This was discussed in the Safety newsgroup quite some time ago - perhaps early last year. There were a lot of ideas put forward from professionals in the book business. Their archives are at
http://siri.org/mail and at http://list.uvm.edu/archives/safety.html
If you can't find the discussion and would like info on subscribing, let me know. Diana.
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
Anybody out there who can tell me the email address of the University of Moscow, Russia. Dept of Electron optics or maybe physics. Any assistance appreciated Peter Earl Toronto,Canada
Just a quick note on gold, carbon thickness monitors. The gold can be= =20 deposited on the smooth side of Aluminum foil or we use 3-4 mil smoo= th=20 thin sheet for stiffness. It can then be cut to a convenient size ea= sily. ---------- =46rom: philippe.buffat-at-epfl.ch To: microscopy-at-Sparc5.Microscopy.Com; daniele.Laub-at-epfl.ch; =20 fabienne.bobard-at-epfl.ch; gaston.peter-at-epfl.ch; Bernard.Garoni-at-epfl= .ch
Steve Wintonick asked on the way to measure carbon film thickness wit= hout thickness monitor by interference colors on gold or polished brass substrate.
I have a copy of a Balzers report that was given to the TEM users in = the mid 1960s: Electron Microscopical Specimen Preparation Techniques fro= m S. Bohler. There are two tables for thickness estimation during coating with car= bon and SiO2:
Carbon on an evaporated gold film/test glass (thick enough to behave = the "gold color"): approx 150=C5 is orange approx 200=C5 is indigo-red approx 240=C5 is blue approx 350=C5 is blue-green approx 430=C5 is green-silver
SiO2 on an evaporated chromium film/test glass gives a sharp color ch= ange: approx 1000=C5 purple approx 1250=C5 blue
Of course, if you place your sample and the test glass at different distances d and D from the source (of small diameter with respect to = d and D). the thicknesses will be in the ratio D^2/d^2.
Best regards
Philippe Buffat
__________________________________________________________________ Philippe Buffat Ecole Polytechnique Federale de Lausanne (EPFL) Centre Interdepartemental de Microscopie Electronique Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time= ) E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/ ______________________________ Eudora F2.1 __________________________= _
This is a reminder that the Early Registration Deadline for Microscopy and Microanalysis '98 is approaching. This deadline has been extended to June 15, 1998, so be sure to get your registration in by that date to save money! Registration forms are available in the Call for Papers, or on the MSA Web site, www.MSA.Microscopy.com. Information on the Technical Program is also available on the Web site. MSA members will also be receiving the Program Book and a Meeting Information Pamphlet in the mail within the next week.
All indications from exhibit sales and early registration are that Microscopy and Microanalysis '98 will match or exceed the successes of M&M'96 (Minneapolis) and M&M'97 (Cleveland). Hotels in Atlanta are filling up quickly, so be sure to also get your reservations in (hotel reservation forms are also available in the Call for Papers, the Meeting Information Pamphlet, and the Web site).
For general information about the meeting, contact the Meeting Manager at 708-361-6000, or at msa-at-tradeshownet.com
A colleague has asked me to inquire if any listmembers have an SEM that will accommodate a small object about 3.5 inches thick (large Z capability). The colleague works in the Kansas City area, but the object can travel.
Thank you.
James Martin Analytical Services and Research, Williamstown Art Conservation Center Research Scientist, Williams College tel: 413.458-5741 fax: 413.458-2314
The Silicon Electronics Research Lab at Bell Labs, Murray Hill, New Jersey, is looking for a TEM technician. Our research interest is in analytical and High Resolution TEM, mainly of silicon based devices and structures.
In our lab, we have a JEOL 4000 EX and a Philips EM420, and will soon purchase an FEG machine. In addition, we have a Micrion FIB. For sample prep, we have the mainly Gatan equipment (Dual Mill, PIPS).
Recently, the TECHNICIAN who looked over the TEMs and the sample preparation facilities retired. We would like to fill this position again.
We are looking for a person who might be interested in helping us running the TEMs and the sample preparation, preferably someone who is able to do TEM specimen preparation and some investigations on his own? (Or who would be willing to learn.) The work would be rather technical than a scientific ("more like tinkering with equipment").
Interested persons may reply by email to frieder-at-lucent.com.
Hi there, a quick question, has anyone had any experience with mounting a Gatan 622 image intensified TV camera on to a JEOL 4000EX (or FX) that already has a Gatan Imaging Filter installed? We have just installed the GIF and also want to use a TV rate camera at lower mag. We dont really care if it has to be mounted off axis or at a weird angle (we do need to get close enough to the microscope to operate it!!). Thanks.
If no one has experience and/pr it is not possible, does anyone want to buy our 622 TV camera?
Note new Area Code (734)
John Mansfield North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 936-3352 Cellular Phone: (734) 715-2510 (Leaving a phone message at 936-3352 is preferable to 715-2510) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
Go to our Links. Click on references and find international universities (or use browser's search function for "international" or "Demello". That will give you several homepages for Moscow universities. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
} } Anybody out there who can tell me the email address of the University of } Moscow, Russia. } Dept of Electron optics or maybe physics. } Any assistance appreciated } Peter Earl } Toronto,Canada } } }
Please bring this technical position opening to the attention of your colleagues. We will accept applications until the position is filled. Thanks!
Bob Summers Director, Confocal Microscopy and 3D Imaging Laboratory SUNY Buffalo ------------------------------------------
SENIOR RESEARCH SUPPORT SPECIALIST: IMAGING LABORATORY State University of New York at Buffalo
DESCRIPTION OF DUTIES: Manage the daily activities of a campus wide biological imaging resource and associated satellite facilities operated by the School of Medicine and Biomedical Sciences. General areas of usage of the laboratory relate to the preparation of biological images for research, publication and instruction. Duties include instruction of faculty, students and technical personnel in the use of digital and analog imaging equipment including confocal microscopes, molecular imagers and scanners, 35mm slide making equipment, video capture and production equipment, photography equipment and printers. Must be familiar with research standards and practices to maintain quality control and advise users on appropriate protocols. Responsible for daily and long-term maintenance and calibration of equipment. Must hire, train and supervise graduate assistants and assign them to assist users of the imaging resource. Develops and manages budget, maintains financial records of the laboratory and collects user fees from users of the laboratory. Should work closely with Director and Faculty Advisory Committee to establish needs, set priorities and develop policies within the laboratory.
QUALIFICATIONS: MS or MA in Biological Sciences. Research experience that includes microscopic and macroscopic biological imaging, graphics illustration and photography. Preparation of biological specimens for microscopy. Experience in use of computer hardware to include Apple, PC and Unix platforms and operating systems, scanners, imagers and digital microscopes. We desire experience with computer software including PhotoShop, Corel Draw, Canvas, PowerPoint, Persuasion, Desktop Publishing and Word Processing Programs. Experience in installation of software and maintenance of computer hardware. Must have experience with using computer networks. Personal skills should include working with large number of researchers and/or educators to expeditiously handle work requests and to attain customer satisfaction.
SALARY commensurate with qualifications. SUNY Buffalo is an EOAA Employer
FOR INFORMATION OR TO APPLY CONTACT: Dr. Robert G. Summers, 716.829.2911 (phone) or fax credentials to 716.829.2915 or email to: summers-at-confocalsgi.med.buffalo.edu
I'm looking for a software (freeware or shareware) for diffraction patterns analysis.
Thanks !
******************************************** * Paulo C. Soares Jr. * * LaMaV - Laboratory of Vitreous Materials * * Federal University of Sao Carlos * * Sao Carlos - Brazil * ********************************************
There will be a Photomicrograph Exhibit held in conjunction with the 14th International Congress on Electron Microscopy in Cancun this August 31 - September 4. If you are attending the meeting, please consider bringing up to 3 micrographs for inclusion in a Photomicrograph Exhibit. From this exhibit, images will be selected for inclusion in a post conference publication. See details below and if you have questions contact either the meeting headquarters in Mexico City (info below) or myself.
Photomicrograph Exhibition
The 14th ICEM will include a micrograph exhibition. The organizers' intentions are to provide a showcase for outstanding micrographs displaying a combination of art and science.
The exhibit is open to all forms of microscopic imaging. A panel of judges will select entries based on artistic merit for inclusion in a post-congress publication of images. Submitted micrographs much be accompanied by a brief description, not only of their scientific content, but also of their technical aspects. If images have been digitally processed or altered, the digital processing should be described as well. All submitted micrographs will be displayed although entries not regarded by the judges as artistically significant will be excluded from the publication of images. Please read the following rules carefully:
Only registered attendees at the 14th ICEM are eligible to submit micrographs.
Any individual may submit up to three micrographs.
Entries must be 27.5 cm x 35.0 cm, may be mounted vertically or horizontally, and must be affixed to a stiff lightweight support, such as 10 mm foam board. Micrographs may either be flush mounted or have borders so long as the overall dimensions of the entry are 27.5 cm x 35.0 cm.
Entries must be brought to the meeting and mounted on the display boards by the entrant or his/her delegate. Velcro will be provided. Entries must be mounted between 12:00 and 17:00 on Monday, August 31st and those micrographs not selected for publication must be removed between 12:00 and 15:00 on Friday, September 4th. Micrographs remaining on the display boards after then will be discarded. Micrographs selected for publication will not be returned to the entrant and will become the property of the 14th ICEM.
Selected micrographs will be incorporated into a post-congress publication of images. Those selected for inclusion will be announced during the Thursday evening reception.
To enter: Send your name, address, telephone and fax numbers, and email address plus a description of 200 words or less for each entered micrograph (maximum of 3) to: Secretariat 14th International Congress on Electron Microscopy Amsterdam 46-202 Col. Hipodromo Condesa C.P. 06100, Mexico, D.F. MEXICO Phone: (525) 553-4507; Fax (525) 553-4500 email: icem-at-icem.inin.mx
Entry information must be received by August 1, 1998. Entries will be acknowledged promptly. Do not send micrographs, you must bring them or have them brought to the Meeting.
Meeting Office staff will print your description(s) in standard form and prepare them to be mounted along with your micrograph(s) at the Meeting.
___________________________________________________________________________ Barbara Reine, Botany Dept. Box 351330 Univ. of Washington, Seattle, WA 98195-1330 e-mail: reine-at-u.washington.edu; Phone: (206) 543-1955; Fax: (206) 543-3262 ____________________________________________________________________________
Hi, Does any of the listers know any ER specific or Golgi specific antibodies for immuno-gold or confocal staining? Thanks.
Li-Tzu Li Dept. Medical Technology College of Medicine National Taiwan University Taipei, Taiwan Phone: 886-2-2397-0800 ext 6907 Fax: 886-2-2381-7083 Email:ltl-at-ha.mc.ntu.edu.tw
If you have a reasonably quick web connection, why don't you try EMS On-Line at http://cimewww.epfl.ch/ . This does loads of things which also includes image simulation of TEM images. I have found it very useful for indexing SAD patterns recently.
Hope this helps
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
TECHNICAL ASSISTANT: LIGHT AND ELECTRON MICROSCOPY AND X-RAY SPECTROMETRY
This half-time position will oversee the light and electron microscopy facility including scanning and transmission electron microscopes, a laser scanning confocal microscope, an energy dispersive X-ray spectrometer, digital image analysis equipment, diverse sample preparation equipment, and two darkrooms. The facility serves Biology and Geology, as well as Art, Astronomy, Biochemistry, and Neuroscience faculty, research students and teaching labs.
Responsibilities: oversee the day-to-day operation of the facility, including routine maintenance and troubleshooting of instrumentation, ordering supplies, and general laboratory and darkroom maintenance; provide training to faculty and students in specimen preparation, proper use of the various microscopes and detectors, digital image processing, and traditional darkroom techniques; prepare laboratories for specific courses; maintain microscope inventories; assist with other designated instructional tasks. The applicant must have excellent communication skills, provide expertise to people of diverse skills and research interests, be able to set priorities and work without direct supervision, and bring enthusiasm to the job. The position carries the possibility of both additional teaching responsibilities with added compensation and use of the facility for research.
Requirements: Master's degree in science with considerable experience in the areas of electron and confocal microscopy or X-ray microanalysis. Computer literacy (preferably in both the Macintosh and Windows environment) is desirable. Annual starting salary is $17,500 and includes a comprehensive benefits package.
Review of applications will begin July 1 and continue until the position is filled. Forward a letter of application, resume and the names and telephone numbers of three professional references to: Richard Briggs, Department of Biological Sciences, Smith College, Northampton, MA 01063. Smith College is an equal opportunity employer encouraging excellence through diversity.
Hi all. Apologise for the lack of chit chat. I too have been struck by the = influenza virus doing the rounds.=20
Again as part of the ongoing promotion of MSSA and microscopy in SA. We = have a small portable Hitachi SEM available to us which could be used to = do demo's at schools. It is a very old system and imaging is not the best, but it should be = interesting for school students and teachers to see a working SEM.
The best way to promote microscopy and the E.M.unit at universities, is = to involve both, and so by letting a biology student from a university = in the region, for example, demo the SEM, would be the ideal way of = going about having such a visit to a school. The SEM is here in our workshops in JHB, but we can make a plan to get = it around the country. To connect it up takes a 15A 220v Ac mains plug and a water supply. It = will fit on one standard table and fit in the boot of a car.
So if any body would like to assist with the organising of such a visit, = please let me know.
At this stage I would need to know who is interested and which schools = are interested, then we can start to arrange for dates.
Look forward to your responses.
P.S. this system is not for sale.=20 Luc Harmsen=09 Anaspec, South Africa International technical support on microscopy. Tel: +27 (0) 11 476 3455 Fax:+27 (0) 11 476 7290 anaspec-at-icon.co.za
One of the "classic" ER-staining antibodies seems to be anti-BiP (immunoglobulin binding protein).
See for more information and additional references the following paper:
Terasaki M, Reese TS. 1992. Characterization of endoplasmic reticulum by co-localization of BiP and dicarbanocyanine dyes. J. Cell Sci. 101, 315-322.
Scott Gens gens-at-biodec.wustl.edu
On Tue, 2 Jun 1998, Li-Tzu Li wrote: } Hi, } Does any of the listers know any ER specific or Golgi specific } antibodies for immuno-gold or confocal staining? Thanks.
with SMTP (Eudora Internet Mail Server 1.2); Tue, 2 Jun 1998 11:33:09 -0500 Message-Id: {v0151010cb199d6d02266-at-[137.99.40.57]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Does anyone know where I can get a product called "Coat-quick G"? It is mentioned in a protocol for coating grids which are to be subjected to resin etching and immuno labelling. I assume it is to aid adhesion of the sections to the grid during these procedures. If you do not have a source for this product, perhaps you know of a similar one. Thanks.
Marie
Dr. Marie E. Cantino Dept. of Physiology and Neurobiology, U-131 University of Connecticut Storrs, CT 06269 Ph: 860-486-3588 Fax: 860-486-1936
--------------------------------------------------------- The Electron Microscopy Laboratory at New Mexico State University has an open position for an Electron Microscopy Specialist. The laboratory is a multi-user facility that provides TEM, SEM, and light microscopy services for the university research community and a few external organizations.. Qualifications: MS Degree minimum, Ph.D. Degree desirable, with at least three years of electron microscopy experience. The preferred candidate will be skilled in transmission electron microscopy and immunohistochemical techniques as applied to the study of plant and animal (nervous system; cell culture) cells. Knowledge of fluorescence microscopy, SEM, digital image analysis, and the use of networks in computing are assets. He or she also should be experienced at teaching microscopy fundamentals and in managing a multiuser teaching and research laboratory. The ability to work well with research faculty, other EML staff and students is essential. Duties: operation and routine maintenance of microscopes and associated equipment, sample preparation, coordination of projects, billing, course instruction, supervision of student research projects, some educational outreach activities, technical support for EML components of sponsored grants, training of laboratory users. Salary: $33,544-$40,500 DOE plus 26% fringe benefits. Review of applications will begin June 19 and continue until the position is filled. Applications should include a resume, a letter of interest and three letters of reference.
Apply to: Dr. Reed Dasenbrock Associate Dean/Director Arts and Sciences Research Center New Mexico State University MSC RC, Box 30001 Las Cruces, NM 88003 rdasenbr-at-nmsu.edu
For more information about the NMSU EML access: http://www.nmsu.edu/Research/artsci/public_html/eml/index.html#staff
Randy Tindall Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003
Position Available: 2-year postdoctoral fellowship in plant pathology employing histology and electron microscopy.
A research position is available for a postdoctoral fellow to work in cereal pathology under the NSERC Visiting Fellowship program.
The research will be conducted in the Crop Science section at the Agriculture and Agri-Food Canada Research Centre in Lethbridge, Alberta, Canada. The successful applicant for this position would be working as part of a research team with Dr. R. L. Conner (cereal pathologist) and E.G. Kokko (electron microscopist).
The study will compare the development and spread of different black point fungi in resistant and susceptible wheats. The investigation will involve a histological examination of wheat tissue using light and electron microscopy. The overall objective is to clarify the factors involved in grain discolouration caused by this disease and the mechanisms controlling disease resistance.
The candidate for this position must have a PhD in Botany, Plant Science or Plant Pathology from a recognized University. Previous experience in light microscopy as demonstrated in research publications is essential. A strong background in plant biochemistry is also required. Familiarity with procedures used in scanning and transmission electron microscopy would also be an asset.
The appointment is for a two year period and salary will be according to current rates for the NSERC Visiting Fellowship Program. Current starting salary is $35,184.00 (Canadian dollars).
The Lethbridge Research Centre is looking for candidates for this position. Applicants should submit a brief resume describing their education background, research activities, and a list of publications along with at least two professional references. Only those who are considered qualified will be given further consideration and contacted personally.
For further details, contact:
Eric Kokko Agriculture and Agri-Food Canada Lethbridge Research Centre P.O. Box 3000, Lethbridge, Alberta CANADA T1J 4B1
Dr. Cantino, Coat Quick "G" is sold by Electron Microscopy Sciences, Fort Washington, PA, 800-523-5874 (EMS Catalog XII, p.68). -------------------------------------------------------- Winston W Wiggins, Supr. 6/2/98 3:41:06 PM CRC-Electron Microscopy Lab. Ofc:704/355-1267 Carolinas Medical Center Fax:704/355-7648 P.O. Box 32861 Lab:704/355-7220 Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org --------------------------------------------------------
Fellow Microscopists, Recently our lab purchased a second TEM for asbestos analysis. Can anyone give me some advice on screen design for a JEOL 100CX specifically used for asbestos analysis? Currently we are using a Hitachi 7000 with an (11cm. max) concentric circle design. The only problem I find with this is trying to determine the width/diameter of a fiber for a weight % analysis. The smallest division is 0.25 cm., which makes it very difficult to measure individual fibers at a mag of 20,000X. Any input would be greatly appreciated.
Cheers,
Kevin MacKinnon Micro Analytical Laboratories, Inc. Emeryville, CA 94608 (510) 653-0824
_____________________________________________________________________ You don't need to buy Internet access to use free Internet e-mail. Get completely free e-mail from Juno at http://www.juno.com Or call Juno at (800) 654-JUNO [654-5866]
Hello, my name is Jeff Pierce. I am a microbiologist working for Zeneca in Wilmington, Delaware and have been given an assignment to photograph ( by SEM ) basic pictures of bacteria, fungi and yeasts to be then colorized and used as photographs in technical literature. ( Zeneca manufactures specialty biocides for industrial applications such as coatings, polymers, pigments, etc. ) Anyway, I did a lot of TEM work at Penn State and only really dabbled in SEM. To the question at hand: does anyone have any neat ideas regarding making interesting SEM pictures of bacteria, yeats or fungi? I used to simply filter cultures thru a nucleopore membrane and photograph the membrane with the bugs trapped on the surface. However, the background ( the filter ) takes away from the bacteria. Does anyone have any suggestions that may help? Also, where can I find a current SEM dehydration/sample prep technique for these types of specimens? Any help/comments/suggestions would be greatly appreciated. Thanks in advance, -Jeff Pierce.
I got a few responses to my questions on the "megapixel" consumer digital cameras vs. scientific digital cameras. Many issues are still fuzzy, but since there was some interest in the responses I have paraphrased them below.
But first - would the person from Kodak who responded please re-send your E-mail? (I accidentally deleted it)
RESPONSES:
1. The grade of CCD chip used can differ greatly between the lower and higher priced models. They are graded on their defects (either cluster or column/row defects), which affect the ability to record data. Chips with few or no defects are rare and reserved for the highest scientific grade cameras, from manufacturers like Princeton Instruments and Xilliger [and possibly others not mentioned by the respondent]. A single-chip B/W camera in this grade might cost $40k or more for 1kx1k resolution. The application dictates the need - intensity and color analyses being one application where high quality chips would be desired. The respondent also mentions that Princeton Instruments (no connection) has further information on chip defects.
2. Some references for reviews of digital cameras: Popular Photography (Dec. 1997), PEI - Photo Electronic Imaging (May 1998) www.peimag.com, PC Magazine (February 1998). This person chose the Olympus D-600L after receiving quotes from National Graphics Supply 800-223-7130, B&H 800-947-5516, and Wolf Camera.
3. A recommendation for the MicroLumina (couldn't tell if from a vendor or not??) with 3kx2k resolution, 36 bit color, and standard Nikon 35mm lenses or microscope adapter. Web page http://www.electroimage.com
4. A response from DVC company recommending their web site Q&A/FAQ section on digital cameras at http://members.aol.com/dvcco Noted that camera differences have to do with dynamic range/ bit depth and speed.
5. Responses from other vendors who simply left their phone numbers and offered to discuss the issue.
If anyone wants specific details or vendor phone numbers please E-mial me privately.
Karen
} } ORIGINAL QUESTION: } ------------------------------------------------- } To the digital die-hards out there: } } I know there have been MANY threads lately on digital cameras, which } have been very informative. But there is such a boom going on out } there! Does anyone have a good handle on the differences between the } "megapixel" models which are marketed for consumer/professional } photography markets vs. the scientific models discussed on this list?? } } Specifically, a colleague recently challenged me as to why I would want } to "waste" $6000 on a Minolta RD-175 when I could get even better } resolution from some of the Olympus, Kodak, Konica, Canon, etc etc. } products out there for $1000 or less. After some web site searching, it } seems like there might be an issue of true color representation (1CCD } vs. 3CCD's) and perhaps lens mounts, auto-white balance w/o manual } override, compression, or other things. But I'm not sure per particular } model. Anyone want to get into some of these details?? } } NOTE: my needs are for Both hand-held close up work and possibly } microscope-mount, to be used in subsequent image analysis of colors & } densities as well as morphology. Low-light sensitivity not essential. } } Some of the suggested models were: } } High End: } Minolta RD-175 } Kodak DCS 420, 410, and 460 [anyone know a good vendor in Minnesota?] } Polaroid DMC2000 } } Lower End or Unknown: } Sony DKCST5 [again, any vendors in Twin Cities area?] } Kodak DC120 or MDS120 } Konica Q-M100 } Olympus D-600L } Canon EOS DCS 1 } Pixera Professional [I have this] } many others I haven't looked up yet } } I hate to beat a dead horse, but this is more like cutting up a } starfish. The issues just seem to multiply! } Any input appreciated, } Karen
-- Karen Zaruba, kszaruba-at-mmm.com BioMaterials Technology Center 3M Center Bldg. 270-1S-01 St. Paul, MN 55144
*The opinions above are my own, not necessarily my employer's*
FEI Company has the following position available at their Mahwah, NJ location:
FEI Company Job Opportunity Mahwah, NJ
National Technical Support Specialist - SEM
Summary: Provide technical support to ensure customer satisfaction and profitability. Provide first line support to both Philips customer service engineers and customers via the technical assistance center.
Duties and Responsibilities: * Oversees policies and processes which motivate and develop talent to provide technical support with the product group. * Acts as a liaison between supply center and field personnel to resolve technical problems. * Provide means of Technical Assistance Center (TAC) support to assist in customer telephone fix or call avoidance. * Evaluates field Customer Service Engineer's (CSE) for training requirements and makes recommendations to National Customer Service Manager. * Writes service bulletins to field as needed. Interfaces with bulletin boards as needed. * Prepares and conducts service training courses as required. * Reviews and evaluates all new products and documentation. Monitors new product installation. * Develops corrective action plans to resolve complex/long-term product reliability problems through use of MFG-Pro data analysis. * Assists to completion all field installations, upgrades and escalations via telephone daily. * Establishes initial stock levels for spare parts. * Provides technical assistance via telephone to all X-Ray personnel including review of orders as required. * Provides on-site technical assistance to CSE's when required. * Ensures the adherence to company policy, suitability of work instructions, implementation of corrective action and maintenance/accessibility/retention of quality documentation. * Ensure that ISO 9000 directives that pertain to this area of responsibility are fully complied with.
Required Knowledge, Skills and Experience: * BS/BA or AAS Electronic Technology or equivalent. * Must have excellent mechanical and electronic aptitude * 5+ years experience with SEM's (scanning electron microscopes) * Must possess excellent communication and problem solving skills * Must have a working knowledge of software operating system.
Interested candidates should FAX or email resume to Lisa Olivia FAX: 503.640.7509 email: lolivia-at-feico.com
Lisa Olivia Recruiter FEI Company PH) 503.844.2601 FAX) 503.640.7509 email: lolivia-at-feico.com
#03171-Lab Specialist Senior - CISAT Assists in all aspects of procedures required for the prepapration of tissue for immunohistochemical analysis (whole mounts as well as sections), fluorescent and light microscopy, transmission and/or scanning electron microscopy. Competitive candidates must possess knowledge of techniques for immunological staining, sectioning, mounting tissue, evaluating sections and taking and developing publicaiton quality photomicrographs. Applicant must possess demonstrated ability to operate: ultramicrotome, vibratome, cryostat, and other TEM/SEM equipment. Applicant will be responsible for maintenance of specimen records, chemical supplies, reagents, etc in the histological laboratory and for independently modifying lab procedures to obtain optimal results. Interpretation of findings and ability to direct the work of others also necessary. Four year degree in Biology or related field supplemented by post-graduate experience in microbiology and/or neuroscience preferred. Salary range: $24,337-37,995.
Send resume and all correspondence to:
Brenda M. Ryals, Ph.D Professor Communication Sciences and Disorders James Madison University Harrisonburg, VA 22807 ryalsbm-at-jmu.edu
We are looking for a used TEM (in reasonable working condition) that anyone might have for sale or donation. The machine will be used for routine imaging. Please contact me offline if you have any information.
Thanks,
Mohan Kalyanaraman
Sr. Staff Material Scientist Mobil Technology Company PO Box 480 Paulsboro, NJ 08066 mohan_kalyanaraman-at-email.mobil.com
This message is in MIME format. The first part should be readable text, while the remaining parts are likely unreadable without MIME-aware tools. Send mail to mime-at-docserver.cac.washington.edu for more info.
I, Dr Yogis Naidoo a research officer in the Electron Microscope Unit at University of Durban-Westville, South Africa am interested in a post doctoral research position. I will be able to provide my own funding. My field of specialisation is ultrastructure and cytochemistry salt glands of halophtyic plants. My current project focuses on investigating the ultrastructure and cytochemistry of salt glands of halophytic grasses along the South Farican coastline. I am also interested in glands of other related plants. I will be willing to give lectures on plant pathology.
Thank you Yours sincerely Y. Naidoo Electron Microscope Unit University of Durban-Westville Private Bag X54001 Durban 4000 Tel: (031) 2044360 Fax: (031) 2044809
Would anyone be able to recommend us on what diamond knife angle would be a better choice for cutting polymer (polymer-metal/glass fiber composite)? 45 degree or 35 degree or doesn't matter?
I was told by Diatome that 35 degree knife would be a better choice to cut polymers, i.e., it reduces the curling problem. But we are currently using the 45 degree knife. Would it be wise to resharpen the one we have or buy a new one that is 35 degree?
"Analytical Electron Microscopy of Oxynitride Glass Ceramics"
A post-doctoral position is available in the Division for Microscopy= and Microanalysis at Chalmers University of Technology within the framework= of the Training and Mobility of Researchers (TMR) Programme. Qualified candidates should have a Ph.D. in materials science, physics or chemistry, and a documented background in analytical electron microscopy. It= will be considered an advantage if he or she has some experimental experience= of any of the following techniques: electron energy loss spectroscopy= (EELS), convergent beam electron diffraction (CBED) or high resolution lattice imaging. The applicant must be a national of a Member State of the European Community. The starting date of the appointment may be= discussed, and the appointment may last up to 16 months.
The position is funded by the TMR networks research project "Structure= and Properties of New M-Si-Al-O-N Oxynitride Glass Ceramics (M=3DY,Ln)"= which is a collaboration between seven partners in the United Kingdom, Ireland, =46rance, Sweden and Belgium. The collaboration within the established network will give the post-doctoral fellow good contacts with a number= of research groups in Europe and a good knowledge of a variety of preparative / analytical / property measurement techniques relevant to the evaluation of new ceramic materials as well as other materials outside the field= of ceramics.
The aim of the research project is to determine crystal structures= and properties of currently uncharacterised new glass-ceramic phases= in yttrium and rare earth sialon systems. These materials are potential refractory grain boundary phases in Si3N4-based ceramics, and have an interest= also as refractory surface coatings (glazes) and as interface materials in nitride-oxide and nitride-metal joints.
The analytical transmission electron microscopy work carried out= at Chalmers will focus on the chemistry and structure of glasses and= their crystallisation products, and on microstructural development during nucleation and growth processes. Microanalytical work on glass ceramics subjected to creep testing and oxidation / corrosion experiments= may also be carried out.
The major part of the microscopy work will be carried out on a Philips CM200 Supertwin transmission electron microscope (TEM) with a field emission gun (FEG) and surrounding interactive instrumentation. = The FEG TEM is equipped with the Gatan imaging filter (GIF) which produces= energy filtered electron images and diffraction patterns as well as electron energy loss spectra, a Gatan off-axis CCD camera for high resolution imaging and a Link Isis energy dispersive X-ray (EDX) system for qualitative and quantitative elemental analysis including the light elements. There are also other electron microscopes in the laboratory, e.g. a Jeol 2000-FX TEM/STEM/SEM and a CamScan SEM.
=46or further details, please contact: Associate Professor Lena Falk, Department of Experimental Physics, Chalmers University of Technology, SE-412 96 G=F6teborg, Sweden e-mail: lklfalk-at-fy.chalmers.se; fax: +46 31 772 3224; phone: +46= 31 772 3321
Does anyone have a really goood fixation for lm of very hard seeds. We would like to section buckthorn seeds.
Debbie Lietz Electron Microscopy Suite Department of Biology Trent University Peterborough, Ontario K9J 7B8 Telephone: (705)748-1486 Fax: (705) 748-1205 email: dlietz-at-trentu.ca
There has been a lot of discussion recently about substituting one DP oil for another. Here is a summary of the properties of some of the common brands of oil: MM VP BT HV
MM = Avg molecular mass; VP = vapor pressure at 20 C (Pa); BT = boiling temperature (deg C) at 100 Pa (typical DP boiler pressure); HV = heat of vaporization (kJ/mole)
These values are probably not accurate to a high degree, because it is very difficult to measure the physical properties of very viscous, low vapor pressure fluids such as these; however, they should be good enough to give you an idea of the relative characteristics of the various fluids.
In general, if you are going to substitute one oil for another, the two should be as similar as possible with regard to boiling temperature and heat of vaporization. Rough values of boiling temps are given above; however, I could not obtain values for the heats of vaporization that I could feel confident enough about to publish in my book. The values I did come up with are given above, and are only VERY ROUGH. Most manufacturers do not give heat of vaporization data, even though it is a very important property. It is possible to calculate it from data giving variation in vapor pressure with temperature (see VM in EM p 181) but the vapor pressure data are usually not precise enough to yield results one can really trust.
More details on diffusion pump fluids can be found in Sect. 5.4 of my book 'Vacuum Methods in Electron Microscopy' (http://www.2spi.com/catalog/books/book48.html ; http://www.bookshop.co.uk/portland/)
Incidentally; concerning the character of Lion-S Oil, two weeks ago I wrote to the Lion Oil Company requesting information about this oil, but have not yet received a reply.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
As I have mentioned on several previous occasions, it is quite possible to clean most parts of the vacuum systems of EMs and other common laboratory apparatus WITHOUT the use of organic solvents.
The use of grease-based polishing compounds, such as are commonly used throughout the field, makes no sense at all. A major reason for cleaning parts in the first place is to get organic contaminants such as pump oils and greases, fingerprints,etc., off them. Why then do people go about doing so by coating the parts with a greasy polishing compound? This is like taking a bath in a mud puddle! Not only does the greasy polishing compound thoroughly coat the parts, but it gets embedded in every crack and crevice in them. To remove it you then have to wash the parts repeatedly in organic solvents which are expensive to purchase and also to dispose of.
Methods for cleaning vacuum parts without using greas-based polishing compounds are discussed in some detail in Sect. 2.10.4c (p. 69) of my book "Vacuum Methods in Electron Microscopy" (for info see: http://www.2spi.com/catalog/books/book48.html and http://www.bookshop.co.uk/portland/).
Since I have outlined these methods generally on this list server on a couple of previous occasions, I will not do so again. However, I would like to mention a very useful cleaning agent that is not discussed there: Tilex Soap Scum Remover. This is a combination of diethylene glycol butyl ether (a very effective solvent) and powerful detergents. It is usually possible to clean most parts simply by scrubbing them with TSSR (treating ultrasonically if desired), rinsing with hot running tap water (again treating ultrasonically if desired), rinsing with reagent grade isopropyl alcohol, and drying with a gas blaster. Check my book for more details and other useful cleaning materials.
A word of caution: the pole pieces of magnetic lenses are usually made of a soft iron alloy which corrodes rather easily, and so should not be cleaned with water; in fact, they should not be cleaned at all unless it is absolutely necessary to do so.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
Wil Bigelow writes ... } } There has been a lot of discussion recently about } substituting one DP oil for another. Here is a summary of } the properties of some of the common brands of oil ...
First of all, thanx for the summary. However, I wish you would have included one other factor ... that being, whether the oil is silicone or hydrocarbon based. My impression is EM manufacturers do not suggest silicone based oils because the contamination is difficult to remove and is non-conductive. It ^has^ been a long time since having researched this issue ... I'll expect to be corrected/enlightened if I'm wrong ... it may even be possible you've listed only hydrocarbon based DP oils ...
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - http://darkwing.uoregon.edu/~mshaf/ Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu or mshaf-at-oregon.uoregon.edu
at National University of Ireland, Galway ------------------------------------------------------- Papers, Posters, Workshop, Trade Exhibition -------------------------------------------------------
Guest Speakers
Professor Brigid Heywood, Keele University, England
"Bio-inspired strategies for materials fabrication"
Professor Tom Fleming, Southhampton, England
"Biological application of confocal microscopy to the study of early mammalian development"
Student prizes for papers and posters in Materials sciences and Biological sciences
------------------------------------------------------ Further details from Professor Jean Folan-Curran, Department of Anatomy, National University of Ireland, Galway. Tel: (+353) 091-750305 Fax: (+353) 091-750520 mailto:j.folan-curran-at-ucg.ie
Rather than make my way over to the library, I thought I'd poll the expertise of the group. How could I tell if some intracellular blobs are primarily lipid, primarily protein, or both/neither? Not a real analysis, but just in general. You know the things - "apears to be a lipid-like structure". This would be in plant material embedded in resin (Spurr or LX-112), and the spores are too small to see well with LM, so it rules out doing PAS at that level.
Any suggestions gratefully accepted!
Mahalo, Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I have been freezing copepods (small planktonic crustaceans) by plunging into freezing propane, and then cryosubstituting with 2% OsO4 in anhydrous acetone (switching to methanol next week). The holder I have jury-rigged I think is not an adequate sink to slow the warming, and so I would like to have our shop mill out an aluminum block for me. I know there have been at least a couple of papers giving the dimensions of such a block and it's thermal properties, but I can't lay my hands on them at the moment. I would appreciate hearing from anyone who can suggest a reasonable start.
Also, I would like to hear from anyone with experience with ultrarapid freezing of marine (wet and salty) organisms, both plant and animal. Any particular problems or successes?
Thanks, Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I am looking for prepared TEM grids for a variety of different samples (vertebrate tissues, invertebrate, plants, bacteria, viruses) that anyone may be willing to part with for the cost of shipping. I currently operate a functional Zeiss 9A TEM which I demonstrate to our undergrad. students but we are still several months away from having the ability to prepare our own grids. Thanks in advance for your help.
Mike
Michael A. Palladino, Ph.D. Instructor of Biology Brookdale Community College Lincroft, NJ 07738 Voice: (732) 224-2871 E-mail: mpalladino-at-brookdale.cc.nj.us
I am looking for prepared TEM grids for a variety of different samples (vertebrate tissues, invertebrate, plants, bacteria, viruses) that anyone may be willing to part with for the cost of shipping. I currently operate a functional Zeiss 9A TEM which I demonstrate to our undergrad. students but we are still several months away from having the ability to prepare our own grids. Thanks in advance for your help.
Mike
Michael A. Palladino, Ph.D. Instructor of Biology Brookdale Community College Lincroft, NJ 07738 Voice: (732) 224-2871 E-mail: mpalladino-at-brookdale.cc.nj.us
Hi to all fellow microscopists, I have appealed via a colleague before about this problem and thought I had solved it, but to no avail. We are looking for anyone selling parts from an Hitachi H-600 TEM. We need to replace the gun and cable and as yet have not had a promising response. The agents will not even give a price, as I believe this part is not stocked anymore.HELP from anyone who has information that I can use Bruce Dando
} Hello, all } } Rather than make my way over to the library, I thought I'd poll the } expertise of the group. How could I tell if some intracellular blobs are primarily lipid, primarily protein, or both/neither? Not a real analysis, but just in general. You know the things - "apears to be a lipid-like structure". This would be in plant material embedded
Tina:
In animal material lipid droplets are not surrounded by a membrane while protein and carbohydrate granules are. See the chapter on Adipose tissue in almost any edition of "A Textbook of Histology" by Bloom and Fawcett for an micrograph of this (taken by one of my mentors, Eunice Wood).
Geoff -- *************************************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane Piscataway, NJ 08854 voice: (732)-235-4583; fax -4029 e-mail: mcauliff-at-umdnj.edu ***************************************************************
Some time ago there was a thread regarding using a gold coin as a replacement for the manufacturer's gold target on sputtering units.
If there is anyone familiar with the topic, I could use a little help. Standard gold coins are 99.9% gold, can I assume this is pure enough? Do the coins need to be smoothed out? And can they be soldered in, or is brazing prefered?
Any additional hints would be greatly appreciated.
Daraporn Arayasantiparb wrote: =============================================== Would anyone be able to recommend us on what diamond knife angle would be a better choice for cutting polymer (polymer-metal/glass fiber composite)? 45 degree or 35 degree or doesn't matter?
I was told by Diatome that 35 degree knife would be a better choice to cut polymers, i.e., it reduces the curling problem. But we are currently using the 45 degree knife. Would it be wise to resharpen the one we have or buy a new one that is 35 degree? ================================================ We have cut samples of this type for more years than I would care to admit. You did not mention the metal but let me assume it is something not too hard like aluminum or copper. The hardness of the metal and its size is the single most important factor in assessing possibilities of sectioning this particular kind of system.
It is a fact that the smaller knife angle does give all kinds of advantages, another one being that it will also be easier and faster to get "acceptable sections" however you want to measure "acceptable".
But what is left unsaid is the accelerated wear rate of the knife edge. It has at least been our own experience that a 35 deg. relative to 45 deg. will "wear out" far faster, perhaps a factor of ten faster. On the other hand, if you are experienced with the microtomy of these kinds of samples, or once you become experienced, you can overcome this issue to the point that you can (usually) obtain samples that are quite acceptable fairly quickly with the 45 deg. edge. But there will always be some samples at the margin where this is not going to be the case. There will be some samples that just can not be cut at 45 deg. but which can be cut at 35 deg. Now we love these kinds of samples since they can consume a lot of diamond knives in a short period of time.
We also find that this kind of sample is more easily cut at RT than cryo. Those at the "margin" can't be cut cryo at all.
We use in our own laboratory our own SPI Materials Science Diamond Knife, 45 deg. edge for this type of sample. Depending on the hardness of the metal particles and the adhesion of the filler to matrix, there can be some pull out. But the sections are basically usable and the knife does not wear out in an afternoon. The hardness of the metal will determine how fast there is developed a pattern of "knife marks" at the edge. The next most important factor is the diameter of the glass fibers.
So the real answer to your question is a "we don't know". But if you have the 45 deg. knife anyhow, it would seem that you should not assume the worst case scenario, have it sharpened and see what you can do with it.
Disclaimer: SPI has offered a special "materials science diamond knife" with standard 45 deg. angle for some years so we have a vested interest in seeing more use of diamond knives for this kind of sample. And, yes, we are a competitor of Diatome.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
Dear Listers, Wil Bigelow recently posted a message on the properties of various DP oils. We are using a fluorinated oil called Krytox 1525, which was not one of the oils discussed. Wil, do you (or anyone else) know the MM, VP, BT & HV of this oil? TIA. Yours, Bill Tivol
Early versions of the JEOL 100CX TEM use 16.2volt and 8.1volt mercury batteries as reference voltage source. There is also a 29.7volt mercury battery in the objective lens circuit.
Now that mercury batteries are no longer available, can anyone tell me if there are any suitable substitutes available, preferably in the UK?
Regards,
Bob Phillips
MicroServiS
11 Grafton Close, St. Ives, Huntingdon, http://dspace.dial.pipex.com/microservis/ Cambs., PE17 6DL
} } Some time ago there was a thread regarding using a gold coin as a } replacement for the manufacturer's gold target on sputtering units. } } If there is anyone familiar with the topic, I could use a little help. } Standard gold coins are 99.9% gold, can I assume this is pure enough? Do } the coins need to be smoothed out? And can they be soldered in, or is } brazing prefered? }
Any half way competent high street jeweller can hammer out a pure solid gold target for your sputter coater.
