I just have to react to your message posted on the Microscopy Listserver as a reply to Doug Keene's question. I fully agree with you that limited or lack of penetration into the section interior may very well be the cause for negative results. I had a message from Doug where he indicates that he will be looking into this, using FITC labels for LM first and ultra small colloidal gold labels with silver enhancement for EM if the first approach proves to be successful. In your answer you say that "you have to use a nanogold reagent, not colloidal gold". As the principal inventors of ultra small colloidal gold particles we demonstrated already back in 1988 that conjugates based on ultra small colloidal particles are very well able to penetrate under circumstances where 5 or 10 nm particle based conjugates will not do so. If you like I will be happy to send you the documentation. The reagents you refer to didn't even exist at that time, although undecagold was known and succesfully used for high resolution immunoelectron microscopy without silver enhancement. The colloidal ultra small labels (sold by several companies with a reputation in gold labels) as well as gold clusters may solve the problem.
Jan
============================= Jan Leunissen, Ph.D. AURION ImmunoGold Reagents & Accessories Managing Director Costerweg 5, 6702 AA Wageningen The Netherlands
AutoMatch is a public domain macro written for the public domain software NIH Image. It is described in an article by Swidbert R. Ott, Microscopy Research and Technique 38:335-339(1997). The montage is automated. The article indicates that the software is available via FTP from zippy.nimh.nih.gov/pub/nih-image/contrib/
Mark, try the imtools package from the SDSC group. Specifically, it contains a program alled imstoryboard that allows you to make a montage of many images into a single file ready for printing. You have control over spacing between images, between images and the edges, the background color, etc. Another way would be to use the netpbm package. Using pnmcat one can concatenate multiple images into a single image. As this one works with standard in and output, quite possibly one could simply redirect the output of it straight to the printer, as in:
pnmcat pnmfile1 pnmfile2 ... | lp (or lpr)
Hope this helps.
Jaap
-- Jaap Brink, Ph.D. Biochemistry, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030 Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu URL : http://ncmi.bioch.bcm.tmc.edu/~brink
On Tue, 30 Jun 1998 mark_munro-at-bio-rad.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear all, } does anyone know of a graphics package that can take several separate } digital image files and montage them on a single page ready for printing? } } Thanks, } } } Mark Munro }
We have the same software and might be able to help with some questions, at least on the image processing side.
Remember, the imaging part is Noesis Vision's Visilog product rolled into the Oxford suite of products.
If anyone would be interested in discussing either Visilog, or ImQuant in particular, I would be willing to set up a mailing list for discussion. Please contact me directly.
At 03:25 PM 6/30/98 +0000, you wrote: } } Has anyone got some tutorials for this Image software. The manual is } too complicated... } } F. (frank.sarrazit)
For calibrating our Mettler FP82HT hot stage, we have been using the melting points of organic compounds supplied with the Kofler Hotbench (Eichsubstanzen fuer Kofler Heizbank). These are now several years old, and someone has suggested that liquid crystals might be more apporopriate.
I would be glad if anyone can update me as to what is state-of-the-art in calibrating hotstages.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } Doug Keene wrote, } } Dear Microscopists: } } I am in a mild state of confusion regarding an immuno-EM } experiment where it seems that the secondary antibody (GAM } 5 nm) does not recognize the primary (mouse IgG) once the } primary is bound to its corresponding antigen in tissue. } We expect that the antigen is present with periodicity on } fibrils in the connective tissue matrix. After the tissue } is emersed in antibody, we see periodic decoration of the } fibrils, which indicates to us that the antibody is bound. } However, secondary antibody does not bind to the tissue. We } are convinced that the secondary conjugate is not } defective, as we use it for other experiments. Has anyone } else experienced a situation where a secondary does not } recognize a primary once the primary is bound to its } corresponding antigen? } } The problem is not that the secondary does not recognize your } primary. It is the fact that colloidal gold reagents can't penetrate } into tissues or cells. The gold particles are too large. These } reagents are used for labeling surfaces of sections. If you want to use } a gold conjugate for whole or thick tissues sections, prior to } embedding, you have to use a nanogold reagent, not colloidal gold. } } What do you mean that, "After the tissue is emersed in } antibody, we see periodic decoration of the fibrils. How do you } visualize this? } } Joseph Goodhouse } Confocal / EM Core Lab Manager } Department of Molecular Biology } Princeton University } jgoodhose-at-molbio.princeton.edu } 609-258-5432 } Info and Images at } http://www.molbio.princeton.edu/confocal/CF-EM-HOME.html
Margaret Springett e-mail hukee.margaret-at-mayo.edu IEM Specialist at Mayo Foundation 1426 Guggenheim Rochester, Mn. 55905
We are getting good results with the Polaroid scanner which is really the Microtek scanner in a Polaroid box. It is not as good resolution for 35mm as the Sprintscan, but in some respects it is better in that a whole bunch of frames can be digitized at once. But as for NT compatibility, we have not tried it on any of the NT machines so we don't know.
-------------------------------------------- Michael Cammer email sent from an account of the Analytical Imaging Facility The Albert Einstein College of Medicine of Yeshiva University 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 FAX: (718) 430-8996 http://leper1.ca.aecom.yu.edu/aif/ --------------------------------------------
On Fri, 26 Jun 1998, Joiner Cartwright, Jr. wrote: } } we want to buy a scanner which can be used for both 35 mm and 70 mm EM } } film as well as 81x100 mm plates, probably with different adapters (?).
Our imaging database, ElectroImages, can montage images easily and with great flexibility. ElectroImages also allows the user to place text captions under images. Captions can include title, date, source, extensive descriptions, filename, filepath, and up to 6 user-definable fields. You can also easily print a footer and header to give your page a finished look.
Please contact me off-list and I will send you our trial copy of this program.
Matt Irwin ElectroImage, Inc. 277 Northern Blvd. Suite 101 Great Neck, NY 11021
LEO Electron Microscopy is hiring SEM service people in California. If you are a proven SEM service/support person, and would like to practise your art for LEO in the SF or LA areas, please contact me direct (billneill-at-csi.com)
} Experienced Electron Microscopist } } A prominent cardiac muscle physiology laboratory at the } University of Pennsylvania has an opening for an experienced electron } microscopist. The individual must be proficient in all aspects of } conventional transmission EM from preparation of the samples to } publication } quality photography. Knowledge of some standard biochemical } procedures } including electrophoresis would be helpful but not essential. We are } looking for an individual who is willing and can demonstrate the } ability to } learn and execute new techniques. A B.S. degree is highly desirable } but not } absolutely essential. Salary will be commensurate with experience. } Please } contact Dr. Saul Winegrad. } } E-mail address is bsg-at-mail.med.upenn.edu } } } } }
I am looking for an instruction of sufficient detection of apoptotic cells in paraplast embedded tissue. Is anybody able to point me to a reference with an adequate recipe or can mail me one?
-- Mit freundlichen Gruessen Yours sincerely *************************************************************** * T. J. Filler | * * Westfalian Wilhelms-Univ.| phone:*49 251 83 552-26 Fax: -41 * * Institute of Anatomy | e-Mail: filler-at-uni-muenster.de * * Vesaliusweg 2-4 | D-48149 Muenster Germany * ****** http://medweb.uni-muenster.de/institute/anat ***********
Warren wrote: "Offhand, I would be suspicious of the cooling fan/system. I have seen a number of computers get flaky once the processor overheated. My first experience of that type was after installing a 486-50 overdrive processor in a PS/1. (Nobody told me it needed a cooling fan.) Things come to a halt very quickly once the processor overheats, and it doesn't take all that long to warm up. 5-10 minutes sounds like plenty long enough. I have had the same experience with trying to drive a processor a little to hard, like when I was trying to push my 120 MHz up to 133 MHz. It didn't seem like much of a push, but the manufacturers have already well pushed to the edge so that there is no room for a cooling fan to fail"
But the real truth is that CPUs are rated extremely conservatively. Typically, the maximum speed diminishes as they age, and no responsible manufacturer wants to risk having to replace millions of microprocessors on warranty. That is why there can be considerable ability to "hot rod" CPUs, and still keep them running-for a while. Today's CPUs have the clock speed burned in at the factory, and will be reliable for very long periods of time.
-----Message d'origine----- De : Lucille A. Giannuzzi {lag-at-pegasus.cc.ucf.edu} =C0 : microscopy listserver {microscopy-at-sparc5.microscopy.com} Date : mercredi 1 juillet 1998 05:10 Objet : SF6 upgrades for JEOL TEMs
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear Lucille
The new norm about CFC's regulation don't take care of the use you'll mak= e of freon. The important thing is to not let freon going to the atmosphere and you cannot be sure of that during emergency on the gun or on the HT tank. This pollution is too bad and freon will not be available soon. I think your provider has to sell his stock which we will not be abble to u= se later.
I received a message from a coworker asking if I had any information to help you.
I am sending you a web site that deals with old scientific instruments and of course includes microscopes. I purchased two book from them, which might help:
1. Turner, G.L'E. COLLECTING MICROSCOPES $25.00 Christie's International Collectors Series New York: Mayflower Books, 1981 8vo, pp 120; cloth, dj; new copies (out-of-print) with 102 instruments illustrated, many in color
____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; AFM Technology Leaders for Environmental / In Vitro AFM 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
The Adem was ahead of it's time. It has a six axis stage, fully integrated imaging, and X-ray analysis. The column can accept very large samples, and it has a motor for everything. They were coming out with a FE model when they pulled the plug back in the early 90's. This was due to several factors. The 5500 series 2 X-ray system was nearing the end of it's sales cycle, and the other Microscope vendors did not take too kindly to the competition. This caused sales alliances with the OEM's to collapse. Throw in the fact that Noran was bought and sold twice in that time frame. So the new management felt they had to save the core business. There were still some working units out there when I left Noran 2 years ago.I worked on them briefly and I found them to be difficult to work on. The concept of a fully integrated SEM was sound, however, they made it way too complicated. If Noran could have held on for a while longer they would probably have a good market share. I found it interesting that the chief designer Fred Schamber went to R J Lee and designed the personal SEM. The same concept only on a much simpler scale.
Thu, 2 Jul 1998 09:25:13 +0100 Received: from localhost by spsscsc2.rdg.ac.uk (8.8.5/8.8.5) with SMTP id JAA24200; Thu, 2 Jul 1998 09:25:00 +0100 (BST)
On Tue, 30 Jun 1998 mark_munro-at-bio-rad.com wrote:
} Dear all, } does anyone know of a graphics package that can take several separate } digital image files and montage them on a single page ready for printing?
For a GENERALLY USEFUL package which can do things in a nice way, try downloading a trial version of Corel Xara! 2.0 from:
http://xaraxone.i-us.com/
I have only recently acquired this, so I haven't tried out all its functions, but I have started converting some of my black-and-white TEM images to duotone using this package.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
If you want to impress the kiddies, go ahead and use those liquid crystals. After all, it sounds much fancier that way.
But I certainly wouldn't bother. There is nothing at all wrong with regular melting point standard substances. And viewing in cross polarizers, the melting temperature is always quite clear. I certainly wouldn't buy anything new if I were sure that the existing materials were OK. (If they're not, the melting point is no longer sharp. You can test this in a DSC.)
Was the person suggesting other standards in any chance a salesman wanting to sell them to you?
Cynthia Bennett Hoechst Diafoil
The opinions expressed here are solely my own. Don't blame my employer.
------------------------------------------------------------------------ - Robert Olley wrote:
For calibrating our Mettler FP82HT hot stage, we have been using the melting points of organic compounds supplied with the Kofler Hotbench (Eichsubstanzen fuer Kofler Heizbank). These are now several years old, and someone has suggested that liquid crystals might be more apporopriate.
I would be glad if anyone can update me as to what is state-of-the-art in calibrating hotstages.
+----------------------------------------------------------------------- -+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley |
There will be a meeting of the MSA Standards Committee and ASTM committees E42-96 (US TAG for ISO TC202 Microbeam Analysis) and E42-15 (Electron Probe Microanalysis/Electron Microscopy) on Thursday July 16th at 12:30 PM in Rm. 275-W of the Georgia World Congress Center. For additional information contact john.small-at-nist.gov.
Hi! Does anybody know if there is any problems (eg precipitate within the tissue, etc) if tissue is fixed in 2% osmium tetroxide within a stainless steel container? Thanks Dorota
Everyone was so helpful when I was looking for a large SEM chamber. Can you help again?
I had a call from a science museum in Virginia. They are setting up a display and would like an illustration of the crystallographic structure of aluminum and if possible, a 7079 alloy.
I have at my ready grasp a drawing of graphite, which is close. But museums tend to be very particular and they should have aluminum. They would like the drawing for reference in constructing a model.
If you have such a drawing, please contact Mr. Brian Alfano at balfano-at-smv.mus.va.us
We have funding to buy a new SEM and have at least one application for an = environmental(variable pressure) microscope. Those of you who have = experience with environmental scopes, especially the Hitachi 3500N, please = let me know the good and bad points about your scope. Most of our samples are animal tissue and some of my reading has = suggested that to prevent tissue drying, when working at low vacuum, it is = necessary to use a cold stage. How do you rate the importance of a cold = stage for this type of specimen? =20 Finally, those of you who have a cold substage from Fullam... have you = been satisfied with its performance? =20 Any comments are welcomed. Thanks.
Donna Wagahoff SIU School of Medicine PO Box 19230 Springfield, Il 62794-1220 217-782-0898
Here's a general question that should stir up some comment...
My department has sent out a standard questionaire for us to estimate any problems our lab computers, software, or equipment might have with the year 2000 date problem. Perhaps some of you have already gone through this. I'm not a computer wiz, and naively thought that everything I use would be fine, since I don't do any date calculations like folks in the banking, insurance, etc. businesses do. However, the more I hear about this problem the more confused I get. Is it true that some older CPU's or BIOS programs will not handle the "00" date? So some of our older equipment might not work even though the software we are aware of has little reliance on dates?? Any microscope or software vendors want to comment??
Hope this isn't stirring up needless fears or urban legends, but I have definitely heard some alarmist opinions and would like to consult a more technically aware and level-headed source (i.e. yourselves!).
Thanks, Karen
-- Karen Zaruba, kszaruba-at-mmm.com BioMaterials Technology Center 3M Center Bldg. 270-1S-01 St. Paul, MN 55144
*The opinions above are my own, not necessarily my employer's*
I wish to inject ferritin into the hemolymph (blood) of crabs to determine which spaces are blood spaces. (Crab blood has copper-based hemocyanin, rather than hemoglobin).
Sigma carries several varieties of Ferritin, including Apoferritin. Any suggestions as to which one I should use?
Does anyone have a protocol to recommend?
What final concentration should I shoot for in the blood stream?
Thanks to all respondents.
______________________________________________________________________ Donald L. Lovett e-mail: lovett-at-tcnj.edu Assoc. Professor, Dept. of Biology voice: (609) 771-2876 P.O. Box 7718 fax: (609) 637-5118 The College of New Jersey Ewing, NJ 08628-0718
A recent breakthrough in environmental microscopy technology is MAC Mode-AFM. This will allow you to do in vitro, time lapse imaging at a nanometer scale resolution.
You can use a 37 C temperature control with flow through liquid cell to change your biological buffers and observe conformational change at the molecular level.
Also you can characterize attractive and repulsive forces between antibody - antigen or other bio agents. A great paper, "Antibody-Antigen Recognition Detected By Ultra sensitive Force Microscopy" will be presented at Protein 98 san Diego by Peter Hinterdofer of the Schindler group from Linz Austria.
George
At 05:55 PM 7/2/98 -0600, Donna Wagahoff wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Does anyone have experience with e.g. marine samples in ESEM? Or tissue in buffers? I am curious to know whether they are feasible. With samples, such as small planktonic organisms which are living in what is essentially 3% sodium chloride solution, can they be dried off without getting a coating of salt crystals? I know from cryoSEM that once the water is removed, you get the covering of salts. I imagine that some items might withstand a quick rinse in fresh water, but not everything.
Karen, Most old PCs (but not Macs or most Unix) will not transfer the date correctly to the year 2000 although many will handle dates after Jan 1, 2000 if told explicitly (i.e. with 4 figure years). You can get free test software for PCs off the www. I found one from National Software Testing Laboratories (NSTL) that seemed pretty useful. If your old PC will not do the transition automatically you can either roll the date forward manually (if it can do this), use a false date (such as 1990) but obviously this could be misleading in some cases, or scrap the PC and buy a new one.
