caps off
11. Place biopsy into an embedding mold with fresh 100% EPON for 8 hours,
then place mold into 60 oC oven for curing (24-48 hours).

EPON 812 can be substituted with either EMBED 812 (EMS), PolyBed 812
(Polysciences), Medcast or Eponate 12 (Ted Pella). All have the
ingredients DDSA, NMA, and DMP 30.

EMBEDDING PROCEDURE FOR SKIN BIOPSIES

ROUTINE RAPID

1/2 KARNOVSKYS overnight 2hr to
overnight

0.1M NaCaco 15minx2 5-10min
1%OsO4/.1M Na Caco 11/2 hrs 20min

dH2O 15minx2 3-5min

1%UA 11/2 hrs 20min

35%ETOH 15minx2 50% 5minx2

70%ETOH 15minx2 70% 5minx2

95%ETOH 15minx2 95% 5minx2

100%ETOH 30minx2 100% 10minx2

100%PO 15minx2 10minx2

3:1 PO:EPON 3-4hrs 30min

2:1 PO:EPON 12-16hrs 30min
(overnight w/ caps off)

1:1 PO:EPON 12-16hrs 1hr
(overnight w/ caps off) +1hr (w/
caps off)

100%EPON 8hrs in embedding mold 4hrs

(w/ caps off)

bake for 24-48 hours in 60oC oven DMP .2ml/10ml
resin


Note: EPON 812 (which was discontinued in 1979) can be substituted with
EMBED 812 (EMS), Polybed 812 (Polysciences), Eponate 12 or Medcast
(TedPella). All have the ingredients DDSA, NMA, and DMP-30,
plus one 'company specific ingredient'.

I pasted this in from Word, I hope it makes some sense.

Bob Underwood
Morphology Core
Univ. of Washington
-

On Sun, 7 Sep 1997, maria lucia ribeiro caldas wrote:

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} I am having technical problems in EM skin biopsies. I would like to have
} a detailed protocol for processing skin biopsies.
}





From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Tue, 9 Sep 1997 13:09:20 -0400
Subject: RE: Chipped bell jars

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Your question is very broad so you will have to narrow the following list to
your interests. Enjoy the search!:

Bibliography

Aherne, W.A. and M.S. Dunnill 1982 Morphometry. Edward Arnold (Publishers)
Ltd. London,-205.

Bertram, J.F. and R.P. Bolender 1990 Counting cells with stereology: Random
versus serial sectioning. J Electron Microsc Techn, 14:32-38.

Bianciardi, G., P. Tanganelli, and G. Weber 1993 Blood cell activation: New
perspectives from ultrastructural morphometry. Semin Thromb Hemost,
19:108-114.

Bolender, R.P. 1978 Correlation of morphometry and stereology with
biochemical analysis of cell fractions. Int Rev Cytol, 55:247-289.

Bolender, R.P. 1981 Stereology: applications to pharmacology. Annu Rev
Pharmacol Toxicol, 21:549-573.

Bolender, R.P. 1982 Stereology and its uses in cell biology. Ann NY Acad
Sci, 383:1-16.

Bolender, R.P. 1992a Quantitative morphology for biologists and computer
scientists: I. Computer-aided tutorial for biological stereology (version
1.0). Microsc Res Techn, 21:338-346.

Bolender, R.P. 1992b Biological stereology: history, present state, future
directions. Microsc Res Techn, 21:255-261.

Cruz-Orive, L.-M. 1980 On the estimation of particle number. J Microsc,
120:15-27.

Cruz-Orive, L.-M. and E.R. Weibel 1981 Sampling designs for stereology. J
Microsc, 122:235-257.

Cruz-Orive, L.-M. 1987 Particle number can be estimated using a disector of
unknown thickness: the selector. J Microsc, 145:121-142.

Cruz-Orive, L.-M. and E.R. Weibel 1990 Recent stereological methods for cell

biology: a brief survey. Am J Physiol, 258:L148-56.

Dardick, I. and D. Caldwell 1985 Reproducibility of morphometric image
analysis. Hum Pathol, 16:1178

Freedman, L.S. 1974 A note on Aherne's method of counting tissue components
in relatively thick sections. J Microsc, 100:219-225.

Gundersen, H.J.G. 1977 Notes on the estimation of the numerical density of
arbitrary profiles: the edge effect. J Microsc, 111:219-223.

Gundersen, H.J.G., M. Boysen, and A. Reith 1981 Comparison of semiautomatic
digitizer-tablet and simple point counting performance in morphometry.
Virchows Arch B Cell Pathol, 37:317-325.

Gundersen, H.J.G. 1986 Stereology of arbitrary particles. A review of
unbiased number and size estimators and the presentation of some new ones,
in memory of William R. Thompson. J Microsc, 143:3-45.

Gundersen, H.J.G. and E.B. Jensen 1987 The efficiency of systematic sampling


in stereology and its prediction. J Microsc, 147(3):229-263.

Gundersen, H.J.G. 1988 The nucleator. J Microsc, 151:3-21.

Hammel, I., D. Lagunoff, M. Bauza, and E. Chi 1983 Periodic, multimodal
distribution of granule volumes in mast cells. Cell Tissue Res, 228:51-59.

Henderson, W.R. and E.Y. Chi 1985 Ultrastructural characterization and
morphometric analysis of human eosinophil degranulation. J Cell Sci,
73:33-48.

Loud, A.V., W.C. Barany, and B.A. Pack 1965 Quantitative evaluation of
cytoplasmic structures in electron micrographs. Lab Invest, 14:258-270.

Loud, A.V. and P. Anversa 1984 Biology of disease: Morphometric analysis of
biologic processes. Lab Invest, 50:250-261.

Loud, A.V. 1987 Electron microscopic morphometry. Anal Quant Cytol Histol,
9:7-12.

Maser, M.D. 1990 An overview of morphometry and stereology. Tx A&M Univ EM
Views, Issue No. 4:3-8,15-16.

Mathieu, O., L.-M. Cruz-Orive, H. Hoppeler, and E.R. Weibel 1981 Measuring
error and sampling variation in stereology: comparison of the efficiency of
various methods for planar image analysis. J Microsc, 121:75-88.

Mayhew, T.M. and M.A. Williams 1971 A comparison of two sampling procedures
for stereological analysis of cell pellets. J Microsc, 94:195-204.

Mayhew, T.M. and L.-M. Cruz 1973 Stereological correction procedures for
estimating true volume proportions from biased samples. J Microsc,
99:287-299.

Mayhew, T.M. and L.-M. Cruz-Orive 1974 Caveat on the use of the Delesse
principle of areal analysis for estimating component volume densities. J
Microsc, 102:195-207.

Mayhew, T.M. 1979 Basic stereological relationships for quantitative
microscopical anatomy - a simple systematic approach. J Anat, 129:95-105.

Mayhew, T.M. and F.H. White 1980 Ultrastructural morphometry of isolated
cells: methods, models and applications. Pathol Res Pract, 166:239-259.

Mayhew, T.M. 1981 On the relative efficiencies of alternative ratio
estimators for morphometric analysis of cell membrane surface features. J
Microsc, 122:7-14.

Nazeran, H., F. Rice, W. Moran, and J. Skinner 1995 Biomedical image
processing in pathology: a review. Australas Phys Eng Sci Med, 18:26-38.

Peachey, L.D. 1982 A simple digital morphometry system for electron
microscopy. Ultramicrosc, 8:253-262.

Pesce, C.M. 1985 Morphometric studies need review by statisticians. Am J
Clin Pathol, 83:258

Pesce, C.M. 1987 Biology of disease. Defining and interpreting diseases
through morphometry. Lab Invest, 56:568-575.

Pitha, J.V. 1985 Computer-assisted planimetry. Hum Pathol, 16:1284-1285.

Schmid-Sch”nbein, G.W. and S. Chien 1989 Morphometry of human leukocyte
granules. Biorheol, 26:331-343.

Sokal, R.R. and F.J. Rohlf 1981 Biometry: the principles and practice of
statistics in biological research. Freeman, San Francisco,

Sokol, R.J., G. Hudson, J. Wales, and N.T. James 1988 Morphometry of human
blood leukocyte ultrastructure: Its potential value in haematology.
Haematol, 21:129-139.

Sokol, R.J., G. Hudson, J. Wales, and N.T. James 1991 Ultrastructural
morphometry of human leucocytes in health and disease. Electron Microsc Rev,
4:179-195.

Sterio, D.C. 1986 The unbiased estimation of number and sizes of arbitary
particles using the disector. J Microsc, 134:127-136.

Stoeber, W. 1965 Statistical size distribution analysis. Lab Invest,
14:892-908.

Underwood, E.E. 1970 Quantitative stereology. Addison-Wesley, Reading,

Vedel Jensen, E.B. and H.J.G. Gundersen 1992 The rotator. J Microsc,
170:35-44.

Webster, P. and G. Griffiths 1994 A novel method for mean cell volume
estimation. J Microsc, 174:85-92.

Weibel, E.R., G.S. Kistler, and W.F. Scherle 1966 Practical stereological
methods for morphometric cytology. J Cell Biol, 30:23-38.

Weibel, E.R. 1969 Stereological principles for morphometry in electron
microscopic cytology. Int Rev Cytol, 26:235-302.

Weibel, E.R. 1972 The value of stereology in analysing structure and
function of cells and organs. J Microsc, 95:3-13.

Weibel, E.R. and R.P. Bolender 1973 Stereological techniques for electron
microscopic morphometry. In: Principles and techniques of electron
microscopy: Biological applications. Vol. 3. M.A. Hayat, ed. Van Nostrand
Reinhold Co. New York, pp. 237-296.

Weibel, E.R. 1974 Selection of the best method in stereology. J Microsc,
100:261-269.

Weibel, E.R. 1975 Quantitation in morphology: possibilities and limits.
Beitr Pathol, 155:1-17.

Weibel, E.R. 1981 Stereological methods in cell biology: where are we--where
are we going? J Histochem Cytochem, 29:1043-1052.

Weibel, E.R. 1982 Biomorphometry in physiological and pathological research.
Acta Med Pol, 23:115-125.

Weibel, E.R. 1989 Measuring through the microscope: development and
evolution of stereological methods. J Microsc, 155:393-403.

Weibel, E.R. 1991 Fractal geometry: a design principle for living organisms.
Am J Physiol, 261:L361-9.
-------------------------------------
Name: Charles Gilbert VOC:(704)355-5261
Carolinas Medical Center FAX:(704)355-8424
Dept of Pediatric Research
PO Box 32861
Charlotte, NC 28232-2861


Someone recently commented about having a glassblower grind down a bell jar
that has a chipped rim. We have had this done a number of times with good
success. It is indeed a feasible solution, provided you can find a
glassblower willing to undertake the task. On the other hand, you could
probably do it yourself, if you are willing to devote the time and energy
required, because all one glassblower I observed did was to spread a slurry
of silicon carbide abrasive on a piece of window glass, and sit and rub the
bell jar around over it.

Alternatively, we have been successful in some instances in 'patching' the
chips in bell jars using a stiff epoxy (e.g. Torr Seal). Clean all grease
and oil off the bell jar (try using Tilex Soap Scum Remover), then fill the
chipped hole with the epoxy, and set the bell jar on a flat, smooth surface
covered with waxed paper (so that the surface comes out flat and smooth)
while the epoxy cures.
Good luck,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:313-763-4788; Ph:313-764-3321






From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 9 Sep 1997 11:42:33 -0600 (MDT)
Subject: Re: TEM skin biopsy problems

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Hi,

Please define your problems more clearly. I have a lot of experience
with skin biopsies, (2 published papers also) but I need to know what the
problems are - embedding,
cutting, staining for LM, wrinkles, etc. I would be happy to help if I
had the details. Also, the size of the biopsy which you are required to
handle will have a great influence on your final protocol. My handout at
FORUM booth at the MSA meeting was an exact protocol which we use in our
laboratory. I would be happy to send it to you, if I knew it would be of
use in solving your particular problem. (Need your address).

Bye,
Hildy




From: shAf :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 09 Sep 1997 12:07:35 -0700
Subject: Re: Chipped bell jars

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Wil Bigelow wrote:

} ...
} Someone recently commented about having a glassblower grind down a bell jar
} that has a chipped rim. We have had this done a number of times with good
} success. ...

I consider a bell jar with any defect to be a considerable risk for an
implosion.If your users don't use a implosion gaurd with 100% consistency then
replace the
bell jar ...


cheerios, shAf
--
{\/} /\ {\/} /\ {\/} /\ {\/} cogito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/






From: shAf :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 09 Sep 1997 12:07:35 -0700
Subject: Re: Chipped bell jars

Contents Retrieved from Microscopy Listserver Archives
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Wil Bigelow wrote:

} ...
} Someone recently commented about having a glassblower grind down a bell jar
} that has a chipped rim. We have had this done a number of times with good
} success. ...

I consider a bell jar with any defect to be a considerable risk for an
implosion.If your users don't use a implosion gaurd with 100% consistency then
replace the
bell jar ...


cheerios, shAf
--
{\/} /\ {\/} /\ {\/} /\ {\/} cogito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/






From: Nancy A. Monteiro-Riviere, Ph.D. :      Nancy_Monteiro-at-ncsu.edu
Date: Tue, 09 Sep 97 14:13:08 -0500
Subject: skin biopsies

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-- [ From: Nancy A. Monteiro-Riviere, Ph.D. * EMC.Ver #3.1a ] --


Maria,
We have worked with skin from a variety of species for over 20 years. We
primarily work with human, pig, and in vitro equivalents. We do SEM, TEM,
Immuno EM, enzyme histochemistry and basic light microscopy. We will be
happy to share any of our techniques with you. What type of problem are you
having? We use half strength Karnovsky's for fixation and embed in Spurr.
Along time ago we used EPON 812 and then switched to Polybed and now Spurr.
Sectioning with a diamond knife greatly enhances your sections.Please let us
know your specific problems. Good Luck!!!
NAMR

Nancy A. Monteiro-Riviere,Ph.D.,DABFE,DABFM
Professor of Investigative Dermatology/Toxicology
North Carolina State University
College of Veterinary Medicine
Cutaneous Pharmacology and Toxicology Center
4700 Hillsborough St.
Raleigh, NC 27606
Telephone:(919)829-4426
FAX:(919)829-4358
email: Nancy_Monteiro-at-ncsu.edu
CTPC Homepage:http://cptc.ncsu.edu




From: admin-at-scottscientific.com (Scott Scientific Inc.)
Date: Tue, 9 Sep 1997 17:20:27 -0400 (EDT)
Subject: skin biopsies

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We are interested in getting a list/address of the different scientific
newsgroups/listservers on the internet.

Thank you all in advance,

Marla

Please send your response to ms-at-scottscientific.com





From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 9 Sep 1997 16:36:23 -0600
Subject: RE: Chipped bell jars

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Allow me to second this repair method. I used it to repair a seriously
chipped Denton 502A bell jar (it would work on any), and after overnight
curing (room temperature), the unit pulled as good a vacuum as quickly as
it did before the chip.

Phil

} Alternatively, we have been successful in some instances in 'patching' the
} chips in bell jars using a stiff epoxy (e.g. Torr Seal). Clean all grease
} and oil off the bell jar (try using Tilex Soap Scum Remover), then fill the
} chipped hole with the epoxy, and set the bell jar on a flat, smooth surface
} covered with waxed paper (so that the surface comes out flat and smooth)
} while the epoxy cures.
} Good luck,
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:313-763-4788; Ph:313-764-3321

}}}}}}}}}}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{{{{{{{{{{

Philip Oshel
Station A
PO Box 5037
Champaign, IL 61825-5037
(217) 355-1143
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again *****







From: :      yoyodine-at-UNM.EDU
Date: Tue, 9 Sep 1997 16:49:34 -0600 (MDT)
Subject: RE: Chipped bell jars

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 9 Sep 1997, Wil Bigelow wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Someone recently commented about having a glassblower grind down a bell jar
} that has a chipped rim. We have had this done a number of times with good
} success. It is indeed a feasible solution, provided you can find a
} glassblower willing to undertake the task. On the other hand, you could
} probably do it yourself, if you are willing to devote the time and energy
} required, because all one glassblower I observed did was to spread a slurry
} of silicon carbide abrasive on a piece of window glass, and sit and rub the
} bell jar around over it.
}
} Alternatively, we have been successful in some instances in 'patching' the
} chips in bell jars using a stiff epoxy (e.g. Torr Seal). Clean all grease
} and oil off the bell jar (try using Tilex Soap Scum Remover), then fill the
} chipped hole with the epoxy, and set the bell jar on a flat, smooth surface
} covered with waxed paper (so that the surface comes out flat and smooth)
} while the epoxy cures.
} Good luck,
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:313-763-4788; Ph:313-764-3321
}
}
We have repaired our Bell Jars 10-15 times using Vacuum epoxy, such as
Bell Torr or Torr Seal. We do it a bit different than above. We actually
file and sand the surface smooth. Basically, I have come to the
conclusion that for the level of vacuum that Denton Coaters use, the only
time a new bell needs to be bought is if it cracks. Chipping is easy to
take care of.

oh...The reason I replied to the above is that if you use the wax paper
technique, make sure you get the epoxy thick enough in the chip (ie. as
thick as the glass)

Christopher Adcock






From: schmutzm-at-lear.u-strasbg.fr (Schmutz Marc)
Date: Wed, 10 Sep 1997 10:09:42 +0900
Subject: RE: Chipped bell jars

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Hi microscopy netters around the world,



In behalf of the "boss" of the EM core, I'll ask you for help...
We like to know if there is somebody around who used LR white resins in an
AFS from Leica. What are your conditoins, problems encountered etc...
Thanks a lot for your help



Marc




PS Thank you Nestor for your work...










------------------------------
SCHMUTZ Marc PhD
IGBMC
1 rue Laurent FRIES
BP 163
F 67404 Illkirch Cedex
FRANCE

Tel: +33 (0)388 653 330 direct
Fax: +33 (0)388 653 201
email:schmutzm-at-lear.u-strasbg.fr

------------------------------






From: Angus Bewick :      phab-at-siva.bris.ac.uk
Date: Wed, 10 Sep 1997 15:29:55 BST
Subject: SEM-beam deflection with old Coates&Welter

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Dear all

I am a user of an old Coates&Welter/Nanometrics FEG-SEM and am interested in
producing Electron Channeling Patterns. I need to rock the beam about a point
on the sample for this but my microscope doesn't have this capability.

Are there any users (probably ex-users!) of this venerable machine who can
tell me whether a beam deflection system was ever produced for the C&W or who
have experience of ECPs with it? If so, where can i get hold of the necessary
electronics?

Any help would be much appreciated.


ANGUS BEWICK
Physics Dept.
University of Bristol
UK
phab-at-siva.bris.ac.uk




From: Patrick Huddie :      phuddie-at-microcosm.com
Date: Wed, 10 Sep 1997 10:40:09 -0400
Subject: Re: optical microscopy question

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This is a multi-part message in MIME format.
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Barbara Foster wrote:

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} America
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} ListServer-at-MSA.Microscopy.Com
} -----------------------------
} -----------------------------------------.
} Born & Wolf's book on the Physics of Optics is also a good reference.
} I have no info on publisher, etc.
} Let me know what you find.
}
} Best regards,
} Barbara Foster
} Microscopy/Microscopy Education
} 53 Eton Street
} Springfield, MA 01108
} PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com

Barbara:

I think you may mean:

Principles of Optics : Electromagnetic Theory of Propagation,
Interference and Diffraction of Light
by M. Born, E. Wolf
Sixth Edition (Paperback) Published by Pergamon Press
Publication date: June 1981 ISBN: 0080264816


--------------------------------------------------------------
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Microcosm, Inc., 9140 Guilford Road, Suite O, Columbia, MD 21046
e-mail phuddie-at-microcosm.com URL http://www.microcosm.com
The Web is:"Vaster than empires and more slow" Andrew Marvell
(1621-1678)


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From: valdemar :      valdemar-at-fast.net
Date: Wed, 10 Sep 1997 10:47:56 -0400
Subject: SEM - where to sell used SEM + EDS & for how much?

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We are replacing our old SEM + EDS and would like dispose of it ourselves
since the manufacturer of the new unit is offering only a nominal sum in a
trade in.
Can anyone suggest effective places to advertise this equipment?
(We are located in NE USA, and would like to make room for the new system
in 3-4 mo.)

A follow up question that I have is: what is a reasonable price to ask?
( The SEM is a 16 y.o. AMRAY 1600 turbo LaB6/W, secondary & Link
backscatter,
2 CRT's, vibration isolation table, in good condition & under factory
service contract.
The EDS is 3 y.o. Oxford Isis thin window 136eV spec., 126eV MnKa
calibrated,
with beam control and image capture, under factory service contract. )

I will greatly appreciate any & all suggestions | comments.

Valdemar Furdanowicz
valdemar-at-fast.net





From: T. Graham :      shiva-at-u.washington.edu
Date: Wed, 10 Sep 1997 09:48:54 -0700 (PDT)
Subject: Chromium

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I need to get ahold of a chromium sputtering target. Does anyone know
which company I may purchase this from?

Tom Graham






From: Barbara Foster :      mme-at-map.com
Date: Wed, 10 Sep 1997 12:51:17 -0700
Subject: RE: Infinity... "Little Book"

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Dear colleagues,

Many thanks for your numerous and energetic inquiries about "Optimizing
Light Microscopy for Biological and Clinical Labs"! Since our web site
is not quite ready, in answer to those requests, we have put together a
short order form (see below) as well as the description sent in an
earlier email "re: infinity optics".

For those of you in the Northeast, you can also get a complimentary copy
of the book by attending the one-day lecture-demo, "Optimizing Light
Microscopy". Dates: Oct 3 (Providence College, Providence RI),
Nov 3 (NYC), Nov 5 (Springfield, MA), Nov 7 (Boston, Tufts Med School
MRC) Email for details.

Book Description:
"Optimizing Light Microscopy for Biological and Clinical Laboratories" is
available from MME as well as through the American Society for Clinical
Laboratory Sciences and the publisher, Kendall-Hunt. Approximately 200
pages with over 100 diagrams, illustrations, and micrographs. Peppered
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Many thanks for your order. We hope you enjoy "Optimizing Light
Microscopy". Let us hear from you with successes and/or comments.

Barbara Foster
Consortium President
MME
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-Microscopy/Microscopy Education is a consortium of 24 consultants who
specialize in customized on-site training in all areas of microscopy,
sample preparation, and image analysis. Our goal: to help you use your
microscope more effectively.
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From: Craig Lending :      clending-at-acs.brockport.edu
Date: Wed, 10 Sep 1997 16:13:13 -0400
Subject: Darkroom Doors and Stainless Steel Sink Suppliers?

Contents Retrieved from Microscopy Listserver Archives
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We are in the process of rebuilding our darkroom from the ground up, and
were wondering if anyone had any information on suppliers of the revolving,
light-tight darkroom doors and/or other darkroom fixtures.

Thanks.

Craig Lending
Department of Biology
SUNY Brockport
Brockport, NY 14420

Voice: 716-395-5755
Fax: 716-395-2741
e-mail: clending-at-acs.brockport.edu




From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 10 Sep 1997 16:53:42 -0400
Subject: Where to get Torr Seal

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Torr Seasl is an epoxy compound especially formulated for use in vacuum
systems that was sintroduced by Varian Associates, Vacuum Products
Division, 121 Hartwell Ave, Lexington, MA 02173-3133 (800-882-7426) a
number of years ago. It is stated to be good at pressures below 10-9Torr,
and is bakeable at temperarures up to 120C. It adheres to most clean
materials (glass, metals, ceramics) and holds up well over long term
service. Some companies that handle EM supplies also handle it (e.g. I
find it listed in the Ladd catalog).

Other similar products are also sold by other companies. For example,
Duniway Stockroom Corp. (800-446-8811; info-at-duniway.com) sells a product
called 'Epoxy Patch' that is stated to be equivalent to Torr seal

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:313-763-4788; Ph:313-764-3321






From: Edwards, Danny J :      dan.edwards-at-pnl.gov
Date: Wed, 10 Sep 1997 14:00:21 -0700
Subject: Unsubscribe

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-------------------------------------------------------
Dan Edwards
Structural Materials Research Section
Battelle Pacific Northwest National Laboratory
P.O. Box 999, MSIN P8-15
Richland, WA 99352

Office: 509-376-4867
Fax: 509-376-0418
E-mail: dan.edwards-at-pnl.gov






From: rw9-at-psu.edu (Rosemary Walsh)
Date: Wed, 10 Sep 1997 16:59:18 -0500
Subject: Problem-fixing imaginal discs (Drosophila)

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Hello,
We have a problem fixing imaginal discs
(third instar - Drosophila). We are following
a protocol used by Andrew Tomlinson, 1985-
"The cellular dynamics of pattern formation
in the eye of Drosophila" in J. Embryol. exp.
Morph. 89, 313-331. The fixative used
is a combined cold glutaraldehyde/osmium
followed by an osmium post-fix.
We're seeing pore fixation of membranes
including inner cristae of mitochondria and
some clear areas within cytoplasm.
Thanks in advance for any suggestions.
Rosemary

####################################################
Rosemary Walsh
Electron Microscope Facility for the Life Sciences
The Biotechnology Institute for Research and Education
1 South Frear Lab
University Park, PA 16802
814-865-0212 email:rw9-at-psu.edu
####################################################






From: Emitech-at-ix.netcom.com
Date: Wed, 10 Sep 1997 16:10:53 -0500 (CDT)
Subject: Chromium Targets

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We supply Chromium Targets. If you would like to give us a call at 800/444-3137 and
let us know what type of system you have, we will be happy to help you.

Linda S. Dailey
Emitech USA, Inc.





From: kennedy-at-nsi.edu (Grace Kennedy)
Date: Wed, 10 Sep 1997 15:52:51 -0800
Subject: osmium pepper

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At least once over the history of this listserver there has been extensive
discussion about osmium "pepper" and other artifactual inclusions seen in
embedded, sectioned TEM samples. Does anyone have a compilation of
replies/discussion or remember what conclusions were drawn? Answer
directly please, to avoid boring others with a rediscussion, OK? Many
thanks. Grace P.S. Was it related especially to phosphate buffered
osmium solutions?? I just had a major disaster with some and would like to
solve the problem quickly.....






From: Darrell Miles :      milesd-at-US.ibm.com
Date: Wed, 10 Sep 1997 18:56:27 -0400
Subject: Re: request for help

Contents Retrieved from Microscopy Listserver Archives
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The semiconductor industry uses "wafer" tweezers. They
are used to pick up wafers from the side. The contact
is not as minimal as the triceps, but they may work.
There are some listed at the following URL. I am not
sure where the ones we use around here came from.
URL: http://www.ebsciences.com/labsupply/tweezers.htm

Darrell




From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Wed, 10 Sep 1997 16:15:43 -0700 (PDT)
Subject: Pulling Protoplasts

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Oh Great Body of Knowledge,


We have a student in our lab who is having a problem with her
protoplasts that have been embedded in Epon/Araldite pulling away from the
resin when she sections. This causes a lot of problems, to say the least.
Do any of you have suggestions as to how to keep this from
happening? She has heard that adding tannic acid to the fix helps prevent
the problem. Has anyone done that?
She's also having a problem with the inclusion bodies falling out
of her sections when she cuts. Any suggestions about that? We recommended
she reduce her cutting speed.
All suggestions will be gratefully passed along to her.

Always eager to learn new things,


Paula = )

Paula Sicurello
UC Berkeley
Electron Microscope Lab
psic-at-uclink4.berkeley.edu






From: Nick Brecha :      nbrecha-at-ucla.edu
Date: Thu, 11 Sep 1997 02:20:30 -0700
Subject: Unsubscribe

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From: jss :      jss-at-siva.bris.ac.uk
Date: Thu, 11 Sep 1997 11:31:11 +0000
Subject: Subscribe

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To
The Sparc5. Microscopy Station Master

I have sent a repeated requests to resubscribe but without any results.
I should be most grateful if you can put me on the list. If there are
any problems, Please let me know.

Yours sincerely,


Jitu Shah




From: Microls-at-aol.com
Date: Thu, 11 Sep 1997 07:10:22 -0500
Subject: IM Analysis Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can anyone recomend a good, user friendly LM IM analysis software program?
Our current one is useless (hate to say). We will be demoing a Noesis
pacckage soon. I have experience with Optimas, and personally do not care
for it. Any other shareware besides NIH? All comments etc welcome.

Sincerely:
Lou Solebello
JM Huber Corp.






From: rgarcia-at-nova.wright.edu
Date: Thu, 11 Sep 1997 09:20:01 -0500 (EST)
Subject: Re: Darkroom Doors and Stainless Steel Sink Suppliers?

Contents Retrieved from Microscopy Listserver Archives
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Craig,

Bernie's Photo supply in Pittsburg is gret. They usualy have
everything you need. I will send along the phone number and address later
if you want.




From: Jim Darley :      jim-at-proscitech.com.au
Date: Thu, 11 Sep 1997 23:30:38 +1000
Subject: EDS quantitative anaylisis/ detection limit

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I posted the below earlier this week but the email bounced back. I believe
that the the previously advanced concept of 'quantitative' analysis does
require examination.
JD.

I thank Garratt-Reed for corroberating my previous posting. John Bazzola
had asked for a rough guide (he has confirmed that since) on detection
limits of EDS versus WDS techniques. Anybody who has any significant
experience with microprobe analysis knows that there is no single line
correct answer. However, it is imperative for analysts to remember a few
general figures so they can advise on appropriate instrumentation and
techniques.

John B's initial inquiry deserved a reply and when none was given, I
posted mine more than a day later. I believe that my posting is a useful
guide
for non-specialist analysts. Nothing that GR writes makes nonsence of my
posting.

Nobody had asked about other differences between the techniques eg.
resolution or simultaneous acquisition. I am pleased that G-R has supplied
some information on those topics and on new, very high count-rate
acquisition facilities for EDS. G-R is proud of his 0.6% "quantitative
analysis" of Cr at an accuracy of +/- 0.1wt%.

I suggested that in EDS the presence of 1% of an element is the
approxiamte lower limit for quantitative
analysis. G-R has lowered that limit by some 40% - or has he? +/- 0.1% is
good when 20% of the element is present, as it represents an accuracy of
0.5%. +/- 0.1% when 0.6% is present is about +/- 16% accuracy; I call
that qualitative or at best semi-quantitative.

Another correspondent emailed me and noted that it was President Trueman
who had been looking for a one handed adviser. But that was for an
economist and not a scientist as I, apparently wrongly, remembered. The
correspondent
could see my point though; thanks to Brian Demczyk. I think that Trueman
had an
excellent idea but he should have extended that search to a scientist as
well. Certainly microscopists and economists share disciplines which
combine art and science. And I should add require 'good judgement'.

Why now was I abused with that opening: "Jim Darley's posting is hardly
furthering good science". Am I to believe that good science is advanced
by quarelsome nitpicking and that all broadbanding is verboten? I plead
not guilty.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au

} } Subject: Re: detection limits x-ray
} } Date: Friday, 5 September 1997 6:21
} }
} } Jim Darley's posting is hardly furthering good science.
} }
} } 1: Detection limits (John's original question). Modern EDX systems
are
} } easily capable of quantitative analysis in the sub-1wt% range. See,
for
} } example http://prism.mit.edu/facltis/stem/stmexam.htm (the second
} } illustration on that page) where I was getting analyses for Cr in steel
} of
} } the order of 0.6+/-0.1 wt%. I don't know about you, but I consider
this
} } quantitative. This, mind you, was in a measurement where I was
} attempting
} } to optimize spatial resolution rather than sensitivity, and the
} acquisition
} } time was 60sec. per data point. This example, of course, was from a
} STEM.
} } There are many more examples in the literature.
} }
} } Using beam gating techniques such as those developed by Charlie Lyman
and
} } colleagues, EDX data can readily be acquired at 20,000-40,000 counts
per
} } real second, if spatial resolution is sacrificed. Combine this with an
} } acquisition time of 1,000 seconds (by no means unrealistic for an
} important
} } measurement) and the detection limit is in the rage of, or better than,
} 0.01wt%.
} }
} } Of course, in the SEM, which may have been the point of John's original
} } question, the situation is not the same, the beam voltage is lower
} } (resulting in poorer peak/bremmstrahlung ratios) and the emission of
} x-rays
} } from a solid sample is different from that in a thin foil, but these
are
} } some of the variables that Michael quite rightly pointed out must be
} considered.
} }
} } 2: Comparison between EDX and WDX.
} }
} } This is like comparing apples and oranges, because the instruments
} designed
} } with them are generally intended for different purposes.
} }
} } WDX has a much better peak resolution than WDX, which results in better
} } measured P/B ratios (and hence improved statistics), as well as much
} better
} } capability in resolving nearby x-ray lines. Also, because the x-ray
} } counting and wavelength analysis are different functions in the crystal
} } spectrometer, available countrates have traditionally been higher in
WDX
} } than EDX (although modern EDX detectors are an order of magnitude
faster
} } than they were fifteen years ago). Against this must be set the fact
} that
} } WDX is inherently a serial technique (although multiple spectrometers
} help
} } here), while EDX is a parallel technique (compare the advantages of
PEELS
} } over SEELS - although this is not a totally fair comparison). Anyway,
} the
} } advantage of WDX (ignoring the capability of resolving peak overlaps)
is
} } improved statistical precision. Is this useful?
} }
} } Well, maybe.
} }
} } By far the most significant parameter which affects electron-induced
} x-ray
} } emission spectra is sample geometry. Two extreme cases are where the
} sample
} } is a thin foil (as in the STEM) where, to a first approximation, the
} x-ray
} } spectrum recorded by the detector is the same as that emitted, and to a
} } second order, it is possible to derive a reasonable thickness
correction
} for
} } cases where the error is small. Alternatively, when the sample has
} } dimensions large compared with the volume irradiated by the electron
} beam,
} } and has an accurately known geometry compared to the incident beam and
} the
} } detector (such as a polished flat sample in the microprobe), correction
} } programs such as ZAF can do a reasonable job of extracting a
quantitative
} } analysis.
} }
} } What about where the sample geometry is unknown (for example, a rough
} } surface such as might be examined in the SEM)? In that case the
} uncertainty
} } in the analysis is far, far worse than any uncertainty caused by the
poor
} } statistics of the EDX spectrum, so there is no point or advantage
} whatever
} } in using WDX to try to improve things, because it won't. This is why
} } typically an SEM has an EDX detector - it is cheaper and gives just as
} good
} } an analysis (except for the somewhat poorer detection limit). In the
} } microprobe, great care is taken in polishing and mounting the sample,
so
} the
} } advantage of the WDX detector can be realised.
} }
} } Where does this leave us? The advantage of a WDX detector over an
EDX
} } detector *ON THE SAME SAMPLE* is limited - perhaps an order of
magnitude
} in
} } detection limit, and, on a flat, polished sample, also perhaps
} approaching
} } an order of magnitude in precision. The WDX detector can also resolve
} many
} } cases where peaks would overlap in EDX. On general rough SEM samples,
} the
} } only advantages of WDX are a small improvement in detection limits and
} the
} } ability to resolve overlaps (which could be important if a trace
element
} } peak overlaps a major peak in the EXD spectrum.
} }
} } The President's science advisors were right - it all depends. I seem
to
} } remember that one of Jim Darley's Prime Ministers (Gough Whitlam?) had
to
} be
} } dismissed because Parliament would not pass his budget - but does that
} have
} } anything to do with Science?
} }
} } Tony Garratt-Reed
} }
} }
} } } Hi John and Michael et al:
} } } I recall that one of your Presidents (Kennedy?) was looking for a one
} } } handed science adviser; the ones he had always prevaricated "on the
one
} } } hand and on the other hand".
} } }
} } } Michael is quite right, detection limits vary greatly depending on
} endless
} } } factors. However, it is useful to have some figures as guidelines:
} } } Considering say the 10 elements following sodium. Detection of these
is
} } } about best.
} } } For these in EDX the limit for quantitative analysis is about 1%.
} Detection
} } } limit is about 0.1%. Increasing counts and counting times beyond the
} } } customary 100 seconds at perhaps 2000cps will scarcely improve either
} } } limit.
} } }
} } } WDX is near quantitative to its detection limit and that is at least
two
} } } orders of magnitude greater than is EDX.
} } }
} } } I'll enter correspondence only when it concerns errors in excess of
five
} } } orders of magnitude.
} } } Cheers
} } } Jim Darley
} } }
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Phone +61 77 740 370 Fax: +61 77 892 313
} } } Great microscopy catalogue, 400+ Links, MSDS
} } } ************************ http://www.proscitech.com.au
} } } } John J. Bozzola wrote:
} } } } }
} } } } } Under ideal (and reasonably attainable conditions), what is the
} } } } } detection
} } } } } limit (in grams) for EDX and WDX? Thanks.
} } } }
} } } } The question you ask is not specific enough ... to many factors to
} be
} } } } considered with regard to which elements you are interested in, and
} } } } (e.g.) ... how sensitive your specimen is to long count times and
high
} } } } beam currents ... ... ask again ...
} } } } cheerios, shAf
} } Anthony J. Garratt-Reed
} } MIT Room 13-1027
} } 77 Massachusetts Avenue
} } Cambridge, MA 02139-4307
} } United States of America
} }
} } Ph: 617-253-4622
} } Fax: 617-258-6478





From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 11 Sep 1997 10:17:44 -0400
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone else like to help this soul. Send mail to his address
(mluiselli-at-davinci.cnart.mx) not mine. Thanks






} Return-Path: {mluiselli-at-davinci.cnart.mx}
} Date: Wed, 10 Sep 1997 18:33:42 -0700
} From: "Lic. Mariana Luiselli" {mluiselli-at-davinci.cnart.mx}
} Organization: C N C A
} To: sdw-at-biotech.ufl.edu
} Subject: can you help me to find out what is K=F6eler lighting...
} X-URL: http://www.biotech.ufl.edu/~emcl/tips.html
}
} In my homework they ask me, what is k=F6eler lighting, but i don=B4t know=
=20
} what it is. Can you help me to find it out?
} Thank you so very much,
} mariana luiselli
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "









From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Thu, 11 Sep 1997 10:30:35 -0400 (EDT)
Subject: Thanks---CM12 Power Supply

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who responded to my CM12 +24 volt power supply problem.

The answer to the problem is to change C104 and C106, 47 ufd. 40 volts
capacitors to 47 ufd. 63 or 100 volts. These capacitors are in the
auxiliary power supply for startup, within the unit.

After the repair, I powered up the microscope and it worked the first
time.

Thanks again
Fred


********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1

********************************************************





From: Dekkete :      Dekkete-at-prince.sprint.com
Date: Thu, 11 Sep 1997 11:06:40 -0400
Subject: Information re. Sonic Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Can you refer me to educational information regarding the science,
application, and interpretation of sonic microscopy?




From: Mª DOLORES GOMEZ JIMENEZ :      gomezm-at-ibmcp.upv.es
Date: Thu, 11 Sep 1997 17:07:57 -0700 (PDT)
Subject: LM - Need help on staining protoplasts

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I=B4m interested in staining protoplast with DAPI, but I don=B4t know how d=
o=20
it.Is necessary a fixation?. I would like know a protocol to make this=20
staining.
Best regards,
M.D.Gomez
email: gomezm-at-plantas.ibmcp.upv.es
=20




From: EUGENE GORDON :      MEDJET-at-worldnet.att.net
Date: Thu, 11 Sep 1997 11:31:11 -0400
Subject: Re: osmium pepper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

If at all possible, could compilations of replies/discussion regarding
osmium pepper be posted on the list server. I know that would serve useful
for myself. Thanks.

Dan Caruso
Biological Technician
Medjet-at-worldnet.att.net

----------
} From: Grace Kennedy {kennedy-at-nsi.edu}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: osmium pepper
} Date: Wednesday, September 10, 1997 7:52 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} At least once over the history of this listserver there has been
extensive
} discussion about osmium "pepper" and other artifactual inclusions seen in
} embedded, sectioned TEM samples. Does anyone have a compilation of
} replies/discussion or remember what conclusions were drawn? Answer
} directly please, to avoid boring others with a rediscussion, OK? Many
} thanks. Grace P.S. Was it related especially to phosphate buffered
} osmium solutions?? I just had a major disaster with some and would like
to
} solve the problem quickly.....
}




From: DanCTSC-at-aol.com
Date: Thu, 11 Sep 1997 11:40:58 -0400 (EDT)
Subject: Subscribe

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Subscribe




From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Thu, 11 Sep 1997 12:01:48 -0400
Subject: Re: Darkroom Doors and Stainless Steel Sink Suppliers?

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At 04:13 PM 9/10/97 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Consolidated International Corp.
4501 South Western Blvd
Chicago, IL 60609
800/621-3680
312/376-5600
312/376-5835 FAX

I don't know if there are other suppliers. Be very careful with the
installation. We've had difficulties with ours primarily due to an
incompetent installation.

Henk




From: Ya Chen :      ychen14-at-facstaff.wisc.edu
Date: Thu, 11 Sep 1997 11:11:41 -0600
Subject: Chromium Target supplier

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

We got our chromium target from Technotrade, a US company which services
Balzers' equipment. Phone: 603-622-5011/ 1-800--875-3717.

I am not financially associated with that company.

Ya Chen


Ya Chen

=====================================================================
\ / Integrated Microscopy Resource (IMR)--
\ / __ an NIH Biomedical Research Resource TEL: 608-263-8481
\/ / / University of Wisconsin-Madison FAX: 608-265-4076
/ / / 1675 Observatory Dr. #159
/ /__/_ Madison, WI 53706 Email:ychen14-at-facstaff.wisc.edu
=====================================================================
IMR Home Page: http://www.bocklabs.wisc.edu/imr.html







From: Kalman Rubinson :      rubinsnk-at-is2.nyu.edu
Date: Thu, 11 Sep 1997 13:26:22 -0400 (EDT)
Subject: Re: IM Analysis Question

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On Thu, 11 Sep 1997 Microls-at-aol.com wrote:

} Can anyone recomend a good, user friendly LM IM analysis software program?
} Our current one is useless (hate to say).