Ours are 0.5 mm thick, 70 mm diameter.
They can start with a gold coin, or will make it out of any weight of pure gold you specify.
Once it is worn through, you take the remnant back to the jeweller and ask him to make it up back to the original weight.
It is never wasted........
***************************************************** Mel Dickson, Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
I've been asked to put out a call for help in sourcing a cheap (as possible) X100 air spaced objective with a minimal working distance of 0.4mm but preferably much longer (ideal 4mm) and a high NA greater than 0.65 (double would be good).
This seems a lot to ask but i'm sure someone out there can detail a best fit to these requirements.
Many thanks
Dr. Mark Osborne
Department of Chemistry Uni of Cambridge Lensfield Rd UK CB2 1EW
All you need to make a piece of metal the target of your coater is to fix=
it in place such that it makes a good contact with the power source. =
Provided the base unit upon which it is mounted is aluminium you will not=
have any sputtering from the base. Hammering the coin flat will give you= a bigger source area of course.
Many targets are clipped in place, a poor contact may be improved with th= e use of conducting paste the same as you would use to fix a specimen to to=
stub.
The material should be more than adequate for Tungsten SEM applications.
For the first sputter do not use a specimen but let the current run so as=
to clean the target.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
I have had to repair HT cables in many countries of the world where it wa= s rare for a new HT cable to be on hand. It is not the problem that you m= ay be expecting.
There are many other industries that use HT cables, main power lines are = a good example. Look in your local Yellow pages for organisations in the fields of - power cables, x-ray generators, capacitor manufacturers, hig= h voltage transformer manufacturers etc.
They will be able to use your Hitachi cable ends and simply replace and r= e seal the cable.
Best of luck
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
Our electronics workshop on campus designed two circuits which reduce the dependance of the JEOL 100CX on expensive batteries. Please let me know if you would like to receive info about the circuits, which have served our 100CX reliably for more than 5 years.
Tony
Tony Bruton Head, Centre for Electron Microscopy University of Natal, Pietermaritzburg Private Bag X01, Scottsville 3209, KwaZulu-Natal, South Africa. Tel +27 (0)331 260 5155, Home +27 (0)331 962676 Fax +27 (0)331 260 5776 email: bruton-at-emu.unp.ac.za
Depending on the current and stability requirements... You might want to check out replacing the batteries with active solid state devices. Although more stable references are available, the LM317 adjustable voltage regulator (for less than $2.00) may do the job. The output voltage is set using a two resistor voltage divider and an internal reference. Be sure to use stable resistors - wirewound or metal film.
Woody White, Electron Microscopist SEM/EDS/WDS
Work: Mcdermott Technology, Inc. woody.n.white-at-mcdermott.com http://www.mtiresearch.com/
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Hello fellow subscribers,
Early versions of the JEOL 100CX TEM use 16.2volt and 8.1volt mercury batteries as reference voltage source. There is also a 29.7volt mercury battery in the objective lens circuit.
Now that mercury batteries are no longer available, can anyone tell me if there are any suitable substitutes available, preferably in the UK?
Regards,
Bob Phillips
MicroServiS
11 Grafton Close, St. Ives, Huntingdon, http://dspace.dial.pipex.com/microservis/ Cambs., PE17 6DL
id AA17924 for Microscopy-at-Sparc5.Microscopy.Com; Fri, 5 Jun 98 05:02:25 MST Received: from msgphx3.sps.mot.com by mogate.sps.mot.com (4.1/SMI-4.1/Email-2.0) id AA16550 for Microscopy-at-Sparc5.Microscopy.Com; Fri, 5 Jun 98 05:02:24 MST Received: from email.sps.mot.com ([221.105.10.56]) by msgphx3.sps.mot.com (Netscape Messaging Server 3.01) with ESMTP id AAA29662; Fri, 5 Jun 1998 05:02:22 -0700 Message-Id: {3577DDD4.7D454685-at-email.sps.mot.com}
Bob,
I had the same problem with my cameras, the substitution that we made was a silver/air or zinc/air cell. It's about 5:00 AM here and I don't recall clearly which metal system is used. The cameras are elsewhere so I can't check. These cells have the same voltage output, my cameras work fine with this substitution. The life of these cells isn't quite as good, one opens an air seal on the battery to start the cell. Hope this somewhat fuzzy information helps!!
Bob Phillips wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello fellow subscribers, } } Early versions of the JEOL 100CX TEM use 16.2volt and 8.1volt mercury } batteries as reference voltage source. There is also a 29.7volt mercury } battery in the objective lens circuit. } } Now that mercury batteries are no longer available, can anyone tell me if } there are any suitable substitutes available, preferably in the UK? } } Regards, } } Bob Phillips } } MicroServiS } } 11 Grafton Close, } St. Ives, } Huntingdon, http://dspace.dial.pipex.com/microservis/ } Cambs., } PE17 6DL
Hello all, Since the source of gold is up, I'll toss in my 2 cents worth. Years ago we too thought of the gold coin. Looking into it we found that most gold coins were not near 99.9%. It's just too soft & of course folks wanted more than the gold value for their coins. The specialty suppliers can be quite expensive (say a foctor of 3-4). The deal of the day was to purchase "gold shot". This is the stock from which jewelers make their alloys (10kt, 18kt, etc.) The purity was claimed to be 99.99% & it cost us the current exchange rate +~$10US.
Position announcement: Member, Clinical Studies Staff
Medjet Inc., a publicly held research and development firm specializing in the design and development of ophthalmic surgical devices, is now interviewing for a position available within its Department of Clinical Studies. Preferred candidates will have completed a bachelors degree in either a biological science or biomedical engineering. Candidates will be expected to have prior training and/or experience with all phases of light, scanning, and transmission electron microscopy, photography, and animal handling. Prior experience with medical device design and manufacturing is a plus. The individual will assist in the coordination, execution, and documentation of research activities including clinical trials involving animal and human subjects.
Headquarters are located in Edison, NJ. Candidates will be considered until the position has been filled. Selected candidates will be expected to begin employment during early July and be flexible considering the potential for prolonged travel stays. Salary range is between $27,500-$32,500 per year in addition to a complete benefits package. Interested applicants should forward a resume with cover letter to the attention of Daniel Caruso.
Medjet Inc. Suite 301 1090 King George Post Road Edison, NJ 08837
As a result of my recent comments over this list server, I have received several requests for additional information about diffusion pump fluids. Here are the answers to those inquiries:
The chemical compositions of the oils I discussed previously are: Octoil-S: di(2-ethylhexyl)-sebacate DC-704: tetraphenyl-tetramethyl-trisiloxane DC-705: pentaphenyl-trimethyl-trisiloxane Santovac-5: mixture of five-ring polyphenylethers with MM = 454 Alcatel 220: eicosyl naphthalene
The common Krytox diffusion pump fluids are high molecular weight perfluorinated-alkylpolyether (perfluoroalkyl ether) fluids, manufactured by the DuPont Company, with approximately the following properties (in the same units given previously):
I would repeat, all this information, and many other goodies, can be found in my book "Vacuum Methods in Electron Microscopy" (for info about it see: http://www.2spi.com/catalog/books/book48.html and http://www.bookshop.co.uk/portland/).
I might also comment that another source has indicated that Lion-S DP oil is eicosyl naphthalene, similar to the Alcatel-200 fluid, rather than di-(2-ethylhexyl)-sebacate, like Octoil-S.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
Earlier I received many helpful comments regarding the different types of SEM's. My question now is what experiences do users of FE-SEM's have with cold cathode field emitters vs. thermal field or Schottky field emitters. Please include comments on resolution, vacuum problems and requirements, and interference from building and/or room environmental conditions. Samples will include both textiles and biologicals. Thank you in advance.
Bruce F. Ingber Bruce F. Ingber Biologist- Electron Microscopy USDA-ARS, SRRC 1100 Robert E. Lee Blvd. New Orleans, LA 70124
} Earlier I received many helpful comments regarding the different types of } SEM's. My question now is what experiences do users of FE-SEM's have with } cold cathode field emitters vs. thermal field or Schottky field emitters. } Please include comments on resolution, vacuum problems and requirements, } and interference from building and/or room environmental conditions. } Samples will include both textiles and biologicals. Thank you in advance. } } Bruce F. Ingber } Biologist- Electron Microscopy } USDA-ARS, SRRC } 1100 Robert E. Lee Blvd. } New Orleans, LA 70124 } } (504) 286-4270; fax (504) 286-4419 } bingber-at-nola.srrc.usda.gov }
Thank you for the replies on the microwave techniques course and fluorescent cell membrane marker information requests. For the people who are interested, on florescent cell membrane labeling, DiI DiO and NBD or BODIPY-labeled phospholipids were suggested. It was strongly recommended to refer to Molecular Probes catalogue, which indeed is a very good source of information. Thank you for help again, Lilith --------------------------
What is a good procedure for rotationally aligning the upper Wollaston prism on a Zeiss microscope's DIC optical system? (By "upper" is meant the one closest to the light-source. This element is a part of the condensor assembly).
Many thanks for a workable answer.
Robley Williams (robley.williams-at-vanderbilt.edu)
I am trying to use a SIS slow scan CCD (Biocam) with the Philips CM spotscan module to image viruses at low dose. I wonder if anyone has any experience with this combination of microscope, camera and spot-scanning? Could you give me advice or tips as to how to do it?
Many thanks,
Dr Timothy F. Booth Canadian Food Inspection Agency National Centre For Foreign Animal Disease Suite T2300 1015 Arlington St. Winnipeg Manitoba R3E 3M4 CANADA http://www.hc-sc.gc.ca/main/lcdc/web/bmb/fedlab_e.html#toc email tbooth-at-em.agr.ca Tel 204 789 2022 Fax 204 789 2038
A fellow called Bob was asking about innovative solutions to expensive battery replacements in the JEOL 100CX. We posted a solution to this on the server some time ago but couldn't have seen it. His mail will not respond to me -
Bob - if you are out there then get in touch with me directly and I will tell you how we tackled the same problem with great success.
Tony
Tony Bruton Head, Centre for Electron Microscopy University of Natal, Pietermaritzburg Private Bag X01, Scottsville 3209, KwaZulu-Natal, South Africa. Tel +27 (0)331 260 5155, Home +27 (0)331 962676 Fax +27 (0)331 260 5776 email: bruton-at-emu.unp.ac.za
Your message was sent a few weeks ago and it took me a while to find the = references that I wanted to send to you. You can look at the strain in = a heterostructure with convergent beam electron diffraction analysis. = However, several years ago, Hamish Frazier and company published some = work in the MSA proceedings regarding the errors of doing this with CBED = on XTEM because of surface relaxation in the sample. You should look it = up, it was about 5-6 years ago. Doug Perovic et al. also wrote two = excellent papers on this topic. In these papers, they modeled image of = the layers and included the contribution of surface relaxation effects = to the contrast in the image. I'm sure that there are other papers on = this topic, but these are what I am readily aware of and they are very = good. Here are the references:=20
"On the electron diffraction contrast of coherently strained = semiconductor layers", D. D. Perovic et al., Phil. Mag. A,, 64(1), pp. = 1-28, 1991.
"On the electron microscope contrast of doped semiconductor layers," D. = D. Perovic et al., Phil. Mag. A,, 63(4), pp. 757-784, 1991.
} microscopy-at-MSA.microscopy.com } ---------- } From: feng-at-iris.lamel.bo.cnr.it } To: microscopy-at-sparc5.microscopy.com } Subject: relaxation in TEM specimen } Date: Thursday, May 21, 1998 5:44AM } } =20 } ------------------------------------------------------------------------=
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } Dear Sir: } I am now doing some strain analysis in the herostructure ( one } called LOCOS). At the moment, I only have result based on bulk } materials. Do } you know some good way to take into account the relaxation in this kind } of } semiconductor? } } Thanks inadvance. } } Sincerely, } } Feng Wu } =20
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD}
{META content=3Dtext/html;charset=3Diso-8859-1 = http-equiv=3DContent-Type} {!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 = HTML//EN"} {META content=3D'"MSHTML 4.71.1712.3"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} Your message was sent a few weeks ago and it took me a while to = find the=20 references that I wanted to send to you. You can look at the = strain in a=20 heterostructure with convergent beam electron diffraction = analysis. =20 However, several years ago, Hamish Frazier and company published some = work in=20 the MSA proceedings regarding the errors of doing this with CBED on XTEM = because=20 of surface relaxation in the sample. You should look it up, it was about = 5-6=20 years ago. Doug Perovic et al. also wrote two excellent papers on = this=20 topic. In these papers, they modeled image of the layers and = included the=20 contribution of surface relaxation effects to the contrast in the = image. =20 I'm sure that there are other papers on this topic, but these are what I = am=20 readily aware of and they are very good. Here are the=20 references: {/DIV} {DIV} {/DIV} {DIV} "On the electron diffraction contrast of coherently strained=20 semiconductor layers", D. D. Perovic et al., {U} Phil. Mag. A, {/U} ,=20 {U} 64 {/U} (1), pp. 1-28, 1991. {/DIV} {DIV} {/DIV} {DIV} "On the electron microscope contrast of doped semiconductor=20 layers," D. D. Perovic et al., {U} Phil. Mag. A, {/U} ,=20 {U} 63 {/U} (4), pp. 757-784, 1991. {/DIV} {DIV} {/DIV} {DIV} {/DIV} {DIV} > {A=20 href=3D"mailto:microscopy-at-MSA.microscopy.com"} microscopy-at-MSA.microscopy.c= om {/A} {BR} >=20 ---------- {BR} >From: {A=20 href=3D"mailto:feng-at-iris.lamel.bo.cnr.it"} feng-at-iris.lamel.bo.cnr.it {/A} {B= R} >To:=20 {A=20 href=3D"mailto:microscopy-at-sparc5.microscopy.com"} microscopy-at-sparc5.micros= copy.com {/A} {BR} >Subject:=20 relaxation in TEM specimen {BR} >Date: Thursday, May 21, 1998=20 5:44AM {BR} > {BR} >=20 {BR} >-----------------------------------------------------------------= ------- {BR} >The=20 Microscopy ListServer -- Sponsor: The Microscopy Society of=20
Your message was sent a few weeks ago and it took me a while to find the = references that I wanted to send to you. You can look at the strain in = a heterostructure with convergent beam electron diffraction analysis. = However, several years ago, Hamish Frazier and company published some = work in the MSA proceedings regarding the errors of doing this with CBED = on XTEM because of surface relaxation in the sample. You should look it = up, it was about 5-6 years ago. Doug Perovic et al. also wrote two = excellent papers on this topic. In these papers, they modeled image of = the layers and included the contribution of surface relaxation effects = to the contrast in the image. I'm sure that there are other papers on = this topic, but these are what I am readily aware of and they are very = good. Here are the references:=20
"On the electron diffraction contrast of coherently strained = semiconductor layers", D. D. Perovic et al., Phil. Mag. A,, 64(1), pp. = 1-28, 1991.
"On the electron microscope contrast of doped semiconductor layers," D. = D. Perovic et al., Phil. Mag. A,, 63(4), pp. 757-784, 1991.
-Scott Walck
******************************************** Scott D. Walck, Ph.D. (Work Email: Walck-at-PPG.com) Karen L. Walck Adam S. Walck Brian D. Walck=20 4718 Denbigh Ct. Allison Park, PA 15101 (412) 492-8127 ********************************************=20 } microscopy-at-MSA.microscopy.com } ---------- } From: feng-at-iris.lamel.bo.cnr.it } To: microscopy-at-sparc5.microscopy.com } Subject: relaxation in TEM specimen } Date: Thursday, May 21, 1998 5:44AM } } =20 } ------------------------------------------------------------------------=
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } Dear Sir: } I am now doing some strain analysis in the herostructure ( one } called LOCOS). At the moment, I only have result based on bulk } materials. Do } you know some good way to take into account the relaxation in this kind } of } semiconductor? } } Thanks inadvance. } } Sincerely, } } Feng Wu } =20
******************************************** Scott D. Walck, Ph.D. (Work Email: Walck-at-PPG.com) Karen L. Walck Adam S. Walck Brian D. Walck 4718 Denbigh Ct. Allison Park, PA 15101 (412) 492-8127 ********************************************
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD}
{META content=3Dtext/html;charset=3Diso-8859-1 = http-equiv=3DContent-Type} {META content=3D'"MSHTML 4.71.1712.3"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {DIV} Your message was sent a few weeks ago and it took me a while to = find the=20 references that I wanted to send to you. You can look at the = strain in a=20 heterostructure with convergent beam electron diffraction = analysis. =20 However, several years ago, Hamish Frazier and company published some = work in=20 the MSA proceedings regarding the errors of doing this with CBED on XTEM = because=20 of surface relaxation in the sample. You should look it up, it was about = 5-6=20 years ago. Doug Perovic et al. also wrote two excellent papers on = this=20 topic. In these papers, they modeled image of the layers and = included the=20 contribution of surface relaxation effects to the contrast in the = image. =20 I'm sure that there are other papers on this topic, but these are what I = am=20 readily aware of and they are very good. Here are the=20 references: {/DIV} {DIV} {/DIV} {DIV} "On the electron diffraction contrast of coherently strained=20 semiconductor layers", D. D. Perovic et al., {U} Phil. Mag. A, {/U} ,=20 {U} 64 {/U} (1), pp. 1-28, 1991. {/DIV} {DIV} {/DIV} {DIV} "On the electron microscope contrast of doped semiconductor=20 layers," D. D. Perovic et al., {U} Phil. Mag. A, {/U} , = {U} 63 {/U} (4),=20 pp. 757-784, 1991. {/DIV} {DIV} {/DIV} {DIV} -Scott Walck {/DIV} {DIV} {/DIV} {DIV} {/DIV} {DIV} {/DIV} {DIV} {DIV} {/DIV} {DIV} {FONT color=3D#000000=20 size=3D2} ******************************************** {BR} Scott D. Walck, =
Ph.D. (Work Email: {A=20 href=3D"mailto:Walck-at-PPG.com"} Walck-at-PPG.com {/A} ) {BR} Karen L. = Walck {BR} Adam S.=20 Walck {BR} Brian D. Walck {/FONT} {/DIV} {DIV} {FONT color=3D#000000 size=3D2} 4718 Denbigh Ct. {BR} Allison Park, = PA =20 15101 {BR} (412)=20 492-8127 {BR} ******************************************** {/FONT} {/DI= V} {/DIV} {DIV} > {A=20 href=3D"mailto:microscopy-at-MSA.microscopy.com"} microscopy-at-MSA.microscopy.c= om {/A} {BR} >=20 ---------- {BR} >From: {A=20 href=3D"mailto:feng-at-iris.lamel.bo.cnr.it"} feng-at-iris.lamel.bo.cnr.it {/A} {B= R} >To:=20 {A=20 href=3D"mailto:microscopy-at-sparc5.microscopy.com"} microscopy-at-sparc5.micros= copy.com {/A} {BR} >Subject:=20 relaxation in TEM specimen {BR} >Date: Thursday, May 21, 1998=20 5:44AM {BR} > {BR} >=20 {BR} >-----------------------------------------------------------------= ------- {BR} >The=20 Microscopy ListServer -- Sponsor: The Microscopy Society of=20
Ph.D. (Work Email: {A=20 href=3D"mailto:Walck-at-PPG.com"} Walck-at-PPG.com {/A} ) {BR} Karen L. = Walck {BR} Adam S.=20 Walck {BR} Brian D. Walck {/FONT} {/DIV} {DIV} {FONT color=3D#000000 size=3D2} 4718 Denbigh Ct. {BR} Allison Park, = PA =20 15101 {BR} (412)=20 492-8127 {BR} ******************************************** {/FONT} {/DI= V} {/BODY} {/HTML}
Journal : Micron Volume/issue : 29/2-3, Year : 1998, ISSN : 0968-4328 Cover Date : April-June 1998
The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-White sections for large proteins S-H Brorson
Low energy loss electron microscopy of chromosomes MMG Barfels, X Jiang, YM Heng, AL Arsenault, FP Ottensmeyer pp. 105-111 Experimental model to study sedimentary kidney stones F Grases, A Llobera
Pseudo-aberration free focus condition for atomic resolution electron microscope images H Hashimoto, H Endoh, M Hashimoto, ZP Luo, MF Song
The life cycle of human immunodeficiency virus type 1 T Goto, M Nakai, K Ikuta
Transmitted color and interference fringes for tem sample preparation of silicon JP Mccaffrey
Cryo-negative staining M Adrian, J Dubochet, SD Fuller, JR Harris
Symmetry in the 2.25 MDa homomultimeric phosphoenolpyruvate synthase from Staphylothermus marinus. Analyses of negatively stained preparations G Harauz
Vesicle to micelle structural transitions involved in the interaction of dodecylbetaine with liposomes: transmission electron microscopy and light scattering studies A De La Maza, L Coderch, O Lopez, JL Parra
TEM, STM and AFM as tools to study clusters and colloids GL Hornyak, S Peschel, T Sawitowski, G Schmid
High-spatial resolution compositionally-sensitive imaging of metallic particles using plasmon energy-loss electrons in TEM EM Hunt, ZL Wang, ND Evans, JM Hampikian
Atomic force microscopy imaging of the growth features on the surface of rutile F Czerwinski, JA Szpunar
The ultrastructure of yeast: Cell wall structure and formation M Osumi
The effect of lithium treatment on collagenous tissues: an electron microscope study M Tzaphlidou, E Kounadi
Colour atlas of basic histology JR Harris
The Golgi apparatus, edited by EG Berger and J Roth JR Harris
Dear helpful readers!
I was told there are five different kinds of target available for sputtering unit and they are Gold, Gold-Palladium, Platium, Nikel and Silver
Could anyone please explain for me about the use of these targets.
What are the advantage and disadvantage on each of them? What are the effects on the quality of the image when we use the "wrong" target material? Which target material is most suitable for which kind of sample?
Any information about target material would be greatly appreciated
Thanks
Khanh Tran Khanh Tran Deakin University 662 Blackburn Road CLAYTON, VIC. 3168 AUSTRALIA
I was told there are five different kinds of target available for the sputtering unit and they are Gold, Gold-palladium, Platium, Nikel and silver
Could anyone please explain for me about the use of these targets.
What are the advantage and disadvantage on each of them? What are the effects on the image when we use the "wrong" target material? Which target is most suitable for which kind of sample?
Any information about target material would be greatly appreciated
Thanks
Khanh Tran Khanh Tran Deakin University 662 Blackburn Road CLAYTON, VIC. 3168 AUSTRALIA
there are even more. Carbon, chromium, tantalum etc.
Coatings are applied to make the sample conductive. The necessary conductivity depends on your SEM. Field-emission SEMs do not need much, so that we do not sputter most of our samples.
The disadvantage is of course that these coatings are like covers - hiding the underlying sample. You need a minimum thickness which is 2-3nm of Au-Pd or 8-12nm of chromium for most of our samples. The coating is often not smooth but shows islands. The coating smoothness depends on the sputter conditions (vacuum, sample cooling, sample rotation) and on the material. Alloys are often better than pure metals because they do not crystallize so easily. We see Au-Pd as a very good compromise (having more structure than Cr but needs much less thickness).
Khanh Tran wrote:
} I was told there are five different kinds of target available for the } sputtering unit and they are Gold, Gold-palladium, Platium, Nikel and silver } } Could anyone please explain for me about the use of these targets. } } What are the advantage and disadvantage on each of them? } What are the effects on the image when we use the "wrong" target material? } Which target is most suitable for which kind of sample?
I am interested in any information as to the use of latex microspheres for low mag. calibration. I am particularly interested in the shelf-life and stability of diluted suspensions (being able to use the diluted suspension more than one time).
First I will apologize right away to those who feel that this is off topic for the mailing list. I feel it is important to small vendors and those users who feel that it is important for vendor participation at meetings.
I known that several vendors lurk this list so I want to raise a concern about some information that has been brought to my attention. The following is included with the general information for vendors that are exhibiting at MAS/MSA '98:
"NOTE: All displays must be in the process of being installed by 6:00PM, Saturday, July 11. After that time, any unattended booths with crated displays will be set up at the discretion of M&M '98 Show Management, and all expenses will be charged to the exhibitor. Any empty booths will be reassigned by Show Management at 4:00PM Saturday, July 11 (without prior written notification). All displays must be fully installed by 11:00PM Sunday, July 12."
As a small vendor, this is totaly unacceptable and will not be tolerated. Many small vendors routinely arrive late on Saturday and setup on Sunday. To place such restrictions is not acceptable. I'm paying good money and time to support the exhibition and will not be dictated to as to when we arrive and setup. Even more so when that information arrives after we have already made travel plans. These types of stupid restrictions are exactly why vendor participation is decreasing. I realize that the Show Management would like the crates removed as soon as possible so they can install carpet and such. But to say that empty booths will be reassigned by 4:00PM on Saturday. This is going much too far.
Scott ----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
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Dear Friends,
I am currently looking at the growth/ interface studies of oxygen on silver. Does anyone know of work/ publications in this area.
regards
Parmjit
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begin: vcard fn: Parmjit S. Flora n: Flora;Parmjit S. org: University of Alberta adr: University of Alberta, Department of Physics, Faculty of Science;;412 Avadh Bhatia Physics Laboratory;Edmonton;Alberta;T6G 2J1;Canada email;internet: pflora-at-laser.phys.ualberta.ca title: Dr. tel;work: 403-492-4123 tel;fax: 403-492-0714 tel;home: 403-482-5840 x-mozilla-cpt: ;-1 x-mozilla-html: FALSE version: 2.1 end: vcard
I am interested in any information as to the use of latex microspheres for low mag. calibration. I am particularly interested in the shelf-life and stability of diluted suspensions (being able to use the diluted suspension more than one time).
I came across this article in hardcopy form while waiting for a recent dentist appointment. Its a rather longish look at ethical concerns regarding nature photography (e.g., National Geographic, and others). It seems microscopists aren't the only group grappling with where the fine line is between cleaning up an image for publication and manipulating the data.
"Photography in the Age of Falsification" by: Kenneth Brower, Atlantic Monthly, May 1998
http://www.theatlantic.com/issues/98may/photo.htm
Yours, Doug ..................................................................... : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212A) P.O. Box 245044 : : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu) : :...................................................................: http://www.pharmacy.arizona.edu/exp_path.html Home of: "Microscopy and Imaging Resources on the WWW"
Hello! I am working on a Murdock Grant this summer and am having a difficult time locating information on TEM specimen preparation for frog abdominal skin. A colleague and I are studying acid toxicity of frog epithelial tissue and need electron micrographs to observe damage done to the tight junctions due to increased pH. I have a few books on order, but am worried that they won't have the information that I need. I also need to know how to adjust my buffer systems for we are studying the ion permeability of the skin at a number of acidic pH's. I am using a glutaraldehyde/osmium tetraxide fixative and a Ringer's solution for a buffer. If you could provide me with any info, references, tips, etc. I would be very appreciative.
This is a multi-part message in MIME format. --------------EB0A8AC9A2353FBEE181809A Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit
Dear Friends,
I am currently looking at the growth/ interface studies of oxygen on silver. Does anyone know of work/ publications in this area.
regards
Parmjit
--------------EB0A8AC9A2353FBEE181809A Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Parmjit S. Flora Content-Disposition: attachment; filename="vcard.vcf"
begin: vcard fn: Parmjit S. Flora n: Flora;Parmjit S. org: University of Alberta adr: University of Alberta, Department of Physics, Faculty of Science;;412 Avadh Bhatia Physics Laboratory;Edmonton;Alberta;T6G 2J1;Canada email;internet: pflora-at-laser.phys.ualberta.ca title: Dr. tel;work: 403-492-4123 tel;fax: 403-492-0714 tel;home: 403-482-5840 x-mozilla-cpt: ;-1 x-mozilla-html: FALSE version: 2.1 end: vcard
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Parmjit S. Flora wrote:
} Dear Friends, } } I am currently looking at the growth/ interface studies of oxygen on } silver. Does anyone know of work/ publications in this area. } } regards } } Parmjit } } ------------------------------------------------------------------------ } } Parmjit S. Flora {pflora-at-laser.phys.ualberta.ca} } Dr. } University of Alberta } } Parmjit S. Flora } Dr. {pflora-at-laser.phys.ualberta.ca} } University of Alberta } University of Alberta, Department of Physics, Faculty of Science Work: 403-492-4123 } 412 Avadh Bhatia Physics Laboratory Fax: 403-492-0714 } Edmonton Home: 403-482-5840 } Alberta Netscape Conference Address } T6G 2J1 } Canada } Additional Information: } Last Name Flora } First Name Parmjit S. } Version 2.1
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Does anyone know of a way to visualize the tempering on a piece of window = safety glass? I've tried cross sectioning followed by etching with 10% = HFwith no indication of any differences through the thickness of the = glass.
The only thing I've noticed so far is a difference in the fracture = surface pattern near the edge of the glass (about 1 mm on each side). = Near the edge a ridge pattern runs perpendicular to the glass surface = while in the center the fractured surface is mostly smooth with some = ridging running roughly parallel to the surface in the middle. (Total = thickness of the glass is about 4 mm.)
Am I seeing tempering in the 1 mm of perpendicular ridges? Does anybody = have a ball park number for how thick a surface layer is affected by the = tempering?
Does anyone know of a way to visualize the tempering on a piece of window = safety glass? I've tried cross sectioning followed by etching with 10% = HFwith no indication of any differences through the thickness of the = glass.
The only thing I've noticed so far is a difference in the fracture = surface pattern near the edge of the glass (about 1 mm on each side). = Near the edge a ridge pattern runs perpendicular to the glass surface = while in the center the fractured surface is mostly smooth with some = ridging running roughly parallel to the surface in the middle. (Total = thickness of the glass is about 4 mm.)
Am I seeing tempering in the 1 mm of perpendicular ridges? Does anybody = have a ball park number for how thick a surface layer is affected by the = tempering?
Are there any advantages to using cacodylate buffers in fixatives as opposed to phosphate buffers? Are there any disadvantages in addition to the toxicity of cacodylate buffers?Specifically, I am working with human corneas. Thanks for your replies.
drose-at-wlgore.com wrote the following: =================================================== I am interested in any information as to the use of latex microspheres for low mag. calibration. I am particularly interested in the shelf-life and stability of diluted suspensions (being able to use the diluted suspension more than one time). ==================================================== You did not mention whether for SEM or TEM but I will assume it is SEM. Your question is particularly hard to answer since the surfactant systems vary so widely but generally speaking, the big concern is if there has been any kind of coagulation of the latex. If you shake up the vial, and don't "see" anything floating, then it is likely that it is OK. We have had diluted material at times years old which still seemed to be OK. We have diluted other latex and it seems to destabilize in just a few days.
But as a word of caution, the lower the magnification, the larger the latex sphere and the larger the sphere, the larger the expected standard deviation and also, large spheres will become unstable faster than will small ones.
Unless you have some really out-of-the-ordinary need, you might be better off using one of the calibrated SEM mounts made by electron beam lithography methods, etched into silicon. You can deposit small samples on that kind of sample which is the most useful in fact at "low magnifications" and you also have a form of built in calibration "yardstick". It can also be recleaned and reused.
You can see one such example on our website at URL http://www.2spi.com/catalog/standards/stndcal1.html
We are not the only supplier for this kind of product, most of the main suppliers of EM consumables offer their own versions which at least to the first approximation, will do the same thing.
Disclaimer: SPI Supplies offers both calibrated microspheres and a calibrated SEM mount, however, we do believe that for low magnification SEM work, the calibrated SEM mount is a better choice.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
1. Cacodylate stocks will keep longer, not allowing fungi to grow.=20 2. Cacodylate has aditional fixing activity of its own 3. Phosphate may originate some precipitates in some materials.
Disadvantages: Will precipitate with uranyl acetate (as well as phosphate). So, replace = the buffer before using uranyl acetate "in block" staining.
Dr. A.P. Alves de Matos mtlopes-at-fc.ul.pt
----- Mensagem original ----- De: Dr. Gernot Richter [SMTP:mdjt-clin-at-monmouth.com] Enviada em: Ter=E7a-feira, 9 de Junho de 1998 2:47 Para: Microscopy-at-sparc5.microscopy.com Assunto: Cacodylate buffers
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
Hello everyone,
Are there any advantages to using cacodylate buffers in fixatives as opposed to phosphate buffers? Are there any disadvantages in addition to the toxicity of cacodylate buffers?Specifically, I am working with human corneas. Thanks for your replies.
1. If you add calcium to your fixative/buffer, cacodylate would avoid precipitation of insoluble calcium phosphate.
2. Micro organisms do not grow in cacodylate buffer as they do in phosphate buffer. So you must prepare fresh phosphate more frequently...you may also store phosphate buffer in the freezer and thaw as needed.
On Mon, 8 Jun 1998, Dr. Gernot Richter wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello everyone, } } Are there any advantages to using cacodylate buffers in fixatives as } opposed to phosphate buffers? Are there any disadvantages in addition to } the toxicity of cacodylate buffers?Specifically, I am working with human } corneas. Thanks for your replies. } } Dan Caruso } Medjet Inc. } } }
************************************************************************** Delilah F. Wood Tel: 510-559-5653 USDA - ARS - WRRC Fax: 510-559-5777 800 Buchanan St. Email: wood-at-pw.usda.gov Albany, CA 94710 **************************************************************************
First, let me apologize to the vast majority of you, who are not affected by exhibitor procedures for Microscopy and Microanalysis '98, for bothering you with this, but Scott Davilla raised some concerns yesterday in this forum that need a response. Hopefully this will end this discussion.
Scott was correctly objecting to a specific section of the instructions to exhibitors concerning booth set-up times. This was inadvertently included in the M&M'98 instructions by our new Meeting Manager and does not apply to M&M'98. Any exhibitor concerned about this or any other aspect of M&M'98 is encouraged to contact the Meeting Manager via phone (708-361-6000), fax (708-361-6166) or e-mail (msa-at-tradeshownet.com). We are committed to resolving all issues in a satisfactory manner.
M&M'98, sponsored by MSA and MAS, is proud of its reputation as both the premier yearly Microscopy exhibition, and also as a meeting that cares deeply about the satisfaction of its exhibitors and scientific attendees. Speaking for MSA, we have involved the Sustaining Members Committee in all recent decision-making about the M&M meetings, and both MSA and MAS have members from exhibiting companies on their governing Councils.
Any member or attendee who has concerns about any aspect of the Meeting or membership (for MSA) can contact the Meeting Manager, the MSA Business Office, or, if that is not satisfactory, MSA Council directly. All of these groups can be accessed from the MSA WWW site, www.msa.microscopy.com.
Finally, one point raised by Scott requires correction and clarification. Building on the great success of M&M'96 in Minneapolis, the Cleveland meeting (M&M'97) broke all records for exhibit space for a recent M&M meeting. It now appears that M&M'98 in Atlanta will meet or exceed the Cleveland numbers - in fact, the Meeting Manager has just had to add 10 new booth spaces to the original plan. So we look forward to seeing you in Atlanta for what promises to be an exciting and enriching meeting.
Ernie Hall Secretary, MSA
_____________________________________________________________________________________________________ ________________________ Ernest L. Hall Manager, Microstructure and Microanalysis Program Characterization & Environmental Technology Laboratory GE Corporate Research and Development Building K-1, Room 2C12 PO Box 8 (1 Research Circle) Schenectady, NY 12301 (12309) Phone 518-387-6677; Fax -6972; Dialcomm 8-833-6677 Email: hallel-at-crd.ge.com
Does anyone know of a way to visualize the tempering on a piece of window = safety glass? I've tried cross sectioning followed by etching with 10% = HFwith no indication of any differences through the thickness of the = glass.
The only thing I've noticed so far is a difference in the fracture = surface pattern near the edge of the glass (about 1 mm on each side). = Near the edge a ridge pattern runs perpendicular to the glass surface = while in the center the fractured surface is mostly smooth with some = ridging running roughly parallel to the surface in the middle. (Total = thickness of the glass is about 4 mm.)
Am I seeing tempering in the 1 mm of perpendicular ridges? Does anybody = have a ball park number for how thick a surface layer is affected by the = tempering?
there is an excellent review of the "buffer in EM"-problem in G. Griffith's book "Fine structure in Immunocytochemistry", pp.57 Springer-Verlag, Berlin, 1993.
Hope that helps.
Heinz
} Hello everyone, } } Are there any advantages to using cacodylate buffers in fixatives as } opposed to phosphate buffers? Are there any disadvantages in addition to } the toxicity of cacodylate buffers?Specifically, I am working with human } corneas. Thanks for your replies. } } Dan Caruso } Medjet Inc.
*********************************************************************** Dr. Heinz Fehrenbach Institute of Pathology University Clinics "Carl Gustav Carus" Technical University of Dresden
I came across this article in hardcopy form while waiting for a recent dentist appointment. Its a rather longish look at ethical concerns regarding nature photography (e.g., National Geographic, and others). It seems microscopists aren't the only group grappling with where the fine line is between cleaning up an image for publication and manipulating the data. "Photography in the Age of Falsification" by: Kenneth Brower, Atlantic Monthly, May 1998 http://www.theatlantic.com/issues/98may/photo.htm
6/9/98 Reply:
For users of this listserv who haven't taken a speed reading course the above linked article covers the ethics of artistic license when applied to nature photography within this era of digital enhancement. I color microscans professionally and can easily understand the article's application to microscopy.
1-when does a digitally manipulated image become a fake. 2-how far do you go to satisfy the public's appetite for exciting images. 3-artistic expression - adding and subtracting from the image. 4-should a picture be labeled as digitally enhanced. 5-digital capabilities, is it progress or over the top.
Evidently when ethics are discussed, philosophical gray areas arise. I absolutely, without question, appreciate the importance of resolution and accuracy in the microscopist' work. However, I do believe colored scans provide entertaining education to the public. The public may not pick up on black and white photos and therefore it not available. Sometimes we don't know what color the microscopic specimens are and I am concerned about their representation. But, brown and gray is not appealing. I am also concerned about adding or subtracting parts of a subject in a microscan to gain a balanced composition. I have avoided the temptation. To lighten and darken really helps. It is now possible to do anything to an image; turn it inside out and spin it around in 3-D animation. I hope ethical discussions continue in order to provide the best scientific communication possible. Photographic digital enhancement is able satisfy public taste and how we use it is exciting new territory for communication, education, and art. All subjects that cannot be controlled but you can watch carefully.
Renee Recker Digital Design Communication Design for Biologists Web, Graphic, and Colored Microscans 16 West 16th St. NYC, NY 10011 212-675-1665 heyrenee-at-renorex.com http://www.renorex.com
Responding to the message of {Pine.SUN.3.96.980609052113.24561A-100000-at-aggie} from "Delilah F. Wood" {wood-at-aggie.pw.usda.gov} :
SNIP!
} 2. Micro organisms do not grow in cacodylate buffer as they do in } phosphate buffer.
SNIP!
One would certainly think so. However, I've noticed that 0.2 M caco. buffer, pH=7.0, after long storage times (I'm looking at a bottle of 100 ml mixed up two years ago) accumulates a bit of fluffy stuff at the bottom that is a mid-tone grey in color, but not a solid black. It kinda looks like fungal stuff, like the stuff that will grow in phosphate buffer after extended times, but I've not checked into it. I do not use such contaminated buffer in any preps I do.
Has anybody else noticed this, and if so got any idea what it is? Should I add 0.02% sodium azide as a preservative?
Thanks for any insight you can give,
Gib Ahlstrand, Minnesota Micoscopy Society Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
"Theory and practice are the same in theory, but different in practice."
We routinely use cacodylate buffer for glutaraldehyde and osmium because it gives a finer grain than phosphate.
Do remember that if you use uranyl acetate en bloc, you need to wash out the cacodyoate or the phosphate before adding the uranium because it is not soluble, and small precipitates will make the image very grainy. We use uranyl acetate in veronal acetate buffer; thus, we wash once in phosphate buffer after osmium, then twice (15-20 min each wash) with veronal buffer before adding the stain. Some folks use aqueus uranyl acetate.
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
} } } } } } } } accumulates a bit of fluffy stuff at the bottom that is a mid-tone } grey in color, but not a solid black. It kinda looks like fungal stuff, } like the } stuff that will grow in phosphate buffer after extended times, but I've not } checked into it. I do not use such contaminated buffer in any preps I do. } } Has anybody else noticed this, and if so got any idea what it is? Should } I add } 0.02% sodium azide as a preservative?
I have found the material to be fungal (light microscopy) in most cases. Sometimes, however, no hyphae may be encountered but simply amorphous material suggesting some chemical reaction (oxidation/reduction) taking place with the arsenate buffer.
I would hesitate to add azide as it is a potent poison and may affect ultrastructure - especially of mitochondria. Also, therte are the dangers of pouring azide down the drain (formation of explosive lead azide with plumbing solder).
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy Neckers Building, Room 146 - B Wing Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
Renee Recker asks ... } } On 6/8/98 Doug Cromey wrote: } } regarding ... } "Photography in the Age of Falsification" } by: Kenneth Brower, Atlantic Monthly, May 1998 } http://www.theatlantic.com/issues/98may/photo.htm } } 6/9/98 Reply: } } ... I color microscans professionally and can easily } understand the article's application to microscopy. } } 1-when does a digitally manipulated image become a } fake. } 2-how far do you go to satisfy the public's appetite } for exciting images. } 3-artistic expression - adding and subtracting from the } image. } 4-should a picture be labeled as digitally enhanced. } 5-digital capabilities, is it progress or over the top. } } Evidently when ethics are discussed, philosophical gray } areas arise. ...