For the most of our PCs that control lab equipment in our group I know that they will not roll the date over automatically, so I am likely to keep all the data backed up, wait till the end of 1999 and see what happens. If one dies for some reason then we can always replace it with another cheap PC.
Hope this helps
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
This is a multi-part message in MIME format. --------------99D16DAA7889B787EDE9016F Content-Type: text/plain; charset=big5 Content-Transfer-Encoding: 7bit
A postdoctoral position in scanning tunneling microscope investigation on the interfacial reactions of metal thin films on silicon is available at the
IC Thin Film Lab. Department of Materials Science and Engineering National Tsing Hua University Hsinchu, Taiwan, Republic of China
The position is for one year and renewable for two more years. Interested persons should have experiences in UHV-STM. Please contact Professor Lih J. Chen directly either by e-mail or by fax at 886-3-5718328.
--------------99D16DAA7889B787EDE9016F Content-Type: text/x-vcard; charset=big5; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Lih J. Chen Content-Disposition: attachment; filename="vcard.vcf"
begin: vcard fn: Lih J. Chen n: Chen;Lih J. org: Department of Materials Science and Engineering, National Tsing Hua University adr: Department of Materials Science, National Tsing Hua University;;;Hsinchu;Taiwan;300;Republic of China email;internet: ljchen-at-mse.nthu.edu.tw title: Professor tel;work: 886-3-5731166 tel;fax: 886-3-5718328 x-mozilla-cpt: ;0 x-mozilla-html: FALSE version: 2.1 end: vcard
The question has been posed whether cells grown in culture or cells from sea water can be imaged using ESEM. As a general principle I have found it possible to image cells in culture medium - crystallisation upon drying. It is possible to image cells from 0.9% saline and in such cases the NaCl crystals are widely dispersed and mostly do not interfere with imaging (sod's law not withstanding!) I can try 3% saline to see what happens and will let you know.
Another question needs to be addressed however. What are you trying to see? I have much success by viewing glutaraldehyde fixed samples washed in water and imaged with ESEM. The cells remain hydrated are much more robust (resistant to accidental drying, withstanding beam damage). On most occasions I have not seen structural differences between fresh hydrated and fixed hydrated. It is the drying which causes changes not fixation.
As with all SEM low kV should be used (hard work below 5KV unless you have Brendan Griffin detector modification). Check the pressure temperature curve for water vapour pressure but 3 - 4 degrees C with a pressure of 5 - 6 torr should keep specimens hydrated.
Good luck Let me know if I can help further.
Chris
Chris Gilpin Biological Sciences Electron Microscope Unit G452 Stopford Building Oxford Road Manchester M13 9PT phone +44 161 275 5170 fax +44 161 275 5171 http://www.biomed.man.ac.uk/biology/emunit/emhome.html
If you are attending the MSA Meeting in Atlanta, Mike Boykin and I would like to invite you to a gay social on Monday, July 13th at 8:00pm. The party will be held at Mike's home in midtown Atlanta. Directions to Mike's house will be available via email or at the Information and Hospitality Booth. Also, copies of Southern Voice (a gay weekly newspaper) will be available. If you are planning on attending the social please RSVP to me. See y'all there, Beth Richardson
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
For those interested, I thought I would summarize the comments received regarding my last querry to the listserver, which was:
Dear Microscopists,
I am in a mild state of confusion regarding an immuno-EM experiment where it seems that the secondary antibody (GAM 5 nm) does not recognize the primary (mouse IgG) once the primary is bound to its corresponding antigen in tissue. We expect that the antigen is present with periodicity on fibrils in the connective tissue matrix. After the tissue is emersed in antibody, we see periodic decoration of the fibrils, which indicates to us that the antibody is bound.
However, secondary antibody does not bind to the tissue. We are convinced that the secondary conjugate is not defective, as we use it for other experiments. Has anyone else experienced a situation where a secondary does not recognize a primary once the primary is bound to its corresponding antigen?
Responses:
Several responses pointed out that secondary antibody conjugates are sub-type specific, meaning that if my primary was really an IgM and not an IgG, that a GAM IgG would not recogize the IgM. In my case, I know that the primary is an IgG.
Several responses suggested that I might not reasonably expect primary and secondaries to penetrate into the connective tissue matrix, that it might be to dense. Therefore I should consider a surface labeling procedure during which the antigen is exposed via sectioning. Unfortunately, this antibody will not work in surface labeling procedures, even though I have tried many different fixation/embedding techniques. Also, we have had years of success diffusing antibody into the CT space, including antibodies to other components of this same microfibril. However, as pointed out by another response, the gold particule we are using (5 nm) may be to big, and steric hindrance might be an issue. The suggestion is to try LM first, with a smaller FITC conjugate, then if positive use a smaller (1 nm) secondary for EM. This is the procedure we are about to try.
Many people still call this meeting MSA, but I do believe that the meeting is sponsored by the Microbeam Analysis Society and the Microscopy Society of America and that is why it is known as Microcopy and Microanalysis 98. There seems to be a general lack of understanding that the meeting is a joint affair of the two socities.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
This sounds more probable with Environmental SPM imaging in the 3% sodium chloride solution / buffer. There is not need to dry it of and you can image in vitro.
George
At 08:16 AM 7/3/98 +0100, Keith Ryan wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear Donald, } } I wish to inject ferritin into the hemolymph (blood) of crabs to determine } which spaces are blood spaces. (Crab blood has copper-based hemocyanin, } rather than hemoglobin). } } Sigma carries several varieties of Ferritin, including Apoferritin. Any } suggestions as to which one I should use? } I think the apoferritin is the protein without the iron; if so, it would be a very poor tracer.
} Does anyone have a protocol to recommend? } If you can map the iron, you can see where it appears and where it is co-localized with copper. If you do not have element-mapping capability, the problem is much more difficult. In the latter case, some gold-labelled anti-ferritin (or forgetting the ferritin altogether, gold-labelled anti- hemocyanin) could work.
} What final concentration should I shoot for in the blood stream? } For EDS you would need a reasonable fraction of 1% iron; for WDS you need much less--I'll let the WDS experts tackle this one. Good luck. Yours, Bill Tivol
MSA's middle school microscpoy manual has JUST been published! Here's a brief description (taken from the Project MICRO bibliography):
Brady, S. and Willard, C. 1998 Microscopic Explorations 158pp, paperback, 8.5x11". ISBN 0-924886-00-5 Lawrence Hall of Science, University of California, Berkeley, CA 94720-5200; 510-642-7771 or 7262, gems-at-uclink.berkeley.edu. A collaboration between the Microscopy Society of America and the LHS has produced an outstanding Great Explorations in Math and Science (GEMS) guide. It's written in "festival" format, with a dozen explorations that can be presented simultaneously to circulating groups of students. There is a rich assortment of supplemental information on microscopes and how to buy them, curriculum extensions, further reading, and sources of help. The units are more classic than unique; subjects include crystals, color printing, fingerprints, pond water, brine shrimp, etc. Its uniqueness lies in the carefully written "inquiry science" presentation of those topics and the thorough classroom testing of content that a GEMS guide receives. It will work well in any classroom; teachers aren't expected to have special skills. Information on other GEMS guides (there are over 50!) is available at www.lhs.berkeley.edu/GEMS. Grades 4-8.
The retail price is $21.00, plus $4.00 shipping (in the U.S.); MSA members may request a 15% discount. A limited number of copies will be available at the Project MICRO booth in Atlanta. See y'all there!
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
I've put some of the material from our LASER diffraction workshop on the web*, so as to share the fun. This began as a 2.5 hour workshop for gifted high school students participating in a six week program (the "Engleman Institute") here at UM-StL. A bit of thought, however, has suggested that even some of our diffraction experts might enjoy both the subjects, and the competition.
I would encourage microscopists to check out there computers with regard to the Y2K problem. However, I expect that the impact will be minimal, if not trivial, in practically all cases. We don't have the same sort of issues to deal with as the financial and insurance people.
I just checked out our computers using the test program from NSTL described by Ian below (the software is available from http://www.nstl.com/html/y2k_faq.html). It seems to do a good job of finding the problems, if any.
Our new computers (Pentiums and newer) checked out okay.
Our two remaining 486 machines reported minimal problems. The date would not correctly rollover on 1-1-2000. If the computer was powered off and on after 1-1-2000, the system would come up with a date of 1980. However, if the DOS DATE command was given on or after 1-1-2000 and the system restarted, it would come up with a correct date. There is a "century bit" in the BIOS info somewhere that will stay set once set, but does not appear to roll over correctly on these old machines.
Therefore, we can simply use the DOS DATE command after 1-1-2000 and reboot. Or I found that a utility form RightTime (www.RightTime.com) called YEAR2000.COM will fix the BIOS so that the computer passes the NSTL test. I do not know if it needs to be kept loaded after 1-1-2000. I would definitely want to check before I removed it.
The Year2000.txt file that comes with the RightTime utility does a very good job describing what the technical issues are for those who care to look deeper.
BIOS fixes are available for many computers to fix the problem. However, they may not be woth the hassle unless they are needed to fix another problem.
As far as application problems, I cannot imagine much happening in microscopy programs. If programs use only a two character year code, the code should still get transferred correctly. The problems will probably crop up when sorting or searching according to date. I have noticed the following behavior with some of our programs.
Access 7.0 (for 95) - Entering a value of 1/1/00 will be interpreted as 1900. You can and must enter 2000 (four digits) as the year for it to register as the next century. Otherwise 00 and 01 will precede 98 and 99 when sorted in ascending order.
Excel 95 - Entering 2-digit dates 00 through 19 is treated as 2001 to 2019. (You can check by formating cells to show the full 4-digit date.) 2-digit dates from 20 through 99 are treated as 1920 through 1999. In other words, their two digit century runs from 1920 through 2019.
Paradox database (as used by Quartz PCI, and others) - A search for dates after 1/1/30 return no files, while a search for files before 12/31/29 returnes all files. Their two digit century apparently is defined to cover the years 1930 through 2029.
To Summarize: A - Nothing broke in a real bad way. Even if something did, I suppose that 1999 could be extended artifically by backdating the computer until a suitable fix was found.
B - Many 486 and earlier computers will probably need some attention, but can probably get by with manual use of the DATE command after 1/1/2000. BIOS fixes are available for many computers if someone wants to go that route.
C - The data may have the correct two digit year code, but you may have to be wary how you search or sort the data. As long as manufacturers have written their code so that the two digit century encompasses the full range of our data collection, we should be all right. And since I don't have any data files collected between 1920 and 1929, I think I can safely assume that two digit codes representing the years 1930 through 2019 for my files will be treated correctly.
Hoping this helps, Warren Straszheim
At 10:26 AM 7/3/98 +0100, Ian Mc Laren wrote: } Karen, } Most old PCs (but not Macs or most Unix) will not transfer the date } correctly to the year 2000 although many will handle dates after Jan 1, } 2000 if told explicitly (i.e. with 4 figure years). You can get free test } software for PCs off the www. I found one from National Software Testing } Laboratories (NSTL) that seemed pretty useful. If your old PC will not do } the transition automatically you can either roll the date forward manually } (if it can do this), use a false date (such as 1990) but obviously this } could be misleading in some cases, or scrap the PC and buy a new one. } } For the most of our PCs that control lab equipment in our group I know that } they will not roll the date over automatically, so I am likely to keep all } the data backed up, wait till the end of 1999 and see what happens. If one } dies for some reason then we can always replace it with another cheap PC.
I am looking for PTFE tubing to fit a Hexland cryostage. Does anyone know who carries parts for this unit?
Thanks, Carl
====================================== Carl Henderson Electron Microbeam Analysis Laboratory University of Michigan 2501 C.C. Little Bldg. Ann Arbor, MI 48109-1063 USA (734) 936-1550 FAX (734) 763-4690 ======================================
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Hi Donna, Hope all goes well with you. Haven't talked to you ikn a long time. ESEM There is actually only one true environmental scanning EM and that is the = Philips Electroscan. The advantage is that samples are kept humid and do = not dry out. Any other "Vairiable pressure" SEM dries out the sample = with only short times to look at it. SO, as everything else, it depends = on what you want to do with the scope. If you are really looking at wet = samples, then the true environmental SEM seems appropriate. If that = isn't of interest then the others are cheaper. My two cents, Judy M.
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
We have funding to buy a new SEM and have at least one application for an = environmental(variable pressure) microscope. Those of you who have = experience with environmental scopes, especially the Hitachi 3500N, please= let me know the good and bad points about your scope. Most of our samples are animal tissue and some of my reading has = suggested that to prevent tissue drying, when working at low vacuum, it = is necessary to use a cold stage. How do you rate the importance of a = cold stage for this type of specimen? Finally, those of you who have a cold substage from Fullam... have you = been satisfied with its performance? Any comments are welcomed. Thanks.
Donna Wagahoff SIU School of Medicine PO Box 19230 Springfield, Il 62794-1220 217-782-0898
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Dear List,
We recently purchased a microwave system for tissue fixation/embedding. Unfortunately, the instruction book that we ordered on it's use did not come and we now find out that it is no longer available (through Ted Pella). Does anyone have any information on procotols or know of any good reference books on microwave fixation and embedding? Any information that will get us going would be greatly appreciated.
William R.McManus Supervisor Electron Microscope Facility Department of Biology Logan UT 84322-5305 billEMac-at-cc.usu.edu
Looking for a new/used copy of: THE STUDY OF PLANT STRUCTURE: PRICIPLES AND SELECTED METHODS. 1981. O'Brien, T.P. and McCully, M.E. Termarcarphi Pty. Ltd. Melbourne, Australia.
Also, looking for steel (non-disposible, and glass blades for a Spencer 820 American Optical Rotary Microtome.
Thank you in advance Please reply via email: crow-at-aloha.net
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear List, } } We recently purchased a microwave system for tissue fixation/embedding. } Unfortunately, the instruction book that we ordered on it's use did not } come and we now find out that it is no longer available (through Ted } Pella). Does anyone have any information on procotols or know of any good } reference books on microwave fixation and embedding? Any information that } will get us going would be greatly appreciated. } } William R.McManus } Supervisor } Electron Microscope Facility } Department of Biology } Logan UT 84322-5305 } billEMac-at-cc.usu.edu } } } Bill, Here is a reference which may be of some help. Ted Pella equipment was used throughout: J Vet Diagn Invest 9:61-67 (1997) "Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique."
The Microwave Tool Book--A practical guide for microscopists by Gary Login and Ann Dvorak ISBN 0-9642675-0-0
Microwave Cookbook for Microscopists--Art and Science of Visualization by L. P. Kok and M. E. Boon ISBN 90-71421-20-1
Rick Giberson at Ted Pella should be able to give you some starting point protocols.
Patty Jansma Tel:520-621-6671 plj-at-manduca.neurobio.arizona.edu Arizona Research Labs Division of Neurobiology University of Arizona
On Mon, 6 Jul 1998 billemac-at-cc.usu.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear List, } } We recently purchased a microwave system for tissue fixation/embedding. } Unfortunately, the instruction book that we ordered on it's use did not } come and we now find out that it is no longer available (through Ted } Pella). Does anyone have any information on procotols or know of any good } reference books on microwave fixation and embedding? Any information that } will get us going would be greatly appreciated. } } William R.McManus } Supervisor } Electron Microscope Facility } Department of Biology } Logan UT 84322-5305 } billEMac-at-cc.usu.edu } }
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Greetings, } } I am looking for PTFE tubing to fit a Hexland cryostage. Does anyone know } who carries parts for this unit? } } Thanks, } Carl
Hi Carl, Hexland are now part of Oxford Instruments, Research Instruments, Tubney Woods, Abingdon, Oxford, OX13 5QS. UK. +44 1865 393200. Or try your local agent. If you do not know a local contact e-mail Judith Brock on judith.brock-at-oxinst.co.uk.
Regards, Ron =========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
We are looking for flat molds for embedding in acrylic resins which could be hermitically closed. Can one out of you send me the name of the companies which sells them.