Which one is it?

} We will be demoing a Noesis pacckage soon.

Superb but not particularly friendly. Steep learning curve.

} I have experience with Optimas, and personally do not care
} for it. Any other shareware besides NIH? All comments etc welcome.

ImagePro is probably the most friendly and is fairly powerful. For
shareware, I strongly suggest you look at ImageTools
(http://ddsdx.uthscsa.edu/dig/itdesc.html).
An alternative which I liked quite a bit less but which seems powerful is
Osiris
(http://www.expasy.ch/www/UIN/html1/projects/osiris/ReadmeOsiris.html).

Kal





From: malcolm.haswell-at-sunderland.ac.uk (HASWELL Malcolm)
Date: Thu, 11 Sep 1997 18:45:00 +0100
Subject: Storing Karnovsky Fixative

Contents Retrieved from Microscopy Listserver Archives
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I want to supply Karnovsky (modified to 2.5% G and 2.5% "paraformaldehyde")
to a lab to collect 5mm biopsies over a period of time.
In the past I have used Karnovsky for up to a week after producing it but I
am not sure about storing it for 1-2 months. Should I store at 4 deg C or
consider freezing some (it will be in caodylate with 2.5mM CaCl2)?

Malcolm Haswell
Electron Microscopy
University of Sunderland





From: dmrelion-at-world.std.com (donald j marshall)
Date: Thu, 11 Sep 1997 13:44:53 -0400
Subject: Trueman

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In a recent discussion I excerpted this:

"Another correspondent emailed me and noted that it was President Trueman
who had been looking for a one handed adviser. But that was for an
economist and not a scientist as I, apparently wrongly, remembered. The
correspondent
could see my point though; thanks to Brian Demczyk. I think that Trueman
had an"

I think this must have been a Freudian slip, and at certain times he might
have even been called President Bluntman, but his name was "Truman".

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 617-275-4695
FAX: 617-271-0252


email dmrelion-at-world.std.com





From: rschoonh-at-sph.unc.edu (Robert Schoonhoven)
Date: Thu, 11 Sep 1997 15:14:41 +0000 (est)
Subject: Re: IM Analysis Question-reply

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I've been using the BioQuant system for about 5 years now and love it. It's sold by R&M Biometrics
Inc. Phone 615-350-7866.

It's a Windows based program that is very versatile program, color recognition as well as grey
scale. It can do sterography, cell counts (I automated my cell proliferation studies with it), etc.


the usual discliamer :) I have no connection with this Co. except as a statisfied customer.
-- Begin original message --

} From: Microls-at-aol.com
}
} Can anyone recomend a good, user friendly LM IM analysis software program?
} Our current one is useless (hate to say). We will be demoing a Noesis
} pacckage soon. I have experience with Optimas, and personally do not care
} for it. Any other shareware besides NIH? All comments etc welcome.
}
} Sincerely:
} Lou Solebello
} JM Huber Corp.
}
}
}

-- End original message --


regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123





From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Thu, 11 Sep 1997 15:07:18 -0400
Subject: Thanks

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Hi, All,

I have just returned from holidays and have found a number of responses
related to the question of gelatinized glutaraldehyde I had posed just
before I left. Thanks to everyone for their interest. Oh by the way, I have
now been able to entice one of my chemist colleagues to look at some of
these samples to see whether she can discover a reason for this
phenomenon as a result of differences in berry chemistry. If anyone is
interested in the results, please contact me offline, but it will be awhile
before the chemical analyses are done.

Thanks again.

Paula.

Paula Allan-Wojtas
Food Microstructure Specialist
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia, Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca




From: GANTZ-at-med-biophd.bu.edu
Date: Thu, 11 Sep 1997 12:50:35 -0400 (EDT)
Subject: Darkroom doors and sinks

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Dear Craig:
Try Arkay Corporation, 228 South First Street, Milwaukee, Wisconsin
Phone #1-800-TO-ARKAY; 414-276-9196. My catalogue shows that they carry
both.
Don Gantz
Boston Univ. Med School




From: NANCY SMITH :      nsmith-at-gauss.sci.csuhayward.edu
Date: Thu, 11 Sep 1997 13:14:50 PSD8PDT
Subject: XL40 filament life

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I would be interested to hear from Philips XL30 & XL40 users
regarding how long their tungsten filaments last.

Thanks for your input.
Nancy R. Smith
Director of Operations
Microscope And Graphical Imaging Center
California State University, Hayward
http://www.csuhayward.edu/SCI/sem




From: John Arnott :      ladres-at-worldnet.att.net
Date: Thu, 11 Sep 1997 16:37:15 -0400
Subject: Re: Torr Seal /Bell Jars

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Wil Bigelow wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.}
} Torr Seasl is an epoxy compound especially formulated for use in vacuum
} systems that was sintroduced by Varian Associates, Vacuum Products
} Division, 121 Hartwell Ave, Lexington, MA 02173-3133 (800-882-7426) a
} number of years ago. It is stated to be good at pressures below 10-9Torr,
} and is bakeable at temperarures up to 120C. It adheres to most clean
} materials (glass, metals, ceramics) and holds up well over long term
} service. Some companies that handle EM supplies also handle it (e.g. I
} find it listed in the LADD catalog).
}
} Other similar products are also sold by other companies. For example,
} Duniway Stockroom Corp. (800-446-8811; info-at-duniway.com) sells a product
} called 'Epoxy Patch' that is stated to be equivalent to Torr seal
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:313-763-4788; Ph:313-764-3321

As a manufacturer of vacuum evaperators we have been plagued by chipping
of bell jars for many years.
We have used (and sold) Torr Seal and several other epoxies over the
years and they are satisfactory for small chips.
However, for large chips we have found that epoxy-putty GAPOX10(tm)
works extremely well. It is inexpensive and cures in one hour for
sanding and smoothing.
We can pull a vacuum of 10(-7) and have yet to encounter a problem.

Mike Bouchard
Vacuum Specailist
Ladd Research




From: kennedy-at-nsi.edu (Grace Kennedy)
Date: Thu, 11 Sep 1997 13:46:31 -0800
Subject: dichromate/osmium

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Thanks to all who responded to my osmium pepper question. I now know not
to osmicate in phosphate but haven't decided what I should use for a
buffer-I don't think phosphate and cacodylate are compatible so a simple
switch may be out. Has anyone out there used Dalton's dichromate/osmium? I
cannot find any reference to anything by Dalton. I do have a formula and
have used it but am curious to know if anyone else has played with it. Tx
Grace






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 11 Sep 1997 15:41:29 -0600 (MDT)
Subject: Re: dichromate/osmium

Contents Retrieved from Microscopy Listserver Archives
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} Thanks to all who responded to my osmium pepper question. I now know not
} to osmicate in phosphate but haven't decided what I should use for a
} buffer-I don't think phosphate and cacodylate are compatible so a simple
} switch may be out. Has anyone out there used Dalton's dichromate/osmium? I
} cannot find any reference to anything by Dalton. I do have a formula and
} have used it but am curious to know if anyone else has played with it. Tx
} Grace

In a former life, I used Dalton's fixative for immersion fixation of
vertebrate retina. I don't remember all the details, but the results were
very good. I don't remember this fix being widely used, though.

John
chandler-at-lamar.ColoState.EDU






From: Lucio Mulestagno :      luciom-at-NEWTON.UMSL.EDU
Date: Thu, 11 Sep 1997 17:17:16 -0500 (CDT)
Subject: IR microscopes for semiconductors

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Hi All,
does anyone know of a portable IR microscope vendor? A collleague of
mine would like to purchase one to be able to look at sub surface features
on silicon crystals as-grown. Since the crystals are rather large, it is
easier to take the microscope to the crystal then vice-versa if possible.

I appreciate any help.

cheers

Lucio



Dr.Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu





From: Patrick Huddie :      phuddie-at-microcosm.com
Date: Thu, 11 Sep 1997 18:19:35 -0400
Subject: Re: IM Analysis Question

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Microls-at-aol.com wrote:

} Can anyone recomend a good, user friendly LM IM analysis software
} program? Any other shareware besides NIH? All comments etc welcome.
}
} Sincerely:
} Lou Solebello
} JM Huber Corp.

ImageTool is a free image processing and analysis program for Windows
95/NT from the University of Texas Health Science Center at San Antonio.

http://ddsdx.uthscsa.edu/dig/itdesc.html

This list needs a FAQ.

--------------------------------------------------------------
Dr. Patrick L. Huddie (301) 725-2775 Fax (301) 725-2941
Microcosm, Inc., 9140 Guilford Road, Suite O, Columbia, MD 21046
e-mail phuddie-at-microcosm.com URL http://www.microcosm.com
The Web is:"Vaster than empires and more slow" Andrew Marvell
(1621-1678)






From: Rong Lijian :      ljrong-at-imr.ac.cn
Date: Fri, 12 Sep 1997 07:52:31 -0700
Subject: Re: Subscribe

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jss wrote:
}
} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}

} The Sparc5. Microscopy Station Master
}
unsubscribe





From: bozzola-at-siu.edu (John J. Bozzola)
Date: Thu, 11 Sep 1997 19:27:05 -0600
Subject: Re: dichromate/osmium

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} Thanks to all who responded to my osmium pepper question. I now know not
} to osmicate in phosphate but haven't decided what I should use for a
} buffer-I don't think phosphate and cacodylate are compatible so a simple
} switch may be out. Has anyone out there used Dalton's dichromate/osmium? I
} cannot find any reference to anything by Dalton. I do have a formula and
} have used it but am curious to know if anyone else has played with it. Tx


Grace,
In our experience, osmium pepper is not caused by phosphate problems but
by using aldehydes that have partially polymerized (e.g., old
glutaraldehyde or formaldehyde solutions). The polymers are small enough to
get into the cell but once attached to proteins, can not be removed. The
polymers then vigorously reduce osmium which then shows up as pepper. I
have routinely used phosphate buffered osmium and have never had the pepper
problem. In fact, however, for osmication you really don't need to buffer
at all. Distilled water is fine.

I used Dalton's chrome osmium many years ago for tissue culture cells and
it worked fine. Now we prefer to use osimum ferrocyanide which gives better
contrast. You can prep the solution using cacodylate. We fix in either
cacodylate OR phosphate buffered glut/form, rinse extensively (overnight)
in cacodylate buffer and then into the osmium.

If you need more info, contact me again as I can fax you the info for Dalton's.

John

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Neckers Building, Room 146 - B Wing
Southern Illinois University
Carbondale, IL 62901
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 11 Sep 1997 14:34:13 -1000 (HST)
Subject: Philips 75 TEM free to good home

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It's been cute sitting in the corner, but now this prof. needs the space.
Does anyone want a Philips 75 TEM, 1960s vintage? It's small, not too
heavy, and actually ran until it blew a filament sometime back. I'm not
sure which decade that was... I have the manuals and tools as well.

You would, of course, have to pay packing and shipping.

For more info, drop me a line at tina-at-pbrc.hawaii.edu

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From: Diana_Papoulias-at-nbs.gov (Diana Papoulias)
Date: Thu, 11 Sep 1997 19:54:56 -0600
Subject: autotechnicon to go for parts

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We have an old autotechnicon that could be useful for parts, otherwise
it goes to the scrap heap.

Contact:
Diana_Papoulias-at-nbs.gov




From: ech-at-unixg.ubc.ca (Elaine Humphrey)
Date: Thu, 11 Sep 1997 18:13:08 -0700
Subject: Skin Biopsy-Not

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NK\SKIN\1984

THE PATHOLOGY OF SKIN BIOPSY


When taking a biopsy, careful attention to the following points will ensure
that the histopathologist is quite unable to give any useful information.

1. Paint the skin with a strong antiseptic solution, preferably one that
is deeply coloured (after all, the tissue has to be stained sooner or
later).

2. Rub on the antiseptic vigorously to ensure that the surface layers of
the epidermis are so disturbed as to be histologically uninterpretable.

3. Inject a considerable volume of local anaesthetic solution into the
middle of the area to be biopsied and preferably inject the fluid rapidly.
This will produce a satisfactory degree of tissue distension and
distortion.

4. Seize the area to be biopsied with artery forceps, making sure that the
forceps are clamped firmly enough to crush the tissue. Should the biopsy
be too large to be effectively be dealt with by one pair of forceps then do
not hesitate to use several pairs.

5. The tissue excised should be from the centre of the lesion and should
not include any of the adjacent normal tissue. It is making things too
easy for the Pathologist if the specimen allows the relationship of the
lesion to the adjacent tissue to be seen.

6. When making the excision, two alternative and equally effective
techniques may be considered. Either the biopsy can be only a fraction of
a millimetre thick (thus saving the trouble of cutting sections) or a large
piece of tissue can be excised. In this case make a number of tentative
cuts so that the biopsy is partly cut through in several places. This will
help to make the specimen impossible to orientate for proper sectioning.

7. If you think that the lesion may be an invasion tumour, always remove
it piecemeal with a curette. The Pathologist will then probably not be
able to tell whether it is invasive or not.

8. Before placing the tissue into fixative, ensure that is adequately
covered with blood. This should be done so effectively that after fixation
the specimen is entirely concealed within clot. This ensures that the
Pathologist is kept busy trying to find the specimen and provides a
reasonable chance that every section will be covered with red blood cells
dislodged from the clots during the sectioning process.

9. If the biopsy is to be really uninterpretable, care must now be
exercised in the selection of the container and the fixative. The
container should be very small so that there is room for a minimum of
fixative. Plastic sequestrene bottles (pink label) are admirable for this
purpose. It is quite surprising how much tissue can be compressed into one
of these. Alternatively, use a container with a very narrow neck. A mass
of tissue is often quite soft when freshly excised and can be forced
through a small hole. When fixed the tissue can not be removed from the
bottle without breaking the glass. With luck fragments of glass will then
be driven into the tissue. This lends excitement to the process of section
cutting.

10. The fluid into which the specimen is placed should on no account be a
conventional histological fixative, such as 10% neutral-buffered formol
saline. Plain water or physiological saline will ensure adequate breakdown
of the cells. If available, Stuart's transport medium is even more
effective in producing putrefaction. If such a culture medium is used, the
specimen should be kept on a radiator until dispatch and should be sent to
the laboratory by post (this is especially effective during the summer
months).

11. On no account should the container be labelled with the patient's name
or any other mark of identification. If you can arrange for several
unlabelled containers to arrive in the laboratory on the same day so much
the better. There is an excellent chance that the specimens from different
patients will be confused. An alternative, and equally effective, measure
is to place more than one biopsy in the same container. If they are from
the same patient make sure the pieces of tissue are all of a similar shape
and size. Thus, if one biopsy shows a neoplasm no-one will know which of
the biopsy sites contains the tumour.

12. In the accompanying request form on no account should you provide the
Pathologist with the name of either the patient, yourself or your address.
Otherwise, there will be some means of indexing the specimen and of knowing
where to send the report when eventually prepared.

13. So that the Pathologist shall not be influence or biased in
interpretation, avoid giving any information regarding the age of the
patient, or the site of the biopsy, or the duration and appearance of the
lesion. Above all, never mention your own differential diagnosis.

Finally, one or more of the following stratagems should be used in selected
cases:

(a) Ask for serial specimens, bearing in mind that a specimen 5 mm thick
will yield about a 1000 sections.

(b) Telephone the laboratory frequently for the report. If possible
arrange for the first two calls to reach the laboratory before the
specimens.

(C) Ask for rapid-frozen sections especially on large heavily-calcified
masses that have been present for several years.

Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca





From: Jerome Jasso :      jjasso-at-akron.infi.net
Date: Thu, 11 Sep 1997 22:04:26 -0700
Subject: Re: Storing Karnovsky Fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HASWELL Malcolm wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} I want to supply Karnovsky (modified to 2.5% G and 2.5% "paraformaldehyde")
} to a lab to collect 5mm biopsies over a period of time.
} In the past I have used Karnovsky for up to a week after producing it but I
} am not sure about storing it for 1-2 months. Should I store at 4 deg C or
} consider freezing some (it will be in caodylate with 2.5mM CaCl2)?
}
} Malcolm Haswell
} Electron Microscopy
} University of Sunderland

Malcolm:

I have stored this fixative in glass at room temperature for extended
periods (1-2 months) and have had no problems. Once you see some bottom
ppt. or cloudiness, discard with copious amounts of water. Also be
careful of the formalin conc. in the air. Best regards, Jerome.




From: A. Kent Christensen :      akc-at-umich.edu
Date: Thu, 11 Sep 1997 22:39:04 -0400 (EDT)
Subject: Re: dichromate/osmium

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Grace,

Dalton's dichromate-osmium fixative brings back some nostalgic memories.
I used it to fix rat testicular Leydig cells for my PhD thesis research
(about 1956-58, Harvard Biology), and the results suggested that the
smooth endoplasmic reticulum was tubular, rather than than vesicular, as
was commonly believed at the time. In my subsequent postdoctoral work
with Don Fawcett, opossum Leydig cells fixed with Dalton's dichromate
osmium exhibited tubular smooth ER, while the same material fixed with
osmium-acetate veronal, commonly used at the time, had vesicular SER.
This work was published in 1961 (Christensen, Fawcett 1961, J Biophys
Biochem Cytol 9:653-670).

The reference for the fixative is an abstract: Dalton, A.J. 1955, "A
chrome-osmium fixative for electron microscopy," Anat Rec 121:281. I knew
Jack Dalton, who was at the National Cancer Institute, at NIH in Bethesda.
He and Marie Felix were the first to show the Golgi complex by EM (1954,
as I remember).

Kent

A. Kent Christensen
Department of Anatomy and Cell Biology
Medical Sciences II Building
University of Michigan Medical School
Ann Arbor, MI 48109-0616
akc-at-umich.edu
Tel (313) 763-1287
http://www-personal.umich.edu/~akc/

----------------------------

On Thu, 11 Sep 1997, Grace Kennedy wrote:

} Thanks to all who responded to my osmium pepper question. I now know not
} to osmicate in phosphate but haven't decided what I should use for a
} buffer-I don't think phosphate and cacodylate are compatible so a simple
} switch may be out. Has anyone out there used Dalton's dichromate/osmium? I
} cannot find any reference to anything by Dalton. I do have a formula and
} have used it but am curious to know if anyone else has played with it. Tx
} Grace





From: Phil Dobson :      phil.dobson-at-sawater.sa.gov.au
Date: Fri, 12 Sep 1997 15:39:58 +0930
Subject: Subject: Quenching Auto-fluorescence in EFM

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Our lab is using the fluorescent antibody technique to detect and count
Cryptosporidium and Giardia in water samples. One of the biggest
interference?s with this is the auto-fluorescence of algal cells which is
often brighter than the FITC stained cells we are looking for. This is
particularly inconvenient as we are attempting to automate the
microscopy with the use of Image analysis. Part of our efforts are going
into producing a cleaner final concentrate but it is not always possible to
eliminate all the algae. In view of this has any one come across a
product/method for quenching auto-fluorescence that does not affect the
FITC stain.

Phil Dobson
Australian Water Quality Centre
Hodgson Rd, Bolivar
South Australia 5118
Phone 61 8 8259 0341
Fax 61 8 8259 0228
Email phil.dobson-at-sawater.sa.gov.au






From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Fri, 12 Sep 1997 19:27:20 +0900
Subject: Vignetting?

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Sorry for my layman's question.

I'm translating a microscope manual from Japanese into English and constantly running into sentences that go like "part of the field of view may be cut off" and "image cut-off in peripheral part may occur."

Could anyone tell me how to put them correctly? Should I instead say "the field of view may be vignetted" and "image vignetting may occur" which sound more technical to me but I'm not sure about.

Any suggestion is welcomed. Thanks in advance.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451






From: Ray Hicks :      rh208-at-cus.cam.ac.uk
Date: Fri, 12 Sep 1997 12:32:18 +0100
Subject: Re: Subject: Quenching Auto-fluorescence in EFM

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Hi Phil,

have you considered measuring the emission at two wavelengths? The
autofluorescence of algae should be pretty red, whereas fluorescein is
predominantly green. Subtracting a scaled red image from the corresponding
green image should leave you with only fluorescein fluorescence, presuming
that all of the algae have the same fluorescence spectrum. Alternatively,
if the cells remain discrete in the image, it would be possible to get your
image analysis package to ignore the algae on the basis of size and/or
create a mask in the red image that prevents the algae being counted in the
green one.


Ray


At 3:39 pm +0930 12/9/97, Phil Dobson wrote:
} ------------------------------------------------------------------------
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Ray Hicks
________________________________________________________________________
|University of Cambridge |Tel 01223 330149 |
|Department of Medicine |Fax 01223 336846 |
|Level 5, Addenbrookes Hospital |e-mail {rh208-at-cus.cam.ac.uk} |
|Hills Road Cambridge |Web http://facsmac.med.cam.ac.uk |
|CB2 |ftp server ftp://131.111.80.78 |
|UK | |
|_________________________________|_____________________________________|






From: rgarcia-at-nova.wright.edu
Date: Fri, 12 Sep 1997 08:40:25 -0500 (EST)
Subject: Re: IM Analysis Question

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We currently have the first version of the Buehler image analysis system
and I have nor problems with it. It is a very powerful package and for
all of our Materials analysis needs it performs very well. We have not
upgraded to the latest version wich is supposed to be even better due to
lack of funds. They will be happy to demo it for you if you give them a call.


Roberto Garcia
EMF Manager
Wright State University




From: rgarcia-at-nova.wright.edu
Date: Fri, 12 Sep 1997 08:50:39 -0500 (EST)
Subject: Re: Darkroom Doors and Stainless Steel Sink Suppliers?

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Craig,

Sorry about keeping you waiting. Bernies Photo Center can be
reached at 800 346-8884. They will have verything you need and if I could
make a suggestion splurge on a sodium vapor light, it is worth it. No
more stumbling around the darkroom. They also have the best prices around
on bulk 4X5 film. Good luck with the darkroom.

Roberto Garcia
EMF Manager
Wright State University
Dayton, OH




From: CrushStone-at-aol.com
Date: Fri, 12 Sep 1997 09:20:14 -0400 (EDT)
Subject: Re: Vignetting?

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In a message dated 97-09-12 08:45:19 EDT, Chiba writes:
{ { sentences that go like "part of the field of view may be cut off" and
"image cut-off in peripheral part may occur."
Could anyone tell me how to put them correctly? Should I instead say "the
field of view may be vignetted" and "image vignetting may occur" which sound
more technical to me but I'm not sure about. } }

The phrase "part of the field of view may be cut off" sounds ok to me.
Without an illustration, I do not know what it means. My mental image is
that there is a black part within a photographic image taken with the
microscope from a flash/shutter timing problem, or from blocking of the field
of view by an accessory that is inserted in the optical path, such as the
quarter wavelength plate typically used in a polarizing microscope.

The phrase "image cut-off in peripheral part may occur." could be reworded as
"...the peripheral part of the image may be cut-off." As above, I do no know
really what it means. But I imagine that it means the effect from closing
down the sub-stage iris. I do not think that is really vingetting, a gradual
shading, but it is close to it. If the effect being discussed is from gross
misalignment of the bulb in the illuminator housing, then it could be
vignetting.

Steve Stokowski
Stone Products Consultants
Concrete Petrographers
http://members.aol.com/CrushStone/index.htm







From: Dr. Mark W. Lund :      lundm-at-acousb.byu.edu
Date: Fri, 12 Sep 1997 08:33:07 MST/MDT
Subject: RE: Vignetting?

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Chibasan said:


} Sorry for my layman's question.
} I'm translating a microscope manual from Japanese into
} English and constantly running into sentences that goes
} like "part of the field of view may be cut off" and
} "image cut-off in peripheral part may occur."
}
} Could anyone tell me how to put them correctly?
} Should I instead say "the field of view may be
} vignetted" and "image vignetting may occur" which sounds
} more technical to me but I'm not sure about.
}

I would not use the term "vignette" myself. It is a technical
term that is used in lens design to mean that you have blocked
part of a bundle of rays (hopefully on-purpose to kill the
wild rays that would have not been well focussed, but sometimes
by mistake because a lens element was too small). Although the
word could be used for your purpose, few people know what it
means, and some of them may be confused because of the technical
use. I would just say "the field of view may be reduced," and
"part of the field of view may be cut off." Making it sound
more technical is helpful only for people with trouble falling
asleep :)

best regards
mark

Mark W. Lund, PhD
Director } } Soft X-ray Web page http://www.moxtek.com { {
MOXTEK, Inc.
452 West 1260 North
Orem UT 84057 801-225-0930 FAX 801-221-1121
lundm-at-xray.byu.edu

"The state is good at simple tasks, like killing people and seizing their
wealth. It has far more trouble reaching inside individuals and making them
good." Doug Bandow






From: Dr. Mark W. Lund :      lundm-at-acousb.byu.edu
Date: Fri, 12 Sep 1997 08:33:07 MST/MDT
Subject: RE: Vignetting?

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Chibasan said:


} Sorry for my layman's question.
} I'm translating a microscope manual from Japanese into
} English and constantly running into sentences that goes
} like "part of the field of view may be cut off" and
} "image cut-off in peripheral part may occur."
}
} Could anyone tell me how to put them correctly?
} Should I instead say "the field of view may be
} vignetted" and "image vignetting may occur" which sounds
} more technical to me but I'm not sure about.
}

I would not use the term "vignette" myself. It is a technical
term that is used in lens design to mean that you have blocked
part of a bundle of rays (hopefully on-purpose to kill the
wild rays that would have not been well focussed, but sometimes
by mistake because a lens element was too small). Although the
word could be used for your purpose, few people know what it
means, and some of them may be confused because of the technical
use. I would just say "the field of view may be reduced," and
"part of the field of view may be cut off." Making it sound
more technical is helpful only for people with trouble falling
asleep :)

best regards
mark

Mark W. Lund, PhD
Director } } Soft X-ray Web page http://www.moxtek.com { {
MOXTEK, Inc.
452 West 1260 North
Orem UT 84057 801-225-0930 FAX 801-221-1121
lundm-at-xray.byu.edu

"The state is good at simple tasks, like killing people and seizing their
wealth. It has far more trouble reaching inside individuals and making them
good." Doug Bandow






From: Regina Messer :      messer52-at-eng.uab.edu
Date: Fri, 12 Sep 1997 09:41:20 -0500
Subject: staining monolayer cells

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I have been trying several stains on fixed monolayer fibroblasts. The
cells have been fixed by either formalin, formalin fumes, or
gluteraldehyde. None of the stains are penetrating the cells. Does
anyone have a suggestion?

Regina Messer




From: Sanford Simon :      simon-at-rockvax.rockefeller.edu
Date: Fri, 12 Sep 1997 11:36:45 -0400
Subject: NA of objectives, light collection and image quality

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We just bought a new Olympus inverted microscope for epi-fluorescence. We
were very surprised to find that our 1.2NA water immersion objective gave
BRIGHTER pictures and SHARPER pictures than our 1.4 NA oil immersion
objective. We thought it was a problem with the 1.4 NA objective so the
sales representative brought by:

1) another 1.4 NA oil immersion objective: whose pictures were as poor as
the previous objective
2) a 1.25 NA oil immersion UV objective: whose bright spots were brighter,
whose black regions were darker, and whose contrast was sharper than on the
1.4 NA objective. (The 1.25 NA objective is also much more affordable
objective).

Does anyone have an explanation? One possibility was that the 1.4 NA
objective is NOT a UV objective: we thought that some UV light may be
coming through our 460-490 nm excitation filter causing auto-fluorescence
in the objective. So we put another very sharp 488 nm filter in front of
it and it did not resolve the problem.

In comparing the 1.4 NA oil to the 1.2 NA water objective, shouldn't the
signal be 85% brighter?
- on the excitation side: (1.4/1.2)2=1.36, or 36% more transmission of
excitation
- on the emission side: (1.4/1.2)2=1.36, or 36% more collection of emission
in total: (1.4/1.2)**4=1.85.

So how could the lower NA objectives give brighter sharper signals?

We are collecting our images with a Hamamatsu Cooled-CCD camera. Both the
shutter on the excitation and the collection are under software control, so
we know that data from both objectives are collected under the same
condition. We've used a series of test slides so each objected could be
used on the same image. Any suggestions are welcome.

Thanks,


Sanford M. Simon
Laboratory of Cellular Biophysics
Box 304
Rockefeller University
1230 York Avenue
New York, N.Y. 10021
212-327-8130 (voice)
212-327-8022 (fax)
simon-at-rockvax.rockefeller.edu (e-mail)







From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Fri, 12 Sep 1997 10:50:32 -0500
Subject: Immerse or float EM grids for staining?

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In regards to (i) EM immunocytochemical staining on primary antibodies and
collodial gold
and (ii) uranyl acetate and Pb citrate, I would like to poll the list to
determine how many people either float their EM grids or immerse them in
droplets so that both sides are stained. I have always floated on a 20 ul
drop but have started wondering if I am missing half the action. Comments
about advantages and disadvantages welcomed!

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: Yifan Cheng :      ycheng-at-zen.sb.fsu.edu
Date: Fri, 12 Sep 1997 12:11:35 -0400
Subject: resolution test at a tilt angle, and line resolution of film

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Dear every body,

I have a couple of questions and will appreciate any replies and suggestions.

1) What is the best sample to test the resolution of a TEM at a tilt angle
(} 30degree)?

I am testing the resolution of a CM300FEG microscope. I used oriented gold,
evaperated gold particles, graphitized carbon and so on. There is no problem of
those samples at zero tilt. But after tilt the specimen to 30 degree or higher,
I got only the good resolution (2.3A in case of evaperated gold particle) in
one direction, along the tilt axis. I believed it is not because of the focus
gradient that I could not get the same resolution in both directions at this
tilt angle. I think the samples (evaporated gold particles and graphitized
carbon) may not be the suitable samples for the resolution test of the high
tilt angles (higher than 45 degree and up to 60 degree). I wonder if any of you
has done such test before and has any suggestions on which specimen should be
used in this test and where to get it?

2) Is there any published documentation about the line resolution of Kodak
SO-163 film?

I am writing an paper about the performance of the CM300FEG at low
magnificaiton, and need to know the line resolution of Kodak SO-163 (something
I can cite for). But I could not find any published documentation. Of course, I
called Kodak technique support and of course they didn't give me a clue. There
is a web site called "Bibliography on EM Imaging and Related Technologies"
(http://www.uct.ac.za/depts/emu/imaging/papers.htm), but I can not find any
thing there either about SO-163. The only other related information I got was
from a similar discussion in this board a year ago. But the data about line
resolution found in that discussion were mostly estimated but not from any
published documentation. I wonder if any of you know there is any kind of
published documentation including research paper, technique report or so which
gives the line resolution of Kodak SO-163. (BTW, the topic about the line
resolution has been discussed a year ago and I am sorry to ask it again.)

I appreciate any information or suggestion on my request.

Yifan Cheng

BTW, I am not sure if my email address is already on the list or not. So that I
appreciate also that when you reply this email, send a reply to me directly as
well as to the board. Thanks again.


--
**********************************************************************
* Dr. Yifan Cheng * Phone: +1-850-644-4104 (Office)*
* Institute of Molecular Biophysics * or: +1-850-644-9769 (Lab) *
* Florida State University * Fax: +1-850-561-1406 *
* Tallahassee, FL 32306-4380 * Email: ycheng-at-sb.fsu.edu *
* U.S.A. * http://www.sb.fsu.edu/~ycheng *
**********************************************************************




From: James Mabon :      mabon-at-uimrl7.mrl.uiuc.edu
Date: Fri, 12 Sep 1997 12:10:55 -0500
Subject: Zeiss DSM-960 Column Isoslation Valve

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We have a Zeiss DSM-960 with LaB6. This system has a long history of
problems with the
column isolation valve (manually operated) leaking during specimen
exchanges. Numerous fixes have been tried including slightly larger and
softer o-rings and metal shims to change the height/pressure which the gate
cams to when closed, all to no avail. Has any one experienced similar
problems on a Zeiss or on other instruments and was a fix found.

Thanks,
Jim Mabon
_____________________________________________________
James C. Mabon
Center for Microanalysis of Materials
Frederick Seitz Materials Research Laboratory
104 South Goodwin Avenue
Urbana, Illinois 61801
(217)333-4265 *Fax(217)244-2278
email: mabon-at-uimrl7.mrl.uiuc.edu {that's the letter l}
_____________________________________________________




From: ejb11-at-psu.edu (Edward J. Basgall)
Date: Fri, 12 Sep 1997 13:31:15 -0500
Subject: Re: Storing Karnovsky Fixative

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} HASWELL Malcolm wrote:
} }
} } I want to supply Karnovsky (modified to 2.5% G and 2.5% "paraformaldehyde")
} } to a lab to collect 5mm biopsies over a period of time.
} } In the past I have used Karnovsky for up to a week after producing it but I
} } am not sure about storing it for 1-2 months. Should I store at 4 deg C or
} } consider freezing some (it will be in caodylate with 2.5mM CaCl2)?
} }
} } Malcolm Haswell
} } Electron Microscopy
} } University of Sunderland
}
} Malcolm:
}
} I have stored this fixative in glass at room temperature for extended
} periods (1-2 months) and have had no problems. Once you see some bottom
} ppt. or cloudiness, discard with copious amounts of water. Also be
} careful of the formalin conc. in the air. Best regards, Jerome.

I would recommend purging the vials with nitrogen gas prior to sealing.
Use teflon lined caps, if possible and store at 4C.

cheers

Edward J. Basgall, PhD
The Pennsylvania State University
Surface Chemistry Group ejb11-at-psu.edu
Materials Research Institute Building Ph: 814-865-0493
University Park, PA 16802-7003 FAX: 814-863-0618
http://www.personal.psu.edu/ejb11/






From: Lesley Suzanne Bechtold :      lsb-at-aretha.jax.org
Date: Fri, 12 Sep 1997 14:27:32 -0400
Subject: Mouse Brain Atlas

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To anyone in the know,

I'm trying to find out where I can order a good mouse brain atlas.

Thanks in advance!

Lesley Bechtold





From: Luc Nocente :      ln-at-noesisvision.com
Date: Fri, 12 Sep 1997 14:30:33 -0400
Subject: Re: IM Analysis Question

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} } We will be demoing a Noesis pacckage soon.
}
} Superb but not particularly friendly. Steep learning curve.


Kalman, my records indicate you are using our old version Visilog4.1.3.
The new release is much more powerfull and easier to use. Give me a call
and I'll upgrade you at no cost.

Regards,




----------------------------------------------------------------------------
--------
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com http://www.noesisvision.com
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada
----------------------------------------------------------------------------
--------




From: John Arnott :      ladres-at-worldnet.att.net
Date: Fri, 12 Sep 1997 15:52:16 -0400
Subject: Re: filament life

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NANCY SMITH wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.}
} I would be interested to hear from Philips XL30 & XL40 users
} regarding how long their tungsten filaments last.
}
} Thanks for your input.
} Nancy R. Smith
} Director of Operations
} Microscope And Graphical Imaging Center
} California State University, Hayward
} http://www.csuhayward.edu/SCI/sem


Nancy,

Although we do not use a Philips XL30 or XL40 in our own lab, we have
supplied filaments for years. So based on our experience and comments
from our customers, let me make the following observations:

Some Factors Affecting Tungsten Filament Life

1) quality of vacuum
2) single or multiple user instrument
3) high or low KV
4) height setting of filament
5) oversaturation
6) quality of the Tungsten

Some of our customers report life spans of 50 to 200 hours and beyond.
A normal burnout is when the filament has a bubble on the top of the
broken wire. Look for these indicators when the filament burns out too
quickly:

a) normal burnout where the base is clean could mean there is a good
vacuum but an incorrect height setting or oversaturation.
b) a discolered base could mean a bad vacuum
c) a cracked filament (no bubble) could be a flaw in the wire or a
slight crack in the wire during installation

I hope this is of some value.

Sincerely,

John Arnott
Ladd Research




From: Reinhard Rachel t4534 :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: Fri, 12 Sep 1997 22:56:25 +0200
Subject: Re: resolution test at a tilt angle, and line resolution of film

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resolution test:
no answer from my side.

line resolution of film:
films were compared by Downing and Grano (1982) Ultra-
microscopy 7:381-404
the Kodak 4463, as used in this study, is now the SO163,
as given in a data sheet No P-252 by Kodak.
There is no simple figure for the resolution,
but they measured the modulation transfer function for
different frequencies. Check the paper, it is worth reading.
Regards, Reinhard

- - Dr. Reinhard Rachel {Reinhard.Rachel-at-biologie.uni-regensburg.de}
| )| ) Universitaet Regensburg, Lehrstuhl fuer Mikrobiologie
| \| \ D - 93040 Regensburg (Tel.: xx49-941-943-4534)

http://www.biologie.uni-regensburg.de/Mikrobio/Stetter/Gruppen/rachel.html




From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Fri, 12 Sep 97 16:25:23 CDT
Subject: Vignetting?

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Sorry for my layman's question.

I'm translating a microscope manual from Japanese into English and constantly r
unning into sentences that go like "part of the field of view may be cut off" a
nd "image cut-off in peripheral part may occur."

Could anyone tell me how to put them correctly? Should I instead say "the field
of view may be vignetted" and "image vignetting may occur" which sound more te
chnical to me but I'm not sure about.

Any suggestion is welcomed. Thanks in advance.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451




From: Donald P Robertson :      donald-at-csd.uwm.edu
Date: Fri, 12 Sep 1997 16:30:48 -0500 (CDT)
Subject: subscribe

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subscribe Donald Robertson {donald-at-csd.uwm.edu}





From: Barbara Foster :      mme-at-map.com
Date: Fri, 12 Sep 1997 17:42:53 -0700
Subject: Re: Vignetting?

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Chiba Atsushi wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.}
} Sorry for my layman's question.
}
} I'm translating a microscope manual from Japanese into English and constantly running into sentences that go like "part of the field of view
may be cut off" an
}
} Could anyone tell me how to put them correctly? Should I instead say "the field of view may be vignetted" and "image vignetting may occur"
which sound more tec
}
} Any suggestion is welcomed. Thanks in advance.
}
} Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
} Voice: (+81) 010-045-9451Chiba,
I would go with the simpler language. While vignetting is the
appropriate term, it is less explicit.

Best regards,
Barbara Foster
MME




From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.abbott.com
Date: Fri, 12 Sep 1997 16:19:00 -0500 (CDT)
Subject: Fall Meeting of MMMS

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MARK YOUR CALENDARS NOW!!! EVERYONE IS WELCOME TO ATTEND!

The Fall meeting of the Midwest Microscopy and Microanalysis Society
will be held on Friday, November 7, 1997, at Eli Lilly & Co. in
Greenfield, Indiana (near Indianapolis). The meeting is hosted by
Jeffrey Horn of the Toxicology Research Laboratories at Lilly.

The schedule for the meeting is:

8:30 Welcome and Brief Overview of EM Lab Functions at Lilly - J. Horn,
M.S., Eli Lilly and Company

9:00 Electron Microscopy in Pharmaceutical Drug Development - V.
Meador, DVM/PhD, Eli Lilly and Company

9:30 Everything You Always Wanted to Know About Molecular Morphology
(But Were Afraid to Ask) - the Basics of In Situ Labeling Methods - J.
Fagerland, Ph.D., Abbott Laboratories

10:00 Coffee Break

10:30 Ultrastructural Evidence in Legal Cases, N. Cheville, DVM/PhD,
Iowa State University

11:30 Lunch (Provided)

12:45 Tour Eli Lilly & Co. Toxicology Facility

2:00 Use of SEM for Selecting Therapeutic Candidate in Various in vivo
Thrombosis Models - G. Sandusky, DVM/PhD Indiana University

2:30 Diagnostic Electron Microscopy in the Hospital Setting - M. Goheen,
M.S. Indiana University Hospitals

3:00 Coffee Break

3:30 Digital Imaging for Dummies - J. Gagne, M.S., Abbott Laboratories

4:15 Conclusion

Meeting announcements, maps, and registration instructions will be sent
to members of MMMS in U.S. Mail soon. Anyone else can receive them by
contacting me at (847) 935-0104 or via email at
jane.a.fagerland-at-abbott.com.

HOPE TO SEE YOU ALL IN INDY!





From: Carlos E. Barbosa :      grial-at-satlink.com
Date: Fri, 12 Sep 1997 22:06:36 -0300
Subject: UV microscopy

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Does someone can say me what type of lamp have I to use for performing
UV micropscopy of thin-sections of rocks.
Thank you in advance!

Carlos Barbosa





From: ScottE57-at-aol.com
Date: Fri, 12 Sep 1997 21:45:48 -0400 (EDT)
Subject: Re: NA of objectives, light collection and image quality

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Sanford, first are you speaking of the differences with objectives as seen
through eyepieces or with the CCD camera? if the problem is with the CCD
camera one problem is probably IR leakage onto CCD with one objective and not
the other. The NA of the lens should be taken as a measure of resolution
capability not amount of light transmission, though with two objectivesof
exact same type of glass and design then the NA can be used to see who
collects more light. But when you start to compare a PlanApo to PlanNeofluar
then the glass and lens design insdie objective may have drastically
different spectral properties, especially outside visible range (Up in IR
} 850nm) this is what will cause a low contrast soft image.

Another likely possiblity is that the lower the NA the more depth of field
the objective will have and thereby when looking at a 3-d object have a
sharper edge as the fluorecesence is coming from above and below focal plane
(This is why confocal looks so good and why most quality microscopes will
offer, and you should be purchasing an objective with an iris diaphram -- or
have body of scope that has it built into body--aperture diaphramfor epi
path-- to cut down on NA to cut down on bloom in sample. (your flourescent
cell)

To check yourself put both objective on upright scope with high resolution
condenser and look at a very thin {5micoron sectionsof H&E stain tissue. at
this point the higher NA should be clearer and sharper then the 1.25NA . But
other than BF you can see that nore NA is not always better.