If I were to condense the article and the points you make (... or maybe its the questions you raise ...), it would come down to the layman's perceptions of "photography" ... and the degree we are all ignorant. I think we all tend to put too much weight on photography being a "window on reality". It is true that many photographer's have given us accurate photos and have truly documented reality. On the other hand, because the digital darkroom has provided more tools, doesn't mean the ethical issues are all of a sudden more important ... after all, the "unsharp mask" was originally created in the darkroom. It has always been possible to remove complexity, if the photographer wanted the viewer to focus on a specific object. And, it has always been possible to remove specific objects and skew a population. Therefore, one point is that it is easier now, but digital tools are not the problem. If I wanted to make any point in particular it is that our faith in photography should be shaken only enough to ask questions. That having been said, it therefore follows the photographer be available for questions. It is interesting in this context how much more easily we can communicate with computer tools, but I don't necessarily mean photographers distribute their e-mail addresses with all their work (... altho that is one aspect of making yourself available ...). A more important aspect of being available for questions is simply accepting other peoples' doubts because photography is NOT reality ... that is, don't take it personally if someone questions your window on reality.
... my $0.02 :o)
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - http://darkwing.uoregon.edu/~mshaf/ Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu or mshaf-at-oregon.uoregon.edu
I am interested in purchasing a cryo-transfer stage for our JEOL 6300F SEM and as far as I know there are just two major manufacturers: Oxford and Emitech. As well as your valued comments any information regarding the interchangability of such systems with other microscopes would be appreciated.
Bugs *will* grow in cacodylate, particularly fungus. You can add a minute pinch of sodium azide to prevent unwanted growth in any buffer. We just throw in a few grains into 1 liter--like about a 1 mm pile on the end of a small spatula. If you need to measure to check your pile the first time, the azide should be about 0.002 M.
Remember this stuff is toxic to people as well as bugs, don't eat it!
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
This question might have been addressed previously, but unfortunately there doesn't seem to be a CGI-based search engine for the MSA archives and there isn't time to download them all and search with a text processor.
I'm doing physiological studies of cultured cells using an inverted microscope. The cells are plated on a coverslip which forms the bottom of a laminar flow chamber. The upper part of the chamber is plastic (polycarbonate, I believe) and the coverslip is sealed to it using a small bead of vacuum grease. At the end of my experiments I need to perform Ab identification of the cells that were measured using ion indicator dyes. I'd like to apply the antibodies (primary and secondary; for intracellular as well as extracellular antigens) in the chamber immediately after the experiment and record the Ab-labelled image for comparison with the ion indicator dye images. I don't want to use aldehydes to fix the tissue because I'm worried about residual aldehyde contamination of the chamber. I'm aware of several options, each with drawbacks:
1. Plate the cells on CELLocate coverslips from Eppendorf (or the equivalent from Bellco), then at the end of the experiment remove the coverslip from the chamber, fix as usual in aldehydes, apply Abs, etc. and relocate the same field of cells using the coverslip coordinates. The problem is that the amount of coverslips sold per minimum order is probably far more than I'd ever need, and they're rather expensive. Does anyone know of another supplier? Alternatively, I heard that Leica once made a small diamond knife mounted to a microscope objective "blank"; this could be moved into place underneath a coverslip by rotating the lens turret and then raised until it just touched the glass. The knife tip was slightly off-center, and it could be rotated by turning the collar on the barrel of the "objective" to inscribe a circle on the coverslip. Does anyone know about this device, or whether something like it is still available?
2. At the end of the experiment fix the cells in the chamber using cold methanol or acetone, apply Abs, etc. One problem I would anticipate is fairly severe shrinkage of the cells with these fixatives, probably to the point where a 1-to-1 image comparison would involve a degree of imagination on the part of a skeptical viewer. I'm not concerned with the ultrastructure of the tissue, which I know can be adversely affected by methanol or acetone. Several questions come to mind, however. I assume that using methanol or acetone would avoid problems of contaminating the plastic chamber for subsequent use with live tissue, but this might be erroneous; any comments? How might I keep the coverslip cold during the period of fixation on the microscope stage? Is it necessary to maintain a low temperature, or can the tissue come to room temperature during fixation (about 15 minutes)? Note: It would be important not to move the stage during the procedure.
Any alternative ideas for solving this problem would be greatly appreciated.
} 2. Micro organisms do not grow in cacodylate buffer as they do in } phosphate buffer.
SNIP!
One would certainly think so. However, I've noticed that 0.2 M caco. buffer, pH=7.0, after long storage times (I'm looking at a bottle of 100 ml mixed up two years ago) accumulates a bit of fluffy stuff at the bottom that is a mid-tone grey in color, but not a solid black. It kinda looks like fungal stuff, like the stuff that will grow in phosphate buffer after extended times, but I've not checked into it. I do not use such contaminated buffer in any preps I do.
Has anybody else noticed this, and if so got any idea what it is? Should I add 0.02% sodium azide as a preservative?
Thanks for any insight you can give,
Gib Ahlstrand
There is a short article published in Stain Technology, vol. 55 #3,1980, pp 191-192, that states that the fungus is Penicillium stoloniferum, a common labatory contaminant. Doug Bray
{P} } 2. Micro organisms do not grow in cacodylate buffer as they do in {BR} } phosphate buffer.
{P} SNIP!
{P} One would certainly think so. However, I've noticed that 0.2 M caco. buffer, {BR} pH=7.0, after long storage times (I'm looking at a bottle of 100 ml mixed up two {BR} years ago) accumulates a bit of fluffy stuff at the bottom that is a mid-tone {BR} grey in color, but not a solid black. It kinda looks like fungal stuff, like the {BR} stuff that will grow in phosphate buffer after extended times, but I've not {BR} checked into it. I do not use such contaminated buffer in any preps I do.
{P} Has anybody else noticed this, and if so got any idea what it is? Should I add {BR} 0.02% sodium azide as a preservative?
{P} Thanks for any insight you can give,
{P} Gib Ahlstrand {BR}
{P} There is a short article published in Stain Technology, vol. 55 #3,1980, pp 191-192, that states that the fungus is {I} Penicillium stoloniferum {/I} , a common labatory contaminant. {BR} Doug Bray {/HTML}
I just wanted to thank all the people who wrote and warned me about ozone generators. The majority recommended better ventilation or use of a HEPA and charcoal activated filter system. I'd like to share with you some of the information that I received: ***** I just read your post. Do not use an ozone generator in a room you are occupying! They generate toxic levels of ozone, which if you are already somewhat sensitive to chemicals can make you worse. They should only be used to kill mold or destroy other alergens in an unoccupied room, then turned off and the room vented well before being occupied. To run while you are in a lab or darkroom, I'd use a charcoal and HEPA filter such as an Envirocaire or Austinaire that I use in my office and bedroom respecively. Cost about $300-400 for a good one, or $3000 for a whole-lab air purification system. ***** Ozone generators are a health risk. Please refer to the following link http://www.epa.gov/iaq/pubs/ozonegen.html"} EPA- "Ozone generators that are sold as air cleaners" ***** Do not use anything that would create OZONE in a darkroom. I worked with photographic based geophysical plotting systems back in the 80's and some people placed OZONE producing equipment in the darkrooms. This OZONE would cause the silver on boards to tarnish and cause circuit falured in equipment placed within the rooms.
Try HEPA filters first and also do not have airducts blowing directly across the top of equipment. The standard difusers did not work properly and some people would place shields at the bottom of the air difusors so the air would blow toward the walls... ***** I'm doubtful about ozone; granted that you will probably not approach the levels once often found in Los Angeles smog, I would prefer to avoid introducing it intentionally. You might have a little sulfur dioxide from the fixer, and the main thing ozone would do would be to convert it to sulfur trioxide, which becomes sulfuric acid.
Effective ventilation would probably be less expensive than good HEPA filtration, and a lot more helpful. ***** Ozone generator? We have a problem with excess ozone on our building (high voltage units, fluorescent ballasts, etc) rotting everything made of rubber (pipette bulbs, tubing, latex gloves, you name it).
Best bet for sensitivity to chemicals is to have an exhaust system (like the hood over a cooking range) to completely remove the chemicals rather than trying to oxidize them. If you are alergic to powdered chemicals, the HEPA might help but fumes will not be removed without using activated charcoal. If you have a unit with both HEPA and activated charcoal, that would do it. ***** Again, thanks! I hope to see many of you at MSA! best regards, beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
Position available for a 3D Light Microscopist. This individual will oversee the day-to-day operation of a microscopy facility housed in the Laboratory of Receptor Biology and Gene Expression within the National Cancer Institute. The facility will include a confocal microscope, a deconvolution-based microscope and a third instrument yet to be decided, possibly a two-photon microscope or a high-speed low-light level imaging system. The successful candidate will become involved in collaborative research activities with users of this instrumentation, and will play a key role in future decisions to upgrade the facility. Responsibilities of the position will include the assistance and training of users, preventive maintenance of the equipment, and organization of the facility. Candidates must have an ability to work well with biologists and should possess superior organizational skills. Hands-on experience in either confocal or deconvolution microscopy or both is highly desirable. Experience with computers and image processing is also a plus. Candidates with a Ph.D. are preferred. The position is permanent civil service, GS 11/12, $39-61K annual salary. Reply to this message (mcnallyj-at-dce41.nci.nih.gov) with inquiries.
{fontfamily} {param} Times {/param} {bigger} {bigger} {bigger} POSTDOCTORAL POSITIONS IN INTERFACE PHYSICS
DEPARTMENT OF PHYSICS, UNIVERSITY OF ILLINOIS AT CHICAGO
{/bigger}
Three postdoctoral positions are available in the Interface Physics Group at the University of Illinois at Chicago (UIC). Research in the Interface Physics Group focuses on the use atomic resolution imaging and analytical techniques in electron microscopy, coupled with theoretical simulations, to determine the structure-property relationships at internal interfaces on the fundamental atomic scale.=20 Current research programs involve ceramics, high-Tc superconductors and optoelectronic/high-power semiconducting materials and devices. The experimental facilities to perform this research are comprehensive: a JEOL 2010 Field-Emission STEM/TEM featuring a 1.4=C5 probe size, "drift free" stage, high-angle annular dark-field detector (Z-contrast), Gatan Imaging Filter, and Noran EDS; a VG HB501A Field-Emission dedicated STEM with EDS, EELS and Auger spectrometers; a JEOL 3010 conventional TEM with digital imaging capabilities and EDS; a JEOL 6320 =46ield-Emission SEM with EDS and Cathodoluminescence; a JEOL JXA733 microprobe; and a Topometrix AFM/STM. In addition to the electron microscopes, specimen preparation facilities include a Gatan Duo-mill, =46ischione precision ion-mill, SouthBay plasma cleaner and Leica Ultramicrotome. The Interface Physics Group has a Silicon Graphics R10000 Power Indigo workstation with a Molecular Simulations' Cerius 2 package incorporating the CASTEP pseudopotential code. The physics department has additional workstations and access to the UIC Convex Exemplar Supercomputer and the National Center for Supercomputing Applications at UIUC. The three research positions are as follows:
(I) Atomic Resolution Analysis of Defects in Ionic Conductors
This position is a joint postdoctoral appointment with Professor Susanne Stemmer in the Department of Physics at UIC. Research performed by the successful candidate for this position will involve the investigation of grain boundaries and defect structures in ionic and mixed ionic/electronic conducting oxide ceramics. The aim of the program is to incorporate experimental results into comprehensive atomic scale models for ionic/electronic transport in these materials.=20 It is anticipated that this position will involve a significant amount of industrial collaboration.
(II) Atomic Resolution Studies of Defects in GaN
This position is a joint postdoctoral appointment with Dr Stephen Pennycook in the Solid State Division at Oak Ridge National Laboratory (ORNL). The successful candidate for this position will be based at UIC to make full use of the extensive MBE and film characterization facilities, but have access to the 300kV VG STEM at ORNL with its 1.26 =C5 probe and soon to be installed PEELS system for imaging and spectroscopy of dislocation core structures. The main aim of this work is to develop an atomic scale understanding of the nucleation mechanisms and electronic properties of dislocations in GaN and related materials. =20
(III) Grain Boundaries in High-Tc Superconductors
This position is a joint postdoctoral appointment with Dr Stephen Pennycook in the Solid State Division at Oak Ridge National Laboratory (ORNL). The successful candidate for this position will be based at ORNL to make full use of the extensive facilities for fabrication and characterization of high-Tc materials. The main aim of this research is to investigate the structure-property relationships at grain boundaries and defects in YBCO, correlating atomic resolution imaging and EELS with macroscopic transport properties. This will involve close collaboration with growth groups in the Solid State Division and focus on two areas: acceptor/donor doped grain boundaries and defects in the RABiTs materials.
Successful candidates will be recent Ph.D. graduates in physics, metallurgy, or materials science with a sound background in the relevent materials issues and an ambition to be part of a developing program pushing at the frontiers of interface physics. Please send a resume and publication list to Professor Nigel D. Browning at the address below. Prior experience in STEM or TEM is essential. =20 However, consideration will be based on the candidates overall potential for success in the field and applicants with prior experience in related fields are encouraged to apply. Positions are for one year initially, normally renewed for a second year with possibilities existing for further years. Salary is commensurate with experience.=20 UIC is an equal opportunity employer.
{fontfamily} {param} Times {/param} {bigger} {bigger} {bigger} POSTDOCTORAL POSITIONS IN INTERFACE PHYSICS
DEPARTMENT OF PHYSICS, UNIVERSITY OF ILLINOIS AT CHICAGO
{/bigger}
Three postdoctoral positions are available in the Interface Physics Group at the University of Illinois at Chicago (UIC). Research in the Interface Physics Group focuses on the use atomic resolution imaging and analytical techniques in electron microscopy, coupled with theoretical simulations, to determine the structure-property relationships at internal interfaces on the fundamental atomic scale.=20 Current research programs involve ceramics, high-Tc superconductors and optoelectronic/high-power semiconducting materials and devices. The experimental facilities to perform this research are comprehensive: a JEOL 2010 Field-Emission STEM/TEM featuring a 1.4=C5 probe size, "drift free" stage, high-angle annular dark-field detector (Z-contrast), Gatan Imaging Filter, and Noran EDS; a VG HB501A Field-Emission dedicated STEM with EDS, EELS and Auger spectrometers; a JEOL 3010 conventional TEM with digital imaging capabilities and EDS; a JEOL 6320 =46ield-Emission SEM with EDS and Cathodoluminescence; a JEOL JXA733 microprobe; and a Topometrix AFM/STM. In addition to the electron microscopes, specimen preparation facilities include a Gatan Duo-mill, =46ischione precision ion-mill, SouthBay plasma cleaner and Leica Ultramicrotome. The Interface Physics Group has a Silicon Graphics R10000 Power Indigo workstation with a Molecular Simulations' Cerius 2 package incorporating the CASTEP pseudopotential code. The physics department has additional workstations and access to the UIC Convex Exemplar Supercomputer and the National Center for Supercomputing Applications at UIUC. The three research positions are as follows:
(I) Atomic Resolution Analysis of Defects in Ionic Conductors
This position is a joint postdoctoral appointment with Professor Susanne Stemmer in the Department of Physics at UIC. Research performed by the successful candidate for this position will involve the investigation of grain boundaries and defect structures in ionic and mixed ionic/electronic conducting oxide ceramics. The aim of the program is to incorporate experimental results into comprehensive atomic scale models for ionic/electronic transport in these materials.=20 It is anticipated that this position will involve a significant amount of industrial collaboration.
(II) Atomic Resolution Studies of Defects in GaN
This position is a joint postdoctoral appointment with Dr Stephen Pennycook in the Solid State Division at Oak Ridge National Laboratory (ORNL). The successful candidate for this position will be based at UIC to make full use of the extensive MBE and film characterization facilities, but have access to the 300kV VG STEM at ORNL with its 1.26 =C5 probe and soon to be installed PEELS system for imaging and spectroscopy of dislocation core structures. The main aim of this work is to develop an atomic scale understanding of the nucleation mechanisms and electronic properties of dislocations in GaN and related materials. =20
(III) Grain Boundaries in High-Tc Superconductors
This position is a joint postdoctoral appointment with Dr Stephen Pennycook in the Solid State Division at Oak Ridge National Laboratory (ORNL). The successful candidate for this position will be based at ORNL to make full use of the extensive facilities for fabrication and characterization of high-Tc materials. The main aim of this research is to investigate the structure-property relationships at grain boundaries and defects in YBCO, correlating atomic resolution imaging and EELS with macroscopic transport properties. This will involve close collaboration with growth groups in the Solid State Division and focus on two areas: acceptor/donor doped grain boundaries and defects in the RABiTs materials.
Successful candidates will be recent Ph.D. graduates in physics, metallurgy, or materials science with a sound background in the relevent materials issues and an ambition to be part of a developing program pushing at the frontiers of interface physics. Please send a resume and publication list to Professor Nigel D. Browning at the address below. Prior experience in STEM or TEM is essential. =20 However, consideration will be based on the candidates overall potential for success in the field and applicants with prior experience in related fields are encouraged to apply. Positions are for one year initially, normally renewed for a second year with possibilities existing for further years. Salary is commensurate with experience.=20 UIC is an equal opportunity employer.
We have need for a double tilt - low background - cold stage for a Philips EM430 TEM. If anyone knows of one that someone is willing to part with, for free or otherwise, please contact me.
Thanks.
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor Mechanical, Materials & Aerospace Engineering University of Central Florida 4000 Central Florida Blvd., PO Box 162450 Orlando, FL 32816-2450 phone: 407 823-5770,fax: 407 823-0208 lag-at-ucf.edu
Can you help us? A student is growing very small colonies of cells and she wishes to view their ultrastructure. There are about 50 cells per colony and about 4 colonies per 3cm polystyrene petri dish. Our problem is that the colonies are growing between two layers of agar. The bottom layer is 0.5% agar in PBS and the top layer is 0.33% agar in PBS. The colonies break up and float away during processing and the agar just moves around. The colonies are not attached/embedded in the agar. We have tried (1)cutting around the colonies and sucking the whole lot up (agar + colony) and treating it as a pellet but the cells are impossible to find in the resin, and (2)we have tried to just gently fix the mass and process it as a whole but we end up with no cells as the colony breaks up and the cells disperse. Is there anyone out there who could offer a suggestion. Thanks
Sarah Ellis
Research Division Peter MacCallum Cancer Institute Locked Bag #1 A'Beckett Street Melbourne, Victoria 3000 Australia
Dear All, One of our users intends to do image analysis on color images, taken from histological sections, immunostained with DAB together with an other chromogen like for instance NBT or Fast Red. Since we are aware of the fact that we may encounter problems when comparing images taken at different days or even at different time points during a session (lamp-intensity and -color fluctuations, exposure time etc., etc.) we would welcom ANY comments and suggestions from people already doing this kind of analysis. ALL information on: * the camera system used * (automatic) correction for possible differences in imaging conditions * the image analysis software used * and other points which may be important is greatly appreciated. Many thanks in advance. Lauran Oomen *****------------------------**********----------------------------***** Lauran Oomen, The Netherlands Cancer Institute, Dept. Biophysics (H-0) Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands oomen-at-nki.nl, TEL +31-20-5121898, FAX +31-20-5121893 WWW page: http://www.nki.nl/nkidep/biofys/microscopy/digmiclo.htm
Paul Geroir asked: } I am interested in purchasing a cryo-transfer stage for our JEOL 6300F } SEM and as far as I know there are just two major manufacturers: Oxford } and Emitech. As well as your valued comments any information regarding } the interchangability of such systems with other microscopes would be } appreciated.
In addition to Oxford and Emitech, VG Microtech manufactures an SEM cryopreparation system as part of its "Polaron Range" of EM specimen preparation instrumentation. If any of you are interested in additional information, please e-mail me your postal address, and I will be pleased to send you current literature on this product.
One of these systems is currently resident in the applications lab at JEOL UK. I will be organizing a cryo-preparation seminar at JEOL USA later this year, and will let the group know the details once they have been firmed up.
Best regards, Steven E. Slap, Vice-President
******************************** Energy Beam Sciences, Inc. The Laboratory Microwave Company http://www.ebsciences.com ********************************
POSTDOCTORAL POSITION - RENAL CELL PHYSIOLOGY I have an opening for a Postdoctoral Fellow in my laboratory available after October 1, 1998. The research is concerned with the cellular and molecular biology of renal xenobiotic excretion. We use a variety of techniques including, isolated renal proximal tubules, renal cells in culture, fluorescent substrates and confocal microscopy to follow substrates across epithelial cells and to characterize the processes involved and the signal transduction pathways regulating transport. Candidates should have a Ph.D. or M.D. degree, less than 5 years postdoctoral experience and a background in cellular physiology, membrane transport or renal physiology. This is a non-tenure track position (NIH IRTA Fellow).
_____________________________ Dr. David S. Miller Laboratory of Pharmacology & Chemistry NIH/NIEHS Research Triangle Park, NC 27709
I have an old brass microscope, with two eyepieces and three objectives. I have had it since my childhood and my memory is that my father had used it in college/high school, in Seattle c. 1916-1924, and that it was second hand at that time. Since he was an economist, he did not use it professionally (no, he was not a microeconomist). The microscope has engraved on it's base, in large letters, "A. S. Aloe & Co, St. Louis, Mo." It also has, in a different font, "Diagnostician," which I take to be the model name of the instrument. There are no other identifying marks that I can discern on the body/base. The eyepieces and two of the objectives are stamped "A.S. Aloe," the third objective is inscribed "Gundlach Optical Co, Rochester N.Y." Because this third objective appears to be of the same form/fit/function/material/finish as the other two, my suspicion is that Aloe was a distributor and Gundlach was the manufacturer, but the evidence is weak. I would greatly appreciate any suggestions for tracing the history of this instrument, and particularly if there is any record of an A.S. Aloe & Co. in St. Louis, and a Gundlach Optical Co. in Rochester, both in the late 1800's or early in this century. Thx for your attention, and help.
Robert. F. Rowntree
Bob & Esther Rowntree 808 Murray Ave San Luis Obispo, CA 93405 (805)549-9107
[skip] } It has always been } possible to remove complexity, if the photographer wanted the viewer to } focus on a specific object. And, it has always been possible to remove } specific objects and skew a population.
Not to mention that by varying the f-stop, speed, or lighting conditions the original photograph can be chosen to highlight what the photographer wants. Yours, Bill Tivol
I routinely use an image analysis system to do exactly what you describe below. So here are the specifics:
* the camera system used For the last 6 years I've used the Sony DXC-151 CCD/RGB camera which exports the image directly into the frame grabber board in my computer. It should be noted that the system I use is compatible with just about any kind of camera system and I often work with images that were made with other camera systems (both digital and video) which are on diskettes in tiff or jpeg format.
* (automatic) correction for possible differences in imaging conditions Corrections for different conditions can be made either directly after the image is 'grabbed' or while setting the color thresholds, most of the time I've found that it's really not necessary.
* the image analysis software used BioQuant TCW by: R&M Biometrics 5611 Ohio Ave. Nashville, TN 37209 615-350-7866
They have a web site, search for bioquant.com
Cost (including computer) is under US$30,000
* and other points which may be important Make sure that the system you get is open ended enough so that other applications such as densitometry, stenography, area and line measurements etc. can also be performed. You might be surprised at how much you will want to use these additional applications. Also, it should be relatively simple to transfer the collected data to a spreadsheet. BQ does all of the preceding.
No Suggestions I am simply a very satisfied customer and have no financial or business interest in R&M Biometrics
-- Begin original message --
Dear All, One of our users intends to do image analysis on color images, taken from histological sections, immunostained with DAB together with an other chromogen like for instance NBT or Fast Red. Since we are aware of the fact that we may encounter problems when comparing images taken at different days or even at different time points during a session (lamp-intensity and -color fluctuations, exposure time etc., etc.) we would welcom ANY comments and suggestions from people already doing this kind of analysis. ALL information on: * the camera system used * (automatic) correction for possible differences in imaging conditions * the image analysis software used * and other points which may be important is greatly appreciated. Many thanks in advance. Lauran Oomen *****------------------------**********----------------------------***** -- End original message --
regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**I'm willing to make the mistakes if someone else is willing to learn from them**
Dear Sarah, } } Can you help us? A student is growing very small colonies of cells and } she wishes to view their ultrastructure. There are about 50 cells per } colony and about 4 colonies per 3cm polystyrene petri dish. Our problem } is that the colonies are growing between two layers of agar. The bottom } layer is 0.5% agar in PBS and the top layer is 0.33% agar in PBS. The } colonies break up and float away during processing and the agar just } moves around. The colonies are not attached/embedded in the agar. } We have tried (1)cutting around the colonies and sucking the whole lot } up (agar + colony) and treating it as a pellet but the cells are } impossible to find in the resin, and (2)we have tried to just gently } fix the mass and process it as a whole but we end up with no cells as } the colony breaks up and the cells disperse. } Is there anyone out there who could offer a suggestion.
I am most certainly not an expert on this, but could you try cut- ting around the colonies, cooling the dish to 0 C (to harden the agar, but not freeze the cells or medium), then extract the agar + cells? If this gives you the intact colony between the agar layers, you could then trim away some of the agar. Good luck. Yours, Bill Tivol
Gernot, Cacodylate is probably one of the most cytoplasmic extracting buffers where as pbs or phosphate is better but must be replaced before osmication. My rule is PBS or phosphate buffers for immuno-EM and cacodylate for standard fixations. Check out Hyat or Glauret's text series on fixation/buffers.
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} } Dear Sarah, } } } } Can you help us? A student is growing very small colonies of cells and } } she wishes to view their ultrastructure. There are about 50 cells per } } colony and about 4 colonies per 3cm polystyrene petri dish. Our problem } } is that the colonies are growing between two layers of agar. The bottom } } layer is 0.5% agar in PBS and the top layer is 0.33% agar in PBS. The } } colonies break up and float away during processing and the agar just } } moves around. The colonies are not attached/embedded in the agar. } } We have tried (1)cutting around the colonies and sucking the whole lot } } up (agar + colony) and treating it as a pellet but the cells are } } impossible to find in the resin, and (2)we have tried to just gently } } fix the mass and process it as a whole but we end up with no cells as } } the colony breaks up and the cells disperse. } } Is there anyone out there who could offer a suggestion. } } I am most certainly not an expert on this, but could you try cut- } ting around the colonies, cooling the dish to 0 C (to harden the agar, but } not freeze the cells or medium), then extract the agar + cells? If this } gives you the intact colony between the agar layers, you could then trim } away some of the agar. Good luck. } Yours, } Bill Tivol
Margaret Springett e-mail hukee.margaret-at-mayo.edu IEM Specialist at Mayo Foundation 1426 Guggenheim Rochester, Mn. 55905
We are looking to upgrade our Cambridge S250 SEM with a slow scan image digitiser. Specifically we are looking for a system to grab the image from the photo tube at fairly high resolution (at present we can only grab at video res from the internal digital image store which does not work for BSE images). I do not think we can afford a system which controls the beam scan itself - besides this is probably beyond our requirements.
If anybody has recently acquired such a system and has an opinion on what is best or how to go about it I would be grateful.
Please reply to me direct and I will provide the list with a summary.
Many thanks,
Stu
P.S. This morning's headline in the Independent newspaper in the UK:
"...at 4:30pm today a man will kick a ball" ---------------------- Stuart Kearns stuart.kearns-at-bris.ac.uk
Dear Friends, I was disappointed last year when Victawet, the release agent I used on my carbon evaporator, was taken off the market because the manufacturers didn't want to deal with the safety labeling issues, so I was told. I had found Victawet a very effective release agent, and I have to clean my bell jar quite frequently in our busy SEM/EDX lab. I have been using the spray-on release agents since then and I find them expensive and very ineffectual, making the bell-jar cleaning process a long, hard scrub. Last month, in frustration, I lathered up my hands with the bar soap I keep at my sink and smeared it inside my bell-jar. I figured it couldn't be worse than the spray-on. It worked beautifully! Yesterday I wiped it with a wet sponge and everything lifted off with no effort. It does not seem to affect the high vaccuum performance and you can't beat the price. Makes me wonder what I have been spending all my scarce money on all this time.
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca
To all Midwest microscopists in Central States or MIKMAS societies.
The Ballot sent out this week had an incorrect fax #.
The correct Fax # for Lou Ann is: 217-244-1652
Thanks,
Lou Ann
***************************************-at-redfoot Lou Ann Miller Center for Microscopy & Imaging College of Veterinary Medicine Dept. of Veterinary Biosciences University of Illinois Rm 1108 Basic Sciences Bld 2001 S Lincoln Ave. Urbana, Illinois 61802
We are looking to upgrade our Cambridge S250 SEM with a slow scan image digitiser. Specifically we are looking for a system to grab the image from the photo tube at fairly high resolution (at present we can only grab at video res from the internal digital image store which does not work for BSE images). I do not think we can afford a system which controls the beam scan itself - besides this is probably beyond our requirements.
If anybody has recently acquired such a system and has an opinion on what is best or how to go about it I would be grateful.
Please reply to me direct and I will provide the list with a summary.
Many thanks,
Stu
P.S. This morning's headline in the Independent newspaper in the UK:
"...at 4:30pm today a man will kick a ball"
---------------------- Stuart Kearns stuart.kearns-at-bris.ac.uk
Data Translation (http://www.datx.com) sells a slow scan board, the DT3152. It accepts just about any video signal. Setting it up for analog X-Y rasters can be a chore but the images (up to 4Kx4K I believe) are very good.
Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 (978) 750-1717 crossman-at-osi.sylvania.com
You might try first infiltrating the cells and agar in situ with a protein like bovine serum albumin (or possibly gelatin) and then fixing the entire petri dish contents by gently overlaying some 4% glutaraldehyde. The glut should not mix with the albumin but should layer on top of it. Allow this to stand for several hours. The glut will diffuse into the albumin, cross link it to a firm configuration that you will be able to cut with a razor blade into cubes. You will probably have to re-fix the blocks that you initially trim out to firm them up even more. An additional hour in glut should do this.
You should experiment with this first to get the times and proper concentrations of protein, but we used it successfuly a number of years ago. You might even use microwaving to accelerate the fixation.
A second possibility would be to do a freeze substitution fixation. But this will be more complicated. Try the easier one first.
Keep us all informed as to your results.
} Can you help us? A student is growing very small colonies of cells and } she wishes to view their ultrastructure. There are about 50 cells per } colony and about 4 colonies per 3cm polystyrene petri dish. Our problem } is that the colonies are growing between two layers of agar. The bottom } layer is 0.5% agar in PBS and the top layer is 0.33% agar in PBS. The } colonies break up and float away during processing and the agar just } moves around. The colonies are not attached/embedded in the agar. } We have tried (1)cutting around the colonies and sucking the whole lot } up (agar + colony) and treating it as a pellet but the cells are } impossible to find in the resin, and (2)we have tried to just gently } fix the mass and process it as a whole but we end up with no cells as } the colony breaks up and the cells disperse.
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy Neckers Building, Room 146 - B Wing Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
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We're considering the feasibility of purchasing a new transmission scope and are gathering info to decide initially which styles and makes of scopes would be most appropriate for our needs. We currently have a Phillips 300 which works beautifully (thanks to Mark Huller, our excellent local Phillips service tech), however, we've become aware that replacement parts are quickly becoming unavailable in the event of a major repair. Since the institute is planning construction of a new research building, it only makes sense to plan the purchase of a new scope as well.
To date, all our specimens have been biological in nature and analyses involving EELS, X-ray, diffraction, etc are not likely (but not outside the realm of possibility). Usage isn't expected to be particularly heavy (although that is also certainly subject to change). We've made the usual paper prints from our negatives but more recently have simply scanned the negs and "processed" them using Adobe Photoshop. Ideally, should the technology be adequate, we would like the capability to electronically capture the images directly from the scope as well as continue with conventional darkroom techniques.
Should anyone have any suggestions or recommendations with regards to manufacturers, models, and imaging capability and quality, I'd be grateful. Communications may be sent directly to me.
Thanks,
Jaclynn M. Lett, Research Assistant
jmlett-at-cid.wustl.edu
Central Institute for the Deaf Center for the Study of the Biology of Hearing and Deafness 818 S. Euclid Ave. St. Louis, MO 63110
Stuart Kearns wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi Folks, } } We are looking to upgrade our Cambridge S250 SEM with a } slow scan image digitiser. Specifically we are looking for } a system to grab the image from the photo tube at fairly } high resolution (at present we can only grab at video res } from the internal digital image store which does not work } for BSE images). I do not think we can afford a system } which controls the beam scan itself - besides this is } probably beyond our requirements. } } If anybody has recently acquired such a system and has an } opinion on what is best or how to go about it I would be } grateful. } } Please reply to me direct and I will provide the list with } a summary. } } Many thanks, } } Stu } } P.S. This morning's headline in the Independent newspaper } in the UK: } } "...at 4:30pm today a man will kick a ball" } ---------------------- } Stuart Kearns } stuart.kearns-at-bris.ac.uk I've been evaluting GW's "printerface". Works great for the money.
I think you have wasted your money by buying in the past special cleaning agents. I have been in the EM field for more years than I like to remember (} 35 years). I have never used any special cleaning agents and clean the bell yar of the evaporator after usage with alcohol and paper towels. I experienced no problems and the bell yar is as clean as it was when we bought the unit.
Hans Brinkies Swinburne, University of Technology School of Engineering and Science P.O.Box 218 HAWTHORN. Vic. - 3174 - Melbourne, Australia
they have a very good section on historical microscopes.
Hans Brinkies Swinburne, University of Technology School of Engineering and Science P.O.Box 218 HAWTHORN. Vic. - 3174 - Melbourne, Australia Hans G Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science Electron Microscopy Hawthorn, 3122, Melbourne - Australia
Funny that Mary. I've been applying a thin coating of detergent, well dried before use, on vacuum bell jars for about 30 years. Neither did I invent that particular wheel: The Girl Guides have used soap/detergent on the outside of saucepans to help with the clean-up for at least 60 years. Which Journal would publish such a simple gem? Thanks to the microscopy server. Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
} Dear Friends, } I was disappointed last year when Victawet, the release agent I used on my } carbon evaporator, was taken off the market because the manufacturers didn't } want to deal with the safety labeling issues, so I was told. I had found } Victawet a very effective release agent, and I have to clean my bell jar } quite frequently in our busy SEM/EDX lab. I have been using the spray-on } release agents since then and I find them expensive and very ineffectual, } making the bell-jar cleaning process a long, hard scrub. Last month, in } frustration, I lathered up my hands with the bar soap I keep at my sink and } smeared it inside my bell-jar. I figured it couldn't be worse than the } spray-on. It worked beautifully! Yesterday I wiped it with a wet sponge and } everything lifted off with no effort. It does not seem to affect the high } vaccuum performance and you can't beat the price. Makes me wonder what I } have been spending all my scarce money on all this time. } } Regards, } Mary } } Mary Mager } Electron Microscopist } Metals and Materials Engineering } University of British Columbia } 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } CANADA } tel: 604-822-5648 } fax: 604-822-3619 } e-mail: mager-at-interchange.ubc.ca } }
Mary Mager wrote: =============================================== I was disappointed last year when Victawet, the release agent I used on my carbon evaporator, was taken off the market because the manufacturers didn't want to deal with the safety labeling issues, so I was told. I had found Victawet a very effective release agent, and I have to clean my bell jar quite frequently in our busy SEM/EDX lab. I have been using the spray-on release agents since then and I find them expensive and very ineffectual, making the bell-jar cleaning process a long, hard scrub. Last month, in frustration, I lathered up my hands with the bar soap I keep at my sink and smeared it inside my bell-jar. I figured it couldn't be worse than the spray -on. It worked beautifully! Yesterday I wiped it with a wet sponge and everything lifted off with no effort. It does not seem to affect the high vacuum performance and you can't beat the price. Makes me wonder what I have been spending all my scarce money on all this time. ================================================== I can assure you that Victawet® is very much alive and well, or at least it is here at SPI where we have what we project to be a lifetime supply. And so far as I know, it is also available from our competitors, such as Ladd Research and perhaps from others. There are different "grades" and there are different ways of preparing it, so the end product itself might not be the same in appearance.
You can find Viactawet listed on our website.
This product is indeed one of those items that once you use it, it is almost impossible to imagine life with out it. And for the price of a 5 gm vial, which is not that much more in cost than that of several bars of soap, you get what would be a near life time supply for many laboratories.
Your point that there are alternatives to Victawet is well taken. But there are other applications, such as serving as a release agent for carbon films and Pt/C replicas for which so far as I know there are no alternatives.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
I need the instruction manual for the Cryo-ultra mocrotomes - LKB I and - LKB II Does anybody know where I can get them? Unfortunately, the company LKB does not exist any more, so I do not know who I can contact. Any help will be highly appriciated. Thank you very much in advance.
Manuela
---------------------- Manuela Finke University of Bristol Department of Oral and Dental Science Dental Materials and Biomaterials Group Lower Maudlin Street Bristol BS1 2LY tel:0117/9284537 fax:0117/9284780 e-mail:M.Finke-at-bris.ac.uk
We have need for a double tilt - low background - cold stage for a Philips EM430 TEM. If anyone knows of one that someone is willing to part with, for free or otherwise, please contact me.
Thanks.
************************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor Dept. of Mechanical, Materials, and Aerospace Eng.
Director, Cirent/UCF Materials Characterization Facility President, Florida Society for Microscopy
University of Central Florida phone (407) 823-5770 PO Box 162450 fax (407) 823-0208 4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu Orlando, FL 32816-2450 USA MCF (407) 275-4354 ----------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain *************************************************************************
I need the instruction manual for the Cryo-ultra mocrotomes - LKB I and - LKB II Does anybody know where I can get them? Unfortunately, the company LKB does not exist any more, so I do not know who I can contact. Any help will be highly appriciated. Thank you very much in advance.
Manuela
---------------------- Manuela Finke University of Bristol Department of Oral and Dental Science Dental Materials and Biomaterials Group Lower Maudlin Street Bristol BS1 2LY tel:0117/9284537 fax:0117/9284780 e-mail:M.Finke-at-bris.ac.uk
I assume that you mean the LKB 14800 cryokit (when you say LKB I). This was the basic grey plastic box without foam insulation and some of the cryo-tools. I have the instructions which I could photocopy and mail to you in a few days time - please let me know.
However I don't know what you mean by cryo-ultramicrotome LKB II - is this just the updated grey box or do you mean the Cryo-Nova? In any event I can't help with that.
Have you tried contacting Leica UK (tel. 01908 666 663) who took over from Cambridge Instruments who took over from Reichert.
Malcolm Haswell Electron Microscopy School of Health Sciences University of Sunderland Sunderland SR1 3SD UK tel 0191 515 2872 ----------
Dear all,
I need the instruction manual for the Cryo-ultra mocrotomes - LKB I and - LKB II Does anybody know where I can get them? Unfortunately, the company LKB does not exist any more, so I do not know who I can contact. Any help will be highly appriciated. Thank you very much in advance.
Manuela
---------------------- Manuela Finke University of Bristol Department of Oral and Dental Science Dental Materials and Biomaterials Group Lower Maudlin Street Bristol BS1 2LY tel:0117/9284537 fax:0117/9284780 e-mail:M.Finke-at-bris.ac.uk
by cap2.capitalnet.com (8.8.5/8.8.5) with SMTP id IAA07709 for {Microscopy-at-sparc5.microscopy.com} ; Thu, 11 Jun 1998 08:09:40 -0400 (EDT) Message-Id: {3.0.2.32.19980611081411.00a39584-at-capitalnet.com} X-Sender: dpurdy-at-capitalnet.com X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.2 (32)
About six months ago, I posted a message on this group indicating I was searching for a used phototube for a Wild M7A or M3Z stereomicroscope. I was also searching for an inclined binocular tube for either the M7A or M3Z.
I am still looking for these two parts. If anyone has a spare unit in good condition they would like to sell please reply to this message with a description, price and your location.
As usual in all scientific work, the integrity, education, background and reputation of the individual generating the images is the bottom line when it comes to "truth" contained within the images, or any other work one does. The characteristics aren't exclusive, a "good" scientist can still make judgmental and emotional errors, but one has to start somewhere. If the images contain information that is clear and well documented I don't see that it is terribly important to know exactly how they were generated ( except possibly from the point of view of replicating the work).
Manuela, LKB was bought out by Leitz (as was Reichert) and they renamed themselves "Leica" - not after the Russian space-dog, but the best early 35mm camera. I believe that Leica, if you find the right person, can and will help. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
} } Dear all, } } I need the instruction manual for the Cryo-ultra mocrotomes } - LKB I and } - LKB II } Does anybody know where I can get them? } Unfortunately, the company LKB does not exist any more, so } I do not know who I can contact. } Any help will be highly appriciated. Thank you very much in } advance. } } Manuela } } ---------------------- } Manuela Finke } University of Bristol } Department of Oral and Dental Science } Dental Materials and Biomaterials Group } Lower Maudlin Street } Bristol BS1 2LY } tel:0117/9284537 } fax:0117/9284780 } e-mail:M.Finke-at-bris.ac.uk } } }
Forgive my ignorance, but if the freezing point of isopentane is 113 K and that of propane is 84 K, how come a mixture of these freezes at temperatures below 77 K (boiling LN2)?
Thanks you.
James Wesley-Smith EM Unit University of Natal Durban South Africa
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An apology to all--
I recently posted a message to the listserver and it has come to my attention that I'd inadvertently turned on the "return receipt" feature of my email program (a feature which hasn't seemed to work to this point), consequently filling my mailbox with return notifications. If this has caused any congestion in the operation of the listserver, I'm truly sorry.
by hil-img-8.compuserve.com (8.8.6/8.8.6/2.12) id KAA02206 for Microscopy-at-MSA.Microscopy.Com; Thu, 11 Jun 1998 10:49:08 -0400 (EDT)
On June 11, 1998 James Wesley-Smith wrote:
"Forgive my ignorance, but if the freezing point of isopentane is 113 K a= nd
that of propane is 84 K, how come a mixture of these freezes at =
temperatures below 77 K (boiling LN2)?"
Reply:
Mixtures have lower freezing points due to "freezing point depression." =
The familiar example is mixing salt and water to give a brine solution at=
-15 deg C, used for making ice cream.
In the Handbook of Chemistry and Physics, a table shows that depressions of 50 to 100 degrees C are common in mixtures of organic molecules. So, this is likely why isopentane and propane together freeze below 77K, just= 7 degrees below propane.