Thanks a lot
Marc
------------------------------ SCHMUTZ Marc IGBMC 1 rue Laurent FRIES BP 163 F 67404 Illkirch Cedex FRANCE
We are currently trying to establish capability in our lab to look at die cross-sections. In previous work we mounted them in epoxy or acrylic and coated the samples, but I'd like to be able to do this without mounting and coating. I've been told that there are simple polishing fixtures one can by to do this, but I can't seem to find where to buy them. Perhaps someone out there could suggest a source? Thanks,
Janet Rice MCC Senior Member Technical Staaff rice-at-mcc.com 512-338-3266
} We are currently trying to establish capability in our lab to look at di= e } cross-sections. In previous work we mounted them in epoxy or acrylic an= d } coated the samples, but I'd like to be able to do this without mounting and } coating. I've been told that there are simple polishing fixtures one ca= n } by to do this, but I can't seem to find where to buy them. Perhaps someone } out there could suggest a source? Thanks
--------- BUEHLER supplies the MICRO-PRECISE(TM) line of IC cross-sectioning equipment including the Tripoint Polisher for SEM/TEM sectioning. We als= o supply a semi-automated system that might be appropriate for your application as well. For more information, please contact me directly.
Scott D. Holt BUEHLER LTD. PO Box 1 41 Waukegan Rd. Lake Bluff, IL 60044 (847)295-6500 http://www.buehlerltd.com
The technique you are describing is very well suited to our Tripod Polish= er and is most likely the tool you have heard about. The Tripod Polisher is=
ideal for preparing a cross section of a specific device and can be used = to prepare both SEM and TEM cross sections. The SEM cross section is actual= ly the "easy" part of the process and is referred to as our "first side polish" when preparing a TEM cross section. The Tripod Polisher is easy = to use and we have developed a large database of technical reports on its us= e. The Tripod Polishing technique and the tool were actually developed at I= BM East Fishkill and have been used in cross sectioning for many years.
Another alternative is our BiPod Polisher which is a simpler version of t= he Tripod Polisher and is designed primarily for SEM cross sectioning. The BiPod polisher can be mounted on our polishing machines for semi-automati= c cross-sectioning. I'll be pleased to send you additional information on any of these products if they sound of interest to you. You can also get=
some of the information off of our web site at http://www.southbaytech.co= m.
If you plan to be at MSA next week in Atlanta, you can get a thorough demonstration of the technique at our booth (#433). =
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by Janet Rice } ------------------------------------------------------------------------=
The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
We are currently trying to establish capability in our lab to look at die=
cross-sections. In previous work we mounted them in epoxy or acrylic and=
coated the samples, but I'd like to be able to do this without mounting a= nd coating. I've been told that there are simple polishing fixtures one can=
by to do this, but I can't seem to find where to buy them. Perhaps someo= ne out there could suggest a source? Thanks,
} We are looking for flat molds for embedding in acrylic resins which could } be hermitically closed. Can one out of you send me the name of the } companies which sells them.
} Marc -
The Ted Pella company has one. It's teflon, and should be sealed for polymerization with Thermanox cover slips. I know it works, because it was designed by Doug Davis, a tech in my (pre-retirement) lab at U.C.Berkeley.
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
Is anyone currently doing the Kleinschmidt procedure for visualization of DNA with TEM? Dave Long at Montana State University needs to cooperate with a microscopy lab to have some DNA samples shadowed at low angle with the Kleinschmidt procedure. Either contact him by phone (406-994-5239) or me by phone (801-378-2451) or e-mail. Thank you. W. M. Hess.
Hi, I was asked to post this information from Cynthia Goldsmith for all M&M '98 attendees.
Taking MARTA (Metro Atlanta Rapid Transport Authority) from Hartsfield International airport to the downtown hotels.
} The MARTA rail can be found at the end of the baggage claim areas. Take } the MARTA Northbound train to the Peachtree Center station (N1), then } follow the signs to either the Marriott Marquis (through the Peachtree } Center Food Court and across a ramp) or the Westin Peachtree Plaza } (across Peachtree Street). } } You could also mention (as Sandy has in the information brochure) that } the rail lines run North-South and East-West, with the transfer point at } the Five Points Station. The GWCC (Georgia World Congress Center) is on the } } E-W Line, at the W1 station (Omni/Dome/GWCC).
see y'all soon, beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
We have a couple of older Kevex Delta (III, IV) EDS units based on DEC PDP-1173's running RT-11 ver. 4.05. The units will not rollover to from Dec 31, 1999 to Jan 1, 2000, instead giving a date error. The RT-11 ver. 4.05 operating system is not year 2000 compliant. Once the Kevex software is up and running it will accept the year 2000 dates and run fine, however if you reboot the system the Kevex software takes its date from RT-11 which has to be pre-2000 in order for it to boot. What this means is that if we want to have the correct date on our printouts we'll have to change the dates both in RT-11 and the Kevex software every time we use the system. Currently this is the only date related problem we've noticed with the systems. Of course, we could upgrade to a PC based system or the RT-11operating system and DEC PDP-1173's could be upgraded. Both are now owned and supported by a company called Mentec (http://www.mentec.com/) and the new version of RT-11 (ver. 4.7) is y2000 compliant. An upgrade to ver. 4.7 would cost in the neighborhood of $2k. Mentec has indicated to me that although the new version is y2000 compliant, there are other changes and without input from the developers of the overlying Kevex software they can't guarantee that the Kevex software would run correctly. The PDP-1173 could be upgraded (bournoulli drives are no longer available) with SCSI cards and compatable peripherals and in fact would have to be since Mentec no longer supplys software upgrades on bournoulli's. For now, our strategy will probably be to change dates and hope for an upgrade to PC based systems.
kszaruba-at-MMM.COM wrote:
} Here's a general question that should stir up some comment... } } My department has sent out a standard questionaire for us to estimate } any problems our lab computers, software, or equipment might have with } the year 2000 date problem. Perhaps some of you have already gone } through this. I'm not a computer wiz, and naively thought that } everything I use would be fine, since I don't do any date calculations } like folks in the banking, insurance, etc. businesses do. However, the } more I hear about this problem the more confused I get. Is it true that } some older CPU's or BIOS programs will not handle the "00" date? So } some of our older equipment might not work even though the software we } are aware of has little reliance on dates?? Any microscope or software } vendors want to comment?? } } Hope this isn't stirring up needless fears or urban legends, but I have } definitely heard some alarmist opinions and would like to consult a more } technically aware and level-headed source (i.e. yourselves!). } } Thanks, } Karen } } -- } Karen Zaruba, kszaruba-at-mmm.com } BioMaterials Technology Center } 3M Center Bldg. 270-1S-01 } St. Paul, MN 55144 } } *The opinions above are my own, not necessarily my employer's*
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
Our Hitachi 3200H SEM came with a Magneto-Optic drive for storing images. When we transfer these to our PC however, the images show up without any of the labels (Mag. KeV, etc.). Has any one experienced this and does any one know how to correct this ?
Thanks to all who replied to this question. I should have said that the specimens, in the first instance, would be barnacle larvae. These are quite tough compared to a lot of other specimens that we have handled over the years. The study is to do with the question of settlement onto substrates.
The ideal would be to look at specimens with ESEM and then to allow them to grow on - do you think this is possible or would ESEM/radiation dose curtail normal development after exposure to the beam?
In this age of digital imaging, is a film recorder still useful? The last presentation I went to was all PowerPoint 'slides' but shown directly from a computer to a video projector, no film to be seen.
I am trying to decide if we should get one, to transfer digital images back to film and/or to make slides for presentations etc. If you have some opinions or experience with these things, could you let me know.
Thanks.
Jonathan Krupp Microscopy and Imaging Lab University of California Santa Cruz, CA 95064 (408) 459-2477 FAX (408) 429-0146 jmkrupp-at-cats.ucsc.edu **Area code changing to 831 as of 7/11/98**
Jordi writes ... } } Hi ! } } Our Hitachi 3200H SEM came with a Magneto-Optic drive for storing } images. When we transfer these to our PC however, the } images show up without any of the labels (Mag. KeV, etc.). } Has any one experienced this ...
Yes ... on a different system ... but at least my JEOL system allows an "export" option which "burns" the info you want into the TIF (... assuming it is TIF files you are archiving ...). I respond because we actually prefer that JEOL doesn't do this ... as we prefer to annotate with better software (e.g., Photoshop). If we've lost track of our notes and the associated mag with each image ... we've realized we can load the image into Windows' Wordpad and search for the mag in the TIF header ... we can then pick an appropriate micron bar dimension from a table associated with micron/pixel for each mag ...
Also ... you might ask Photoshop to show you the TIF header info ... you might be able to find the info you're after if your software adhered to TIF's standard comment fields whereas JEOL didn't.
... hope this helps :o)
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
In response to Donald Lovett who wanted to know about using ferritin as a tracer for the crab. I did a study several years ago using ferritin mixed with india ink as a tracer to study circulation in the squid Loligo. The india ink helped us see when the solution was in the circulatory system. Mix 5 mls of 2X artificial sea water with 5 mls concentrated ferritin (Calbiochem) and 2 drops of india ink (sonicate the ink for 15 mins to break up the chunks). You need to spin down the ferritin for 30 mins -at- 30K to concentrate the ferritin. Then perfuse the animal-it might be hard in a crab-for 30 mins -at- room temperature. Then submerge into a high osmolarity fix (ie 2.5% glut in 0.1M cacodylate buffer with 0.8M sucrose- 1500 mosm). It worked really well. We also tried lanthanum and HRP. The combo of ink and ferritn was the best and easy to see in the TEM. Good luck! JoAnn Buchanan Molecular and Cellular Physiology Stanford University School of Medicine Stanford, CA 94022
Jon, Film recorders, as you are aware, are quite expensive ($10K for a good one). For the same amount of money you could buy the computer and projector to show them with. It's tempting, for sure. I am dithering with this same question myself. The only difficulty with going digital is the complete reliance on the technology: what happens if your computer crashes, the digital projector breaks, or both are incompatible. In getting ready for the MSA meeting (right now, in fact), I opted to do the textual slides in PP but to produce conventional slides for the non-textual material. Regular slides are always going to be superior (as is the photographic medium) and everyone has a conventional projector. Now, if you are pressed for time and have access to the technology, then digital is wonderful. If you have to make the purchase now, then go digital unless you really need the resolution.
} In this age of digital imaging, is a film recorder still useful? The last } presentation I went to was all PowerPoint 'slides' but shown directly from } a computer to a video projector, no film to be seen. } } I am trying to decide if we should get one, to transfer digital images back } to film and/or to make slides for presentations etc. If you have some } opinions or experience with these things, could you let me know.
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy Neckers Building, Room 146 - B Wing Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
Jon, we use and will continue to use a film recorder. In our case a Lasergraphics machine. Our lab is entirely digital and to be honest rapid computer based Powerpoint presentations are easily produced and often used. However, when it counts (ie: in public, to your peers) nothing is as good as an excellent high resolution 35mm slide. Whether it is to show illusive immunogold particles on cryosections "I am not sure whether you can see this at the back" or the minutae of confocal or decon image stacks there is NO substitute for a decent film recorder used to make images from high resolution digital images.
Simon C. Watkins Ph.D. MRC Path Associate Professor, Director CBI University of Pittsburgh
Allied High Tech specializes in Materiallographic, SEM and TEM sample preparation. We have the necessary tools, techniques, and supplies to ensure the cross-section you achieve is excellent. Allied also has a new polishing system with a micro-positioning head and polisher for doing precision cross sections, the Multi-Prep and TechPrep 8. If a micro-precision head is not what you want, we also have a easy to use hand tool. The procedures and techniques we have developed work very well. We also have a new Diamond Lapping film brochure containing a pictorial guide illustrating the results that can be achieved with Allied's Diamond Lapping Film.
If you are attending the Microscopy show in Atlanta our booth number is 548.
Gary Liechty is your representative in Texas, but any of the staff at Allied would be happy to assist you with any specific question you may have. Allied's goal is total customer satisfaction and the entire staff is committed to it.
For more information you may contact us at 800-675-1118 and visit our web site at: http://www.alliedhightech.com
We look forward to serving you.
Sincerely,
Edward Hirsch
At 09:06 AM 7/7/98 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
************************************************* Edward A. Hirsch Product Application Specialist Allied High Tech 2376 East Pacifica Place Rancho Dominguez, CA 90220 ph: (800)675-1118 x245 fx: (310)762-6808 http://www.alliedhightech.com *************************************************
Is there an independent technican and/or dose any one on this list know an independent technican they could recommend to dissamble a Philips 300 TEM, located in Montreal, Canada?
} In this age of digital imaging, is a film recorder still useful? The last } presentation I went to was all PowerPoint 'slides' but shown directly from } a computer to a video projector, no film to be seen.
Joe: A very good question. I have been on both sides of this issue. Below are deciding factors in terms of which format I use. 1) How solid is the technology at the point where the lecture is to be given? The concerns here: I have Mac, they always use PC's, the right cable is not there. I made my presentation on Office 98 under Mac OS8.1 and they have System 7.1 loaded with PowerPoint 3. The mismatches can be endless and much harder to resolve when compared to changing a bulb in a slide projector. 2) Is the PowerPoint presentation better than using the slides? Is animation a key feature of the presentation? Or sound? Are the flying bullets better than the sharpness of the slide? How important is screen lumens? Bright slides work better than dim PowerPoint. It is hard to beat 2000 plus lines of slide resolution when compared to 640 by 480 pixels. They have a video projector with a bulb 500 hours on the otherside of dead. 3) How important is it to fix the presentation on the fly? You sized your slides to work in a 30 foot long room and you find yourself in a 100 foot hall. You can not resize 35 mm slides two hours before the lecture. Of course you can extensively rearrange a lecture in an airplane seat; but you sure can't do that with some 35 mm slides. 4) Can your presentation work best with twin screens: two images at a time? This is usually a snap with 35 mm slides but it is still pretty rough with PowerPoint. 5) One must always keep in mind the audience. Recently I saw a really slick computer controlled presentation. Some in the audience were put off by the excellent presentation because it was too slick. An analysis might help here: it was a research lecture being used to strut one's wares for a tenure-track teaching position. The students in the audience thought it was great. Older faculty who did not use computer media thought it was needlessly glitzy. Media proficient faculty were more focused on how the presentation was assembled rather than the message. Predictable but it still caught me off guard.
Finally back to your question. I use both and probably will continue to do so for the next several years. For really important lectures on the road, I have 35 mm slides for backup. The bottom line is, however, which format will the audience respond to best.
Blystone in Texas
Robert V. Blystone, Ph.D. {RBLYSTON-at-Trinity.edu} Professor of Biology Trinity University San Antonio, Texas 78212 210.736-7243 210.736-7229 FAX
I am trying to respond to a message from Reggie Beijer of the Netherlands Forensic Sciences Laboratory. Unfortunately, I have no contact information. Can anyone provide me with an e-mail or FAX address? Please do not reply to the ListServer. Thank you for your assistance (TIA).
Andy
Andrew W. Blackwood, Ph.D. Structure Probe, Inc. P.O. Box 656 West Chester, PA 19381-0656 Ph: 1 610 436 5400 FAX: 1 610 436 5755 e-mail: ablackwood-at-2spi.com WWW: http://www.2spi.com
The Laboratoire d=92Analyse des Materiaux (LAM) of the Centre de Recherche Public-Centre Universitaire of Luxembourg has immediate opening, on a fixed term basis, for a maintenance engineer
The LAM facility includes 1 SEM, 1 TEM, 2 D-SIMS (CAMECA IMS-4f, CAMECA IMS-LAM), 1 S-SIMS (CAMECA/Ion Tof SIMS III) and one machine under development optimized for MCs+ clusters. The machines in operation are used to perform analysis for industries or by students preparing their PhDs.
The maintenance engineer will take care of all instruments existing at LAM (trouble shooting on electronics, maintenance of the physics).
Candidates should have a degree in electronics (baccalaureat plus two to three years), a first experience in maintenance of UHV equipment and be fluent in English. Interviews will take place at LAM, no funding will be provided for overseas trips.