Scott E. Berman
Advanced Imaging Concepts, Inc.
(609) 921-3629 x26
Princeton, NJ
scotte57-at-aol.com




From: ScottE57-at-aol.com
Date: Fri, 12 Sep 1997 23:18:23 -0400 (EDT)
Subject: Re: NA of objectives, light collection and image quality

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Sanford, first are you speaking of the differences with objectives as seen
through eyepieces or with the CCD camera? if the problem is with the CCD
camera one problem is probably IR leakage onto CCD with one objective and not
the other. The NA of the lens should be taken as a measure of resolution
capability not amount of light transmission, though with two objectivesof
exact same type of glass and design then the NA can be used to see who
collects more light. But when you start to compare a PlanApo to PlanNeofluar
then the glass and lens design insdie objective may have drastically
different spectral properties, especially outside visible range (Up in IR
} 850nm) this is what will cause a low contrast soft image.

Another likely possiblity is that the lower the NA the more depth of field
the objective will have and thereby when looking at a 3-d object have a
sharper edge as the fluorecesence is coming from above and below focal plane
(This is why confocal looks so good and why most quality microscopes will
offer, and you should be purchasing an objective with an iris diaphram -- or
have body of scope that has it built into body--aperture diaphramfor epi
path-- to cut down on NA to cut down on bloom in sample. (your flourescent
cell)

To check yourself put both objective on upright scope with high resolution
condenser and look at a very thin {5micoron sectionsof H&E stain tissue. at
this point the higher NA should be clearer and sharper then the 1.25NA . But
other than BF you can see that nore NA is not always better.

Scott E. Berman
Advanced Imaging Concepts, Inc.
(609) 921-3629 x26
Princeton, NJ
scotte57-at-aol.com




From: Barbara Foster :      mme-at-map.com
Date: Sat, 13 Sep 1997 15:37:24 -0700
Subject: Re: UV microscopy

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Carlos E. Barbosa wrote:
}
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} Does someone can say me what type of lamp have I to use for performing
} UV micropscopy of thin-sections of rocks.
} Thank you in advance!
}
} Carlos BarbosaDear Carlos,

Both high pressure mercury arcs and xenon arcs show good emission in the
UV. One caution: most conventional optics do not transmit below about
365-380 nm. Talk to your microscope vendor about quartz optics for all
relevant components.
I assume that you will be doing fluorescence, since humans cannot see
into the UV (although I am told that those of us who have had cataract
operations see further into the near UV than those of us with convention
eyes) *and* of course, direct viewing of UV light can literally fry the
delicate tissue in the eye.
With UV fluorescence from a thin section, the job is a bit easier, but
you will need quartz optics throughout the illumination system ,
including the objective. You may also want to test the glue which is
holding your thin section to its substrate, to make sure that it is not
autofluorescent, creating undesirable background.

Let me know how things work out.

Barbara Foster
President
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email:mme-at-map.com
---------------------------------------------------------------------------------------------------------------------------------
********** Microscopy/Microscopy Education **********
America’s First National Consortium of Microscopy Experts
Specializing in Customized, On-site Training
in all areas of Microscopy, Sample Prep, and Image Analysis




From: Long Liang :      lian6377-at-utdallas.edu
Date: Sat, 13 Sep 1997 23:04:14 -0500 (CDT)
Subject: SEM applications in the Semiconductor field

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Does anyone know of any new books detailing SEM applications
(voltage contrast, EBIC, etc) in the Semiconductor field ?

Thanks.

James L.


********* Have a good day ***************





From: Richard Lander :      richard.lander-at-stonebow.otago.ac.nz
Date: Mon, 15 Sep 1997 08:38:28 +1200
Subject: Re: Immerse or float EM grids for staining?

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Thomas,
As I understand for this type of staining, it depends on whether the grids
are 'formvar' coated or not. If the grids that you are using are coated in
some way, even if you did immerse them into solutions, the sections will
only be stained on one side anyway. So there is no advantage in immersing
the grids.

However, if the sections are on uncoated grids, then immersion into the Ab
sols will give you greater staining, (both sides!)

Hope this helps,

Rich.


-----------------------------------------------------------------------
Richard Lander
Electron Microscope Technician
South Campus Electron Microscope Unit
Otago School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand.
Tel. National 03 479 7301 Fax. National 03 479 7254

"Southernmost EM Unit in the World!"
------------------------------------------------------------------------






From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Mon, 15 Sep 1997 11:15:19 +1200
Subject: Re: Immerse or float EM grids for staining?

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=46or both immuno staining and U acetate/Pb citrate, I've always immersed.
In 25 =B5l drops for the former and in a multigrid staining thing for the
latter. THe immersion for immuno was to pick up as much stain as possible
on a structure that was about half as thick as an EM section. Oh, I was
using uncoated grids, of course.


Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Melbourne, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: colin.veitch-at-dwt.csiro.au
Date: Mon, 15 Sep 1997 16:07:38 +1000
Subject: Help: Instructions for a Critical Point Dryer

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Hi All,

For reasons best known to ourselves, we are attempting to resurrect an
OMAR SPC 900/EX critical Point Dryer. It has not been used in some time
and none of us can recall using it, let alone how it was used!! Of
course, the manuals have long since disappeared (presumably to the same
place that odd socks and pens go) and we need to get it going. Does
anybody out there have a manual that they could fax us or even an idea
of the pressures and times we should be using with this little beast?
Any help would be gratefully accepted!

Many thanks,

Colin V.

Colin J. Veitch
Instrumentation Scientist
CSIRO Division of Wool Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-dwt.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811






From: Tamara Bloomer :      bloomer-at-ameslab.gov
Date: Mon, 15 Sep 1997 07:41:57 -0500 (CDT)
Subject: Auger of Charging materials

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Good Morning all,

I work with a LaB6 Auger in a university environment and have been given a
project to identify contaminants on and just below the surface of a
leadframe (Au on epoxy). I can overcome the charging prior to depth
profiling by dropping to low voltage (2Kv or Less). Once I start ion
sputtering for short times the charging is enormous and continuous. Any
suggestions would be appreciated.

Sincerely,










Tamara E. Bloomer
Assistant Scientist
Ames Laboratory
137 Wilhelm Hall
Ames, IA 50011
(515) 294-2564





From: Audette, David :      deaudette-at-corp.olin.com
Date: Mon, 15 Sep 1997 08:07:00 -0500
Subject: RE: SEM applications in the Semiconductor field

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James L.

The text "Advanced Scanning Electron Microscopy and X-Ray Microanalysis"
by Newbury, Joy, Echlin, Fiori, and Goldstein, 1986, Plenum, has a
chapter on characterization of semiconductors which covers EBIC and
voltage contrast.

regards,

Dave Audette
Olin Research Center
Cheshire, CT 06410 USA
(203)- 271-4272
deaudette-at-corp.olin.com





From: EUGENE GORDON :      MEDJET-at-worldnet.att.net
Date: Mon, 15 Sep 1997 09:20:45 -0400
Subject: Unsubscribe

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Please unsubscribe




From: Greg :      greg-at-umic.sunysb.edu
Date: Mon, 15 Sep 1997 10:41:53 -0400
Subject: Re: Help: Instructions for a Critical Point Dryer

Contents Retrieved from Microscopy Listserver Archives
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colin.veitch-at-dwt.csiro.au wrote:
}
} Hi All,
}
} For reasons best known to ourselves, we are attempting to resurrect an
} OMAR SPC 900/EX critical Point Dryer. It has not been used in some time
} and none of us can recall using it, let alone how it was used!! Of
} course, the manuals have long since disappeared (presumably to the same
} place that odd socks and pens go) and we need to get it going. Does
} anybody out there have a manual that they could fax us or even an idea
} of the pressures and times we should be using with this little beast?
} Any help would be gratefully accepted!
}
Hi Colin:
Times have to be determined by trial and error. It depends upon the
sample. As to temperature, the chamber must be cooled to between 15-20C
in order for there to be liquid CO2. The solvent used to dehydrate the
sample must be removed and this is done with repeated flushing of the
chamber. When this is done the temp is raised to above 32C(36C just to
be sure) pressure will be around 1200lbs. At this point the critical
point has been reached and passed and pressure can be slowly lowered.
Another option is Hexamethlydisilazane(HMDS). This is a liquid that
can be used in place of CPD. After dehydration HMDS is subistuted
with several changes and then allowed to air dry. It gives good
results. You will have to try it on your sample

Hope this helps. Good luck.
THE OPINIONS HERE ARE NOT THOES OF THE FACILITY
Greg Rudomen
Greg-at-umic.sunysb.edu
University Microscopy Imaging Center
S.U.N.Y. Stony Brook
Stoy Brook, NY 11794-8088




From: Long Liang :      LLIANG-at-mail.arco.com
Date: Mon, 15 Sep 1997 12:19:07 -0500
Subject: MSA/MAS meeting proceedings

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Dear Microscopists,

This year I didn't attend the MSA/MAS annual meeting.
Does anyone have information about how to order a copy of the proceedings
and how much ?

Thanks a lot.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX

E-mail: lliang-at-mail.arco.com






From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Mon, 15 Sep 1997 10:53:15 -0700
Subject: Remove -Reply

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Sirs: please remove my name from your list.




From: Regina Messer :      messer52-at-eng.uab.edu
Date: Mon, 15 Sep 1997 13:45:27 -0500
Subject: thanks

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Thank you to everyone who applied to my question regarding staining
monolayer fibroblast. You've been very helpful.

Regina Messer




From: Lesley Weston :      lesley-at-unixg.ubc.ca
Date: Mon, 15 Sep 1997 14:02:33 -0700 (PDT)
Subject: Re: dichromate/osmium

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I think it is O.K. to follow phosphate with cacodylate. We do so
routinely, and don't seem to have any problems.
Lesley Weston.


On Thu, 11 Sep 1997, John Chandler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} } Thanks to all who responded to my osmium pepper question. I now know not
} } to osmicate in phosphate but haven't decided what I should use for a
} } buffer-I don't think phosphate and cacodylate are compatible so a simple
} } switch may be out. Has anyone out there used Dalton's dichromate/osmium? I
} } cannot find any reference to anything by Dalton. I do have a formula and
} } have used it but am curious to know if anyone else has played with it. Tx
} } Grace
}
} In a former life, I used Dalton's fixative for immersion fixation of
} vertebrate retina. I don't remember all the details, but the results were
} very good. I don't remember this fix being widely used, though.
}
} John
} chandler-at-lamar.ColoState.EDU
}
}
}





From: John Grazul :      grazul-at-BIOLOGY.RUTGERS.EDU
Date: Tue, 16 Sep 1997 08:44:04 EDT
Subject: Balzers Piranni Gauges, alternate sources

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All,

I need a TPR 010 Piranni gauge for my Balzers BAF 301 Freeze etcher.
Techno Trade does have them for $250.00 each...which is ok on an
unlimited budget. Has anyone else gotten these elsewhere? Is there
an aftermarket brand that I might use? And yes I have cleaned many a
TPR 010 {I have a real good technique...} but after around four
cleanings they act very strange. Any help, advise, or berations
are welcome.

John Grazul
Rutgers University
Electron Imaging Facility




From: george.braybrook-at-ualberta.ca (George Braybrook)
Date: Tue, 16 Sep 1997 08:14:16 -0600
Subject: Re: Vignetting?

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Dear Mr. Chiba,

All manuals should follow the "KISS" principle! ie: Keep It
Stupidly Simple!!!! Forget about whether it sounds "technical" or not, just
make sure the directions are abundantly clear to a 5 year old child and
researchers around the world will applaud you.



}
} Sorry for my layman's question.
}
} I'm translating a microscope manual from Japanese into English and
} constantly running into sentences that go like "part of the field of view
} may be cut off" and "image cut-off in peripheral part may occur."
}
} Could anyone tell me how to put them correctly? Should I instead say "the
} field of view may be vignetted" and "image vignetting may occur" which
} sound more technical to me but I'm not sure about.
}
} Any suggestion is welcomed. Thanks in advance.
}
}
} Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
} Voice: (+81) 010-045-9451

Cheers
:)
George
Department of Earth and Atmospheric Sciences
University of Alberta
Edmonton, Alberta T6G 2E3
Canada
ph: 403-492-5746
fax: 403-492-2030






From: bozzola-at-siu.edu (John J. Bozzola)
Date: Tue, 16 Sep 1997 10:34:07 -0600
Subject: nearly photographic prints

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Anyone aware of a "nearly" conventional photographic process for
development of prints made from enlarged negatives? Examples: stabilization
processors that use heat to develop sensitized papers OR (less desirably)
concentrated chems as in the Kodak Ektamatic-like scheme.

Unfortunately, the heat-developed papers use mercury and silver and will be
phased out shortly while the Ektamatic-type stabilization processors
generate liquid wastes. The ideal situation would involve conventional negs
enlarged onto a paper that could be processed using some sort of dry (or
nearly so process) AND yielding photographic quality images.

Now, digital imaging people please don't jump all over me, because
sometimes digital images and prints just do not offer adequate rendition of
the information -- without spending big bucks. Right now, we are working
with dense, highly contrasted negatives (difraction patterns, high contrast
and thick specimens) and the quality of the digital images (direct image
capture or scanning of TEM negs) is inadequate. My own idea would be to
have a system akin to a xerographic process wherein one would enlarge a
negative onto a high quality paper that would then be "developed" in a
manner similar to a dry copier probably using toner powders. I believe all
of these components currently exist but have not been put together - to my
knowledge.




####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Neckers Building, Room 146 - B Wing
Southern Illinois University
Carbondale, IL 62901
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: zhang-at-cvlab.harvard.edu (Dorothy Zhang)
Date: Tue, 16 Sep 1997 10:17:29 -0500
Subject: LM-how to cut serial sections of GMA embedded tissue and stain with Oil Red O?

Contents Retrieved from Microscopy Listserver Archives
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Dear reader,
If you have any idea to cut serial sections of GMA embedded tissue and
stain with Oil Red O?






From: edelmare-at-casmail.muohio.edu
Date: Tue, 16 Sep 1997 12:16:53 -0500
Subject: Re-coating TEM Screens

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O.k., I know this has been discussed MANY times before, but I I
didn't save all the relavent messages.

Looking for two things:

(1) Refurbished / replacement screen for a JEOL 100-S (hey it still
works fine)

(2) Looking for places to recoat our old 100s screen. I believe
that SPI still does it, and will call soon (I did save your message
Chuck). Grant Sci Corp doesn't seem to exist any more. Any others
to consider? Any comments god or bad?

Thanks.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu


"640K ought to be enough for anybody."
-- Bill Gates, 1981




From: joyce craig :      bafpjec-at-csu.edu
Date: Tue, 16 Sep 1997 11:19:24 -0700
Subject: block storage

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We have 10 years of blocks filed and stored in small plastic boxes from
Althor Products. Our last purchase order was returned: no forwarding
address. Does anyone know how to contact these people or of another
source for those 1 3/4 x 7/8 x 3/4" boxes with snap-on lids?
Thanks
Joyce Craig
Chicago State University




From: Lindsey :      phantom-at-ou.edu
Date: Tue, 16 Sep 1997 11:58:14 -0500
Subject: (no subject)

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please take me off this list. I don't want on it




From: giba-at-puccini.crl.umn.edu
Date: Tue, 16 Sep 1997 12:42:32 -0500
Subject: SEM Job Opportunity

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SEM Opportunity

-Electron microscopy operator needed

-2 or 4 yr science degree

-Scanning electron microscope exp 2yrs+

Person will be running samples thru SEM, checking for flaws and
contaminations.

Opportunity available in Minneapolis/St. Paul, Minnesota USA area
immediately! Please contact ASAP.

Paul Drange - Aerotek Scientific Staffing
Phone - 1-800-716-8556 ext. 6615
e-mail - pdrange-at-aerotek.com





From: Susan Carbyn :      CarbynS-at-em.agr.ca
Date: Tue, 16 Sep 1997 13:38:44 -0400
Subject: Cleaning an embedding oven

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We inherited an old embedding oven that is caked in resin. Does anyone
ever clean out their embedding oven? If so, what is used to do this?

I am not so concerned about the cleanliness of the oven, but rather the
problems it seems to be causing when things start sticking to the oven.
There is a plastic petri dish with old desiccant in it that has stuck itself
onto the bottom of the oven, with no way to easily dislodge it.

Any suggestions or comments would be greatly appreciated.

Susan

Susan Carbyn
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
Kentville, Nova Scotia B4N 1J5
Canada

Phone (902) 679-5566
Fax (902) 679-2311

E-mail: carbyns-at-em.agr.ca




From: nancy buening :      buening-at-topaz.ucdavis.edu
Date: Tue, 16 Sep 1997 11:23:53 -0700
Subject: TEM Ion thinning vs. tripod polishing

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I am making TEM samples of modern and fossil brachiopod (a bivalved marine
invertebrate) shells composed of calcite. I was intending to use an ion
milling device (argon) to thin the samples. However, it has been suggested
that a tripod polisher would be less time consuming and provide a larger
"viewing" area.

Has anyone had experience using a tripod polisher on carbonate materials?
Would tripod polishing produce more defects in the crystallographic
structure than ion thining? My intent, if possible, is to evaluate
diagenetic alteration by observing crystallographic defects.

Thanks.

Nancy Buening

Nancy Buening E-mail: buening-at-geology.ucdavis.edu
Department of Geology Phone: (916) 752-0350
University of California Fax: 916 752-0951
Davis, CA 95616






From: zhang-at-cvlab.harvard.edu (Dorothy Zhang)
Date: Tue, 16 Sep 1997 14:16:08 -0500
Subject: Re: LM-how to cut serial sections of GMA embedded tissue and stain with Oil Red

Contents Retrieved from Microscopy Listserver Archives
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Julian,
I didn't expect the reply so quick. I certainly try that. Thanks so much.
Regarding serial sectioning, you didn't mention what kind of knife you used
for GMA sectioning. Is
tungsten or glass knife? Which is better? Regular Oil Red O staining
procedure won't work with GMA section. Why? The reason we choosing GMA is that
the morphology is better than frozen tissue. Do you have suggestion about
this?
Dorothy






From: pdf-at-fullam.com
Date: Tue, 16 Sep 1997 14:52:31 -0500
Subject: Re: Re-coating TEM Screens

Contents Retrieved from Microscopy Listserver Archives
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We recoat screens in our lab. Please contact us for pricing.

Dianne
Ernest F. Fullam, Inc.

O.k., I know this has been discussed MANY times before, but I I
} didn't save all the relavent messages.
}
} Looking for two things:
}
} (1) Refurbished / replacement screen for a JEOL 100-S (hey it still
} works fine)
}
} (2) Looking for places to recoat our old 100s screen. I believe
} that SPI still does it, and will call soon (I did save your message
} Chuck). Grant Sci Corp doesn't seem to exist any more. Any others
} to consider? Any comments god or bad?
}
} Thanks.
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
}
} "640K ought to be enough for anybody."
} -- Bill Gates, 1981
}
}
Ernest F. Fullam, Inc.
Phone: (518) 785-5533 FAX: (518) 785-8647
E-Mail: pdf-at-fullam.com

************************************************************
* Complete on-line product listing: http://www.fullam.com/ *
************************************************************





From: edelmare-at-casmail.muohio.edu
Date: Tue, 16 Sep 1997 17:27:29 -0500
Subject: Apology! Grant Scientific Exists!

Contents Retrieved from Microscopy Listserver Archives
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I apologize to Grant Scientific Corp for passing apparent
misinformation, for indeed Grant Scientific does exist (as it has
since 1975 I have been informed). I have spoken with the good folks
at Grant, and they have been able to provide me some very useful
information regarding TEM Screen re-coating.

I wish to make it all known to the listserver goupr that Grant does
exist and can be contacted at: 803-829-2841.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu


"640K ought to be enough for anybody."
-- Bill Gates, 1981




From: nancy buening :      buening-at-topaz.ucdavis.edu
Date: Tue, 16 Sep 1997 14:24:53 -0700
Subject: Apology! Grant Scientific Exists!

Contents Retrieved from Microscopy Listserver Archives
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To whomever,

I am replying to my subscription request. All information is correct.

Nancy Buening

Nancy Buening E-mail: buening-at-geology.ucdavis.edu
Department of Geology Phone: (916) 752-0350
University of California Fax: 916 752-0951
Davis, CA 95616






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 16 Sep 1997 13:16:03 -1000 (HST)
Subject: Re: Cleaning an embedding oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Tue, 16 Sep 1997, Susan Carbyn wrote:

} We inherited an old embedding oven that is caked in resin. Does anyone
} ever clean out their embedding oven? If so, what is used to do this?

I used to chisel away at the stuff, and try to use various solvents to
clean off the glass door without dissolving my gloves, with limited
success. One day I came into the lab and, lo! the oven was clean. Turns
out someone had cleaned it out with our putty knife WHILE IT WAS HOT!
Duh. She said it was pretty easy. Be careful of all surfaces. Wear
gloves. Worry about toxicity. Disregard me if anyone says this is not
recommended.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From: Robert H. Olley :      R.H.Olley-at-reading.ac.uk
Date: Wed, 17 Sep 1997 09:35:10 +0100 (BST)
Subject: Re: Cleaning an embedding oven

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 16 Sep 1997, Susan Carbyn wrote:

}
} We inherited an old embedding oven that is caked in resin. Does anyone
} ever clean out their embedding oven? If so, what is used to do this?
}

I am not sure what kind of resin is involved, but once I had to clean out
a 5 litre three-neck flask that had been used to synthesize alkyd resins -
these are basically phthalic polyesters crosslinked through
polyunsaturated fatty acids. There was a caked-on film that would not
yield to concentrated sulphuric acid, tetrahydrofuran, or any of the usual
things. I put some .880 Ammonia in the bottom of the flask, blocked the
necks with cotton wool, and left it overnight, and next morning the film
had swollen and fallen away and could be yanked out with tongs or
whatever.

Ammonia vapour is very effective with polyester based resins because of
(a) it basic nature and (b) most important, its small molar volume.

If, on the other hand, your resin is an epoxy, it might be better to put a
dish of methylene chloride (dichloromethane) in the bottom, seal the oven,
and go away overnight. Methylene chloride is the basis of most commercial
paint strippers.

The use of vapour technique does make for much less messy operation. Once
the film is loosened, strong detergent should be good enough for
scrubbing.

However, that PLASTIC PETRI DISH would probably turn into a gooey mess
with the methylene chloride vapour, so try the ammonia first. (Also
consider, does your oven seal with an O-ring?)

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+






From: ebs-at-ebsciences.com
Date: Wed, 17 Sep 1997 07:27:18 EST
Subject: Oil Red O for GMA

Contents Retrieved from Microscopy Listserver Archives
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Dorothy and fellow microscopists,

I have a procedure for Oil Red O staining of GMA sections which was sent to
me by Bob Schoonhoven. I haven't tried it myself.

Stain: Oil Red O
Indication: Demonstrate lipids
Solutions:
1) 60% aqueous triethyl phosphate
2) 0.5% Oil Red O solution
0.5g Oil Red O (CI 26125)
100 ml 60% aqueous triethyl phosphate
Filter before use
3) Celestin Blue
0.5g celestin blue B
100 ml 5% aqueous ferric ammonium sulfate
Boil gently 2-3 minutes; cool to room temperature; filter; and
add 12 ml glycerol
Filter before use
Procedure:
1) Rinse briefly in 60% triethyl phosphate
2) Stain 5-20 minutes in Oil Red O solution
3) Rinse 1-2 seconds in 60% triethyl phosphate
4) Rinse well in distilled water
5) Counterstain in Celestin Blue 15 minutes
6) Rinse well in distilled water
7) Mount in glycerin jelly or other water-soluble mount
Results:
lipids: red-orange
nuclei: blue
Reference:
Feldman, A.T. and Dapson, R.W., "Relative Effectiveness of Various Solvents
for Oil Red O," _Medical Laboratory Technology_, Vol. 31:335-341, 1974.

Disclaimer: Energy Beam Sciences manufactures the JB-4 and JB-4A microtomes
for sevtioning plastic-embedded tissue, and sells GMA kits.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: edelmare-at-casmail.muohio.edu
Date: Wed, 17 Sep 1997 08:30:39 -0500
Subject: CORRECTION: Grant Sci Phone Number.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

(Being dislexic last night, here's the correct and functional phone
number.)

Grant is still going strong at:

Grant Scientific Corp.
1385 Rock Island Rd.
Gilbert, SC 29054

803 892 2841 phone
803 892 2441 fax



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu


"640K ought to be enough for anybody."
-- Bill Gates, 1981




From: R-Brooks Corl at ~CC003DPO
Date: 9/13/97 5:42PM
Subject: Re[2]: Vignetting?

Contents Retrieved from Microscopy Listserver Archives
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The vignetting I most frequently see is a cut-off of the image at the
corners due to the microscope's camera tube. This can commonly be
caused by using an adapter (for example, a C-mount adapter for the
digital or video microscope camera) with too wide a field of view.
The descriptions you cite in the Japanese text could be describing
this or other phenomena. You'd need to know more from the original
author(s) to be sure.
Brooks Corl
Senior Applications Manager, Polaroid Corporation
E-mail: corlb-at-polaroid.com


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

In a message dated 97-09-12 08:45:19 EDT, Chiba writes:
{ { sentences that go like "part of the field of view may be cut off" and
"image cut-off in peripheral part may occur."
Could anyone tell me how to put them correctly? Should I instead say "the
field of view may be vignetted" and "image vignetting may occur" which sound
more technical to me but I'm not sure about. } }

The phrase "part of the field of view may be cut off" sounds ok to me.
Without an illustration, I do not know what it means. My mental image is
that there is a black part within a photographic image taken with the
microscope from a flash/shutter timing problem, or from blocking of the field
of view by an accessory that is inserted in the optical path, such as the
quarter wavelength plate typically used in a polarizing microscope.

The phrase "image cut-off in peripheral part may occur." could be reworded as
"...the peripheral part of the image may be cut-off." As above, I do no know
really what it means. But I imagine that it means the effect from closing
down the sub-stage iris. I do not think that is really vingetting, a gradual
shading, but it is close to it. If the effect being discussed is from gross
misalignment of the bulb in the illuminator housing, then it could be
vignetting.

Steve Stokowski
Stone Products Consultants
Concrete Petrographers
http://members.aol.com/CrushStone/index.htm
---------------------------------- Forwarded ----------------------------------




From: Klaus-Ruediger Peters :      Peters-at-BSAC.UCHC.EDU
Date: Wed, 17 Sep 1997 10:22:07 -0500
Subject: MSA-98: Applied Image Processing: What Can It Do For You?

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Message-Id: {v03007800b00bcf0c3033-at-[155.37.2.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Are you into digital? Please help to reevaluate digital image processing
for the development of a program for next year's MSA-98 meeting (Follow-up
and Summary will be placed here):

"Applied Image Processing: What Can It Do For Digital Imaging"

If you like to help, type your reply directly into this message and send it
to me at

Klaus-Ruediger Peters {Peters-at-bsac.uchc.edu}

Thanks for your help and voicing your opinion. Klaus
****************************************************************************

What is important, what are you using, what deserves more attention, what
has "practical value" and may represent one of the topics? What is of your
interest? What can't you do but like to? Please, hit "REPLY" now, then
"PASTE" my address into "TO:" and just fill in your Spontaneous thoughts on:

-----------------------------------Being Digital---------------------------
Digital image handling (Shifting data, formatting, labeling, annotating,
8-bit to 16-bit/per channel)



----------------------------------Trying Digital-----------------------------
Digital darkroom (Printing images as hard copies on paper, overheads and
slides)



----------------------------------Doing Digital-----------------------------
Digital image display and "enhancement" (Presenting the desired information)



---------------------------------Using Digital------------------------------
Data quantification (Finding and measuring the desired information)



----------------------------Other topic of interest?------------------------


Images are as diverse as their usages. But, what are the common concepts
and how good do they apply to the common daily imaging tasks? Any possible
contributions from yourself to this MSA 1998 program?


Thanks for your initiative and for your help. Klaus






From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Wed, 17 Sep 1997 10:12:53 -0500
Subject: MMS Fall meeting Tommorrow

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The Minnesota Microscopy Society FALL BUFFET DINNER & TALK will take place
Tommorrow, SEPTEMBER 18, 1997 from 5:30 - 8:00 PM at
The Campus Club, University of Minnesota
Minneapolis, East Bank Campus

SPEAKER: William P. Wergin
Agricultural Research Service,U.S. Dept. of Agriculture, Beltsville, MD

TOPIC: "THE MICROSCOPY OF SNOW:"
"The 3-D Structure and Metamorphoses of Snow and Ice Crystals as Revealed by Low
Temperature SEM".
For more details see our website at http://resolution.umn.edu/MMS/

We hope to provide a pleasant evening during which microscopists will be moved
to renew or begin memberships in MMS, MSA and/or MAS.

Program
5:30-6:00 Wine, Cider & Cheese Social
6:00-7:00 Buffet Dinner.
7:00-8:00 Talk
The Buffet Dinner is $12 per MMS member, payable at the door.
(non-member fee is $22, includes new membership, to attend without becoming
member, $15.)
STUDENTS: NEW FEATURE THIS YEAR: Current student members or new student members
- $5.00 membership fee payable at the door- will receive a complimentary buffet
dinner courtesy of MMS and sponsoring vendors.

Please make an advance reservation by contacting:

Mike Coscio (612)569-1331, 569-1284 FAX, mike.coscio-at-medtronic.com
Stuart McKernan, (612)626-7942, 626-7530 FAX, stuartm-at-maroon.tc.umn.edu

Parking is available behind the Union in the East River Road Ramp (connected by
walkway to Union) for $2.50 (per day rate), at the Radisson Ramp on Washington
Ave. S.E., a block east of the Union, and at other Minneapolis Campus locations


__________________
Stuart McKernan stuartm-at-tc.umn.edu
Microscopy Specialist
CIE Characterization Facility, University of Minnesota Phone: (612) 626-7594
100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590





From: Delilah Wood :      wood-at-pw.usda.gov
Date: Wed, 17 Sep 1997 08:57:35 -0700
Subject: immunolabel of protein in potato

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A collegue contacted me asking for information on labeling of a small
protein in potato tuber. Apparently, she is having problems with the potato
starch disintegrating in the electron beam. Do any of you have any
suggestions about how to retain antigenicity and, at the same time, attain
good (or reasonable) fixation. If so, please contact her offline at the
email address below as she is not yet a member of this list.

Her name is Bonnie Compas: email address is: becompas-at-csupomona.edu


Thank you,

De Wood




*****************************************

Delilah F. Wood
United States Department of Agriculture
Western Regional Research Center
800 Buchanan Street
Albany, CA 94710

Tel: 510.559.5653
Fax: 510.559.5777
Email: wood-at-pw.usda.gov




From: Dr. Gary Gang REN :      gangren-at-scripps.edu
Date: Wed, 17 Sep 1997 08:56:23 -0700 (PDT)
Subject: screen size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Did anybody know the size of large and small viewing screen of CM120
} and CM200/FEG. Your information will be greatly appreciated!
} gary
}
}
} +--------------------------------------------------------------------+
} | Gary Gang REN, Ph.D. |
} | Department of Cell Biology #MB219 Email: gangren-at-scripps.edu |
} | Mail Stop MB21 Tel: (619) 784 9815 (O) |
} | The Scripps Research Institute (619) 546 1585 (H) |
} | 10550 N. Torrey Pines Road Fax: (619) 784 9927 |
} | La Jolla, CA 92037, USA http://leonardo.scripps.edu/ren |
} +--------------------------------------------------------------------+
}
}
}





From: Kristen Wagschall :      kwagscha-at-aerotek.com
Date: Wed, 17 Sep 1997 13:30:31 -0400
Subject: Job Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for a person to fill a 1 year surface chemist position at a
petroleum company in Beacon, NY (Dutchess County). The person must have
Ultra High Vacuum, X-Ray Spectoscopy (XPS) and Scanning Electron
Microscopy (SEM)or AES experience. The chemist will be doing sample
prep and intro, analysis, and running data. It is an applied research,
product related position. Please e-mail me at kwagscha-at-aerotek.com or
call 1-800-973-1518 ext. 1045 if interested.
Kristen Wagschall




From: South Bay Technology :      Henriks-at-CompuServe.COM
Date: Wed, 17 Sep 1997 14:08:51 -0400
Subject: EM Lab Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am posting this for a friend who is not on the listserver. Please reply=

directly to Trinity College.

Thank you!

David Henriks
South Bay Technology, Inc.



************************ PLEASE POST ************************


Department of Human Resources
Trinity College
Hartford, Connecticut 06106

Position Announcement

Electron Microscopy Laboratory Manager/ Technician

Trinity College has an immediate opening for an experienced electron
microscopist to work in a newly established Electron Microscopy Center. A=
s
this facility is designed to serve both the physical and biological
sciences, familiarity with both disciplines is highly preferred. Primary
responsibilities include assisting faculty and students in the use of the=

facility for teaching and research, and overseeing the upkeep and use of
TEM, STEM and specimen preparation facilities, including the analytical E=
M
EDX and EELS). A B.S. degree with advanced research training and/or
extensive experience with TEM are essential; master's degree with requisi=
te
experience is preferred.

This position is a full-time, 12-month appointment with full College
benefits. Applications will be reviewed upon receipt; search will continu=
e
until position is filled. Please respond with a resume, cover
letter stating salary expectatons, and the names, telephone numbers and
addresses of three professional references to: Prof. Daniel Blackburn, c=
/o
Human Resources, 300 Summit Street, Trinity College, Hartford, CT 06106.=

(Resumes also may be faxed: (860) 297-5140, or sent via the internet:
Sandra.Magee-at-Mail.Trincoll.edu).

Trinity College is an Equal Opportunity/Affirmative Action Employer. Wome=
n
and mminorities are encouraged to apply. Applicants with disabilities
should request any needed accommodation in order to participate in the
application process.




From: José Luis Encinas :      encina1-at-ibm.net
Date: Wed, 17 Sep 1997 21:27:37 -0700
Subject: Subscribe Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Subscribe Microscopy encina1-at-ibm.net




From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 17 Sep 1997 15:48:54 -0400
Subject: RE: Pirani Gage

Contents Retrieved from Microscopy Listserver Archives
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The Duniway Stockroom Corp. ((800-446-8811) deals in new, used, and rebuilt
vacuum devices, including all types of vacuum gauges. They might be able
to help you with a replacement gauge, or possibly they could rebuild yours.

Good luck,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:313-763-4788; Ph:313-764-3321






From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 17 Sep 1997 15:56:37 -0400
Subject: Where to get Torr Seal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have received a couple of inquiries about what Torr Seal is and where to
get it.

Torr Seasl is an epoxy compound especially formulated for use in vacuum
systems that was introduced by Varian Associates, Vacuum Products Division,
121 Hartwell Ave, Lexington, MA 02173-3133 (800-882-7426) a number of years
ago. It is stated to contain no solvents, and therefore to be good at
pressures below 10-9Torr. It is bakeable at temperarures up to 120C. It
adheres to most clean materials (glass, metals, ceramics), and holds up
well over long term service. Some companies that handle EM supplies also
handle it (e.g. I find it listed in the Ladd catalog, probably SPI handles
it too).

Other similar products are also sold by other companies. For example,
Duniway Stockroom Corp. (800-446-8811; info-at-duniway.com) sells a product
called 'Epoxy Patch' that is stated to be equivalent to Torr seal

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:313-763-4788; Ph:313-764-3321






From: Yifan Cheng :      ycheng-at-zen.sb.fsu.edu
Date: Wed, 17 Sep 1997 17:30:30 -0400
Subject: Re: screen size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,
I am forwarding this message from SAFETY listserver.
Marek Malecki.


On Sep 17, 8:56am, Dr. Gary Gang REN wrote:
} Subject: screen size
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} } Did anybody know the size of large and small viewing screen of CM120
} } and CM200/FEG. Your information will be greatly appreciated!
} } gary
} }
} }
} } +--------------------------------------------------------------------+
} } | Gary Gang REN, Ph.D. |
} } | Department of Cell Biology #MB219 Email: gangren-at-scripps.edu |
} } | Mail Stop MB21 Tel: (619) 784 9815 (O) |
} } | The Scripps Research Institute (619) 546 1585 (H) |
} } | 10550 N. Torrey Pines Road Fax: (619) 784 9927 |
} } | La Jolla, CA 92037, USA http://leonardo.scripps.edu/ren |
} } +--------------------------------------------------------------------+
} }
} }
} }
}
}
} -- End of excerpt from Dr. Gary Gang REN


You can measure it quite accurately by using the measuring function of the CM
scope. Just move any thing you can identify from one side of the screen to the
other side of the screen.

Yifan Cheng

--
**********************************************************************
* Dr. Yifan Cheng * Phone: +1-850-644-4104 (Office)*
* Institute of Molecular Biophysics * or: +1-850-644-9769 (Lab) *
* Florida State University * Fax: +1-850-561-1406 *
* Tallahassee, FL 32306-4380 * Email: ycheng-at-sb.fsu.edu *
* U.S.A. * http://www.sb.fsu.edu/~ycheng *
**********************************************************************




From: Marek Malecki, M.D., Ph.D. :      malecki-at-MACC.WISC.EDU
Date: Wed, 17 Sep 1997 20:54:59 -0700
Subject: Cryo-stage for light microscopy.

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,
To evaluate effects of various cryoprotectants on the cells' viability, we
would like to image cultured cells being frozen. Is anybody aware of a
cryo-stage (below -100deg.C) for light microscopy?
Any information will be greatly appreciated.
Vendors welcome.
Sincerely,
Marek Malecki.







From: Ron Kalil :      rekalil-at-facstaff.wisc.edu
Date: Wed, 17 Sep 1997 21:15:31 -0500
Subject: New Diamond Knife For Sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FOR SALE: Brand New 6.2 mm Dupont diamond knife. Never used. Current retail
for a knife of this length is approximately $6000+. I would like to sell
the knife for $3200 or best offer.
Ron Kalil


Ronald Kalil
Center for Neuroscience
University of Wisconsin
1300 University Ave.
Madison, WI 53706

TEL: 608-262-4903
FAX: 608-265-2267
Email: rekalil-at-facstaff.wisc.edu
URL: http://www.neuroscience.wisc.edu





From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 17 Sep 97 23:09:59 -0500
Subject: Sample markings

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Has anyone ever heard of something called the "Arkograf electrical metal
etching pen?" Or more importantly, the name of the manufacturer and where
they are located? It is a device for making permanent markings on metal
surfaces for identification purposes.

Thanks.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================








From: Melvyn Dickson :      M.Dickson-at-unsw.edu.au
Date: Thu, 18 Sep 1997 14:56:29 +1000
Subject: EDM spark erosion cutter. Need circuits

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello World,

We have an Electric Discharge Machine Mark III, Ser 338854; It came
originally from Concept EDM Ltd, Maidenhead, Berkshire England. I checked
the UK yellow pages but they seem no longer to exist.


Our problem is that the Electric Discharge Machine won't work and we would
like the circuit diagrams (schematics) before trying to fix it.

If anyone out there has knowledge of the current address etc. of the
original suppliers, OR has a circuit diagram they could copy for us, we
would be most grateful.

Thanks,



Mel Dickson
President, Australian Society for Electron Microscopy
Director, Electron Microscope Unit,
University of New South Wales.
Sydney NSW 2052 Australia

Phone (+612) 9385-6383
Fax (+612) 9385-6400

Website {http://emunit1.babs.unsw.edu.au/emu_top.htm}




From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Thu, 18 Sep 1997 16:00:06 +0900
Subject: Re: Vignetting?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My belated thanks for responding to my query into "vignetting" I posted on the Microscopy list. Your comment was very helpful and enable me to get the job done.

Thank you very much again.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451






From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Thu, 18 Sep 1997 15:59:51 +0900
Subject: RE: Vignetting?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My belated thanks for responding to my query into "vignetting" I posted on the Microscopy list. Your comment was very helpful and enable me to get the job done.

Thank you very much again.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451






From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Thu, 18 Sep 1997 15:59:32 +0900
Subject: Re: Vignetting?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My belated thanks for responding to my query into "vignetting" I posted on the Microscopy list. Your comment was very helpful and enable me to get the job done.

Thank you very much again.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451






From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Thu, 18 Sep 1997 16:00:20 +0900
Subject: RE: Vignetting?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My belated thanks for responding to my query into "vignetting" I posted on the Microscopy list. Your comment was very helpful and enable me to get the job done.

Thank you very much again.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 18 Sep 1997 07:56:59 +0100
Subject: Re: Mercury Poisoning Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Dear Microscopists,
} I am forwarding this message from SAFETY listserver.
} Marek Malecki.
}
} Date: Wed, 17 Sep 1997 13:25:29 -0400
} From: "Ralph Stuart, Vermont SIRI" {rstuart-at-esf.uvm.edu}
}
} A Tiny Drop Of Mercury Shatters Lives And Science
}
} A9 1997 The Associated Press
}
} LYME, N.H. (September 13, 1997 6:45 p.m. EDT) -
}
snips

} "She loved her work," he says. "It made her happy."
}
} She couldn't have known the risks. She couldn't have known how bad the bad
} stuff really was. Truth is, no one knew.
}
} Just a tiny drop of liquid. Sweet-smelling. Dense. Deadly.
}
} By HELEN O'NEILL, The Associated Press

While not wishing to diminish the dangers of mercury, I really don't see
that this sort of journalistic hype helps anybody. It makes a good story, I
suppose, for the sort of newspaper that runs 'Elvis Lives' stories, but it
isn't science and even the courts regard hearsay as highly unreliable.

Regards,

Larry Stoter






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 18 Sep 1997 07:56:59 +0100
Subject: Re: Mercury Poisoning Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Dear Microscopists,
} I am forwarding this message from SAFETY listserver.
} Marek Malecki.
}
} Date: Wed, 17 Sep 1997 13:25:29 -0400
} From: "Ralph Stuart, Vermont SIRI" {rstuart-at-esf.uvm.edu}
}
} A Tiny Drop Of Mercury Shatters Lives And Science
}
} A9 1997 The Associated Press
}
} LYME, N.H. (September 13, 1997 6:45 p.m. EDT) -
}
snips

} "She loved her work," he says. "It made her happy."
}
} She couldn't have known the risks. She couldn't have known how bad the bad
} stuff really was. Truth is, no one knew.
}
} Just a tiny drop of liquid. Sweet-smelling. Dense. Deadly.
}
} By HELEN O'NEILL, The Associated Press

While not wishing to diminish the dangers of mercury, I really don't see
that this sort of journalistic hype helps anybody. It makes a good story, I
suppose, for the sort of newspaper that runs 'Elvis Lives' stories, but it
isn't science and even the courts regard hearsay as highly unreliable.