[If you want to get quantitative, arm yourself with the freezing point depression constant for either of these compounds. You can then calculat= e the freezing point of the mixture. Ref. "Physical Chemistry" by WJ Moore= ]
Cheers,
Nathan Haese Walnut Creek, California Nathan_Haese-at-compuserve.com
The MSA Golf Tourney will be held on July 12 at 9:00 am at the Atlanta International Golf course. Attached is the registration form in Word 97 .doc format. Call Mark Rigler at 800-421-8451 if you need additional info.
Does anybody know of a manufacturer or supplier of magnetic field containment loops. We are having problems with our Hitachi S-800FE SEM - getting possible structurally transmitted magnetic interference from lab two floors up. Our SEM is located in a basement with the structural columns (steel reinforced concrete) directly above and to the side of the SEM. Interference manifests itself in the form of CRT screen "wobble" of set frequency especially noticable at high mag (} x3K). Equipment in other lab determined to be the source of the problem is a Microwave plasma reactor (interference occurs when heater is on) and an Electro-cyclotron resonance (ECR) instrument. Need urgent help.Cheers Dr Alan Templeton Centre for Physical Electronics and Materials SEEIE South Bank University 103 Borough Road, London SE1 0AA TEL 44 171 815 7521 /7571 FAX 44 171 815 7599 email templea-at-sbu.ac.uk
Our laboratory has recently started receiving a few skin biopsies to screen by EM for evidence of Ehlers Danlos disease. This is a new entity for us therefore, we have no guidelines in place for proper evaluation. What are the observations by LM? I have seen examples of the ultrastructural alterations of the collagen fibrils but am wondering how much of the collagen is affected, overall? Any feedback would be greatly appreciated!
Pat
Patrice Abell-Aleff Electron Microscopy Core Facility Mayo Clinic 200 1st Street SW Rochester, Minnesota 55905 e-mail: abellaleff.patrice-at-mayo.edu phone: 507-284-3148 fax: 507-284-9349
For those of you who cannot pull up the MSA registration form in the Word format, I have attached a tif file for your download. Call me if you have a problem at 800-204-6402.
} Forgive my ignorance, but if the freezing point of isopentane is 113 K and } that of propane is 84 K, how come a mixture of these freezes at } temperatures below 77 K (boiling LN2)? } Granted that dissolving a solid like salt in a solution leads to freezing point depression. However, I think the freezing point depression in this case might be better compared to a solution of two miscible liquids. I am used to the phenomenon being discussed with application to metals. A solution of tin and lead is liquid below the freezing points of the individual metals. With a "eutectic" mixture you encounter the maximum freezing point depression. I suppose the same principle applies here, even though the compounds are normally liquids (or even gases) at 'normal' conditions.
Spicer Consulting 10 Wentworth Drive Bedford MK41 8BB UK
Phone (+44)/(0) 234-346419
It was actually sold at the time by Agar in the UK and by Ted Pella in the US.
This was something like 6 years ago, and I cannot vouch for how up-to-date this information is. I can tell you though, that it does work!
Tony Garratt-Reed
At 04:24 PM 6/11/98 +0000, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Anthony J. Garratt-Reed MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 United States of America
Hi there, Just a quick note to let everyone know that they should send in their registrations as soon as possible for hotel rooms in Atlanta. For those of you who practice "brinkmanship" and leave everything to the last minute, I would like to warn you that there is an NAACP conference in the city from the 7th to the 19th of July and so the hotel rooms downtown are hard to come by and also there is a fairly large convention at the Merchandize Mart too. I didnt get my hotel resrvations in to the convention bureau and so had to make my own reservations and some of the hotels are saying they are fully booked. This of course doesnt mean that the convention center doesnt have a large block of rooms held for us, but it does mean that you should make reservations asap. If you have troubles the following hotels are near the Georgia World Congress Center:
Atlanta Hilton and Towers The Ritz Carlton Atlanta The Howard Johson Suites The Confort Inn Downtown The Best Western American Hotel
or you can check:
http://www.acvb.com/ac_stay.asp
Just a reminder.....
John F. Mansfield
Note new Area Code (734)
John Mansfield North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 936-3352 Cellular Phone: (734) 715-2510 (Leaving a phone message at 936-3352 is preferable to 715-2510) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
Bob Lawrence wrote: } I don't see that it is terribly important to know } exactly how they (images) were generated
This may be true in general. However, there is one type of image that should only be published with full information about exactly how it was generated, and that is a simulated HRTEM image. Each such image should have associated with it all the parameter values used to generate it as well as the name and version number of the software used. I am surprised at the number of papers I am called on to referee that do not contain such information. The parameter values should be given to inform the reader of what conditions would be necessary to obtain such an image. The software should be identified in order to allow for any peculiarities of particular software, or even errors that may be revealed later. An early version of SHRLI (Simulated High-Resolution Lattice Images) inverted beam indices in all of its diffraction patterns (SHRLI80), and a version of EMS imposed incident beam convergence at twice the input value (see 51st Proc MSA (1993) 974-975).
-Mike O'Keefe
-------------------------------------------------------------- Bob Lawrence wrote:
} Gentlefolk, } } As usual in all scientific work, the integrity, education, } background and reputation of the individual generating the images is the } bottom line when it comes to "truth" contained within the images, or any } other work one does. The characteristics aren't exclusive, a "good" } scientist can still make judgmental and emotional errors, but one has to } start somewhere. If the images contain information that is clear and } well documented I don't see that it is terribly important to know } exactly how they were generated ( except possibly from the point of view } of replicating the work).
TO: Nancy Zjaba National Semiconductor South Portland, ME
Soft Imaging System GmbH has image analysis software called analySIS. We also have specific modules for grain analysis. If you like more information, please visit our web site http://www.soft-imaging.de or contact us at sales-at-soft-imaging.com or myself and we will be glad to talk to you and send you brochures.
Kuni Tatsumoto kt-at-soft-imaging.com (888) FIND-SIS
Dr. Templeton: Below is the Web site for Spicer Consulting. They make an excellent mag field canceling system. I've used 3 of their units, the most recent purchased just 3 weeks ago.
http://www.spiceruk.co.uk/
Good luck! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-598-1291 KFAB PFA Lab Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Alan Templeton wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Does anybody know of a manufacturer or supplier of magnetic field } containment loops. We are having problems with our Hitachi S-800FE } SEM - getting possible structurally transmitted magnetic interference } from lab two floors up. Our SEM is located in a basement with the } structural columns (steel reinforced concrete) directly above and to the } side of the SEM. Interference manifests itself in the form of CRT } screen "wobble" of set frequency especially noticable at high mag } (} x3K). Equipment in other lab determined to be the source of the } problem is a Microwave plasma reactor (interference occurs when } heater is on) and an Electro-cyclotron resonance (ECR) instrument. } Need urgent help.Cheers } Dr Alan Templeton } Centre for Physical Electronics and Materials } SEEIE } South Bank University } 103 Borough Road, London } SE1 0AA } TEL 44 171 815 7521 /7571 } FAX 44 171 815 7599 } email templea-at-sbu.ac.uk
On Thu, 11 Jun 1998, Alan Templeton wrote: Hi Alan,
We have have been very interested in this problem as well. We have had trials of, and heard other good reports of, the field cancelling system sold by Oxford Instruments. Contact them at: Oxford Instruments, Research Instruments, Tubney Wood, Abingdon, Oxon. OX13 5QX. 01865-393200. Ask for Judith Brock. e-mail:- judith.brock-at-oxinst.co.uk
Unfortunately I have no connections or financial interests.
Good luck, Ron } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Does anybody know of a manufacturer or supplier of magnetic field } containment loops. We are having problems with our Hitachi S-800FE } SEM - getting possible structurally transmitted magnetic interference } from lab two floors up. Our SEM is located in a basement with the } structural columns (steel reinforced concrete) directly above and to the } side of the SEM. Interference manifests itself in the form of CRT } screen "wobble" of set frequency especially noticable at high mag } (} x3K). Equipment in other lab determined to be the source of the } problem is a Microwave plasma reactor (interference occurs when } heater is on) and an Electro-cyclotron resonance (ECR) instrument. } Need urgent help.Cheers } Dr Alan Templeton } Centre for Physical Electronics and Materials } SEEIE } South Bank University } 103 Borough Road, London } SE1 0AA } TEL 44 171 815 7521 /7571 } FAX 44 171 815 7599 } email templea-at-sbu.ac.uk }
=========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
Dear Microscopists, Can anybody tell me where to get urease antibodies? I don't know the origin of the urease (bacterial or fungal), is there an antibody recognizing both? It is for fluorescence immunolokalisation, many thanks, Arthur
PS: anybody knows interesting papers about fungal urease, role of urea etc? I didn't find very much.
Dr. Arthur Schuessler TU Darmstadt FB10 Botanik, AG Kluge Schnittspahnstr. 10 64287 Darmstadt
Integrated Dynamics Engineering 84 Cummings park Woburn, MA 01801 tel: 617-938-5120 fax: 617 938 5122 100125.1130-at-compuserve.com
} Steve Rozeveld } } Dow Chemical Co. } 1897 Bld. Door E-43 } Midland, MI 48667 } sjrozeveld-at-dow.com } % (517) 636-5167 } Fax: (517) 638-6443
} ---------- } From: Ron Doole[SMTP:ron.doole-at-materials.oxford.ac.uk] } Sent: Friday, June 12, 1998 2:08 AM } To: Microscopy Community } Subject: Re: SEM - problems with magnetic interference } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-Just my guess as to what happens. We constanly have problems trying to adjust different Monitors, software, and operating systems to match the same colors when printing out color images. We now do this subjectively, because the color balance seems to be set differently for each system. A color Standard "slide" would be a terrific start, and would at least allow the same starting point and settings to be made on hardware. Although I would expect CRT color-monitor variations over time, I expect the software will interpret color balance with precision each time, until a new version or a different process algorythm is employed. I would be greatful to try this slide. We actually have used the Television production color screen to pre-set our color monitors and synchronize them with a video camera. I am not sure how much the video lens alters the colors. You can see such a color screen on the television networks in early AM. However, even those colors screens fade due to the ambient lighting causing more need for color correction over time. So many factors come into play, resolution, screen display, printer interpretation, digital acquisition, balance, intensity, reflectivity, absorbance, transmittance, black level, that it will be almost impossible to get precise color balance, but it will be much closer than not having one. Tom Baginski Technical Coordinator for Microscopy
In response to: -a good standard available, mounted on a traditional 1"x3" slide, at reasonable price ($35-50), would a "color test plate"
Thanks for the names and addresses of companies selling field containment systems- have got a few now to be getting on with but if you know of any others please let me know - should prove very useful in solving this problem.
Cheers alan templeton. ------------------ Dr Alan Templeton Centre for Physical Electronics and Materials SEEIE South Bank University 103 Borough Road, London SE1 0AA TEL 44 171 815 7521 /7571 FAX 44 171 815 7599 email templea-at-sbu.ac.uk
There are a number of standards to look at, and it is possible to order all of them from the various standards committees. One of the most common is the IT8 target, which you can see at http://www.imagequality.com/colmgt.html.
However, there are many calibration charts around for different imaging systems, and the IT8 is not the only one that might be appropriate.
One good place to start looking is the Colour Technology Forum web page at
Another place to look is the Colour Science and Technology Web Page at http://ziggy.derby.ac.uk/web/colour.html.
billo
On Thu, 11 Jun 1998, Barbara Foster wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } .....Which brings us to an interesting question regarding color } rendition.... } } } If there were a good standard available, mounted on a traditional 1"x3" } slide, at } } reasonable price ($35-50), would a "color test plate" be of interest to } most people } } who did color video/digital photography? } } } Barbara Foster } } Consortium President } } {bold} {italic} {color} {param} 0000,8080,0000 {/param} Microscopy/Microscopy } Education } } {/color} {/italic} {/bold} 125 Paridon Street Suite 102 } } Springfield, MA 01118 } } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } } Visit our web site: { {http://www.MME-Microscopy.com/education} } } ****************************************************** } } {bold} {italic} {bigger} {bigger} MME: {/bigger} {/bigger} {/italic} {/bold} } America's first national consortium dedicated to } } customized on-site training in all areas of } } microscopy, sample preparation, and image analysis. } } {color} {param} 0000,8080,0000 {/param} Our goal: immediate growth in your productivity! } } {/color} } } } } } } At 05:10 AM 6/11/98 -0700, Bob Lawrence wrote: } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Gentlefolk, } } } } } } As usual in all scientific work, the integrity, education, } } } background and reputation of the individual generating the images is the } } } bottom line when it comes to "truth" contained within the images, or any } } } other work one does. The characteristics aren't exclusive, a "good" } } } scientist can still make judgmental and emotional errors, but one has to } } } start somewhere. If the images contain information that is clear and } } } well documented I don't see that it is terribly important to know } } } exactly how they were generated ( except possibly from the point of view } } } of replicating the work). } } } } } } } } } } } } } } } } }
In my previous note about the IT8.7 target, I didn't tell you where you could get one. Sorry. They are sold by most folk who manufacture scanners, etc. I know that Kodak and Agfa sell them.
billo
On Thu, 11 Jun 1998, Barbara Foster wrote:
} } If there were a good standard available, mounted on a traditional 1"x3" } slide, at } } reasonable price ($35-50), would a "color test plate" be of interest to } most people } } who did color video/digital photography? } }
Any idea is good one at this time. However, I forsee two issues that prevents standards to be set, the example here being in making "color test plates"as a slide reference to a digital colorist. 1) control of setting the color microscan standards: the contol of what is sellable does not belong with the microscopist or colorist. In my case the control belongs to the selling agent. That person serves the public buying interest. This may be contrary or compatable to "scientific truth". 2) every scan is different: I have colored insects and bacterias, etc. differently for each scan in order to make a greater selling pool. Accentuating the perspective of the subject can change scan to scan. Renee
........................................................................................ Renee Recker Digital Design Communication Design for Biologists Web, Graphic, and Colored Microscans 16 West 16th St. NYC, NY 10011 212-675-1665 heyrenee-at-renorex.com http://www.renorex.com
Barbara Foster wrote:
} ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------. } .....Which brings us to an interesting question regarding color rendition.... } } If there were a good standard available, mounted on a traditional 1"x3" slide, at } reasonable price ($35-50), would a "color test plate" be of interest to most people } who did color video/digital photography? } } Barbara Foster } Consortium President } Microscopy/Microscopy Education } 125 Paridon Street Suite 102 } Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site: {http://www.MME-Microscopy.com/education} } ****************************************************** } MME: America's first national consortium dedicated to } customized on-site training in all areas of } microscopy, sample preparation, and image analysis. } Our goal: immediate growth in your productivity! } } At 05:10 AM 6/11/98 -0700, Bob Lawrence wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } Gentlefolk, } } } } As usual in all scientific work, the integrity, education, } } background and reputation of the individual generating the images is the } } bottom line when it comes to "truth" contained within the images, or any } } other work one does. The characteristics aren't exclusive, a "good" } } scientist can still make judgmental and emotional errors, but one has to } } start somewhere. If the images contain information that is clear and } } well documented I don't see that it is terribly important to know } } exactly how they were generated ( except possibly from the point of view } } of replicating the work). } } } } } } } } } }
This is a good analogy, but not really a direct comparison. The mechanisms behind the freezing point depression are different between cooling eutectic alloys and cooling soluble chemical mixtures. In eutectic alloys cooling is controlled by the pooling of pure element regions within the mixture. The smallest pools eg. largest amount of pure metal pooling occurs at the eutectic composition. Then to ask why that condition causes the lowest "freezing" point is another question altogether (best answered by people with big big materials science degrees). In soluable chemical mixtures I don't believe you see any such pooling of the respective organics, so the mechanism for that is different. You must think about each percentage mixture as a bulk chemical. I am sure that there are many organic mixtures that have cooling point spikes as well which is not the case for eutectic alloys. Correct me if I'm wrong.
At 03:26 PM 6/11/98 +-200, you wrote:
} Forgive my ignorance, but if the freezing point of isopentane is 113 K and } that of propane is 84 K, how come a mixture of these freezes at } temperatures below 77 K (boiling LN2)? } Granted that dissolving a solid like salt in a solution leads to freezing point depression. However, I think the freezing point depression in this case might be better compared to a solution of two miscible liquids. I am used to the phenomenon being discussed with application to metals. A solution of tin and lead is liquid below the freezing points of the individual metals. With a "eutectic" mixture you encounter the maximum freezing point depression. I suppose the same principle applies here, even though the compounds are normally liquids (or even gases) at 'normal' conditions.
Dear colleagues, I have a Leaf MicroLumina Scanning Camera which we run on a Windows NT 4.0 System. The camera runs as a Twain plug in device under Adobe Photoshop 4.0. Here is the problem. We start the system up, capture the first image, and then save it to disk. We then go to collect a second image by reselecting the import and selecting the twain device ( MicroLumina), and here there is no response. The program has frozen. One then has to go to the NT Task manager, where you see that Adobe is not responding, and have the system end the Task. Upon restarting the Adobe program a second time, the Lumina runs fine, and no longer it or Photoshop does not hang up. Scitex Corporation is not able to give me any answers to this problem, and has no idea what is causing it. I have specifically asked if this camera was tested on NT 4.0 and With Adobe Photoshop 4.0 and was told that other systems are out there with no problems, but was not given a direct answer to my question. The format we save the file in does not appear to have anything to do with this. The Photoshop program has become hung up, as one cannot even open any other image file in PhotoShop. Has anyone experienced this with their MicroLumina on a Windows NT 4.0 system. It would not surprise me if this is related to Adobe 4.0, as version 4.0 has had some significant changes, with many not for the better. Hope to hear some good news.
Regards,
Joseph Goodhouse Confocal / EM Core Lab Manager Department of Molecular Biology Princeton University jgoodhose-at-molbio.princeton.edu 609-258-5432
Info and Images at http://www.molbio.princeton.edu/confocal/CF-EM-HOME.html
ok, ok, OK, ALREADY. Sorry about the tif posting and any trouble it caused. If you are not going to play or don't need the info- don't look at the file.
Also, in reference to the $75 fee on the recent form, some of the people who have replied have already registered at the cost of $65. That amount ($65) willl be honored and anyone who has already registered at the higher fee will receive a $10 refund.
The story behind the change had to do with the club we were going to play at originally. That club's membership voted to prohibit outside tournament play - - AFTER we had already given the info to MSA for their printing. So, we were stuck with having to find another club on short notice and thus have higher expenses because of it. In any event, we will simply bear the costs and work with the current club.
Call if you have any additional needs or questions.
Thanks, Mark W. Rigler, Ph.D., MAS Inc. 800-421-8451
Hi, does anyone knows where I can get confocal operation training? or is there any workshop for confocal operation? Your help and infomation will be greatly appreciated. Thank you.
Does anyone have experience looking at proliferating cells labelled with BrdU at the EM level? What resin is best, and is DAB/OsO4 localization adequate or are gold/silver probes necessary? Thanks, in advance, for any assistance.
Ronnie Houston Cytochemistry & Molecular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219
TO: Nancy Zjaba National Semiconductor South Portland, ME
Soft Imaging System has image analysis software called analySIS. We also have specific modules for grain analysis. If you like more information, please visit our web site http://www.soft-imaging.de or contact us at sales-at-soft-imaging.com or myself and we will be glad to talk to you and send you brochures.
Kuni Tatsumoto kt-at-soft-imaging.com (888) FIND-SIS
This is a good analogy, but not really a direct comparison. The mechanisms behind the freezing point depression are different between cooling eutectic alloys and cooling soluble chemical mixtures. In eutectic alloys cooling is controlled by the pooling of pure element regions within the mixture. The smallest pools eg. largest amount of pure metal pooling occurs at the eutectic composition. Then to ask why that condition causes the lowest "freezing" point is another question altogether. Apparently the heat capacity of that composition is greatest for the given system.
In soluable chemical mixtures I don't believe you see any such pooling of the respective organics, so the mechanism for that is different. You must think about each percentage mixture as a bulk chemical. Of course you get a cooling curve because the heat capacity of neighboring mixtures in a chemical system vary in small amounts at a time. I am sure that there are many organic mixtures that have cooling point spikes as well which is not the case for eutectic alloys. Correct me if I'm wrong.
At 03:26 PM 6/11/98 +-200, you wrote:
} Forgive my ignorance, but if the freezing point of isopentane is 113 K and } that of propane is 84 K, how come a mixture of these freezes at } temperatures below 77 K (boiling LN2)? } Granted that dissolving a solid like salt in a solution leads to freezing point depression. However, I think the freezing point depression in this case might be better compared to a solution of two miscible liquids. I am used to the phenomenon being discussed with application to metals. A solution of tin and lead is liquid below the freezing points of the individual metals. With a "eutectic" mixture you encounter the maximum freezing point depression. I suppose the same principle applies here, even though the compounds are normally liquids (or even gases) at 'normal' conditions.
After a long use of the gold coater, the gold target is thinner and thinner and finally there is a hole showing up. I believe it is the time to replace the gold target.
My question is whether I can temporally cover the hole with a small piece of gold plate so I can still use the existing gold target, before I replace it with a new one. Any suggestions about how to connect two gold plates together (glue them with carbon paste ?). Thanks.
If you are interested in specific video test images, you might look at the Hans Irtel's site at the University of Mannheim, particularly the color CRT page and the Video Test Image page.
Go to http://www.uni-mannheim.de/fakul/psycho/irtel/cvd.html
and take the link to Video Test Images.
You might also look at the CIE site, which deals with standards quite a bit.
Hope this helps.
billo
On Fri, 12 Jun 1998, Thomas A Baginski wrote:
} differently for each system. A color Standard "slide" would be a } terrific start, and would at least allow the same starting point and } settings to be made on hardware.....
We are looking for a TEM tissue processor similar to the histological instruments that are available but one that would allow the automated (or nearly so ) fixation, dehydration, embedding and polymerization into various resins. I believe BAL-TEC used to make a freeze substitution unit that appeared to allow a number of resins and protocols, the FSU 010 but I can't find any sources of information regarding this or similar instruments. Any leads or comments would be appreciated.
I wanted to second John Mansfield's suggestion that those of you who are planning to attend Microscopy and Microanalysis '98 and have not yet made hotel reservation do so as soon as possible. As of our most recent information the Marriott and the Westin still have rooms available in the M&M'98 block. You will need to contact the hotels directly at:
In some cases, we have been told that the hotels report being sold out, although there are M&M rooms available. You may want to check with the M&M contact people:
As a last resort, you can call the M&M Meeting Manager (Bud, Mary Beth, or AnnaMarie) at 708-361-6000 for help.
ALSO PLEASE REMEMBER THAT MONDAY, JUNE 15 IS THE FINAL DEADLINE FOR EARLY REGISTRATION. Registration forms are available on the MSA website or from the Meeting Manager. They need to be faxed to 708-361-6166.
I am not a microscopist, but hope that someone on the list might share some insights regarding sample preparation for wood composite materials. I am trying to prepare thin sections for optical microscopy of waferboard type composites. My need is to obtain sections perpendicular to the wood flake direction. So far, I end up with sawdust. I have tried to impregnate with spur and ultra spur epoxies - to no avail. The costraint I am bound by is that I cannot introduce any moisture or solvent into the matrix which may redistribute its components (adhesive, wax, additives). The typical composite has between 3% and 7% moisture content, an equal amount of isocyanate adhesive, and less than 1% each of wax and other additives. These additives can be water and/or organic solvent soluble.
All suggestions are welcome. I am currently at a loss as to how to proceed.
Regards,
Glenn M. Larkin Graduate Assistant Institute of Wood Research Michigan Technological University Houghton, MI 49931-1295 USA (906) 487-3316
} Does anyone have experience looking at proliferating cells labelled with } BrdU at the EM level? What resin is best, and is DAB/OsO4 localization } adequate or are gold/silver probes necessary? } Thanks, in advance, for any assistance. } } Ronnie Houston } Cytochemistry & Molecular Pathology } Texas Scottish Rite Hospital for Children } 2222 Welborn Street } Dallas, TX 75219
Try Histochemistry 95:491-494, 1991.
Geoff -- *************************************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane Piscataway, NJ 08854 voice: (732)-235-4583; fax -4029 e-mail: mcauliff-at-umdnj.edu ***************************************************************
Can someone please help Matija with this question?
} Date: Fri, 12 Jun 1998 11:37:17 -0400 (EDT) } From: Matija Maretic {matija-at-gravity.phys.utk.edu} } To: paul.perkes-at-asu.edu } MIME-version: 1.0 } } Dear Mr. Perkes, } } My name is Matija Maretic and I am doing research at University of } Tennessee at Knoxville. I know that Kodak carries a number of } scientific glass plates which can produce high quality positive image, but } I am wondering if they carry a plate which can produce a high quality } black & white negative. } Do you know where I can find this kind of information? } } Thank you, Matija Maretic }
Thanks,
Paul R. Perkes (602) 965-5218 Senior Applications Systems Analyst (602) 965-9004 FAX Center for Solid State Science (602) 952-9583 Wireless Arizona State University paul.perkes-at-asu.edu Box 871704 Tempe, AZ 85287-1704
I suggest you go to the MSA national metting this camming July 12-16. There are a lot of vendors which will be very helpful answering all your questions.
where can an individual purchase stains and chemicals for use under the microscope. Biological supply houses will not sell to me since i am not a company or school.
Thx for info. What is good source for narrowing down its decade of origin?
I have a tremor -- a shaky hand -- so I don't mouse so I don't do WINDOWS or any kind of graphics; sorry. Send me a snailmail address & I'll send you a photo.
Bob & Esther Rowntree 808 Murray Ave San Luis Obispo, CA 93405 (805)549-9107
On Wed, 10 Jun 1998 COURYHOUSE-at-aol.com wrote:
} Gundlach is the mfr! } Good scope! } Wish it was in our collection! } } Take a photo, scan it and share it with all of us! } } } Ed sharpe, archivist smecc }
} Date: Fri, 12 Jun 1998 12:53:05 -0700 } From: Paul R. Perkes {paul.perkes-at-asu.edu} } To: csssano-at-asu.edu } Cc: Matija Maretic {matija-at-gravity.phys.utk.edu} , } Microscopy-at-sparc5.microscopy.com } Subject: Kodak Plate Question } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Can someone please help Matija with this question? } } } Date: Fri, 12 Jun 1998 11:37:17 -0400 (EDT) } } From: Matija Maretic {matija-at-gravity.phys.utk.edu} } } To: paul.perkes-at-asu.edu } } MIME-version: 1.0 } } } } Dear Mr. Perkes, } } } } My name is Matija Maretic and I am doing research at University of } } Tennessee at Knoxville. I know that Kodak carries a number of } } scientific glass plates which can produce high quality positive image, but } } I am wondering if they carry a plate which can produce a high quality } } black & white negative. } } Do you know where I can find this kind of information? } } } } Thank you, Matija Maretic } } } } Thanks, } } } Paul R. Perkes (602) 965-5218 } Senior Applications Systems Analyst (602) 965-9004 FAX } Center for Solid State Science (602) 952-9583 Wireless } Arizona State University paul.perkes-at-asu.edu } Box 871704 } Tempe, AZ 85287-1704 } } Why don't you call Kodak. They have excellent tech reps. The number on their chemical catelog is 800 225-5352; you might have to get another number from them for the film division.
If this doesn't get you what you want, write me with specific info on exactly how you want to use these. There are ways to make reversal images.
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
We have moved our lab (groan) and the new site is not ideal for our scanning microscope. It looks like we need a vibration isolation table on the column section. I'm looking for recommendations for good vendors.
Many of the facility's users who do negative staining with uranyl acetate end up with an artifact that is most easily described as bubbles. These round areas of no stain are not rimmed and do not appear to be any kind of stuff, but merely absence of stain. Unfortunately, when one is looking for small virus particles and the background is covered with these un-spots, it's horribly distracting.
Can anyone shed some light on this artifact? Before I tear my hair out?
Thanks in advance,
Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I remember reading somewhere that we should not wrap contianers of uranyl acetate in aluminum foil. Why is that? Is there a danger of bremstrunghumuhumunukunukuapuaa radiation? (Sorry, it's Friday afternoon and I clearly don't know what I'm talking about.)
Aloha, Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
In response to: where can an individual purchase stains and chemicals for use under the microscope. Biological supply houses will not sell to me since I am not a company or school.
John
There are local science stores that have limited its of slides and chemicals for use with " toy" type microscopes. I would imagine the chemicals would work fine. though.
In Phx. we have one called the 'imaginarium' that sells such things.
Ask Kodak by email (they may be on the web, Kodak have a large excellent site) for the film's processing instructions. I expect that they provide for a reversal procedure (develop, expose, bleach, re-develop, fix). I expect that by using a normal procedure (develop, rinse, fix, wash, dry) these plates will behave as a normal negative film. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
} } } } My name is Matija Maretic and I am doing research at University of } } Tennessee at Knoxville. I know that Kodak carries a number of } } scientific glass plates which can produce high quality positive image, but } } I am wondering if they carry a plate which can produce a high quality } } black & white negative. } } Do you know where I can find this kind of information? } } } } Thank you, Matija Maretic } } } } Thanks, } } } Paul R. Perkes (602) 965-5218 } Senior Applications Systems Analyst (602) 965-9004 FAX } Center for Solid State Science (602) 952-9583 Wireless } Arizona State University paul.perkes-at-asu.edu } Box 871704 } Tempe, AZ 85287-1704 } } }
Dear friend, Andrei Chuvilin asked me to send you some references in O/Ag systems. I transfer to you a block of references of one of the last my papers in Catal.Letters. 47(1997)111. What is your interest in this field? because oxygen/silver system is very complicated system. With best wishes, Dr. Andrei Boronin
} In response to: } where can an individual purchase stains and chemicals for use under the } microscope. Biological supply houses will not sell to me since I am not a } company or school. } You're too pessimistic. Companies such as Carolina Biological Supply will sell to anyone. Call 800-334-5551; there are lots of stains listed in their "Science & Math" catalog
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/
The Laboratory of Neurobiology, NINDS, NIH (Bethesda, MD) has an opening for an experienced electron microscopy technician. Applicants should be knowledgable concerning the theory, principles, practices and techniques of biological electron microscopy, including operation of transmission EMs and methods for specimen preparation (fixation, embedding, staining, vacuum evaporation, immunolabeling). Competence in cryo-preparation techniques (rapid freezing, cryo-ultramicrotomy, freeze-fracture, freeze-substitution) is especially desirable. Experience with specialized fields of elemental mapping and analysis (x-ray microanalysis, electron energy loss spectroscopy, STEM, and energy-filtering TEM) would be an additional advantage. The NIH offers competitive salary and benefits packages; US citizenship required. For more information contact Brian Andrews at "sba-at-helix.nih.gov".
The phenomenon that you describe has been called champaigning. It is thought to be caused by a number of factors (none of which have been tested and proven to be the cause, however). This includes: hydrophobicity of the substrate - usually Formvar/carbon, improper spreading of the suspension, inadequate drying of the specimen on the grid, inclusion of substances - like sucrose or salts - that boil in the beam, actual particles such as ribosomes or glycogen or viral capsomeres.
Here are some things to try: - a different stain (one of the tungstates) to rule out "real" particles versus bubbles, - glow-discharge treating the grids to eliminate hydrophobicity, - inclusion of a wetting agent such as bovine serum albumin to help spreading of the specimen, - allow the grid to dry for at least 20 minutes (preferably at 45-60 C), - do not focus the beam on the specimens (causes boiling), - make sure you have a thin spread (not clumped), - offer a sacrifice to Pele or Vulcan.
Cheers, John
########################### Dr. John Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901 Phone: 618-453-3730 Fax: 618-453-2665 ###########################
UrAc is thought to be sensitive to light - especially UV type of light found in fluorescent light and sunlight. Causes breakdown of the stain and possible precipitation. Very little radioactivity is present - but some is there, so be careful.
JB
########################### Dr. John Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901 Phone: 618-453-3730 Fax: 618-453-2665 ###########################
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Long Liang wrote: ================================================ After a long use of the gold coater, the gold target is thinner and thinner and finally there is a hole showing up. I believe it is the time to replace the gold target.
My question is whether I can temporally cover the hole with a small piece of gold plate so I can still use the existing gold target, before I replace it with a new one. Any suggestions about how to connect two gold plates together (glue them with carbon paste ?). Thanks. ================================================= This is a novel suggestion. It might even work. However, there should be high conductivity between the "patch" and the rest of the cathode, and for that, we would use the same silver filled epoxy that we recommend when the cathode has to be glued onto the "head". Our prediction is that the carbon paste probably would not have sufficient conductivity. If silver paste was used, we would think that the silver would start to sputter away. When you do your "glue" treatment, make sure you put some weights onto the assembly so that you end up with a very uniform layer of the silver filled epoxy.
Disclosure: SPI offers all of the above discussed items as well as new "replacement" cathodes.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
This is a request for experienced users of Image Analysis software. I would appreciate if some of you are able to spare me some information on the types of software avaliable.
I have been using OPTIMAS at present, and I am interested in other programs which may be easier to use, and/or offer more capabilities. The ability to do FFT would be good.
I know also of "analySIS" and "SigmaScanPro" from thier web pages, but would also like to know some opinions of these from people who have tried the software.
I am also keen to find out prices of such software if they are availiable. I am looking at intermetallic particles in Aluminium Alloys, and currently using B/W backscatter images from the SEM.
We have made excellent experience with a three-axis system sold by
Integrated Dynamics Engineering 84 Cummings park Woburn, MA 01801 tel: 617-938-5120 fax: 617 938 5122 100125.1130-at-compuserve.com (address already mentioned)
The location of our FE-SEM (JEOL 6300F) is far from being ideal. There are a number of large machinery, elavators, printing machines etc next door.
Best regards, Dr. Peter Reynders +-----------------------------------------------------------------+ | Merck KGaA - Pigments & Cosmetics Division | | Bldg M 18, 64271 Darmstadt / Germany | +-----------------------------------------------------------------+ | e-mail: reynders-at-merck.de (work) reynders-at-compuserve.com (home) | | homepage: http://ourworld.compuserve.com/homepages/reynders/ | +-----------------------------------------------------------------+
Rozeveld, Steve (SJ) wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Another possibility: } } Integrated Dynamics Engineering } 84 Cummings park } Woburn, MA 01801 } tel: 617-938-5120 } fax: 617 938 5122 } 100125.1130-at-compuserve.com } } } Steve Rozeveld } } } } Dow Chemical Co. } } 1897 Bld. Door E-43 } } Midland, MI 48667 } } sjrozeveld-at-dow.com } } % (517) 636-5167 } } Fax: (517) 638-6443 } } } ---------- } } From: Ron Doole[SMTP:ron.doole-at-materials.oxford.ac.uk] } } Sent: Friday, June 12, 1998 2:08 AM } } To: Microscopy Community } } Subject: Re: SEM - problems with magnetic interference } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } On Thu, 11 Jun 1998, Alan Templeton wrote: } } Hi Alan, } } } } We have have been very interested in this problem as well. We have } } had trials of, and heard other good reports of, the field cancelling } } system sold by Oxford Instruments. Contact them at: } } Oxford Instruments, } } Research Instruments, } } Tubney Wood, } } Abingdon, } } Oxon. } } OX13 5QX. } } 01865-393200. Ask for Judith Brock. } } e-mail:- judith.brock-at-oxinst.co.uk } } } } Unfortunately I have no connections or financial interests. } } } } Good luck, } } Ron } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } Does anybody know of a manufacturer or supplier of magnetic field } } } containment loops. We are having problems with our Hitachi S-800FE } } } SEM - getting possible structurally transmitted magnetic interference } } } from lab two floors up. Our SEM is located in a basement with the } } } structural columns (steel reinforced concrete) directly above and to the } } } side of the SEM. Interference manifests itself in the form of CRT } } } screen "wobble" of set frequency especially noticable at high mag } } } (} x3K). Equipment in other lab determined to be the source of the } } } problem is a Microwave plasma reactor (interference occurs when } } } heater is on) and an Electro-cyclotron resonance (ECR) instrument. } } } Need urgent help.Cheers } } } Dr Alan Templeton } } } Centre for Physical Electronics and Materials } } } SEEIE } } } South Bank University } } } 103 Borough Road, London } } } SE1 0AA } } } TEL 44 171 815 7521 /7571 } } } FAX 44 171 815 7599 } } } email templea-at-sbu.ac.uk } } } } } } } =========================================================================== } } Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk } } Department of Materials, phone +44 (0) 1865 273701 } } University of Oxford, fax +44 (0) 1865 283333 } } Parks Road. } } Oxford. OX1 3PH. UK. } } ============================================================================ } }
We have a Phillips EM 420 TEM. It is getting on in years, but is still a lovely machine. We have a slight problem, though. The op-amp on the board for the auto-exposure system seems to blow after only two to three weeks use. We are not sure what the problem is and have been told by the local Phillips guys that ours is the only machine that does this. Short of trying to get a new/replacement board, are there any suggestions/plans/calls of sympathy out there? Any help will be appreciated. Thanks!
A colleague of mine is preparing x-sectional TEM specimens of CdZnTe/CdHgTe epilayers. We are using a new Gatan PIPS ion mill. The problem is that some specimens come out very nicely, but others are completely polycrystalline. The specimens are all prepared under the same/closely similar conditions, but we are unable to make reproducible TEM samples. I was therefore wondering whether there are any research facilities out there with a FIB machine that might have some time available on the machine to make some samples for us. Please advise as to the availability of the machine, and rates which apply for use, etc.
The Lynx Tissue Processor (now sold by Leica) is an instrument I have used for nearly 13 years-both here and at my previous employer. Operationally, the Lynx (and the RMC processor-a descendent of the old LKB processor) provides 20 stations that carry reagents up to pure resin. Both are carousel designs (with some differences) that have vials for reagents. I have used these for osmium through pure resin. A number of programs can be entered into memory, and the processing schedule tailored for the type of resin and the different dehydrants and en bloc staining protocols. It is theoretically possible to use the machine for immunocytochemistry or enzyme cytochemistry protocols, but I have not utilized them for those procedures.
The original instrument (purchased from Lynx before Leica took it over) was reliable and operated smoothly for the 4 years I was in that lab. I cannot give as ringing an endorsement to the unit we purchased here in 1990. Over the first 4 or 5 years, although there was low demand for use, we maintained the unit and did routine test runs. However, it has been necessary to send the unit back to Leica more times than I can (or care to) remember. It has run quite consistently for the last six months, during which time I have needed to process large numbers of samples daily for several weeks (I'd really rather not think about it). Hopefully, after nearly 8 years, the bugs have been worked out.
I have no experience with the RMC unit, and have been unable to get them to provide a demo unit for actual lab testing. That is frustrating. I wish you the best in your quest.
Roger Moretz, Ph.D. Dept of Toxicology
-----Original Message----- From: John N. Wright [SMTP:johnw-at-uts.cc.utexas.edu] Sent: Friday, June 12, 1998 2:40 PM To: Microscopy Community Subject: TEM Tissue Processors
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We are looking for a TEM tissue processor similar to the histological instruments that are available but one that would allow the automated (or nearly so ) fixation, dehydration, embedding and polymerization into various resins. I believe BAL-TEC used to make a freeze substitution unit that appeared to allow a number of resins and protocols, the FSU 010 but I can't find any sources of information regarding this or similar instruments. Any leads or comments would be appreciated.
Good timing. I have in front of me the latest copy of "The Microscope Book" (The Cambrex Group Mail Center, Suite 2, 112 Washington St, Marblehead, MA 01945-3554). There are three companies in this issue:
Fabreeka International, Inc. 1-800-FABREKA www.fabreeka.com {http://www.fabreeka.com}
Herzan 30251 Golden Lantern Laguna Niguel, CA 92677 949-363-2905 73071.3720-at-compuserve.com {mailto:73071.3720-at-compuserve.com}
TMC is the only one I know (personally) that makes anti-vibration units for EMs, but the others might be worth checking out.
Roger Moretz, Ph.D. Dept of Toxicology
-----Original Message----- From: Ron L'Herault [SMTP:lherault-at-bu.edu] Sent: Friday, June 12, 1998 8:02 PM To: microscopy-at-sparc5.microscopy.com Subject: vibration isolation tables
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We have moved our lab (groan) and the new site is not ideal for our scanning microscope. It looks like we need a vibration isolation table on the column section. I'm looking for recommendations for good vendors.
The first thing I would do is to check out the power supply for spikes and check grounding. Since the problem seems intermittant, it may prove difficult to "catch" it misbehaving. Another possibility may be inductive "kick-baqck". If there are any relays or solenoids associated with the circuit, a "snubber" diode across the coil may have gone open. That would permit a high voltqge spike to superimpose on the power supply.
In any case, you might try protective zener diodes. If the opamp uses +- voltage, one would be needed for each polarity feed. Choose a Zdiode with a rating a volt or two over the P.S. voltage. Connect from power feed to ground AT the opamp. Be sure to observe correct polarity.... If the added capacitance and the tiny leakage current will not be a problem, the same can be done for the inputs and output. If the I/O is bipolar, then two Zdiodes, wired in series - cathode to cathode should be used. The voltage rating of each these diodes should be slightly greated than the expected excursion of the I/O line it is protecting
Good Luck! Woody ------------------------------------------------ Woody White, Electron Microscopist SEM/EDS/WDS
Work: Mcdermott Technology, Inc. woody.n.white-at-mcdermott.com http://www.mtiresearch.com/
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Hallo All
We have a Phillips EM 420 TEM. It is getting on in years, but is still a lovely machine. We have a slight problem, though. The op-amp on the board for the auto-exposure system seems to blow after only two to three weeks use. We are not sure what the problem is and have been told by the local Phillips guys that ours is the only machine that does this. Short of trying to get a new/replacement board, are there any suggestions/plans/calls of sympathy out there? Any help will be appreciated. Thanks!
(SMTP Gateway for Microscopy-at-sparc5.microscopy.com); Mon, 15 Jun 1998 09:08:38 -0400 Message-Id: {199806151308.AA07916-at-gateway.ppg.com} Received: by gateway.ppg.com (Protected-side Proxy Mail Agent-1); Mon, 15 Jun 1998 09:08:38 -0400 Microscopy-at-Sparc5.Microscopy.Com
Hi Everyone, A long time ago (about 13 years) we used to use 3 1/4"X4" glass negative plates made by Kodak their catalog number was 185-5881. Maybe you could contact Kodak and inquire if they still make them. I hope this information helped. Jane Glamp PPG Inds.