Please send a resume and a list of references to:=20 CRP-CU 162a, avenue de la Fa=EFencerie L-1511 Luxembourg
-------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public Centre Universitaire (CRP-CU) Laboratoire d'Analyse des Materiaux (LAM) 162a, av. de la Faiencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpcu.lu
We just got a film recorder here and I definitely think that they are worth it: (1) good 35mm film recorders record 4,000 lines of resolution (resolution up to a maximum of 4096 horizontal by 2732 vertical lines, or 4096 x 2732) , which generally excedes the resolution of most 35mm films (looking under a LM I can't see the scan lines on 100ASA/ISA film - NO pixelation), (2) the HIGHEST resolution computer projection systems I have seen are 1024 x 860 - for text these are "o.k." but we work so hard to get excellent microscopic images and do we want to loose it all? (Besides a good film recorder costs the same or less than the best projection system, and you don't have to drag the thing with you to insure you have the best images you can) (3) many MANY places don't have good quality projector systems, but almost every where has a slide projector, (4) images take a LOT of disk storage space, so the image presentations don't fit on a diskette - you really need to burn a CD to take along to the meeting (can you guareentee they'll have a Zip drive? Maybe?), (5) Big image files are slow on all but the best computers systems, (6) which system to design the program for? Mac? PC? (7) want to use your notebook computer? Is it upto the task? do you want to play driver games the hour before your talk? (8) For B&W images the best quality I've seen are reproductions on true B&W media (as many have said this holds ture for printers as well), what I do is record the B&W images in negative to standard B&W film (i.e. T-max 100), develop in my darkroom (20-30mins) dry and mount - beautiful positive B&W slides! (Warning: look carefully, apparently negative film is much more senesitive than slide films so you have to get a good film recorder for this.
The one thing I haven't tried yet is taking true digital images back through film, back to a photographic enlargment, and comparing this to a dye-sub print.
O.k., so that's my two cents.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
Look for the "Microwave Cookbook of Pathology. The art of microscopic visualization". by Mathilde Boon and L.P. Kok. Coulomb Press Leyden, Leiden, The Netherlands. ISBN 90-71421-10-4 bound. I have the second revised edition from 1988, but probably there is a newer one available now.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } On Mon, 6 Jul 1998 billemac-at-cc.usu.edu wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } Dear List, } } } } We recently purchased a microwave system for tissue fixation/embedding. } } Unfortunately, the instruction book that we ordered on it's use did not } } come and we now find out that it is no longer available (through Ted } } Pella). Does anyone have any information on procotols or know of any good } } reference books on microwave fixation and embedding? Any information that } } will get us going would be greatly appreciated. } } } } William R.McManus } } Supervisor } } Electron Microscope Facility } } Department of Biology } } Logan UT 84322-5305 } } billEMac-at-cc.usu.edu } } } } } } }
Bill, }
Here is a reference which may be of some help. Ted Pella } equipment was used throughout: J Vet Diagn Invest 9:61-67 (1997) } "Four-hour processing of clinical/diagnostic specimens for electron } microscopy using microwave technique." } } Drs. Marcel Paques Wageningen Centre for Food Sciences p.a. NIZO Food Research Kernhemseweg 2, 6718 ZB Ede P.O.Box 20, 6710 BA Ede The Netherlands Phone: (31) 318-659690 Fax: (31) 318-650400 e-mail: paques-at-nizo.nl
Jon, "Yes" everyone is going to PowerPoint slides but" no" not everyone has a powerbook that they can take to meetings, courses, etc. Having made countless slides using PowerPoint and a digital slide maker, I don't know how we would manage without both at this point.
Example, I recently returned from a 3 week trip to China. Both my husband and I gave talks at 2 major Universityies on what was otherwise a vacation trip. There was no way we were going to drag a powerbook along. Even if we had, the University lecture halls were not equipped with digital projectors or LCD panels for projection of the PowerPoint slides directly from the computer program.
We have been in countless other situations where we wanted to make informal presentations in rooms which were not equipped with digital projectors such as when our extension educators go out to give talks to local groups. It is easy to come up with a 35mm slide projector, set it up and in no time be able to informally present data in the form of slides but more difficult to find suitable digital projectors, etc. for multiple people from one department to use at the same time in different parts of the state.
It is also very easy to tailor a presentation in just a few minutes by having an assortment of slides and picking and chosing for the audience at hand....much faster and easier that going through multiple PowerPoint files to find the slide desired and then copying and rearranging into a new presentation.
Debby Sherman +++++++
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-aux.btny.purdue.edu Purdue University or: emcenter-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057 --------------------------------------
Dear List:
In this age of digital imaging, is a film recorder still useful? The last presentation I went to was all PowerPoint 'slides' but shown directly from a computer to a video projector, no film to be seen.
I am trying to decide if we should get one, to transfer digital images back to film and/or to make slides for presentations etc. If you have some opinions or experience with these things, could you let me know.
Thanks.
Jonathan Krupp Microscopy and Imaging Lab University of California Santa Cruz, CA 95064 (408) 459-2477 FAX (408) 429-0146 jmkrupp-at-cats.ucsc.edu **Area code changing to 831 as of 7/11/98**
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Would you please recommend a short course for Light Element X-Ray Analysis
I have missed Lehigh's class that took place in June of '98.
Thank you in advance.
Darlene Peterson Harvey Eltech Research Center Fairport Harbor, Ohio
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We will be setting up interviews for candidates for the Fall teaching = position at San Joaquin Delta College for electron microscopy. Course = details are below. If you are interested in teaching all 4 courses or 2 = of them, please contact me in Atlanta at the Microscopy and Microanalysis = Meeting July 12-16, 1998. I will be staying at the Marriott Marquis = Hotel. You can leave a message there or on the meeting bulletin board. = A message left at the hotel might be faster. I will be coming into = Atlanta Saturday July 11 and leaving Thursday evening, July 16, 1998.
Please bring your active resume with you. I will have official SJDC = applications which you can fill out. You will of course need names, = addresses and dates of past employers and names, addresses, and phone = numbers for recommendations and typical application type information. We = can fax them to our Human Resources Dept, and then, if appropriate, set = up a conference interview with the search committee from Atlanta. Please = contact me as early as possible in the week. We are up against an = extremely tight deadline.
Thanks
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
URGENT: ADJUNCT FACULTY NEEDED FALL Semester 1998 (Aug. 12 - Dec 18, 1998)
A San Joaquin Delta College Faculty member of the Microscopy Technology = Center is planning a Sabbatical Leave for the Fall '98 semester. San = Joaquin Delta Community College is currently searching for an EM = Instructor Adjunct / Temporary One Semester Contract for Fall 98 (Aug. 12 = - Dec 18, 1998).
MINIMUM QUALIFICATIONS: Bachelor's Degree plus two years of directly related experience OR an = Associate Degree plus six years of directly related experience OR a valid = credential.
DESIRABLE QUALIFICATIONS: Master's or PhD Degree in a Biological = Science; Experience in teaching / practice of Electron Microscopy
COURSES TO BE TAUGHT:
Introductory Techniques for Transmission Electron Microscopy (EM21) This is a lecture/lab course which includes beginning Transmission = Electron Microscopy dealing with the alignment and operation of the TEM, = vacuum techniques, photographic techniques, as well as the preparation of = particles and replicas for viewing in the TEM. Includes individual = training in the use of the TEM, preparation techniques, and written and = oral reports. (Lec - 2 hrs; Lab - 3 hrs/wk)
Biological Ultrastructure (EM28) Course contents include specific information about the fine structure and = function of cells and tissue at the ultrastructure level. Videos, slides = and micrograph examination will be correlated with the lectures so that = students will learn to recognize the fine structure of cells and tissues = in relationship to their function. (Lec-2 hrs/wk)
Advanced Techniques in Biological Electron Microscopy (EM37) Course contents include lecture and laboratory which covers advanced = techniques for biological specimen preparation in TEM including an = advanced research project.( Lec - 1 hr; Lab - 6 hr/wks)
** Current Microscopies: Optics, Theory and Application (EM30) Course contents include information related to the physical laws and = applications of the various types of current microscopies e.g. = TEM,SEM,FIB, AFM, and confocal microscopy, as well as other current = topics e.g. asbestos analysis, lab design, etc. (Lec - 2 hrs; Lab - 3 = hrs/wk) ** This course is negotiable. A portion of this assignment would be = paid as an additional overload.
The above teaching load would not require development of new courses as = these courses have been fully developed. Course materials are available = to aid in the instruction. TERMS OF EMPLOYMENT: Full semester, Non-tenured track position with Full = Benefits (Medical, Dental, & Vision). SALARY for Fall 98 semester: BA/BS w/ 2 yrs experience or AA w/ 6 yrs experience$17,498 - 25,333 for Fall 98 sem
MA/MS$19, 219 - 28,137 for Fall 98 semester
PhD$ 22,528 - 31,739 for Fall 98 semester
Stockton, CA is 90 miles Northeast of San Francisco and 50 miles South of = Sacramento. It is in lush, green, San Joaquin Valley. We do not suffer = from the high rent districts of San Francisco and Silicon Valley, both of = which are our neighbors.
Additional SJDC Microscopy Technology Program Information available at = http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html/
If you do not contact me in Atlanta, you can contact Human Resources at = the below number however we are up against an extremely tight deadline = and a search committee that may only be available during the Atlanta = meeting. I will have official applications at the meeting and will fax = them to Human Resources.
APPLICATION: Contact Human Resources at 209/954-5056. Human Resources San Joaquin Delta College Admin. Bldg. Rm 202 5151 Pacific Ave Stockton, CA 95207
by gcsin1.gecm.com (8.9.0/8.9.0) with SMTP id SAA16375; Wed, 8 Jul 1998 18:04:29 +0100 (BST) Received: from gcschm.geccs.gecm.com by gcsin3.geccs.gecm.com; (5.65/1.1.8.2/13Mar95-1139AM) id AA18376; Wed, 8 Jul 1998 18:03:57 +0100 Disclose-Recipients: prohibited
Janet, you can either spend lots of dosh on a tripod polisher and polishing system, or do the following;
1) Get some glycothalate (?spelling) thermoplastic wax, such as 'crystalbond' (lots of electron microscopy suppliers sell it).
2) Get some epoxy - anything will do, 5 minute (Devcon) saves a lot of time
3) Glue up a 'sandwich' of three pieces of silicon using the epoxy, with the die you want in the middle. Do it one at a time, using as little glue as possible. Make sure everything is really clean, and squidge the samples about from side to side to squeejee the excess glue away. You can speed up curing on a hotplate. Make sure that all the pieces line up on one edge so that it stands on edge for when you section it.
4) Mount the sandwich, edge on, in the wax (melts at about 120C, nice and runny at 180C) in the centre of a standard polishing platen. Put three blocks of silicon round the edge.
5) Grind till flat on 1200 grit, polish for a few seconds on 2400 grit, fine polish on 1um diamond and a short nap felt pad (slow rotation) for 10 mins.
This is essentially tripod polishing without spending any money. If you want to hit a specific area, you can use a piece of glass instead of the top silicon piece, or even a small piece of silicon so you can see the uncovered part of the die and work out where you are from a (previously taken!) photo.
Let me know if you want any more details,
Richard Beanland GMMT Ltd., Caswell, Towcester, Northants NN12 8EQ UK
I think that it is absolutely worthwhile to get a film graphics recorder. While computers with video projectors are probably the wave of the future, at most presentations that I attend, there is the old Kodak Carousel and the 35 mm slides being projected. While a lot of commercial enterprises have the new computerized slide shows, I still believe that 35 mm slides are going to be around for a long time to come, especially in academic institutions. Not every institution nor department can afford the notebook and video projector just to be state of the art in presentation technology. Nor can many investigators for that matter.
I get called on a lot to either shoot 35 mm slides on my film graphics recorder for presentations and often at short notice.
The other thing that you should do as regards the need in your own facility is to ask your customers (the investigators at UCSC) if they use 35 mm slides in their presentations in order to gauge the need for a film graphics recorder. I will bet that the majority of them do. Their Powerpoint presentations as well as any digital microscopic images (confocal or otherwise) can easily be shot on 35 mm slide film with a film recorder.
Matthew J. Schibler Ph.D. Imaging Facilities Core Coordinator UCLA Brain Research Institute 73-384 CHS 951761 Los Angeles, CA 90095-1761
(310) 825-9783 FAX (310) 206-5855 E-mail: mschibler-at-bri.medsch.ucla.edu http:/btrip.mednet.ucla.edu/bri/homepage.htm } ---------- } From: jmkrupp-at-cats.ucsc.edu[SMTP:jmkrupp-at-cats.ucsc.edu] } Sent: Tuesday, July 07, 1998 3:40 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: LM/EM Film recorders? } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } Dear List: } } In this age of digital imaging, is a film recorder still useful? The } last } presentation I went to was all PowerPoint 'slides' but shown directly } from } a computer to a video projector, no film to be seen. } } I am trying to decide if we should get one, to transfer digital images } back } to film and/or to make slides for presentations etc. If you have some } opinions or experience with these things, could you let me know. } } Thanks. } } Jonathan Krupp } Microscopy and Imaging Lab } University of California } Santa Cruz, CA 95064 } (408) 459-2477 } FAX (408) 429-0146 } jmkrupp-at-cats.ucsc.edu } **Area code changing to } 831 as of 7/11/98** } }
One feature of film that has not been mentioned is its archival qualities. It is quite likely that digital presentations will not be very accessible in five years due to hardware and software changes. Properly processed film should last more than 50 years--probably more than 100 years. A slide can be viewed without any eletricity--hold it up to the sky! Try that with a Zip disk.
Has anyone used Image Space Software for looking at dual channel "single section" images. Specifically using the dual channel overlays with two colours. I may be missing something but I cannot get the dual or stereo applications to work with single images. Probably this is not possible with this software it seems to be looking for depth information.
Also which antifade agent do people recommend for fluorescent antibody probes for use with confocal.
I've been following the thread and I have two cents to add about something that I don't think has been touched upon.
I gave an internal presentation that had TEM images that were acquired from a Gatan 1024 x 1024 size CCD camera. The presentation was put together in powerpoint and printed out on a sub-dye printer on overhead transparencies. My manager asked me why I didn't do the presentation with the computer/projection system like several other people have recently done very elegantly. I told them that they didn't have images in their presentations and could get away with it. He said "what does that have to do with anything?"
So I told him the following things: 1) the best projection systems today have about 1024x780 pixel resolution. 2) my images are 1024 x1024 in size. If I wanted to show all the available details in the image, I would have to set the zoom on the powerpoint window so that only the image was visible and none of the title info or other stuff that I have on the slide would be visible. 3) on the sub-dye printer which has 300 dpi resolution, the same 1024 x1024 image would be 3.4 inches in size and I could put several images on the same slide which is then projected with all the details of all of the images in the slide.
Now I would prefer to use 35 mm slides when the absolute best quality in the images are required. However, in the last several years, I have converted to using overhead slides because of the ease of producing good quality overheads in a very short period of time and because slide projectors are becoming less and less available in some locations, particularly in the government labs. When I was at the Materials Lab at Wright Patterson Air Force Base, it was an ordeal to find a working slide projector when someone was coming through the lab that had slides. It is about the same here.
Ok, can I have the change left over from my two cents?
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
In this age of digital imaging, is a film recorder still useful? The last presentation I went to was all PowerPoint 'slides' but shown directly from a computer to a video projector, no film to be seen.
I am trying to decide if we should get one, to transfer digital images back to film and/or to make slides for presentations etc. If you have some opinions or experience with these things, could you let me know.
Thanks.
Jonathan Krupp Microscopy and Imaging Lab University of California Santa Cruz, CA 95064 (408) 459-2477 FAX (408) 429-0146 jmkrupp-at-cats.ucsc.edu **Area code changing to 831 as of 7/11/98**
It has become my task to convince our University here of the strategic importance in maintaining sophisticated microscopy, ie Confocal Microscopy, Electron Microscopy etc as a service to research and teaching.
Here in New Zealand the bulk of scientific research funding comes from the government via a small number of government funding agencies. The research climate is rapidly changing. It would seem the government is moving towards a situation where only a specific number of 'identifiable and targeted' research outcomes will be contested for research funding. If your research activity does not fall into one of these 'targeted' areas and you can not get funding from some other source then you are essentially out of the 'research game'.
With regard to major scientific equipment purchases, such as electron microscopes, NMR's etc, this means that new equipment initiatives (and presumably replacement of existing equipment) will become 'end user driven' rather than, as in the past, 'provider push'.
Traditionally Universities here have obtained research funding on the understanding that the infrastructure to do that research was already in place within the university and, if major equipment purchases were required, then funding was based on current and anticipated usage from a number of researchers or groups, with funds coming from a number of different funding sources (most of which have gone now) and including a contribution from the university itself based on a teaching component. This model is now almost gone.
In the new model it would seem that equipment will only be purchased if it is integral to the efforts of a particular targeted outcome. The 'funding for teaching' aspect is yet to be resolved however it would seem that of the income received by universities from the government to teach students, a 'research component' will also be contestable. This makes capital equipment, such as electron microscopes, NMR's etc, quite vulnerable unless the University can be convinced that it is in it's strategic interest to maintain the availability of such technology and techniques (funding of university teaching activity is also probably going to change soon with the possible introduction of a 'student voucher' system).