Regards,

Larry Stoter






From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Thu, 18 Sep 1997 17:25:31 +0900
Subject: Sorry!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Terribly embarrassed... Please accept my apology for the 5 or 6 private thank-yous I just posted. I just kept hitting reply without realizing it was a mailing list.


Chiba Atsushi [(Mr.) -- *Chiba* is my surname]
Voice: (+81) 010-045-9451






From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 18 Sep 1997 09:43:17 +0000
Subject: Mercury Poisoning Story -Reply

Contents Retrieved from Microscopy Listserver Archives
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The story can be checked out at the following web site:

http://vest.gu.se/~bosse/Mercury/Mail/Msg.0005.html

Regards - Keith Ryan
Plymouth Marine Lab., UK




From: rschoonh-at-sph.unc.edu (Robert Schoonhoven)
Date: Thu, 18 Sep 1997 08:27:43 +0000 (est)
Subject: Re: Oil Red O for GMA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The procedure that Steven posted does indeed work very nicly.....Provided that no alcohol was used
in the "dehydration" process. If you wish to look for lipids in GMA embedded tissues you must use
a series of graded (with water) monomer solutions instead of alcohols. Somewhere ????? I have the
procedure written but I can't put my hands on it right now (ie: I haven't got a clue as to where I
'filed' it). :(

regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123





From: C. John Runions :      cjr14-at-cornell.edu
Date: Thu, 18 Sep 1997 08:48:39 -0400
Subject: Re: Mercury Poisoning Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My thoughts exactly, see the August? issue of Scientific American for a
better tribute to Karen Wetterhahn and a less subjective discussion of the
risks associated with chemical handling.


}
} While not wishing to diminish the dangers of mercury, I really don't see
} that this sort of journalistic hype helps anybody. It makes a good story, I
} suppose, for the sort of newspaper that runs 'Elvis Lives' stories, but it
} isn't science and even the courts regard hearsay as highly unreliable.
}
} Regards,
}
} Larry Stoter



=================
C. John Runions
Section of Ecology and Systematics
Corson Hall
Cornell University
Ithaca, New York
USA 14853

email cjr14-at-cornell.edu
phone (607) 254-4282
Fax (607) 255-8088






From: Evex Analytical :      Evex_Analytical-at-evex.com
Date: Thu, 18 Sep 1997 09:01:37 -0400
Subject: X-ray Analyzer wish list. Build it the way you want it.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greeting fellow microsopist,

Evex Analytical, manufacture of the VIDX X-ray Analyzer and the VIDX =
Scan Digital Imaging system for SEM/STEM and TEM, would like your feed =
back.

We are now engineering Version 3.0 of our VIDX X-ray analyzer software. =
We would like to ask you for your wish list. Your wish list may include =
functions, routines, macros, etc. which you would like to see =
implemented in your ideal x-ray analyzer, This inquiry is open to every =
one.

Please respond via e-mail. You may foward drawing also.


Thank you


Peter Tarquinio
Evex Analytical
www.evex.com
609-252-9192 Tel
609-252-9091 Fax




From: Jeanne Barker :      jeanne_barker-at-merck.com
Date: Thu, 18 Sep 1997 08:56:07 -0400
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

9/18/97 8:49 AM
Subscribe

Subscribe please
Thanks, Jeanne





From: Joseph P. Neilly 847-938-5024 :      NEILLY.JOSEPH-at-igate.abbott.com
Date: Thu, 18 Sep 1997 09:14:00 -0500 (CDT)
Subject: RE: Cryo-stage for light microscopy.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marek,

Linkam make a very nice heating/cooling stage for light micrscopes with
a temperature range of -196 to 600 C. They are distributed locally by
Fryer Co. Phone 847-669-2000.

Regards,

Joe Neilly
Abbott Laboratories
Microscopy and Microanalysis
200 Abbott Park Rd.
Abbott Park, IL 60064





From: Ann-Fook Yang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Thu, 18 Sep 1997 10:35:48 -0400
Subject: request for e-mail address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am posting this message for a friend who would
like to have the e-mail address of Bill McManus,
Utah State Univ., Dept . Biology, Logan, Utah.
Please reply to :

Kalabm-at-em.agr.ca

Ann Fook Yang
EM Unit,
ECORC,
Agriculture and Agri-Food Canada,
Central Experimental Farm,
Ottawa, Ontario,
Canada K1A 0C6

Tel:+1-613-759-1638
Fax: +1-613-759-1701
e-mail: yanga-at-em.agr.ca




From: Rik Brydson :      MTLRMDB-at-LUCS-MAC.NOVELL.LEEDS.AC.UK
Date: Thu, 18 Sep 1997 14:48:59 GMT0BST
Subject: Interfaces meeting in UK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

If anyone is over in UK at the end of February please bear in mind the fol=
lowing meeting....


*************************************************************
Microscopy of Internal Interfaces: Determination of=
Nanochemistry

Institute of Physic=
s,
76 Portland Place, London W1N 4AA,=
UK.

Friday 27 February 1998=

10.00am - 5.30pm

Organizer: Dr Rik Brydson, School of Process, Environmental and Materials =
Engineering,
University of Leeds, Leeds LS2 9JT, UK.

EMAG Committee of Institute of Physics
Joint with Royal Microscopical Society: Materials Section.

The chemistry of internal interfaces in both structural and functional mat=
erials is often a
critical factor affecting resultant physical properties, sometimes overrid=
ing or controlling the
effects of interface structure. The majority of interfaces have a spatial =
extent, in at least one
dimension, of a few nanometres. Clearly it is desirable to be able to dete=
rmine accurately
interfacial chemistry and bonding. With this in mind, a one day IOP meetin=
g will be held in
London at the end of February 1998 on the techniques for interfacial micro=
analysis and
relevant case studies in this rapidly expanding field. The day will be div=
ided into fundmentals
and applications sessions.

Confirmed Invited Speakers:
oProf. Mick Brown (Cambridge) "Grain boundary chemistry in metals and all=
oys"
(Sponsored by RMS).
o Dr Rik Brydson (Leeds) "Interfacial bonding determined by EELS"
o Dr David Jefferson (Cambridge) "Determination of surface chemistry using=
HREM"
o Prof. John Titchmarsh (Sheffield Hallam) "Determining Interfacial Segreg=
ation using
EDX"
o Dr John Watts (Surrey) "Surface Analysis applied to Interfaces"
o Prof Bruce Hamilton/ Dr Uschi Bangert (UMIST) "STM/STEM of cleaved semic=
onductor
multilayers"
o Dr Paul J Warren (Oxford) "Atom Probe Field Ion Microscopy of Internal I=
nterfaces".

Other topics will include:
o Interfacial Chemistry and HREM
o STM on cross-sections
o Theoretical aspects of interface chemistry
o Metal-support interactions in Supported Catalysts
o Semiconductor interfaces
o Biomaterial interfaces
o Coatings
o Corrosion Films

Additional POSTER contributions are extremely welcome.

For further details about registration etc. please contact
IOP conference desk Tel: 0171 470 4800 Fax: 0171 470 4848 Email: physics-at-i=
op.org
or
Rik Brydson Tel: 0113 233 2369 Fax: 0113 242 2531 Email: mtlrmdb-at-leeds.ac=
.uk
_____________________________
Dr. Rik Brydson,
University Research Fellow,
Electron Optical Unit,
Department of Materials,
School of Process, Environmental and Materials Engineering
University of Leeds,
Leeds LS2 9JT,
U.K.

Tel: 44 + (0)113 233 2369
Fax: 44 + (0)113 242 2531
_____________________________





From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Thu, 18 Sep 1997 10:45:16 -0400
Subject: LM: Fluorescent Bulb Replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone know of a source for the HBO 100W/2 100 watt bulbs
used for fluorescence light microscopy?

Thanks,

Dennis Shubitowski
University of Michigan
School of Dentistry
dshubito-at-umich.edu






From: Katherine.S.Connolly-at-dartmouth.edu (Katherine S. Connolly)
Date: 18 Sep 97 10:54:13 EDT
Subject: Mercury Poisoning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

As part of the group involved in the postmortem examination of the death of
Karen Wetterhahn I was grateful for all the information and warnings I could
lay my hands on before I dealt with any material. The event ,however reported,
serves as a reminder to all of us of the dangers of our materials and the
need to keep up with the information involved in our safety.
This institution has completely reexamined it's glove policy.

Kate Connolly




From: MIKE ROCK :      merock-at-du.edu
Date: Thu, 18 Sep 1997 10:02:58 -0600 (MDT)
Subject: Re: Mercury Poisoning Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey Larry-
how many electron microscopists have you met who's hand trembles and
shakes as they reach out to shake your hand, or dumps the food from
their fork or plate as they struggle to eat lunch?
I believe they, their family and friends don't look at this as trash
journalism, we should all remember SAFETY FIRST.
-Mike Rock

On Thu, 18 Sep 1997, Larry
Stoter wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} } Dear Microscopists,
} } I am forwarding this message from SAFETY listserver.
} } Marek Malecki.
} }
} } Date: Wed, 17 Sep 1997 13:25:29 -0400
} } From: "Ralph Stuart, Vermont SIRI" {rstuart-at-esf.uvm.edu}
} }
} } A Tiny Drop Of Mercury Shatters Lives And Science
} }
} } A9 1997 The Associated Press
} }
} } LYME, N.H. (September 13, 1997 6:45 p.m. EDT) -
} }
} snips
}
} } "She loved her work," he says. "It made her happy."
} }
} } She couldn't have known the risks. She couldn't have known how bad the bad
} } stuff really was. Truth is, no one knew.
} }
} } Just a tiny drop of liquid. Sweet-smelling. Dense. Deadly.
} }
} } By HELEN O'NEILL, The Associated Press
}
} While not wishing to diminish the dangers of mercury, I really don't see
} that this sort of journalistic hype helps anybody. It makes a good story, I
} suppose, for the sort of newspaper that runs 'Elvis Lives' stories, but it
} isn't science and even the courts regard hearsay as highly unreliable.
}
} Regards,
}
} Larry Stoter
}
}
}




From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Thu, 18 Sep 1997 11:24:26 -0500
Subject: Re: LM: Fluorescent Bulb Replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have recently bought several different types of bulbs from Lamp
Technology (Bohemia, NY) at 800-533-7548. There prices are a lot better
that anything I have ever found through a microscope dealer. One of my
dealers told me that I was getting a better price than he was wholesale.
I recommend you give them a call. They have a web page but I can't
remember the address - use a search engine if you are interested. I have
no interest in the company except as a very happy user.

} Does anyone know of a source for the HBO 100W/2 100 watt bulbs
} used for fluorescence light microscopy?
}
} Thanks,
}
} Dennis Shubitowski
} University of Michigan
} School of Dentistry
} dshubito-at-umich.edu


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: ejb11-at-psu.edu (Edward J. Basgall)
Date: Thu, 18 Sep 1997 15:00:30 -0500
Subject: Re: LM: Fluorescent Bulb Replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dennis,
Try

PSC Lamps, Inc., 1 Fishers Rd., Pittsford, NY 14534
1-800-772-5267 FAX 1-800-257-0760
http://www.roccplex.com/psclamps

They were rated a favorite last year by Beth Richardson (UGA) who was on a
similar quest.

(No affiliation blah, blah, blah.....)

cheers
ed

Edward J. Basgall, PhD
The Pennsylvania State University
Surface Chemistry Group ejb11-at-psu.edu
Materials Research Institute Building Ph: 814-865-0493
University Park, PA 16802-7003 FAX: 814-863-0618
http://www.personal.psu.edu/ejb11/






From: David A. Stanford :      DSTANFORD-at-CompuServe.COM
Date: Thu, 18 Sep 1997 15:02:14 -0400
Subject: Web page announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello to All

Advanced MicroBeam, Inc. would like to announce its new web presence to t=
he
microscopy community. We can be found at http://www.advancedmicrobeam.co=
m.
Please take a look at how we may be of service to you.

Thanks Dave

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D
David A. Stanford
Advanced MicroBeam, Inc.
4217C King Graves Rd
PO Box 610
Vienna OH 44473-0610

Phone: 330-394-1255
Fax: 330-394-1834
Email: dstanford-at-advancedmicrobeam.com

Web: www.advancedmicrobeam.com
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D




From: Patrick Huddie :      phuddie-at-microcosm.com
Date: Thu, 18 Sep 1997 16:12:39 -0400
Subject: Re: LM: Fluorescent Bulb Replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dennis Shubitowski wrote:

} Does anyone know of a source for the HBO 100W/2 100 watt bulbs used
} for fluorescence light microscopy?

Yes

Either:

Bulbtronics, Inc., 45 Banfi Plaza, Box 306, Farmingdale, NY 11735
Phone: 800-654-8542: Fax: 516-249-6066

Or:
Microcosm [ :-) ] - how many do you need?


--------------------------------------------------------------
Dr. Patrick L. Huddie (301) 725-2775 Fax (301) 725-2941
Microcosm, Inc., 9140 Guilford Road, Suite O, Columbia, MD 21046
e-mail phuddie-at-microcosm.com URL http://www.microcosm.com
The Web is:"Vaster than empires and more slow" Andrew Marvell
(1621-1678)






From: rick-at-pgt.com (Rick Mott)
Date: Thu, 18 Sep 97 16:39:46 EDT
Subject: Re: Mercury Poisoning Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Without in any way downplaying the tragedy for the people involved, the
dose-response relationship postulated in the story is not very plausible.
Mercury toxicity has been studied extensively for at least half a century.
It is a cumulative poison which does cause the type of neurological effects
reported, but a "single tiny drop", through a glove and quickly washed off,
being lethal seems suspect. Associated Press is not peer reviewed (although
maybe it depends on your definition of "peer").

In 1996, the EPA published a 7-volume report on environmental mercury.
For some data from recent animal studies (rodent and primate) and
the current EPA and FDA reference exposure limits, try the following
Web pages.

ehpnet1.niehs.nih.gov/docs/1996/Suppl(2)/rice1.html (70kb)

www.me3.org/projects/costs/hgrefdose.html ( 3kb)

I think it is a disservice to the public to exaggerate risks beyond
reason, just as it is wrong to gloss over them.

Rick Mott
rick-at-pgt.com

(These are personal views having nothing to do with my employer)




From: South Bay Technology :      Henriks-at-CompuServe.COM
Date: Thu, 18 Sep 1997 16:42:53 -0400
Subject: resolution test at a tilt angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Cheng:

An excellent sample to test resolution at high tilt angles is a single
crystal silicon wafer. If mounted as a cross-sectional sample (electron
beam parallel to a {011} zone axis), there are several advantages. In th=
is
initial orientation, the (111) lattice spacings of 3.14 A, (200) spacings=

of 2.715A and (220) spacings of 1.92A are all available for resolution
tests. Being a cubic single crystal, it is a simple procedure to follow t=
he
Kikuchi bands to any other cubic zone axis for the additional resolution
checks you mentioned.The resolution at high tilt angles can be checked by=

mounting the cross-sectional silicon sample with the sample's glue line
either parallel or perpendicular to the sample holder rod. With the glue=

line parallel to the sample holder rod, {100} zone axes are accessible by=

rotating the sample holder 45 degrees in either direction. The {100} zone=

includes (220) spacings (200) spacings for resolution tests. Similarly, =
if
the sample is mounted with the glue line perpendicular to the sample hold=
er
rod, the second tilt attachment can be used to tilt the sample 45 degrees=

to the {100} zone axis in either direction about the second tilt axis. =
If
your goniometer has a limited tilt capability, there are many other choce=
s
of zones at lower (and higher) angles for you to choose from.

While you are performing these resolution tests, there are several other
calibrations that are easy to perform and can be done with the same sampl=
e.
For example, the complete magnification range of the TEM (the supplied
values from the manufacturer can be 5-10% off), the camera constant
calibration, and the imge/diffraction patter rotation calibration. All o=
f
these tests and calibrations are extensively documented for the MAG*I*CAL=

calibration sample, which is a single crystal of silicon upon which are
precisely grown calibration marks. =


PLEASE NOTE: South Bay Technology, Inc. does offer the MAG*I*CAL
Calibration Standard and so we have a finiancial interest in promoting it=
s
use. For more inforamtion on the MAG*I*CAL, please contact me off-line.

Best regards-

David

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++=
++
++

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
=

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++=
++
++

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.
I have a couple of questions and will appreciate any replies and =

suggestions.

1) What is the best sample to test the resolution of a TEM at a tilt
angle
(} 30degree)?

I am testing the resolution of a CM300FEG microscope. I used oriented
gold,
evaperated gold particles, graphitized carbon and so on. There is no =

problem of
those samples at zero tilt. But after tilt the specimen to 30 degree or =

higher,
I got only the good resolution (2.3A in case of evaperated gold
particle) =

in
one direction, along the tilt axis. I believed it is not because of the =

focus
gradient that I could not get the same resolution in both directions at =

this
tilt angle. I think the samples (evaporated gold particles and
graphitized
carbon) may not be the suitable samples for the resolution test of the
high
tilt angles (higher than 45 degree and up to 60 degree). I wonder if any
of
you
has done such test before and has any suggestions on which specimen
should =

be
used in this test and where to get it?

2) Is there any published documentation about the line resolution of
Kodak
SO-163 film?

I am writing an paper about the performance of the CM300FEG at low
magnificaiton, and need to know the line resolution of Kodak SO-163 =

(something
I can cite for). But I could not find any published documentation. Of =

course, I
called Kodak technique support and of course they didn't give me a clue.
There
is a web site called "Bibliography on EM Imaging and Related
Technologies"
(http://www.uct.ac.za/depts/emu/imaging/papers.htm), but I can not find
any
thing there either about SO-163. The only other related information I
got =

was
from a similar discussion in this board a year ago. But the data about
line
resolution found in that discussion were mostly estimated but not from
any
published documentation. I wonder if any of you know there is any kind
of
published documentation including research paper, technique report or so
which
gives the line resolution of Kodak SO-163. (BTW, the topic about the
line
resolution has been discussed a year ago and I am sorry to ask it
again.)

I appreciate any information or suggestion on my request.

Yifan Cheng

BTW, I am not sure if my email address is already on the list or not. So
that I
appreciate also that when you reply this email, send a reply to me
directly
as
well as to the board. Thanks again.


--
**********************************************************************
* Dr. Yifan Cheng * Phone: +1-850-644-4104 (Office)*
* Institute of Molecular Biophysics * or: +1-850-644-9769 (Lab) *
* Florida State University * Fax: +1-850-561-1406 *
* Tallahassee, FL 32306-4380 * Email: ycheng-at-sb.fsu.edu *
* U.S.A. * http://www.sb.fsu.edu/~ycheng *
********************************************************************** =





From: zhang-at-cvlab.harvard.edu (Dorothy Zhang)
Date: Thu, 18 Sep 1997 15:42:28 -0500
Subject: Insitu and IHC double staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear reader,
I tried to detect mRNA and protien in the same slide. My insitu
hybridyzation and immunohistochemistry both worked. but not working when I
put them together. I tried insitu first then IHC. Is there anyone tell me
some tricks about it? Thanks.
Dorothy






From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Thu, 18 Sep 1997 16:57:36 -0400
Subject: Re; Fluorescent Bulb Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As usual the listserver has been exceedingly helpful. Thank
you very much for all the replies.

Dennis Shubitowski
University of Michigan
School of Dentistry
dshubito-at-umich.edu






From: vhacopian-at-wellesley.edu (Vachik Hacopian)
Date: Thu, 18 Sep 1997 18:03:52 -0500
Subject: Re: Mercury Poisoning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kate, would you share with us the outcome of your institution's
re-examination of its glove policy? Thanks. /Vachik Hacopian



} As part of the group involved in the postmortem examination of the death of
} Karen Wetterhahn I was grateful for all the information and warnings I could
} lay my hands on before I dealt with any material. The event ,however reported,
} serves as a reminder to all of us of the dangers of our materials and the
} need to keep up with the information involved in our safety.
} This institution has completely reexamined it's glove policy.
}
} Kate Connolly






From: Andrea Therese Hooper :      hoopea01-at-mchip00.med.nyu.edu
Date: Thu, 18 Sep 97 18:23:05 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe "hoopea01-at-popmail.med.nyu.edu"






From: Marek Malecki :      malecki-at-MACC.WISC.EDU
Date: Thu, 18 Sep 1997 18:17:48 -0700
Subject: Re: Insitu and IHC double staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dorothy,
You may want to visit the WWW site by Dr. Gwen Childs. It contains the
detailed hands on protocols whuch you need and the excellent examples of
labelings:ISH and IL.
http://cellbio.utmb.edu/childs/childs.htm

Sincerely,
Marek Malecki.


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America







From: Christine C. Broadbridge
Date: Thu, 18 Sep 1997 19:34:51 -0500
Subject: Position Announcement for Microscopy Lab Manager

Contents Retrieved from Microscopy Listserver Archives
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************************ PLEASE POST ************************


Department of Human Resources
Trinity College
Hartford, Connecticut 06106

Position Announcement

Electron Microscopy Laboratory Manager/ Technician

Trinity College has an immediate opening for an experienced electron
microscopist to work in a newly established Electron Microscopy Center. As
this facility is designed to serve both the physical and biological
sciences, familiarity with both disciplines is highly preferred. Primary
responsibilities include assisting faculty and students in the use of the
facility for teaching and research, and overseeing the upkeep and use of
TEM, STEM and specimen preparation facilities, including the analytical EM
EDX and EELS). A B.S. degree with advanced research training and/or
extensive experience with TEM are essential; master's degree with requisite
experience is preferred.

This position is a full-time, 12-month appointment with full College
benefits. Applications will be reviewed upon receipt; search will continue
until position is filled. Please respond with a resume, cover
letter stating salary expectatons, and the names, telephone numbers and
addresses of three professional references to: Prof. Daniel Blackburn, c/o
Human Resources, 300 Summit Street, Trinity College, Hartford, CT 06106.
(Resumes also may be faxed: (860) 297-5140, or sent via the internet:
Sandra.Magee-at-Mail.Trincoll.edu).

Trinity College is an Equal Opportunity/Affirmative Action Employer. Women
and minorities are encouraged to apply. Applicants with disabilities
should request any needed accommodation in order to participate in the
application process.



*****************

Christine Caragianis Broadbridge, Assi. Prof.
Trinity College, Department of Engineering
300 Summit Steet
Hartford, CT 06106-3100

Office Tel: (860) 297-5343
Lab Tel: (860) 297-5202
Fax: (860) 297-3531

christine.broadbridge-at-mail.trincoll.edu






From: fischg98-at-providence.edu (by way of Nestor J. Zaluzec)
Date: Thu, 18 Sep 1997 21:22:00 -0500
Subject: Ask-A-Microscopist: Specimen Prep Leishmania enrietti

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Forwarded Email please reply direclty to fischg98-at-providence.edu


Below is the result of your feedback form. It was submitted by
(fischg98-at-providence.edu) on Tuesday, September 16, 1997 at 11:34:39
---------------------------------------------------------------------------

Email: fischg98-at-providence.edu
Name: Gia Fischetti

School: Providence College

State: RI

Zip: 02918

Question: I am currently doing research on Leishmania enrietti,
and I am now tring to prepare specimines for our
TEM. I have been unsuccessful at finding a "recipe"
for the preparation of this organism. I am reluctant
to just try Karnovsky's fixative, and would appreciate
any help that you could give me on this matter.
Thank you very much. I hope to hear from you soon.
Gia Fischetti

---------------------------------------------------------------------------






From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 19 Sep 1997 08:23:59 +0000
Subject: Corrected Mercury link

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Apologies to those who have contacted me so far, the correct address
is:

http://vest.gu.se/~bosse/Mercury/Mail/Msg0005.html

If you read the obituary etc., she was clearly quite a scientist. A moral
here is check that your gloves are good for what you are using, we
have toxicologists/occasional electron microscopists using tributyl tin
which is also not nice.

Keith Ryan
Plymouth Marine Lab., UK





From: Correo Xeral :      correo-at-cogami.es
Date: Fri, 19 Sep 1997 11:14:07 +-200
Subject: Remove me

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Sirs: please remove my name from your list.





From: rschoonh-at-sph.unc.edu (Robert Schoonhoven)
Date: Fri, 19 Sep 1997 07:30:35 +0000 (est)
Subject: Re: Mercury Poisoning Story

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Rick,
While the AP is not peer reviewed Science is. Suggest that you check out the August issue. Karen
was a collaberator with several people in our lab. Also suggest that you look up
"dimethylmercury". One drop (50ul) is equal to about 300X the occupational exposure limit.
-- Begin original message --


}
}
} Without in any way downplaying the tragedy for the people involved, the
} dose-response relationship postulated in the story is not very plausible.
} Mercury toxicity has been studied extensively for at least half a century.
} It is a cumulative poison which does cause the type of neurological effects
} reported, but a "single tiny drop", through a glove and quickly washed off,
} being lethal seems suspect. Associated Press is not peer reviewed (although
} maybe it depends on your definition of "peer").
}

} Rick Mott
} rick-at-pgt.com
}
} (These are personal views having nothing to do with my employer)
}

-- End original message --


regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123





From: David L Johnson :      jptmvl-at-mailbox.syr.edu
Date: Fri, 19 Sep 1997 08:32:33 -0400 (EDT)
Subject: Help with bse imaging of diatoms...

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I'm doing automated particle analysis (SEM/EDX) of suspended particulate
material from drinking water reservoirs. Particles are filtered onto
nuclepore membranes and carbon coated. Features are located using a
thresholded bse image. Diatoms (some species) are so thin that that they
don't image very well. Does anybody know of a 'staining' method to
increase the atomic number contrast for amorphous silica?

reply to jptmvl-at-mailbox.syr.edu thanx, dave johnson






From: paqui-at-iris1.fae.ub.es
Date: Fri, 19 Sep 1997 14:58:07 +0000
Subject: QUANTITEM

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Dear Colleagues,

I would appreciate any information about the software QUANTITEM for
quantitative determination of interfacial roughness and composition
mappings in HREM images.
Thanks in advance.

F. Peiro
*******************************+
Francesca Peiro

EME, Enginyeria i Materials Electronics
Dpt. Fisica Aplicada i Electronica
Universitat de Barcelona
Avda. Diagonal 645-647
08028 Barcelona, Spain

Tel. (34-3) 402 11 39
Fax. (34 3) 402 11 48
e-mail: paqui-at-iris1.fae.ub.es
****************************





From: Vincent Chieh-Wen Tsai :      chieh-at-email.nist.gov
Date: Fri, 19 Sep 1997 09:28:13 -0400
Subject: unsubscribe

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unsubscribe "chieh-at-mailserver.nist.gov"





From: PLDahl-at-aol.com
Date: Fri, 19 Sep 1997 09:37:17 -0400 (EDT)
Subject: Mercury Poisoning

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The story of the death of Dartmouth College chemistry professor Karen
Wetterhahn is unfortunately very true and not greatly exaggerated. She was
using very small amounts of dimethylmercury as an NMR standard. The amount
reported is "one to a few drops". This is not a large exposure and the
exposure occured only once. She was reported to be a very careful chemist.
The latex gloves she was wearing did not stop the absorption of the
dimethylmercury through her skin. Treatment for mercury poisoning was
unsuccessful in saving her life.

Everyone needs to minimize their exposure to toxic materials and
microscopists are well aware of this as is noted in this listserver. The
composition of gloves worn in the laboratory is very important and each
application should be evaluated according to need. It serves no purpose to
downplay the potential for tragedy in the lab from toxic materials. Remember
also that the greatest potential for problems is the long term exposure to
small amounts of materials that the lab person has become accustomed to using
- familiarity breeds contempt. Two good articles about Karen Wetterhahn
appear in:
Chemical and Engineering News, June 16, 1997, p. 11, and
Scientific American, September, 1997, p. 20.


Phil Dahlstrom




From: Katherine.S.Connolly-at-dartmouth.edu (Katherine S. Connolly)
Date: 19 Sep 97 09:49:42 EDT
Subject: Mercury Poisoning/gloves

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The Dartmouth College Biosafety Office in combination with OSHA has produced a
comprehensive study /report on the characteristics and abilities of available
gloves. On a smaller scale , anyday, I expect a poster for all the labs
summarizing the chief points of this work.
Anyone wishing information on gloves should contact Michael Blayney at:
Michael.Blayney-at-dartmouth.edu

Kate Connolly




From: Jim Darley :      jim-at-proscitech.com.au
Date: Fri, 19 Sep 1997 23:58:14 +1000
Subject: Re: Mercury Poisoning

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Message-Id: {199709191355.XAA12669-at-ultra.ultra.net.au}

I cannot believe this discussion. No doubt mercury is a nasty cumulative
toxin. But blaming a faulty glove and a single drop passing onto the skin
is pure
nonsense.
In the good ol days I cleaned a number of times mercury diffusion pumps and
purified the mercury by shaking the stuff with nitric acid and solvents in
a separating funnel.
No gloves! I have no trace of Minnemata disease, steadier hands than most
people
and I am "approximately normal".
I knew a man who had active mercury poisoning. This was contracted in a
large
but closed room after a large industrial thermometer fractured on a very
hot oven. The man inhaled fumes for several hours.
Mercury poisoning most frequently is caused by ingestion and inhalation. I
do not advocate bathing in mercury, but that story I take with a drop of
something else!
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
Great microscopy catalogue, 400+ Links, MSDS
************************ http://www.proscitech.com.au
}
} Kate, would you share with us the outcome of your institution's
} re-examination of its glove policy? Thanks. /Vachik Hacopian
}
}
}
} } As part of the group involved in the postmortem examination of the
death of
} } Karen Wetterhahn I was grateful for all the information and warnings I
could
} } lay my hands on before I dealt with any material. The event ,however
reported,
} } serves as a reminder to all of us of the dangers of our materials
and the
} } need to keep up with the information involved in our safety.
} } This institution has completely reexamined it's glove policy.
} }
} } Kate Connolly





From: Scott Schwinge :      schwinge-at-fhl.washington.edu
Date: Fri, 19 Sep 1997 08:12:11 -0800
Subject: Re: Mercury Poisoning Story

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If I recall correctly, the compound in question was dimethyl mercury, which
turned out to pass quickly through her gloves, in contrast to metallic
mercury.

} Without in any way downplaying the tragedy for the people involved, the
} dose-response relationship postulated in the story is not very plausible.
} Mercury toxicity has been studied extensively for at least half a century.
} It is a cumulative poison which does cause the type of neurological effects
} reported, but a "single tiny drop", through a glove and quickly washed off,
} being lethal seems suspect. Associated Press is not peer reviewed (although
} maybe it depends on your definition of "peer").

Scott Schwinge
Friday Harbor Labs
University of Washington






From: Robert J. Palmer Jr. :      rjpalmer-at-utkux.utcc.utk.edu
Date: Fri, 19 Sep 1997 11:21:50 -0400 (EDT)
Subject: Hg, gloves, death

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Can we move the Hg discussion to www.Hgtox.com, the gloves discussion to
the handwear listserv, and get on to death of more important people (Di)?

Seriously, Prof. Wetterhahn and Di were interesting individuals who have
received their eulogies in other more appropriate forums. Whether one drop
of diMeHg causes death is debatable, but not here (are any of you
microscopists using it?). Gloves get holes and have unsealed wrists
(usually). Enough OK?
Rob Palmer
CEB/UT






From: Woody.N.White-at-mcdermott.com
Date: Fri, 19 Sep 1997 13:01:00 -0500
Subject: Re: Help with bse imaging of diatoms...

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If you can still excite the x-ray lines of interest, try lowering the
incident
beam potential. Woody
______________________________ Reply Separator
_________________________________

I'm doing automated particle analysis (SEM/EDX) of suspended particulate
material from drinking water reservoirs. Particles are filtered onto
nuclepore membranes and carbon coated. Features are located using a
thresholded bse image. Diatoms (some species) are so thin that that they
don't image very well. Does anybody know of a 'staining' method to
increase the atomic number contrast for amorphous silica?

reply to jptmvl-at-mailbox.syr.edu thanx, dave johnson




From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Fri, 19 Sep 1997 10:43:22 -0700
Subject: Re: LM: Fluorescent Bulb Replacement

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I get my Osram HBO 100W/2 for a price of $128.00 from

Elkay
P.O. Box 1105
La Jolla CA 92038-1105

Larry Kuritzky
619-454-5742


} Does anyone know of a source for the HBO 100W/2 100 watt bulbs
} used for fluorescence light microscopy?
}
} Thanks,
}
} Dennis Shubitowski
} University of Michigan
} School of Dentistry
} dshubito-at-umich.edu


---------------------------------------------------------------------

Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/EM_Facility






From: nancy buening :      buening-at-topaz.ucdavis.edu
Date: Fri, 19 Sep 1997 11:23:00 -0700
Subject: TEM Tripod polisher

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Would anyone know of a used tripod polisher I could buy? I need it to
prepare TEM sections of modern and fossil shells (brachiopods) made of
calcite.

Thanks.

Nancy Buening

Nancy Buening E-mail: buening-at-geology.ucdavis.edu
Department of Geology Phone: (916) 752-0350
University of California Fax: 916 752-0951
Davis, CA 95616






From: Bill Trevarrow :      trevarro-at-uoneuro.uoregon.edu
Date: Fri, 19 Sep 1997 11:29:30 -0700
Subject: Re: Mercury Poisoning

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I believe you have missed the point:
dimethyl mercury is different from the metallic mercury that you are
addressing.

} I cannot believe this discussion. No doubt mercury is a nasty cumulative
} toxin. But blaming a faulty glove and a single drop passing onto the skin
} is pure
} nonsense.
} In the good ol days I cleaned a number of times mercury diffusion pumps and
} purified the mercury by shaking the stuff with nitric acid and solvents in
} a separating funnel.
} No gloves! I have no trace of Minnemata disease, steadier hands than most
} people
} and I am "approximately normal".
} I knew a man who had active mercury poisoning. This was contracted in a
} large
} but closed room after a large industrial thermometer fractured on a very
} hot oven. The man inhaled fumes for several hours.
} Mercury poisoning most frequently is caused by ingestion and inhalation. I
} do not advocate bathing in mercury, but that story I take with a drop of
} something else!






From: Vladimir Dusevich :      dusevich-at-astro.ocis.temple.edu
Date: Fri, 19 Sep 1997 14:55:48 -0400 (EDT)
Subject: Re: Hg, gloves, death

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100% agree!!!

On Fri, 19 Sep 1997, Robert J. Palmer Jr. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Can we move the Hg discussion to www.Hgtox.com, the gloves discussion to
} the handwear listserv, and get on to death of more important people (Di)?
}
} Seriously, Prof. Wetterhahn and Di were interesting individuals who have
} received their eulogies in other more appropriate forums. Whether one drop
} of diMeHg causes death is debatable, but not here (are any of you
} microscopists using it?). Gloves get holes and have unsealed wrists
} (usually). Enough OK?
} Rob Palmer
} CEB/UT
}
}
}




From: Mark E. Darus (216) 266-2895 General Electric Co. :      darus-at-cle.dnet.ge.com
Date: Fri, 19 Sep 97 15:14:14 EDT
Subject: Re: Hg, gloves, death

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Lets throw some mercury on the mercury story.




From: Randy Tindall :      rtindell-at-nmsu.edu
Date: Fri, 19 Sep 1997 13:40:16 -0600
Subject: Re: BSE imaging of diatoms

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Dave,

If you have the option, I would try low kV secondary imaging to do your
image analysis, using kV's of 5-10, or even lower. Then later, go to
backscatter and increase your accelerating voltage for EDX.

The problem with stains that increase your atomic number contrast, it seems
to me, is that they would also greatly affect your EDX results.

Also, you could try doing your EDX first, then sputter-coating your samples
for imaging purposes. Of course, then you lose the capability of going
back to recheck your x-ray data.

If you must find a particular particle and image and do x-ray on it at the
same time, then let me know what you find out from others, because that's a
problem I've faced, too. The only thing that comes to mind immediately is
image it at low kV, then reconfigure your scope for higher kV backscatter
and EDX and hope you don't lose the particle in the process.

Best wishes,
Randy Tindall
Electron Microscope Laboratory
Box 3EML
New Mexico State University
Las Cruces, NM 88003




From: Lou Ann Miller :      ux1.cso.uiuc.edu-at-ux1.cso.uiuc.edu
Date: Fri, 19 Sep 1997 17:02:13 -0600
Subject: Re: BSE imaging of diatoms

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Moving the mercury story after the "air " time it has had I can see.

But , I do see a need to discuss gloves on this listserve, we work with too
many nasty chemicals. Especially with chemicals such as lowicryl.

Does anyone know of a WWW site for glove permiability information? Perhaps
even with microscopy specific chemicals?

I would like to make it a bookmark, or put an anchor to it on our web pages.

Thanks,

Lou Ann






} Can we move the Hg discussion to www.Hgtox.com, the gloves discussion to
} the handwear listserv, and get on to death of more important people (Di)?
}
} Seriously, Prof. Wetterhahn and Di were interesting individuals who have
} received their eulogies in other more appropriate forums. Whether one drop
} of diMeHg causes death is debatable, but not here (are any of you
} microscopists using it?). Gloves get holes and have unsealed wrists
} (usually). Enough OK?
} Rob Palmer
} CEB/UT


***************************
Lou Ann Miller
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Central States Microscopy Society
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/CSMS/csms.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/LAM.html






From: C. SHEN :      S922805-at-slvaxa.umsl.edu
Date: Fri, 19 Sep 1997 16:17:15 -0500 (CDT)
Subject: Re: Hg, gloves, death

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Dear Sir:

Whether it is proper or not to discuss the death of a scientist
in this list, I do not know.

BUT, I do not think a SCIENTIST's death is less valued than
those CELEBILITIES' or Di. Without scientists, our world
will less progress but without those "celebrities", our
world has no impact or even better !!

We are scientists and engineers, we should respect ourselves and
be proud of it.

Have a nice day!

Chang




From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 19 Sep 1997 19:39:49 -0400
Subject: Re: Mercury Poisoning

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I am not a chemist, but have received quite extensive
training on hazardous materials here at work. I have
learned that materials not normally absorbed through
the skin can be readily absorbed when they are carried
by a material that is absorbed. I believe that it may
amplify the toxic effects in some cases.

It is dangerous to assume that a material that presents
a minor hazard in one state, is not hazardous in another!

Darrell




From: Barbara Foster :      mme-at-map.com
Date: Fri, 19 Sep 1997 21:19:27 -0700
Subject: Re: Help with bse imaging of diatoms...

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David L Johnson wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.}
} I'm doing automated particle analysis (SEM/EDX) of suspended particulate
} material from drinking water reservoirs. Particles are filtered onto
} nuclepore membranes and carbon coated. Features are located using a
} thresholded bse image. Diatoms (some species) are so thin that that they
} don't image very well. Does anybody know of a 'staining' method to
} increase the atomic number contrast for amorphous silica?
}
} reply to jptmvl-at-mailbox.syr.edu thanx, dave johnsonDave,

I don't know of an EM approach but there is a very old technique called
"Rheinberg Illumination" which works a treat with diatoms. Basically,
the technique is called "optical staining" and involves selectively
filtering the background versus the scattered light. One of my favorites
produces a blue background and yellow diatoms .... a combination which
should be pretty effective for automated image analysis systems. We've
used them for years on many non-stained specimens, ranging from diatoms
to mold in ketsup.

The filters, originally available from KODAK, worked really well with 10x
objectives. When they discontinued producing these filters, they were
kind enough to give us the originals. We can make a set of about 20
different filters available to you for a modest price. Contact me
directly and I can provide you with the details for both how to use these
filters and pricing.

Plase let us know how you make out.

Best regards,

Barbara Foster
President
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email:mme-at-map.com
---------------------------------------------------------------------------------------------------------------------------------
********** Microscopy/Microscopy Education **********
America’s First National Consortium of Microscopy Experts
Specializing in Customized, On-site Training
in all areas of Microscopy, Sample Prep, and Image Analysis


What size are the particles you are trying to image?




From: MAPE-at-gnv.ifas.ufl.edu (Maureen A. Petersen)
Date: Fri, 19 Sep 1997 21:17:27 -0500
Subject: Hg, ...Sigh

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Microscopists and Fellow Listers:

For the first time I am going to add my two cents worth. In regards to the
posting and discussion about dimethylmercury, I would rather read about
safety problems and concerns than be subjected to the bickering between
list members. This is not the first occasion that some list members have
used this public forum to deliver jibes. That the occassion revolves
around someone's death makes it all the more objectionable to me. Although
Nestor has reminded the entire list of the rules of the list, and the
objectionable traffic has abated, I'm seeing it again. Please respect the
published purpose and rules of this list. I speak for myself, but perhaps I
am not alone in feeling this way.

Sincerely,
Maureen Petersen

************************************************************************
Maureen Petersen
Department of Plant Pathology
1453 Fifield Hall
University of Florida

voice: (352) 392-0634
fax: (352) 392-6532
email: MAPE-at-gnv.ifas.ufl.edu
************************************************************************






From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Fri, 19 Sep 1997 20:17:20 -0700
Subject: Re: Help with bse imaging of diatoms...

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Dear David,
A light gold or gold-palladium sputter coat will increase BSE response and will
interfere minimally with EDX identification. Diatoms are pure Si anyway, so
small Au and Pd peaks will not interfere. I always use Au-Pd when I image by
BSE, unless there are heavy elements already present.