Can someone please help Matija with this question?
} Date: Fri, 12 Jun 1998 11:37:17 -0400 (EDT) } From: Matija Maretic {matija-at-gravity.phys.utk.edu} } To: paul.perkes-at-asu.edu } MIME-version: 1.0 } } Dear Mr. Perkes, } } My name is Matija Maretic and I am doing research at University of } Tennessee at Knoxville. I know that Kodak carries a number of } scientific glass plates which can produce high quality positive image, but } I am wondering if they carry a plate which can produce a high quality } black & white negative. } Do you know where I can find this kind of information? } } Thank you, Matija Maretic }
Thanks,
Paul R. Perkes (602) 965-5218 Senior Applications Systems Analyst (602) 965-9004 FAX Center for Solid State Science (602) 952-9583 Wireless Arizona State University paul.perkes-at-asu.edu Box 871704 Tempe, AZ 85287-1704
You should try the small angle cleavage technique for these samples. No ion milling at all. You just need a delicate touch. Look up John McCaffrey and my paper in the TEM sample prep IV book from MRS, vol 480, 1997. This is a detailed, pictorial outline of how to do it. Send me your address and I'll send you a reprint. We are also going to have are poster at M&M98 on the technique. South Bay Technology has a kit that would cost you about the same as a day on an FIB.
What is the substrate? If it is a II-VI, and you use the technique, I would recommend a thickness of about 100-110 um for the final thickness. If you have problems, instead of the 15 degrees, you might want to go a little higher, say 18 degrees.
-Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
A colleague of mine is preparing x-sectional TEM specimens of CdZnTe/CdHgTe epilayers. We are using a new Gatan PIPS ion mill. The problem is that some specimens come out very nicely, but others are completely polycrystalline. The specimens are all prepared under the same/closely similar conditions, but we are unable to make reproducible TEM samples. I was therefore wondering whether there are any research facilities out there with a FIB machine that might have some time available on the machine to make some samples for us. Please advise as to the availability of the machine, and rates which apply for use, etc.
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} } The Lynx Tissue Processor (now sold by Leica) is an instrument I have } used for nearly 13 years-both here and at my previous employer. } Operationally, the Lynx (and the RMC processor-a descendent of the old } LKB processor) provides 20 stations that carry reagents up to pure } resin. Both are carousel designs (with some differences) that have } vials for reagents. I have used these for osmium through pure resin. A } number of programs can be entered into memory, and the processing } schedule tailored for the type of resin and the different dehydrants and } en bloc staining protocols. It is theoretically possible to use the } machine for immunocytochemistry or enzyme cytochemistry protocols, but I } have not utilized them for those procedures. } } The original instrument (purchased from Lynx before Leica took it over) } was reliable and operated smoothly for the 4 years I was in that lab. I } cannot give as ringing an endorsement to the unit we purchased here in } 1990. Over the first 4 or 5 years, although there was low demand for } use, we maintained the unit and did routine test runs. However, it has } been necessary to send the unit back to Leica more times than I can (or } care to) remember. It has run quite consistently for the last six } months, during which time I have needed to process large numbers of } samples daily for several weeks (I'd really rather not think about it). } Hopefully, after nearly 8 years, the bugs have been worked out. } } I have no experience with the RMC unit, and have been unable to get them } to provide a demo unit for actual lab testing. That is frustrating. I } wish you the best in your quest. } } Roger Moretz, Ph.D. } Dept of Toxicology } } } -----Original Message----- } From: John N. Wright [SMTP:johnw-at-uts.cc.utexas.edu] } Sent: Friday, June 12, 1998 2:40 PM } To: Microscopy Community } Subject: TEM Tissue Processors } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } We are looking for a TEM tissue processor similar to the } histological } instruments that are available but one that would allow the } automated (or } nearly so ) fixation, dehydration, embedding and polymerization } into } various resins. I believe BAL-TEC used to make a freeze } substitution } unit that appeared to allow a number of resins and protocols, } the FSU 010 } but I can't find any sources of information regarding this or } similar } instruments. Any leads or comments would be appreciated. } } Thanks in advance, } John N. Wright }
Margaret Springett e-mail hukee.margaret-at-mayo.edu IEM Specialist at Mayo Foundation 1426 Guggenheim Rochester, Mn. 55905
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Dear fellow microscopists and friends, At San Joaquin Delta College, Microscopy Technology Center, we are a = 2 yr microscopy training program, as many of you know. Recently we = posted a position for an Adjunct for Teaching Fall semester 1998 while I = take a sabbatical. Unfortunately the person that signed the contract = for it, decided Friday that he no longer wants to do it. This leaves us = in a real bind. If any of you have ANY ideas for getting these four courses taught = this fall (the position info is listed below with the courses) in = Stockton, CA, please e mail me directly. If you want to apply, contact = Human Resources directly at 209/954-5056 for an application. Perhaps you know someone who is just finishing a post doc and might = like it for the semester, or someone who could take a leave for a = semester from industry or educational institution. OR perhaps you know someone who could teach one of the courses and = is nearby. We are open to any kind of suggestion at this point. The job = offers full benefits and a good salary for the semester. The courses would not require development of new courses as these = courses have been fully developed. Course materials are available to aid = in the instruction. My sabbatical is locked in for Fall as I must beat the bushes to make = $3 million for our new building to match the state's funding of $4.5 = million. Any ideas are welcome. If you could teach the courses, you can get an = application at Human Resources whose phone number is listed below. Any help is appreciated. If this note sounds a bit desperate, IT IS. = Time is of the essence.
Thanks Judy Murphy
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
URGENT: ADJUNCT FACULTY NEEDED FALL Semester 1998 (Aug. 12 - Dec 18, 1998)
A San Joaquin Delta College Faculty member of the Microscopy Technology = Center is planning a Sabbatical Leave for the Fall '98 semester. San = Joaquin Delta Community College is currently searching for an EM = Instructor Adjunct / Temporary One Semester Contract for Fall 98 (Aug. 12 = - Dec 18, 1998).
MINIMUM QUALIFICATIONS: Bachelor's Degree plus two years of directly related experience OR an = Associate Degree plus six years of directly related experience OR a valid = credential.
DESIRABLE QUALIFICATIONS: Master's or PhD Degree in a Biological = Science; Experience in teaching / practice of Electron Microscopy
COURSES TO BE TAUGHT:
Introductory Techniques for Transmission Electron Microscopy (EM21) This is a lecture/lab course which includes beginning Transmission = Electron Microscopy dealing with the alignment and operation of the TEM, = vacuum techniques, photographic techniques, as well as the preparation of = particles and replicas for viewing in the TEM. Includes individual = training in the use of the TEM, preparation techniques, and written and = oral reports. (Lec - 2 hrs; Lab - 3 hrs/wk)
Biological Ultrastructure (EM28) Course contents include specific information about the fine structure and = function of cells and tissue at the ultrastructure level. Videos, slides = and micrograph examination will be correlated with the lectures so that = students will learn to recognize the fine structure of cells and tissues = in relationship to their function. (Lec-2 hrs/wk)
Advanced Techniques in Biological Electron Microscopy (EM37) Course contents include lecture and laboratory which covers advanced = techniques for biological specimen preparation in TEM including an = advanced research project.( Lec - 1 hr; Lab - 6 hr/wks)
** Current Microscopies: Optics, Theory and Application (EM30) Course contents include information related to the physical laws and = applications of the various types of current microscopies e.g. = TEM,SEM,FIB, AFM, and confocal microscopy, as well as other current = topics e.g. asbestos analysis, lab design, etc. (Lec - 2 hrs; Lab - 3 = hrs/wk) ** This course is negotiable. A portion of this assignment would be = paid as an additional overload.
The above teaching load would not require development of new courses as = these courses have been fully developed. Course materials are available = to aid in the instruction. TERMS OF EMPLOYMENT: Full semester, Non-tenured track position with Full = Benefits (Medical, Dental, & Vision). SALARY for Fall 98 semester: BA/BS w/ 2 yrs experience or AA w/ 6 yrs experience$17,498 - 25,333 for Fall 98 sem
MA/MS$19, 219 - 28,137 for Fall 98 sem
PhD$ 22,528 - 31,739 for Fall 98 sem
APPLICATION: Contact Human Resources at 209/954-5056. Human Resources San Joaquin Delta College Admin. Bldg. Rm 202 5151 Pacific Ave Stockton, CA 95207
DEADLINE: Please send your cover letter of application, an application = form for employment, and copies of all transcripts as soon as possible. = The Screening Committee plans to begin the review of applications on June = 29, 1998.
Additional SJDC Microscopy Technology Program Information available at = http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html/
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Dan Caruso asked:
{Are there any advantages to using cacodylate buffers in fixatives as {opposed to phosphate buffers? Are there any disadvantages in addition to {the toxicity of cacodylate buffers?Specifically, I am working with human {corneas. Thanks for your replies.
One of the reported advantages of cacodylate buffers - Andrey Glauert dixit - is that they do not support microbial growth, e.g. fungi. This is almost true. Fungi can actually grow in glutaraldehyde fixed tissue 'eating up' the fixed protein, and traveling throughout less dense spaces, sometimes giving images suitable to illustrate science-fiction 'infections'. We reported this some years ago in
Growth of fungi in cacodilate buffer - J.F. Moura Nunes, A.P. Aguas e J.O. Soares - Stain Technology, 55: 191-192, 1980
Best wishes
J.F.Moura Nunes Lab. Electron Microscopy Portuguese Cancer Institute 1093 Lisbon Codex Portugal
I teach two upper level classes Plant Anatomy & Structure and Function of Algae and Land Plants. I am constructing web pages for them. These can be seen from my home page www.botany.hawaii.edu/faculty/webb/default.htm
I need EM images, especially for plant and algal cell ultrastructure. All algae are welcome. However, SEM images would also be welcome. I would like to post these on our server so that they could be accessed via the Internet. Authors would be credited. I have my own digitizer. Thus, you could send me a photographic slide or negative (color OR black and white) as well as Prints!!!! Otherwise, I could send you a ZIP Disk or Floppy Disks which you could use to transfer files. I will pay postage!!! Finally, I could download them from a web site. Feel free to use any of my images. I am working on Plant Anatomy this summer and that site will be expanding rapidly during July. Finally, if you know of any good websites for the above, please let me know.
In response to the recent discussions concerning victawet we'd like to make the following case:
Ladd has manufactured Vacuum Evaporators for the EM field for over 30 years. Since each one has to be factory tested upon completion it's important that we are able to make them spotless again prior to shipping After trying to accomplish this for years we settled on the following procedure:
1. Evaporate victawet from a tungsten basket which coats the bell jar
2. Test Evaporator
3. Using a soap solution and lint free cloths we then wipe down the internal parts.
This has worked well for us for years.
SUBSTRATES
We periodically do special substrates for some of our customers who insist they be as contamination free as humanly possible. To accomplish this we use victawet prior to the substrate run. This assures the elimination of any possible residual oil.
John Arnott LADD RESEARCH 13 Dorset Lane Williston, VT 05495
***************************** DISCLAIMER **************************** Ladd Research sells victawet, vacuum evaporators, bell jars, substrates and other products mentioned in this e-mail
I am about to begin a phase of a research project involving microtome sectioning of flowers and fruits for developmental studies. I am interested in finding any educational cd's, vcr's, or other resources on the process of rotary microtome sectioning. I have several books and "human" resources for guidance but it would be helpful if there are any other visual or lab teaching aids out there as well.
Thanks in advance for any leads or ideas.
please respond to: crow-at-aloha.net
_______________________________________________________________ Melany H. Chapin
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After dark, all cats are leopards. (Zuni proverb) We will be known by the tracks we leave. (Dakota) When the legends die, the dreams end; there is no more greatness (Shawnee) Every animal knows more than you do. (Nez Perce) ________________________________________________________________
I don't have the schematics of that particular instrument handy, but here's a simple tip. If there are any transistors being driven by this opamp, replace them. Often in repairing a problem, a failed component will be replaced and the circuit will operate, but related components in the circuit may have been stressed to the point where they make excessive demands on the replaced component.
Another situation arises where a component may get marginal in operation, such as a transistor suffering from thermal runaway. That component may not fail but may cause others to fail. Replacing a component earlier in the circuit may correct the apparent problem, but not the cause - the replaced part will soon require replacement again.
} Hallo All } } We have a Phillips EM 420 TEM. It is getting on in years, but is } still a lovely machine. We have a slight problem, though. The } op-amp on the board for the auto-exposure system seems to blow after } only two to three weeks use. We are not sure what the problem is } and have been told by the local Phillips guys that ours is the only } machine that does this. Short of trying to get a new/replacement } board, are there any suggestions/plans/calls of sympathy out there? } Any help will be appreciated. Thanks! } } Regards } Alistair Douglas Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-mcs.net WWW: http://www.mcs.net/~ars Analytical instrument maintenance services
Many thanks to everyone who replied to my query about non-bubbles with negative staining. The replies confirm what I'd always suspected lo these more than 20 years at EM - it's not just me, no one knows what's going on with some of this stuff. It's all witchcraft.
ohn Bozzola has called the effect Champaigning, which is a lovely description. Many people sent me their pet ideas about what causes the effect, and what to do about it. I can debunk most of them, usually in the course of one staining session. Sigh.
I get it with UrAc, PTA, and uranyl formate. I don't carbon coat my grids, so hydrophobic carbon is not the culprit. There was no glycerol in the sample. Wetting agents have not helped. I can get good spreads, and last week one of the grids was gorgeous, while the other 19 spread well but had bubbles. Happens with all kinds of samples.
The main difference with the one that worked was that it dried longer while I babbled on atthe people I was training. I will pursue this line of investigation.
Sacrifices to Pele generally help, but already the microscope room is full of ti leaves. (Even our engineers get into this.)
As for my other uranyl acetate question- I know UrAc is light sensitive, and so I have always kept it in a dark bottle in the fridge where presumably the light is out when the door is closed. I can't confirm this. Anyway, for the stuff left out in room light, I had always wrapped the container with aluminum foil. I keep stain in 10 cc syringes with millepore filters on them at room temp so they are always ready for staining. I recently read that foil was a bad idea, but with no explanation. Phil Oshel offers one-
"It's worse than you think. UAc is afraid of the dark. When wrapped in foil, it starts shaking with fear, and the nuclei go into resonance, lowering the nuclear-shell transition energies. More neutrons are released, leading to a chain-reaction.
This is know as phobiafusion, and was discovered by a group of unemployeed physicists and molecular biologists in San Fransico who had set up as macromolecular psychotherapists."
It made me laugh.
Aloha, Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Does anyone have a fax or email address for a supplier of electronic components who's likely to be able to supply me with 6 or so Burgess microswitches type V4T7-GP ? Local agents have a minumum order of 50!
cheers
Ritchie
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
I recommend Newport Micro-Controle. Sorry, I don't know their address in the US since I dealt with their Belgian representative. They custom designed a table for my LEO/Zeiss EM912 which works perfectly. I should also point out the excellent contact and customer service I received.
Usual disclaimer: no personal interest in this company, I'm just a very satisfied customer.
Regards, Michel
**************************************************** Michel Deschuyteneer, Ph.D. deschuyt-at-sbbio.be Scientist Electron Microscopy Laboratory
SmithKline Beecham Biologicals Rue de l'Institut, 89 B1330 Rixensart, BELGIUM Tel: +32-2-656 9290 Fax: +32-2-656 8164 **************************************************** Standard disclaimer: the opinions expressed in this communication are my own and do not necessarily reflect those of SmithKline Beecham. ****************************************************
Hi, I have a question on the older NIM bin Ln2 sensor / bias protection modules Tracor Northern used. Module 1236. Does someone know what device they used as the sensor that went in the Ln2. Was it a diode or a thermistor of type ? We have the module that we would like to use on another system, but have no reference to the sensor device.
Thanks Luc Harmsen Anaspec, South Africa International technical support on microscopy. Tel: +27 (0) 11 476 3455 Fax:+27 (0) 11 476 7290 anaspec-at-icon.co.za
} } To: {FreidaC-at-aol.com} } From:tflore-at-lsumc.edu (Teresa Flores) } Subject:Re: Spanish unite with NSH } Cc:histonet-at-pathology.swmed.edu } } Frieda, thanks for all your suggestions and your genuine concern. } I thought that you knew that there was is a Sociedad de Latinoamericana de } Histotecnologia (SOLAHT), of which, I am the President and Rosemary } Velasquez is the Secretary-Treasurer. SOLAHT was founded in 1995 in Santa } Cruz, Bolivia. There are approximately over 300 members and over 100 that } I have not had an opportunity to imput or respond to (because of personal } commitments and the tremendous amount of work I have right now). These } members range from Mexico, Guatemala, Panama, Costa Rica, San Salvador, } Nicaragua, Honduras, Bolivia, Ecuador, Paraguary, Argentina, Chile (I } might have left out a country or two). I did not realize how time } consumeing it would be to keep up with the SOLAHT and sometimes I am } overwhelmed. } I believe I will send out a communication to the SOLAHT with information } on becoming an NSH member. I would need NSH International yearly dues and } what they would include. Also, if SOLAHT members want to become NSH } members and not receive the Histology journal, is there a lesser NSH fee? } Or if SOLAHT members just want the Histotechnology journal, is there a } lesser NSH fee? } Frieda, please give me all the particulars and options so that I may relay } the information to SOLAHT members. } In the communication to SOLAHT I will be requesting e-mail addresses to } try and begin a listserver. } Thanks again for all your help. Teresa. } ,} Theresa: } } } } There is no provision for corresponding members in NSH. All members outside } } of the US and Canada are International members and they pay the same dues and } } receive the same benefits as the regular active members. } } } } Hard Tissues do not have an extra day. There are special workshops and } } seminars especially slanted toward the VIR or hard-tissue group, but some are } } attended by clinical members. } } } } We would have to have a guarantee of a certain number of Spanish-speaking } } attendees in order to make special concessions. Rather than an extra day, } } translators might be a better choice. However this would probably cost a lot } } more and an extra charge would have to be made. I do not know how we could } } guarantee attendees either. } } } } If there is as much interest as you say, has there been any thought given to } } South and Central American and Mexico forming a Spanish-speaking Society? } } } } I know I am not being much help - but I do not know what to suggest. } } } } Freida }
To all MSA/MAS '98 meeting attendees (esp. SEMS, AREMS and AMS members), If you don't want to see Centennial Olympic Park or be sippin' mint juleps on the veranda we could use your help staffing the Information and Hospitality Booth. Cynthia Goldsmith and I are coordinating the Booth. If you have a few hours to spare please let me know. No prior experience is necessary and I guarantee your pay stub will have a lot of zeros in it :-).
Thanks for considering this matter. See you in Atlanta, Beth Richardson
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
The Biological Sciences meeting of MMMS will be held on Wednesday, July= 1 at the Blood Research Institute in Milwaukee, Wisconsin. Registration and=
Poster Set-up begin at 10:00 a.m.; the first scientific presentation st= arts at 10:30 a.m.
Registration is free to current members, and attendees may join the soc= iety at the meeting. Dues are $10 for regular members and $5 for students. = No pre-registration is required to attend the scientific sessions; however= , if you wish to have lunch with us, we DO need a headcount and menu selecti= ons by June 26. YOU MUST RETURN YOUR MENU SELECTIONS TO US, IF YOU WANT TO PARTICIPATE IN LUNCH!
The menu, complete agenda, and travel instructions will be sent to curr= ent MMMS members this week. For anyone else interested in attending, pleas= e contact Linda LaRonge-Snow at linda.snow.nmi-at-abbott.com OR telephone (847) 937-5251 OR fax (847) 93= 8-5027 with a fax number or mailing address (fax preferred).
Poster space is available for anyone wanting to show off their work. Available space for each poster is 3 X 5 feet. We'd like to have a rou= gh estimate of the number of posters, so please contact Jim DiOrio at diorio-at-baxter.com if you intend to display a poster. Last minute entr= ies, however, will be accepted!
The meeting agenda is as follows:
10:00 - Registration and Poster Set-up
10:30 - Using Correlative Microscopy in Biological Problem Solving; Ral= ph Albrecht, Ph.D., Univ. WI - Madison
11:15 - Localization of C-Terminal Gamma Chain Regions in Gold-Cadaveri= ne Labeled Fibrinogen by STEM; Michael Mosesson, M.D., Univ. WI Medical = School
Noon - Lunch and Poster Session; Medical College of Wisconsin
1:00 - Digital Manipulation of Acquired Images: "What Is Possible vs. W= hat is Ethical"; John Kinnamon, Ph.D., Univ. Denver; MSA/LAS sponsored speake= r
1:45 - Roses and Feathers; Joseph Harb, Ph.D., Diagnostic EM, Med. Coll= ege of Wisconsin
2:30 - Microscopic Characterization of T-DNA Tagged Male-Sterile Arabid= opsis thalianaLines; Heather Owen, Ph.D., Univ. WI - Milwaukee
3:15 - Refreshments
3:30 - Digital Imaging Workshop - John Kinnamon, Ph.D.
For further information, you can contact me at jane.a.fagerland-at-abbott.= com. Hope to see you in Milwaukee on July 1st!
Dear Tina, } } I remember reading somewhere that we should not wrap contianers of uranyl } acetate in aluminum foil. Why is that? Is there a danger of } bremstrunghumuhumunukunukuapuaa radiation? (Sorry, it's Friday afternoon } and I clearly don't know what I'm talking about.) } I am not sure why one wouldn't wrap UAc containers in Al foil, but it has nothing to do with radiation. The only kind of radiation which will penetrate the bottle to interact with the foil would be gammas from some of the daughters of the U. If one had a very thin-walled container and a very energetic beta, one might have to worry about brehmsstrahlung radiation (It's Tuesday morning & I'm still awake), but since its production goes as a power of Z, Al is one of the better metals for avoiding this problem. Be is the best mechanically sound metal for avoiding brehmsstrahlung, but has other problems. Plastic is another good material for avoiding it. Yours, Bill Tivol
Phobia*fission*! Sorry. I was so worried about the effects of a small nucleopsychotic reaction in Tina's 'fridge that I mixed up my fused and split personalities.
Phil
} As for my other uranyl acetate question- } I know UrAc is light sensitive, and so I have always kept it in a dark } bottle in the fridge where presumably the light is out when the door is } closed. I can't confirm this. Anyway, for the stuff left out in room } light, I had always wrapped the container with aluminum foil. I keep } stain in 10 cc syringes with millepore filters on them at room temp so } they are always ready for staining. I recently read that foil was a bad } idea, but with no explanation. Phil Oshel offers one- } } "It's worse than you think. UAc is afraid of the dark. When wrapped in } foil, it starts shaking with fear, and the nuclei go into resonance, } lowering the nuclear-shell transition energies. More neutrons are } released, leading to a chain-reaction. } } This is know as phobiafusion, and was discovered by a group of unemployeed } physicists and molecular biologists in San Fransico who had set up as } macromolecular psychotherapists." } } It made me laugh. } } Aloha, } Tina } } http://www.pbrc.hawaii.edu/bemf/microangela } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } ****************************************************************************
}}}}}}}}}}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{{{{{{{{{{ Philip Oshel PO Box 5037 Station A Champaign, IL 61825-5037 (217) 355-1143 oshel-at-shout.net or poshel-at-hotmail.com ***** looking for a job *****
The real reason for not using aluminum foil is that the acetate ion will decompose the aluminum (if liquid dribbles down the side of the container --- gasp --- and onto the foil). This generates a crumbly mess that is contaminated with Ur to boot. If one carefully pipettes the UrAc, the contact is avoided. We have used aluminum to cover our UrAc for several decades and only once did the Al decompose (in out student laboratory).
} } I remember reading somewhere that we should not wrap contianers of uranyl } } acetate in aluminum foil. Why is that? Is there a danger of } } bremstrunghumuhumunukunukuapuaa radiation? (Sorry, it's Friday afternoon } } and I clearly don't know what I'm talking about.) } } } I am not sure why one wouldn't wrap UAc containers in Al foil, but } it has nothing to do with radiation. The only kind of radiation which will } penetrate the bottle to interact with the foil would be gammas from some } of the daughters of the U. If one had a very thin-walled container and a } very energetic beta, one might have to worry about brehmsstrahlung radiation } (It's Tuesday morning & I'm still awake), but since its production goes } as a power of Z, Al is one of the better metals for avoiding this problem. } Be is the best mechanically sound metal for avoiding brehmsstrahlung, but } has other problems. Plastic is another good material for avoiding it.
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy Neckers Building, Room 146 - B Wing Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
The TN 1236 Bias Protection Module was actually designed and built by Tracor X-ray which is now Spectrace Instruments, 1275 Hammerwood Avenue, Sunnyvale, CA 94089 USA. phone 408-744-1414 www.spectrace.com Wayne Watson or Bill Drummond could help you on this. I believe the sensor was a thermistor. They may still use the same sensor in their XRF spectrometers with LN detectors.
Ronald Vane XEI Scientific
Luc Harmsen wrote: } } Hi, } I have a question on the older NIM bin Ln2 sensor / bias protection modules Tracor Northern used. Module 1236. } Does someone know what device they used as the sensor that went in the Ln2. Was it a diode or a thermistor of type ? } We have the module that we would like to use on another system, but have no reference to the sensor device. } } Thanks } Luc Harmsen } Anaspec, South Africa } International technical support on microscopy. } Tel: +27 (0) 11 476 3455 } Fax:+27 (0) 11 476 7290 } anaspec-at-icon.co.za
I have been asked to recommend a stereo light microscope and camera combination for use in a classroom situation. I turn to you, the experts, for suggestions, advice, and ideas.
The microscope will be used in a plankton class to record images of 'things'. The pictures will be used to share with other students in the class so everyone can see the 'things' everyone else collected. The pictures might also be used in student reports and possibly made into 2x2 slides for teaching. Images of the highest quality are not required, but they should be reasonably good.
I am not familiar with the latest in stereo microscopes so some pointers here would be helpful. Are there any models that stand out as particularly good candidates for this type of application?
For this application I suggested some sort of digital camera and printer. Could a Pixera system fulfill the needs of this user? How about an inexpensive color printer to have nearby?
At the moment, there is no budget for this project. When I asked 'How much is available?', I got "Well, I don't know, but, you know, there is always some end of the year money that we have to spend".
Sooooo, hit me with your best shot. I am thinking of a simple stereo scope, a good fiber optic light source, a simple digital camera, and a simple digital printer for 3" x 5" pictures. It will probably have to be on the inexpensive side and fairly bulletproof.
If you have any experience with specific combinations of components that worked well or turned out poorly, let me know and I will pass your info. on to the interested folks here.
Thanks again for your thoughtful contributions.
Jonathan Krupp Microscopy and Imaging Lab University of California Santa Cruz, CA 95064 (408) 459-2477 FAX (408) 429-0146 jmkrupp-at-cats.ucsc.edu **Area code changing to 831 as of 7/11/98**
This is a News Release from CASIX, Inc. CASIX will send it to you every two weeks to announce recent advances taken by CASIX in the photonics field as well as important event related. If you want to unsubscribe from the mailing list, you may simply turn back this with subject unsubscribe.
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(1.38.193.4/16.2) id AA06588; Wed, 17 Jun 1998 18:48:30 +0300 Message-Id: {3.0.5.32.19980617184140.008a8740-at-zeus.csd.auth.gr} X-Sender: info-at-zeus.csd.auth.gr X-Mailer: QUALCOMM Windows Eudora Pro Version 3.0.5 (32)
Visit our website and download a free demo of our new software
Recently there has been interest in providing TEM or SEM pictures of single celled creatures for use in educational websites, etc. But while today we have access to wonderful imagery undreamed of by our forbears, we should not overlook the wonderful achievements of the optical microscopists of the 19th century. So if you want:
DESMIDS, DIATOMS or RADIOLARIANS
I cannot suggest better than "ART FORMS IN NATURE" by ERNST HAECKEL, one of the greatest of the 19th century microscopists. This book of his drawings was published by Dover in 1974. All sorts of creatures, laid out in beautiful symmetric patterns (Maurits Escher, eat your heart out!) How high Haeckel's reputation was can be seen in this quote from "Life and its Beginnings" (1914) by Dr Helen Webb:
"There is really no end to the wonders which can be told of the varieties of little one-celled creatures. I can here give you only a few examples of them and their peculiarities. If we want to know more, as we all do, there are numbers of interesting books written about them which we can read. Even when the books themselves are very difficult the pictures are delightful to look at. Some of the loveliest are to be found in Haeckel's books, and if ever you have an opportunity I advise you to try and draw some of them.
O Lord, how manifold are Thy works ! In wisdom hast Thou made them all: ...."
There is really an irony here, because in Dr Webb's book we have a good Christian lady writing a gentle introduction to the facts of reproduction, while Dr Haeckel was a colourful and controversial character, notorious for his outspoken atheism - so much so that whenever he visited Darwin at Down House, everyone was on tenterhooks in case he came out with something over-the-top.
But Haeckel's contribution to microscopy was far greater than simply being a superb artist. Most notably, he brought to attention the features of embryonic development, for example the transitory gill-slits that appear in mammalian embryos. This he tended to over-interpret, as if the embryo were to go through a "fish stage", then a "reptile stage", before "evolving" into a mammal. The doctrine was stated as:
"Ontogeny recapitulates Phylogeny".
Haeckel has been recently criticized for seeing and drawing too much of "conserved stages" in embryonic development - in other words of seeing what he wanted to see under the microscope (long before the days of image manipulation!) On the other hand, it is argued that he got the broad picture right, and the details were sorted out later. I would say there is truth in both sides. Even with my own humble polymer spherulites, I realize that the developments I have seen under the microscope were not as regular as I at first saw or thought.
Anyway, do look at these drawings. So many "handbooks of pond life" published since must owe something to them. But I am sure that these micro forms have had an influence far beyond science, and that countless science fiction covers could trace back their ancestry to this source. But there is something even more - one is really seeing the micro world through the eyes of the 19th century. Looking at these drawings gave me a slightly vertiginous feeling, as if I had entered a fold in space-time and was really looking directly into the world of our Victorian ancestors.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
Absolutely daft - but I have to ask you-all if anybody knows a source of copper gauze, so that we can make the kind of baskets on which we place grids for extracting replicas?
UK supplier would be most convenient, but I don't mind looking across the Channel or the Pond.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
Micro-tools' phone number is (408) 395-1585. The part is Cat. #S-078-B and they call it a fine point diamond scribe. They should be considered expendable. This is the part number that Stacie Kirsch uses at Electron Microscopy Sciences also. I don't know what the South Bay Technology's number is. Get a minimum of 25 if you think you are going to use the technique regularly or maybe ten to try it out. When they break, you can use them for marking and for the coarse scribing step on the back side of the sample. You really need good sharp ones for the final cleaving step. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
----------
There is a company called Buckbee Mears that sells and makes all types of etched and electroformed meshes and masks in different sizes and materials. They will make items to order. Sorry, I don't have the number or the address. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Absolutely daft - but I have to ask you-all if anybody knows a source of copper gauze, so that we can make the kind of baskets on which we place grids for extracting replicas?
UK supplier would be most convenient, but I don't mind looking across the Channel or the Pond.
+----------------------------------------------------------------------- -+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley |
A short time back someone inquired about vibration isolation systems for EMs. With that in mind, I would suggest that you contact the following company:
Minus K Technology 420 S. Hindry Ave. Unit B Inglewood, CA 90301
They have some really great products. I have no financial interest in th= e product or the company - I've just seen the products and think they're pretty nifty.
To the CLSM users, Is there anyone out there using the Noran Oz Confocal system, and is doing at least double (hopefully triple label) fluorophore detection and are experiencing the following bugs: 1)If you shut down acquisition, the previous slit numbers are no longer optimal when you restart acquisition. 2)If you change the slit position numbers, the image shifts slightly (triple 0.5 um fluorescent bead overlays confirm this), which means recalibration almost everyday. 3)Uneven illumination across the field (seen especially with sections that encompass the whole field. 4)Incompatible astigmatisms especially the 633nm or cy-5 5)RF noise as seen as a horizontal line on the right hand side when the backround is high. These are our major concerns and eats up a lot of my time with constant recalibrations and checks. The company claims this is not unique to our system and are working on the problems. The also claim no one is using the system to the full capacity like we are (ie double and triple label). Has anyone seen the last demo at Woods Hole Mass? The system supposedly had a good showing. Did they use 0.5 um beads or huge pollen grains for demo. Did they do double and triple label imaging? Am I alone in my problems or are there others out there? Let me know.
Following up on an earlier posting: the Buckbee-Mears company referenced as a supplier of etched meshes etc is located in St. Paul, Minn and their phone number is 612 228-6434. -- Fred Schamber ....................... mailto:fhscham-at-SGI.NET
Hello All, I am interested in replacing an existing heating stage, (Leitz 350) which has served us well for } 10 years. If anyone has experience/comments on an appropriate replacement, ie. heating to 350 - 500C and will fit a Zeiss Axioplan microscope I'd like to hear from you.
Hi everybody, =20 we need a glow discharge unit to render carbon-coated grids=20 hydropohilic prior to negative staining. Does anyone have a) a machine= =20 which is not in use any longer that could be purchased at a reasonable= =20 price, or b) informations about companies still producing such=20 devices?=20 =20 Many thanks in advance for help, Matthias
Thank you. We at Electron Microscopy Sciences do sell Glow Discharge units stand alone as well as attachments to carbon coating units.If you send us your address we will be more then happy to give you all of the information on the units we have to offer. Thank you.
POSTDOCTORAL RESEARCHER IN ELECTRON MICROSCOPY OF DEFORMED ROCKS
Initial salary within the range =A315,462 - =A317,266 pa=20
A NERC-funded post is available for 2.5 to 3 years, from 1 October 1998 in = the=20 application of frontier electron microscopy techniques to examining deforme= d=20 rocks. In the Microstructure Research Group we have pioneered the use of=20 electron backscatter diffraction patterns, SEM orientation contrast imaging= and=20 TEM to characterise geological deformation microstructures and hence mechan= isms=20 and rheology. The post would suit geologists, materials scientists and=20 physicists with experience of electron microscopy and the ambition to devel= op=20 innovative techniques with applications within and outside Earth Sciences.
Informal enquiries to Dr J Wheeler email: johnwh-at-liv.ac.uk or Dr D Prior em= ail:=20 davep-at-liv.ac.uk. Web site=20 at http://www.pcweb.liv.ac.uk/johnwh/microstructure.html=20
Quote Ref: B/950 Closing Date: 17 July 1998=20
Further particulars and details of the application procedure may be request= ed=20 from the Director of Personnel, The University of Liverpool, Liverpool L69 = 3BX=20 on 0151 794 2210 (24 hr answerphone) or via email: jobs-at-liv.ac.uk=20
Web site at http://www.liv.ac.uk=20
John Wheeler Dept. Earth Sciences, Liverpool University, Liverpool L69 3BX, U.K. Email: johnwh-at-liverpool.ac.uk Fax: 0151 794 5170 Telephone direct: 0151 794 5172 Departmental home page on http://www.liv.ac.uk/earth_sciences/dept/general.html
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There have been requests for a summary of replies I received regarding image analysis packages. I usually delete e-mails shortly after receiving them, but here is the summary based on hard copies of e-mails I printed (recall that my application was grain sizing, so I may have disregarded packages that had primarily biological applications):
Image Pro Plus with Materials Pro plug-in: http://www.mediacy.com Princeton Gamma-Tech's Imagist: http:// www.pgt.com John Russ' Image Processing Toolkit: http://members.aol.com/ImagProcTK/index.htm Soft-Imaging Software Corp.: http://www.soft-imaging-web.de/products EMS On-Line: http://cimewww.epfl.ch/ Impuls's Vision XL/XXL: http://www.datex.de KS series from Zeiss: http://www.imas.co.uk/rep/kont/3czv.htm MetaMorph from Universal Imaging: http://www.image1.com Scott Scientific: http://www.scottscientific.com/ccad.htm Northern Eclipse from Empix Imaging: http://www.empix.com
Both Leica and Leco offer image analysis packages as well.
I also disregarded replies related to hardware, since we are only looking at software.
Hope this summary is useful.
Nancy Zjaba National Semiconductor South Portland, ME
by smith.edu (8.8.6/8.8.6/SC1.6) with ESMTP id IAA20050 for {Microscopy-at-Sparc5.Microscopy.Com} ; Thu, 18 Jun 1998 08:03:04 -0400 (EDT) Received: from SCIENCE/SpoolDir by science.smith.edu (Mercury 1.31); 18 Jun 98 08:03:05 EST Received: from SpoolDir by SCIENCE (Mercury 1.31); 18 Jun 98 08:03:03 EST
You can build on yourself quickly and inexpensively. See:
Aebi and Pollard, 1987, Journal of Electron Microscopy Technique 7:29-33.
We made one a number of years ago; I use it fairly often and it is terrific for just the task you mentioned. And when not in use in the glow discharge unit, the Tesla coil is fun to play with, but you need to be careful.
On Thu, 18 Jun 1998 matthias.morgelin-at-medkem.lu.se wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi everybody, } } we need a glow discharge unit to render carbon-coated grids } hydropohilic prior to negative staining. Does anyone have a) a machine } which is not in use any longer that could be purchased at a reasonable } price, or b) informations about companies still producing such } devices?
Matthias,
We bought a HDT200 `hydrophillic treatment device' from JEOL. It is simple to use and reliable. We use it to help prevent specimen contamination under the beam but it seems to be what you are after. I am unsure of the present cost but it was significantly less than other plasma devices available at the time.
I have no personal interest except as a satisfied customer.
Regards, Ron =========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
} Position: SEM Operator / Lab Technician } } Oz Technologies is a leading edge supplier of Test Interface products } for the Semiconductor industry, located in Hayward, CA. } } Description: } } Research and Development lab technician to perform variety of lab } related functions. Self-starter & Fast learner with an inquisitive and } exploratory mind to assist Engineers in Development of new products. } } Responsibilities: } } * Preparation and Cataloging of samples for SEM/EDS analysis, } * Executing Design-of-Experiments, } * Metrology assessment and Data Collection } * Administering Equipment (Operation, maintenance, calibration, etc.) } * Report generation and archival } } Skills required: } } * Proficient in running SEM (Variable Pressure) and EDS systems } * Proficient in cross-sectioning equipment, microhardness testers, etc. } * Ability to understand DOEs and instrument setup to execute } experiments } * Proficiency in computer skills (data collection, analysis, reporting) } is a must } * 4+ years of direct experience is desired } * Material Science (Metals, Polymers, Electro-Chemical) background is } a plus } * Measurement and Instrumentation background is a Plus } * Capability in handling multiple tasks with short turn around time. } } Organization } This position reports to Engineering Organization } } Additional notes: } Oztek is a dynamic, fast-paced company. As a result, employees are } periodically asked to perform duties outside the defined scope of their } position. We are a high tech company that manufactures } test and reliability products for the semiconductor, test and burn-in } industry. } Please visit our website for additional information about our company: } www.ozcorp.com } } If you are interesting in this position, please email me your resume. } Oztek reserves the right to change job functions as needed to meet } staffing requirements. }
} we need a glow discharge unit to render carbon-coated grids } hydropohilic prior to negative staining. Does anyone have a) a machine } which is not in use any longer that could be purchased at a reasonable } price, or b) informations about companies still producing such } devices? } } Matthias
You can do this cheaply and easily with a $50 Tesla coil. Arrange a good electrical contact with an unused feedthru in the baseplate of your evaporator, and discharge the coil during the rough pump part of the pumpdown. Or you can mount the coil on a vacuum dessicator and pump it with lab vacuum or a small rotary pump.
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/
Several weeks back I requested information that would help educate the administration regarding the realities of "cost neutrality" at University microscopy facilities. I received about 35 responses 24 of which provided some information about how their labs were doing, the remaining 11 were interested in having a copy of the responses.
First, I am very grateful to those who took the time and responded. The compiled responses had a favorable impact on how some of the administration at my University perceived such a facility should be reasonably structured. I apologize for the delay but was waiting for some type of outcome regarding the future of my facility. I am in the process of making photocopies of the replies and mailing them to those who requested a copy. Those who requested anonymity will have any information regarding their identity removed.
There is clearly a trend at many Universities to attempt some cost recovery via a charge back system for microscopy facilities. This is not the issue, in my opinion. Microscopy facilities are expensive to run and some method for defraying the costs is a fiscally sound idea. However, based on the responses that I received, no US University generates revenue strictly from users to pay ALL of the bills (this includes all salaries for ALL staff, service contracts, repairs, supplies, etc). There were 2 non-US Universities who were able to do this but admitted that they have several large industrial contracts that made this possible. One of these still struggled every year to try to generate enough revenue.
} From those who provided such information, most microscopy (EM) facilities recovered from 20-40% of operating costs from users fees. Typically, 40-55K was generated in a year in larger institutions with several workers.
Clearly, most University microscopy facilities require subsidies in order to continue operation. A number of the facilities are fortunate to at least have salaries covered by their respective University and are only responsible for recovering service/maintenance and supplies (which is still no minor task). Others are not so fortunate and preside over facilities with ever shrinking support and an ongoing battle to justify continued subsidy. Some suggested that microscopy facilities should be considered the cost of doing business and that often such facilities are not given credit for the overhead generated from successful grant funding and money from students who take microscopy courses. Total cost recovery for microscopy facilities becomes dangerous when this is the primary focus and used as the sole basis for evaluation of the facilities success. Exploratory projects that have some risk of failure are too expensive to undertake since attempts to work out the most desirable protocol may (for example, immuno-gold labeling) still prove unsuccessful even when all technical precautions are heeded.