As I said above it has become my task to convince our University of the strategic importance in maintaining sophisticated microscopy, ie Confocal Microscopy, Electron Microscopy etc as a service to research and teaching. If such technology and techniques are considered of strategic importance then there is a better chance that the university will continue to maintain such facilities. Obviously I have a vested interest in the long term survival of our Microscopy Unit but I also believe that microscopy as a technique and technology continues to have vital role to play in many scientific disciplines and industrial processes therefore must continue to be taught to our science students.
1. Has anybody out there in 'microscopy land' had to convince their institution of the strategic importance in maintaining sophisticated microscopy within that institution ?
2. If so, I would appreciate some feed back on what arguments you thought worked and what arguments failed.
3. Did you succeed ?
All feedback would be appreciated.
Allan Mitchell
Richard Easingwood South Campus Electron Microscope Unit School of Medical Sciences University of Otago PO Box 913, Dunedin NEW ZEALAND
Microscopy/Microscopy Education offers a full range of customized, on-site courses. For more information, see our web-site at {http://www.MME-Microscopy.com/education} or call our offices.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education 125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site: {http://www.MME-Microscopy.com/education} ****************************************************** MME: America's first national consortium dedicated to customized on-site training in all areas of microscopy, sample preparation, and image analysis. Our goal: immediate growth in your productivity!
At 09:24 AM 7/8/98 -0500, Darlene Harvey wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
OK, I'll use propane instead of isopentane. Can someone tell me what purity of propane is required? I see 99%, 99.5% & 99.99 (!) % propane available. Airgas also has what they call "Natural grade (Grade 1.6)", 96%. (I assume there's a reason not to use propane containing mercaptans, oil, and who knows what else, from the cylinder of my propane torch or camping equipment. Right?) Can you recommend any US suppliers for 2 to 20 pound bottles? (2 pounds costs almost the same as 20 pounds. I don't need 100 pounds of propane.)
Thanks! Richard
} } } "Jim Darley" {jim-at-proscitech.com.au} 04/24/98 04:43am } } } Quite right, but now the discussion goes to: Which is the better cryoagent and that was a topic here a few months ago. Propane gas liquefied by cooling is a much, much better cryo-agent than is isopentane. Its easy to store in a lab a small gas cylinder with a blunt needle on a bit of tubing as the outlet. With little gas flow rub the needle over the small metal cup that is cooled by liq N2. Soon you will have a couple of ml of liquid propane. Do this in a fumehood, which is a good idea when using solvents too. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
I have a few extra rooms reserved at the Atlanta Marriott Marquis that I will not be using. I know the city is crowded next week so if anyone is looking for a room, please let me know ASAP as I plan to cancel these ext= ra rooms on Friday morning.
Use camping gas and I doubt you will find Any difference. None of the plunge cooling organic cryogens are as good as high pressure freezing (BUT that option although the best is expensive)
Patrick Echlion Multi-Imaging Centre University of Cambridge
On Wed, 8 Jul 1998, Richard Thrift wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } OK, I'll use propane instead of isopentane. Can someone tell me what } purity of propane is required? I see 99%, 99.5% & 99.99 (!) % propane } available. Airgas also has what they call "Natural grade (Grade 1.6)", } 96%. (I assume there's a reason not to use propane containing } mercaptans, oil, and who knows what else, from the cylinder of my } propane torch or camping equipment. Right?) Can you recommend any } US suppliers for 2 to 20 pound bottles? (2 pounds costs almost the same } as 20 pounds. I don't need 100 pounds of propane.) } } Thanks! } Richard } } } } } "Jim Darley" {jim-at-proscitech.com.au} 04/24/98 04:43am } } } } Quite right, but now the discussion goes to: Which is the better cryoagent } and that was a topic here a few months ago. } Propane gas liquefied by cooling is a much, much better cryo-agent than } is isopentane. Its easy to store in a lab a small gas cylinder with a blunt } needle on a bit of tubing as the outlet. With little gas flow rub the needle } over the small metal cup that is cooled by liq N2. Soon you will have a } couple of ml of liquid propane. Do this in a fumehood, which is a good } idea when using solvents too. } Jim Darley } } ProSciTech Microscopy PLUS } PO Box 111, Thuringowa QLD 4817 Australia } Phone +61 7 4774 0370 Fax: +61 7 4789 2313 } Great microscopy catalogue, 500 Links, MSDS, User Notes } **************************** www.proscitech.com.au ***** } }
We run courses "in house", in your own laboratory on your own equipment, on all aspects of electron microscopy. Please take a look at our web site for more information, we are able to tune a course to any requirement.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
For a quick review of the new technologies on exhibit at next week's Microscopy & Microanalysis meeting, see the July issue of American Lab: "Focus on Microscopy: What's New at M&M '98", pp. 41-45. (Hot off the presses!)
Extra copies will be available at the meeting at the Microscopy/Microscopy Education Booth (#510).
Richard, you should not have visited that propane variety store, its too confusing. I have used the propane supplied for camping and home stoves. Very likely this contains minor contaminants, but it worked well. Contaminants would change the freezing point in a minor way but I think it very unlikely that they would penetrate and affect cell structure. However, there is an "interesting" research project for some doubter. Please let me know after such "propane purity" research has been accepted for publication. Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes service-at-proscitech.com.au *** www.proscitech.com.au
-----Original Message-----
OK, I'll use propane instead of isopentane. Can someone tell me what purity of propane is required? I see 99%, 99.5% & 99.99 (!) % propane available. Airgas also has what they call "Natural grade (Grade 1.6)", 96%. (I assume there's a reason not to use propane containing mercaptans, oil, and who knows what else, from the cylinder of my propane torch or camping equipment. Right?) Can you recommend any US suppliers for 2 to 20 pound bottles? (2 pounds costs almost the same as 20 pounds. I don't need 100 pounds of propane.)
Thanks! Richard
} } } "Jim Darley" {jim-at-proscitech.com.au} 04/24/98 04:43am } } } Quite right, but now the discussion goes to: Which is the better cryoagent and that was a topic here a few months ago. Propane gas liquefied by cooling is a much, much better cryo-agent than is isopentane. Its easy to store in a lab a small gas cylinder with a blunt needle on a bit of tubing as the outlet. With little gas flow rub the needle over the small metal cup that is cooled by liq N2. Soon you will have a couple of ml of liquid propane. Do this in a fumehood, which is a good idea when using solvents too. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
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OK, I'll use propane instead of isopentane. Can someone tell me what purity of propane is required? I see 99%, 99.5% & 99.99 (!) % propane available. Airgas also has what they call "Natural grade (Grade 1.6)", 96%. (I assume there's a reason not to use propane containing mercaptans, oil, and who knows what else, from the cylinder of my propane torch or camping equipment. Right?) Can you recommend any US suppliers for 2 to 20 pound bottles? (2 pounds costs almost the same as 20 pounds. I don't need 100 pounds of propane.)
Thanks! Richard
} } } "Jim Darley" {jim-at-proscitech.com.au} 04/24/98 04:43am } } } Quite right, but now the discussion goes to: Which is the better cryoagent and that was a topic here a few months ago. Propane gas liquefied by cooling is a much, much better cryo-agent than is isopentane. Its easy to store in a lab a small gas cylinder with a blunt needle on a bit of tubing as the outlet. With little gas flow rub the needle over the small metal cup that is cooled by liq N2. Soon you will have a couple of ml of liquid propane. Do this in a fumehood, which is a good idea when using solvents too. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
We are using LR White for post-embedding staining with colloidal gold. It is absolutely imperative that we get the best possible fixation for synapses. Synapses have a large lipid component. Has anyone tried tannic acid in combination with LR White? If so, how? p-phenylenediamine is known to protect against lipid loss during alcohol dehydration. Has anyone used it with LR White? If so, how? Anyone have any other ideas? We have at our disposal UV light and Progressive Lowering of Temperature techniques, as well as standard oven polymerization. We would so much appreciate any help we can get. We absolutely have to have good fixation of synapses this summer in order to get our next grant (and keep our jobs!) Our antigens tend to be very difficult to locate with Au in material fixed with osmium and embedded in epoxides, which, of course, would give us our best fixation of synapses.
"Can someone tell me what purity of propane is required? I see 99%, 99.5% & 99.99 (!) % propane available. Airgas also has what they call "Natural grade (Grade 1.6)", 96%. (I assume there's a reason not to use propane containing mercaptans, oil, and who knows what else, from the cylinder of my propane torch or camping equipment. Right?) "
I have just constructed an apparatus for freeze spraying in propane, with the help of instructions from Scott Russell, and have been told barbecue gas propane is just fine....in fact the impurities help to reduce the freezing point (I think that's right!) Good job, because the pure stuff costs a bomb!
-- Amanda Wilson awilson-at-sghms.ac.uk Assistant Manager, Electron Microscope Unit, St George's Hospital Medical School, Tooting, London, UK. http://sghms.ac.uk/em tel:? 0181 725 5220 fax:? 0181 725 3326
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{HTML} {BODY BGCOLOR=3D"#FFFFFF"} Richard wrote:
{P} "Can someone tell me what purity of propane is required?=A0 I see 99%, 99.5% & 99.99 (!) % propane available.=A0 Airgas also has what they c= all "Natural grade (Grade 1.6)", {BR} 96%.=A0=A0 (I assume there's a reason not to use propane containing {BR} mercaptans, oil, and who knows what else, from the cylinder of my {BR} propane torch or camping equipment.=A0 Right?) " {BR} =A0
{P} I have just constructed an apparatus for freeze spraying in propane, with the help of instructions from Scott Russell, and have been told barb= ecue gas propane is just fine....in fact the impurities help to reduce the fre= ezing point (I think that's right!)=A0 Good job, because the pure stuff costs a bomb! {BR} =A0
} OK, I'll use propane instead of isopentane. Can someone tell me what } purity of propane is required?...
In my experience, commercial grade propane, such as is sold for use in your barbecue, propane torch and campstove, works perfectly well for plunge freezeing or propane jet freezing. The major differences that I found between chemically pure propane (99%) and commercial grade propane were of course due to the impurites in the latter. For example: (a) the viscosity of commercial grade propane is higher, so I had to enlarge the bore of the propane jets to obtain the same jet velocity (and thus freezing quality), and (b) the freezing point of commerical grade propane is depressed several degrees, and the higher viscosity may slow nucleation, so that I had more time to prepare my specimen before the propane froze. I have also saved a bundle of money and a lot of trouble (since it's so readly available) with the commercial grade stuff. FYI, I have been using 20 lb containers for jet freezing and campstove containers for plunge freezing.
Bill McManus asked: } We recently purchased a microwave system for tissue fixation/embedding. } Unfortunately, the instruction book that we ordered on it's use did not } come and we now find out that it is no longer available (through Ted } Pella). Does anyone have any information on procotols or know of any good } reference books on microwave fixation and embedding? Any information that } will get us going would be greatly appreciated.
I'm not certain which specific book Bill meant, but we sell the Kok & Boon _Microwave Cookbook for Microscopy_ and Gary Login's _Microwave Toolbook_. We also have several microwave processing and microwave fixation procedures at our web site (address in my .sig, below).
Best regards, Steven E. Slap, Vice-President
******************************** Energy Beam Sciences, Inc. The Laboratory Microwave Company http://www.ebsciences.com ********************************
Just a quick note to say that my experience with propane freezing has been very similar to that of John Gilkey's.
---------------------
" In my experience, commercial grade propane, such as is sold for use in your barbecue, propane torch and campstove, works perfectly well for plunge freezeing or propane jet freezing. ......................" J. Gilkey
"Breakthroughs in biology are preceded by breakthroughs in microscopy."
I cordially invite you to come see Molecular Imaging's technology breakthrough in microscopy, which may dramatically change and speed up the processes in drug discovery.
This "Environmental / In Vitro Atomic Force Microscopy" (AFM) can deliver nanometer resolution time lapse imaging and physical property characterization.
Please come to scientific talks at The Protein Society, 12th Symposium in San Diego, July 25-29, 1998, on this technology and its revolutionary applications: - Prof. Stuart Lindsay, ASU; Oscillating Probe AFM Study of Titin Unfolding, Mon. July 27, 1998, 10:15AM - Dr. Peter Hinterdorfer, University of Linz; Austria (Molecular Recognition Force Microscopy) Antibody-Antigen Recognition Detected By Ultrasensitive Force Microscopy Sunday July 26, 1998, Molecular Recognition, Poster Session, 3:15 - 5:30PM
Or come for a live demonstration by Molecular Imaging at exhibition booth # 613.
If you can not join us in San Diego, please visit us at http://molec.com/BIO-AFM/protein
Respectfully
George Sibbald
PS: If you can not get to Protein come to the Microscopy Society of America Show in Atlanta, July 13-16, 1998 ____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; "Innovating Probe Microscopy" 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
Dear All: We are seeking to purchase a TEM simulation software named Mc Tempas runed on Mcintosh computer. We appreciate it if any one can provid the information about where we can buy it, or suggest a better one that can perform interface structure simulation with supercell generation option. Thank you.
---------------------------------------- Dr. Dai Jiyan IMRE National Univ. of Singapore 10 Kent Ridge Crescent Singapore 119260 ----------------------------------------
I am interested in touring the CDC in Atlanta while attending the M&M98. Are there any official tours set up for this? It would be very interesting if we could have a tour geared towards microscopists. Is any one interested?
Sally Burns
Center for Electron Optics burnssal-at-pilot.msu.edu (517) 355-5004
Attention Poor and Desparate EM facilities: -I have three DENTON 515-DV available, used, operational, crated and ready for surplus to any Govt agency who wants it. Just let me know and get your GBL ready. If there are no Gov't takers, then Universities, then Schools are eligible. -I also have two LN2 tanks, on wheels, probably 25L size. Ready to go. -Misc LKB Ultramicrotome repair parts, like fuses, washers, ETC, freebies. -Eight LAB6 filaments for a JEOL 35, never used. You will need the ultra-vacuum pump for these. We never did buy one. -Small Sorvall Histo-knifemaker. That's it for now. I will ship small items today, or after the EMSA meeting. If you are there, I will bring them for you. BIG items will be shipped when the shipping paper work is completed. Please use E-Mail, so I will have a record of the time and date that you responded. Most equipment has service record and instructions. Thank you, Thomas A Baginski, USUHS, Bethesda, MD 20814 Email: tombg-at-bictom.usuf1.usuhs.mil or Voice phone for specific questions 301 295 5691
HILDEGARD CROWLEY wrote: } } Hi, } } We are using LR White for post-embedding staining with colloidal } gold. It is absolutely imperative that we get the best possible fixation for synapses. Synapses have a large lipid component. Has anyone tried tannic acid in combination with LR White? If so, how? } p-phenylenediamine is known to protect against lipid loss during } alcohol dehydration } snip {
Hildy:
I'm pretty sure p-phenyleaminediamine only protects against lipid loss when used after osmium. Sorry I don't have more to offer.
Geoff -- *************************************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane Piscataway, NJ 08854 voice: (732)-235-4583; fax -4029 e-mail: mcauliff-at-umdnj.edu ***************************************************************
I would like to look at the cross section of silicon nitride right next to the fracture surface. There is not enough room for dimpling . I was considering tripod polishing, creating a wedge on the side face perpendicular to the fracture surface and hopefully I will get a thin region that is close enough to the fracture. I would appreciate comments or suggestions from people who have tried this.
Hildy, You did not indicate whether it is possible to perfuse fix your material. If so, you might want to check the reference "Liposits, ZS etal (1986). A combined light and electron microscopic immunocytochemical method for the simultaneous localization of multiple tissue antigens. Histochemistry 85:95-106"
I worked with Zsolt on this publication which involved perfusion of rat brains with high and low pH paraformaldehyde, vibratoming the brains, immunocytochemical reaction using the PAP-DAB method, OsO4 fixation and finally embedding in epoxy. It gave very good localization and excellent ultrastructure since membranes were fixed with osmium after ICC. We worked out a simple method of flat embedding the desired areas of the sections between plastic coverslips and then serial sectioning them so we could trace the synapses.
I'm sure that Zsolt has worked out a method to use colloidal gold since that time....probably using nanogold coupled with silver intensification. You should be able to do a literature search and find more recent publications.