David Johnson wrote:
} I'm doing automated particle analysis (SEM/EDX) of suspended particulate
} material from drinking water reservoirs. Particles are filtered onto
} nuclepore membranes and carbon coated. Features are located using a
} thresholded bse image. Diatoms (some species) are so thin that that they
} don't image very well. Does anybody know of a 'staining' method to
} increase the atomic number contrast for amorphous silica?
}
} reply to jptmvl-at-mailbox.syr.edu thanx, dave johnson
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: Gary Radice :      gradice-at-richmond.edu
Date: Sat, 20 Sep 1997 11:33:54 -0400
Subject: shelf life of Paraplast?

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I keep Paraplast Plus melted in a bath more or less continuously, so it is
always ready to use, even though it may be one or two months between uses.
Lately my students have been having some sectioning problems (with freshly
sharpened blades) and before I suggest that maybe they have done something
wrong during embedding, is it possible that the wax has "gone bad?"

Gary Radice, Associate Professor gradice-at-richmond.edu
Department of Biology 804-289-8107 (voice)
University of Richmond VA 23173 804-289-8233 (FAX)






From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Mon, 15 Sep 1997 08:45:12 -0400 (EDT)
Subject: Re: Immerse or float EM grids for staining?

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Dear Thomas,

I always immerse the grids for IEM-colloidial gold expt's. That is if
I am using only 1 primary antibody. If using 2 primary antibody, then I float
the grids, one side per antibody/gold.
As a side note, I also immerse the grids when staining with UA and Pb.

Best of Luck
Ed Calomeni
Medical College of Ohio
Dept Pathology
Toledo, OH 43614
emlab-at-opus.mco.ecu




From: dmatthew-at-providence.edu () (by way of Nestor J. Zaluzec)
Date: Mon, 15 Sep 1997 07:57:48 -0500
Subject: Help: fixation techniques for examaning the amebocytes of

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Colleagues , I can't help this guy can any of you? Reply back to
him not the listserver...

Nestor
Your Friendly Neighborhood SysOp



Below is the result of your feedback form. It was submitted by
(dmatthew-at-providence.edu) on Sunday, September 14, 1997 at 20:46:01
---------------------------------------------------------------------------

Email: dmatthew-at-providence.edu
Name: Doug Matthews

School: Providence College

State: RI

Zip: 02918

Question: Hello. I am an undergrad student at PC just beginning
a class using the EM. For a project I plan on examaning
the amebocytes of limulus polyphemus (horshoe crab).
I am intersted in any information on fixation
techniques of the cells for the TEM. I had planned
on centrifuging blood and then processing the
pellet. I've begun a literature search for specific
techniques, but getting my hands on the more obscure
journals can be difficult. Any help would be appreciated.
Thanx so much.

---------------------------------------------------------------------------






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 22 Sep 1997 09:59:57 +0100 (BST)
Subject: Re: Chromium

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Tom:

We got our target from Goodfellow Metals in Cambridge UK Tel:
+44-1223-568068 Fax: +44-1223-420639. They have high purity chromium
foils of various thicknesses and will laser cut to what ever size is
needed. We ordered a 1mm thick target for the Denton Magnetron Sputter
Coater we have on our Oxford Instruments CM1500 cryopreparation unit. It
works very well. Get Goodfellows to send you their very informative
catalogue.

Patrick Echlin
Director, Multi-Image Centre
University of Cambridge

On Wed, 10 Sep 1997, T. Graham wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
} I need to get ahold of a chromium sputtering target. Does anyone know
} which company I may purchase this from?
}
} Tom Graham
}
}
}





From: IMRE KOVACS M.D. :      KIMRE-at-comser.szote.u-szeged.hu
Date: Mon, 22 Sep 1997 11:08:06 +0100
Subject: Histoclear, Histomount

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

Does anyone know of the Histoclear and Histomount clearing and
mounting media? I'd like to know the name of the manufacturer and
where they are located?
Thanks,


Imre Kovacs M.D.
kimre-at-comser.szote.u-szeged.hu

Alzheimer's Disease
Research Center
Albert Szent-Gyorgyi
Medical University
H-6720 Szeged,
Somogyi u. 4.
Hungary





From: bozzolo-at-crpcu.lu (Nathalie Bozzolo)
Date: Mon, 22 Sep 97 11:11 MET DST
Subject: TEM - holey carbon films

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Dear colleagues,
I have formvar powder and choloform at my disposal to prepare holey carbon
films. Could you give me the method to use or the references of some
literature where I can find it?
Thank you for your help! Nathalie Bozzolo
_________________________________________________

Nathalie Bozzolo
Laboratoire d'Analyse des Materiaux
Centre de Recherche Public - Centre Universitaire
162a, avenue de la Faiencerie
L-1511 Luxembourg
tel : (352)46 66 44 402
fax : (352)46 66 44 400
_________________________________________________





From: Robert H. Olley :      R.H.Olley-at-reading.ac.uk
Date: Mon, 22 Sep 1997 12:15:53 +0100 (BST)
Subject: Heavy metals (Pb, Bi)

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Many thanks to Phil Dahlstrom for sorting out the "mercury" confusion. It
reminds me of the time when I was an inexperienced young scientist at the
Paint Research Association (and dinosaurs walked the earth!). I had
thought of using tetraethyl lead as an (electron? X-ray?) dense material,
and was forcefully warned off by one of the senior staff there. Simply
because it was found in petrol did not mean that it was OK to use: in
fact, at the "lead" factory there would be paraffin (kerosene) showers,
and on the slightest contact the victim would be shoved under, clothes and
all - speed is of the essence.

More recently, I was looking for ways of putting heavy metal into
specimens either for TEM density or for backscattering SEM, and I
discovered recent work in the literature, suggesting triphenyl bismuth as
a relatively low toxicity material - bismuth compounds are apparently
remarkably innocuous compared to its neighbours in the periodic table. I
didn't get so far with that, as we found another way of imaging our
specimens - water trees in electric cables - but if anybody is interested,
here are a couple of references they could follow up:


(1) TI: THERMOMECHANICAL INVESTIGATION OF POLY(METHYLMETHACRYLATE)
CONTAINING AN ORGANOBISMUTH RADIOPACIFYING ADDITIVE
AU: RAWLS_HR, GRANIER_RJ, SMID_J, CABASSO_I
JN: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1996, Vol.31, No.3,
pp.339-343

(2) TI: Radiopaque copolymers of styryldiphenylbismuth
vinylbenzylphosphonate and methyl methacrylate
AU: Tamber_H, Smid_J, Cabasso_I
JN: CHEMISTRY OF MATERIALS, 1997, Vol.9, No.6, pp.1335-1341


+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+





From: XraySci-at-aol.com
Date: Mon, 22 Sep 1997 08:14:41 -0400 (EDT)
Subject: Help High voltage adjust on Kevex 8000

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Dear Microscopists,

How do I adjust the High Voltage on my Kevex 8000 analyzer. Is this
acomplished through Software or Hardware.


Thank You
Keith Brenna




From: Harrison, Gail :      Gail.Harrison-at-reichhold.com
Date: Mon, 22 Sep 1997 08:32:21 -0400
Subject: RE: Hg, gloves, death

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I second the motion.

} -----Original Message-----
} From: Vladimir Dusevich [SMTP:dusevich-at-astro.ocis.temple.edu]
} Sent: Friday, September 19, 1997 2:56 PM
} To: Robert J. Palmer Jr.
} Cc: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Re: Hg, gloves, death
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} ----------------------------------------------------------------------
} -.
}
} 100% agree!!!
}
} On Fri, 19 Sep 1997, Robert J. Palmer Jr. wrote:
}
} }
} ----------------------------------------------------------------------
} --
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} }
} ----------------------------------------------------------------------
} -.
} }
} } Can we move the Hg discussion to www.Hgtox.com, the gloves
} discussion to
} } the handwear listserv, and get on to death of more important people
} (Di)?
} }
} } Seriously, Prof. Wetterhahn and Di were interesting individuals who
} have
} } received their eulogies in other more appropriate forums. Whether
} one drop
} } of diMeHg causes death is debatable, but not here (are any of you
} } microscopists using it?). Gloves get holes and have unsealed wrists
} } (usually). Enough OK?
} } Rob Palmer
} } CEB/UT
} }
} }
} }




From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Mon, 22 Sep 1997 10:19:11 -0400
Subject: INVITATION TO SUBMIT PROPOSALS

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INVITATION TO SUBMIT PROPOSALS FOR
1) PROGRAM PARTICIPATION and
2) 1998 FACULTY FELLOWSHIPS

The Shared Research Equipment (SHaRE) User Facility and Program at the Oak
Ridge National Laboratory (ORNL) provides access to a variety of advanced
instrumentation for collaborative materials science research. Through the
SHaRE User Program, materials scientists from universities, industries, or
other government laboratories may access the SHaRE Facility as well as other
instrumentation within ORNL's Metals and Ceramics (M&C) Division. Facility
instrumentation includes a variety of electron microscopes, atom probe
field-ion microscopes, and mechanical properties microprobes.

SHaRE Program Participation
Proposals are being solicited at this time for facility use during fiscal
year 1998 (October 1, 1997 - September 30, 1998). The program is intended
to support collaborations between M&C staff members and researchers external
to ORNL. Therefore, proposals must identify at least one staff member and
one non-ORNL participant who will act as a co-principal investigator and
share responsibility for the project. Proposals will be reviewed by a
committee and evaluated with regard to scientific excellence, relevance to
the interests of the U.S. Department of Energy, Division of Materials
Sciences, and the likelihood of project success. Additionally, principal
investigators and graduate students from U.S. accredited universities are
eligible to receive funds to defray certain program-related travel and
subsistence expenses.

The SHaRE Facility instrumentation and the related User Program are
described in detail at http://www.ornl.gov/share. In the past, only letter
proposals were submitted for program participation. However, application
for program participation and travel support is now made by using a
downloaded form located at http://www.ornl.gov/share/pdf/proposal98.pdf.
The form will be mailed to potential applicants upon request.

1998 Faculty Fellowships
Faculty fellowships provide outstanding university faculty extended access
to the SHaRE User Facility at ORNL. It is anticipated that at least one
junior and one senior university faculty member will be appointed as
fellows. Information regarding the fellowships, including eligibility
requirements, length of appointment, stipends, and application guidelines
may be found at http://www.ornl.gov/share. The guidelines will be mailed to
potential applicants upon request.

SHaRE will accept and review proposals for projects and fellowships at any
time during the fiscal year, but allocates the majority of funds during the
month of October. Proposals for review during the October meeting should be
received at the address below by October 10, 1997. Proposals will be
reviewed, and travel awards announced, in mid-October. Fellowship
applications received after the October date will be reviewed during
February 1998.

Proposals submitted (5 copies) should be sent to:

SHaRE Proposals
Education and Training Division, MS-36
Oak Ridge Institute for Science and Education
P.O. Box 117
Oak Ridge, Tennessee 37831-0117

SHaRE is jointly administered for the U.S. Department of Energy by ORNL and
the Oak Ridge Institute for Science and Education (ORISE). For additional
information or clarification on proposal submissions, please contact me
directly.

Regards,

Neal





Dr. Neal D. Evans voice: (423) 576-4427
Shared Research Equipment Program facsimile: (423) 574-0641
Oak Ridge National Laboratory email: evansnd-at-ornl.gov
Building 5500, MS 6376
Oak Ridge, TN 37831-6376





From: José Luis Encinas :      encina1-at-ibm.net
Date: Mon, 22 Sep 1997 18:47:23 -0700
Subject: SEM_EDX - New member

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Hi,
I am a new member of this listserver. I have a page dealing with SEM-EDX
applied to quality control of food and industrial product. The URL is:

http://www.geocities.com/CapeCanaveral/Lab/1987

I will consider any comment.

I would like to exchange experiences in the field of quality control.

- Best regard -

Jose Luis Encinas
Head of Electron Microscopy
Centro de Investigacion y Control de la Calidad
Ministry of Health
Av. Cantabria s/n 28042 Madrid Spain




From: Julia Polak :      jpolak-at-esu6.esu6.k12.ne.us
Date: Mon, 22 Sep 1997 12:08:07 +0000
Subject: Protist pix

Contents Retrieved from Microscopy Listserver Archives
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Greetings!
I am looking for a site where I can find microscopic pictures of
various kinds of protists for my fifth grade science students.
I have drawings, but we do not have access to slides of the real
thing. Perhaps someone out there can direct me to a useful website
that will interst my students. Thanks!
Reply to: jpolak-at-esu6.esu6.k12.ne.us
Julie Polak
Exeter Public Schools
Exeter, Nebraska




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Mon, 22 Sep 1997 12:14:32 -0600
Subject: Re: Collecting and fixing yeast for SEM

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} I have to prepare some yeast for SEM. The person who requested this
} work wants some pretty pictures of his yeast, Schizosaccharomyces pombe,
} for use in seminars. I have read up on some techniques to use but have
} two main queries:
} 1. To collect the yeast onto filter paper ( a method used in
} several publications), do I just drop a suspension of the yeast onto the
} filter paper? What sort of filter paper do I use? Is there a "better"
} way of collecting these cells such as settling them onto poly-l-lysine
} coverslips?

We use regular microscope slides, cleaned well (detergent, acid or
alcohol): coat with l mg/ml aqueous solution of poly-L-lysine for several
minutes, rinse in distilled wate.

Take a (preferably) aqueous suspension of the yeast and place onto the
microscope slide and allow the cells to settle for about one hour at RT.
Carefully tip the slide and allow the unattached cells to flow off. Now,
gently dropper some fixative (2% glut/4% formald in buffer of choice) onto
the slide to completely cover the smear. Allow to set undisturbed overnight
at RT in a humid chamber (petri dish with filter paper or Tupperware
container with moist paper towels). Transfer the slide into a rinse
solution (Coplin jar of distilled water or petri of distilled water). With
some yeasts, the aldehyde fix is adequate to preserve the integrity of the
cells (others may collapse without osmium post-fixation). The slides are
then slowly dehydrated in ethanol (20-40-60-80-100-100%) for 10 min each.
Critical point dry using liquid carbon dioxide as transitional solvent. You
will need to break the slide into 1 inch squares to fit into the CPD
device. Do this by scoring the slide with a diamond marker pen and pressing
down gently onto an applicator stick. Mark an "X" the back side with the
diamond marker to help identify the good side later. Freeze drying from
the ethanol should also work well.


IF osmium is needed place slide/specimen into 2% aqueous osmium tetroxide
overnight - take care to avoid evaporation by either immersion of the slide
into a shallow container of osmium or by vapor fixation of the
slide/specimen. Vapor fixation (CAUTION WITH OS FUMES - DO IN PROPERLY
OPERATING FUME HOOD) may be accomplished by placing the wet slide/specimen
into a plastic petri dish and then placing a small volume (4-5 ml) of 2%
osmium solution nearby in the dish. Rinse in distilled water and dehydrate
and CPD as described above.

Caveats: do not overload the slides with culture since you do not want a
heaped up mess but isolated cells. A suspension that is quite turbid should
work well - not a paste or milky/opaque solution.

Contact me if you have any other questions. I have done a lot of imaging of
yeasties by TEM and SEM.

Cheers,



####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Neckers Building, Room 146 - B Wing
Southern Illinois University
Carbondale, IL 62901
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: Jolanta Mesjasz-Przybylowicz :      mesjasz-at-srvnac1.nac.ac.za
Date: Mon, 22 Sep 1997 19:13:38 +0000
Subject: cryomicrotomy of leaf

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Dear Microscopists,


I would like to ask you for your advise on cryo-sectioning of plant
material in possibly low temperature. We tried to section the frozen
piece of leaf using Cryocut E Reichert, playing with different embedding
media but the results were not satisfactory. We found difficulties in
cutting thick sections (5-30 microns) even of very young leaf.
Morphological structure was not well preserved.

I really would appreciate your advise, suggestions, tips and
technical tricks in this matter.
What equipment do you use and what can you recommend?

Thank you in advance for all your time and assistance

With best regards

Jolanta Mesjasz-Przybylowicz
************************************************************************
Dr Jolanta Mesjasz-Przybylowicz
National Accelerator Centre
P.O. Box 72
Faure 7131
South Africa
tel: 27-21-8433820
fax: 27-21-8433543
Internet: MESJASZ-at-nac.ac.za
************************************************************************




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Mon, 22 Sep 1997 12:31:50 -0600
Subject: Re: Histoclear, Histomount

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I have used both with excellent results, for 10 micron and 100-200 micron
sections. Histomount *really* likes to shrink, so it needs to be watched
while drying. Also, it makes zillions of air bubbles if you try to dry it
with heating like is done with Permount. Dry finished slides at room temp.
Excess Histomount cleans up with Histoclear and a razor blade.

Both are made/sold by National Diagnostics in New Jersey, US, but I don't
have the contact information. (This is a year old--I assume the information
hasn't changed.)

Fisher sells a similar (identical?) product to Histoclear, but I don't
remember if they have a Histomount analog.

Phil

} Does anyone know of the Histoclear and Histomount clearing and
} mounting media? I'd like to know the name of the manufacturer and
} where they are located?
} Thanks,
}
}
} Imre Kovacs M.D.
} kimre-at-comser.szote.u-szeged.hu
}
} Alzheimer's Disease
} Research Center
} Albert Szent-Gyorgyi
} Medical University
} H-6720 Szeged,
} Somogyi u. 4.
} Hungary

}}}}}}}}}}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{{{{{{{{{{

Philip Oshel
Station A
PO Box 5037
Champaign, IL 61825-5037
(217) 355-1143
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again *****







From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Mon, 22 Sep 1997 13:45:40 -0500
Subject: RE: TEM - holey carbon films

Contents Retrieved from Microscopy Listserver Archives
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This is the method that we use at the Health Sciences Centre here in
Winnipeg. I have reasonable holey carbon grids made with this method.

Solution a: 125mg formvar in 50ml chloroform
Solution b: 50% water, 50% glycerol

1. Add 4 drops of solution b to solution a, and shake vigorously for 30
seconds.
2. Pour on clean glass slides and dry. (ie: dip the slides into a
staining dish.)
3. Breath on slide several times.
4. Float layer off the slides.
5. On the floating layers, gently place grids, dull side down.
6. Pick up layer with parafilm, letting the film stick to the
parafilm.
7. Let dry for 30 minutes.
8. Put in petri dish with filter paper saturated with 50% methanol for
20 minutes.
9. Take each grid off mesh and dip in 50% methanol and place on filter
paper to dry.
10. Coat with carbon in a vacuum evaporator.
11. Immerse each grid in chloroform briefly.

} ----------
} From: bozzolo-at-crpcu.lu[SMTP:bozzolo-at-crpcu.lu]
} Sent: 22 September, 1997 06:11
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: TEM - holey carbon films
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 22 Sep 1997 12:56:26 -0700 (PDT)
Subject: Re: Histoclear, Histomount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi The company is:National Diagnostics 404-699-2121

bob

On Mon, 22 Sep 1997, IMRE KOVACS M.D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Dear Microscopists,
}
} Does anyone know of the Histoclear and Histomount clearing and
} mounting media? I'd like to know the name of the manufacturer and
} where they are located?
} Thanks,
}
}
} Imre Kovacs M.D.
} kimre-at-comser.szote.u-szeged.hu
}
} Alzheimer's Disease
} Research Center
} Albert Szent-Gyorgyi
} Medical University
} H-6720 Szeged,
} Somogyi u. 4.
} Hungary
}
}





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Mon, 22 Sep 1997 20:56:11 +0100
Subject: Re: Mercury Poisoning Story

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} Rick,
} While the AP is not peer reviewed Science is. Suggest that you check out
} the August issue. Karen
} was a collaberator with several people in our lab. Also suggest that
} you look up
} "dimethylmercury". One drop (50ul) is equal to about 300X the
} occupational exposure limit.
} -- Begin original message --
} } Without in any way downplaying the tragedy for the people involved, the
} } dose-response relationship postulated in the story is not very plausible.
} } Mercury toxicity has been studied extensively for at least half a century.
} } It is a cumulative poison which does cause the type of neurological effects
} } reported, but a "single tiny drop", through a glove and quickly washed off,
} } being lethal seems suspect. Associated Press is not peer reviewed (although
} } maybe it depends on your definition of "peer").
} }
} } Rick Mott
} } rick-at-pgt.com
} }
} } (These are personal views having nothing to do with my employer)
} }
}
} -- End original message --
}
}
} regards,
} Bob
} Robert Schoonhoven

Of course, one of the problems is that the original newspaper article that
started this thread refered to 'mercury'. Which as many have pointed out,
as elemental metallic mercury while dangerous is essentially a long term,
cumulative poison and there is no possibility that single drop on even
unprotected skin will cause the slightest harm.

On the other hand, dimethylmercury is an entirely different kettle of fish
(apologies for colloquialism). This is a classic example on the confusion
that arises when the scientifically ignorant start talking about things
they don't understand.

It reminds me of the debate in the British parliament a number of years ago
on the merits of fluoridation of water - the principle case was made by a
scientifically ignorant MP who based his argument against fluoridation on a
dictionary definition of the chemical and biological properties of
fluorine. One wonders if he had ever looked up the same information for
chlorine and took a similar stand on his use of salt on his food.

While there are many arguments in science, particularly in areas relating
to ethical issues, which may be open to the general public, there are
equally many that aren't. It is simply a case that if you haven't studied
and learnt the basic facts, you are ignorant and aren't qualified to
comment. And notions of democracy, and free speech don't change that.

Regards,
Larry Stoter






From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 22 Sep 1997 17:16:00 -0400
Subject: Position open

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The Division of Nephrology in the University of Florida College of
Medicine is seeking a full-time electron microscopy technician. This
individual will be one of two full-time electron microscopy technicians
staffing the Division's Electron Microscopy Facility, which serves as a
research facility for the members of the Division of Nephrology and
their collaborators. The research conducted primarily strives to
determine correlations between renal ultrastructure and function using
transmission and scanning electron microscopy, morphometric analysis, and
immunogold and immunoperoxidase cytochemistry. Equipment housed within
the facility include 2 Zeiss EM-10A transmission electron microscopes, a
Topcon DS130C scanning electron microscope, 2 LKB Nova ultramicrotomes,
a Leica Automatic Freeze Substitution unit, and all related support
equipment. The candidate's duties will include tissue processing,
ultramicrotomy, immunocytochemistry, routine maintenance of the electron
microscopes, viewing samples, photographic processing, and related
support functions. The candidate may be hired as an Electron Microscopy
Technician of Senior Electron Microscopy Technician depending on
experience.

Reply to Dr. Jill Verlander verlandr-at-medicine.ufl.edu
*******************************************************
G.W. Erdos, Ph.D. Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
PO Box 118525 Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/
Home of the #1 Gators
*****
"Many shall run to and fro, and knowledge shall be increased"
Daniel 12:4





From: UTC Engineering Division 2 :      utc-en2-at-erinet.com
Date: Mon, 22 Sep 1997 17:35:01 -0400
Subject: TEM & Microelectromechanical

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TEM & Microelectromechanical

Our Ohio company is seeking engineering / scientist support for
microelectromechanical systems research & development. Work will involve
new materials and surface treatments to control the friction and wear of
MEMS devices; Friction & wear measurement of MEMS systems / materials.

It is important that candidates have capabilities in cross-section TEM,
analytical TEM of tribological materials; especially wear tracks; creation
of unique microstructures; and understand friction & wear on a fundamental
level.


Contact Ronald Decker - deckerrc-at-utcdayton.com



Ronald C Decker
Program Manager
Universal Technology Corporation
1321 Research Park Drive, Suite 100
Dayton OH 45432-2817
Voice (937) 426-8530, Fax (937) 426-7753






From: Peter Jordan :      emsi-at-pe.net
Date: Mon, 22 Sep 1997 20:54:12 -0700
Subject: Used Jeol 100ZX for sale

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Dear All:
I inherited a used Jeol 100ZX. It is in excellent working condition and
about 20 years old, located in the Los Angeles area. If you are in need
of a TEM or want it for spare parts please make me an offer.
Peter Jordan, EMSI 909 694-1939




From: egautier-at-labs.polycnrs-gre.fr
Date: Tue, 23 Sep 1997 08:17:26 +0200 (MET DST)
Subject: job or financial support for postdoctoral

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Hi,
wanted job or financial support for Postdoctoral
keywords: TEM, HREM, EDX, EELS, Diffraction, Crystallography, Physics, Material
thanks


Eric GAUTIER
CNRS Cristallographie
BP 166
38042 Grenoble cedex 9
tel. 04 76 88 74 19
fax 04 76 88 10 38






From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Tue, 23 Sep 1997 08:20:48 +0200 (GMT+0200)
Subject: Re: Histoclear, Histomount

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This information is correct as of March 95 when we last ordered. A
European source of Hystomount (and I think also Hystoclear) is:

Hughes and Hughes Ltd.
Unit 1F, Lowmoor Industrial Estate,
Tonedale, Wellinton,
Somerset TA21 0AZ
England
Phone +44 823 660222
FAX +44 823 660186

As regards to Hystoclear, there are other companies which sell a
limonene-based xylene substitute. As an example, Fisher calls its product
Hemo-De. They are different than xylene in more than just the toxicity.
Thus I would ask for a small sample from your supplier to try before you
buy. In my experience these products are normally sold in large quantities.
(Fisher lists it smallest size as 1 gallon).

Good luck,
Azriel Gorski

On Mon, 22 Sep 1997, IMRE KOVACS M.D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Dear Microscopists,
}
} Does anyone know of the Histoclear and Histomount clearing and
} mounting media? I'd like to know the name of the manufacturer and
} where they are located?
} Thanks,
}
}
} Imre Kovacs M.D.
} kimre-at-comser.szote.u-szeged.hu
}
} Alzheimer's Disease
} Research Center
} Albert Szent-Gyorgyi
} Medical University
} H-6720 Szeged,
} Somogyi u. 4.
} Hungary
}





From: Patrick Huddie :      phuddie-at-microcosm.com
Date: Tue, 23 Sep 1997 06:34:45 -0400
Subject: Axioplan wanted

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Dear All:

We need a Zeiss Axioplan microscope for transmitted light and
fluorescence work. This would be the original Axioplan, not the new
Axioplan 2 model. If you own this microscope, and are interested in
selling or trading your instrument, please send me details of the
configuration you have. Part numbers would be helpful.

Patrick

--------------------------------------------------------------
Dr. Patrick L. Huddie
(301) 725-2775
Fax (301) 725-2941
Microcosm, Inc., 9140 Guilford Road, Suite O, Columbia, MD 21046
e-mail phuddie-at-microcosm.com URL http://www.microcosm.com







From: Carlos E. Barbosa :      grial-at-satlink.com
Date: Tue, 23 Sep 1997 08:21:49 -0300
Subject: LM: video board

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I want to attach a video system to my light microscope. I just bought
the camera (Sony ccd 370) and the corresponding adaptor and lens,
although now I'm having trouble with the board. I bought a miro dc30
board but I only get a small image (I want the image on the entire
screen), and, besides, I'm not having good printings. I'm working with
and HP 133 Mhz, 48 MB ram, and my printer is an HP 870. I guess that
this are good working conditions, thus, I believe that the board is not
correct.
Can anyone help me?
Thank you in advance!

Carlos Barbosa





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Tue, 23 Sep 1997 08:40:49 -0500
Subject: Re: LM: video board

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Greetings, Carlos,

I would check out the adapter and lens system before the board.

We have a Pixera camera that we have tried to replace our old RS-170 camera
with. The lens/adapter for the RS-170 camera screws right in to the forn of
our Pixera, however, the field of view is only about one third the size it
was with our RS-170 camera. I came to find out that that should not be a
surprise. The chip on the Pixera is only 1/3" across while it is 1" across
on the RS-170. We have yet to get the right adapter, but have looked at
items from Optem, Diagnostic Instruments, and Edmund Scientific. We should
be able to get much closer.

There are others out there that have gone through the same exercise. Maybe
they will speak up too.

At 08:21 AM 9/23/97 -0300, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.marl.iastate.edu/marl/ (re: SEM)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)

electron microscopy, x-ray analysis, image analysis
computer applications
coal characterization and processing





From: zhang-at-cvlab.harvard.edu (Dorothy Zhang)
Date: Tue, 23 Sep 1997 10:01:18 -0500
Subject: Confocal and ultramicritome for sale

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Dear microscopists:
We have very good deal of barely used, looks like new NORAN confocal and
RMC ultramicrotome and their accesories for sale. Please contact Dorothy at
617-432-2970. Thanks.

Dororhty zhang






From: Kalman Rubinson :      rubinsnk-at-is2.nyu.edu
Date: Tue, 23 Sep 1997 11:19:29 -0400 (EDT)
Subject: Re: LM: video board

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On Tue, 23 Sep 1997, Carlos E. Barbosa wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} I want to attach a video system to my light microscope. I just bought
} the camera (Sony ccd 370) and the corresponding adaptor and lens,
} although now I'm having trouble with the board. I bought a miro dc30
} board but I only get a small image (I want the image on the entire
} screen),

Not necessarily. I am not familiar with your camera or board but the
problem could be that the relay lens for the camera has too large a field
of view. Have you tried connecting the camera directly to a monitor? Is
the image acceptable on that?

} and, besides, I'm not having good printings.

What does that mean?

Kal





From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Tue, 23 Sep 1997 08:36:15 -0700 (PDT)
Subject: My Denton Sucks = )

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BBers,

Thanks to all who responded to my question about my Denton not sucking.
The problem turned out to be a broken rough pump bellows.
Ida (the parts lady) at Denton was great! Even though there was a
long backorder for the part, she searched all over Denton and found a
bellows for me. She did this out of the kindness of her heart. Thanks to
her we were able to repair my machine lickety-split.
I just thought I'd let everybody know how much I appreciate them,
the BBer's for advice and the Denton folks for being so helpful.
My Denton now sucks like you wouldn't believe. It hasn't worked
this well in a long time. It can now pump down to 2 X 10-5 without any LN2
added to the trap.
I guess the moral of the story is...It always pays to ask those who
know.


Happily carbon coating in Berkeley,


Paula = )

p.s. I have no financial interest in Denton, etc, etc, etc.

Paula Sicurello
UC Berkeley
Electron Microscope Lab
psic-at-uclink4.berkeley.edu






From: Woody.N.White-at-mcdermott.com
Date: 9/23/97 6:06 AM
Subject: LM: video board

Contents Retrieved from Microscopy Listserver Archives
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I am not familiar with the Sony system, but if it is NTSC video, then
you will be limited to about 500 lines of vertical resolution.
Horizontal resolution will depend on the Sony and the capture card.
It is probably about 400 lines... This will equate to 500x~400 pixel
image. If your computer screen is set to - say 1024x768 pixel display,
the unmodified image would fill about 1/2 the CRT. There may not be a
convenient way to increase the Sony resolution, but you could fill
the screen by reducing the CRT resolution to 640x480. Better yet,
(although "hollow" magnification) would be to increase the image pixel
array size (software manipulation) after capture to fill the screen.
Increasing the pixel array for the same size hard copy may help
produce better halftone printer output also.

For work like this, I would prefer higher resolution, digital
still cameras. The obvious drawback is lack of "real-time" output.

Woody White, Electron Microscopist SEM/EDS/WDS

Work:
Mcdermott Technology, Inc.
woody.n.white-at-mcdermott.com
http://www.mtiresearch.com/

Home:
woody.white-at-worldnet.att.net
http://www.geocities.com/capecanaveral/3722

______________________________ Reply Separator
_________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I want to attach a video system to my light microscope. I just bought
the camera (Sony ccd 370) and the corresponding adaptor and lens,
although now I'm having trouble with the board. I bought a miro dc30
board but I only get a small image (I want the image on the entire
screen), and, besides, I'm not having good printings. I'm working with
and HP 133 Mhz, 48 MB ram, and my printer is an HP 870. I guess that
this are good working conditions, thus, I believe that the board is not
correct.
Can anyone help me?
Thank you in advance!

Carlos Barbosa




From: tflore-at-lsumc.edu (Flores, Teresa)
Date: Tue, 23 Sep 1997 14:41:24 -0500
Subject: immunofluorescent techniques

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X-Sender: tflore-at-pop3.lsumc.edu
Message-Id: {v01510102b04d849ce41e-at-[155.58.72.72]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: histonet-at-pathology.swmed.edu

Our lab is in the transition of doing immunogold tagging in HRLM-TEM.But we
continue to do immunofluorescent techniques received in Michels transport
media. Is there anyone out there still doing immunofluorescent techniques
(direct or indirect immunofluorescent techniques) on renal, skin, muscle or
nerve biopsies? If yes, is the biopsy snap-frozen in liquid nitrogen as
soon after removal as possible (precooling a metal chuck for about a
minute. Placing a small volume of saline or water on top of the colled
chuck, immediately placing the renal or skin bx into the water or saline
drop. As the water and the bx begin to freeze, the chuck is turned down to
eliminate excess water or saline. The chuck is placed in liquid nitrogen
for approx one minute to snap-freeze the bx or tissue. When nitrogen stops
bubling bx is adequately frozen and bx is ready to be stored at -70oC or to
be sectioned).
If not possible to do technique is the tissue being storred in Michels'
transport media also sold commercially as Zeus.
Are the renal biopsies being tagged with IgG, IgM, IgA, C3, C1q, Kappa & Lambda?
Skin, muscle and nerve biopsies tagged with IgG, IgM, IgA, C3 and Fibrinogen?
Please respond. Thanx Teresa






From: tflore-at-lsumc.edu (Flores, Teresa)
Date: Tue, 23 Sep 1997 14:41:24 -0500
Subject: immunofluorescent techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our lab is in the transition of doing immunogold tagging in HRLM-TEM.But we
continue to do immunofluorescent techniques received in Michels transport
media. Is there anyone out there still doing immunofluorescent techniques
(direct or indirect immunofluorescent techniques) on renal, skin, muscle or
nerve biopsies? If yes, is the biopsy snap-frozen in liquid nitrogen as
soon after removal as possible (precooling a metal chuck for about a
minute. Placing a small volume of saline or water on top of the colled
chuck, immediately placing the renal or skin bx into the water or saline
drop. As the water and the bx begin to freeze, the chuck is turned down to
eliminate excess water or saline. The chuck is placed in liquid nitrogen
for approx one minute to snap-freeze the bx or tissue. When nitrogen stops
bubling bx is adequately frozen and bx is ready to be stored at -70oC or to
be sectioned).
If not possible to do technique is the tissue being storred in Michels'
transport media also sold commercially as Zeus.
Are the renal biopsies being tagged with IgG, IgM, IgA, C3, C1q, Kappa & Lambda?
Skin, muscle and nerve biopsies tagged with IgG, IgM, IgA, C3 and Fibrinogen?
Please respond. Thanx Teresa






From: Kalman Rubinson :      rubinsnk-at-is2.nyu.edu
Date: Tue, 23 Sep 1997 15:59:56 -0400 (EDT)
Subject: Re: LM: video board

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 23 Sep 1997, Warren Straszheim wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
} I would check out the adapter and lens system before the board.

Agreed.

} We have a Pixera camera that we have tried to replace our old RS-170 camera
} with. The lens/adapter for the RS-170 camera screws right in to the forn of
} our Pixera, however, the field of view is only about one third the size it
} was with our RS-170 camera. I came to find out that that should not be a
} surprise. The chip on the Pixera is only 1/3" across while it is 1" across
} on the RS-170. We have yet to get the right adapter, but have looked at
} items from Optem, Diagnostic Instruments, and Edmund Scientific. We should
} be able to get much closer.

Have you tried other projective eyepieces?

Kal





From: zhang-at-cvlab.harvard.edu (Dorothy Zhang)
Date: Tue, 23 Sep 1997 15:28:57 -0500
Subject: Re: LM: video board

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Hi Marek:
The price of RMC ultramicrotome is $10K. We don't have cryo-version. Dorothy

Dororhty zhang






From: Ashok Krishnan :      krishnan-at-engr.latech.edu
Date: Tue, 23 Sep 1997 18:22:43
Subject: Which Technique to use? (long)

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I am not sure which particular kind of microscopy will
suit my needs, and would very much appreciate it if somebody
could help me in this regard.

My application is as follows:
I need to look at molecular-level changes occurring at a
gas-liquid interface with respect to some parameters. The liquid
will have, among other components, proteins in it. I want to look at
the larger molecules which are affected by an interface. For this, I
plan to take a liquid volume and disperse bubbles in them. Then I
plan to freeze a part of the liquid rapidly and carry out imaging at
various sections. I don't know what kind of technique or
microscopy is the best for this. I would appreciate comments and
suggestions on this.
Also, if somebody knows of a paper or book that has this
kind work in it, then that would give me some leads. I have
searched some of the indexes with keywords like surface, bubble,
modification, microscopy, protein etc., but could not find what I
wanted. Am I missing something here?
We have some generic metrology equipment like Surface
profilers (contact and Optical) , SEM, AFM, Light Microscopes
(not near-field). I would also be interested in knowing if any of this
can be adapted for my needs. I have NOT done any of the
following: biological tissues examining/sectioning, staining,
fixing, cryomicroscopy, AFM etc. My experience is with
microfabricated structures and examination under the SEM, light
microscope and surface profilers. However, I am very willing to
learn a new field, and can work towards obtaining a new piece of
equipment, or try to have some arrangement with interested
commercial/non-commercial parties.

Thanks very much.

Ashok
Institute for Micromanufacturing
Louisiana Tech University
E-mail: krishnan-at-engr.latech.edu
ph: (318) 251-8110, fax: (318) 257 5104

"Smaller, lighter, more functional and less expensive consumer
products, industrial machines, instruments...possibilities limited
only by man's imagination"





From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 23 Sep 1997 17:26:30 -0700
Subject: Re: Protist pix

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Ms. Polak:
Please look at the website list that is section V of the Project
MICRO bibliography (address below). And if you're really into protozoa, I
recommend the recent book by Anderson & Druger listed in section IIB.


Caroline Schooley
Educational Outreach Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/






From: Doty, Stephen Ph.D. :      DotyS-at-hss.edu
Date: Tue, 23 Sep 1997 19:27:12 -0500
Subject: Job opening

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The Analytical Microscopy Core facility at Hospital for Special
Surgery, New York, NY, is searching for a full time electron microscopy
technician. The Core provides TEM and SEM service to about 50 clinical and
basic scientists, whose major interests are in orthopedics and connective
tissue diseases. About 2 days per week would be used to provide technical
assistance to a senior researcher, which would involve TEM, SEM, immuno and
histochemistry, and development of image analysis techniques. This
individual should be familar with basic TEM and SEM techniques but will be
trained to work with connective tissues and calcified cartilage and bone.
The job can be classified as EM Technician or Senior Technician, depending
on qualifications. Interested persons please respond to Steve Doty at
address below:

Stephen B. Doty, PhD.
Phone: (212) 606-1417
Director, Analytical Microscopy Core Fax: (212)
717-1192
Hospital for Special Surgery
email: dotys-at-hss.edu
535 E. 70th Street, NY, NY 10021






From: David Brown :      keswick-at-rmplc.co.uk
Date: Tue, 23 Sep 1997 19:28:27 -0500
Subject: Olympus G type eyepieces

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Can any point me in the direction of a supplier (preferably used) of
Olympus G type eyepieces for an Olympus VT-II stereo microscope?

Many thanks, David






From: Peter Jordan :      emsi-at-pe.net
Date: Tue, 23 Sep 1997 18:23:52 -0700
Subject: Used Jeol 100C for sale

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Dear All:
I made two mistakes on my posting yesterday, first the Jeol is a 100C
and not a 100ZX and my phone number is 909 694-1839 and not 1939. The
thing I got right was that it is 20 years old and it is still for sale.
Sorry, I'll never write an e-mail letter at midnight.
Peter Jordan, EMSI




From: Peter Jordan :      emsi-at-pe.net
Date: Tue, 23 Sep 1997 21:55:45 -0700
Subject: Used Zeiss 9 TEM

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Dear All:
A few months ago there was a posting about a Zeiss 9 TEM given away for
free. If this is still available or if you know who did the posting
please let me know. If I remember right it was in the San Diego area.
Thank you.
Peter Jordan




From: Malgorzata.Warmuzek :      mwarm-at-czapla.IOd.krakow.pl
Date: Wed, 24 Sep 1997 10:32:50 +0200 (MET DST)
Subject: users of link izis 300

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I have a problem with an import the file with an extension *.sp ( spectrum )
and put it to the document in word 6 or amipro

Malgorzata Warmuzek
Foundry Research Institute
Research Materials Department
Structural and Physical Research Laboratory

Zakopianska 73 Call +48 12 2605022 ext. 317
30-418 KRAKOW - POLAND Fax +48 12 2665478, +48 12 2660870







From: Julia Polak :      jpolak-at-esu6.esu6.k12.ne.us
Date: Wed, 24 Sep 1997 08:14:34 +0000
Subject: protist pix

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Greetings again!
I want to thank all of you terrific people out there who have so
graciously sent me information about protist pictures!!!!
I took my 5th graders to several of the sites and they said the
protists were "AWESOME!!!" (I concurred!) We especially liked the
"zoo."
Several of you sent personal messages encouraging my students and
me, and also sent links and other interesting information.
Thanks again for all your help! I think I've even made a couple of
new net-friends!
Many thanks,
Julie Polak




From: Warren Straszheim :      wes-at-ameslab.gov
Date: Wed, 24 Sep 1997 09:10:20 -0500
Subject: Re: users of link izis 300

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At 10:32 AM 9/24/97 +0200, you wrote:
} I have a problem with an import the file with an extension *.sp ( spectrum )
} and put it to the document in word 6 or amipro
}
} Malgorzata Warmuzek

As a rule, you must have a program that supports OLE (object linking and
embedding) for the type of file that you have at hand before you can import
that file into another program. I suspect that you have no such program for
your SP files.

You will probably have to export the file to some form that can be read by
your spreadsheet or graphing program, prepare your graph there, and then
copy that graph into your word processor. I haven't worked much with
Microsoft's MSGRAPH that comes with Word, but it might do what you need in a
rudimentary sort of way without invoking a spreadsheet program.
----------------------------------------------------
Warren E. Straszheim
23 Town Engineering, or
270 Metals Development
Iowa State University
Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.marl.iastate.edu/marl/ (re: SEM)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)

electron microscopy, x-ray analysis, image analysis
computer applications
coal characterization and processing





From: Julia Polak :      jpolak-at-esu6.esu6.k12.ne.us
Date: Wed, 24 Sep 1997 12:19:15 +0000
Subject: Protist Pix reprise

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Hello Once Again from the Great Plains!