A few asked how I went from 0 K to about 65 K in one year. It wasnt easy. First I went to all the applicable colleges and gave a presentation on contemporary techniques in microscopy and molecular cytology. This seemed to generate a lot of interest. The rates were set at $50/hr beamtime and $25/hr for time I spent training students or preparing samples. This was affordable to most and I was quite busy. 5 months later I was required to raise rates to $75/hr beamtime (with me operating the microscope) and $50/hr preparation time. In order to offer grad students a less expensive alreally "multi-user" if only a few can afford to use it. I am addressing this issue with very good success by aggressively assisting researchers in writing the microscopy portion of their grants so that they can use the facility again. This has helped a lot. In addition, within the last several months we have incorporated a confocal microscope (which has been extremely popular) and an atomic force microscope into the facility. This is very beneficial because I can assist users with the most appropriate microscopy technique for their specific project.
In any event, the responses from a few dozen other facilities proved to have a very significant impact on at least some administrators and I am hoping that others can benefit as well. Please feel free to contact me if you have not already done so and would find the compilation useful. Also, please understand that my priority is still to keep things busy here so my response may be a bit slow but I will be happy to continue the dialog offline if anyone has more specific questions.
Best Regards,
Kirk J. Czymmek, Ph.D. Biological Electron Microscopy Facility University of Delaware Newark, DE 19716 kirk-at-udel.edu
On Wed, 22 Apr 1998, Kirk J C Czymmek wrote:
} } Dear Colleagues, } } I need information to educate the administration on how close to "cost } neutrality" a microscopy facility can get. I currently supervise a } facility with a part-time employee to assist in repairs. Much of the } equipment is 20-25 years old and is constantly breaking down. I am the } sole revenue generater with a few students who have been trained to use } the microscope and have beamtime hours. Within a year or so of operation } the facility went from generating $0.00 in revenue to ~$60K maybe } 70K/year. This doesn't pay all the bills of an EM Facility with 2 TEMs and } an SEM as well. My question is, is their any facility that is COMPLETELY } cost neutral with revenue only for service work provided? Are the numbers } I describe for a medium sized University reasonable? I would greatly } appreciate any input. If their is a University that spends only what they } bring in for doing samples in a multi-user environment please contact me I } be happy to know how you did this. } } Best Regards, } } Kirk J. Czymmek, Ph.D. } Biological Electron Microscopy Facility } University of Delaware } Newark, DE 19716 } kirk-at-udel.edu } } }
Regarding a good glow discharge unit, we also use one made very similarly to that mentioned by Dick Briggs and described in the paper: Aebi and Pollard, 1987, Journal of Electron Microscopy Technique 7:29-33. It works great and can be made in a minimally equiped shop for about $200.00. You will, however, need a vacuum pump (a considerable expense if you do not have one laying around already).
Good Luck!
Doug ---------------------- Douglas R. Keene Associate Investigator Shriners Hospital Microscopy Unit Portland, Oregon 97201 DRK-at-shcc.org
Hello, I've been trying to embed brains from one day old rats perfused with 4% paraformaldehyde. The sections I've been getting have a number of cracks throughout the tissue. In particular, the cortex seems to separate from the rest of the brain. Below is a copy of the embedding protocol I am using. I would appreciate any suggestions/ modifications to the protocol which would improve the condition of the tissue. Thanks in advance-
Joanne
postfix for 16 hrs; PBS wash - 1 hr at 4 deg 0.85% Saline - 1 hr at 4 deg 1:1 saline/ 100% EtOH - 30 min at RT 70% EtOH (twice) - 30 min at RT 85% EtOH - 60 min at RT 95% EtOH - 60 min at RT 100% EtOH (twice) - 60 min at RT 100% Xylene (twice) - 60 min at RT 1:1 xylene/paraffin - 90 min at 60 deg. 100% paraffin (three times) - 20 min at 60 deg.
The objective stigmator for my Hitachi (H-300) transmission electron microscope has ohmed out and I am having problems locating another stigmator. Does anyone have an H-300 not in use that I might scavenge/purchase that part?
Thank you in advance,
Ginger
Ginger Baker EM Lab Manager OMS Secretary/Treasurer Research Dept., Oklahoma State University College of Osteopathic Medicine 1111 W. 17th St. Tulsa, OK 74107 Phone: (918) 561-8232 FAX: (918) 699-8629 http://osu.com.okstate.edu/dept/research/content/gbaker.htm lizard-at-osucom-fs02.ocom.okstate.edu
I apologize I just sent a posting regarding "Microscopy Facility Revenue Update" and while copying into my email a relevant portion was cut. For those who were interested and confused the following two lines are corrected below.
"$50/hr preparation time. In order to offer grad students a less expensive alreally "multi-user" if only a few can afford to use it. I am addressing"
SHOULD READ:
"$50/hr preparation time. In order to offer grad students a less expensive alternative they could be trained via an EM class or by me at $25/hr and then $50/hr if they operated the microscope without me. Once the rate change went into place most users could not afford to do EM. The facility was then being supported by 5 or 6 users with mega-grants and an occasional small EM project. This indicates that there is most definitely a threshold that one can charge users, and it becomes questionable whether a facility is really "multi-user" if only a few can afford to use it. I am addressing..."
Joanne: You don't indicate the size of your rat brain pieces which would make a difference in processing times. Basically I would think a longer processing time might be better 10 - 15 hours for the dehydration thru infiltration steps with an hour in each paraffin with vacuum applied in the last paraffin bath (58 degrees with polyfin) . If your tissue is brittle isopropyl alcohol would be preferable especially for the absolute alcohol steps. Butyl alcohol would harden (and shrink) even less when used in the absolute baths ( works wonders on muscle but takes longer to dehydrate and is very stinky). bob
We are in the process of purchasing an image analysis system for our department. We have narrowed our choices to the systems made by the following two companies:
-Buehler, Ltd. -Definitive Imaging
We need to decide on one of these two systems during the next week. Any comments about the pros & cons of each would be greatly appreciated. Also, I am not too familiar with the Definitive Imaging Company. Any information you can share about your experiences dealing with this company is also appreciated.
Thanks in advance, Viola L. Acoff, Ph.D. Assistant Professor The University of Alabama Department of Metallurgical & Materials Engineering Box 870202 Tuscaloosa, Alabama 35487-0202 phone (205) 348-3761 fax (205) 348-2164 Email VACOFF-at-COE.ENG.UA.EDU
I need some advice for the processing of very tiny wallaby embryos for TEM, without losing or damaging them. I have tried processing them from go to whoa in microfuge tubes with some success and also the V vials. I use an epon-araldite mix and it can be very hard to actually see the embryo when it is in the resin. I would really appreciate some help as these are the bane of my life TIA Joan Clark
In order to use a carbon coater w/o a thickness monitor and determine the thickness, it is important to have a highly polished brass or gold substrate as a calibration sample. I was interested in any information on manufacturers of these types of products or if any old polished brass item can be used. I have obtained info on the interference colors that correspond to different approximate thickness ranges, but in order to use this technique, there exists the need for a polished substrate as the calibration sample. Appreciate any information. Thanks, Steve
The Electron Microscopy Core Facility at the University of Missouri-Columbia has an open position for a full-time benefit-eligible senior electron microscopy specialist. Applicants should have a degree in Biology or a related field, and experience in the application of microscopy in the life sciences including plant, animal and microbial systems. Qualified applicants should have good communication skills to work with a variety of core facility users. These include faculty, staff, and students from a diversity of departments at the University.
Responsibilities will include sample processing, sectioning, and analysis with transmission and scanning electron microscopy, service of equipment, individual training and instruction for researchers, assistance in teaching a graduate laboratory course, and service to faculty and staff. Preference will be given to those demonstrating experience with immuno-electron microscopy. Applicants must also be able to supervise a full-time technician, and other part-time staff, coordinate general EM Core functions, such as scheduling user time, train others in the use of equipment, purchase and maintain equipment, maintain appropriate inventory of supplies, and participate in workshops to develop new skills. Basic personal computing skills are required. Salary is competitive and commensurate with experience. Position available immediately. Applications will be accepted until qualified candidate is identified. Please apply to:
Dr. Heide Schatten Associate Professor Department of Veterinary Pathobiology Director, Electron Microscopy Core Facility University of Missouri-Columbia Columbia, MO 65211 TEL: (573) 882-2396 FAX: (573) 884-5414 e-mail: vmhsch-at-showme.missouri.edu MU Electron Microscopy Core (EMC), Molecular Biology Program Web Site: http://www.missouri.edu/~emc/
Heide Schatten, Ph.D. Associate Professor Department of Veterinary Pathobiology Veterinary Medicine Building University of Missouri-Columbia 1600 E. Rollins Street Columbia, MO 65211 TEL: (573) 882-2396 FAX: (573) 884-5414 alternative e-mail: hschatte-at-facstaff.wisc.edu
Viola You seem to have already narrowed your choice to the two systems. However, in your initial survey, did you consider software by Scanalytics. They have a great image analysis program for both MAC and PC with additional support for gel analysis and 3-D. They were formally known as Signal Analytics but merged with another company last year to form the new Scanalytics. Their tech support is fantastic....I often get the founder and head of the company when I call for assistance....they all have amazing patience when helping guide the novice user through various procedures.
They can be contacted as follows:
Scanalytics 8550 Lee Highway, Suite 400 Fairfax, VA 22031 Phone: 703-208-2230 FAX: 703-208-1960 www.scanalytics.com
If you haven't made an absolutely final decision, do take a look at what they have to offer. They will have a booth at the upcoming MSA annual meeting in Atlanta.
Debby Sherman
++++++++++ Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-aux.btny.purdue.edu Purdue University or: emcenter-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057
--------------------------------------
We are in the process of purchasing an image analysis system for our department. We have narrowed our choices to the systems made by the following two companies:
-Buehler, Ltd. -Definitive Imaging
We need to decide on one of these two systems during the next week. Any comments about the pros & cons of each would be greatly appreciated. Also, I am not too familiar with the Definitive Imaging Company. Any information you can share about your experiences dealing with this company is also appreciated.
Thanks in advance, Viola L. Acoff, Ph.D. Assistant Professor The University of Alabama Department of Metallurgical & Materials Engineering Box 870202 Tuscaloosa, Alabama 35487-0202 phone (205) 348-3761 fax (205) 348-2164 Email VACOFF-at-COE.ENG.UA.EDU
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Hi, We'd like to do some cryoSEM. Does anyone in the Atlanta area (or anywhere in the southeast US) have a cryostage on their SEM?
thanks, beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
Steven asks ... } } In order to use a carbon coater w/o a thickness monitor and } determine the } thickness, it is important to have a highly polished brass or } gold substrate } as a calibration sample. I was interested in any information on } manufacturers of these types of products or if any old } polished brass item can be used. ...
I sputter Au onto coverslips to the point of opaquecity (sp?), which I would guess is 80nM ... works perfect.
... hope this helps :o)
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
First Circular for the 26th Scottish Microscopy Group Symposium
WEST PARK CONFERENCE CENTRE UNIVERSITY OF DUNDEE
Wednesday 11th November 1998
After the hugely successful 1997 =91SILVER ANNIVERSARY=92 of the=20 ScottishMicroscopy Symposium, we are now embarking for destination=20 =91GOLD=92. This years meeting will take place at the above venue and=20 the organising committee has arranged a Scientific Programme that=20 we hope will appeal to all microscopists.
The following topics will be presented by key-note speakers
Microscopy in studies of plant surface, structure and function=20 Chris Jeffree, Dept of Botany, University of Edinburgh.=20 =20 Apoptosis - when discretion is better than valour=20 David Harrison, Dept of Pathology, University of Edinburgh.=20 =20 Cathodal luminescence microscopy=20 Adrian Finch, Dept Environmental Sciences, University of Hertfordshire.=20 =20 Cryo Techniques=20 Alan Robbins, Oxford Instruments
These invited talks will be interspersed with short presentations and offer= s of these and/or posters can be made on the enclosed sheet.=20 A Second circular will be sent out in the near future containing the final programme and a registration form (registration cost =A320).
These meetings provide an opportunity to meet and share ideas with fellow= =20 microscopists. We look forward to seeing you.
For latest information visit our Web Site at http://www.abdn.ac.uk/~nhi691/= smg98.htm
Kevin Mackenzie EM Unit, Dept Zoology University Of Aberdeen Tillydrone Avenue Aberdeen AB24 2TZ Tel 01224-272847 Fax 01224-272396
} I was interested in any information on } manufacturers of these types of products or if any old polished brass item } can be used.
Anything you can polish should be OK. Try to choose something that you don't mind repeatedly cleaning. I ultimately had our shop mill me a brass cylinder that fit into our tilting-rotating stage so that it would be coated exactly as the stubs were, but anything that is at the same height and distance as the samples to be coated should do.
Aloha, Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Dear Steve, Any old piece of brass will do, freshly polished with any metal polish or diamond 5 micron. I use a one inch diameter plug, 1/4 inch thick, others I know put their samples on a piece of brass sheet. I would hate to pay EM suppliers prices for such a simple item, but I'm sure Chuck Garber would sell you one.
You wrote: } In order to use a carbon coater w/o a thickness monitor and determine the } thickness, it is important to have a highly polished brass or gold substrate } as a calibration sample. I was interested in any information on } manufacturers of these types of products or if any old polished brass item } can be used. I have obtained info on the interference colors that } correspond to different approximate thickness ranges, but in order to use } this technique, there exists the need for a polished substrate as the } calibration sample. Appreciate any information. Thanks, Steve Regards, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca
What is the best scanner for digitizing high resolution transmission electron microscopy images? Both dynamic range and resolution are important to my application. Quality is more important than price to me. I would prefer HP scanners but they don't seem to make any high-end scanners.
Ching-Hwa Kiang
========================================== Dr. Ching-Hwa Kiang Visiting Assistant Professor Cram Teacher-Scholar Department of Chemistry and Biochemistry University of California Los Angeles, CA 90095-1569 Tel: (310) 206-0563 Fax: (310) 206-4038 Email: chk-at-chem.ucla.edu ==========================================
Rick Felten 06/19/98 05:05 PM If one examines the two exposed surfaces of a failed solder joint with SEM/EDS, is there a book or journal article that correlates the findings to a specific failure mode? Thanks Ric
pchouse wrote: } } I hope you get this message. It came from } http://msa.microscopy.com/MicroscopyListserver/FAQ.html } PAM } } --------------------------------------------------------------- } } Subject: vibration isolation tables } Date: Fri, 12 Jun 1998 20:01:34 -0400 } From: "Ron L'Herault" {lherault-at-bu.edu} } To: {microscopy-at-sparc5.microscopy.com} } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } We have moved our lab (groan) and the new site is not ideal for our scanning } microscope. It looks like we need a vibration isolation table on the column } section. I'm looking for recommendations for good vendors. } } Thanks } } Ron } lherault-at-bu.edu I am unable to reach this person who sent a request appearently from your website of information . Messag received from receiving address is there is no such person. Your help would be greatly appreciated. Ted Cooper Scien-Tech Services / tcooper-at-sprintmail.com
A student at our university (Texas Christian University) working on her Ph.D. in neuroscience has been trying to stain dendrites using the Golgi-Cox technique. The primary reference she has been following is:
Kolb, Bryan and Jim McClimans, 1986, A process for cryosectioning of Golgi-Cox tissues, Stain Technology, 61, 379-380.
The main problem she has encountered is a difficulty in sectioning the tissue. Also, if and when a section is made it seems to dry out immediately upon contact with the slide.
Any suggestions or any other techniques you might know about would be most welcome. The student's name is Jocelyn Cooledge and her email address is: jjcooledge-at-delta.is.tcu.edu. Please reply directly to her.
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I will be traveling outside the US (Europe, Australia, etc.) and want to = use my PowerBook 3400 Mac Laptop to communicate with back home and for = microscopy presentations. Question: Can one use ordinary power adapters from( 110 to 220 or = whatever) on the computer line? Do standard surge protects work or since = they are made for 110, would they be underrated? If the already = mentioned alternatives do not work, what does? Would appreciate hearing from the experienced microscopist travelers out = there in cyberspace. Thanks in advance, Judy Murphy
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
Can we have some feedback on what is involved in upgrading a JEOL 2000 series 200kV TEM from Freon to SF6?
An idea of cost would be of great interest.
TIA,
Mel Dickson ***************************************************** Mel Dickson, Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
I used to work for The Open University in England and I believe that there was some research work being carried out in to the above subject. For contact names and numbers see the Open University Web site: www.open.ac.uk.
The relevant information can be found in the Technology Faculty under the Materials Engineering Department.
In our vast knowledge base does anyone have any suggestions for making long edge glass knives, i.e. ~ 25mm or so as used for histology or rotary microtomes, with an LKB knife maker or glass breaking pliers?
Obviously, ideally we'd like to use a 'Ralph Knife' maker as sold by several vendors, but the $2k price tag is prohibitive presently (sorry guys) ... unless some has one gathering dust some where they'd be willing to part with....?
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
Dear Judy, I know that when a friend of mine tried that, the power conversion was the easy part, it was the phone connections that were much more difficult to resolve. Each country seems to have a different phone system and finding the converters isn't easy. Try Fry's or Radio Shack for a phone jack conversion kit.Good luck. The power was easy, since most computers have a switch to switch from 110v. to 220 v. You just need a plug converter, not a power converter. You wrote:. } } I will be traveling outside the US (Europe, Australia, etc.) and want to use my PowerBook 3400 Mac Laptop to communicate with back home and for microscopy presentations. } Question: Can one use ordinary power adapters from( 110 to 220 or whatever) on the computer line? Do standard surge protects work or since they are made for 110, would they be underrated? If the already mentioned alternatives do not work, what does? } Would appreciate hearing from the experienced microscopist travelers out there in cyberspace. } Thanks in advance, } Judy Murphy } } } Judy Murphy } Microscopy Technology Center } San Joaquin Delta College } 5151 Pacific Ave } Stockton, CA 95207 } 209/954-5284 } FAX 209/954-5600 } e-mail; jmurphy-at-sjdccd.cc.ca.us Regards, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
There is a Hayat ref. to a paper on making Ralph knives by hand: Bennett et al., (1976) Stain Technology 51:71
I haven't tried it; don't even have the paper, but I'd like to hear from anyone who has as to how successful they were.
Tamara Howard CSHL
On Mon, 22 Jun 1998 edelmare-at-casmail.muohio.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } In our vast knowledge base does anyone have any suggestions for making long edge glass } knives, i.e. ~ 25mm or so as used for histology or rotary microtomes, with an LKB knife } maker or glass breaking pliers? } } Obviously, ideally we'd like to use a 'Ralph Knife' maker as sold by several vendors, } but the $2k price tag is prohibitive presently (sorry guys) ... unless some has one } gathering dust some where they'd be willing to part with....? } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Supervisor } 352 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } } "WE ARE MICROSOFT. } RESISTANCE IS FUTILE. } YOU WILL BE ASSIMILATED." }
I have not traveled there, but am familiar with theoretical requirements...
Depending on the circuitry and specs, some voltage converters should work. A "real" 220 to 110 (or 240 to 120) transformer should be used. Be sure to use any 120vac surge supressors on the 120 side NOT the 220 side of the transformer. ...The plugs won't match anyway...
Also, check the frequency, (unsure, but) you may be dealing with 50 Hz. rather than 60 Hz. Most modern computer switching power supplies will never know the difference, but the converting transformer should be rated accordingly. Operating a 60 Hz. (only) transformer at 50 Hz. can cause overheating.
A large transformer should not be needed. The VA rating should be about 1.4 times the computer power consumption in watts.
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I will be traveling outside the US (Europe, Australia, etc.) and want to use
my PowerBook 3400 Mac Laptop to communicate with back home and for microscopy presentations. Question: Can one use ordinary power adapters from( 110 to 220 or whatever)
on the computer line? Do standard surge protects work or since they are made for 110, would they be underrated? If the already mentioned alternatives do not work, what does? Would appreciate hearing from the experienced microscopist travelers out there in cyberspace. Thanks in advance, Judy Murphy
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
by EMAIL1.BYU.EDU (PMDF V5.1-10 #23832) with SMTP id {01IYJB413TPW98G9IP-at-EMAIL1.BYU.EDU} for Microscopy-at-sparc5.microscopy.com; Mon, 22 Jun 1998 11:51:08 MDT
We are going to be offering a new service in our lab -- that of = quantitative image analysis. We are trying to decide what would be a = reasonable charge for performing this service. Would other labs = (preferably academic) offering this type of service please contact me = with their rate schedule.
Thanks,
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Michael D. Standing BYU Microscopy Lab e-mail: MDStandi-at-bioag.byu.edu =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
First on the connector issue..... in Europe, at least where I have traveled, I use a double female jack (american configuration), and take the plug out of the phone. MOST places I have traveled seem to have the american mail connector on the phone end of the cord. Also saves one from climbing over dressers, under tables to get to the wall jack. I will not say, don't buy the International connector kit, but do also make sure to buy the double female USA style.
Now for MY problem. I will be traveling in the USA this summer.... have paper will travel (presenting at Inter/Micro 50), and I am thinking of taking my lap top with me for email home and to keep up with the local press on the web. I know the power or the phone jacks will not be a problem, but how does one get on the web with no connections to servers in the states. Once there I can use my servers on this side of the Atlantic.
Any ideas on this appreciated...
Shalom from Jerusalem, Azriel
On Mon, 22 Jun 1998, Mary Mager wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Judy, } I know that when a friend of mine tried that, the power conversion was the } easy part, it was the phone connections that were much more difficult to } resolve. Each country seems to have a different phone system and finding the } converters isn't easy. Try Fry's or Radio Shack for a phone jack conversion } kit.Good luck. The power was easy, since most computers have a switch to } switch from 110v. to 220 v. You just need a plug converter, not a power } converter. } You wrote:. } } } } I will be traveling outside the US (Europe, Australia, etc.) and want to } use my PowerBook 3400 Mac Laptop to communicate with back home and for } microscopy presentations. } } Question: Can one use ordinary power adapters from( 110 to 220 or } whatever) on the computer line? Do standard surge protects work or since } they are made for 110, would they be underrated? If the already mentioned } alternatives donot work, what does? } } Would appreciate hearing from the experienced microscopist travelers out } there in cyberspace. } } Thanks in advance, } } Judy Murphy } } } } } } Judy Murphy } } Microscopy Technology Center } } San Joaquin Delta College } } 5151 Pacific Ave } } Stockton, CA 95207 } } 209/954-5284 } } FAX 209/954-5600 } } e-mail; jmurphy-at-sjdccd.cc.ca.us } Regards, } Mary } Mary Mager } Electron Microscopist } Metals and Materials Engineering } University of British Columbia } 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } CANADA } tel: 604-822-5648 } fax: 604-822-3619 } e-mail: mager-at-interchg.ubc.ca }
Dear Azriel, I'm afraid you are out of my league. Access to your e-mail is a problem that you can best discuss with your Internet Service Provider or the computer genius at your university. You wrote: } This is almost a reversal of Judy's message. } } First on the connector issue..... in Europe, at least where I have } traveled, I use a double female jack (american configuration), and take } the plug out of the phone. MOST places I have traveled seem to have the } american mail connector on the phone end of the cord. Also saves one from } climbing over dressers, under tables to get to the wall jack. I will not } say, don't buy the International connector kit, but do also make sure to } buy the double female USA style. } } Now for MY problem. I will be traveling in the USA this summer.... have } paper will travel (presenting at Inter/Micro 50), and I am thinking of } taking my lap top with me for email home and to keep up with the local } press on the web. I know the power or the phone jacks will not be a } problem, but how does one get on the web with no connections to servers in } the states. Once there I can use my servers on this side of the Atlantic. } } Any ideas on this appreciated... } } Shalom from Jerusalem, } Azriel Best of Luck, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
Eunsung Park wrote: ================================================= Does anyone know about the conductive epoxy that can stand high T (~500C)? Thank you. ================================================= I have never heard of a conductive epoxy that would withstand that high of a temperature. However, sometimes our SPI Silver Paste Plus™, which comes in a metal squeeze tube, is mistakenly described as being an epoxy. Its main use, at such high temperatures is to "glue" a silicon water to a heater head for those doing thin film coatings research.
Full information is on our website.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
} ---------- } From: Tamara Howard[SMTP:howard-at-cshl.org] } Sent: June 22, 1998 11:39 AM } To: edelmare-at-casmail.muohio.edu } Cc: microscopy-at-Sparc5.Microscopy.Com } Subject: Re: Histology Knives / Ralph Knife Maker ? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} Now for MY problem. I will be traveling in the USA this summer.... have } paper will travel (presenting at Inter/Micro 50), and I am thinking of } taking my lap top with me for email home and to keep up with the local } press on the web. I know the power or the phone jacks will not be a } problem, but how does one get on the web with no connections to servers in } the states. Once there I can use my servers on this side of the Atlantic.
The answer is to use a terminal at a library/university or subscribe to a US ISP for the duration of your stay. No long term obligation is necessary. (This is not a plug for ATT World-Net but I use them throughout the US when I travel although I use my university ISP at home.)
Over the past several years we have been asked many times, particularly by researchers new to corrosion casting, which color Mercox they should use, red, blue or clear. Since they are all the same except for the color, it appears the choice should be a based on personal preference and application. If anyone has any thoughts on which color should be used when, please respond and we will pass the information on to Fred Hossler so he can present it at his vascular corrosion casting sympossuim at MSA'98 in Atlanta on July 13th.
Thanks in advance,
John Arnott Chairman
LADD RESEARCH 13 Dorset Lane Williston, VT 05495
TEL 1-800-451-3406 (US) 1-802-878-6711 (FROM ANYWHERE) fAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.msa.microscopy.com/SM/LADD
We're not into Macs in this department, but visitors from US and Japan usually don't have any problems with our 230V 50 Hz supply as the Mac plugpacks (those that I've seen, at least) will work on anything from 100V to 250V (darned clever, these people). Check in your manual. Australia and UK are 240V 50Hz.
Ritchie
} I will be traveling outside the US (Europe, Australia, etc.) and want to use my PowerBook 3400 Mac Laptop to communicate with back home and for microscopy presentations. } Question: Can one use ordinary power adapters from( 110 to 220 or whatever) on the computer line? Do standard surge protects work or since they are made for 110, would they be underrated? If the } lready mentioned alternatives do not work, what does? } Would appreciate hearing from the experienced microscopist travelers out there in cyberspace. } Thanks in advance, } Judy Murphy }
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Has anyone used Image Space Software for looking at dual channel "single section" images. Specifically using the dual channel overlays with two colours. I may be missing something but I cannot get the dual or stereo applications to work with single images. Probably this is not possible with this software it seems to be looking for depth information.
Also which antifade agent do people recommend for fluorescent antibody probes for use with confocal.
} There is a Hayat ref. to a paper on making Ralph knives by hand: Bennett } et al., (1976) Stain Technology 51:71 } } I haven't tried it; don't even have the paper, but I'd like to hear from } anyone who has as to how successful they were. } } Tamara Howard
It works rather well. You score on your "standard" glass knifemaker, and break with hand pressure. Worth trying.
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/
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Mel, We recently switched from Freon to SF6. You need to get a new gun assembly, to run at a higher pressure (3Kg instead of 2), and an exchange tank (rebuilt and tested in Boston -- since the changes inside are fairly extensive). Takes 3 to 5 days and costs about $28K here in the United States. -Mike O'Keefe ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } Hello world, } } } } Can we have some feedback on what is involved in upgrading a JEOL 2000 } } series 200kV TEM from Freon to SF6? } } } } An idea of cost would be of great interest. } } } } TIA, } } } } Mel Dickson } } ***************************************************** } } Mel Dickson, } } Director. } } Electron Microscope Unit, } } University of New South Wales. } } Sydney NSW 2052 Australia } } } } Phone (+612) 9385-6383 } } Fax (+612) 9385-6400 } } Website {http://srv.emunit.unsw.edu.au} } } ***************************************************** --------------4B8531A61E8BCF780EED5966 Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for O'Keefe, Michael A. Content-Disposition: attachment; filename="vcard.vcf"
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Dear Azriel, Check with your ISP. They may be a GRIC partner (see http://www.gric.com/). If so you can access your email account from anywhere in the world using a local phone call.
Yours sincerely,
Paul Thomson Techncial Director Thomson Scientific Instruments Web page: http://werple.net.au/~tsi/ Fax: +613 9663 3680 Tel: +613 9663 2738
First Announcement S4G International Conference on Stereology, Spatial Statistics and Stochastic Geometry Prague, Czech Republic, 21 June - 25 June 1999
under the auspices of Academy of Sciences of the Czech Republic Czech Technical University Charles University Czech Society for Cybernetics and Informatics
International Advisory Committee: J. Cwajna ( Poland ) D. Jeulin ( France ) L. Kubinova ( Czech Republic ) B. Pakkenberg ( Denmark ) D. Stoyan ( Germany )
S4G'99 Next in the series of conferences on Stereology and related sciences held in the Czech Republic and in the Slovak Republic (CMMSIA'82, CASIA'88, 6ECS'93).
Scope: This conference will be a meeting of scientists interested in spatial data evaluation, stochastic modelling and geome1rical sampling. Participation of people from applied fields is strongly desired, including quantitative microscopy and image processing.
Main topics of the conference are: Application of Stereology and image analysis in life sciences and material sciences. Application of spatial statistics in geostatistics, environmental and other sciences. Stochastic geometry and random sets. Integral geometry and theoretical Stereology. Quantitative image analysis and mathematical morphology.
Program: The scientific program will consist of keynote lectures, oral and poster presentations. No parallel sessions are envisaged The proceedings of contributed papers will be published
Venue: All scientific activities will be hosted by the Institute of Physiology, Academy of Sciences of the Czech Republic, located in the southern part of Prague with public transport connection to the center of the city. Accommodation in hotels and student hostels in Prague will be offered and cultural events for participants and accompanying persons will be organized.
Registration and important date:: If you are interested, please, return the completed reply card to the secretariat fill in the registration form on the conference WWW page. Your camera-ready abstract not exceeding 200 words on A4 sheet with wide margins should be sent to the same address not later than November 15, 1998. Alternatively you can send MS Word 6 file (with True Type fonts included) as an attachment to E-mail. Then you will receive the second announcement till the end of December. The papers for the proceedings should be submitted by February 15, 1999. Final program will be distributed during May.
Adjoining Course: Serology in Plant Anatomy 27 June - 31 June 1999
(contact: albrecht-at-prfdec.natur.cuni.cz or kubinova-at-biomed.cas.cz)
Local Organising committee: Viktor Benes, chairman Jana Albrechtova Vratislav Horalek Jiri Janacek Marie Jirkovska Lucie Kubinova Jan Rataj Ivan Saxl
Conference Secretariat: Jiri Janacek Institute of Physiology Videnska 1083 142 00 Praha Czech Republic Fax: (4202) 44472269 Phone: (4202) 4752768 E-mail: janacek-at-biomed.cas.cz WWW page: http://www.biomed.cas.cz/s4g.html
The most Important dates:
Abstract: November 15, 1998
Papers: February 15, 1999
Jiri Janacek Institute of Physiology Videnska 1083 142 20 Praha Czech Republic
Registration form:
Name:
Inst/Dept:
Address:
Phone:
Fax:
E-mail:
Title of my oral/poster presentation:
Signature:
======================================================================== Krzysztof Jan Huebner
{hubner-at-IOd.krakow.pl} :-)
FOUNDRY RESEARCH INSTITUTE Research Materials Department Manager Structural and Physical Research Laboratory str. Zakopianska 73 Call (*48 12) 2665022 ext.356 30-418 KRAKOW - POLAND Fax (+48 12) 2660870
Next year, 1999, it will be 50 years since Philips sold its first transmission electron microscope, the EM100. In view of this special anniversary, we are trying to locate the oldest working model of this type. If you have information that can assist us, please let us know.
Your help is very much appreciated.
Bert Heijligers
FEI / Philips Electron Optics Bldg. AAE PO Box 218 5600 MD Eindhoven The Netherlands Tel: +31 40 2766225 Fax: +31 40 2766587 Email: bjh-at-eo.ie.philips.nl {mailto:bjh-at-eo.ie.philips.nl} Website: www.feic.com
Does anyone out there have experience of conditions for cleaning(mostly)cross sectional semi/super conductor specimens with a South Bay plasma cleaner?
We are setting up a new Electron Microscopy lab and have a plasma cleaner. Up to now success has been hit or miss, I would like to hear comments from other labs that have purchased such equipment. Published data on this technique seem to use a much lower bias voltage than we can achieve at 10W, although the manufacturer has explained this as a component error in the early versions.
Regards
Alan W Nicholls, PhD
NB NEW PHONE NUMBERS
Mailing Address:- University of Illinois at Chicago Research Resources Center (MC 937) 835 South Wolcott Avenue Chicago IL 60612-7341 Fax: 312 996 0539
Location Address:- Room 110, Research Resources Center - East 845 West Taylor Street Tel: 312 996 1227
I used & made this type of Ralf Knife around 20 years ago.
1. Start off with a 3 X 1 inch slide and diamond score using a small set square at the desired position.
2. Place a wood toothpick on the opposite side of your score line offset from the line by say 2mm, this distance is critical and may need to be made larger or smaller depending on the profile you achieve. Take care and wear leather gloves and protective goggles, carefully press down on the slide and you should have your Ralf Knife.
3. To use the knife on a microtome, I placed a standard steel knife in the holder with a piece of sheet metal under the steel knife to act as a ledge to support the Ralf Knife. Make your Ralf Knife a few mm. longer than the distance from the edge of the knife to the ledge at the base of the knife.
4. To fix the Ralf Knife onto the front face of the knife, paraffin wax was used by slightly heating both knife and Ralf Knife, and then waiting to both cooled down.
I hope this information is of assistance to you, please say if you need anymore information.
Best Regards.
Alan Bright
Bright Instrument Co. Ltd. St Margarets Way Huntingdon PE18 6EB England
---------- } From: edelmare-at-casmail.muohio.edu } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Histology Knives / Ralph Knife Maker ? } Date: Monday, June 22, 1998 04:29 } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } In our vast knowledge base does anyone have any suggestions for making long edge glass } knives, i.e. ~ 25mm or so as used for histology or rotary microtomes, with an LKB knife } maker or glass breaking pliers? } } Obviously, ideally we'd like to use a 'Ralph Knife' maker as sold by several vendors, } but the $2k price tag is prohibitive presently (sorry guys) ... unless some has one } gathering dust some where they'd be willing to part with....? } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Supervisor } 352 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } } "WE ARE MICROSOFT. } RESISTANCE IS FUTILE. } YOU WILL BE ASSIMILATED."
"Analytical Electron Microscopy of Oxynitride Glass Ceramics"
A post-doctoral position is available in the Division for Microscopy= and Microanalysis at Chalmers University of Technology within the framework= of the Training and Mobility of Researchers (TMR) Programme. Qualified candidates should have a Ph.D. in materials science, physics or chemistry, and a documented background in transmission electron microscopy.= It will be considered an advantage if he or she has some experimental experience= of any of the following techniques: energy dispersive X-ray spectroscopy (EDX), electron energy loss spectroscopy (EELS), convergent beam= electron diffraction (CBED) or high resolution lattice imaging. The applicant= must be a national of a Member State of the European Community. The starting date of the appointment may be discussed, and the appointment may= last up to 16 months.
The position is funded by the TMR networks research project "Structure= and Properties of New M-Si-Al-O-N Oxynitride Glass Ceramics (M=3DY,Ln)"= which is a collaboration between seven partners in the United Kingdom, Ireland, =46rance, Sweden and Belgium. The collaboration within the established network will give the post-doctoral fellow good contacts with a number= of research groups in Europe and a good knowledge of a variety of preparative / analytical / property measurement techniques relevant to the evaluation of new ceramic materials as well as other materials outside the field= of ceramics.
The aim of the research project is to determine crystal structures= and properties of currently uncharacterised new glass-ceramic phases= in yttrium and rare earth sialon systems. These materials are potential refractory grain boundary phases in Si3N4-based ceramics, and have an interest= also as refractory surface coatings (glazes) and as interface materials in nitride-oxide and nitride-metal joints.
The analytical transmission electron microscopy work carried out= at Chalmers will focus on the chemistry and structure of rare earth= sialon glasses and their crystallisation products, and on microstructural development during nucleation and growth processes. Microanalytical= work on glass ceramics subjected to creep testing and oxidation / corrosion experiments may also be carried out.
The major part of the microscopy work will be carried out on a Philips CM200 Supertwin transmission electron microscope (TEM) with a field emission gun (FEG) and surrounding interactive instrumentation. = The FEG TEM is equipped with the Gatan imaging filter (GIF) which produces= energy filtered electron images and diffraction patterns as well as electron energy loss spectra, a Gatan off-axis CCD camera for high resolution imaging and a Link Isis energy dispersive X-ray (EDX) system for qualitative and quantitative elemental analysis including the light elements. There are also other electron microscopes in the laboratory, e.g. a Jeol 2000-FX TEM/STEM/SEM and a CamScan SEM.
Applications should be sent to: Associate Professor Lena Falk, Department of Experimental Physics,= Chalmers University of Technology, SE-412 96 G=F6teborg, Sweden e-mail: lklfalk-at-fy.chalmers.se; fax: +46 31 772 3224; phone: +46= 31 772 3321
We recently had such an upgrade done for a 2000ES by JEOL USA. The cost was between $25K and $35K, You would have to get a price from JEOL in Australia or Japan. We have not experienced any gun instabilities since. Both the gun and HV tank were replaced, and the upgrade took less than a week.
David Gelles Structural Materials Research Battelle Pacific Northwest National Laboratory Richland, WA USA Tel: 509 376-3141 Fax 509 376-0418 E-mail: ds_gelles-at-pnl.gov
-----Original Message----- From: Vetrano, John S Sent: Monday, June 22, 1998 9:05 AM To: Gelles, David S Subject: FW: SF6 upgrades for JEOL TEMs
Dave,
I figured you could answer this question if you get the chance. His address is: M.Dickson-at-unsw.edu.au
Thanks, JSV ---------- From: Melvyn Dickson Sent: Sunday, June 21, 1998 4:59 PM To: Microscopy-at-Sparc5.Microscopy.Com Subject: SF6 upgrades for JEOL TEMs
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
Can we have some feedback on what is involved in upgrading a JEOL 2000 series 200kV TEM from Freon to SF6?
An idea of cost would be of great interest.
TIA,
Mel Dickson ***************************************************** Mel Dickson, Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} 3. To use the knife on a microtome, I placed a standard steel knife in the } holder with a piece of sheet metal under the steel knife to act as a ledge } to support the Ralf Knife. Make your Ralf Knife a few mm. longer than the } distance from the edge of the knife to the ledge at the base of the knife. } } 4. To fix the Ralf Knife onto the front face of the knife, paraffin wax } was used by slightly heating both knife and Ralf Knife, and then waiting to } both cooled down. } } I hope this information is of assistance to you, please say if you need } anymore information. } } } Best Regards. } } Alan Bright } } Bright Instrument Co. Ltd. } St Margarets Way } Huntingdon } PE18 6EB } England } } e-mail: Bright-at-dial.pipex.com } Tel: +44 (0) 1480 454528 } Fax:+44 (0) 1480 456031 } } ---------- } } From: edelmare-at-casmail.muohio.edu } } To: microscopy-at-Sparc5.Microscopy.Com } } Subject: Histology Knives / Ralph Knife Maker ? } } Date: Monday, June 22, 1998 04:29 } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } In our vast knowledge base does anyone have any suggestions for making } long edge glass } } knives, i.e. ~ 25mm or so as used for histology or rotary microtomes, } with an LKB knife } } maker or glass breaking pliers? } } } } Obviously, ideally we'd like to use a 'Ralph Knife' maker as sold by } several vendors, } } but the $2k price tag is prohibitive presently (sorry guys) ... unless } some has one } } gathering dust some where they'd be willing to part with....? } } } } } } Richard E. Edelmann, Ph.D. } } Electron Microscopy Facility Supervisor } } 352 Pearson Hall } } Miami University, Oxford, OH 45056 } } Ph: 513.529.5712 Fax: 513.529.4243 } } E-mail: edelmare-at-muohio.edu } } } } "WE ARE MICROSOFT. } } RESISTANCE IS FUTILE. } } YOU WILL BE ASSIMILATED."
Margaret Springett e-mail hukee.margaret-at-mayo.edu IEM Specialist at Mayo Foundation 1426 Guggenheim Rochester, Mn. 55905
Authenticated sender is {3qt3gt-at-worldnet.att.net}
I have a client who is looking for a SEM, with EDS, that can accommodate a cable sample approximately 2 feet long without cutting it as it is involved in litigation.
Does anyone have such an instrument (and operator) for hire?
Dear Alan, } } I have a client who is looking for a SEM, with EDS, that can accommodate a } cable sample approximately 2 feet long without cutting it as it is involved } in litigation. } } Does anyone have such an instrument (and operator) for hire? } Does your client need images, or just local compositions? In the latter case, perhaps proton-induced x-ray emission (PIXE) or x-ray fluor- escence (XRF) would be suitable. I know of facilities which have done PIXE on a Gutenberg Bible, so they should be able to accomodate the size of the sample. Good luck. Yours, Bill Tivol
Marian College in Fond du Lac, Wisconsin is looking for a good used TEM for biological imaging to replace their early-60's RCA EMU3G. The closer the better, the more robust the better, and the cheaper the better. Anybody have an old scope to unload?
Bob
Dr. Robert R. Wise Department of Biology and Microbiology University of Wisconsin-Oshkosh Oshkosh, WI 54901
(920) 424-3404 tel (920) 424-1101 fax wise-at-uwosh.edu www.uwosh.edu/departments/biology/wise/wise.html
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I have a client who is looking for a SEM, with EDS, that can accommodate a } cable sample approximately 2 feet long without cutting it as it is involved } in litigation. } } Does anyone have such an instrument (and operator) for hire? } } If so, I will pass your name and number along. } } Thanks. } } Alan Stone } ASTON
A JEOL IC848 can easily be modified to accomodate a sample that large depending upon the sample thickness. email me if you need more info.