Although I do not presently work with neuronal tissue, I would be happy to dig back into my notes after returning from MSA. Do feel free to contact me if you want additional information.
Debby Sherman =================================================== Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-aux.btny.purdue.edu Purdue University or: emcenter-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057
--------------------------------------
Hi,
We are using LR White for post-embedding staining with colloidal gold. It is absolutely imperative that we get the best possible fixation for synapses. Synapses have a large lipid component. Has anyone tried tannic acid in combination with LR White? If so, how? p-phenylenediamine is known to protect against lipid loss during alcohol dehydration. Has anyone used it with LR White? If so, how? Anyone have any other ideas? We have at our disposal UV light and Progressive Lowering of Temperature techniques, as well as standard oven polymerization. We would so much appreciate any help we can get. We absolutely have to have good fixation of synapses this summer in order to get our next grant (and keep our jobs!) Our antigens tend to be very difficult to locate with Au in material fixed with osmium and embedded in epoxides, which, of course, would give us our best fixation of synapses.
So long, Hildy
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Fellow microscopists:
I am looking for suggestions/references on doing immuno on lens from human eyes. For some reason I am under the impression that the texture of the lens may require special fixation and penetration of Ab's may be difficult. Does it become brittle under certain conditions, i.e. would paraffin or cryo- sectioning be better - that, of course, may depend partly on the Ab's.
Also, does lens contain any endogenous autofluorescence?
Thank you for any assistance.
Judy Trogadis Eye Research Institute and University of Toronto Toronto Hospital, Western Div. 399 Bathurst St. Toronto, Canada M5T 2S8
Hi Folks: I want to make some resin casts for SEM of murine skin. two questions, 1: which is the least viscous resin that works well (I need to get at the capillary microstructure) 2: which is the best perfusion method for this type of perfusion (we normally perfuse intracardially) Of course I would welcome any other tips from the wise on this method Thanks a lot Simon
----------------------------------------------------------------------- Simon C. Watkins Ph.D. M.R.C.Path Associate Professor Director, Center for Biologic Imaging University of Pittsburgh Pittburgh PA 15261 tel:412-648-3051 fax:412-648-8330 URL: http://sbic6.sbic.pitt.edu
Sorry if I am sending this message a second time, but I suspect the original message was not ssent out.
Fellow microscopists:
I am looking for suggestions/references on doing immuno on lens from human eyes. For some reason I am under the impression that the texture of the lens may require special fixation and penetration of Ab's may be difficult. Does it become brittle under certain conditions, i.e. would paraffin or cryo- sectioning be better - that, of course, may depend partly on the Ab's.
Also, does lens contain any endogenous autofluorescence?
Thank you for any assistance.
Judy Trogadis Eye Research Institute and University of Toronto Toronto Hospital, Western Div. 399 Bathurst St. Toronto, Canada M5T 2S8
Listers-I thought most of you would appreciate the moral of this story. I know it happens quite frequently.
Paula :-)
} } } } This is a weird but true story (with a moral) } } } } } } } } A complaint was received by the Pontiac Division of General Motors: } } } } } } } } "This is the second time I have written you, and I don't blame you } } for } } } } not answering me, because I kind of sounded crazy, but it is a fact } } } that } } } } we have a tradition in our family of ice cream for dessert after } } dinner } } } } each night. But the kind of ice cream varies so, every night, after } } } } we've eaten, the whole family votes on which kind of ice cream we } } } should } } } } have and I drive down to the store to get it. } } } } } } } } It's also a fact that I recently purchased a new Pontiac and since } } then } } } } my trips to the store have created a problem. You see, every time I } } } } } buy } } } } vanilla ice cream, when I start back from the store my car won't } } start. } } } } If I get any other kind of ice cream, the car starts just fine. I } } want } } } } you to know I'm serious about this question, no matter how silly it } } } } sounds: } } } } } } } } 'What is there about a Pontiac that makes it not start when I get } } } } vanilla ice cream, and easy to start whenever I get any other } } kind?'" } } } } } } } } The Pontiac President was understandably skeptical about the letter, } } } } } but } } } } sent an engineer to check it out anyway. The latter was surprised } } to } } } be } } } } greeted by a successful, obviously well educated man in a fine } } } } neighborhood. He had arranged to meet the man just after dinner } } time, } } } } so the two hopped into the car and drove to the ice cream store. } } } } } } } } It was vanilla ice cream that night and, sure enough, after they } } came } } } } back to the car, it wouldn't start. } } } } } } } } The engineer returned for three more nights. The first night, the } } man } } } } got chocolate. The car started. The second night, he got } } strawberry. } } } } } } } } The car started. The third night he ordered vanilla. The car } } failed } } } to } } } } start. } } } } } } } } Now the engineer, being a logical man, refused to believe that this } } } } man's car was allergic to vanilla ice cream. He arranged to } } continue } } } } his visits for as long as it took to solve the problem. } } } } } } } } And toward this end he began to take notes: he jotted down all sorts } } } } } of } } } } data, time of day, type of gas used, time to drive back and forth, } } etc. } } } } } } } } In a short time, he had a clue: the man took less time to buy } } vanilla } } } } than any other flavor. Why? The answer was in the layout of the } } } store. } } } } Vanilla, being the most popular flavor, was in a separate case at } } the } } } } front of the store for quick pickup. All the other flavors were kept } } in } } } } the back of the store at a different counter where it took } } considerably } } } } longer to find the flavor and get checked out. } } } } } } } } Now the question for the engineer was why the car wouldn't start } } when } } } it } } } } took less time. Once time became the problem - - not the vanilla } } ice } } } } cream - the engineer quickly came up with the answer: vapor lock. } } } } } } } } It was happening every night, but the extra time taken to get the } } other } } } } flavors allowed the engine to cool down sufficiently to start. } } } } } } } } When the man got vanilla, the engine was still too hot for the vapor } } } } lock to dissipate. } } } } } } } } Moral of the story: even insane looking problems are sometimes real. } } } } } } } } A better moral: chocolate ice cream cures vapor lock! } } } } } } } } } } } } }
Paula Sicurello UC Berkeley Electron Microscope Lab psic-at-uclink4.berkeley.edu phone: 510-642-2085 fax: 510-643-6207
Does anyone know of a list server or organization similar to MSA for on-line exchange of information to people in the Molecular Biology field? Thanks in advance for any suggestions.
Patrice Abell-Aleff Electron Microscopy Core Facility Mayo Clinic 200 1st Street SW Rochester, Mn. 55905 phone: 507-284-3148 fax: 507-284-9349 e-mail: abellaleff.patrice-at-mayo.edu
There is an opening for a TEM technician at Seagate in the Minneapolis area. Interested individuals please reply directly to "Deb", at address shown at the bottom of the position description by fax or by US mail. Please do not reply by email. Thank you.
Augusto Morrone Seagate Technology Augusto_A_Morrone-at-notes.seagate.com (612)844-5838
Position Open at Seagate Technology: TEM Technician. A TEM technician position is open at Seagate Technology, RHO, in Bloomington, MN. Applicants should have at least a 2-year technical college degree, or equivalent experience, in TEM basic operation and sample preparation techniques in the physical sciences. The main duties involve TEM sample preparation using standard grinding, polishing, dimpling and ion milling techniques, operation and maintenance of sample preparation and darkroom equipment, and may include operation of the TEM, and handling TEM data in the form of negatives and digital images. The TEM facility is installing a Philips CM200 and is projecting the purchase of a FEG TEM within a year. Seagate offers an attractive benefits package and salary commensurate with experience. Please send your resume to:
Seagate Technology Staffing Department 7801 Computer Ave South Bloomington, MN 55435 Fax: (612) 844-7008 Attn: Deb
I am going to purchase a digital camera for general lab usage. I need a digital camera that has a capacity of producing high-quality outputs from both color and B/W photos (the resolution has to be 1024 x 800). Your kind suggestions and advice on the right make and good deal in the market will be appreciated.
Thank you very much for your help.
H. You ********************************************************************* Hong You, Ph.D Dept. of Cell Biology College of Medicine University of Cincinnati Cincinnati, OH 45267-0521 Voice: (513) 558 3709 Fax: (513) 558 4454 email: youhg-at-email.uc.edu *********************************************************************
Dear colleagues, I am trying to find a relatively fast method to count synapses on rat brain sections. I have done immunohistochemistry, but no matter how thin I cut, the synapses are so numerous that it is impossible to count. In old papers they used to do densitometry, but I don't think that is accurate way of counting. I have tried on fresh- frozen or perfused brains embedded in paraffin. I also tried to plastic embed and attempted to count under LM using oil immersion objective. Also tried to do immuno on LR White embedded thin plastic sections, which didn't work very well. Everything to avoid using the EM (too much time consuming, considering that I have six groups of different animals to compare). I would very much appreciate receiving your suggestions. Thank you, Lilith
G'day all from Hot and Muggy Atlanta (at least from a Chicagoan's perspective).
Just a single announcement that this is the Microscopy &Microanalysis 98 Meeting week. I'm in Atlanta now and hopefully all will run smoothly on the Listserver.
Live video and Video Conferences are being broadcast/held from the Meeting Site, in addition a daily Newsletter of events is being posted all to the MSA WWW site.
You may login and virtually join the conference at the MSA URL of:
Dear all, As you will have noticed, there have been two recent ads posted to the list from non members (one for a sex site, and one for an email Marketing program). Whilst the vendors who subscribe to this group almost always behave in a responsible manner by sticking to the charter and not sending overtly commercial mailings and Nestor deals with the occasional abuse, we have no control about non list members who post onto the list.
I am aware that some other discussion groups use software that blocks postings from non list members. I suggest that it may be necessary for Nestor to configure the software that is used for this list to prevent posting from non list members.
What do other people think.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
On 9 Jul 98 at 14:21, The slowly moving finger of Mandayam V. Parthasarathy wrote:
} -------------------------------------------------------------------- } ---- The Microscopy ListServer -- Sponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------- } ---. } } Just a quick note to say that my experience with propane freezing } has been very similar to that of John Gilkey's. } } --------------------- } } " In my experience, commercial grade propane, such as is sold for } use in } your barbecue, propane torch and campstove, works perfectly well for } plunge freezeing or propane jet freezing. ......................" J. } Gilkey } } ******************************************************************* } } M.V. Parthasarathy } Prof. of Plant Biology, Adjunct Prof. of Anatomy (Vet), &
On a completely different note. Has any work been done to characterise the Freon replacement gases ?? I can't find any ready reference to this and would like to find a safe replacement to Freon 12 and 22. Thanks John John Findlay Science Faculty EM Facility. Edinburgh University. Daniel Rutherford Bldg. Kings Buildings. Edinburgh EH9 3JH. tel. 0131-650-5344 fax. 0131-650-6563 John.Findlay-at-ed.ac.uk
Try Muller T, Moser S, Vogt M, Daugherty C & Parasarathy MV (1993). Optimisation and application of jet freezing. Scanning Microscopy 7, 1295-1310. Using HCFC 124 (SUVA 124-CHClFCF3) with thin titanium supports, cooling rates were obtained similar to those from propane and standard copper supports. This was suggestedt (I hesitate to plug this !!) in Ryan KP (1992) Cryofixation of tissues for electron microsocpy: a review of plunge cooling methods. Scanning Microsc. 6, 715-743. See next-to-last page , p. 742 in Discussion with Rerviewers section..
} I suggest that it may be necessary for } Nestor to configure the software that is used for this list to prevent } posting from non list members. } } What do other people think. Ian:
Well put, Ian. I agree. The sex post was the pits.
Blystone in Texas
Robert V. Blystone, Ph.D. {RBLYSTON-at-Trinity.edu} Professor of Biology Trinity University San Antonio, Texas 78212 210.736-7243 210.736-7229 FAX
Just use the delete key. Blocking postings from non-listers is a real drag. We have had many many many more legit postings from non-list members than from the ocational spammer. And the thing is, the spammers will co-evolve and get by whatever you try and throw at them. A lot of folks don't like to join because of the high volume of messages that are germane in general but not to them in particular. Just use your delete key. My 2 to the tenth electrons, Tobias } Dear all, } As you will have noticed, there have been two recent ads posted to the list } from non members (one for a sex site, and one for an email Marketing } program). Whilst the vendors who subscribe to this group almost always } behave in a responsible manner by sticking to the charter and not sending } overtly commercial mailings and Nestor deals with the occasional abuse, we } have no control about non list members who post onto the list. } } I am aware that some other discussion groups use software that blocks } postings from non list members. I suggest that it may be necessary for } Nestor to configure the software that is used for this list to prevent } posting from non list members. } } What do other people think. } } ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ } Ian MacLaren, Tel: (44) (0) 121 414 3447 } IRC in Materials for FAX: (44) (0) 121 414 3441 } High Performance Applications, email: I.MacLaren-at-bham.ac.uk } The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ } Birmingham B15 2TT, } England. } ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
I totally agree with you. I belong to several other list serv's none of which can be posted to unless you are a registered member. As an aside ... the sex site that was spammed to the list also got to several universities.......
} } As you will have noticed, there have been two recent ads posted to the list from non members (one for a sex site, and one for an email Marketing program). Whilst the vendors who subscribe to this group almost always behave in a responsible manner by sticking to the charter and not sending overtly commercial mailings and Nestor deals with the occasional abuse, we have no control about non list members who post onto the list.
I am aware that some other discussion groups use software that blocks postings from non list members. I suggest that it may be necessary for Nestor to configure the software that is used for this list to prevent posting from non list members.
What do other people think.
Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. { {
regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**I'm willing to make the mistakes if someone else is willing to learn from them**
I concur with Tobias. As obnoxious as they are, the best thing to do with junk mail is ignore it and throw it out. Remember the golden rule for junk mailers - never respond! Even telling them to leave you alone simply validates your mail address on their list which WILL be sold to someone else.
I think the value of this mail list far outweighs the junk mails I get because of it!
I hate when we get bogged-down in all of these "administrative" discussions, but since this could affect the list, here's my opinion:
Spamming is the e-mail version of junk-mail, and I haven't heard of any completely proven manner of removing junk mail without risking the reliability of the "real" mail. When you go through your mail at home, I'm assuming that you don't open everything mailed to you. You can guess that if it promises a million dollars, or has the mark of some credit-card company that you have never dealt with, you know its an ad, and can throw it away immediately, and not lose sleep over it.
My philosophy is: If the subject line doesn't have a topic related to my research, THEN I IMMEDIATELY DELETE IT, even if someone forgets to put a subject line. The subject line is your "filter" that only requires a couple of your own brain cells to activate. Let's face it: I don't know any e-mail software that doesn't show the subject line before opening messages, and if you're worried about spamming, I don't think I've seen any "spam" that didn't have a suspicious sender address (No professional that I know of would send and e-mail from "Lisa" with no last name!), or a suspicious or missing subject line.
This listserver is a forum for discussion, so I have responded to this topic, but let's please not get away from the SCIENTIFIC DISCUSSIONS!
Thank you for listening.
Gregg Sobocinski Parke-Davis Pharmaceuticals Ann Arbor, Michigan USA Note: This message is my opinion and has nothing to do with my employer. } -----Original Message----- } From: Ian MacLaren [SMTP:I.MacLaren-at-BHAM.AC.UK] } Sent: Monday, July 13, 1998 5:57 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Ads from non list members } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } Dear all, } As you will have noticed, there have been two recent ads posted to the } list } from non members (one for a sex site, and one for an email Marketing } program). Whilst the vendors who subscribe to this group almost } always } behave in a responsible manner by sticking to the charter and not } sending } overtly commercial mailings and Nestor deals with the occasional } abuse, we } have no control about non list members who post onto the list. } } I am aware that some other discussion groups use software that blocks } postings from non list members. I suggest that it may be necessary } for } Nestor to configure the software that is used for this list to prevent } posting from non list members. } } What do other people think. } } ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ } Ian MacLaren, Tel: (44) (0) 121 414 3447 } IRC in Materials for FAX: (44) (0) 121 414 3441 } High Performance Applications, email: I.MacLaren-at-bham.ac.uk } The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ } Birmingham B15 2TT, } England. } ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ }
Another method that does require some practice but is faster than the jigs and glass wheels is a very simple 2 step method that can be done when time is crucial. Obtain:
1) Pencil size XACTO knife holder. 2) 600 grit SiC paper. 3) MICROCLOTH - polishing cloth from Buehler Ltd. 4) 0.05 micron deagglomerated alumina or 0.02 micron colloidal silica.