We had so much fun yesterday at the Microbe Zoo and at Kunkel's
Gallery, that I want to share our four favorite sites with the rest of
the world (as it were). I think these would be helpful to other upper
elementary/middle school teachers and students.

Again, thanks for all your help/

http://commtechlab.msu.edu/CTLProjects/dlc-me/zoo/

http://www.pbrc.hawaii.edu/~kunkel/gallery/

http://www.cellsalive.com/

http://www.ualberta.ca/~mingchen/images.htm/




From: mark_munro-at-bio-rad.com
Date: Wed, 24 Sep 97 18:13:06 -0800
Subject: Image databases/archiving

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Dear all,
does anyone know of any shareware/inexpensive image database and
archiving software.

Thanks in advance,

Mark Munro






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Wed, 24 Sep 1997 14:27:49 -0400
Subject: RE: Image databases/archiving

Contents Retrieved from Microscopy Listserver Archives
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I use Thumbs Plus (approx. $60 US if I remember correctly)
Cerious Software, Inc.
1515 Mockingbird Lande
Suite 209
Charlotte, NC 28209
http://www.cerious.com

I have no financial interest in this company other than doing my part as
a very satisfied customer.


} ------------------------------------------------
} Opinions or statements expressed herein, rational or otherwise, do not
} necessarily reflect those of my employer.
}
} Harold J. Crossman
} OSRAM SYLVANIA INC.
} Lighting Research Center
} 71 Cherry Hill Dr.
} Beverly, MA 01915
} Phone: (508) 750-1717
} E-mail: crossman-at-osi.sylvania.com
}
} Our web sites: www.sylvania.com
} www.siemens.com
} --
}
} "Crossman, Harold" {crossman-at-osi.SYLVANIA.com}
}
}
}




From: Janusz Chris Terlecki :      aas-at-pacbell.net
Date: Wed, 24 Sep 1997 11:39:39 -0700
Subject: SEM

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Dear colleagues,

We are in the process of resurrecting Cambridge 250 Mk3 SEM and we are
looking for the source of spare parts. Any feedback on the subject
will be greatly appreciated.

Thank you for your help!
Chris

-----------------------------------------------------------------
Chris Terlecki
Applied Analytical Sciences
3303 Harbor Blvd. Ste H-4
ph: 714-434-6894
fax: 714-434-0294
E-mail: aas-at-pacbell.net




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 24 Sep 1997 20:17:56 +0100 (BST)
Subject: Re: cryomicrotomy of leaf

Contents Retrieved from Microscopy Listserver Archives
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As far a s cryosections of leaves are concerned. It ain't easy because
of all the internal air spaces. Cryofracturing is no problem and with a
kittle care you can cryoplane the leaf ti get a nice smooth surface. I
think I know what you want to do Jolanta (are you still doing ion beam
microscopy ?) May be freeze substitution is the best approach. If all
else fails read my book

Best wishes

Patrick Echlin
University of Cambridge

On Mon, 22 Sep 1997, Jolanta Mesjasz-Przybylowicz
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
}
} Dear Microscopists,
}
}
} I would like to ask you for your advise on cryo-sectioning of plant
} material in possibly low temperature. We tried to section the frozen
} piece of leaf using Cryocut E Reichert, playing with different embedding
} media but the results were not satisfactory. We found difficulties in
} cutting thick sections (5-30 microns) even of very young leaf.
} Morphological structure was not well preserved.
}
} I really would appreciate your advise, suggestions, tips and
} technical tricks in this matter.
} What equipment do you use and what can you recommend?
}
} Thank you in advance for all your time and assistance
}
} With best regards
}
} Jolanta Mesjasz-Przybylowicz
} ************************************************************************
} Dr Jolanta Mesjasz-Przybylowicz
} National Accelerator Centre
} P.O. Box 72
} Faure 7131
} South Africa
} tel: 27-21-8433820
} fax: 27-21-8433543
} Internet: MESJASZ-at-nac.ac.za
} ************************************************************************
}





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Wed, 24 Sep 1997 15:45:55 -0400 (EDT)
Subject: Re: Image databases/archiving

Contents Retrieved from Microscopy Listserver Archives
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Thumbs plus by Cerius Software works very well for us as an image filing
tool. It provides a thumbnail of all images in a directory.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Wed, 24 Sep 1997 mark_munro-at-bio-rad.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
}
} Dear all,
} does anyone know of any shareware/inexpensive image database and
} archiving software.
}
} Thanks in advance,
}
} Mark Munro
}
}
}




From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Wed, 24 Sep 1997 22:50:25 +0200
Subject: Re: Image databases/archiving

Contents Retrieved from Microscopy Listserver Archives
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mark_munro-at-bio-rad.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
} Dear all,
} does anyone know of any shareware/inexpensive image database and
} archiving software.
}
} Thanks in advance,
}
} Mark Munro

Mark,

Company Cerious Software Inc. http://cerious.catalogue.com/index.html
has a great software ThumbPlus 3.0 for 60 US$.

Henrik Kaker
SEM-EDS Laboratory
Metal Ravne d.o.o.
Slovenia




From: Randy Tindall :      rtindell-at-nmsu.edu
Date: Wed, 24 Sep 1997 15:25:36 -0600
Subject: SEM/LM/EDX of tire particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listmembers,

We are looking for any information we can find on the
microscopy/identification of particles from automobile tires. Seems we
have a case where we need to try to match particles from a pair of tennis
shoes to a particular set of automobile tires(best case), or at least
identify particles as possibly coming from automobile tires (more likely).
(I'll let your imagination fill in the details on this one!)

If anybody has done anything like this, please let us know. In the
meantime, we'll be hitting the indexes/abstracts and databases.

Never a dull moment....
Randy Tindall
Electron Microscope Laboratory
Box 3EML
New Mexico State University
Las Cruces, NM 88003




From: mark_munro
Date: Wednesday, September 24, 1997 6:13PM
Subject: Image databases/archiving

Contents Retrieved from Microscopy Listserver Archives
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ThumbsPlus is very good. I've used a single site license. In my new
position, I've just gotten 5 concurrent site licenses for myself and my
team. I don't know how good their Mac version is. I believe that it is
still in beta testing. It is shareware and Cerious software has a website
where you can download the shareware version. Once you are registered,
there are some things that are available, but the unregistered version is
very good. I tried it out that way after it was suggested previously on the
Microscopy Listserver.

-Scott Walck

"The opinions expressed are those of S.D. Walck and not of PPG Industries,
Inc. nor of any PPG-associated companies."



----------
-----------------------------------------------------------------------.



Dear all,
does anyone know of any shareware/inexpensive image database and
archiving software.

Thanks in advance,

Mark Munro







From: Phyllis Davie :      pdavie-at-u.washington.edu
Date: Wed, 24 Sep 1997 17:53:53 -0700 (PDT)
Subject: Re: immunofluorescent techniques

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We do direct immunofluorescence on renal and skin biopsies. For the most
part, the specimens are received in Michels transport media (which we make
ourselves). Upon arrival, the specimens are washed in 3 changes of
transport buffer to flush out the excess salts of the michels media (3
washes, 10 minutes each, room temp). The specimen is then lightly
blotted, oriented in a cryomold, covered in OCT, and frozen. To freeze,
we cool iso-pentane (2-methylbutane) in liquid nitrogen, and lower the
cryomold into the cooled iso-pentane for a few seconds. We then attach a
chuck in the cryostat, and cut.
When the specimen is received fresh, we skip the washing part, and
proceed as above.
On the renal biopsies, we run: IgG, IgA, IgM, C3, C1q, Fibrinogen,
Kappa, Lambda, and Albumin.
On the skin biopsies, we run: IgG, IgA, IgM, C3, C1q, Fibrinogen, and
Albumin.

Hope this answers your question.

Phyllis Davie
Immunocytochemistry Laboratory
University of Washington Medical Center
pdavie-at-u.washington.edu




On Tue, 23 Sep 1997, Flores, Teresa wrote:

} Our lab is in the transition of doing immunogold tagging in HRLM-TEM.But we
} continue to do immunofluorescent techniques received in Michels transport
} media. Is there anyone out there still doing immunofluorescent techniques
} (direct or indirect immunofluorescent techniques) on renal, skin, muscle or
} nerve biopsies? If yes, is the biopsy snap-frozen in liquid nitrogen as
} soon after removal as possible (precooling a metal chuck for about a
} minute. Placing a small volume of saline or water on top of the colled
} chuck, immediately placing the renal or skin bx into the water or saline
} drop. As the water and the bx begin to freeze, the chuck is turned down to
} eliminate excess water or saline. The chuck is placed in liquid nitrogen
} for approx one minute to snap-freeze the bx or tissue. When nitrogen stops
} bubling bx is adequately frozen and bx is ready to be stored at -70oC or to
} be sectioned).
} If not possible to do technique is the tissue being storred in Michels'
} transport media also sold commercially as Zeus.
} Are the renal biopsies being tagged with IgG, IgM, IgA, C3, C1q, Kappa & Lambda?
} Skin, muscle and nerve biopsies tagged with IgG, IgM, IgA, C3 and Fibrinogen?
} Please respond. Thanx Teresa
}
}
}
}





From: A. Hermes :      hermes-at-equex.concom.com
Date: Wed, 24 Sep 1997 21:35:46 -0500
Subject: archive of this list?

Contents Retrieved from Microscopy Listserver Archives
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Hi folks,

I'm curious, is there an archive of the posts to this listserver? And is it
searchable from a web browser?

TIA,

Hermes





From: Chun Hua Kong :      kong-at-materials.unsw.edu.au
Date: Thu, 25 Sep 1997 14:40:58 +1000 (EST)
Subject: Re: users of link izis 300

Contents Retrieved from Microscopy Listserver Archives
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Malgorzata,

You can capture the spectrum with a software, such as Paint Shop
Pro we are using, and save it as TIFF or Bitmap file. If you have the
software, I can show you the details.

With the best wishes,
Charlie Kong
kong-at-t-rex.materials.unsw.edu.au


On Wed, 24 Sep 1997, Malgorzata.Warmuzek wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
}
} I have a problem with an import the file with an extension *.sp ( spectrum )
} and put it to the document in word 6 or amipro
}
} Malgorzata Warmuzek
} Foundry Research Institute
} Research Materials Department
} Structural and Physical Research Laboratory
}
} Zakopianska 73 Call +48 12 2605022 ext. 317
} 30-418 KRAKOW - POLAND Fax +48 12 2665478, +48 12 2660870
}
}
}





From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Thu, 25 Sep 1997 09:24:38 BST
Subject: Re: SEM

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Dear Chris
try
Electron Optical Services
52 Higher Road
Urmston nr Manchester UK
M41 9AP
+44 161 748 8448 Fax +44 161 746 8048

They have been extremely helpful and competent in maintaining a
Cambridge 250 for us. I'm sure they would be happy to supply spares
'over the pond'.

I have no financial interest in EOS and only speak as a satisfied past
customer.


Sincerely
+-----------------------------------------------------------------
|Dr Stephan Helfer, SSO
|Senior Mycologist - MSc Course Director
|
|Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
|Scotland UK
|
|http://www.rbge.org.uk
|
|phone: +44 (0)131 552 7171 ext 280
| or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
|fax: +44 (0)131 552 0382
+------------------------------------------------------------------




From: Gunnel.Karlsson-at-oorg2.lth.se (Gunnel Karlsson)
Date: Thu, 25 Sep 1997 11:01:11 +0200
Subject: Glow discharge

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I don't have access to a nice workshop, where they can construct a glow
discharger, where in Europe can I buy one? Or, is it out there someone, who
has an old mashine you don't need anymore?

TIA

Gunnel Karlsson

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Gunnel Karlsson E-mail Gunnel.Karlsson-at-oorg2.lth.se
Biomicroscopy Unit Tel +46 222 8229
Inorganic Chemistry 2 Fax +46 222 4012
Box 124
S-221 00 LUND, Sweden
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
This message was sent by Eudora with recycled electrons






From: Ian MacLaren :      I.MacLaren-at-BHAM.AC.UK
Date: Thu, 25 Sep 1997 11:08:50 +0100
Subject: Re: users of link izis 300

Contents Retrieved from Microscopy Listserver Archives
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Malgorzata,
There should be some function on your Link system for outputting the data
of a *.sp file onto a DOS disk. This can then be read in to Microsoft
Excel, plotted as a graph and the graph imported into MS word. We have a
different Link system to you so the details may vary on how you get the
Link system to output onto a DOS disk but I would be happy to send you a
copy of the procedure that works for us if that would help.

Yours sincerely


++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Ian MacLaren, Tel: (44) (0) 121 414 3447
IRC in Materials for FAX: (44) (0) 121 414 3441
High Performance Applications, email: I.MacLaren-at-bham.ac.uk
The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/
Birmingham B15 2TT,
England.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++






From: Jolanta Mesjasz-Przybylowicz :      mesjasz-at-srvnac1.nac.ac.za
Date: Thu, 25 Sep 1997 12:44:00 +0000
Subject: Re: cryomicrotomy of leaf

Contents Retrieved from Microscopy Listserver Archives
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Dear Patrick,

Thank you very much for your reply.

I have your book as a Bible on my desk. I remember our discussions
concerning this problem as well but I do hope that I will find
someone around the world who get closer to solve it.
May be you know or heard about such person?

In cryofracturing we will be not able to obtain one layer of cells,
that is a reason why I was trying cryo-sectioning.
Freeze-substitution can be an option, but one should be careful about
redistribution of ions which is our main worry in preparation for X-ray
microanalysis.

Best regards

Jolanta

} As far a s cryosections of leaves are concerned. It ain't easy because
} of all the internal air spaces. Cryofracturing is no problem and with a
} kittle care you can cryoplane the leaf ti get a nice smooth surface. I
} think I know what you want to do Jolanta (are you still doing ion beam
} microscopy ?) May be freeze substitution is the best approach. If all
} else fails read my book
}
} Best wishes
}
} Patrick Echlin
} University of Cambridge

************************************************************************
Dr Jolanta Mesjasz-Przybylowicz
National Accelerator Centre
P.O. Box 72
Faure 7131
South Africa
tel: 27-21-8433820
fax: 27-21-8433543
Internet: MESJASZ-at-nac.ac.za
************************************************************************




From: tflore-at-lsumc.edu (Flores, Teresa)
Date: Thu, 25 Sep 1997 06:53:22 -0500
Subject: Controls for immunofluorescense

Contents Retrieved from Microscopy Listserver Archives
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Anyone doing immunofluorescenst techniques in renal biopsies running a control?






From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 25 Sep 1997 07:47:15 -0500
Subject: Re: users of link izis 300

Contents Retrieved from Microscopy Listserver Archives
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For that matter, if you are running under Windows, you can use the
PrintScreen or Alt-PrintScreen keys to capture the screen and paste it into
Word or whereever. You can get fancier by using the Format Picture item in
Word to trim the bitmap to the area you want and to size it. Or you can get
fancier still and first paste the bitmap into a picture editor (e.g., LView
Pro or MS Imager or even MS Paint which come with Windows). Then you can
crop or annotate the image and save it to a file or copy it from there to
your word processor.

Note that the difference between PrintScreen and Alt-PrintScreen is that the
Alt form copies only the active window instead of the whole screen. Thus you
can size your spectrum (or any other application) as you want before
snapping a copy.

This may not be as nice as importing the spectrum in a form in all its
detail, but it will convey the information.

At 02:40 PM 9/25/97 +1000, you wrote:
} Malgorzata,
}
} You can capture the spectrum with a software, such as Paint Shop
} Pro we are using, and save it as TIFF or Bitmap file. If you have the
} software, I can show you the details.
}
} With the best wishes,
} Charlie Kong
} kong-at-t-rex.materials.unsw.edu.au
}
}
} On Wed, 24 Sep 1997, Malgorzata.Warmuzek wrote:
}
} } I have a problem with an import the file with an extension *.sp ( spectrum )
} } and put it to the document in word 6 or amipro
} }
} } Malgorzata Warmuzek
} } Foundry Research Institute
} } Research Materials Department
} } Structural and Physical Research Laboratory
} }
} } Zakopianska 73 Call +48 12 2605022 ext. 317
} } 30-418 KRAKOW - POLAND Fax +48 12 2665478, +48 12 2660870
----------------------------------------------------
Warren E. Straszheim
23 Town Engineering, or
270 Metals Development
Iowa State University
Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.marl.iastate.edu/marl/ (re: SEM)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)

electron microscopy, x-ray analysis, image analysis
computer applications
coal characterization and processing





From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Thu, 25 Sep 1997 08:16:18 -0500
Subject: Re: Glow discharge

Contents Retrieved from Microscopy Listserver Archives
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Gunnel

To make a glow discharge all you need is a poor vacuum ( ~ 100 mTorr)
and a high voltage power supply. You can use a vacuum bell jar with an
electrical
feedthru. Install a leak value in the system and rough pump it out, then
put in
a controlled leaked of what ever gas you want, air will work, but Argon
is nice and
be careful with Oxygen.

Arrange your electrode to be in the bell jar (insultate it up to the point
where
you want the "glow" to start.) Then slowly crank up the kV. Depending on your
geometry, gas pressure etc.. you should get something by the time you reach
~ 1 kV.

The more important question is what do you want to do with
the discharge?

Nestor
Your Friendly Neighborhood SysOp







From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 25 Sep 1997 10:40:19 -0400
Subject: RE: SEM/LM/EDX of tire particles

Contents Retrieved from Microscopy Listserver Archives
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Randy,

Have you tried the (Walter) McCrone Institute in Chicago? I don't have
the address handy, but maybe someone else does. They have worked on
everything from the Shroud of Turin to ancient paints.

------------------------------------------------
Opinions or statements expressed herein, rational or otherwise ;-) do
not necessarily reflect those of my employer.

Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-osi.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-osi.SYLVANIA.com}




From: Chism, Sharron :      SharronChism-at-hmhs.com
Date: Wed, 24 Sep 1997 09:07:00 -0500
Subject: Rinsing Grids

Contents Retrieved from Microscopy Listserver Archives
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Greetings All,
After each step of the UA stain and the LC stain, I rinse the
copper grids by immersion. I have four changes of water and "dip" the
grid about 40 times in each change. I am interested in changing that to
the procedure that rinses the grid by "flooding" it using a syringe or
whatever. Can someone share with me exactly how that is done ... what
type of water ... etc. Also ... do the sections ever wash off the grid
when rinsing that way??

Thanks in advance,
Sharron Chism HT (ASCP)
Electron Microscopy Lab
Harris Methodist Hospital
Fort Worth, Texas




From: Wolfgang Muss :      W.Muss-at-lkasbg.gv.at
Date: Thu, 25 Sep 1997 18:01:17 +-200
Subject: Casting your own SiRubber Moulds, 10/28/96

Contents Retrieved from Microscopy Listserver Archives
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09/25/97
Hi everywhere/everyone,
as a brand-new member of this listservice I want to add a notice on =
self-fabrication of silicone rubber molds for epoxide-resin-embedding, =
which Lonie Kerr asked for 10/28/96 (only for the case, someone has =
similar problems now).
There are articles (in English) on self fabrication of moulds for =
epoxide-embedding, at least 2 of them are:
1) MUSS W., SIMONSBERGER P. (1983): Home-made Silicone Rubber Embedding =
moulds for Electron Microscopy, Mikroskopie (Vienna) 40, 207-209
(simple version for the unexperienced)
2) MUSS W.(1984): Self fabrication of Silicone Rubber embedding Moulds =
for use in Electron Microscopy; Mikroskopie (Vienna) 41, 34-39
(sophisticated version for the unexperienced including silicone rubber =
material to be used).
I am producing all my moulds (types as shown in article 2) like cubic, =
flat, "beem capsule"- type w/round or pyramidal block face, with =
engraved numbers for identification and others as requested) by myself. =
They are needed in our routine diagnostic EM-Lab and fabricated since =
1984 without problems, with high quality in shearing force and =
unmoulding properties as well as very low costs (quantity needed a year =
for about 1300 to 1600 specimen blocks ~ 5 moulds 48 specimen =
cabinets/each; costs/mould ~ US-Dollars 5-15.-, depending on amount of =
silicone rubber mass needed).
Fabrication is simple, if negative moulds of sufficient quality are at =
hand and several general rules in working up the silicone mass are =
strictly followed.=20
Silicone rubber moulds of this type are o.k. for use with epoxide =
resins, use for hydrophilic resins like LR White, Lowicryls not tested =
yet.
If interested in how to produce such moulds efficiently and interested =
in which kind/quality of silicone rubber one should use, please send =
e-mail request to:

Wolfgang MUSS PhD
Head of EM-Lab at Pathology Department LKA
Muellner Hauptstrasse 48
A-5020 SALZBURG/AUSTRIA/Europe
phone: ++43++662+4482-4720 Ext.
fax: ++43++662+4482-882 Ext (c/o W. MUSS)
e-mail: W.Muss-at-lkasbg.gv.at.

Good luck, hope this helps somebody.
END of Message, no attachment added.





From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 25 Sep 1997 09:23:13 -0700
Subject: Re: Glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I don't have access to a nice workshop, where they can construct a glow
} discharger, where in Europe can I buy one? Or, is it out there someone, who
} has an old mashine you don't need anymore?
}
} TIA
}
} Gunnel Karlsson

You don't need a workshop to make a usable system. Get a small, cheap
handheld Tesla coil of the sort used for physics demonstrations. Identify
an unused current feedthru in your vacuum evaporator, and attach a wire
fitting to it to use as a "docking port" for the Tesla coil. Discharge the
coil into the vacuum during rough (mechanical) pump. Take care to not
discharge the coil in air; the ozone produced damages the cilia in your
respiratory system.


Caroline Schooley
Educational Outreach Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 25 Sep 1997 12:34:29 -0400 (EDT)
Subject: Re: SEM/LM/EDX of tire particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Randy,
}
} We are looking for any information we can find on the
} microscopy/identification of particles from automobile tires. Seems we
} have a case where we need to try to match particles from a pair of tennis
} shoes to a particular set of automobile tires(best case), or at least
} identify particles as possibly coming from automobile tires (more likely).
} (I'll let your imagination fill in the details on this one!)
}
} If anybody has done anything like this, please let us know. In the
} meantime, we'll be hitting the indexes/abstracts and databases.
}
We have looked at some polymer blends for which the components can
be differentially stained, and I can give you the name of our user who could
possibly tell you whether this can be done for tire particles. The HVEM can
be used to look at the structures of such particles if they are some few
micrometers thick. We can also do EDX (and have done so on a few forensic
specimens).
Yours,
Bill Tivol





From: Doug Keene :      DRK-at-shcc.org
Date: Fri, 26 Sep 1997 16:01:59 -0500 (cdt)
Subject: Re: Glow discharge

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Dear Gunnel;

See the following article for an easy to build glow
discharge unit. I built a similar one several years ago
for ionizing grids, and it works very well. All you need
is a drill, a hack saw, a drill bit, and some epoxy.
You will also need a plastic desciccator, a few feet
of wire, a few machine and sheet metal screws, and
some sheet aluminum about 1/8 to 1/16 of an inch
thick. Mine is simpler than the one described in the
article, consisting of only an aluminum disk screwed
into the cut-off end of the high voltage generator, a
larger disk placed inside the desciccator (with a wire
going to an outside ground). You will also need a
mechanical vacuum pump.

Aebi U, 1987 [See Related Articles]
A glow discharge unit to render electron microscope grids and other surfaces hydrophilic.
J Electron Microsc Tech 7(1), 29-33 (1987)
----------------------
Doug Keene
DRK-at-shcc.org






From: Doug Keene :      DRK-at-shcc.org
Date: Fri, 26 Sep 1997 16:01:59 -0500 (cdt)
Subject: Re: Glow discharge

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Dear Gunnel;

See the following article for an easy to build glow
discharge unit. I built a similar one several years ago
for ionizing grids, and it works very well. All you need
is a drill, a hack saw, a drill bit, and some epoxy.
You will also need a plastic desciccator, a few feet
of wire, a few machine and sheet metal screws, and
some sheet aluminum about 1/8 to 1/16 of an inch
thick. Mine is simpler than the one described in the
article, consisting of only an aluminum disk screwed
into the cut-off end of the high voltage generator, a
larger disk placed inside the desciccator (with a wire
going to an outside ground). You will also need a
mechanical vacuum pump.

Aebi U, 1987 [See Related Articles]
A glow discharge unit to render electron microscope grids and other surfaces hydrophilic.
J Electron Microsc Tech 7(1), 29-33 (1987)
----------------------
Doug Keene
DRK-at-shcc.org






From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Thu, 25 Sep 1997 13:35:21 -0500
Subject: Re: Casting your own SiRubber Moulds, 10/28/96

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Wolfgang: I , for one, am very interested in learning more about this
technique. I started trying to do this last month without a lot of
success. I just looked for your publication but unfortunately our somewhat
mediocre library doesn't carry Mikroskopie . So it would be a great help
to me if you could outline your procedure. I am especially interested in
your formulation of silicon rubber and where you buy the components.
Thanks in advance. Tom Phillips

-------------------------------------.
}
} 09/25/97
} Hi everywhere/everyone,
} as a brand-new member of this listservice I want to add a notice on
} self-fabrication of silicone rubber molds for epoxide-resin-embedding,
} which Lonie Kerr asked for 10/28/96 (only for the case, someone has
} similar problems now).
} There are articles (in English) on self fabrication of moulds for
} epoxide-embedding, at least 2 of them are:
} 1) MUSS W., SIMONSBERGER P. (1983): Home-made Silicone Rubber Embedding
} moulds for Electron Microscopy, Mikroskopie (Vienna) 40, 207-209
} (simple version for the unexperienced)
} 2) MUSS W.(1984): Self fabrication of Silicone Rubber embedding Moulds for
} use in Electron Microscopy; Mikroskopie (Vienna) 41, 34-39
} (sophisticated version for the unexperienced including silicone rubber
} material to be used).
} I am producing all my moulds (types as shown in article 2) like cubic,
} flat, "beem capsule"- type w/round or pyramidal block face, with engraved
} numbers for identification and others as requested) by myself. They are
} needed in our routine diagnostic EM-Lab and fabricated since 1984 without
} problems, with high quality in shearing force and unmoulding properties as
} well as very low costs (quantity needed a year for about 1300 to 1600
} specimen blocks ~ 5 moulds 48 specimen cabinets/each; costs/mould ~
} US-Dollars 5-15.-, depending on amount of silicone rubber mass needed).
} Fabrication is simple, if negative moulds of sufficient quality are at
} hand and several general rules in working up the silicone mass are
} strictly followed.
} Silicone rubber moulds of this type are o.k. for use with epoxide resins,
} use for hydrophilic resins like LR White, Lowicryls not tested yet.
} If interested in how to produce such moulds efficiently and interested in
} which kind/quality of silicone rubber one should use, please send e-mail
} request to:
}
} Wolfgang MUSS PhD
} Head of EM-Lab at Pathology Department LKA
} Muellner Hauptstrasse 48
} A-5020 SALZBURG/AUSTRIA/Europe
} phone: ++43++662+4482-4720 Ext.
} fax: ++43++662+4482-882 Ext (c/o W. MUSS)
} e-mail: W.Muss-at-lkasbg.gv.at.
}
} Good luck, hope this helps somebody.
} END of Message, no attachment added.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: Ming Chen :      mingchen-at-gpu.srv.ualberta.ca
Date: Thu, 25 Sep 1997 13:55:59 -0600 (MDT)
Subject: Re: Rinsing Grids

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Hi,

I published an article entiled as "A Modified Autowasher Device for
Rapidly Washing Large Numbers of EM-grids" in Microscopy Researdh and
Technique vol 26:177-179 (1993). Here I just copy the summary part for
you:

This device consists of a siphon system and 5 or 10 grid disks, modified
from the previous mode (Chen, 1973), for large numbers of grids with
ultrathin sections. This method improves the ease of assembling grids
onto the grids disk and also requires much less stain solution. This
system only takes 5 min for one single stain washing, at a maximum of 100
grids, and also avoids stain contamination. The grid disk can also be used
for immunocytochemistry work and for critical point drying of grids with
biological specimens.

----------------------------------------------------------------------------

You may go to SPI Supplies website at
http://www.cccbi.chester.pa.us/spi/new/stanwash.html/ for details.

Good luck,

Ming



On Wed, 24 Sep 1997, Chism, Sharron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Greetings All,
} After each step of the UA stain and the LC stain, I rinse the
} copper grids by immersion. I have four changes of water and "dip" the
} grid about 40 times in each change. I am interested in changing that to
} the procedure that rinses the grid by "flooding" it using a syringe or
} whatever. Can someone share with me exactly how that is done ... what
} type of water ... etc. Also ... do the sections ever wash off the grid
} when rinsing that way??
}
} Thanks in advance,
} Sharron Chism HT (ASCP)
} Electron Microscopy Lab
} Harris Methodist Hospital
} Fort Worth, Texas
}


***********************************************
* Ming H. Chen, PhD *
* Medicine/Dentistry Electron Microscopy Unit *
* University Of Alberta. *
* Edmonton, Alberta, Canada *
* *
* Visit My Page At: *
* http://www.ualberta.ca/~mingchen *
***********************************************








From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: Thu, 25 Sep 1997 19:34:33 -0500
Subject: Re: Image databases/archiving

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Mark,
I've been using a product called imageAXS CE v3.0. This is a free version of
a slightly more flexible commercial program. It automatically finds image
files and creates a MS Access database which you can edit and use for
searching and indexing images. the url is:

http://www.dascorp.com

Hope this helps glenn



Glenn Poirier Tel (514) 398 -6774
Electron Microprobe Laboratory Fax (514) 398 4680
Earth and Planetary Sciences glennp-at-stoner.eps.mcgill.ca
McGill University
http://castaing.eps.mcgill.ca
3450 University St.
Montreal, Qc H3A 2A7

There are three sides to every story:
Your side,
My Side
and the truth






From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Thu, 25 Sep 1997 20:33:09 -0700
Subject: Re: Casting your own SiRubber Moulds, 10/28/96

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Dear Tom,
I have been casting my own silicon rubber molds for some time now. These are
one-inch molds for epoxy metallurgical mounts. The silicon rubber comes from
Dow Corning and they have several strengths and hardnesses. First, I have a kit
consisting of several 100 ml tri-pour (plastic) beakers with a hole drilled
in the
bottom to fit a short screw. A block of aluminum, one inch in diameter and
about 3/4 inch high, is tapped to receive the screw. You screw the aluminum
block securely into the bottom of the beaker, mix the SiRubber (according to
the directions) in a disposable cup, outgas the SiRubber in a vacuum desicator,
then pour the rubber into the beaker. Outgas again. Leave it overnight to set,
then remove the set rubber in the morning. It is a bit of a struggle to remove:
I take out the screw, then push up on the Al block with a sharp point. Any
flaps can be cut off with a razor blade. You can cast these things in any shape
you can imagine.

You wrote:
} Wolfgang: I , for one, am very interested in learning more about this
} technique. I started trying to do this last month without a lot of
} success. I just looked for your publication but unfortunately our somewhat
} mediocre library doesn't carry Mikroskopie . So it would be a great help
} to me if you could outline your procedure. I am especially interested in
} your formulation of silicon rubber and where you buy the components.
} Thanks in advance. Tom Phillips
}
} -------------------------------------.
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: mskittee-at-swbell.net
Date: Fri, 26 Sep 1997 00:52:51 -0500
Subject: SEM photography

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What are some of the problems associated with making a photograph of a
single atom? and What methods are used for getting around this problem?
please send response to :

mskittee-at-swbell.net




From: Richard Lander :      richard.lander-at-stonebow.otago.ac.nz
Date: Fri, 26 Sep 1997 18:14:43 +1200
Subject: TEM: Potassium Ferocyanide and OsO4

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Dear all,

A quick query...

We currently use Potssium ferrocyanide reduced OsO4 as our 'routine' post
fixative, primarily for enhanced membrane staining. When it works, tissue
stains up beautifully!
However, every now and then, we get tissue which appears to not have
infiltrated properly ater using this post fixative. Nine times out of ten,
it is a result of the potassium ferrocyanide (as repeat tests with new/no
Pot Ferro show).
According to the literature, (I think even to the original pot ferro paper)
they do mention that extra care must be taken when infiltrating into epoxy
resin when using this stuff.
It only seems to be intermittent, even with fresh Pot ferro made up.

Does anybody know what is the pot ferro stock solution shelf life is? (we
use stuff between 4 and 8 weeks old after 'brewing' for the first four)
What does the pot ferro react with in the tissue to prevent good infiltration?
Any other ideas/suggestions?


I'd appreciate any help on this,

Thanks

Rich.

P.S. Thanks for all the replies to an earlier request (4 - 5 weeks!) for
info re: charging policies for EM Units too! :-)

-----------------------------------------------------------------------
Richard Lander
Electron Microscope Technician
South Campus Electron Microscope Unit
Otago School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand.
Tel. National 03 479 7301 Fax. National 03 479 7254

"Southernmost EM Unit in the World!"
------------------------------------------------------------------------






From: alexander.black-at-ucg.ie (Alex Black)
Date: Fri, 26 Sep 1997 10:55:56 +0000
Subject: TEM: Potassium Ferocyanide and OsO4

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Hello all,
Has anyone been successful in labelling Substance-p for TEM? I
am trying to tag it in endothelial cells, and have been having as much
luck as a snowball would have in hell. Any help would be appreciated,
as the old PhD may hinge on this some day!
While I am here, if anyone has used DiI
(3,3'-dioctadecyloxacarbocyanine percholate) or DiO
(1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine percholate)
for neuronal tract tracing, please email me?
......................................................................
.......................................
Alex Black
Department of Anatomy
National University of Ireland
Galway

alexander.black-at-ucg.ie


Alex Black BSc, MMedSc
Department of Anatomy
National University of Ireland, Galway
Galway, Ireland






From: Karlene Hewan-Lowe :      khewanl-at-emory.edu
Date: Fri, 26 Sep 1997 07:08:24 -0400 (EDT)
Subject: Controls for immunofluorescense

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RENAL BIOPSY - IMMUNOFLUORESCENCE CONTROLS
} Anyone doing immunofluorescenst techniques in renal biopsies running a control?
}
No.

One reason is that there is not enough tissue to go around. It is very
difficult to get control sections from a positive case and there are not
enough positive cases that are archived to pproduce the material.

Any suggestions?
|--------------------------------|
| Karlene Hewan-Lowe, M.B., B.S. |
| Department of Pathology |
| Emory University |
| Phone: 404-686-2926 |
| Fax: 404-6864978 |
|--------------------------------|





From: Wolfgang Muss :      W.Muss-at-lkasbg.gv.at
Date: Fri, 26 Sep 1997 13:32:31 +-200
Subject: Casting your own Silicone Rubber molds 09/26/97

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Dear colleagues,
thank you for replies to my topic from yesterday and your interest. =
Lucky to see that there are some out there in need for such a procedure. =
God bless you!
To handle the problem via e-mail I think would go to far, concerning =
spaceneeds of informations. Unfortunately I do have neither a homepage =
(for the future there is planned one) nor a scanner unit, so I am not =
able to reproduce those papers for you by e-mail. So I shall send ASAP =
to all of you (see below) written infos to you.
Unfortunately I don=B4t have original reprints of that papers mentioned =
in my information.So you will get copies, as an attachment I shall send =
Instructions which summarize the most important things in my opinion to =
do the job as optimal as possible.=20
So my message to all who responded till now and others maybe following:
Thank you all very much for your interest/greetings (Keith! welcome =
greetings too!):
- Jerzy BOHDANOWICZ, GDANSK/Poland,
- Julian P.S. SMITH III, ROCK-HILL, SC/USA
- Phil OSHEL, CHAMPAIGN, IL/USA (will contact you separately with =
respect to "Microscopy today")
- Tom PHILLIPS, COLUMBIA, MO/USA
- Ann Fook YANG, OTTAWA, ONT/CANADA
- Scott WHITTAKER, GAINESVILLE, FL/USA
- Gabriel Adriano ROSA, BUENOS AIRES/ARGENTINA=20
- John J. BOZZOLA, CARBONDALE, IL/USA
you will get written/copied information ASAP.
Sincerely yours
Wolfgang MUSS
Dept. Pathology LKA (Gen.County Hospital), EM-Lab, Muellner Hauptstrasse =
48,
A-5020 SALZBURG/AUSTRIA-Europe, phone: ++43++662+4482+4720 Ext, =
Fax:++43++662+4482+882 Ext ("c/o W.MUSS")
End of message, no attachment added.






From: Wolfgang Muss :      W.Muss-at-lkasbg.gv.at
Date: Fri, 26 Sep 1997 14:15:33 +-200
Subject: Rinsing grids

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Re to Sharron CHISM, Fort Worth Tx; 09/26/97
Dear Sharron,
whatever type of aid (apparatus) you will use for staining your =
ultrathins:
concerning water quality: if possible, use UHQ (ultrahigh-purified =
water) or at least bi- (better triple-)distilled water from a =
quartz-glass distilling apparatus (most conveniently in your own lab!). =
My/our story:
our quartz-glass bi-distilling apparatus which at that time had a =
life-span of nearly 12 years (in which time we got no bigger problems at =
all) had broken down (heating wire burned through). Therefore we thought =
to overcome the problem in ordering } } A.bidest.sterile, "non pyrogenic" =
{ { via our hospital pharmacy (+/- every time freshly produced, etc.). In =
fact we got worsest stainings of our sections due to precipitates never =
seen before in such an amount and shapes. We thought this to be a =
possible source with respect to our handling in washing the grids, =
unclean glass ware, syringes, etc.... or just more dust in the lab or =
else; nothing of that all: despite using same methods for mixing up our =
staining solutions as usual, we were not able to locate this source of =
junk on the grids! When I asked at our pharmacy, how they produce their =
water, they showed me & told me about their apparatus: it was/is a still =
producing vapours by means of copper plates! An analytic chemist told me =
some days after, that their central analytic lab got their own =
distilling (UHQ) machine because of too high copper-contents of the =
bidistilled water of the former source. Since that info I used only =
their UHQ (coming along in clean glassware, most preferably quartz =
glass) with no precipitate-problems any more, hoping to get my old =
quartzglass still as soon as possible from repairing.
Hope this adds another aspect in "hunting our elephantine precipitates",
Best regards
Wolfgang MUSS
SALZBURG/Austria, Europe




From: tflore-at-lsumc.edu (Flores, Teresa)
Date: Fri, 26 Sep 1997 07:39:22 -0500
Subject: Re: Rinsing Grids

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Sharron, I also stain with Uranyl Acetate 5 min and Lead Citrate 5 min. I
place copper grids, section side down, on drops of UA and LC. Inbetween
stain and after I grasp edge of copper grid and flood by dripping from top
of forcep for approx 10 sec each grid. I use Distilled, Sterile water to
rinse. any reason for change. I know several techs that rinse as you do,
but felt there needed to be changed. I've been staining as above for over
22 years.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Fri, 26 Sep 1997 08:44:14 -0600
Subject: water contamination/rinsing grids

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I would like to point out another source of contamination in stills: the
tubing. Not the obvious crud, but breakdown products. We were using
manufacturer-recommended Tygon tubing on our Barnsted still, and we getting
some mysterious scum in the distillate. The micro-analytical lab couldn't
identify it, other than as some complex organic kind of thing. Process of
elimination ended at the tubing. The distillate was coming off at below,
but not much below, the breakdown temperature of the tubing. The tubing was
changed for higher-temperature rated silicone tubing. This seemed to solve
the "new contamination" problem, but the previous contamination had coated
the inside of the glass (not sure if it was quartz) carbouy and wouldn't
clean off (not even will Tilex Scum Remover).

Phil
}
} Re to Sharron CHISM, Fort Worth Tx; 09/26/97
} Dear Sharron,
} whatever type of aid (apparatus) you will use for staining your ultrathins:
} concerning water quality: if possible, use UHQ (ultrahigh-purified water)
} or at least bi- (better triple-)distilled water from a quartz-glass
} distilling apparatus (most conveniently in your own lab!). My/our story:
} our quartz-glass bi-distilling apparatus which at that time had a
} life-span of nearly 12 years (in which time we got no bigger problems at
} all) had broken down (heating wire burned through). Therefore we thought
} to overcome the problem in ordering } } A.bidest.sterile, "non pyrogenic" { {
} via our hospital pharmacy (+/- every time freshly produced, etc.). In fact
} we got worsest stainings of our sections due to precipitates never seen
} before in such an amount and shapes. We thought this to be a possible
} source with respect to our handling in washing the grids, unclean glass
} ware, syringes, etc.... or just more dust in the lab or else; nothing of
} that all: despite using same methods for mixing up our staining solutions
} as usual, we were not able to locate this source of junk on the grids!
} When I asked at our pharmacy, how they produce their water, they showed me
} & told me about their apparatus: it was/is a still producing vapours by
} means of copper plates! An analytic chemist told me some days after, that
} their central analytic lab got their own distilling (UHQ) machine because
} of too high copper-contents of the bidistilled water of the former source.
} Since that info I used only their UHQ (coming along in clean glassware,
} most preferably quartz glass) with no precipitate-problems any more,
} hoping to get my old quartzglass still as soon as possible from repairing.
} Hope this adds another aspect in "hunting our elephantine precipitates",
} Best regards
} Wolfgang MUSS
} SALZBURG/Austria, Europe

}}}}}}}}}}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{{{{{{{{{{

Philip Oshel
Station A
PO Box 5037
Champaign, IL 61825-5037
(217) 355-1143
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again *****







From: Ronald M. Anderson (1-914-892-2225) :      ron-anderson-at-vnet.ibm.com
Date: Fri, 26 Sep 97 09:01:57 EDT
Subject: Award Nomination

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MSA has invited 1998 award nominations.