A few thoughts that come to mind: 1) Check with Amray for info on large chambers. They have built systems that can accomodate aircraft turbine blades. 2) If the cable is part of a failure investigation, then it may be worth the expense of X-raying the entire cable section. Non-destructive analysis can be done in real time and can be video taped. 3) Since failures begin at microscopic site(s), the root cause(s) may not be revealed until the cable has been dismantled appropriately. 4) Who let the lawyers in to the lab?!
Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 (978) 750-1717 crossman-at-osi.sylvania.com
What is a good software to use for SEM digital image analysis?
Thanks Kalpana
************************************************************************************ Kalpana S Katti, Ph.D. Department of Polymers and Coatings North Dakota State University Fargo, ND 58105 ph:(701)231-8410 fax:(701)231-8439 email:kkatti-at-prairie.nodak.edu ************************************************************************************
Question: I have acquired an old microscope at a flea market and would like to find out what it is. It has one eyepiece and two objectives. There are quartz crystals in the optical paths. It has a rectangular box shape with a front sliding cover to access a ceramic slabe work base. Looks like it could be a binocular disecting microscope or a comparator microscope. Having acquired it in western Kentucky it may be related to analysis of coal.
A plate on the back has: Bausch & Lomb Optical Co. Rochester, NY No. 6222 Pat. Appl'd I estimate is is from about the 1920s. My wife estimates it is from late 1800s. My goal is to get the patient information so I can replace the missing objective lenses and use it. Bausch & Lomb have not been helpful. The old patient books are not much use without knowing the year.
Email: BKurnett-at-tech.puhsd.k12.ca.us Name: Bill Kurnett (High School Teacher)
School: Del Oro High School
State: California
Zip: 95650
Question: Is there anyone who can lend a small capacity mechanical vacuum pump to Del Oro High School in Loomis, CA. The pump is needed for a low-tech beam tube which can be used to demonstrate focusing by a magnetic lens. Student Joseph Navarro and Andy Hill are working on this project under the direction of teacher Bill Kurnett.
M.E. Taylor Engineering, Inc. would like to announce its new web site to = the=0Amicroscopy community.
We can be found at:
www.semsupplies.com
Please take a browse and see how we may be of service or help to you and = your=0Acompany.
M.E. Taylor Engineering, Inc. 21604 Gentry Lane Brookeville, MD 20833 Phone: 301-774-6246 =95 FAX: 301-774-6711 =95 e-mail: Metengr-at-aol.com = =95 See us=0Aon the web at www.semsupplies.com
Dear Alan, We have a large chamber (14"x14"x8") SEM with light element EDS detector, variable pressure attachment, and digital data storage. We provide analytical services for industry and and legal community. Igor Ivanov RJ Lee Group SEM/EDS,TEM,AES,XPS,SIMS,GCMS,FTIR,ICP,AFM,XRD,EPMA Analytical Services 530 McCormick Str. San Leandro, CA 94577 510-567-0480 510-567-0488 FAX
On 06/23/98 15:48:14 you wrote: } I have a client who is looking for a SEM, with EDS, that can accommodate a } cable sample approximately 2 feet long without cutting it as it is involved } in litigation. } } Does anyone have such an instrument (and operator) for hire? } } If so, I will pass your name and number along. } Thanks. } Alan Stone } ASTON
Dear Microscopy Listserver, Please change my address from : cshannon-at-fda.net to: cshannon-at-nctimes.net Thank you for your attention. Sincerely, C Shannon
Does anybody have a new or tried and true suggestion for a TEM magnification calibration standard for a mag of 30KX using a CCD camera? I would like to have better than +/- 3% accuracy. Have tried catalase--not happy with it. Latex spheres seem to shrink about 20% (according to a 1968 paper). Diffraction gratings not acceptable--only two to three lines in the image.
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
I'm required to inject some sheep at 2 hourly intervals with Brdu. Most papers refer to a "magic" figure of 5mg/kg body weight, so per injection I'll need 250mg of Brdu. We seem to have a few problems dissolving this quantity of Brdu in a reasonable amount of saline (ie 20mls or less, in 20mls concentration would be 4mol/l). . Any thoughts on improving solubility would be appreciated.
Regards Peter Smith AgResearch Wallaceville Upper Hutt New Zealand
One quick idea would be us use something like 10nm colloidal gold particles and calibrate an image at high magnification using the particle lattice spacings, then make a (nominal) 30kX image and measure bulk features to get the actual mag at 30kX. You might want to track the magnification at one or more steps down the scale as you approach 30kX, to get better accuracy in the measurement.
There may also be some layered semiconductor samples that have highly accurate and known layer spacings that can be imaged nicely at 30kX. It seems to me that I have seen a paper or two on this type of sample, so a literature search might pop up with something (if the listserver doesn't...).
Larry
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Dr. Lawrence F. Allard Senior Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
John Wheatley wrote: ============================================ Does anybody have a new or tried and true suggestion for a TEM magnification calibration standard for a mag of 30KX using a CCD camera? I would like to have better than +/- 3% accuracy. Have tried catalase--not happy with it. Latex spheres seem to shrink about 20% (according to a 1968 paper). Diffraction gratings not acceptable--only two to three lines in the image. ============================================== If you are looking for something beyond the possibilities mentioned, we have had some good reports from persons using the newly introducted MAG*I*CAL(TM) Calibration Sample. You can get a good sense of what it looks like at magnification at URL http://www.2spi.com/catalog/standards/magical.html
It is a bit more expensive that most other "calibration" samples but it is quite good and seems to be sufficiently robust that it does not fall apart after being used a few times. And it certainly is not going to be effected by the beam.
The product is available from SPI as well as several of the other main suppliers of consumables and accessories to microscopy laboratories.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
We are looking for a low cost solution to replace an EDS analysis system on a JEOL 840 SEM. The new system should make use of our windowless Link 5474 detector, should be PC based (running Windows NT), and have the option for digital imaging.
Please reply directly to this address and not to the listserver. Hasso Weiland Alcoa Technical Center Alcoa Center, PA 15069
John, I would argue that using a grating might still work. That is, provided you get some feature that is recognizable at a lower mag, say 10K. The larger number of lines at the low mag will give you a statistically valid number for that mag. Then the size difference between that low mag and the higher one gives you the factor to calculate the higher mag. We've done it on our scopes in just this way for quite a while and it's quite accurate. Of course, with the CCD camera you will still need to know exactly what the size of your CCD pixels is. We ran into the same problem with our CCD camera (Gatan 679) (Sherman et al, Micron 1996, 27:129-139).
Hope this helps,
Jaap
-- Jaap Brink, Ph.D. Biochemistry, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030 Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu URL : http://ncmi.bioch.bcm.tmc.edu/~brink
On Wed, 24 Jun 1998, John C. Wheatley wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Does anybody have a new or tried and true suggestion for a TEM } magnification calibration standard for a mag of 30KX using a CCD camera? I } would like to have better than +/- 3% accuracy. Have tried catalase--not } happy with it. Latex spheres seem to shrink about 20% (according to a 1968 } paper). Diffraction gratings not acceptable--only two to three lines in } the image. } } John C. Wheatley } Lab Manager } Arizona State University } Center for Solid State Science } PSA-213 } BOX 871704 } Tempe, AZ 85287-1704 } } } Phone: (602) 965-3831 } FAX: (602) 965-9004 } John.Wheatley-at-ASU.Edu } } }
Does any one have an old LKB grid stainer that can be used for parts. We would like to get one for a spare. Please let me know, Tom Baginski, USUHS, Bethesda, MD
I have used the Mag-I-Cal sample that John McCaffrey and crew came up with and which South Bay Technology distributes. This sample covers the full range of Mags available in the TEM. It is done very quickly and simply and it can be used to measure the rotation calibration of the diffraction pattern and the camera constant as well. I have even used it in a High resolution SEM but its use there isn't quite as easy.
Incidentally, they have the Guiness World Record for the World's smallest ruler.
-Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Does anybody have a new or tried and true suggestion for a TEM magnification calibration standard for a mag of 30KX using a CCD camera? I would like to have better than +/- 3% accuracy. Have tried catalase--not happy with it. Latex spheres seem to shrink about 20% (according to a 1968 paper). Diffraction gratings not acceptable--only two to three lines in the image.
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
A colleague of mine is using araldite to mount samples and then performing experiments in cyclohexane. We would appreciate any information or experience of the solubility of araldite in this medium (or any others).
Thanks in advance
Dr. Giles Sanders Laboratory of Biophysics & Surface Analysis School of Pharmaceutical Sciences Nottingham University
We are looking at buying a Philips 400. Does anyone out there have any specimen holders to fit it that they would be willing to part with? Especially, does anyone have a straining stage they would part with (please, please, please!)? Double-tilt and/or low-background holders would also be of great interest. If so, please e-mail me here today or tomorrow, or, next week, e-mail me care of inal-at-nmt.edu since I will be away on travel for a while, but the people here will be able to contact me.
Thanks in advance for your time and attention.
Gill Bond Dept Materials & Met. Eng. New Mexico Tech
One last reminder - next Wednesday, July 1, is the date of the Biologic= al Sciences Meeting of the Midwest Microscopy and Microanalysis Society. = The meeting will be held at the Blood Research Institute in Milwaukee. Jim=
DiOrio, Biological Sciences Director of MMMS, has put together a great program, including a workshop on digital imaging. Tables will be avail= able for vendor literature, and there will be poster boards for any one wish= ing to display a poster.
If you intend to eat lunch with us, please remember to return your me= nu selections by tomorrow (Friday, June 26) on the forms mailed to you.
For more information, you may contact me via E-mail at jane.a.fagerland-at-abbott.com.
I'm looking forward to seeing you in Milwaukee next Wednesday!
MICROSCOPY SOCIETY OF AMERICA Sustaining Members Breakfast Monday, July 13, 1998 at 8:00 am to 9:30 am Georgia World Congress Center Room 255 W
Invited Speakers
Ralph Albrecht, MSA President Kathleen B. Alexander, 1998 Program Chair Janet Woodward, 1998 LAC Chair Charlie Meshul, 1999 LAC Chair Stuart McKernan, MSA Bulletin Brian Skepton, Springer-Verlag Philip Lesser, MSA Business Office Mary Beth Rebedeau, The Rebedeau Group
The speakers will present brief updates on important current and future Society Activities.
The favor of a reply is required.
Please R.S.V.P. to Paul Fischione, Chair - Sustaining Members Committee as soon as possible at e-mail: Paul.Fischione-at-internetmci.com or by fax: 724-325-5443
Does anyone know the correct pH for Richardsons stain used for = semithins. We are trying to match slides of skin done somewhere else in = which the ground substance (collagen, etc) of the dermis stained a purplish red.=20 TIA Hank Adams Cell Biology Integrated Microscopy Core Baylor College of Medicine One Baylor Plaza Houston, Tx 77030
Hi everybody, =20 we want to buy a scanner which can be used for both 35 mm and 70 mm EM= =20 film as well as 81x100 mm plates, probably with different adapters (?).= =20 Software must be compatible with Windows 95/NT 4.0 (e.g Adobe,=20 Freehand...). The higher resolution the better (at least 1200 dpi), th= e=20 cheaper the better, as usual. Any helpful comments or experience? -=20 would be highly appreciated. Thanks a lot, =20 Matthias
The Naval Research Laboratory is conducting a continuing search for a Director/Manager of the Marine Geosciences Division's Scanning Transmission Electron Microscopy (STEM) research facility. An announcement for this position is provided below. This opening is only open to US citizens.
GENERAL: The Marine Geosciences Division, Naval Research Laboratory (NRL), Stennis Space Center, MS, is seeking to fill the position of Director/Manager of the division's STEM research facility. The successful candidate will be an accomplished transmission electron microscopist with a Ph.D. degree in a closely related academic field, or possibly Ph.D. equivalent.
FACILITY MANAGEMENT AND OPERATION: The successful candidate will serve as the director and manager of the facility and will be fully responsible for: (1) competent, efficient, economical, and safe operation and maintenance of NRL's 300 kV STEM with Environmental Cell and supporting instrumentation for imaging and analyses including Scanning Mode Microscopy (STEM), Energy Dispersive X-ray Spectrometry (EDS), Electron Energy Loss Spectrometry (EELS), a slow scan, charge-coupled device (CCD) camera and other data acquisition systems, and a 100 kV Transmission Electron Microscope with Environmental Cell; (2) staffing the facility with high quality support personnel; (3) setting up operating procedures and protocols for all aspects of work in the facility; (4) supervision and training of technical personnel; (5) setting up the financial operations of the facility; (6) setting up a cataloging/archiving system for samples, images, etc.; and (7) ensuring that operations conform to all NRL/Navy health and safety regulations and standards.
RESEARCH: As the senior electron microscopy expert, the incumbent will conduct forefront basic and applied research directed towards developing state-of-the-art microscopy techniques and applying these to investigations of complex problems in marine geomaterials, geochemistry, microbiology, environmental processes, and related areas. A primary focus will be directed towards Naval problems particularly as involve understanding fundamental processes associated with the formation, deposition, and alteration of cohesive marine sediments and the impact on the geoacoustic and geotechnical properties of the sediments and behavior of these sediments in the marine environment. The inclusion of the environmental cell in the STEM provides NRL with one of the unique capabilities in the world. With the STEM and Environmental Cell the incumbent will be able to conduct forefront research on gaseous and aqueous chemical processes at the molecular scale.
FOR FURTHER INFORMATION: Contact Philip J. Valent, Code 7401, Naval Research Laboratory, Stennis Space Center, MS 39529-5004, telephone: 228-688-4650, facsimile: 228-688-4093, or e-mail: phil.valent-at-nrlssc.navy.mil.
_____________________________________________________________ Richard W. Fonda Naval Research Laboratory (202) 767-2622 Code 6324 (202) 767-2623 fax Washington DC 20375 _____________________________________________________________
The chemical resistance (as well as temp resistance & strength) of epoxies, for a given resin, depends greatly on the curing agent and cure conditions used with the resin. I suggest consulting Ciba literature (e.g., http://www.gluguru.com/Tech_Menu/Ciba_Araldite_Table/ciba_araldite_table .htm)or technical reps to see if the resin/curing agent your colleague is using meets their needs.
-----Original Message----- From: Giles Sanders [SMTP:Giles.Sanders-at-nottingham.ac.uk] Sent: Thursday, June 25, 1998 1:04 PM To: Microscopy-at-sparc5.microscopy.com Subject: Araldite contimination
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
A colleague of mine is using araldite to mount samples and then performing experiments in cyclohexane. We would appreciate any information or experience of the solubility of araldite in this medium (or any others).
Thanks in advance
Dr. Giles Sanders Laboratory of Biophysics & Surface Analysis School of Pharmaceutical Sciences Nottingham University
Does anyone know if this John McCaffery calibration standard is NIST tracable or if it has been NIST tested. Russ
-----Original Message-----
I have used the Mag-I-Cal sample that John McCaffrey and crew came up with and which South Bay Technology distributes. This sample covers the full range of Mags available in the TEM. It is done very quickly and simply and it can be used to measure the rotation calibration of the diffraction pattern and the camera constant as well. I have even used it in a High resolution SEM but its use there isn't quite as easy.
Incidentally, they have the Guiness World Record for the World's smallest ruler.
-Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Does anybody have a new or tried and true suggestion for a TEM magnification calibration standard for a mag of 30KX using a CCD camera? I would like to have better than +/- 3% accuracy. Have tried catalase--not happy with it. Latex spheres seem to shrink about 20% (according to a 1968 paper). Diffraction gratings not acceptable--only two to three lines in the image.
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
At 10:05 AM 6/26/98 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20 (?).=20 } Software must be compatible with Windows 95/NT 4.0 (e.g Adobe,=20 } Freehand...). The higher resolution the better (at least 1200 dpi),= the=20 } cheaper the better, as usual. Any helpful comments or experience? -=20 } would be highly appreciated. Thanks a lot, } =20 } Matthias ******************* If any single piece of hardware would fit your needs, I would think that it would be a top end, high $$$, high resolution flatbed scanner. However you MAY have difficulty finding a single piece of equipment that can do a really good job on such a wide range of film sizes. I am getting good results scanning 3=BC" x 4" EM negatives on an Agfa SnapScan 300/600 flatbed scanner. And I am sure that it would do a good job on your larger film sizes. However, although I have not tried it yet, I doubt that the results from scanning 35mm format on a large flatbed like that would be satisfactory. On the other hand the Polaroid SprintScan 35 does an excellent job on the smaller 35mm format. So we have both machines. Yeah, I know....$$$$$! Joiner Cartwright, Jr., Ph.D. Assistant Professor of Pathology Baylor College of Medicine Houston, Texas U.S.A.
The "blues" in Richardson"s stains are combined with sodium borate. This brings the pH into a high alkaline level (about pH 12). Most pH meters cannot measure accurately such high readings. Furthermore, it is not that important. There is no one "correct" pH for LM stain for epoxy embedded tissues. Coloration depends upon the fixation, dehydration, processing, and type of tissue components. Most importantly it depends upon the type of epoxide used for embedding. Components of Epon substitutes vary between suppliers. This can make a huge difference in coloration. Faced with trying to match a certain coloration of tissues which have been processed in another laboratory is extremely difficult. Try the following: 1. Suspend the stains (I assume they are from the same company as the original!) in several phosphate buffers of different pH ranges. (5,7,9,11?) 2. Before attempting to do anything at all, soak the sections in warm water for about 30 minutes. 3. After staining be sure to soak the sections for 30 seconds in 75% alcohol, then in 100% for 15 seconds or so. 4. Mounting media may change LM coloration! Use a neutral one such as CYTOSEAL (Fisher).
5. Depending with what and how the sections were embedded, you may have to remove unbound monomers. Contact me if you need the method for that by telephone (303-871-3026). (AM is best).
I tore my hair out (which I can hardly afford) for two years staining semi-thin skin biopsies. I wrote a couple of papers as a result. The bottom line is that LM staining of epoxide embedded sections is extremely complicated!!!!!!!! Try the first 4 items mentioned first. You might also consider giving the stained sections a quick rinse in basic fuchsin. This imparts a pinkish-purplish additive to the final slide.
This really is fun, it just does not seem like it some days! Hildy
To clarify my previous question on Araldite - my collegue is using Araldite(R) Rapide and the similar slower curing version, but what he is really looking for is an adhesive of similar consistency to these but which are inert and preferably insoluble in cyclohexane.
I am having a problem with a new IBM E56 Aptiva Computer. After only about 5 or 10 minutes after first turning it on, and trying to connect to the Internet it crashed, and subsequently doesn't recognize the hard drive. Even the Aptiva Restore CD software is unable to format the drive, and restore the software on the drive.
Someone suggested to me that perhaps the BIOS was damaged by Netscape, but I don't know how to get at this BIOS to fix the problem, and it's a pain to bring it back to the store, (with the long delay in getting back up and running)
Is there anyone who understands this BIOS and who can advise me on how to repair the damage? This is my first experience with PC's runnning windows 95, and my first experience with a crash that I could not recover from. It would be nice to be more self sufficient with regards to computer crash problems, especially if they can be quickly and easily solved right there on the spot, especially with lab computers.
Thankyou, Garry
PS: it also seemed odd to me that IBM's idea for "recovery" is to format the hard drive and reinstall all the software. I would have thought it infinitately preferable to just restore the system software causing the problem, and leave the rest of the files alone.
The timing of this question is pretty good! We have been trying for quite some time to find the right person in NIST to arrange for some form of certification for this sample, because of the frequent requests for ISO-9000-type traceability. Three days ago we finally received a usable letter - the text is included below. The biggest problem for both us and NIST has been that the MAG*I*CAL sample is made from a single crystal of silicon, with the calibration marks being directly traceable to the silicon (111) lattice spacing. This spacing is directly observable on the sample itself through lattice imaging; i.e.: the sample is internally calibrated to a "God-traceable" standard, and NIST generally doesn't bother confirming intrinsic properties of materials. Certification auditors requiring NIST-traceable samples are generally most concerned about adequate paperwork, This recent communication with NIST gives us that paperwork, I believe, as NIST acknowledges the lattice spacings of silicon as being well characterized and documented, making the MAG*I*CAL sample a NIST-recognized sample. We'll be discussing the proper NIST term to use with the MAG*I*CAL sample when Rob Gettings gets back from a business trip in a couple of weeks. In the meantime, a copy of the NIST letter will be available for users of the sample for their certification requirements. If anyone requires a copy of the NIST letter right now, they should contact South Bay Technology, the world-wide master distributor of the sample (fastest), or the vendor from which the sample was purchased (will take longer). The South Bay contact is:
David Henriks South Bay Technology, Inc. 1120 Via Callejon FAX: 714-492-1499 San Clemente, CA 92673 USA TEL: 800-728-2233 (toll-free in USA) TEL: 714-492-2600 (outside USA) e-mail: henriks-at-southbaytech.com
I hope this adequately addresses your question.
Cheers John
John P. McCaffrey Institute for Microstructural Sciences National Research Council of Canada M-50 Montreal Rd. Ottawa, Ontario K1A 0R6 Canada
NIST U.S. Department of Commerce NATIONAL INSTITUTE FOR STANDARDS & TECHNOLOGY
Robert Gettings Standard reference Materials Program Bldg. 202, Rm. 212, Gaithersburg, MD 20899
June 23, 1998
Dear Mr. McCaffrey:
I am sending this communication in regards to your question concerning the calibration of Transmission Electron Microscopes (TEM) using silicon lattice spacings. NIST does not currently supply a standard for calibrating the magnification scale of TEM's, nor for the crystalline lattice spacings of silicon.
The crystalline lattice spacing is an intrinsic property of a material. For pure silicon, the spacing has been well characterized and documented by the scientific community. It is known to 6 decimal places, and can be obtained from the CRC handbook of Chemistry and Physics among other references.
Related work by NIST:
NIST is currently working on SRM 640c Silicon X-ray Diffraction Powder. This material, when finished will provide certified line positions traceable to the SI definition of length.
NIST is also working on a new standard, SRM1990, for the lattice spacing of single crystal ruby. The material will be in the form of a 0.15 mm sphere, and is expected to be complete by the end of this year.
Thank you for your interest in NIST standards.
Sincerely, (signature) Robert J. Gettings Project Manager ----------
I have used the Mag-I-Cal sample that John McCaffrey and crew came up with and which South Bay Technology distributes. This sample covers the full range of Mags available in the TEM. It is done very quickly and simply and it can be used to measure the rotation calibration of the diffraction pattern and the camera constant as well. I have even used it in a High resolution SEM but its use there isn't quite as easy.
Incidentally, they have the Guiness World Record for the World's smallest ruler.
-Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Does anybody have a new or tried and true suggestion for a TEM magnification calibration standard for a mag of 30KX using a CCD camera? I would like to have better than +/- 3% accuracy. Have tried catalase--not happy with it. Latex spheres seem to shrink about 20% (according to a 1968 paper). Diffraction gratings not acceptable--only two to three lines in the image.
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
As an E.M. specialist and ex engineer using computers to run a business y= ou cannot help getting involved with these beasts.
I too have just had a BIOS problem which was solved in a way that may be appropriate to you?
I upgraded my hard drive to find that the old BIOS could not run the upgrade! The way round the problem was to contact the manufacturer's web=
site where there were a number of BIOS upgrades available. I was running= , or trying to run on version 1.4, down loading version 1.5 now has my baby=
running and we are both happy with life.
Good luck, its worth a try!
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
Larry Allard suggested: } One quick idea would be us use something like 10nm colloidal gold particles } and calibrate an image at high magnification using the particle lattice } spacings, then make a (nominal) 30kX image and measure bulk features to get } the actual mag at 30kX. You might want to track the magnification at one } or more steps down the scale as you approach 30kX, to get better accuracy } in the measurement.
I agree. We have had similar standards made for us, with a combination of colloidal gold particles of known sizes placed on a grid. Please contact me back-channel for more information.
Best regards, Steven E. Slap, Vice-President
******************************** Energy Beam Sciences, Inc. The Laboratory Microwave Company http://www.ebsciences.com ********************************
In response to the following question: =============================================== Does anyone know if this John McCaffery calibration standard is NIST tracable or if it has been NIST tested. Russ ================================================ A Certificate of Calibration accompanies each unit of the product. The complete statement, as well as extensive additional information about the product, can be found at URL http://www.2spi.com/catalog/standards/mag-cert.html
The introductory paragraph that is on the Certificate is the following: ++++++++++++++ With regard to the tracability and certification of the MAG*I*CAL™ calibration sample, each sample is grown on {001} oriented single crystal silicon, and all spacings on the sample are directly referenced to the cross -sectional (111) lattice spacing of silicon on the sample itself. This spacing is visible by lattice imaging on the sample itself, allowing each sample to be self-calibrating. Each unit comes with a numbered certificate, the text of which is included below. This certificate has been used for ISO 9000 certification, with the argument that the sample is self- calibrating against the (111) lattice spacing of silicon (a fundamental constant), and to our knowledge, this is the highest quality TEM calibration sample available anywhere in the world at this time. ++++++++++++++++++++++++++++++ I hope that this information answers your question.
Disclaimer: SPI Supplies has been distributing this product (as well as others) and full details are available on our website given below.
Chuck
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I've seen this sort of problem with several newer computers. The source of the problem is not clear. In one case the FAT (file allocation table) was corrupted necessitating a reformat. The best solution I've found is to install Norton Utilities and follow their advice for creating rescue disks, running Image on startup etc. There are many resources for information on the Web that are more appropriate (than this Microscopy forum) for discussing this sort of general computer issue.
Dear Chris: I assume you are looking for: Moxtek (soft x-ray technologies) 452 West 1260 North Orem, UT 84057 Dr. Clark Turner 801-225-0930 801-221-1121 FAX moxtek-at-moxtek.win.net
Yours Igor Ivanov Senior Scientist RJ Lee Group, Inc. Analytical Services 530 McCormick Str San Leandro, CA 94577 510-567-0480
On 06/26/98 16:04:38 you wrote: } Sorry finger slipped on the keys. } Message should be Moxtek not Mostek. } Anyone have a phone number please. } } Chris Gilpin } Biological Sciences Electron Microscope Unit } G452 Stopford Building } Oxford Road } Manchester } M13 9PT } phone +44 161 275 5170 } fax +44 161 275 5171 } http://www.biomed.man.ac.uk/biology/emunit/emhome.html } } }
Offhand, I would be suspicious of the cooling fan/system. I have seen a number of computers get flaky once the processor overheated. My first experience of that type was after installing a 486-50 overdrive processor in a PS/1. (Nobody told me it needed a cooling fan.) Things come to a halt very quickly once the processor overheats, and it doesn't take all that long to warm up. 5-10 minutes sounds like plenty long enough. I have had the same experience with trying to drive a processor a little to hard, like when I was trying to push my 120 MHz up to 133 MHz. It didn't seem like much of a push, but the manufacturers have already well pushed to the edge so that there is no room for a cooling fan to fail
If there is a cooling problem, I would not be surprised if BIOS setup also fails to function. Is there anything on it that works? Can you boot from a floppy?
If it does need a refresh of the BIOS, many companies provide files that you can download from the internet. You use them to prepare a bootable floppy which is then used to "flash" the BIOS on the subject computer. It seems daunting, but I succeeded on updating the BIOS on an HP Vectra on the first try. Fortunately, it wasn't as dead as yours, but that really shouldn't matter.
Warren
At 11:00 AM 6/26/98 -0500, Gary Burgess wrote: } } I am having a problem with a new IBM E56 Aptiva Computer. After only } about 5 or 10 minutes after first turning it on, and trying to connect } to the Internet it crashed, and subsequently doesn't recognize the hard } drive. Even the Aptiva Restore CD software is unable to format the } drive, and restore the software on the drive. } } Someone suggested to me that perhaps the BIOS was damaged by Netscape, } but I don't know how to get at this BIOS to fix the problem, and it's a } pain to bring it back to the store, (with the long delay in getting back } up and running) } } Is there anyone who understands this BIOS and who can advise me on how } to repair the damage? This is my first experience with PC's runnning } windows 95, and my first experience with a crash that I could not } recover from. It would be nice to be more self sufficient with regards } to computer crash problems, especially if they can be quickly and easily } solved right there on the spot, especially with lab computers. } } Thankyou, } Garry } } PS: it also seemed odd to me that IBM's idea for "recovery" is to format } the hard drive and reinstall all the software. I would have thought it } infinitately preferable to just restore the system software causing the } problem, and leave the rest of the files alone. }
Are you referring to the limitation of the old computers to access disks larger that 540M without an enhancement to the BIOS?
If so, most large disks (used to) come with a BIOS extender to allow access of the whole disk. Perhaps manufacturers figure the old BIOS systems are all gone and such utilities are no longer needed. But if a BIOS upgrade is available for your system to allow large disk access, then that would certainly be the better way to go.
Warren
At 12:24 PM 6/26/98 -0400, Steve Chapman wrote: } } Hi Gary, } } As an E.M. specialist and ex engineer using computers to run a business you } cannot help getting involved with these beasts. } } I too have just had a BIOS problem which was solved in a way that may be } appropriate to you? } } I upgraded my hard drive to find that the old BIOS could not run the } upgrade! The way round the problem was to contact the manufacturer's web } site where there were a number of BIOS upgrades available. I was running, } or trying to run on version 1.4, down loading version 1.5 now has my baby } running and we are both happy with life. } } Good luck, its worth a try! } } Steve Chapman
I would like to discuss anyone's experience using Raman micro-spectroscopy to get chemical information on particles. We are exploring the possibility of using the technique to provide information on nitrates, sulfates, phosphates, carbonaceous particles, hydrated particles etc., to complement present characterization of micron-sized particles by SEM and EDS.
I would also be interested in user's experiences with raman microscope systems that are commercially available. Thanks to all respondents.
Bob Willis ManTech Environmental Technology, Inc 2 Triangle Drive Research Triangle Park, NC Email: rwillis-at-epamail.epa.gov
The messages following my comments are some dated email messages regarding the Polaroid Sprintscan 45. It will do what you want it to do.
I bought one and am fairly pleased with it. I had some problems with the vendor getting it to me; it took about 4-5 months even though Polaroid had them in stock (this was told to me by a Polaroid rep). When the unit came in, many of the parts were missing: manuals, SCSI cable, negative carriers, power cord, and SCSI terminator. It took about a week to get the essential parts to get it working. It took much longer to get the TEM negative carrier and I never did get the SCSI cable that was supposed to be in, but I had another and so it was not an issue and I let the matter drop.
The TEM carrier that they eventually sent me is not the glass one with the anti-Newton glass that is described somewhere below. It is a pressed metal piece with two magnets that fits into the 4x5 holder. It is not as convenient as the 4x5 holder and the magnets crop off a little of the image. John Warren recommends that the magnets be cut long way to avoid this, but I haven't done this yet. However, I think that they are close to a good negative carrier with this metal piece if they were to replace the spring loaded plate on the 4x5 that has a rubber mat around the edges of the 4x5 hole on it with a TEM negative sized plate. This then would come down on the insert plate that they are now using with the magnets and eliminate the magnets. I think that this arrangement would be as easy to use as the original 4x5 holder. I'm planning on having our machine shop make one. This plate is held in place by four posts, springs, and snap rings on the posts.
It works very well and it is very fast. I still have a little trouble setting the correct scan conditions. I think that their scan software needs some work, though. I have had trouble getting the right scan conditions on some negatives. I like to use a histogram to set up the conditions, but it only gives a few sporadic lines and I haven't figured out why this happens. It took a little while to get it set up. I have a umax flatbed scanner on the system and the Polaroid software had trouble recognizing the device even though all of the SCSI devices, including the 45, were on line. This seems to be a minor irritant with Polaroid scanners in general because another system down the hall has a Sprintscan 35 and a Polaroid flatbed. I got that software to recognize the flatbed on that system by turning off the Sprintscan35. I basically did the same thing on our system and turned the flatbed off and it finally recognized the Sprintscan45. We solved the problem when we found that the TWAIN source selected was our umax32 source instead of the umax16 source while the TWAIN32 was using the Sprintscan45 source. Now we select the TWAIN32 device as either the umax32 or the Sprintscan45 and we can leave both devices on.
The Sprintscan 45 doesn't have a timed out feature and the light stays on all the time. That's a bit of a problem because I like to leave this system powered up all the time.
Generally, I'm very pleased with the system and use it often.
-Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Does anyone know if a TEM negative carrier is available for the Polaroid Sprintscan 45 Negative Scanner yet? Anyone having any experience with the carrier (if it exists) and this scanner, could you send me a little message on what you think of the system?
Thanks.
-Scott Walck
Walck-at-PPG.com
Scott D. Walck PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
I have had many conversations regarding digital imaging with a wide variety of end users. The points made here have been validated in those conversations. I thought that the point of view from law enforcement might be of interest.
When a photographer or law enforcement agent testifies as to what is seen in a given image, they are attesting to the fact that the image is an accurate _representation_ of what they saw and photographed at a given point in time at a given location. Based on the perceived reliability of the witness, a judge or jury determines whether that testimony is accurate.
There has been much discussion in Law Enforcement circles about digital watermarks and the like that would 'disappear' if an image were manipulated. One well known digital camera manufacturer who indicated that they had a proprietary file format that could not be manipulated was corrected by a hacker. Given the right equipment and talent, an analog film image could be scanned in, manipulated and output back to film and the best experts in imaging could not be able to tell and they will admit that. Someday, it may be possible, but not today. So, my point is that any image, digital or analog, is only as factual or accurate as the integrity of the person representing it as such.
John D. Warren Area Sales Manager Digital Products Polaroid Corporation "See What Develops" 4525 Leonard Parkway Richmond, Virginia 23221-1809 804 254 1011 804 254 1013 Fax warrenj1-at-polaroid.com
The PDC 2000/40 has a new(45 days old) list price of $1699 and the PDC/2000/60 has a list price of $1999. There has not been a price reduction on the DMC as of yet.
John D. Warren Area Sales Manager Digital Products Polaroid Corporation "See What Develops" 4525 Leonard Parkway Richmond, Virginia 23221-1809 804 254 1011 804 254 1013 Fax warrenj1-at-polaroid.com
Right, prices seem to be dropping. I recently visited one web site advertising the PDC-2000 for ~$2500 only to visit again a few weeks later to see it reduced to ~$1600. I haven't seen a comensurate price drop in the DMC 2000 but that is probably because I haven't recieved a recent quote. Kevin Brent Smith University of Louisville Biology Dept.
-----Original Message-----
For the record, the Polaroid PDC-2000 is available for use either "tethered" or "untethered", the latter with onboard storage for 40 or 60 images depending on the model. You will then need to connect to the computer to download your images, though. Price of the PDC-2000 is now under $2K for all models.
Hope this helps your information gathering process. For more, get back to me or check www.polaroid.com
John, As you know, I bought the Sprintscan 45. You know that I had first contact you about this and had several inputs from the microscopy listserver. I had a very long delay in getting the thing to me and when it finally arrived, there were parts missing. You had me contact Dennis Lizier and he sent out the standard parts for the scanner (sans cable, but I already had one). However, I told him that you had said that the TEM negative carrier with the antireflection glass plates would also be sent to me. I haven't received it. I have left several messages with him about this, but I have not gotten a response or a call back. The original order went out in Jan. We have paid for the unit with the good faith that the carrier would be delivered. Am I going to receive the negative carrier? Can you please help me? -Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
we want to buy a scanner which can be used for both 35 mm and 70 mm EM film as well as 81x100 mm plates, probably with different adapters (?). Software must be compatible with Windows 95/NT 4.0 (e.g Adobe, Freehand...). The higher resolution the better (at least 1200 dpi), the cheaper the better, as usual. Any helpful comments or experience? - would be highly appreciated. Thanks a lot,
I am a computer guy. Working on them, building them and repairing them is the work that I do every day so I can squander my pay on my real love -- microscopes.
If your computer comes back after turning it off an setting for a while, the bios [basic in/out system] is not the problem. You are getting information to the computer and out of the computer. It sounds as if you have a cooling fan problem. The processor gets overheated and then refuses to work. Letting it cool will allow it to function for a few more minutes and then it will refuse to work again. Depending on the CPU, you may be beyond the point of needing to replace it already. Look for extended troubles down the line. You can put a diagnostic on it and watch it slow down as it overheats.
Get the largest fan and heatsink set up you can for the CPU. Make sure it is a a ball bering fan. Sleev fans freeze up too easily. We sell very nice ones for $8.00. I am not soliciting your business, but pointing out that you should not pay an arm and a leg for a good one. $12.00 should get you the fan and heatsink you need. The main reason for this problem is dirt. Clean out your machine every so often -- three times a year. If it sets on the floor -- keep a closer eye on it. There is a lot of dust down there.
I am sure I live a long way from you, and it is difficult to diagnose problems without looking at them. Any number of problems may be occuring. If the machine flakes out on you at about the same interval without being on the internet, it may be the CPU cooling system. If the machine only fails while on the internet, it probably is something to do with the modem or the phone line or the intenet.
You are undoubtedly "a man of science and technology", apply the scientific method to it. If you are a biologist who believes in evolution rather than creation, make up you own story and set out to prove your theories correct because surely faith in the manufacturer of the piece has nothing to say about its oporation parametes.
Best of luck, Gary. Let us all know how it turned out!!
I am not lover of Microsoft though I use their products because they are the best system going. I have tried others. Please don't blame them for the fact that you do not back up. This is a very inexact science still. Back ups are the most important detail that most people ignore. MS has a good backup utility - especially on '95. Either use that or tape or disk media or best of all; with the prices of hard drive dropping drastically, put another large drive in you system and use it to back up your files.
Another piece of advice, stay away from the proprietary stuff the next time around. They can make for some wicked expensive repairs. Go to a computer shop and have them build you a clone. Every piece in it is replaceable with bigger, better and newer hardware. You will be much better pleased with the service and repair turn arounds too.
I am preparing to make microscope slides of palm flowers and fruits. Some of the fruits are quite woody as are many of the developmental stages of the flowers. I had planned to use the old parafin method of microtome preparation but I have been advised to consider resin. My funds and resources are limited, but the resin method seems to have more advantages.
Questions: 1. Can I use a manual rotary Spencer 820 American Optical Corp Microtome on resin samples?
2. What are the drawbacks to using the resin method?
3. What are the drawbacks to using the parafin method (toxicity/results/time)?
Thanks in advance for any and all responces. Please reply to my email: crow-at-aloha.net
I will be sectioning palm flowers and fruits, some of which are almost woody. I had planned to use the old parafin method. The microtome I have in my lab is a rotary Spencer 820 (manual) , American Optical Corporation. Resin preparation instead of parafin has been recommended to me.
1. Can I use the above rotary microtome to section resin prepared palm fruit material?
2. What are the benefits vs down sides of resin/parafin
3. What is the best type of prep procedure for using resin (books/references/labs?)
Thanks in advance for any ideas and experience. Please reply to my email:
crow-at-aloha.net Mattie
_______________________________________________________________ Melany H. Chapin
To Reply Please Remove Spam-Proof "XXX" from return address.
ADDRESS: crow-at-aloha.net
After dark, all cats are leopards. (Zuni proverb) We will be known by the tracks we leave. (Dakota) When the legends die, the dreams end; there is no more greatness (Shawnee) Every animal knows more than you do. (Nez Perce) ________________________________________________________________
We were not quite ready to announce this NIST information as we still hav= e some clarification left to do, but now that the word is out, everyone see= ms to want/need a copy of "the letter". With that in mind, and because of t= he number of requests I have received already, I will be posting a copy of t= he letter on our web site (http://www.southbaytech.com) within the next week= =2E =
When I have the exact page address, I will post it here.
It seems from my inspection of the BIOS that it was indeed set OK, and that Netscape didn't screw it up. However, it still would not boot up. The store was kind enough to exchange the computer for a new one though, which I appreciated, since it was only 5 minutes out of the box until I had this problem. My suspicion is that there was a bad -very bad sector on the hard drive that caused this nightmare.
Anyway, I would like to thank everyone who took the time to give me some suggestions, some of which were to get a new computer. I've learned something anyway.
Garry
} ---------- } From: Terry D. Krueger[SMTP:tdkrueger-at-imation.com] } Sent: 29 June, 1998 08:28 } To: Garry Burgess } Subject: Re: Computer BIOS Problem } } If I was sitting in front of your computer it would be a lot easier. } However, here goes. } When you turn the computer on do you see the RAM memory test and all that } stuff? You should see a message that says "Press {Delete} to enter setup." } That will get you into the Bios. Then all you can do is check to see if } the correct parameters are entered in the Bios to match the specifications } of you hard drive. The specifications of your hard drive should be printed } on a label on the drive itself, so you may have to take the box apart and } look at it. First you must make sure that a hard drive is entered at all. } You should be able to select that somewhere on the left side of the screen. } Then you must enter all the parameters on the right side of the screen such } as # of heads, # of sectors, Meagbytes or Gigabytes. My experience is with } slightly older equipment and I am assuming that there aren't any major } differences in this IBM aptiva Bios. If everything looks OK, you may just } have a loose ribbon cable going to the drive. It would be quite surprising } to have Netscape screwing up your Bios. The purpose of your Bios is just } to tell the computer what kind of hardware you have and how to use it. } There is nothing in Netscape that I have heard of that could reprogram it. } } }
Has anyone ever observed anything similar to this:????If so how did you fix it???
I have a filament image vibration in one direction at 30-60hz in a JEOL 4000. It is very subtle. I have observed this for 3 years but the amplitude has increased such that we would like to pin down the source and correct it.
Since initially observing the problem we have had a gun change, several filament changes, the high voltage tank worked on so I do not see these as probable causes. Also I do not think these would give a one direction effect. The magnetic fields of computers, monitors, and step motors do not seem to have any effect on the filament image vibration.
I am thinking that the deflector circuits would be most suspect. Usual tests on the power supplies look good. Any ideas would be appreciated. -- Dr. Roseann Csencsits Electron Microscopy Center Building 212/C217 Argonne National Laboratory 9700 South Cass Avenue Argonne, IL 60439-4838 Phone: (630) 252-4977 Fax: (630) 252-4798
Dear Roseann, } } Has anyone ever observed anything similar to this:????If so how did you } fix it??? } Our HVEM has had similar problems, which we found were caused by mechanical vibrations--the column resonates at 20 Hz. We determined that the vibrations arose from many small sources, and when we fixed every- thing we could think of (including when the bearings for the air condi- tioning fans for our 45 story building were replaced), the vibrations lessened and finally are no longer a problem.