The procedure is as follows:
1) Insert the die of interest into the end of the XACTO. 2) With a polish wheel rotating ( in the CCW direction with 600 grit paper, hold the die and holder assembly at 45 degree angle and begin to slowly grind (on the right hand side of the wheel) to the area you desire to cross section. You must frequently monitor the progress with an optical microscope. 3) Once you are 10 - 15 microns away (or so) from the area of interest, stop grinding. Rinse well and dry. 4) Mix up a slurry of 0.05 micron powder and apply to a MICROCLOTH. 5) Begin polishing the die on the left hand side of the wheel at a 90 degree angle to the plane of the wheel. Again, frequent inspection of the progress is needed. The colloidal silica can be used if a very fine artifact free finish is needed, but note that the silica will NOT polish tungsten plugs. When using the colloidal silica, try to find a polish pad that is made of spongy material.....i.e. neoprene or some such, the silica works with MICROCLOTH but a sponge-like material works better. The CMP supplier RODEL has some nice 8" CMP pads that fit the bill. 6) Rinse, clean and dry the specimen and then delineate layers with the appropriate acids
Hope this helps out. We use both methods in our lab but when time is crucial and the volume is large this technique saves the day.
John Staman Consulting FA Engineer Analytical Services Lab Symbios Inc. Colorado Springs, CO. 719-573-3282 ---------- -----------------------------------------------------------------------.
We are currently trying to establish capability in our lab to look at die cross-sections. In previous work we mounted them in epoxy or acrylic and coated the samples, but I'd like to be able to do this without mounting and coating. I've been told that there are simple polishing fixtures one can by to do this, but I can't seem to find where to buy them. Perhaps someone out there could suggest a source? Thanks,
Janet Rice MCC Senior Member Technical Staaff rice-at-mcc.com 512-338-3266
I agree with Tobias that at the moment, there are not sufficient violations of the rules to prevent legitimate postings from non-members. However, this could change. The net is still in its infancy. Ciao for now, Ken
I strongly disaggree with those of you who would simply roll-over and live with junk e-mail posted to a private e-mail list. Will you still be hitting the delete key when half the postings were never invited? ... or will you be unsubscribing?? I belong to several lists and monitor one myself and can attest to this list being especially vunerable (... for some reason ...). This list's monitor really should look into different list server software.
[to Nestor: I really do understand you have a real job ... and do sympathize with some of this not being under your control ... that is, I don't get to choose the list software this university uses ... but please pass these concerns on to your lists-meister ...]
... my $0.02 :o)
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I agree that these Spam postings are an annoyance. It would be nice if one could reply to them and overload their server. I know in fact that this is a bad idea, as it gobbles bandwidth, but it would just feel good to give them a dose of their own clutter. I know there is a web site to deal with this issue, I will find it and if there is any useful information I will post to the server. For now the delete key sounds best and fastest, if not the most emotionally satisfying.
-- Respectfully, Bob ( Robert G. ) Lawrence Failure Analyst Motorola Phoenix Corporate Research Lab 2100 E. Elliot Rd. MD EL-703 Tempe, AZ 85284-1806 Phone: 602-413-5848 Fax: 602-413-4952 Pager: 1-800-759-7243 PIN 834-2458
} [snip] } } ...I suggest that it may be necessary for } Nestor to configure the software that is used for this list to prevent } posting from non list members. } } What do other people think. }
It is easy to set most software to not allow non-members to post to a list. However, turning off automatic subscription and then vetting would-be subscribers is a tremendous amount of work. Since there is no vetting for subscription to this list, there is no protection from folk automatically subscribing to post an ad. I'll even bet that's how those ads got on here.
The degree of security one imposes on a list is directly related to the amount of work necessary to administer and maintain the list.
While I subscribe to a couple of lists where subscription is not automatic, they all cater to rather small groups. It's a bit much to ask Nestor to perform the vetting function. Until such time as Nestor gets extra pay to maintain the list and until such time as we decide to pay for his infrastructure, I suggest that Nestor can do whatever the hell he wants.
Dear all, As you will have noticed, there have been two recent ads posted to the list from non members (one for a sex site, and one for an email Marketing program). Whilst the vendors who subscribe to this group almost always behave in a responsible manner by sticking to the charter and not sending overtly commercial mailings and Nestor deals with the occasional abuse, we have no control about non list members who post onto the list.
I am aware that some other discussion groups use software that blocks postings from non list members. I suggest that it may be necessary for Nestor to configure the software that is used for this list to prevent posting from non list members.
What do other people think.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Nestor gave a report on this very problem yesterday to MSA Council, sponsors of this List. He is well aware of the problem but the solutions are difficult. Nestor's a little busy right now here at M&M'98 in Atlanta (check out the WWW site), but will probably reply himself when he has a chance.
Ernie Hall MSA Secretary
---------- From: Ian MacLaren[SMTP:I.MacLaren-at-BHAM.AC.UK] Sent: Monday, July 13, 1998 5:57 AM To: Microscopy-at-sparc5.microscopy.com Subject: Ads from non list members
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
Dear all, As you will have noticed, there have been two recent ads posted to the list from non members (one for a sex site, and one for an email Marketing program). Whilst the vendors who subscribe to this group almost always behave in a responsible manner by sticking to the charter and not sending overtly commercial mailings and Nestor deals with the occasional abuse, we have no control about non list members who post onto the list.
I am aware that some other discussion groups use software that blocks postings from non list members. I suggest that it may be necessary for Nestor to configure the software that is used for this list to prevent posting from non list members.
What do other people think.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
One camera that might be suitable for your requirements is the svMicro digital camera. This new camera will be available September 1st; it is a c-mount, resolution of 800x1000, 3 shot color camera, live black and white video preview & operates as a Photoshop plugin. On a MAC the svMicro is a SCSI device; on a PC, it is a parallel device. The CMOS chip is sensitive enough to image bright fluorescent subjects. The price is $2,200 as a c-mount and $2,600 with a package that includes lenses & extension tubes. For more information, please feel free to contact me at telephone 516-773-4305.
Matt Irwin ElectroImage, Inc. 277 Northern Blvd Suite 101 Great Neck, NY 11021
I am in the process of creating a web page for irusers-l, an internet listserv for scientists and conservators around the world who use infrared spectroscopy to study historic and artistic works and other cultural property.
The page will include links to commercial suppliers of FT-IR instrumentation and materials.
If lurking company reps would like to e-mail me company names, contact information, and URLs I'll try to include them in the page.
I'm realizing my PhD and I research about an halophyte, Atriplex nummularia Lindl. I work with plant anatomy and I'm looking for a stain that marks the vesicular hairs that are in this specie. Would you help me? Thanks.
i am working on the localization of antigens being involved in the adhesion/invasion process of pathogenic bacteria. Pre-embedding labelled samples are embedded in a resin which is suitable for perfoming post-embedding labelling afterwards like Lowicryls etc. I would like to know if someone has got any experience in using silver-enhancement kits for enlarging the gold-particles of the pre-embedding labelling with subsequent post-embedding labelling of the same ultrathin section.
- does the used resin has any effect on enhancing the gold-particles of the pre-embedding label
- does the silver-enhancement solution penetrates the entire ultrathin section and developes not only those gold-particles being exposed on the surface of the ultrathin section
- does silver-enhancement allows to perform postembedding labelling afterwards.
In the past, I've seen discussions on the list about the effects of vibrations from roadways, "floods" (both water and liquid nitrogen), ambient dust etc. on electron microscopes, but none have addressed lightning.
It may sound funny, but this is serious business.
It wasn't a direct hit, but it certainly "fried" a good deal of the electronics in our SEM. 2 1/2 weeks ago lightning struck the nearby power plant that supplies us with electricity, wreaking havoc all about in our industrial park. All the local production facilities managed to get back running within hours or a day of the disaster. Our SEM is *still* down, despite repeated visits of the SEM manufacturer's technical service people, who have been having trouble finding all the problems. This does not reflect well on the manufacturer...nor on us, for choosing this manufacturer.
But I'm interested in preventing similar problems in the future. Here in Germany, lightning is not all that frequent and at home I have *never* experienced a power outage due to thunderstorm action (20 years). But I think our power plant in the industrial park may be "lightning prone", since this is the second hit it's taken in the past 5-6 years. (The first time, we had no SEM.)
I'd be interested in suggestions about protecting our SEM from such hits to the power line in the future. Surely there are SEMs in more lightning-prone areas (Natal, South Africa? Southeast US?), where such protection must be routine. Can anybody give me some ideas?
Cynthia Bennett Hoechst Diafoil Germany
************** The opinions expressed here are solely my own and not the fault of my employer. **************
I think that very general "what do you people recommend" inquiries are best sent to the inquirer only and that person could then broadcast a summary. Another camera was touted here, so I wish to advise that Pixera has: 1260x940 pixel, comes complete with lens and other accessories, has new, superior software, offers most pixel/$ (even before the recent substantial price-reduction). The Pixera patent also gives better depths-of-field for it's pixel size than any other camera. A lot of technical info is provided in our online. Disclaimer: Yes, ProSciTech supplies Pixera and I would not have made this rather commercial posting had not another supplier preceded. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
From: H.You {youhg-at-email.uc.edu} To: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}
Lightning strikes and the resulting spikes are a problem, especially for us on the lightning-prone 'Highveld' of South Africa. Ordinary lightning protection diode and voltage dependent resistors are not necessarily fast enough to protect your EM. We use a UPS to protect EM's. You do not need a bigger capacity than one that will carry the microscope for a few minutes, so they are not too expensive. You do, however, need one that fully isolates the microscope from the mains supply. Take care to get one that outputs a clean sine waveform, even under full load - some of the UPS systems produce a pretty nasty square wave when working hard.
Jan Coetzee
} } Hi Listers, } } In the past, I've seen discussions on the list about the effects of } vibrations from roadways, "floods" (both water and liquid nitrogen), } ambient dust etc. on electron microscopes, but none have addressed } lightning. } } It may sound funny, but this is serious business. } } It wasn't a direct hit, but it certainly "fried" a good deal of the } electronics in our SEM. 2 1/2 weeks ago lightning struck the nearby } power plant that supplies us with electricity, wreaking havoc all } about in our industrial park. All the local production facilities } managed to get back running within hours or a day of the disaster. } Our SEM is *still* down, despite repeated visits of the SEM } manufacturer's technical service people, who have been having } trouble finding all the problems. This does not reflect well on the } manufacturer...nor on us, for choosing this manufacturer. } } But I'm interested in preventing similar problems in the future. } Here in Germany, lightning is not all that frequent and at home I } have *never* experienced a power outage due to thunderstorm action } (20 years). But I think our power plant in the industrial park may } be "lightning prone", since this is the second hit it's taken in the } past 5-6 years. (The first time, we had no SEM.) } } I'd be interested in suggestions about protecting our SEM from such } hits to the power line in the future. Surely there are SEMs in more } lightning-prone areas (Natal, South Africa? Southeast US?), where } such protection must be routine. Can anybody give me some ideas? } } Cynthia Bennett } Hoechst Diafoil } Germany }
Prof Jan Coetzee Head: Lab for Microscopy and Microanalysis Tel:+27-12-420-2075 University of Pretoria Fax:+27-12-362-5150 Pretoria 0002 Internet:janc-at-ccnet.up.ac.za South Africa http://www.up.ac.za/science/electron/emunit1.htm
Cynthia Bennett wrote ================================== In the past, I've seen discussions on the list about the effects of vibrations from roadways, "floods" (both water and liquid nitrogen), ambient dust etc. on electron microscopes, but none have addressed lightning.
It may sound funny, but this is serious business.
It wasn't a direct hit, but it certainly "fried" a good deal of the electronics in our SEM. 2 1/2 weeks ago lightning struck the nearby power plant that supplies us with electricity, wreaking havoc all about in our industrial park. All the local production facilities managed to get back running within hours or a day of the disaster. Our SEM is *still* down, despite repeated visits of the SEM manufacturer's technical service people, who have been having trouble finding all the problems. This does not reflect well on the manufacturer...nor on us, for choosing this manufacturer.
But I'm interested in preventing similar problems in the future. Here in Germany, lightning is not all that frequent and at home I have *never* experienced a power outage due to thunderstorm action (20 years). But I think our power plant in the industrial park may be "lightning prone", since this is the second hit it's taken in the past 5-6 years. (The first time, we had no SEM.)
I'd be interested in suggestions about protecting our SEM from such hits to the power line in the future. Surely there are SEMs in more lightning-prone areas (Natal, South Africa? Southeast US?), where such protection must be routine. Can anybody give me some ideas? ===============================================
I think the reason is in temporary increase of mains voltage. Methods of protection: - autonomous generator - most reliable method, - motor-generator with additional protection of the motor, - high-power BACK UPS (with constant converting of mains voltage) with additional protection of input circuits - most modern method. Regards.
Victor Sidorenko, ANTRON Co. Ltd., Moscow, Russia. antron-at-space.ru
For what it's worth, I agree with Gregg. I'm not brain dead yet (despite the long hours) and I can figure out which e-mail I want to read and which I want to delete. "For your eyes only", as a subject line, was a dead giveaway!!!
I'm sure that Nestor has enough to do without having to protect us all from a few pranksters.
} Hello all, } } Does anyone know of a list server or organization similar to MSA for } on-line exchange of information to people in the Molecular Biology field? } Thanks in advance for any suggestions. } } Patrice Abell-Aleff } Electron Microscopy Core Facility } Mayo Clinic } 200 1st Street SW } Rochester, Mn. 55905 } phone: 507-284-3148 } fax: 507-284-9349 } e-mail: abellaleff.patrice-at-mayo.edu
Nestor needn't go to the trouble to monitor subscriptions to the list or block mail from certain domains, but as the 'list owner' he should probably do his part to eliminate the spam at its source (see below), if he isn't already. Unsolicited bulk commerical email is a huge drain on the network bandwidth, disk space and other resources of network providers and SMTP relay sites. Furthermore, unlike bulk postal mail, for whch the sender pays at least part of the cost, the cost of bulk email is paid by us consumers in increased ISP charges, slow netowrks, etc. I started to receive several spams a day in my personal email about two years ago. Aside from the tangible and intangible costs, I consider spamming to be in intrusive and irresponsible use of network bandwidth, so I started sending messages to the postmaster and administrative contact (obtained from a WHOIS lookup, http://rs.internic.net/cgi-bin/whois) of the originating sites (watch for forged entries, though), their ISP, if any, and the postmaster of all relay sites listed in the message header (who can put pressure on the originating site to take action - again, watch for forged entries). By last spring, I had received notification of the termination of the accounts of several dozen of these spammers, and the rate of spamming had dropped to a trickle (1-2 per week). Some might think that by sending these messages, I was only adding to the noise, but while a single bulk mailing consists of thousands of messages, only a handful of people complain, and this is sufficient to alert postmasters to the problem and eliminate it at the source. I have in fact found that postmasters appreciate someone informing them of what is generally unwanted network traffic (for the relay sites) or a violation of the conditions of use (of a provider's services), so that they can deal with it effectively, since it is such a huge drain on their resources. Those spammers who have persisted and been brought to trial have generally been convicted on "theft of services" charges for this innapropriate use of other's equipment and abuse of priveleges. There is some remedial legislation in Congress, but unfortunately it does not go so far as to place the same sanctions on unsolicited commercial email as were placed on unsolicited commercial faxes ($500/message or cost to recipient, whichever is greater); see:
We are selling a JEOL 9000 freeze fracture unit. It needs a little work, but we never use it so we never had it repaired. We will accept the best offer we receive. You will have to pay for the shipping. If you're interested in this lovely putty & orange colored machine, please contact me. I promise that we will clean all of our junk off of it [we've been using it as a workbench ;)].
Looking forward to seeing the floor underneath it,
Paula :-)
Paula Sicurello UC Berkeley Electron Microscope Lab psic-at-uclink4.berkeley.edu phone: 510-642-2085 fax: 510-643-6207
In Tampa Bay, (FL, USA), touted as the lightening capital of the world, = lightening protection is a must. The local TV stations enjoy giving out = frivolous statistics such as the number of surface-to-cloud strikes per = hour. For example, night before last, there were more than 9000 strikes = in a one hour period. Hence, the potential of lightening damage is taken = seriously and has spawned an entire industry of consultants, manufacturers = and installers. The first line of defense seems to be the protection of = the building (every corner, projection etc. has a 12-18" lightening rod = and these are all connected), and there is massive protection on the main = power lines and communication lines. In fact, our computer network lines = are fiber even between floors of the main Hospital here. Most of this = falls under the responsibility of our main Hospital engineer and costs = many tens of thousands of dollars to install and maintain.