Among the awards to be presented next year is the
"Morton D. Maser Distinguished Service Award," to be
presented to an individual to ..."recognize outstanding volunteer service
to the Society, ... and who has served the Society for many years
with great dedication."

The nomination is to include a letter (this e-mail) from the primary
nominator and supplemental letters (e-mails) of support from others.

It is with great personal pleasure that I nominate Nestor Zaluzec
for this award.

Nestor is our friendly SYSOP, has organized the Argonne e.m. computer
resources on behalf of microscopists everywhere, has organized the
computer workshop/software exchanges at our meetings, served as
MSA Program Chair for the Minneapolis meeting, etc., etc.

If you would like to provide a supplemental e-mail of support
please direct your contribution to Gracie Burke, MSA Awards Committee.
Deadline for input is December 31, 1997.

mgburke-at-pitt.edu

Thank you.

Ron Anderson


p.s. Please DO NOT send or copy your letters to the listserver, it's bad
enough that Gracie will never speak to me again!




From: Ann-Fook Yang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Fri, 26 Sep 1997 10:09:15 -0400
Subject: Balzers parts

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Where can I get parts for my Balzers freeze-etching
equipment?


Ann Fook Yang
EM Unit,
ECORC,
Agriculture and Agri-Food Canada,
Central Experimental Farm,
Ottawa, Ontario,
Canada K1A 0C6

Tel:+1-613-759-1638
Fax: +1-613-759-1701
e-mail: yanga-at-em.agr.ca




From: rschoonh-at-sph.unc.edu (Robert Schoonhoven)
Date: Fri, 26 Sep 1997 10:22:42 +0000 (est)
Subject: Re: shelf life of Paraplast?

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Gary,

It is very possible that the wax has gone "bad".

What is probably happening is that the paraffin wax is seperating from the monomers that have been
added to it. This can happen under several circunstances. The most common being that the
temperature on the paraffin dispencer is set too high for the wax being used. Another reason could
be that the wax has been sittting unused (but hot) for too long a time (several weeks or more).

-- Begin original message --

} From: Gary Radice {gradice-at-richmond.edu}

}
} I keep Paraplast Plus melted in a bath more or less continuously, so it is
} always ready to use, even though it may be one or two months between uses.
} Lately my students have been having some sectioning problems (with freshly
} sharpened blades) and before I suggest that maybe they have done something
} wrong during embedding, is it possible that the wax has "gone bad?"
}
} Gary Radice, Associate Professor gradice-at-richmond.edu
} Department of Biology 804-289-8107 (voice)
} University of Richmond VA 23173 804-289-8233 (FAX)
-- End original message --


regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123





From: Chism, Sharron :      SharronChism-at-hmhs.com
Date: Fri, 26 Sep 1997 10:42:00 -0500
Subject: Thanks Everyone!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you to all who responded to my questions about rinsing grids via
"flooding". I had quite a few responses and I'm going to give it a try!

Sharron G. Chism HT (ASCP)
Electron Microscopy Lab
Harris Methodist Hospital
Fort Worth, Texas




From: Ziel Rainer :      Rainer.R.Ziel-at-Obernburg.ARLO.akzo.nl (Tel 49\(0\)6022-812645)
Date: Fri, 26 Sep 1997 20:51:28 +0200
Subject: RE: users of link izis 300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} } Malgorzata Warmuzek wrote:
} } I have a problem with an import the file with an extension *.sp ( spectrum )
and put it to the } } document in word 6 or amipro

Dear Malgorzata,

I use the ISIS suite 3.0 and there is an easy way to copy spectra to any other
windows program, maybe it works also with your version. Use the command
'Buttons' 'Print' and a new window will appear called 'Spectrum printing'.
There it is possible to activate the command 'Edit' 'Copy'. The Spectrum will
now be transfered to the clipboard in form of a vector graphic.

Kind regards

Rainer
-------------------------------------------
Rainer Ziel
Akzo Nobel Central Reasearch
63784 Obernburg - Germany




From: edelmare-at-casmail.muohio.edu
Date: Fri, 26 Sep 1997 13:02:00 -0500
Subject: Re: Balzers parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


North American Balzers rep is:

Technotrade International
7 Perimeter Road
Manchester, NH 03103-3343
Tel (603)622-5011
Fax (603)622-5211

I've found them to be very pleasent, helpful and friendly to deal
with. Alternatively you could contact Balzers directly

Bal-Tec AG
Postfach 75
FL-9496 Balzers
Furstentum Leichtenstein
TEL +75/388 56 11
fax +75/388 56 60


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu


"640K ought to be enough for anybody."
-- Bill Gates, 1981




From: Lesley Weston :      lesley-at-unixg.ubc.ca
Date: Fri, 26 Sep 1997 10:11:28 -0700 (PDT)
Subject: Re: Casting your own SiRubber Moulds, 10/28/96

Contents Retrieved from Microscopy Listserver Archives
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It's possible to make excellent moulds from the material that dentists use
to make dental impressions. This is usually polyvinyl siloxane, though
there is also a polyether material that is more awkward to work with. The
polyviynl siloxane (Perfourm, Reprosil etc.) comes in tubes (cheaper) or
in cartridges which give a much better result. We have been using these
materials in our lab for many years. They are available from any dental
supplier.
Hope this helps.
Lesley Weston.


On Thu, 25 Sep 1997, Wolfgang Muss wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} 09/25/97
} Hi everywhere/everyone,
} as a brand-new member of this listservice I want to add a notice on self-fabrication of silicone rubber molds for epoxide-resin-embedding, which Lonie Kerr asked for 10/28/96 (only for the case, someone has similar problems now).
} There are articles (in English) on self fabrication of moulds for epoxide-embedding, at least 2 of them are:
} 1) MUSS W., SIMONSBERGER P. (1983): Home-made Silicone Rubber Embedding moulds for Electron Microscopy, Mikroskopie (Vienna) 40, 207-209
} (simple version for the unexperienced)
} 2) MUSS W.(1984): Self fabrication of Silicone Rubber embedding Moulds for use in Electron Microscopy; Mikroskopie (Vienna) 41, 34-39
} (sophisticated version for the unexperienced including silicone rubber material to be used).
} I am producing all my moulds (types as shown in article 2) like cubic, flat, "beem capsule"- type w/round or pyramidal block face, with engraved numbers for identification and others as requested) by myself. They are needed in our routine diagnostic EM-
Lab and fabricated since 1984 without problems, with high quality in shearing force and unmoulding properties as well as very low costs (quantity needed a year for about 1300 to 1600 specimen blocks ~ 5 moulds 48 specimen cabinets/each; costs/mould ~ US-
Dollars 5-15.-, depending on amount of silicone rubber mass needed).
} Fabrication is simple, if negative moulds of sufficient quality are at hand and several general rules in working up the silicone mass are strictly followed.
} Silicone rubber moulds of this type are o.k. for use with epoxide resins, use for hydrophilic resins like LR White, Lowicryls not tested yet.
} If interested in how to produce such moulds efficiently and interested in which kind/quality of silicone rubber one should use, please send e-mail request to:
}
} Wolfgang MUSS PhD
} Head of EM-Lab at Pathology Department LKA
} Muellner Hauptstrasse 48
} A-5020 SALZBURG/AUSTRIA/Europe
} phone: ++43++662+4482-4720 Ext.
} fax: ++43++662+4482-882 Ext (c/o W. MUSS)
} e-mail: W.Muss-at-lkasbg.gv.at.
}
} Good luck, hope this helps somebody.
} END of Message, no attachment added.
}
}





From: Ya Chen :      ychen14-at-facstaff.wisc.edu
Date: Fri, 26 Sep 1997 13:12:53 -0600
Subject: Re: Balzers parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You can contact:

Technotrade International
7 Perimeter Rd
Manchester, NH 03103-3343
Tel: 603-622-5011
FAX: 603-622-5211

Ya Chen




Ya Chen

========================================================================
\ / Integrated Microscopy Resource (IMR)--
\ / __ an NIH Biomedical Research Resource TEL: 608-263-8481
\/ / / University of Wisconsin-Madison FAX: 608-265-4076
/ / / 1675 Observatory Drive #159
/ /__/_ Madison, WI 53706 Email: ychen14-at-facstaff.wisc.edu
========================================================================
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html






From: Dr. David Hall :      hall-at-aecom.yu.edu
Date: Fri, 26 Sep 1997 15:16:43 -0400
Subject: frozen thin sections of isolated cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a problem obtaining frozen ultra-thin sections of cultured cells.
We have tried both 2.3 M sucrose and 20% PVP/1.8 M sucrose to infiltrate
the cells before freezing without success. Briefly, after fixing the cells
and rinsing with buffer, the plates are scraped and the cells are
microfuged briefly to pellet the cells. Then we add a small amount
(approximately 1X the volume of the cell pellet) of sucrose or PVP/sucrose
to the pellet of cells, and tap the tube to resuspend the cells. The next
day a small amount of the cell suspension is placed on a metal peg and
frozen in liquid nitrogen. When sectioning we obtain snow-like flakes on
the knife edge. Tissues processed in a similar manner cut well. We
suspect the problem to be too much buffer associated with the cell pellet,
even though we try to remove as much buffer from the pellet as possible.
Any suggestions would be appreciated.

Christine Roy and David Hall
Albert Einstein College of Medicine
Bronx, NY




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 26 Sep 1997 20:35:58 +0100
Subject: Re: SEM photography

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Considerable! If you or anybody else can image a single atom in an SEM,
then you're edging into Nobel Prize territory (although it's relatively
easy in a dedicated STEM). Under the correct circumstances, it's not too
difficult to do it in a TEM either. For example, isolated uranium atoms on
a thin carbon film support are relatively easy. If you can manage a single
sulphur atom on a carbon support in a SEM, then I guess you'll be off to
Sweden. On the other hand, there are a number of 'probe' instruments - STM,
AFM, etc - around which can visualise individual atoms without any
difficulty. However, if you want to understand what the image really means,
then it starts to get difficult:)

This seems to me to be another case of where the real answer to the
question is a visit to your local library. I know they aren't necessarily
'high-tech', but there still isn't much around electronicaly to beat a
visit to a good library, sitting down, going through the journals, indexes,
citation catalogues, etc. It takes time but it will give you what you want
- refereed papers in reputable journals.

Regards,
Larry Stoter






From: MIKE ROCK :      merock-at-du.edu
Date: Fri, 26 Sep 1997 13:39:19 -0600 (MDT)
Subject: Re: Rinsing Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sharron,
if you don't like the dip method try the dilution method, because any
flooding method with the syringe or whatever may destroy your sections,
try allowing the grid to float to the bottom of a small beaker (10-20 ml)
repeat 3-5 times, pour off the excess water to retrieve the grid, and
rember to pick it up by the edge of the grid.
-MR

On Wed, 24 Sep 1997, Chism, Sharron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Greetings All,
} After each step of the UA stain and the LC stain, I rinse the
} copper grids by immersion. I have four changes of water and "dip" the
} grid about 40 times in each change. I am interested in changing that to
} the procedure that rinses the grid by "flooding" it using a syringe or
} whatever. Can someone share with me exactly how that is done ... what
} type of water ... etc. Also ... do the sections ever wash off the grid
} when rinsing that way??
}
} Thanks in advance,
} Sharron Chism HT (ASCP)
} Electron Microscopy Lab
} Harris Methodist Hospital
} Fort Worth, Texas
}




From: MIKE ROCK :      merock-at-du.edu
Date: Fri, 26 Sep 1997 13:39:19 -0600 (MDT)
Subject: Re: Rinsing Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sharron,
if you don't like the dip method try the dilution method, because any
flooding method with the syringe or whatever may destroy your sections,
try allowing the grid to float to the bottom of a small beaker (10-20 ml)
repeat 3-5 times, pour off the excess water to retrieve the grid, and
rember to pick it up by the edge of the grid.
-MR

On Wed, 24 Sep 1997, Chism, Sharron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Greetings All,
} After each step of the UA stain and the LC stain, I rinse the
} copper grids by immersion. I have four changes of water and "dip" the
} grid about 40 times in each change. I am interested in changing that to
} the procedure that rinses the grid by "flooding" it using a syringe or
} whatever. Can someone share with me exactly how that is done ... what
} type of water ... etc. Also ... do the sections ever wash off the grid
} when rinsing that way??
}
} Thanks in advance,
} Sharron Chism HT (ASCP)
} Electron Microscopy Lab
} Harris Methodist Hospital
} Fort Worth, Texas
}




From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 26 Sep 1997 11:53:22 -1000 (HST)
Subject: old Denton freeze etch unit available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello, all-
Are any of you interested in a Denton DFE-3 Freeze Etch Unit that fits on
a Denton DV-502 vacuum evaporator? Please don't respond unless you are
reasonably sure you know what this is, and think you might want it,
anyway! It seems to be in excellent condition.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 26 Sep 97 19:08:00 PDT
Subject: TEM of nonconductors-carbon coat both side?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am finally starting to prepare some TEM samples of glass. Does anyone
know whether a light coat of carbon should be applied to both sides of the
sample or is just one side (I assume the top side in the microscope) good
enough?

-Scott

Scott D. Walck
PPG Industries, Inc.
Guys Run Rd. (packages)
P.O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of S.D. Walck and not of PPG Industries,
Inc. nor of any PPG-associated companies."




From: Jill Craig :      jcraig-at-unbc.ca
Date: Fri, 26 Sep 1997 16:13:59 -0700 (PDT)
Subject: fungal hyphae - critical point dry/freeze dry

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I have been asked to prepare some delicate fungal hyphae
samples. I have a Denton critical point dryer and a freeze
dryer. I'm more comfortable using the freeze dryer. Has
anyone used a freeze drying technique on similar samples? Or
does anyone in Alberta or British Columbia have a Denton
critical point dryer that I might be able to come and see how
to properly use. I'm not convinced that I'm using this one
properly. Any suggestions appreciated.


Thanks,


Jill Craig






From: RCHIOVETTI-at-aol.com
Date: Fri, 26 Sep 1997 23:48:22 -0400 (EDT)
Subject: Re: frozen thin sections of isolated cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Christine and David,

It is indeed possible there may be excess buffer with the cells. Do you get
*anything* in the way of sections that you can observe in the scope? If so,
what do you see?

1. Do the fixed cells stay nicely as a pellet? After you fix and wash the
cells, perhaps you could transitionally embed the pellet in low-melt agarose
or 1.5% agar to hold the cells together. Do this only if the fixed pellet
does not hold together on its own. Some fixed cells will form a nice pellet
and stay that way. Others tend to go back into suspension.

2. *Definitely* increase the amount of cryoprotectant, well in excess of the
volume of the pelletted cells. Try a trick which we used to use for some
tissues: Assuming you have maybe a 50-100 microliter packed cell volume,
fill a small 1-2 ml centrifuge tube, Eppendorf (TM) or similar, with the
cryoprotectant. Then gently layer the cell pellet, embedded if necessary as
in #1, above, on top of the cryoprotectant and very gently push the pellet
*just under* the surface of the cryoprotectant. Allow the pellet to sink to
the bottom of the tube on its own accord. This may take overnight.
Infiltration with the cryoprotectant is complete when the pellet reaches the
bottom of the tube. There is usually no need to leave the pellet in the
cryoprotectant for longer periods of time. You can remove it and mount on a
pin immediately after it reaches the bottom of the tube.

Good Luck! Let us know the secret after you get the problem solved.

Best regards,

Bob Chiovetti




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Sat, 27 Sep 1997 12:18:24 +0200 (MET DST)
Subject: Re: TEM of nonconductors-carbon coat both side?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is a multi-part message in MIME format.

------=_NextPart_000_01BCCADB.8B1B4500
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 7bit

Imaging atoms? Carefully consider the affects of potential probe size on
the image, electron beam(and descrete potential electron optic
subcomponents) and AFM tip size. SPM(AFM) and EM may be producing images
heavily convoluted by tip or e-beam probe size contour. Atoms or multiple
probe images?


----------
} From: Larry Stoter {LPS-at-teknesis.demon.co.uk}
} To: mskittee-at-swbell.net; Microscopy-at-sparc5.microscopy.com
} Subject: Re: SEM photography
} Date: Friday, September 26, 1997 12:35 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } -----------------------------------------------------------------------.
} }
} } What are some of the problems associated with making a photograph of a
} } single atom? and What methods are used for getting around this problem?
} } please send response to :
} }
} } mskittee-at-swbell.net
}
} Considerable! If you or anybody else can image a single atom in an SEM,
} then you're edging into Nobel Prize territory (although it's relatively
} easy in a dedicated STEM). Under the correct circumstances, it's not too
} difficult to do it in a TEM either. For example, isolated uranium atoms
on
} a thin carbon film support are relatively easy. If you can manage a
single
} sulphur atom on a carbon support in a SEM, then I guess you'll be off to
} Sweden. On the other hand, there are a number of 'probe' instruments -
STM,
} AFM, etc - around which can visualise individual atoms without any
} difficulty. However, if you want to understand what the image really
means,
} then it starts to get difficult:)
}
} This seems to me to be another case of where the real answer to the
} question is a visit to your local library. I know they aren't necessarily
} 'high-tech', but there still isn't much around electronicaly to beat a
} visit to a good library, sitting down, going through the journals,
indexes,
} citation catalogues, etc. It takes time but it will give you what you
want
} - refereed papers in reputable journals.
}
} Regards,
} Larry Stoter
}
}
------=_NextPart_000_01BCCADB.8B1B4500
Content-Type: text/html; charset=ISO-8859-1
Content-Transfer-Encoding: quoted-printable

{html} {head} {/head} {BODY bgcolor=3D"#FFFFFF"} {p} {font size=3D2 =
color=3D"#000000" face=3D"Arial"} Imaging atoms? Carefully consider the =
affects of potential probe size on the image, electron beam(and descrete =
potential electron optic subcomponents) and AFM tip size.  SPM(AFM) =
and  EM may be producing images heavily convoluted by tip or e-beam =
probe size contour. Atoms or multiple probe images? {br} {font size=3D2 =
color=3D"#008080"} {br} {br} {font color=3D"#000000"} ---------- {br} > =


}
} I am finally starting to prepare some TEM samples of glass. Does anyone
} know whether a light coat of carbon should be applied to both sides of the
} sample or is just one side (I assume the top side in the microscope) good
} enough?
} -Scott

One side carbon coating is sufficient. It can be on the top or the down
side in the microscope, though you should get a better image if you place
it on top, actually. If you do not coat, be sure you will charge and
explode the sample immediately.

Yves Maniette





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Sat, 27 Sep 1997 15:09:45 -0400 (EDT)
Subject: Re: frozen thin sections of isolated cells

Contents Retrieved from Microscopy Listserver Archives
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On Fri, 26 Sep 1997, Dr. David Hall wrote:

} Date: Fri, 26 Sep 1997 15:16:43 -0400
} From: Dr. David Hall {hall-at-aecom.yu.edu}
} To: Microscopy Forum {microscopy-at-sparc5.microscopy.com}
} Subject: frozen thin sections of isolated cells
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} We have a problem obtaining frozen ultra-thin sections of cultured cells.
} We have tried both 2.3 M sucrose and 20% PVP/1.8 M sucrose to infiltrate
} the cells before freezing without success. Briefly, after fixing the cells
} and rinsing with buffer, the plates are scraped and the cells are
} microfuged briefly to pellet the cells. Then we add a small amount
} (approximately 1X the volume of the cell pellet) of sucrose or PVP/sucrose
} to the pellet of cells, and tap the tube to resuspend the cells. The next
} day a small amount of the cell suspension is placed on a metal peg and
} frozen in liquid nitrogen. When sectioning we obtain snow-like flakes on
} the knife edge. Tissues processed in a similar manner cut well. We
} suspect the problem to be too much buffer associated with the cell pellet,
} even though we try to remove as much buffer from the pellet as possible.
} Any suggestions would be appreciated.
}
} Christine Roy and David Hall
} Albert Einstein College of Medicine
} Bronx, NY
}
Sounds to me as if your sections are sucrose (the "snow" or white
stuff). I suspect your cells are thinly dispersed in the sucrose, and
not stuck together enough to make a "section."

We add paraformaldehyde to the monolayers, swirl for just a few min (~5),
scrape with a rubber policeman and pellet into a small tipped tube:

| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
\ /
H
U almost exactly this size.

We pellet in a swinging bucket centrifuge and then microfuge to pack
the cells. We let them fix for another hour or two and cut off the very
bottom and again just above the cells, forming a log with the cells in
the center that can be pushed out with a paper clip. If they stick
together, fine, proceed. If not, push them into small piles (~0.5-1 mm),
on a piece of Parafilm, drain them with filter paper cut into pie-shaped
wedges using the
very tip to touch the pellet gently, and coat them with cooled, still
molter 1% agar. Cut away any excess agar. Inflitrate with 3 changes
of sucrose (2.3M) over about 30-60 min. Place onto stubs and flash
freeze. This keeps the cells together, not dispersed thinly in your
sucrose.

If you fix very long before pelleting, your cells will not like to stick
together, and will disperse in the sucrose. The consistency of the cell
pellet should be like cooked oatmeal. (I could make some "snotty" comment
about consistencies of other substances). If they are too wet, they will
disperse, and you'll have to hunt all over your grid for them. If
they're too dry, you could alter the ultrastructure.

Good luck.
S



Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: dmatthew-at-providence.edu () (by way of Nestor J. Zaluzec)
Date: Sun, 28 Sep 1997 09:07:24 -0500
Subject: Millonig's phosphate buffer?

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(dmatthew-at-providence.edu) on Saturday, September 27, 1997 at 16:01:50
---------------------------------------------------------------------------

Email: dmatthew-at-providence.edu
Name: Douglas Matthews

School: Providence College

State: RI
Question: Can anyone help me find a recipe for Millonig's phosphate buffer?


---------------------------------------------------------------------------






From: m g burke :      mgburke-at-vms.cis.pitt.edu
Date: Sun, 28 Sep 1997 20:48:57 -0500 (EST)
Subject: 1998 Microscopy Society of America Awards Program

Contents Retrieved from Microscopy Listserver Archives
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1998 MICROSCOPY SOCIETY OF AMERICA AWARDS

All MSA Members are reminded that nominations are currently being solicited
for the 1998 MSA Awards. The Awards include:

MSA Distinguished Scientist (Biological Sciences)
MSA Distinguished Scientist (Physical Sciences)
MSA Burton Medal
MSA Outstanding Technologist (Biological Sciences)
MSA Outstanding Technologist (Physical Sciences)
MSA Morton D. Maser Distinguished Service Award


Distinguished Scientist Awards: These Awards honor preeminent scientists
from both the Biological and Physical disciplines who have an
internationally recognized record of outstanding achievements in the field
of microscopy and microanalysis.

Burton Medal: The Burton Medal was initiated to honor the distinguished
contributions to the field of microscopy and microanalysis of a scientist
who is not older than 35 years of age in the January of the award year.

Outstanding Technologist Awards: These Awards honor technologists from both
the Biological and Physical Sciences who have made significant contributions
such as the development of new techniques which have contributed to the
advancement of microscopy and microanalysis.

Morton D. Maser Distinguished Service Award: This Award was initiated to
recognize outstanding volunteer service to the Society as exemplified by
Mort Maser, who served the Society for many years with great dedication.
This award is made to honor an MSA member who has provided significant
volunteer service to the Society over a period of years.


The Distinguished Scientist, Burton Medal, and Outstanding Technologist
Awards Nominations should include:
1) a letter from the primary MSA nominator describing the research
accomplishments of the candidate with particular emphasis on the unique
technical achievements in the Physical or Biological Sciences; and
2) supplemental letters of support from other members of the scientific
community.

The Morton D. Maser Distinguished Service Award Nomination should include:
1) a letter from the primary MSA nominator describing the basis for the
nomination; and
2) supplemental letters of support from other members of MSA.

The Deadline for receipt of Awards Nomination Packages is December 31, 1997.
Please contact the MSA Awards Committee [Gracie Burke (mgburke-at-pitt.edu),
Stan Erlandsen (stan-at-lenti.med.umn.edu), Bev Maleef
(Beverly_E_Maleeff-at-sbphrd.com), Jim Bentley (bentleyj-at-ornl.gov), Bill
Gunning (wgunning-at-magnum.mco.edu) ] or the MSA Business Office for
additional information. NOTE: E-mail "letters" are acceptable!


Thanks!

Gracie Burke




Dr. M.G. Burke
Westinghouse Electric Corp.
Bettis Laboratory
West Mifflin, PA 15122
tel: (412) 476-5883; fax: (412) 476-5151
e-mail: mgburke-at-pitt.edu







From: Wolfgang Muss :      W.Muss-at-lkasbg.gv.at
Date: Mon, 29 Sep 1997 11:29:04 +-200
Subject: =?iso-8859-1?Q?LM=2C_EM=2C_BUFFER=2C_Millonig=B4s?=

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Dear Douglas, 09/29/97
original recipe for MILLONIG=B4s buffer was published as follows:
MILLONIG G: Advantages of a Phosphate Buffer for OsO4-Solutions in =
Fixation.
Abstract B 26, publ. on behalf of the EMSA in:
Journal of Applied Physics (LANCASTER =3D Procs of the ann. Meetings of =
EMSA) 32, p.1637 (1961)
Original Text of this abstract:

} } Since the sodium-mono- and diphosphate exists as an effective buffer =
system in the body fluids of animals, it has seemed reasonable to test =
it as a vehicle for OsO4 in fixation of biological tissues. An isotonic =
(delta=3D -0.56 degrees centigrade) solution at pH 7.3 of the following =
composition was tested:
sol. a) 2,26% NaH2PO4
sol. b) 2.52% NaOH
sol. c) 5.4% Glucose (about 5 ml added to an end volume of OsO4-soln. =
of 50 ml.)

[sol. d): 41,5 ml sol. a) + 8.5 ml sol. b)
[fixative: 45 ml sol. d) + 5 ml sol. c) + 0.5g OsO4 (as crystals)]
[It has been found that this solution is stable for several weeks, if =
stored in the refrigerator in a clean bottle. In comparative studies, =
mainly with the veronal-acetate buffer + Osmium and on different animal =
tissuies, the phosphate buffer seems to prevent extraction of the =
cytoplasmic matrix, preserves the glycogen and fibrillar elements and =
gives an uniform fixation at different levels in the tissue block { {]
--------End of original abstract text of Millonig himself.---------

Recipe for convenient lab-volumes therefore:
for an isotonic buffer solution ( ~ 300 mosmol, pH ~ 7.2 - 7.3 ) u =
should mix the following:
5.65 g NaH2PO4.H2O ad 250 ml of A.dest ( =3D 33.9 g ad 1500 ml)
1.26 g NaOH-pastilles ad 51 ml A.bidest ( =3D 7.76 g ad 307 ml)
makes: ~300 ml ( ~ 1800 ml)

You should measure pH, as well as osmolality:
in my experience osmolality is a little bit lower than 300 mosmols
therefore I have to add about 0.1 to 0.2 (w/v) of D-Glucose (~0.5 g, or =
~3.3g respectively, for endvolume of 1800 ml) to end up with 300 =
mosmols.

MILLONIG=B4s 0.13 M sodium phosphate buffer (isoosmotic with mammalian =
blood)
(from: MILLONIG G. (ed): Laboratory Manual of Biological Electron =
Microscopy; published and distributed by Mario SAVIOLO-Editore, =
VERCELLI/ITALIA, copyright by G.Millonig, 1976 , 67 pages)
Solution A: 0.164 M monosodium phosphate ( =3D 2.26% NaH2PO4.H2O,
=3D 2.56% NaH2PO4 .2H2O)
Solution B: 0.63 N sodium hydroxide (=3D 2.52% NaOH)

pH 6.0 6.2 6.4 6.8 7.0 7.2 =
7.4 7.6 7.8
ml A 96.2 94.7 92.5 87.9 85.8 83.9 82.5 =
81.6 80.8
ml B 3.8 5.3 7.5 12.1 14.2 16.1 =
17.5 18.4 19.2=20

or by dissolving in 100 ml H2O:

pH 6.4 6.8 7.0 7.2 =
7.4 7.6 7.8
NaH2PO4 . H2O g 1.3 0.9 0.7 0.51 0.34 0.23 0.16
+
Na2HPO4.7 H2O g 0.9 1.72 2.1 2.5 2.82 3.0 3.19
or
Na2HPO4.12H2O g 1.2 2.3 2.8 3.35 3.77 =
4.03 4.26

Hope this helps for the solution of your request.
Best regards

Wolfgang MUSS
EM-Lab LKA Salzburg AUSTRIA/Europe.
END of MESSAGE, No attachment added.




From: NICK SCHRYVERS :      nick_schryvers-at-ematserv.ruca.ua.ac.be
Date: 29 Sep 1997 14:16:03 +0100
Subject: for Dr. H.Y. Peng (Shenyang

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Memo : for Dr. H.Y. Peng (Shenyang) 29-09-1997
14:11

Dear Dr. Peng,

Please send me your e-mail and fax coordinates ?

Sincerely,


Dr. Dominique Schryvers
University of Antwerp, RUCA - EMAT
Groenenborgerlaan 171, B-2020 Antwerpen (Belgium)
Tel: 32-3-2180247 Fax: 32-3-2180257
e-mail: schryver-at-ruca.ua.ac.be
homepage: http://www.ruca.ua.ac.be/~EMAT/Schryvers.html




From: Duncan Waddell :      emdwadde-at-dingo.cc.uq.edu.au
Date: Mon, 29 Sep 1997 07:48:36 -0500
Subject: Specimen holders for sale

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X-Sender: zaluzec-at-microscopy.com
Message-Id: {v03007801b0555415ba32-at-[206.69.208.21]}
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The Centre for Microscopy and Microanalysis at the University of
Queensland has the following specimen holders surplus. All holders
to be sold in working order, the cold stage controller is not included.
Interested parties should contact,

Assoc. Prof. Alasdair W. McDowall
Deputy Director: Center for Microscopy & Microanalysis
University of Queensland, Brisbane. QLD 4072
Phone: (07) 3365-4211
International: 61 (7) 3365-4211
Facsimile: (07) 3365-4422
International: 61 (7) 3365-4422
WWW: http://www.uq.oz.au/nanoworld/nanohome.html


EXCESS SPECIMEN HOLDERS DUE TO JEOL 4000FX-4010 UPGRADE
JEOL 4000FX SPECIMEN HOLDERS
(a) GATAN (D8020106-1) Normal double tilt, motorised, low
background. Holder has had routine use.
(b) GATAN (D8020105) Tilt holder, motorised rotation. Holder has
not been used.
(c) JEOL standard single tilt holder. Holder has had minimum use.
Also
Hitachi 200kV TEM H 800 Specimen Holder
(d) GATAN ( D003130 ) LN2 holder. Holder has had minimum use.


Cost:
Aus$ 5,000.00 per holder plus shipping.


--
************************************************************
Duncan Waddell (BSc)
Centre for Microscopy and Microanalysis
The University of Queensland. St. Lucia. Qld. 4072
Telephone: +61-7-3365-4216
Facsimile: +61-7-3365-2199
WWW: http://www.uq.edu.au/nanoworld/nanohome.html
************************************************************
Any opinion expressed is that of the writer,
and not necessarily that of CMM or of the University.
************************************************************






From: Yves Maniette :      yves-at-giga.sct.ub.es (by way of Nestor J. Zaluzec)
Date: Mon, 29 Sep 1997 07:52:09 -0500
Subject: effect of objective aperture in charging

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All,

Following the thread about coating of a glass sample, Jacky Larnould added
off line that when the objective aperture is set (above the sample) the
"charging effect" disappears, and the sample does no break. While I have
noticed this phenomenon for a while I have never come with a satisfactory
explanation. Has anyone found a good explanation to this?

Yves Maniette






From: YIMIN YAO :      yimin-at-fy.chalmers.se
Date: Mon, 29 Sep 1997 07:51:27 -0500
Subject: etching agent for Cu-Co

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Hello,

I need a solution to etching a Cu-Co alloy ( {90:10 wt% ). Could anyone
give any suggestions for the suitable chemical or electrolytic agents.


Thanks in advance.


Regards,

Yimin


Drs. Yimin Yao

Miroscopy and Microanalysis Group
Chalmers University of Technology
Dep. of Physics
S-41296 G=F6teborg
Sweden

TEL 46 31 772 3633
=46AX 46 31 772 3224






From: feldsdm4-at-juniata.edu ()
Date: Mon, 29 Sep 1997 07:53:22 -0500
Subject: microwave staining technology

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Email: feldsdm4-at-juniata.edu
Name: David Feldser

School: Juniata College


Question: Where can i find a good source of information
about microwave staining technology for the
electron mircroscope?

---------------------------------------------------------------------------






From: Tony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Mon, 29 Sep 1997 08:58:35 -0400
Subject: Re: TEM of nonconductors-carbon coat both side?

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} I am finally starting to prepare some TEM samples of glass. Does anyone
} know whether a light coat of carbon should be applied to both sides of the
} sample or is just one side (I assume the top side in the microscope) good
} enough?
}

We only ever coat one side. We usually try to put the coated side towards
the electron source, but not always. It has always worked fine in
preventing charging. I've often wondered why this should be, but it
certainly works, on either ceramics or polymers!

Tony.



Anthony J. Garratt-Reed
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
United States of America

Ph: 617-253-4622
Fax: 617-258-6478





From: Linda Iadarola :      linda.iadarola-at-yale.edu
Date: 29 Sep 1997 08:58:03 -0400
Subject: Re: frozen thin sections of

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From: Bengt Stocklassa :      bengt-at-mail.xco.se
Date: Mon, 29 Sep 1997 15:44:25 +0200
Subject: Titanium-staining

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Reply to: RE} frozen thin sections of isolated cells

Dear Christine and David,
We routinely prepare culture cell pellets for ultra-thin cryosectioning but we
tend to put the resuspend the cells in gelatin then pellet this to form a nice
gelled block of cells which can be treated the same as tissue for
cryosectioning. Here is a copy of our protocol. If you have any questions
please feel free to call me at 203-785-3646. You may also want to try adjusting
the temperature and thickness you are cutting your cells. This may alleviate
the "snow" effect.
Linda Chicoine
Center for Cell Imaging
yale Univ. School of Medicine
linda.iadarola-at-yale.edu
EMBEDDING CELLS IN GELATIN/SUCROSE

1. Fix cells in buffered fixative directly in the culture dish. Can fix for
30' to overnight at 4C.

2. Remove fixative and replace with PBS/10%FCS to cover monolayer.

3. Scrape cells off culture dish with teflon , use glass pippette to transfer
cells to an eppendorf tube.

4. Centrifuge gently to form a loose pellet.

5. Remove half the PBS/FCS and replace with 10% gelatin at a 1:1 dilution with
PBS/FCS still in the tube.
Final concentration 5% gelatin. Resuspend cells in this and re-centrifuge
gently to form a pellet again.

6. Place tube in ice bucket for 15-30' or in fridge for longer, until gelatin
has hardened.

7. Cut bottom of tube with a razor blade. Cut straight through tube to get the
gelled pellet in the bottom
piece of the tube.

8. In a petri dish of buffer or sucrose sitting on ice, remove the cell pellet
from the bottom of the tube using a shaved wooden stick. Under a dissecting
microscope, cut the pellet into small triangular or rectangular pieces.

9. Place peices in eppendorf tube filled with sucrose. Leave pieces for 30' or
longer to infiltrate.

10. Pour sucrose into small petri dish, on ice,under disecting microscope.
Remove a piece using capillary action of a fine point forceps and place the
piece on a specimen nail. Check for excess sucrose around the pellet. Remove
excess sucrose with a wedge of filter paper.

11. Immediately plunge the nail with the specimen into liquid nitrogen. Swirl
the specimen somewhat
vigorously and when completely frozen place the specimen in a nunc tube on an
aluminum storage cane. Specimens can remain on the cane in liquid nitrogen for
several years.


--------------------------------------

We have a problem obtaining frozen ultra-thin sections of cultured cells.
We have tried both 2.3 M sucrose and 20% PVP/1.8 M sucrose to infiltrate
the cells before freezing without success. Briefly, after fixing the cells
and rinsing with buffer, the plates are scraped and the cells are
microfuged briefly to pellet the cells. Then we add a small amount
(approximately 1X the volume of the cell pellet) of sucrose or PVP/sucrose
to the pellet of cells, and tap the tube to resuspend the cells. The next
day a small amount of the cell suspension is placed on a metal peg and
frozen in liquid nitrogen. When sectioning we obtain snow-like flakes on
the knife edge. Tissues processed in a similar manner cut well. We
suspect the problem to be too much buffer associated with the cell pellet,
even though we try to remove as much buffer from the pellet as possible.
Any suggestions would be appreciated.

Christine Roy and David Hall
Albert Einstein College of Medicine
Bronx, NY

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Hi Folks !

Does anyone of you know of some specific staining technique for Titanium,
and where to find a cook-book for it? We need it for recovery of the new
type of Gun Shot Residues.

Best regards
Bengt
Bengt Stocklassa , Managing Director
Cox Analytical Systems AB | Phone: +46 31 7725300
House of Innovations, CTH | Fax: +46 31 7725600
412 96 - Gothenburg, SWEDEN | E-mail: bengt-at-xco.se





From: ejb11-at-psu.edu (Edward J. Basgall)
Date: Mon, 29 Sep 1997 10:20:48 -0500
Subject: Re: fungal hyphae - critical point dry/freeze dry

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Hi Jill,
I've done some preliminary work using Leica's FD unit on fungal material.
I've FD Bergamot (Monarda didyma) leaves with a powdery mildew fungal
infection and yeast (Sacc. cer). The leaves came out fine but the fungal
hyphae were collapsed. The yeast came out very nicely. I ran both fixed
(Karnovsky's) and unfixed pieces. All were cryo prepped by propane plunge
freezing. All were examined at 2.0 kV uncoated. Overall I was pleased
with the results, I haven't had a chance to go over all of the samples but
the chemical fixation seemed to be better for maintaining the fungus. I
would run both unfixed and fixed as I believe the mechanical agitation of
adding a liquid solution may have washed away some fungal material.
Aldehyde and/or Osmium vapor fixation would likely be less disruptive.

My FD time/temp schedule was:
48hrs-at--80C
36hrs-at--60C
24hrs-at--40C
12hrs-at--30C
12hrs-at--15C
24hrs-at--5C
6hrs-at-+10C
30hrs-at-+20C
Vacuum was maintained with a cryo sorption pump.

I can post some images to my web page by the end of the week, if you are
interested. The unfixed yeast pics are there already. Follow the links
from Research Projects to Sample prep for SEM...
http://www.personal.psu.edu/ejb11

cheers
ed

Edward J. Basgall, PhD
The Pennsylvania State University
Surface Chemistry Group ejb11-at-psu.edu
Materials Research Institute Building Ph: 814-865-0493
University Park, PA 16802-7003 FAX: 814-863-0618
http://www.personal.psu.edu/ejb11/






From: Warren Straszheim :      wes-at-ameslab.gov
Date: Mon, 29 Sep 1997 09:44:06 -0500
Subject: RE: users of link izis 300

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Let me modify my earlier suggestion for using the PrintScreen copying method
to concur with Rainer's suggestion below. The PrintScreen trick still works,
but other methods are probably more suitable when available. And this
package does allow for copying the spectrum to the Windows clipboard.

You should be advised that you will probably have to use the Paste Special
function to select the spectrum "picture" for pasting instead of the sample
id "text". The text will come up as default.

At 08:51 PM 9/26/97 +0200, Rainer wrote:
} } } Malgorzata Warmuzek wrote:
} } } I have a problem with an import the file with an extension *.sp ( spectrum )
} and put it to the } } document in word 6 or amipro
}
} Dear Malgorzata,
}
} I use the ISIS suite 3.0 and there is an easy way to copy spectra to any other
} windows program, maybe it works also with your version. Use the command
} 'Buttons' 'Print' and a new window will appear called 'Spectrum printing'.
} There it is possible to activate the command 'Edit' 'Copy'. The Spectrum will
} now be transfered to the clipboard in form of a vector graphic.
}
} Kind regards
}
} Rainer
----------------------------------------------------
Warren E. Straszheim
23 Town Engineering, or
270 Metals Development
Iowa State University
Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.marl.iastate.edu/marl/ (re: SEM)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)

electron microscopy, x-ray analysis, image analysis
computer applications
coal characterization and processing





From: blazquez-at-rockefeller.univ-lyon1.fr (Francisco Javier Hernandez Blazquez)
Date: Mon, 29 Sep 1997 16:47:37 +0200 (MET DST)
Subject: parafin thick sections

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Dear Colleagues
I'm trying to cut 60 um parafin sections, but I've found it very difficult.
It's rare to botain a good section
There is some procedure to facilitate this work

Francisco Javier Hernandez Blazquez
Centre commun de Quantimetrie, 8 avenue Rockefeller
69373 LYON CEDEX 08. France. Tel : (33) 4 78 77 75 19.






From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Mon, 29 Sep 1997 10:11:39 -0500
Subject: Re: effect of objective aperture in charging

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Responding to the message of {v03007804b05554e7eb94-at-[206.69.208.21]}
from Yves Maniette {yves-at-giga.sct.ub.es} (by way of Nestor J. Zaluzec):
} Following the thread about coating of a glass sample, Jacky Larnould added
} off line that when the objective aperture is set (above the sample) the
} "charging effect" disappears, and the sample does no break. While I have
} noticed this phenomenon for a while I have never come with a satisfactory
} explanation. Has anyone found a good explanation to this?
}
} Yves Maniette
}
We looked at this some time ago. JEOL machines work better than Philips, unless
you install a really large aperture (we use 800 micron) and move to that
aperture rather than moving the apertures out of the beam. The lack of symmetry
induced by moving the aperture rod to the side rather than moving to an empty
hole prevents the system from effectively stabilizing the charge, and the beam
is displaced from the specimen.

The beneficial effect of the aperture is probably due to some capacitative
charge dissipation from the sample by the very close proximity of the aperture.
It is important to have everything properly aligned - beam and aperture, or the
lack of radial symmetry will again deflect the beam away from the area of
interest.