} I have a filament image vibration in one direction at 30-60hz in a JEOL } 4000. It is very subtle. I have observed this for 3 years but the } amplitude has increased such that we would like to pin down the source } and correct it. } Does your column resonate in the 30-60 Hz region? 60 Hz would seem at first thought to be electrical, but maybe not. We had someone come in with a vibration generator, and when he swept through the fre- quency range while I was looking through the binoculars, I could see a change from quiescence to large motion as the frequency went through 20 Hz.
} Since initially observing the problem we have had a gun change, several } filament changes, the high voltage tank worked on so I do not see these } as probable causes. Also I do not think these would give a one } direction effect. The magnetic fields of computers, monitors, and step } motors do not seem to have any effect on the filament image vibration. } } I am thinking that the deflector circuits would be most suspect. Usual } tests on the power supplies look good. } Any ideas would be appreciated. } -- Sounds like electrical causes have been checked out. If your instrument is shock-mounted, it will be sensitive to acoustic vibrations, so you may have to look into air conditioning vents, etc. Good luck; tracking down this kind of subtle effect--which may arise from several sources--can be devilishly frustrating. When you fix one source, there may be little or no observable improvement; only when you fix lots of things will you notice a gradual improvement. Yours, Bill Tivol
Does anyone have information about this stain? What is its use in TEM? What is the procedure in it's use? One of the project leaders thought it would be good to use but didn't know why. Thank you for your help. PAM
I am in a mild state of confusion regarding an immuno-EM experiment where it seems that the secondary antibody (GAM 5 nm) does not recognize the primary (mouse IgG) once the primary is bound to its corresponding antigen in tissue. We expect that the antigen is present with periodicity on fibrils in the connective tissue matrix. After the tissue is emersed in antibody, we see periodic decoration of the fibrils, which indicates to us that the antibody is bound. However, secondary antibody does not bind to the tissue. We are convinced that the secondary conjugate is not defective, as we use it for other experiments. Has anyone else experienced a situation where a secondary does not recognize a primary once the primary is bound to its corresponding antigen?
Many thanks for your consideration,
Doug ---------------------- Douglas R. Keene Associate Investigator Shriners Hospital Microscopy Unit Portland, Oregon 97201 DRK-at-shcc.org
My name is Steve Short. I work for Technifab Products, Inc., a manufacturer of cryogenic equipment located in Brazil, Indiana. I am researching the use of detector dewars in electron microscopes and other microscopy related equipment.
If you have any basic information regarding the fundamental operation of electron microscopes and more specifically the role of liquid nitrogen detector dewars, please notify me as to how I may obtain that information.
Tel. 812-442-0520 e-mail to sms-at-technifab.com Fax 812-442-0891
A quick comment on your difficulty setting scan conditions for negatives: Scanner software often applies a gamma correction for negative scans, apparently to compensate for the low contrast of negative film used in conventional photography. With slow programs, you can sometimes see the scanned image change as built-in lookup table is applied after the scan is complete. Also, your scanner control software might offer a choice of film settings (Kodak, Agfa, ...). That's another tipoff that the scanner software is applying a lookup table after scanning. TEM negatives often have very high contrast, and aren't really the kind of negatives the scanner programmers had in mind. A common result of scanning TEM film as negatives is severe posterization (loss of gray levels).
To avoid this effect, try scanning the TEM film as positive transparencies (you can invert the contrast with other controls in the scanner software, or in Photoshop. Also, in the positive transparency mode (for Microtek scanner software at least), there is a further option to force a linear gamma correction. I sometimes find this mode useful for scanning extremely high contrast negatives.
Larry Thomas Mechanical and Materials Engineering Washington State University Pullman, WA 99352 email thomas-at-mme.wsu.edu
---------- From: Walck. Scott D. Sent: Saturday, June 27, 1998 12:41 AM To: matthias.morgelin-at-medkem.lu.se; Micro Subject: RE: negative scanner -Polaroid Sprintscan45 and collected previou s messages.
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
The messages following my comments are some dated email messages regarding the Polaroid Sprintscan 45. It will do what you want it to do.
I bought one and am fairly pleased with it. I had some problems with the vendor getting it to me; it took about 4-5 months even though Polaroid had them in stock (this was told to me by a Polaroid rep). When the unit came in, many of the parts were missing: manuals, SCSI cable, negative carriers, power cord, and SCSI terminator. It took about a week to get the essential parts to get it working. It took much longer to get the TEM negative carrier and I never did get the SCSI cable that was supposed to be in, but I had another and so it was not an issue and I let the matter drop.
The TEM carrier that they eventually sent me is not the glass one with the anti-Newton glass that is described somewhere below. It is a pressed metal piece with two magnets that fits into the 4x5 holder. It is not as convenient as the 4x5 holder and the magnets crop off a little of the image. John Warren recommends that the magnets be cut long way to avoid this, but I haven't done this yet. However, I think that they are close to a good negative carrier with this metal piece if they were to replace the spring loaded plate on the 4x5 that has a rubber mat around the edges of the 4x5 hole on it with a TEM negative sized plate. This then would come down on the insert plate that they are now using with the magnets and eliminate the magnets. I think that this arrangement would be as easy to use as the original 4x5 holder. I'm planning on having our machine shop make one. This plate is held in place by four posts, springs, and snap rings on the posts.
It works very well and it is very fast. I still have a little trouble setting the correct scan conditions. I think that their scan software needs some work, though. I have had trouble getting the right scan conditions on some negatives. I like to use a histogram to set up the conditions, but it only gives a few sporadic lines and I haven't figured out why this happens. It took a little while to get it set up. I have a umax flatbed scanner on the system and the Polaroid software had trouble recognizing the device even though all of the SCSI devices, including the 45, were on line. This seems to be a minor irritant with Polaroid scanners in general because another system down the hall has a Sprintscan 35 and a Polaroid flatbed. I got that software to recognize the flatbed on that system by turning off the Sprintscan35. I basically did the same thing on our system and turned the flatbed off and it finally recognized the Sprintscan45. We solved the problem when we found that the TWAIN source selected was our umax32 source instead of the umax16 source while the TWAIN32 was using the Sprintscan45 source. Now we select the TWAIN32 device as either the umax32 or the Sprintscan45 and we can leave both devices on.
The Sprintscan 45 doesn't have a timed out feature and the light stays on all the time. That's a bit of a problem because I like to leave this system powered up all the time.
Generally, I'm very pleased with the system and use it often.
-Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
From: Walck. Scott D. To: Micro Subject: Polaroid Sprintscan 45 Negative Scanner- Date: Wednesday, November 12, 1997 6:51PM
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Does anyone know if a TEM negative carrier is available for the Polaroid Sprintscan 45 Negative Scanner yet? Anyone having any experience with the carrier (if it exists) and this scanner, could you send me a little message on what you think of the system?
Thanks.
-Scott Walck
Walck-at-PPG.com
Scott D. Walck PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
From: alline.myers To: gls4590 Subject: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier Date: Thursday, November 13, 1997 11:09AM
Scott,
Hi. We have a Polaroid Sprintscan 45 that I use for scanning TEM negatives. It's a great scanner, and scans both high res. images and diffraction patterns well. We took the 2x2-inch negative adaptor, the film carrier, and a TEM negative to the shop at NIST and had them make an adaptor for us. It works fine. I don't think Polaroid has made an adaptor for TEM negtives yet. Let me know if you need more info.
As you can tell, I'm at NIST now and no longer working for Dr. Hren, although I still do TEM on samples for him. I had heard you had changed jobs a few months ago. How do you like Pittsburgh?
From: alline.myers To: Walck. Scott D. Subject: RE: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier Date: Wednesday, November 19, 1997 12:23PM
} } Do you ever have problems with optical density in terms of bright and dark } areas of the negative? How long does it take to do a TEM negative at full } resolution?
The optical density is 3.4 and so far I haven't had trouble with it --- can see faint details in diffraction patterns. The problem comes when I print to the dye-sub printer; it can't reproduce everything I can see on the computer screen.
I can scan a full B/W negative at 2000dpi in 2.5 minutes; this is around a 40MB image. You need 2.5 times the image size free on your scratch disk (in Photoshop) to scan an image. If I scanned the same negative at 4000dpi (max. possible), it would be a 160 MB file!!! I haven't tried this, mainly do to space limitations on my computer. Usually I scan a 2-inch square region of interest at 2000dpi, store that, then reduce the image to 400 dpi for further work and to 150dpi to print.
From: bozzola To: Walck. Scott D. Subject: Re: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier? Date: Thursday, November 13, 1997 7:53AM
} Does anyone know if a TEM negative carrier is available for the Polaroid } Sprintscan 45 Negative Scanner yet? Anyone having any experience with the } carrier (if it exists) and this scanner, could you send me a little message } on what you think of the system?
Hi Scott,
I have been evaluating one for almost a month now and I am moderately pleased with it. It consists of a composite plastic frame with a single pane of anti-Newton glass onto which you place the negs. They suggest using a sticky paper tape (as Post-It adhesive) to ensure non-movement of the neg during scanning. I was very dubious about this arrangement but it has worked out surprizingly well. I feel that a "kit" should be provided consisting of the main frame, the glass and several plastic or metal inserts to act as negative carriers. That way, one could select the best combination of components for glassless or conventional scanning.
I am very pleased with the SprintScan. The resolution is good, the plug-in software (Macintosh) works well in concert with PhotoShop and is simple to use. Although I bought the unit without ever trying it out (gulp!) I was assured that I could return it if not satisfied. Well, we're going to keep it. I can not compare it to other scanners such as the Agfa but I feel that it is a fine piece of equipment and should serve us well. Although we have a Gatan camera on our TEM, the quality of the images are inadequate for publication. So, we have opted to generate conventional negs and to by-pass the darkroom by scanning and then dye-sub printing the prints. We have been using the Fargo printer several years now but eagerly await delivery of the new Codonics 1660 printer which should be out towards the end of December. We ordered it nearly a year ago but it has been slow to materialize since it represents a new technology (direct thermal line printing) as well as a conventional dye sub unit. I hope the wait is worth it, because we have had to refer many people to Kinko's for the best quality prints.
Judy Murphy was thinking of buying one also. You might contact her at either murphy-at-inreach.com OR murphy-at-ms.sjdccd.cc.ca.us
Contact me if you have more specific questions.
John
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy Neckers Building, Room 146 - B Wing Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
From: colin.veitch To: gls4590 Subject: RE: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier? Date: Thursday, November 13, 1997 1:52PM
Hi Scott.
We purchased the 45 not long ago and have found it very good. The holders that come with the system are almost the right size (for our negs) and we haven't had to "modify" or build new ones yet. I imagine that building the correct sized insert for the holder would not be too difficult.
The plug-in interface to Adobe Photoshop (we are running it on a PC) is good, with several different ways of altering scan characteristics ie brightness contrast etc..
Scan times are good, less than a minute for a 35mm up to around 5 minutes for a full sized (6cmx9cm) neg at 4000 dpi.
We have had a couple of minor crashes of the system but these occurred while the system was logged on to the network and scanning at the same time. No net logon, no problem!!
We've been happy with the system so far, just be warned that if you are going to scan large negs at high resolution you'll need lots of drive space and RAM. We have 128Mb RAM and with a full colour large neg you can notice a slow down in the system!!
I've no association with Polaroid or the agents in Australia, just a happy customer!!
Good luck,
Colin V.
Colin J. Veitch Instrumentation Scientist CSIRO Division of Wool Technology PO Box 21, BELMONT, Vic. 3216. Australia.
E-mail: colin.veitch-at-dwt.csiro.au
Tel: +61 (0) 3 5246 4000 Fax: +61 (0) 3 5246 4811
} Does anyone know if a TEM negative carrier is available for the } Polaroid } Sprintscan 45 Negative Scanner yet? Anyone having any experience with } the } carrier (if it exists) and this scanner, could you send me a little } message } on what you think of the system? } } Thanks. }
From: Hendrik O. Colijn To: Walck. Scott D. Subject: Re: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier? Date: Thursday, November 13, 1997 8:28AM
Hi Scott,
I'm considering getting the SprintScan 45 too. Let me know what you think.
Thanks, Henk Colijn
} } Does anyone know if a TEM negative carrier is available for the Polaroid } Sprintscan 45 Negative Scanner yet? Anyone having any experience with the } carrier (if it exists) and this scanner, could you send me a little message } on what you think of the system? } } Thanks. } } -Scott Walck } } Walck-at-PPG.com } } Scott D. Walck } PPG Industries, Inc. } Guys Run Rd. (packages) } P.O. Box 11472 (letters) } Pittsburgh, PA 15238-0472 } } (412) 820-8651 (office) } (412) 820-8161 (fax) } } } "The opinions expressed are those of S.D. Walck and not of PPG Industries, } Inc. nor of any PPG-associated companies." } Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 Murphy's Law: "If anything can go wrong, it will." Commentary: "Murphy was an optimist."
From: Pauline C. Yu To: Walck. Scott D. Subject: Re: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier? Date: Thursday, November 13, 1997 8:41AM
I was just asked that very question the other day by my supervisor. We just purchased a sprintscan 45 and have taken a long time to get it set up. It came with a bunch of adapters(including a 2"x2.5" holder) but none in that special TEM size. I've considered just improvising a frame to fit into the 4x5 holder to hold the TEM negs; I also think I might be able to find someone who could just machine one for me. Maybe if enough people request it, Polaroid will make one, or some enterprising EM supply company will create one... As for the Scanner, it requires one of the newer SCSI boards, and a really up to the minute computer if you don't want to wait too long. We're running it on a WinNT4 with dual processors, and it's hardly a "sprint-y" scan. But as far as image quality goes, I've been rather impressed. The color is rather off, so it does require color management(though if you're doing just b/w TEM, it's nothing to worry about). The driver is integrated into whatever photo-manipulation program you have(they expect you to have Photoshop, but it works with Corel PhotoPaint and PhotoHouse too).
From: bozzola To: Walck. Scott D. Subject: Re: Polaroid Sprintscan 45 Negative Scanner-TEM film carrier? Date: Friday, November 14, 1997 2:58AM
} Thank you very much for the info. This was just what I was looking for. } One question: when you ordered it, how did you get info about the TEM } negative carrier? I see the SprintScan 45 advertised in a couple of mail } order catalogs and I'm sure they won't know what I'm talking about if I call } them.
What happened was:
When we received the SS 45 I quickly ascertained that a TEM carrier was needed and so I called the technical support guys asking if one was available. They didn't know what I was talking about and referred me to several other admin types who finally told me that none was available. I then asked if they would provide me with some of the components from the 4x5 carrier so I could have one fabricated and they flatly said no. End of discussion. Being a bit disturbed by this attitude, I posted a message to the MSA server complaining about this. About three weeks later I got a call from some VP at Polaroid who had just come from a round of golf with Charles Garber of SPI. Charlie relayed my complaint to him with the admonition that SPI might even sell the unit if it had a TEM carrier. Anyway, the VP said that they would be "working on a solution". Right .... I thought. Well, they actually did develop one and John Warren of Polaroid contacted me to see if I would evaluate one of the first designs (the one I described to you in the earlier note). I was supposed to return it quite some time ago, but it has been in use and I hate to go back to using the 4x5 glassless carrier for the TEM negs. So, I am hoping they will leave it with me ....... .
Anyway, a unit is soon to be released and if you check with John Warren at WARRENJ1-at-CLIFFY.POLAROID.COM he may have more info on the availability. Like I said, it works fine as is but with a few simple modifications it will be great. I'll probably get in trouble for telling you about this "prototype" but you can mention that it makes a difference in your decision to buy and maybe they will forgive my indiscretion.
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I have had many conversations regarding digital imaging with a wide variety of end users. The points made here have been validated in those conversations. I thought that the point of view from law enforcement might be of interest.
When a photographer or law enforcement agent testifies as to what is seen in a given image, they are attesting to the fact that the image is an accurate _representation_ of what they saw and photographed at a given point in time at a given location. Based on the perceived reliability of the witness, a judge or jury determines whether that testimony is accurate.
There has been much discussion in Law Enforcement circles about digital watermarks and the like that would 'disappear' if an image were manipulated. One well known digital camera manufacturer who indicated that they had a proprietary file format that could not be manipulated was corrected by a hacker. Given the right equipment and talent, an analog film image could be scanned in, manipulated and output back to film and the best experts in imaging could not be able to tell and they will admit that. Someday, it may be possible, but not today. So, my point is that any image, digital or analog, is only as factual or accurate as the integrity of the person representing it as such.
John D. Warren Area Sales Manager Digital Products Polaroid Corporation "See What Develops" 4525 Leonard Parkway Richmond, Virginia 23221-1809 804 254 1011 804 254 1013 Fax warrenj1-at-polaroid.com
From: WARRENJ1 To: Microscopy-at-sparc5.microscopy.com; Kevin Brent Smith Subject: Re[4]: digital camera (PDC-2000: Tethered vs. Untethere Date: Thursday, December 11, 1997 2:39PM
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The PDC 2000/40 has a new(45 days old) list price of $1699 and the PDC/2000/60 has a list price of $1999. There has not been a price reduction on the DMC as of yet.
John D. Warren Area Sales Manager Digital Products Polaroid Corporation "See What Develops" 4525 Leonard Parkway Richmond, Virginia 23221-1809 804 254 1011 804 254 1013 Fax warrenj1-at-polaroid.com
______________________________ Reply Separator _________________________________ Subject: RE: Re[2]: digital camera (PDC-2000: Tethered vs. Untethere Author: Kevin Brent Smith {kbsmit01%homer.louisville.EDU-at-prdnet.polaroid.com} at INET Date: 12/11/97 12:40 PM
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Right, prices seem to be dropping. I recently visited one web site advertising the PDC-2000 for ~$2500 only to visit again a few weeks later to see it reduced to ~$1600. I haven't seen a comensurate price drop in the DMC 2000 but that is probably because I haven't recieved a recent quote. Kevin Brent Smith University of Louisville Biology Dept.
-----Original Message----- From: R-Brooks Corl [SMTP:CORLB-at-cliffy.polaroid.com] Sent: Thursday, December 11, 1997 11:44 AM To: Kevin Brent Smith; kszaruba-at-MMM.COM Cc: Microscopy-at-sparc5.microscopy.com; YONG SUN; CRAIG ARMSTRONG Subject: Re[2]: digital camera (PDC-2000: Tethered vs. Untethered)
For the record, the Polaroid PDC-2000 is available for use either "tethered" or "untethered", the latter with onboard storage for 40 or 60 images depending on the model. You will then need to connect to the computer to download your images, though. Price of the PDC-2000 is now under $2K for all models.
Hope this helps your information gathering process. For more, get back to me or check www.polaroid.com
From: WARRENJ1-at-POLAROID.COM To: Walck. Scott D. Subject: Re: Sprintscan 45 Date: Wednesday, May 13, 1998 10:04PM
Scott
As I had indicated, our product development was working on this and they have been promising it on a regularly basis. I forwarded your message and it seems that they are still working on a glass carrier. however, they have a solution in the meantime. Here is a clip of the message I got yesterday.
} } Yes, I have some good news. I have a new TEM film holder in my hands. This is a metal mask that fits into the 4x5 holder, just like the other masks. I have 9 to be exact and, for right now, they are for emergency sales only. We are making out a plan to make more and the number depends on the amount you think you need.
I am sending one to John Bozzola, our first SprintScan 45 TEM customer. He was very helpful with feedback. He said the glass holder was okay, but they preferred and open air solution because most of their picture taking process avoids the use of glass completely. He sounded quite excited about this holder.
Again, this mask works like the others. It is placed into the 4x5 holder and a magnet is required. This mask is easier to use because it has knobs on two sides of the holder enabling the user to place the film more square into the holder. My only recommendation is to cut one of the magnets in half; the long way, to use it properly.
This holder is not the glass holder we have been working on. The glass holder is in the last stages, and we should see something in the next couple of weeks. { { {
Dennis Lizier may have contacted you about this as well but if not he is getting one of these to send to you.
Thanks for your patience and support.
John
______________________________ Reply Separator _________________________________ Subject: Sprintscan 45 Author: "Walck. Scott D." {walck%ppg.COM-at-prdnet.polaroid.com} at INET Date: 5.11.98 7:15 PM
John, As you know, I bought the Sprintscan 45. You know that I had first contact you about this and had several inputs from the microscopy listserver. I had a very long delay in getting the thing to me and when it finally arrived, there were parts missing. You had me contact Dennis Lizier and he sent out the standard parts for the scanner (sans cable, but I already had one). However, I told him that you had said that the TEM negative carrier with the antireflection glass plates would also be sent to me. I haven't received it. I have left several messages with him about this, but I have not gotten a response or a call back. The original order went out in Jan. We have paid for the unit with the good faith that the carrier would be delivered. Am I going to receive the negative carrier? Can you please help me? -Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
---------- From: matthias.morgelin-at-medkem.lu.se To: Microscopy-at-sparc5.microscopy.com Subject: negative scanner Date: Friday, June 26, 1998 5:05AM
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we want to buy a scanner which can be used for both 35 mm and 70 mm EM film as well as 81x100 mm plates, probably with different adapters (?). Software must be compatible with Windows 95/NT 4.0 (e.g Adobe, Freehand...). The higher resolution the better (at least 1200 dpi), the cheaper the better, as usual. Any helpful comments or experience? - would be highly appreciated. Thanks a lot,
I'm required to inject some sheep at 2 hourly intervals with Brdu. Most papers refer to a "magic" figure of 5mg/kg body weight, so per injection I'll need 250mg of Brdu. We seem to have a few problems dissolving this quantity of Brdu in a reasonable amount of saline (ie 20mls or less, in 20mls concentration would be 4mol/l). . Any thoughts on improving solubility would be appreciated.
Regards Peter Smith AgResearch Wallaceville Upper Hutt New Zealand
The highest concentration I've been able to acheive in .9% saline is 12 mg/ml. That will ppt. when refrigerated, but goes back into solution thwn warmed and mixed. 10 mg/ml is the highest concentration that doesn't ppt. in the cold, and it mixes more quickly than 12 mg/ml.
Regards, Glen
On Tue, 30 Jun 1998, Smith, Peter wrote:
} I'm required to inject some sheep at 2 hourly intervals with Brdu. Most } papers refer to a "magic" figure of 5mg/kg body weight, so per injection } I'll need 250mg of Brdu. We seem to have a few problems dissolving this } quantity of Brdu in a reasonable amount of saline (ie 20mls or less, in } 20mls concentration would be 4mol/l). . Any thoughts on improving } solubility would be appreciated. } } Regards Peter Smith } AgResearch Wallaceville } Upper Hutt } New Zealand } } smithp-at-agresearch.cri.nz }
____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; AFM Technology Leaders for Environmental / In Vitro AFM 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
In my opinion you should think of: * the system contains free prim antibodies (prim Ab's which have detached from the antigen, or excess prim Ab's which have not been removed be a washing step). The free prim Ab's react with the GAM preventing the GAM to bind with the antigen-IgG-complex. * steric hindrance by a connective tissue component preventing the binding of the GAM to the prim. IgG. * presence of an other reagent for the prim. IgG's in the system binding first and occupying the binding sites for the GAM * incubation conditions which denature (conformational changes) the prim IgG
Check: * confirm the binding of the prim Ab's * incubate the prim Ab's and the GAM to interact with each other in an other incubation environment * perform the reaction using an other batch of GAM
Good Luck,
Marcel
------- Forwarded Message Follows -------
Dear Microscopists:
I am in a mild state of confusion regarding an immuno-EM experiment where it seems that the secondary antibody (GAM 5 nm) does not recognize the primary (mouse IgG) once the primary is bound to its corresponding antigen in tissue. We expect that the antigen is present with periodicity on fibrils in the connective tissue matrix. After the tissue is emersed in antibody, we see periodic decoration of the fibrils, which indicates to us that the antibody is bound. However, secondary antibody does not bind to the tissue. We are convinced that the secondary conjugate is not defective, as we use it for other experiments. Has anyone else experienced a situation where a secondary does not recognize a primary once the primary is bound to its corresponding antigen?
Many thanks for your consideration,
Doug ---------------------- Douglas R. Keene Associate Investigator Shriners Hospital Microscopy Unit Portland, Oregon 97201 DRK-at-shcc.org
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Has anyone ever observed anything similar to this:????If so how did you } fix it??? } } I have a filament image vibration in one direction at 30-60hz in a JEOL } 4000. It is very subtle. I have observed this for 3 years but the } amplitude has increased such that we would like to pin down the source } and correct it. } } Since initially observing the problem we have had a gun change, several } filament changes, the high voltage tank worked on so I do not see these } as probable causes. Also I do not think these would give a one } direction effect. The magnetic fields of computers, monitors, and step } motors do not seem to have any effect on the filament image vibration. } } I am thinking that the deflector circuits would be most suspect. Usual } tests on the power supplies look good. } Any ideas would be appreciated. } -- Hi Roseann,
Do you know where in the microscope the deflection is coming from? Is it in the same direction as one of the defectors (this can be checked)? Where is it in the column? The direction will change, due to the rotation of the lens, if it is above any lens you change. This can be used to locate the position of the instability in the column. I assume that the image is stable so it must be the gun or condensers. Any clue as to where to look will help.
One of our 4000s has an occasional problem that may be associated. Someone around here uses equipment that generates a field which seems to be picked up by the objective stigmator coils (or more likely the amplifier circuits). This affects one 4000 but not the other which is sited in the next room, I wonder if different amplifier circuits may be sensitive to particular frequencies. Have you got any field measuring system? If so can you match the frequency of the deflection to the frequency of an external field, even if it is within specification? Naturally by the time we get round to looking for the cause the equipment has been switched off, but if your problem is constant then you might have a chance.
Good luck Ron =========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
I want to take this time to thank everyone who responded to my recent question regarding preparation of wood composite samples for optical and electro-optic microscopy. It appears that the most viable suggestion is to use cryosectioning -- an option that I intend to vigorously pursue. Your help is appreciated.
Dear all, I am working on goat mammary gland involution. My problem is to find specific antibodies for Collagen IV, Collagenase IV, Upa and other costituents of basement membran, cross-reacting with goat tissues Could anyone help me? Thank you Silvia
Silvia Modina, PhD Institute of Anatomy of Domestic Animals Laboratory of Embryology and Animal Reproduction Faculty of Veterinary Medicine University of Milan Via Trentacoste, 2 20134 Milano, Italy Phone: (+39) 2 21.54.036 Fax: (+39) 2 21.40.745 E-mail: silvia.modina-at-unimi.it
At 12:24 am +0200 1/7/98, Henrik Kaker wrote: } As I know there is no such list server. } } Henrik } } geos-at-goldrush.com wrote: } } } } } } } Does anyone know of the list server for DM? } } } } George
As far as I remember, Digital Micrograph is a Gatan product. Why don't you look up Gatan at http://www.gatan.com/
Hope this helps
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Winnie Westbrook wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Please post this position description. } } #03171-Lab Specialist Senior - CISAT } Assists in all aspects of procedures required for the prepapration of } tissue for immunohistochemical analysis (whole mounts as well as } sections), fluorescent and light microscopy, transmission and/or } scanning electron microscopy. Competitive candidates must possess } knowledge of techniques for immunological staining, sectioning, mounting } tissue, evaluating sections and taking and developing publicaiton } quality photomicrographs. Applicant must possess demonstrated ability } to operate: ultramicrotome, vibratome, cryostat, and other TEM/SEM } equipment. Applicant will be responsible for maintenance of specimen } records, chemical supplies, reagents, etc in the histological laboratory } and for independently modifying lab procedures to obtain optimal } results. Interpretation of findings and ability to direct the work of } others also necessary. Four year degree in Biology or related field } supplemented by post-graduate experience in microbiology and/or } neuroscience preferred. Salary range: $24,337-37,995. } } Send resume and all correspondence to: } } Brenda M. Ryals, Ph.D } Professor } Communication Sciences and Disorders } James Madison University } Harrisonburg, VA 22807 } ryalsbm-at-jmu.edu
To All Who Applied:
All applications are being reviewed. The posting is now closed.
Are you certain that the primary can actually be recognized by the secondary, I mean do they match? You could use a dot spot test to see if they do (described in our Newsletter nr. 4). If the primary is recognized by the GAM-5 in such a test, you may have steric hindrance in your specimen. In that case using smaller particles may solve the problem. You could check for this by using GAM-FITC or the like.
Good luck, Jan
============================= Jan Leunissen, Ph.D. AURION ImmunoGold Reagents & Accessories Managing Director Costerweg 5, 6702 AA Wageningen The Netherlands
Smith, Peter wrote: } } I'm required to inject some sheep at 2 hourly intervals with Brdu. Most } papers refer to a "magic" figure of 5mg/kg body weight, so per injection I'll need 250mg of Brdu. We seem to have a few problems dissolving this quantity of Brdu in a reasonable amount of saline (ie 20mls or less, in 20mls concentration would be 4mol/l). . Any thoughts on improving solubility would be appreciated. } } Regards Peter Smith } AgResearch Wallaceville } Upper Hutt } New Zealand } } smithp-at-agresearch.cri.nz
Peter:
I dissolve 5 mg/ml Brdu without any problem in 0.9% saline containing 0.007N sodium hydroxide. I warm it in my hand for a few minutes. However, this concentration seems to be lower than what you are aiming for.
Geoff -- *************************************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane Piscataway, NJ 08854 voice: (732)-235-4583; fax -4029 e-mail: mcauliff-at-umdnj.edu ***************************************************************
Dear Melany, your asking a lot here. There are so many considerations and variables to discuss in the paraffin vs. plastic question. Both techniques require a great deal of time for proper infiltration of plant tissues so that is not a factor. Ultimately, sections of tissue embedded in plastic (resin) have the potential to look better (ie. be thinner and allow better resolution if fixed properly). Paraffin is the way to go if you have a large project requiring survey type sections of hundreds of flowers or very large block faces as can be the case in a flower development study. 'Fairly' hard tissues can be sectioned in paraffin if the proper softening protocol is followed however palm fruits might be tricky in later stages.
I usually prefer to embed in plastic. There is no reason to use 'resin' (e.g. Spurr's (I don't recommend Epon for plant tissue BTW)) unless you are going to be doing TEM work. Some plastics like JB-4 (I think it's a methacrylate?) are easier to use, are less toxic and can be sectioned in much larger block faces at incredible thickness i.e. } 10 micrometers if you so desire. I have had good results lately sectioning JB-4 embedded flowers down to 1 micrometer thick with an Olympus rotary microtome. This requires however that you can equip your microtome with a glass knife holder. I have tried steel and tungsten knives with plastic and resin embedded tissue with no great success. If you already have a paraffin set-up you will find that most of it isn't used in the plastic embedding protocol and there will be a initial expense for plastic embedding accessories. Also, sectioning of 'hard' tissue may in fact be more difficult after embedding in plastic or at any rate wouldn't be too much easier. Woody tissues are usually a problem for the anatomist and the problem is probably best solved by treatment to soften the tissue prior to embedding in any media.
A few studies of parrafin embedded tissue have been published lately but their numbers are in decline. Some journals and reviewers seem to prefer the higher quality of sections/micrographs that are possible after plastic embedding. Ask yourself these questions as a guideline:
1) How many blocks to be sectioned? 10-100 plastic, } 100 paraffin.
2) Can a glass knife holder be obtained for the Spencer 820 or do you have access to an ultramicrotome? The glass knife limits the block face size to around 1/4 inch wide although see the recent discussion of Ralf Knife making techniques.
3) Can you afford the dollars required to convert to plastic? A example list of supplies that are different from the paraffin procedure would include: glutaraldehyde, buffer chemicals, vacuum pump and dessicator for infiltration, plastic embedding kit, polymerization trays and blocks for JB-4, glass knives and holder for microtome, and lots of sundry items.
Finally, the protocols for plastic and resin embedding are published in many places. Find any good book on plant microtechnique. The ones I always recommend are:
O'Brien, T.P. and McCully, M.E. (1981) The study of plant structure: principles and selected methods. Termarcarphi Pty. Ltd. Melbourne, Australia.ISBN 0 9594174 0 0.
Berlyn, G.P. and J.P. Miksche (1976) Botanical microtechnique and cytochemistry. Iowa State University Press, Ames, Iowa. ISBN 8138 0220 2
Good luck and write to me if you have other questions, John
} I am preparing to make microscope slides of palm flowers and fruits. } Some of the fruits are quite woody as are many of the developmental } stages of the flowers. I had planned to use the old parafin method of } microtome preparation but I have been advised to consider resin. My } funds and resources are limited, but the resin method seems to have more } advantages. } } Questions: } 1. Can I use a manual rotary Spencer 820 American Optical Corp Microtome } on resin samples? } } 2. What are the drawbacks to using the resin method? } } 3. What are the drawbacks to using the parafin method } (toxicity/results/time)? } } Thanks in advance for any and all responces. Please reply to my email: } crow-at-aloha.net } } Best wishes, } } mattie
________________________ C. John Runions, Ph.D. Section of Ecology and Systematics Corson Hall Cornell University Ithaca, New York USA 14853
geos-at-goldrush.com wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Does anyone know of the list server for DM? } } George
} As I know there is no such list server. } } Henrik } } geos-at-goldrush.com wrote: } } } } } } } Does anyone know of the list server for DM? } } } } George
A listserver for users of the DM Scripting Language does exist. Check http://www.public.asu.edu/~perkes/DMSUG.html for details.
Gert
==================================================================== Dr. Gert Oostergetel Phone: +31 50 363 4223 Biophysical Chemistry Fax: +31 50 363 4800 University of Groningen Nijenborgh 4 E-mail: oostergetel-at-chem.rug.nl NL-9747 AG Groningen The Netherlands ====================================================================
High, I'am studing osteoclasts cells culture "in vitro" on dentine slides in an Environmental Scanning Electron (Electrosan 2020). Is there anybody who has been working on this subject ? I need some recomendations (kV, Working distance, Pv H2O), or litaracy on this subject, because I didn't find anything. Thank you very much.
Dear all, does anyone know of a graphics package that can take several separate digital image files and montage them on a single page ready for printing?
Thanks,
Mark Munro
From Microscopy-request-at-MSA.Microscopy.Com Tue Jun 30 16:16:15 1998 Return-Path: Microscopy-request-at-MSA.Microscopy.Com Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id NAA22529 for dist-Microscopy; Tue, 30 Jun 1998 13:03:31 -0500 Received: from Pegasus.cc.ucf.edu (Pegasus.cc.ucf.edu [132.170.240.30]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id NAA22523 for {microscopy-at-Sparc5.Microscopy.Com} ; Tue, 30 Jun 1998 13:03:03 -0500 Received: from port14.3a.tserver.ucf.edu ([132.170.24.144]:2048 "EHLO [132.170.24.144]" ident: "NO-IDENT-SERVICE") by pegasus.cc.ucf.edu with ESMTP id {20503-14402} ; Tue, 30 Jun 1998 13:59:54 -0400 Message-Id: {l03130303b1bed89d6d25-at-[132.170.24.144]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
There is no need to switch from Freon to SF6. We were able to get 84 lbs of Freon for ~$1000. Freon is readily available if you can show that you are not using it as a refrigerant.
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-pegasus.cc.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
There is no need to switch from Freon to SF6. We were able to get 84 lbs of Freon for ~$1000. Freon is readily available if you can show that you are not using it as a refrigerant.
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-pegasus.cc.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
Hello, everyone. Last week I asked for information about breaking glass Histo knives, and we are following up on the "freehand method(s)" recommended, but we have also been VERY fortunate enough to receive an LKB 2078 Histo Knife Maker as well!
Small problem: we do not have any instructions for the LKB 2078, and after having trashed a bunch of glass trying to 'figure it out' (obviously unsuccessfully), does anyone have a set of insturctions they could share with us? A fax would be great, or if you remember your own instructions a step wise summary (its doesn't look that complicated) would also be great.
Thanks!
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
Dear Roeseann, I have seen something similar in my 200 kV Hitachi. It went away when I did a thorough cleaning of the upper accelerator rings. A tiny bit of charging something was causing the rapid deflection of the filament. I never saw the speck that did it, but after the cleaning hte filament was steady. You wrote:
} } Has anyone ever observed anything similar to this:????If so how did you } fix it??? } } I have a filament image vibration in one direction at 30-60hz in a JEOL } 4000. It is very subtle. I have observed this for 3 years but the } amplitude has increased such that we would like to pin down the source } and correct it. } } Since initially observing the problem we have had a gun change, several } filament changes, the high voltage tank worked on so I do not see these } as probable causes. Also I do not think these would give a one } direction effect. The magnetic fields of computers, monitors, and step } motors do not seem to have any effect on the filament image vibration. } } I am thinking that the deflector circuits would be most suspect. Usual } tests on the power supplies look good. } Any ideas would be appreciated. } -- } Dr. Roseann Csencsits
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca
In response to the positive feed back from the dealers of Radical quality products in more then twenty countries. We have decided to open an office in Canada in order to offer wide range of Microscopes, Projectors, OHPs, Educational Laboratory Equipment and Accessories, to North America.
Recognize with various awards for growth and quality in International market at a very competitive prices, we also design and supply custom-built optical system or accessory for any specific need.
I look forward to hearing from you, for any further details on catalogue/pricelist or demonstration of our products.
Hi, I use IP Lab Spectrum to montage micrographs togather. It's a commercial software from Scanalytics. I have used Photoshop but it's slower.
Bob Underwood Derm Imageing Center U of Wash.
On Tue, 30 Jun 1998 mark_munro-at-bio-rad.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear all, } does anyone know of a graphics package that can take several separate } digital image files and montage them on a single page ready for printing? } } Thanks, } } } Mark Munro } }
My research subject are sterile anthers of soyabean. I am mostly doing transmission electron micrographs, and am mostly interested in the tapetum cells during meiosis stages. While I was looking around in the tapetum I came across a nucleolus from which small discreet amounts of some material seemed to emerge (emerge rather then enter, because of the shape of the material). The material seemingly coming out of the nucleolus resembled cloud puff balls or smoke signals from a chimney. Has anybody ever seen such thing and/or knows even what it is???? mRNA, protein, else?
Tracey M. Pepper 1 Bessey Hall Bessey Microscopy Facility Iowa State University Ames, IA 50011 Phone: 515.294.3872 FAX: 515.294.1337 email: tpepper-at-iastate.edu
I posted this question for a graduate student currently working in my facility. Any input on this one??
Anybody working on nucleoli?
My research subject are sterile anthers of soyabean. I am mostly doing transmission electron micrographs, and am mostly interested in the tapetum cells during meiosis stages. While I was looking around in the tapetum I came across a nucleolus from which small discreet amounts of some material seemed to emerge (emerge rather then enter, because of the shape of the material). The material seemingly coming out of the nucleolus resembled cloud puff balls or smoke signals from a chimney. Has anybody ever seen such thing and/or knows even what it is???? mRNA, protein, else?
Tracey M. Pepper 1 Bessey Hall Bessey Microscopy Facility Iowa State University Ames, IA 50011 Phone: 515.294.3872 FAX: 515.294.1337 email: tpepper-at-iastate.edu
Silvia Modina wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear all, } I am working on goat mammary gland involution. } My problem is to find specific antibodies for Collagen IV, Collagenase IV, } Upa and other costituents of basement membran, cross-reacting with goat } tissues } Could anyone help me? } Thank you } Silvia } } Silvia Modina, PhD } Institute of Anatomy of Domestic Animals } Laboratory of Embryology and Animal Reproduction } Faculty of Veterinary Medicine } University of Milan } Via Trentacoste, 2 } 20134 Milano, Italy } Phone: (+39) 2 21.54.036 } Fax: (+39) 2 21.40.745 } E-mail: silvia.modina-at-unimi.it
Try the Devlopmental Studies Hybridoma Bank at http://www.uiowa.edu/~dshbwww or speak with Karen Jensen (319) 335 3826. She is extremely knowledgeable and helpful.
Ronnie Houston Cytochemistry & Molecular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219
____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; AFM Technology Leaders for Environmental / In Vitro AFM 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
Compix software can montage the images to a vertical or horizontal strip or a best fit rectangle.
Patty Jansma Tel:520-621-6671 plj-at-manduca.neurobio.arizona.edu Arizona Research Labs Division of Neurobiology University of Arizona
On Tue, 30 Jun 1998 mark_munro-at-bio-rad.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear all, } does anyone know of a graphics package that can take several separate } digital image files and montage them on a single page ready for printing? } } Thanks, } } } Mark Munro }
____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; AFM Technology Leaders for Environmental / In Vitro AFM 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; AFM Technology Leaders for Environmental / In Vitro AFM 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
I am in a mild state of confusion regarding an immuno-EM experiment where it seems that the secondary antibody (GAM 5 nm) does not recognize the primary (mouse IgG) once the primary is bound to its corresponding antigen in tissue. We expect that the antigen is present with periodicity on fibrils in the connective tissue matrix. After the tissue is emersed in antibody, we see periodic decoration of the fibrils, which indicates to us that the antibody is bound. However, secondary antibody does not bind to the tissue. We are convinced that the secondary conjugate is not defective, as we use it for other experiments. Has anyone else experienced a situation where a secondary does not recognize a primary once the primary is bound to its corresponding antigen?
The problem is not that the secondary does not recognize your primary. It is the fact that colloidal gold reagents can't penetrate into tissues or cells. The gold particles are too large. These reagents are used for labeling surfaces of sections. If you want to use a gold conjugate for whole or thick tissues sections, prior to embedding, you have to use a nanogold reagent, not colloidal gold.
What do you mean that, "After the tissue is emersed in antibody, we see periodic decoration of the fibrils. How do you visualize this?
Joseph Goodhouse Confocal / EM Core Lab Manager Department of Molecular Biology Princeton University jgoodhose-at-molbio.princeton.edu 609-258-5432 Info and Images at http://www.molbio.princeton.edu/confocal/CF-EM-HOME.html
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