Different specimens respond differently - we have had very good success with
single crystal alumina, but less success with polycrystalline specimens.

Good luck.

Stuart


__________________
Stuart McKernan stuartm-at-tc.umn.edu
Microscopy Specialist
CIE Characterization Facility, University of Minnesota Phone: (612) 626-7594
100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590





From: William D Meek :      meekwd-at-okway.okstate.edu
Date: Mon, 29 Sep 97 10:44:56 -0600
Subject: TEM: Gap Junctions

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We are trying to calculate the surface area of gap junctions. Has
anyone done something similar to this? Are the gap junctional
complexes (groups of connexons) arranged in a circular profile?
If you could provide me with a reference or any information, I would
appreciate it.

Bill Meek
Meekwd-at-okway.okstate.edu






From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 29 Sep 97 12:11:09 -0500
Subject: Tire particules ID

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

With regard to the following:
=================================================
We are looking for any information we can find on the
} microscopy/identification of particles from automobile tires. Seems we
} have a case where we need to try to match particles from a pair of tennis
shoes to a particular set of automobile tires(best case), or at least
identify particles as possibly coming from automobile tires (more likely).
=================================================
Assuming what you are saying is you have a need to demonstrate "common
origin", which is not all that of an uncommon request, we have found the
best approach is to use thin section TEM on the particulates. Tire-origin
particulates have a nice characteristic dispersion of carbon black plus
other inorganics.

The elastomeric system found in tennis shoes also has inorganic additions
present. Generally speaking (based on our own in-house experience) the
additive particles are larger than would be the carbon black and other
particles found in a tire. Hence to differentiate between tires vs. tennis
shoes, this should not be a major problem. If you wanted to show that the
particulates came from a specific tire or a specific pair of tennis shoes,
this would be a much higher level question, and considerable additional work
(and the running of far more samples) would be indicaed.

The reason why this is more of a TEM than SEM request is that the
particulates, especially the carbon black, is really below the practical
limit of SEMs in terms of resolution and furthermore, the TEM view of this
kind of sample is far easier to understand and interpret. And when EDS does
have to be done, that data also is far easier to interpret and understand
(because you don't have to deal with "depth" effects).

All thin sectioning on these kinds of samples must be done with the use of a
good ultramicrotome, using a cryo stage, and using diamond knives, and we
would always use a "Materials Science" diamond knife in order to not
unnecessarily balloon up the cost of doing this kind of work.

Disclaimer: Our Structure Probe laboratories offer this kind of laboratory
analytical service for clients needing to have done this kind of work. We
are also a major provider of "materials science" diamond knives for persons
wanting to do this kind thin sectioning.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From: SEMTRADER-at-aol.com
Date: Mon, 29 Sep 1997 15:14:17 -0400 (EDT)
Subject: Used Equipment for Sale (SEM/TEM X-ray)

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Please visit our website.

http://home.aol.com/SEMTRADER

Thank you





From: Ziel Rainer :      Rainer.R.Ziel-at-Obernburg.ARLO.akzo.nl (Tel 49\(0\)6022-812645)
Date: Mon, 29 Sep 1997 22:22:22 +0200
Subject: RE: users of link izis 300

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Charlie Kong wrote:
} } Rainer,
} } Have you found that the spectrum you got it by copying become a low
resolution (287x287 pixels) graph?
} } I have tried the method you described in the internet, and felt the
difficulty to see the lebels of axis...
} } p.s. I pasted the spectrum in Paint Shop Pro.
} } Charlie Kong kong-at-t-rex.materials.unsw.edu.au

Dear Charlie,

The spectrum is copied to the clipboard in form of a vector graphic.
PaintShopPro is a pixel graphic program.
So the spectrum is converted when it is imported. You can import the graphic
directly to your Word processor.
In the german version of Winword it is done by the command 'Einfugen' 'Inhalte
Einfugen' 'Graphic'. Afterwards
it is possible to double click the graphic and to change e.g. labels.

Kind regards

Rainer

-------------------------------------------
Rainer Ziel
Akzo Nobel Central Reasearch
63784 Obernburg - Germany




From: Kalman Rubinson :      rubinsnk-at-is2.nyu.edu
Date: Mon, 29 Sep 1997 15:54:35 -0400 (EDT)
Subject: Re: parafin thick sections

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On Mon, 29 Sep 1997, Francisco Javier Hernandez Blazquez wrote:

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}
} Dear Colleagues
} I'm trying to cut 60 um parafin sections, but I've found it very difficult.
} It's rare to botain a good section
} There is some procedure to facilitate this work

A neat old trick for doing this is to use rubber cement in the mixture.
It gives the paraffin the needed tensile strength to prevent crumbling.

Kalman Rubinson





From: Gary R. Login :      glogin-at-bidmc.harvard.edu
Date: Mon, 29 Sep 1997 17:46:46 -0400
Subject: Re: microwave staining technology

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David Feldser asked for references on microwave-accelerated staining
for electron microscopy. I list 14 references FYI:


1. Estrada JC, Brinn NT, Bossen EH: A rapid method of staining
ultrathin sections for surgical pathology TEM with the use of the
microwave oven {italic} . {/italic} Am J Clin Pathol 1985, 83:639-641


2. Zondervan PE, de Jong A, Sorber CWJ, Kok LP, de Bruijn WC, van der
Kwast TH: Microwave stimulated incubation in immunoelectron microscopy:
a qualitative study {italic} . {/italic} Histochem J 1988, 20:359-364


3. Matsutani S, Yamamoto N: Improved methods for immuno-electron
microscopy of cultured cells: use of a novel substrate and application
of microwave irradiation {italic} . {/italic} Acta Histochem Cytochem
1990, 23:227-236


4. Wouterlood FG, Boon ME, Kok LP: Immunocytochemistry on free-floating
sections of rat brain using microwave irradiation during the incubation
in the primary antiserum: light and electron
microscopy {italic} . {/italic} J Neurosci Methods 1990, 35:133-145


5. Mizuhira V, Hasegawa H, Notoya M: Microwave irradiation staining
method for biological electron microscopy {italic} . {/italic} Histochem J
1992, 24:596(abstract)


6. van Deuren B, van Reempts J, Borgers M: Microwave-enhanced silver
staining of degenerating neuronal processes {italic} . {/italic} Eur J
Morphol 1992, 24:597(abstract)


7. Giammara BL, Hopfer RL, Yates PE, Hanker JS: A rapid silver stain
for the DNA of microorganisms cultured from AIDS or other
immunocompromised patients {italic} . {/italic} Proc Twelfth Int'l Congr
Electron Micros 1990, 48:762-763


8. Hanker J, Giammara B: Microwave-accelerated cytochemical stains for
the image analysis and the electron microscopic examination of light
microscopy diagnostic slides {italic} . {/italic} Scanning 1993, 15:67-80



9. Leong AS-Y: Microwave techniques for diagnostic
laboratories {italic} . {/italic} Scanning 1993, 15:88-98


10. Ainley CD, Ironside JW: Microwave technology in diagnostic
neuropathology {italic} . {/italic} J Neurosci Methods 1994, 55:183-190


11. Utsunomiya H, Shan L, Kawano I, Iwasaki A, Ono K, Kobayashi A, Kuma
K, Kishikawa S, Kakudo K: Immunolocalization of parathyroid hormone in
human parathyroid glands with special references to microwave antigen
retrieval {italic} . {/italic} Endocr Pathol 1995, 6:223-227


12. Stirling JW, Graff PS: Antigen unmasking for immunoelectron
microscopy: labeling is improved by treating with sodium ethoxide or
sodium metaperiodate, then heating on retrieval
medium {italic} . {/italic} J Histochem Cytochem 1995, 43:115-123.


13. Login GR, Dvorak AM. Microwave fixation and microwave staining
methods for microscopy. In: Hayat MA ed. Immunogold-silver staining:
methods and applications. Boca Raton, CRC Press, 1995, pp. 163-182


14. Login GR, Dvorak AM: The Microwave Toolbook. A Practical Guide for
Microscopists. Boston, Beth Israel Hospital, 1994, pp. 184



Please contact me if you have any questions.





Gary R. Login, D.M.D., D.M.Sc.

Dept. Pathology

Beth Israel Deaconess Medical Center

330 Brookline Avenue

Boston, MA 02215


phone: 617-667-2034

fax: 617-667-8676


e-mail: glogin-at-bidmc.harvard.edu





From: Barbara Foster :      mme-at-map.com
Date: Mon, 29 Sep 1997 18:10:37 -0700
Subject: LM course @ Providence College

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Reminder: "Optimizing Light Microscopy" will be hosted by the Biology
Department of Providence College this Friday, October 3. Course cost:
$150,includes a copy of "Optimizing Light Microscopy for Biological and
Clinical Laboratories". Despite the title of the book, the course has
wide application to light microscopy in any venue.

For further information, write, call, or email:

Barbara Foster
President
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email:mme-at-map.com
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From: Barbara Foster :      mme-at-map.com
Date: Mon, 29 Sep 1997 18:10:37 -0700
Subject: LM course @ Providence College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Reminder: "Optimizing Light Microscopy" will be hosted by the Biology
Department of Providence College this Friday, October 3. Course cost:
$150,includes a copy of "Optimizing Light Microscopy for Biological and
Clinical Laboratories". Despite the title of the book, the course has
wide application to light microscopy in any venue.

For further information, write, call, or email:

Barbara Foster
President
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email:mme-at-map.com
---------------------------------------------------------------------------------------------------------------------------------
********** Microscopy/Microscopy Education **********
America’s First National Consortium of Microscopy Experts
Specializing in Customized, On-site Training
in all areas of Microscopy, Sample Prep, and Image Analysis




From: Barbara Foster :      mme-at-map.com
Date: Mon, 29 Sep 1997 18:12:46 -0700
Subject: Fluorescence microscopy course @ Providence College

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Reminder: "Inside Fluorescence", a lecture-demonstration on factors
affecting fluorescence microscopy, will be hosted this SATURDAY, OCTOBER
4, by Providence College.

Cost of class: $150 which includes a detailed course workbook.

For further information, please contact our office.

Barbara Foster
President
Microscopy/Microscopy Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email:mme-at-map.com
---------------------------------------------------------------------------------------------------------------------------------
********** Microscopy/Microscopy Education **********
America’s First National Consortium of Microscopy Experts
Specializing in Customized, On-site Training
in all areas of Microscopy, Sample Prep, and Image Analysis




From: Melvyn Dickson :      M.Dickson-at-unsw.edu.au
Date: Tue, 30 Sep 1997 08:21:53 +1000
Subject: Re: effect of objective aperture in charging

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Hello World,

The best explanation for the effect of the objective aperture was published
(I recall) in a Philips bulletin.

The section in a TEM has a positive charge generated in it as the primary
beam produces secondary electrons in the section which then exit from it.
This charge will destroy the section if it is not neutralised or conducted
away through e.g. a carbon coat.

The objective aperture neutralises the positive charge in the specimen by
reflecting back backscattered and secondary electrons which re-enter the
section.


Mel Dickson
Electron Microscope Unit,
University of New South Wales.
Sydney NSW 2052 Australia

Phone (+612) 9385-6383
Fax (+612) 9385-6400

Website {http://emunit1.babs.unsw.edu.au/emu_top.htm}




From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Mon, 29 Sep 1997 15:08:46 -0700
Subject: Re: effect of objective aperture in charging

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Dear Yves,
In every TEM I've seen, the objective aperture slides in just below the
sample. In
samples that are likely to break, such as formvar-covered slots, I was told
never to
look at the sample without the objective aperture in. Having all the high-kV
electrons
hitting on just the top side would pop the film. If the objective aperture
is in, the
high-kV electrons scattered back from the aperture below the specimen will
balance
the flux from above and the film probably won't break. BTW, it doesn' matter
which
side of the sample you coat, since the electrons go right through, anyway.
Otherwise,
it wouldn't be TEM.
You wrote:
} All,
}
} Following the thread about coating of a glass sample, Jacky Larnould added
} off line that when the objective aperture is set (above the sample) the
} "charging effect" disappears, and the sample does no break. While I have
} noticed this phenomenon for a while I have never come with a satisfactory
} explanation. Has anyone found a good explanation to this?
}
} Yves Maniette
}
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: Harry Wachob :      sfhfw-at-fail.com
Date: 29 Sep 1997 16:35:39 -0700
Subject: Commercial ESEM

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I am interested in purchasing some time on a commercial ESEM to do some
microscopy of fractures. Does anyone know of any ESEMs in CA, AZ, OR or WA
that will provide commercial time? If so please email me at HWachob-at-fail.com.
Thank You for your assistance.





From: P.M. HOUPT :      houpt-at-worldaccess.nl (by way of Nestor J. Zaluzec)
Date: Mon, 29 Sep 1997 20:57:02 -0500
Subject: correctionlens design

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X-Sender: zaluzec-at-microscopy.com
Message-Id: {v03007803b0560ce52665-at-[206.69.208.21]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

dear microscopists,

Problem: I do have a excellent Zeiss stand and a complete series of
Leitz planapochromatic objectives.
However as you probable know Zeiss microscopes have a 160 mm mechanical
tubelength whereas teh Leitz objectives are calculated for 170 mm.
What kind of correction lens can I use in between the tube so that I can
use the Leitz lenses on the Zeiss stand without loose of optical
quality?
Can anybody give me a good advice .

thank you in advance,

Pieter Houpt

(the Hague ,the Netherlands)






From: Cordula Rodemann :      cordula.rodemann-at-uni-tuebingen.de
Date: Tue, 30 Sep 1997 09:41:16 +0200
Subject: Re: microwave staining technology

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---------- Forwarded message ----------

feldsdm4-at-juniata.edu wrote:
}
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}
} Email: feldsdm4-at-juniata.edu
} Name: David Feldser
}
} School: Juniata College
}
} Question: Where can i find a good source of information
} about microwave staining technology for the
} electron mircroscope?
}
} ---------------------------------------------------------------------------


Hi David,

this Link might be helpful http://www.rfglobalnet.com

Cordula Rodemann




From: Krzysztof Jan Huebner :      hubner-at-czapla.IOd.krakow.pl
Date: Tue, 30 Sep 1997 10:19:50 +0200 (MET DST)
Subject: Zaroszenie na seminarium

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Szanowni Panstwo,
milo jest mi zawiadomic ze;


Instytut Odlewnictwa
Zespol Laboratoriow Badawczych
Krakow, ul Zakopianska 73

Ma zaszczyt zawiadomic, ze w dniu 10 pazdziernika
o godz. 11 w Instytucie Odlewnictwa w Krakowie:

Dr Jean Luis CHERMANT
z ISMRA - LERMAT - Caen Francja, wyglosi referat pt:

Pelzanie kompozytow ceramicznych



Dr Carl REDON
z ISMRA - LERMAT - Caen Francja, wyglosi referat pt:

Morfologia makroporowatoci w zbrojonych wloknami betonami,
jej wplyw na wytrzymalosc

Serdecznie zapraszamy wszystkich zainteresowanych.
Referaty w calosci beda tlumaczone na jezyk polski.

Do spotkania na seminarium


Krzysztof Jan Huebner

{hubner-at-IOd.krakow.pl} :-)

FOUNDRY RESEARCH INSTITUTE
Research Materials Department
Structural and Physical Research Laboratory

Zakopianska 73 Call +48 12 2605022 ext. 356
30-418 KRAKOW - POLAND Fax +48 12 2665478, +48 12 2660870





From: Karlene Hewan-Lowe :      khewanl-at-emory.edu
Date: Tue, 30 Sep 1997 07:34:21 -0400 (EDT)
Subject: Re: Controls for immunofluorescense

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--- ImmunoFluorescence Controls for Renal Biopsies------
From T. Fores:
} Anyone doing immunofluorescenst techniques in renal biopsies running a control?
}
} From: Wanda
} Hi Teresa:
}
} At the present we are not running a control on our immunofluorescent
} techniques in renal biopsies. Has anyone replying back doing this?
Even if we pooled resources, there would not be enough material to
distribute to all laboratories that run this test. One solution is to run a
known positive patient sample at the time titre the antibody. Another
suggested solution was to run a tonsil control with each batch of patient
samples (simplifying solution - the test material in each tissue is
equivalent). And what about the control for the complement (C1, C3, C4) and
fibrinogen?

Business idea: rats with immune mediated GN, frozen kidney samples on slides ...

The situation regarding immunofluorescence controls (IF) for renal biopsies
is an example of the tension that exists between pure science and the
practical application of science in clinical laboratories. Manuals are
written and regulations are mandated without regard to the practical aspect
of running a test in a clinical laboratory. Some rational solution is needed
to stop laboratorians from resorting to draconian measures in order to
comply with the regulatory agencies.

} Also would you know of anyone who might be interested in serving as a
} consultant for EM or an EM Tech interested in relocating?
I can consult on Transmission EM of human tissues.
|--------------------------------|
| Karlene Hewan-Lowe, M.B., B.S. |
| Department of Pathology |
| Emory University |
| Phone: 404-686-2926 |
| Fax: 404-6864978 |
|--------------------------------|





From: Seth J. Grotelueschen :      sethg-at-CompuServe.COM
Date: Tue, 30 Sep 1997 08:16:17 -0400
Subject: Re: Image databases/archiving

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Message text written by INTERNET:MWIS-at-crf.cuis.edu
} microscopy-at-Sparc5.Microscopy.Com {

Check out PAX-it, an excellent image capture, archiving and databasing
system. It has pretty powerful report generation software which allows y=
ou
to effectively replace traditioinal film in the photo documentation
process. Going digital seems to pay for itself quickly. The other good
thing is that it has network software, so you can access the database of
images from your desktop PC's.

They are at www.mis-hq.com.




From: Materials Science International Services, GmbH :      info-at-msiwp.com
Date: Tue, 30 Sep 1997 13:04:14 +0100
Subject: Red Book available now

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------------------------------------------------------------------------
If you work in MATERIALS R&D, SOLID STATE PHYSICS, CRYSTALLOGRAPHY or in a
SCIENTIFIC LIBRARY, this is interesting for you.

A true compilation of all world literature data on MATERIALS CONSTITUTION;
PHASE DIAGRAMS and RELATED DATA is available in print:
the annual "RED BOOK" series .
It started with the publication year 1990 and is produced by MSI and the
Russian information service VINITI.

This compilation extracts data, diagrams and text from the world literature
and arranges the information after alloy systems (not after publications),
every year.
- No need to browse hundreds of journals + proccedings
- All foreign language publications translated into English
- Best coverage of "eastern" publications available anywhere.
- Uniformly structured detailed summaries of binary, ternary,...,
multicomponent systems.

The introduction package, comprises the publication years 1990 to 1995
inclusive. It provides over 5300 summaries, more than 6000 diagrams and
more than 1200 tables printed on over 8500 pages.
Have a look at sample summaries and order information at

http://www.msiwp.com or inquire at info-at-msiwp.com

NOTE: The very attractive introduction offer expires mid December `97.

-------------------------------------------------------------------------
This mailing list will inform you on the global phase diagram evaluation
program of MSI and its team MSIT. It will announce how you can access its
further products, such as the literature data base, electronic phase
diagrams, etc.
The traffic will be as low as 1 or 2 mailings per quarter.
If this information is of no interest to you, please accept our appologies
for the present message and send an e-mail to
majordomo-at-msiwp.com
with the message in the body: unsubscribe msi-adverts
This will remove your address from the list.











From: Martin Bartels :      bartels-at-uni-oldenburg.de
Date: Wed, 1 Oct 1997 03:02:48 +0100
Subject: TEM - Need help for embedding in LR white resin (plant roots)

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Dear Microscopists
I'm just beginning to use LR white as embedding medium for TEM- immuno-
cytochemistry. For previous embeddings of plant root segments for
conventional
TEM research with SPURRs resin, I used flat embedding mold of silicon
rubber for
simple orientation of the segments. Is it possible to use flat embedding
mold
also with LR white and how is it possible to avoid contact with oxygen
during
polymerization? What kind of molds are otherwise most suitable for LR
white?
Furthermore I'm looking for a common embedding procedure of plant roots in
LR
white for use in TEM immunogold-labelling. I would like to contact in this
way
a lab with similar interests.
Thanks to all comments.

Martin Bartels
FB Biologie / AG Pflanzenoekologie
C.v.O. University Oldenburg /Germany
PO Box 2503
26111 Oldenburg
phone ++49 441 798 3436 fax ++49 441 798 3436
e mail: bartels-at-uni-oldenburg.de





From: Yves Maniette :      yves-at-giga.sct.ub.es (by way of Nestor J. Zaluzec)
Date: Tue, 30 Sep 1997 08:05:47 -0500
Subject: EOT:effect of objective aperture...

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Here is a copy of replies I got about teh charging effect. One was sent
off line. I made an horrible mistake saying that the aperture is above the
sample, which is nonsense. Well let's say that I had a cold yesterday. YM.

All,

Following the thread about coating of a glass sample, Jacky Larnould added
off line that when the objective aperture is set (below the sample) the
"charging effect" disappears, and the sample does not break. While I have
noticed this phenomenon for a while I have never come with a satisfactory
explanation. Has anyone found a good explanation to this?

Yves Maniette

**********************************************************

capacitance effect between the sample and aperture - the smaller the
aperture the better for diffraction burgers vector analysis. Move the
aperture around and note what happens. Also compare Philips CM and Jeol
aperture geometries - you should find Philips easier to use with non
conducting samples owing to aperture being nearer to specimen. It's been a
while since I was doing this, however, when setting up diffraction
conditions, it's best to use large objective aperture when locating
yourself in the pattern. Removing the aperture entirely, as normally done,
causes massive beam tilt in strongly charged samples (e.g. dirty MgO).
cheers

Dr. Simon L. King

**********************************************************

We looked at this some time ago. JEOL machines work better than Philips,
unless
you install a really large aperture (we use 800 micron) and move to that
aperture rather than moving the apertures out of the beam. The lack of
symmetry
induced by moving the aperture rod to the side rather than moving to an empty
hole prevents the system from effectively stabilizing the charge, and the beam
is displaced from the specimen.

The beneficial effect of the aperture is probably due to some capacitative
charge dissipation from the sample by the very close proximity of the
aperture.
It is important to have everything properly aligned - beam and aperture, or
the
lack of radial symmetry will again deflect the beam away from the area of
interest.

Different specimens respond differently - we have had very good success with
single crystal alumina, but less success with polycrystalline specimens.

Stuart McKernan stuartm-at-tc.umn.edu

**********************************************************

In every TEM I've seen, the objective aperture slides in just below the
sample. In samples that are likely to break, such as formvar-covered
slots, I was told never to look at the sample without the objective
aperture in. Having all the high-kV electrons hitting on just the top side
would pop the film. If the objective aperture is in, the high-kV electrons
scattered back from the aperture below the specimen will balance the flux
from above and the film probably won't break. BTW, it doesn' matter which
side of the sample you coat, since the electrons go right through, anyway.
Otherwise, it wouldn't be TEM.

Mary Mager

**********************************************************

Hello World,

The best explanation for the effect of the objective aperture was published
(I recall) in a Philips bulletin.

The section in a TEM has a positive charge generated in it as the primary
beam produces secondary electrons in the section which then exit from it.
This charge will destroy the section if it is not neutralised or conducted
away through e.g. a carbon coat.

The objective aperture neutralises the positive charge in the specimen by
reflecting back backscattered and secondary electrons which re-enter the
section.

Mel Dickson
**********************************************************






From: Barbara Foster :      mme-at-map.com
Date: Tue, 30 Sep 1997 09:16:03 -0700
Subject: ASCB Mkt Research

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To: Manufacturers of microscopes, imaging and image analysis systems, and
sample preparation equipment

ASCB has informed us that there is still space available for MME to
conduct market research at the upcoming Cell Biology meeting. If there is
enough interest from you, we would be pleased to conduct a multi-client
survey, by subscription. This meeting provides a prime venue for testing
new ideas for instrument development. Since MME conducted research at
this meeting in 1993, there are also select opportunities for long-term
trend evaluation.

October 9th will be the deadline for our decision to go. Please note
that there will be only one block of questions available per technology
area and a total of approximately 25 questions, so space will be limited
to first come/first served.

For further information, please respond directly to:

Barbara Foster
Consortium President
Microscopy/Marketing & Education
53 Eton Street
Springfield, MA 01108
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com

Thanks!




From: James Martin :      James.S.Martin-at-williams.edu
Date: Tue, 30 Sep 1997 09:17:01 -0400 (EDT)
Subject: WWW sites for histology photos, blood

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Can anyone steer me to a website that has photomicrographs of histological
samples; in particular, blood samples?

Thanks.

James Martin
Williamstown Art Conservation Center







From: Seth J. Grotelueschen :      sethg-at-CompuServe.COM
Date: Tue, 30 Sep 1997 09:19:24 -0400
Subject: CCD Camera for Metallography

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Message text written by Randy Schnack
} Microscopy-at-Sparc5.Microscopy.Com {

The Leco system is the one that most are using. It uses a high res CCD
camera to provide film replacement using digital means. The software the=
y
provide is PAX-it, which does the archiving in an easy cabinet & folder
style database. It has a really cool "report generation" tool that links=

all of the data fields with the images in MS Word and gives you a great
print-out. =


The budget of 15k sounds about right for a complete computer, capture car=
d,
software AND high res camera. If the user wants to provide their own
pentium, that will save some money as well.

PAX-it is at www.mis-hq.com on the web.




From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 30 Sep 1997 09:33:09 -0500 (EDT)
Subject: Re: TEM of nonconductors-carbon coat both side?

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Dear Yves,
}
} One side carbon coating is sufficient. It can be on the top or the down
} side in the microscope, though you should get a better image if you place
} it on top,

The image should be the same regardless of which side is coated.
Assuming a perfect plane-wave for the incident beam, coating the bottom
will cause the image--assumed to be perfect--to be convoluted with the
scattering from the carbon; whereas, coating the top will form the image
with a beam which has been convoluted with the same scattering. Both
resulting images will be the same.
Yours,
Bill Tivol




From: Wolfgang Muss :      W.Muss-at-lkasbg.gv.at
Date: Tue, 30 Sep 1997 16:12:17 +-200
Subject: TEM, Embedding, EPON 815

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Greetings to all,
is there any person or EM-Lab out there who/which uses/used a resin =
formulation with EPON 815 (Polysciences).=20
Would be glad to receive a mixing recipe which works/worked as I have a =
small amount left for testing a staining procedure on semithin sections =
made from original Epon embedded blocks. If also a reference could be =
added it would be fine.
Thanking you in advance
Wolfgang MUSS, EM-Lab, Dept. Pathology, A-5020 SALZBURG/AUSTRIA, Europe
e-mail: W.Muss-at-lkasbg.gv.at




From: Mark E. Darus (216) 266-2895 General Electric Co. :      darus-at-cle.dnet.ge.com
Date: Tue, 30 Sep 97 10:26:53 EDT
Subject: Mailing an image from Voyager

Contents Retrieved from Microscopy Listserver Archives
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This is a question for the Noran Voyager 4 users. After I obtain
an image of my sample, on the monitor, is it possible to e-mail it from
the Voyager to a person on MS Exchange? Does it have to be converted into
a particular type of file? Does the person on the other end, the one
receiving the image, have to convert it and then view as a certain type of
file? Are instructions to do this in the Voyager manuals?

Thanks,

Mark Darus

Darus-at-cle.dnet.ge.com




From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 30 Sep 1997 08:04:25 -0700 (PDT)
Subject: Re: correctionlens design

Contents Retrieved from Microscopy Listserver Archives
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Hi would it be simpler to put a correction collar on the scope that would
extend your tube length to 170mm?

Bob

On Mon, 29 Sep 1997, P.M. HOUPT wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} dear microscopists,
}
} Problem: I do have a excellent Zeiss stand and a complete series of
} Leitz planapochromatic objectives.
} However as you probable know Zeiss microscopes have a 160 mm mechanical
} tubelength whereas teh Leitz objectives are calculated for 170 mm.
} What kind of correction lens can I use in between the tube so that I can
} use the Leitz lenses on the Zeiss stand without loose of optical
} quality?
} Can anybody give me a good advice .
}
} thank you in advance,
}
} Pieter Houpt
}
} (the Hague ,the Netherlands)
}
}
}





From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 30 Sep 1997 08:16:48 -0700 (PDT)
Subject: Re: TEM - Need help for embedding in LR white resin (plant roots)

Contents Retrieved from Microscopy Listserver Archives
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HI,

I flat embed LR White all the time. I got an old Vacume oven out of
surplus and hooked up a vacume pump and a dry nitrogen tank. When it is
time for polymerization and the temperature is stable at 55C, I purge the
chamber 3 times with nitrogen by pumping it down and letting in the
nitrogen. On the last perge, I fill the chamber with the nitrogen leaving
a slight vacume 1-3 lbs. Just to keep the door sealed. Polymerize for
24-48 hrs.

I use the peel-away polypropylene embedding molds. Dont use polystyrine.

On Wed, 1 Oct 1997, Martin Bartels wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Dear Microscopists
} I'm just beginning to use LR white as embedding medium for TEM- immuno-
} cytochemistry. For previous embeddings of plant root segments for
} conventional
} TEM research with SPURRs resin, I used flat embedding mold of silicon
} rubber for
} simple orientation of the segments. Is it possible to use flat embedding
} mold
} also with LR white and how is it possible to avoid contact with oxygen
} during
} polymerization? What kind of molds are otherwise most suitable for LR
} white?
} Furthermore I'm looking for a common embedding procedure of plant roots in
} LR
} white for use in TEM immunogold-labelling. I would like to contact in this
} way
} a lab with similar interests.
} Thanks to all comments.
}
} Martin Bartels
} FB Biologie / AG Pflanzenoekologie
} C.v.O. University Oldenburg /Germany
} PO Box 2503
} 26111 Oldenburg
} phone ++49 441 798 3436 fax ++49 441 798 3436
} e mail: bartels-at-uni-oldenburg.de
}
}





From: Lesley Weston :      lesley-at-unixg.ubc.ca
Date: Tue, 30 Sep 1997 10:01:01 -0700 (PDT)
Subject: Re: Glow discharge

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There is a paper from 1987 describing how to make a simple glow-discharge
unit. We made one from this design and have been using it successfully for
years, slightly modified - the needle-type valve doesn't seem to be
necessary, just close off the feed for the argon with a removable clamp.
The reference is : Aebi U. and Pollard T.D., A glow discharge unit to
render electron microscope grids and other surfaces hydrophilic. J.
Electron Microsc.Technique, 7:29-33 (1987). Hope this helps.
Lesley Weston.


On Thu, 25 Sep 1997, Gunnel Karlsson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Hello,
}
} I don't have access to a nice workshop, where they can construct a glow
} discharger, where in Europe can I buy one? Or, is it out there someone, who
} has an old mashine you don't need anymore?
}
} TIA
}
} Gunnel Karlsson
}
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} Gunnel Karlsson E-mail Gunnel.Karlsson-at-oorg2.lth.se
} Biomicroscopy Unit Tel +46 222 8229
} Inorganic Chemistry 2 Fax +46 222 4012
} Box 124
} S-221 00 LUND, Sweden
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} This message was sent by Eudora with recycled electrons
}
}
}





From: Lesley Weston :      lesley-at-unixg.ubc.ca
Date: Tue, 30 Sep 1997 10:01:01 -0700 (PDT)
Subject: Re: Glow discharge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is a paper from 1987 describing how to make a simple glow-discharge
unit. We made one from this design and have been using it successfully for
years, slightly modified - the needle-type valve doesn't seem to be
necessary, just close off the feed for the argon with a removable clamp.
The reference is : Aebi U. and Pollard T.D., A glow discharge unit to
render electron microscope grids and other surfaces hydrophilic. J.
Electron Microsc.Technique, 7:29-33 (1987). Hope this helps.
Lesley Weston.


On Thu, 25 Sep 1997, Gunnel Karlsson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Hello,
}
} I don't have access to a nice workshop, where they can construct a glow
} discharger, where in Europe can I buy one? Or, is it out there someone, who
} has an old mashine you don't need anymore?
}
} TIA
}
} Gunnel Karlsson
}
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} Gunnel Karlsson E-mail Gunnel.Karlsson-at-oorg2.lth.se
} Biomicroscopy Unit Tel +46 222 8229
} Inorganic Chemistry 2 Fax +46 222 4012
} Box 124
} S-221 00 LUND, Sweden
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} This message was sent by Eudora with recycled electrons
}
}
}





From: ebs-at-ebsciences.com
Date: Tue, 30 Sep 1997 13:56:30 EST
Subject: WWW sites for histology photos

Contents Retrieved from Microscopy Listserver Archives
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Dear fellow microscopists,

At 09:17 AM 9/30/97 -0400, James Martin wrote:
} Can anyone steer me to a website that has photomicrographs of histological
} samples; in particular, blood samples?

There are a number of such sites. There are annotated links to the best of
them from "The Histotech's Home Page" (http://www.histology.to). Go to
"Peggy's Links" and select the section on images.

Best regards,
Steven Slap
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: James Martin :      James.S.Martin-at-williams.edu
Date: Tue, 30 Sep 1997 14:09:03 -0400 (EDT)
Subject: thanks for histo websites

Contents Retrieved from Microscopy Listserver Archives
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Thank you to those who have responded to my inquiry after websites for
images of histological samples. I found what I needed.

James Martin
Williamstown Art Conservation Center







From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Tue, 30 Sep 1997 11:02:51 -0700
Subject: substance P TEM

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Some years ago our lab studied substance P and did some immuno-EM. There is
a nicely detailed experimental procedure in this paper: "A substanceP-like
peptide in bullfrog autonomic nerve terminals: anatomy biochemistry and
physiology," C.W.Bowers, L.Y. Jan & Y.N. Jan, Neuroscience, vol 19, No 1,
pp343-356, 1986.
Good luck!
Larry D. Ackerman (415) 476-8751
Howard Hughes Medical Institute FAX (415) 476-5774
UCSF, Box 0724, Rm U426
533 Parnassus Ave. mishot-at-itsa.ucsf.edu
San Francisco, CA 94143





From: edelmare-at-casmail.muohio.edu
Date: Tue, 30 Sep 1997 15:05:32 -0500
Subject: Espon Stylus Photo: Post script level 2?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., with all the positive comments about the Epson Stylus Photo
printer, I have one more question: Since the postscript level 2
emmulation for the Stylus photo adds ~ 20% to the cost of the printer
is it worth it? Every other printer I have has postscript
capabilities and we generally use it (even though we're Intel PC /
Windows based, not Macintosh) any opinions would be great! Thanks.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu


"640K ought to be enough for anybody."
-- Bill Gates, 1981




From: Kirk J C Czymmek :      kirk-at-udel.edu
Date: Tue, 30 Sep 1997 15:38:56 -0400 (EDT)
Subject: Re: WWW sites for histology photos, blood

Contents Retrieved from Microscopy Listserver Archives
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Dear James,

Try http://www.udel.edu/Biology/Wags/histopage/histopage.htm

This WWW site has hundreds of histology images, including several related
to blood samples.

Best Regards,

Kirk J. Czymmek
University of Delaware
kirk-at-udel.edu

On Tue, 30 Sep 1997, James Martin wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Can anyone steer me to a website that has photomicrographs of histological
} samples; in particular, blood samples?
}
} Thanks.
}
} James Martin
} Williamstown Art Conservation Center
}
}
}





From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 30 Sep 97 16:24:04 -0500
Subject: Flat embedding molds

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Martin Bartels wrote:
================================================
I'm just beginning to use LR white as embedding medium for TEM- immuno-
cytochemistry. For previous embeddings of plant root segments for
conventional TEM research with SPURRs resin, I used flat embedding mold of
silicon rubber for simple orientation of the segments. Is it possible to use
flat embedding mold also with LR white and how is it possible to avoid
contact with oxygen during polymerization? What kind of molds are otherwise
most suitable for LR white?
==================================================
One can use flat UV transparent silicone molds for this type of work.
However, since the (transparent) silcone, in order to be UV transparent,
does not have any of the additives normally incorporated to provide chemical
resistance, don't expect long lifetimes, some people report being able to
use a cavity (with L. R. White(TM) for example) only once or twice. The
problem with oxygen exposure is easily "solved" by over filling the cavity
with resin slightly, so there is a positive miniscus, and then placing
another identical transparent mold on top, flat side down onto the over-
filled cavities. Capillary action ensures that there is a quite adequate
seal against the presence of oxygen.

Such molds can be purchased at the main suppliers of accessories of
consumables for microscopy laboraotries including SPI, full details about
which can be found on the SPI website, given below. These molds do not all
"come out of the same source" and are not all the same, so don't assume the
result experienced from one brand would be the same for all other brands.

Disclaimer: SPI Supplies manufactures transparent silicone molds for this
application and we would obviously have an interest in seeing more people
using these transparent molds.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 1 Oct 1997 08:30:54 GMT+1200
Subject: High Temp Resin

Contents Retrieved from Microscopy Listserver Archives
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Hi

Does anyone know of a transparent embedding resin which can be used
up to 300 deg C? For holding mineral chips in a hot-stage.

I'd appreciate an email or fax address of any suitable suppliers.

thanks

Ritchie

Ritchie Sims phone: 64 9 3737599 ext 7713
Department of Geology fax: 64 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: ALLEN-at-aaem.amc.anl.gov (Charles W. Allen)
Date: Tue, 30 Sep 1997 16:42:10 -0600
Subject: Spectroscopy Seminar Nov 14

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"Electron, X-ray and Ion Spectroscopies-A Primer", an all day educational
seminar, will be presented Friday, November 14, 1997, at Argonne National
Laboratory. Advance registration is required. For more information contact
Chas. Allen at allen-at-aaem.amc.anl.gov or Anita Brandes at
g10809-at-email.mot.com including your complete mailing address. We will send
you information and a registration form. Registration fee is $30 ($10 for
students) which includes lunch and refreshments at breaks.


========================================
Charles W. Allen
Electron Microscopy Center-HVEM-Tandem Facility
MSD 212/E211
Argonne National Laboratory
Argonne. IL 60439 USA

Email:allen-at-aaem.amc.anl.gov
Tel: 630-252-4157 Fax:630-252-4798
(Note: On August 3,1996, Area Code changed
from 708 to 630)
========================================






From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Tue, 30 Sep 1997 15:17:10 -0700 (PDT)
Subject: and TOTO too

Contents Retrieved from Microscopy Listserver Archives
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Boarders,

I'm trying to find a protocol that describes the TOTO or PATOTO
(not potato!) technique for fixing plant specimens for SEM. I've tried
doing a literature search and all I get are references to Dorothy and Oz,
just kidding. I've read about the procedure but I cannot find a good
descriptive protocol.
Thanks for helping with this.

I'll get that pretty picture, and it's little dog, too.

Hoping a house doesn't fall on you,



Paula = )

Paula Sicurello
UC Berkeley
Electron Microscope Lab
psic-at-uclink4.berkeley.edu






From: Alwyn Eades :      eades-at-uimrl7.mrl.uiuc.edu
Date: Tue, 30 Sep 1997 17:22:29 -0500
Subject: Change of address

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To everyone with an e-mail address in my computer (sorry if you have this
information already):


ALWYN EADES

(full name: John Alwyn Eades)

IS MOVING TO LEHIGH UNIVERSITY IN OCTOBER 1997
(moving in about ten days)

Present address

Materials Research Laboratory
104 S Goodwin
Urbana
Illinois 61801-2985

is moving to

Department of Materials Science and Engineering
Lehigh University
Whitaker Laboratory
5 East Packer Avenue
Bethlehem
PA 18015-3195

610 758 4231
610 758 4244 FAX
jae5-at-lehigh.edu not activated yet.
**
Alwyn Eades Center for Microanalysis of Materials
University of Illinois at Urbana-Champaign
Phone 217 333 8396 Fax 217 244 2278
eades-at-uimrl7.mrl.uiuc.edu (NB those are letter l not ones)

In mid-October this year (1997), I will be moving to Lehigh. The new
address will be:

Department of Materials Science and Engineering
Lehigh University
Whitaker Laboratory
5 East Packer Avenue
Bethlehem, PA 18015-3195

Phone 610 758-4231. Fax 610 758-4244
E-mail jae5-at-lehigh.edu

Do not use these new numbers until mid-October.
**





From: Mike Boucher :      Mike.Boucher-at-serratia.isd.net (by way of Nestor J.
Date: Tue, 30 Sep 1997 18:20:52 -0500
Subject: Re: correctionlens design

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Peter:
There is a more serious problem which I am sure the Zeiss and Leitz
people will quickly point out. That is that the older series of
objectives had to be used with a corresponding ocular to reduce
optical aberrations, etc. in the objectives, giving you that high
optical quality. Really old scopes had a way of adjusting the tube
length, but that is the least of your problems in a more modern
scope when you switch to another manufacturer's objectives.
Of course the latest scopes have "infinity corrected" optics, but
I wouldn't switch them either.
If you use them, you will not have as good an image, but it would
give you an image. The best tack would be to find an appropriate
Leitz stand with appropriate oculars. Many older scopes are
available, and without the optics are pretty cheap.

Regards,
Mike

} } dear microscopists,
} }
} } Problem: I do have a excellent Zeiss stand and a complete series of
} } Leitz planapochromatic objectives.
} } However as you probable know Zeiss microscopes have a 160 mm mechanical
} } tubelength whereas teh Leitz objectives are calculated for 170 mm.
} } What kind of correction lens can I use in between the tube so that I can
} } use the Leitz lenses on the Zeiss stand without loose of optical
} } quality?
} } Can anybody give me a good advice .
} }
} } thank you in advance,
} }
} } Pieter Houpt
} }
} } (the Hague ,the Netherlands)
================================================
Michael L. Boucher Sr. mboucher-at-isd.net
13345 Foliage Avenue
Apple Valley, MN 55124-5603 Ph 612-432-8836
================================================






From: Ellen Abercrombie :      eabercro-at-bellsouth.net
Date: Tue, 30 Sep 1997 21:14:55 -0400
Subject: unsubscribe

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