Although this is adequate for many applications, sensitive equipment = requires much more and that is even more expensive. I teamed up with the = rest of the Laboratory, and had installed a very large battery powered, = online UPS (takes up a whole room). From this we can run all essential = equipment for about ten minutes in the event of an outage, time enough for = the generators to kick-in. This device also smooths out generator noise, = and brown outs, (low voltage). In extremely simplistic terms, the = incoming current is disassembled on the incoming side, and reconstituted = on the outgoing side. The batteries not only act as a reserve and buffer, = but also a filter. All grounds are on this side of the UPS to prevent = ground loops, et cetera. At the time (about five years ago), this was the = most cost effective way to cover a multitude of bases for a large clinical = laboratory. =20
Intermediate-sized equipment, not in proximity to the large UPS, is on = individual battery powered online UPS. There is a wide range of sizes = available from vendors (for example, APC) for a wide range of dollars. = This is what I would expect is best for a single instrument such as a SEM. = Pay attention to wave shape and ratio for computerized equipment (vendors = can advise). If you cannot locate any local outlets, your network gurus = may be able to point you to their sources. Most network servers have such = equipment installed, albeit smaller than you probably require. The same = vendors usually sell the larger models as well (5 to 35 KW).
Our desktop computers are NOT on these devices. Instead, they are on = individual surge protectors only (for example, Tripplite surge protectors) = with no battery backups. One aspect about computers is that the majority = of strikes come across phone lines so a surge protector with phone line = protection is a must! That is, unless your institution has a switchboard, = which is already protected. At home, I experience at least one or two = such strikes a year that fries the modem protection on my computers. =20
A note of caution, do not place the inexpensive types of surge protectors = in serial, as it is possible to create destructive harmonics between the = devices.
Of course, like insurance, you must gauge the investment and risk with the = cost of protection.
Regards,
Peter O. Steele, Ph.D., PMIAC Dir., Special Anatomic Pathology Unit Pathology & Laboratory Medicine All Children's Hospital Saint Petersburg, Florida 33731-8920 v-mail: 813/892-4465 e-mail: steelep-at-allkids.org
We are thinking of putting uninteruptable power supplies (UPS) on our electron microscopes (Philips & Zeiss TEM's).
Any suggestions or comments?
Thanks - Dennis
------------------------------------------------------------------------------ Dennis C. Winkler, PhD. Phone: (301) 496-0131 Laboratory of Structural Biology Research Fax: (301) 480-7629 NIAMS, National Institutes of Health Email: winkler-at-calvin.niams.nih.gov Bldg. 6, Room B2-26, MSC-2717 Bethesda, MD 20892-2717, U.S.A.
I am trying to use a quantitative analysis program to analyze EDS spectra collected with TEM. I have 2 questions: (1) I have oxide inclusions in a diamond matrix . I think there are absorption problems (Carbon absorbing oxygen). Is there anyway of getting around it? I am using the "thin-section analysis" as an action. Should I use " bulk sample analysis"?
(2) I am using Nickel grid. So, the spectrum has Ni as an element in it. How do I calculate the formula of the mineral? Do I perform the quantitative analysis without the Ni? Or do I normalize the other elements without taking into account that the Ni is present? Is there anyway of determining if I do have Ni in my sample (or finding out proportions of Ni coming from the grid and from the sample?)
} We are thinking of putting uninteruptable power supplies (UPS) on our
} electron microscopes (Philips & Zeiss TEM's).
}
} Any suggestions or comments?
}
Dennis,
We have the Ferrups unit from Best Power on several instruments. There are two things that I would keep in mind:
1. make sure that you have the capability to handle the power surges that come from motors switching on and off (ie, pumps and compressors). For Best Power, this is the DVR option.
2. make sure that your physical plant people RTFM when installing the unit. For example, Best requires grounding cable that is *at least* the same gauge as the hot and common wiring. This requirement is more stringent than the typical electrical code.
Title: Optical Microscopy and Imaging in the Biomedical Sciences
When: October 7 - October 15, 1998
Where: Marine Biology Laboratory, Woods Hole, MA, USA
Tuition: $2050 (Includes room and board)
Application Deadline: August 4, 1998
Admission application and information: Carol Harnel, Admissions Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543-1015 (508) 289-7401 Internet: admissions-at-mbl.edu WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)
Course Director: Colin S. Izzard, State University of New York -at- Albany Phone: [518] 442 - 4367 EMail: csizzard-at-csc.albany.edu
Course Description:
For Whom: Designed primarily for research scientists, physicians, postdoctoral trainees and advanced graduate students in animal, plant, medical and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and video-imaging will benefit greatly from this course as well. There are no specific prerequisites, but an understanding of the basic principles of optics is desirable. Limited to 24 students.
The eight day course consists of lectures, laboratory demonstrations, exercises and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce photographic and video records for documentation and analysis.
Topics to be covered include: principles of microscope design and image formation bright field, dark field, phase contrast, differential interference contrast, interference reflection, and fluorescence microscopy confocal scanning microscopy and image deconvolution digital image restoration and 3-D reconstruction video imaging, recording, enhancement, and intensification analog and digital image processing and analysis fluorescent probes and ratio-imaging laser tweezers and laser scissors
Applications to live cells will be emphasized; other specimens will be covered as well.
Students will have direct hands-on experience with state-of-the-art microscopes, video cameras, recorders and image processing equipment provided by major optical and electronics companies. Instruction will be provided by experienced staff from universities and industry.
Students are encouraged to bring their own biological (primary cultures, cell lines, etc.) and material specimens and to discuss individual research problems with the faculty.
} We are thinking of putting uninteruptable power supplies (UPS) on our } electron microscopes (Philips & Zeiss TEM's). } } Any suggestions or comments?
Here's how it works in our lab:
The SEM and TEM are both running on a 220 V AC mains separated from the "ordinary". The separation is done by a 220 V AC generator driven by a 220 V AC electric motor. It seems to be a dull solution but this way we completely eliminate all electric surge and unwanted noise from the mains and besides the inertia of the two rotating iron cores is enough to overcome about 1/10 sec dropouts of the "ordinary" mains supply. We really do not need a UPS (taking into consideration the high power of TEMs and SEMs the UPS should be dimensioned quite big and expensive). During the past more than twenty years we had about two or three dropuouts long enough to shut down the microscopes. And the generator/motor system runs without problems, almost no maintenance, and cheap, even if you take into account the efficiency loss of the system. We even operate some sensitive computer servers and other instruments on this same local and very "clean" mains.
Kris
PS: The above solution also provides extremely effective protection against lightnings, see posting of Peter Steele ---------------------------------------------------------------------------- --------- Kristof Kovacs, Ph.D. Head, Central Laboratory University of Veszprem, P.O.Box 158, Veszprem H-8201 Hungary Phone: +36-(88)-421-684 Fax: +36-(88)-328-643
Hello, Do any of you know where I might obtain a copy of the book "Cell and Tissue Ultastructure- A Functional Perspective" by Patricia C. Cross and K. Lynne Mercer. W.H. Freeman and Co. 1993? It is out of print and I have checked the obvious resources. This is an excellent resource which I would very much like to add to my library.
I just joined the list. I love the infinite complex world. I really want a microscope and am interested in building one if it's cheaper. I've been researching home made telescopes too but cant find any information on making microscopes. Could someone help me? A powerful microscope would be great, like 1000x or larger, if possible (smaller's ok, I'd like at least 100x), but I'm assuming its similar to telescopes in that you can make pretty much any size given your time, money, and experience level. Perhaps someone knows of urls or books relevant?
thanks,
danny
ps- has anyone heard of Ernst Haeckel? He was a biologist who drew images of small complex life forms, here are a few examples of his art... I would love to be able to see these under a microscope
{A HREF="http://www.comptime.com/hoti/Haeckelview.html"} Haeckelview.html at www.comptime.com {/A}
In a message dated 7/15/98 12:28:50 AM US Eastern Standard Time, CALYK-at-aol.com writes:
} Microscopy-at-sparc5.microscopy.com
Hi there, when I was a younger person I used a book called wonders through the microscope to build a projection microscope. Gee, they even had plans in there to build your own arc lamp to use with it........a wonder we didn't burn the house down....
Anyway Check your local library there still may be a copy of this old tome there.
If all else fails maybe we even have an extra copy of it in the library here that we could trade off. The authors were the editors of popular science monthly. Edward Sharpe, Archivist SMECC
An NIH Director's Wednesday Afternoon Lecture Series Event Sponsor: NIH Inter-Institute Mitochondria Interest Group (MIG)
A Day-long Minisymposium=20 Mitochondria: Genetics, Health, and Disease 2 December 1998 Featuring The Wednesday Afternoon Lecture by Dr. Eric A. Schon (Columbia) Molecular Genetics of Human Mitochondrial Disease
Jack Masur Auditorium, Clinical Center, NIH For special accommodation needs call 301-594-5595 CME credit awarded=20 MITOCHONDRIA: GENETICS, HEALTH, AND DISEASE MINISYMPOSIUM 2 DECEMBER 1998
Lectures Venue: Masur Auditorium, Clinical Center, NIH Poster/Exhibitor Venue: Visitor Information Center, Clinical Center, NIH
* 0745 Registration, Poster Set-up, Continental Breakfast in Exhibit = Area * 0830 Dr. David A. Clayton (HHMI): Mitochondrial DNA Control Features * 0905 Dr. William C. Copeland (NIEHS): Avoidance of Mitochondrial DNA = Mutations by DNA Polymerase Gamma * 0940 Dr. Vilhelm A. Bohr (NIA): Oxidative DNA Damage Repair in = Mammalian Mitochondria * 1015 Poster Session/Coffee Break in Product Exhibit Area * 1045 Dr. Tracey Rouault (NICHD): Abnormalities of Mitochondrial Iron = Metabolism and Human Disease * 1120 Dr. Steven J. Zullo (NIMH): In situ Localization of the common = Human 4977bp Mitochondrial DNA Deletion Mutation * 1200 Lunch Break * 1330 Dr. Mariana Gerschenson (NCI): Mitochondrial Genotoxic and = Functional Consequences of Chemotherapeutic Drugs * 1400 Poster Session/Coffee Break in Product Exhibit Area * 1500 Wednesday Afternoon Lecture: Dr. Eric A. Schon = (Columbia):Molecular Genetics of Human Mitochondrial Disease * 1600 Reception/Poster Session in Product Exhibit Area * 1700 Poster Session/Product Exhibition Closes
Continental Breakfast, Coffee Breaks, Lunch Break, and Reception = sponsored by the Technical Sales Association Attendance/Poster Registration Form Mitochondria: Genetics, Health, and Disease Wednesday Afternoon Lecture Series Minisymposium 2 December 1998 Masur Auditorium and Visitor Information Center Clinical Center (Building 10), NIH Bethesda, MD
Note: There is no registration fee to attend this minisymposium! Name: Title: Affiliation: Address: State: Country: Postal Code: Telephone: Fax: E-mail: Web Page URL: I will attend and present a poster I will attend but not = present a poster________ Poster Title: Abstract (Abstract Booklet available at the meeting):
Do you need special accommodations while at NIH?______________ For a map of NIH and area, please check the following Web Site: = http://www.nih.gov/welcome/maps.html WARNING: Parking is limited on = campus, plan to use METRO! A block of rooms at a special meeting rate has been reserved at The = Bethesda Ramada. Call 800-272-6232, or 301-654-2703, before 9 November = 1998. Mention NIH Minisymposium, group # 6210. For other = accommodations in the area, please check the following Web Site: = http://www.patsys.com/ftd/city.cgi?pcityid=3D2521&pcity=3DBethesda Submit advanced registration via Minisymposium web site at = http://www-lecb.ncifcrf.gov/~zullo/migDB/symposium.html or via e-mail to: zullo-at-helix.nih.gov deadline by e-mail is 8 November = 1998=20 or via regular mail to: Steven J. Zullo, PhD postmark deadline is 2 = November 1998 Building 10, Room 2D54 NIH Bethesda, MD 20892
Steven J. Zullo, PhD Laboratory of Biochemical Genetics NIMH-NIH; Bldg. 10, Rm. 2D56; 9000 Rockville Pike Bethesda, MD 20892 301-435-3576; FAX 301-480-9862 zullo-at-helix.nih.gov Mitochondria Interest Group Web Page: = http://www-lecb.ncifcrf.gov/~zullo/migDB/
I was wondering if anyone has experience with the new Leica CPC unit. We are using this for freezing thin films to get virtified ice on holey carbon grids. We recentlly purchased one of these units and I would be extremely grateful for any tips and advice from someone with experience of using the unit for this application. How do you avoid ice contamination?
Many thanks in advance,
Dr Timothy F. Booth Canadian Food Inspection Agency National Centre For Foreign Animal Disease Suite T2300 1015 Arlington St. Winnipeg Manitoba R3E 3M4 CANADA http://www.hc-sc.gc.ca/main/lcdc/web/bmb/fedlab_e.html#toc email tbooth-at-em.agr.ca Tel 204 789 2022 Fax 204 789 2038
There are a number of sources of plans for home made microscopes; however, I really don't think you will save any money over the very low cost microscopes currently imported from China.
Here is the design I consider the most realistic for construction using only hand tools: Curry, Alan; Grayson, Robin F.; and Hosey, Geoffrey R. "Under the Microscope"; Van Nostrand Reinhold, New York, 1982; pp 31-40. (This design is/was featured on the Boston Museum of Science Web page)
I have a few ideas on how to simplify this even further, contact me if you are interested.
What is the current, generally accepted way of disposing of osmium tetroxide?
I use 1% osmium tetroxide in sodium cacodylate buffer. After removing this solution from my tissue, I pour it into a hazardous waste container that is 2/3 filled with corn oil to bind the osmium solution. We've done this for years, but our new hazardous waste/disposal monitor needs to have an official source with exact quantities of oil to osmium tetroxide ratios.
Can you help me? If there is safer, more acceptable method of disposal, your suggestions would be greatly appreciated.
Thank you in advance for you help and guidance.
Lucy Stribling Air Force Research Lab Brooks AFB Texas
I am considering the purchase of an evaporative carbon coater for use on coal clinker specimens that have a history of bad charging under the SEM. The evaporative carbon coaters (carbon rod source) come in two types: high vacuum (10^-5 torr) and low vacuum (10^-2 torr). The high vacuum coaters (with a turbo pump) are significantly more expensive. Are they worth the extra cost?
I'm told that www.abebooks.com is an excellent web site for out-of-print and hard-to-find books in the sciences and other areas. Haven't yet tried it personally, but a friend has had good success.
Good luck.
Randy
Randy Tindall Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003
We maintain a JEOL 2010 FEG-TEM for which a UPS is regarded by the manufacturer as essential. We use a Phase One Series 700 12.5 KVA unit that was suggested to us by JEOL. It works great and has plenty of capacity (right now it is running the scope and some accessories and only outputing 50% of its full capacity). It and the microscope have been in use for 5 months and with all the power outages due to thunderstorms here in Houston I don't know how we could live without it. If I could I would get one for our other microscope and every other piece of major equipment in our facility. So my thoughts these days are: UPS, Gotta' have it!
Disclaimer: I have no financial interest in any of the abovementioned companies
TTFN
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Roy Christoffersen Materials Science Research and Engineering Center University of Houston 3201 Cullen Houston, TX 77204-5932 roy-at-bayou.uh.edu (713) 743-8273 FAX: (713) 743-2787
I'm told that www.abebooks.com is an excellent web site for out-of-print and hard-to-find books in the sciences and other areas. Haven't yet tried it personally, but a friend has had good success.
Good luck.
Randy
Randy Tindall Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003
I'm told that www.abebooks.com is an excellent web site for out-of-print and hard-to-find books in the sciences and other areas. Haven't yet tried it personally, but a friend has had good success.
Good luck.
Randy
Randy Tindall Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003
Dear Barna, The science of EDS analysis on a TEM is a less well-characterized than on an SEM. I think that the results are semi-quantitative at best and it is difficult to know the exact conditions of film thickness and other sample characteristics for good analysis. Good quantitative analysis will require some known standards with a composition close to your unknowns. However, in answer to your questions: (1) Carbon will not appreciably absorb oxygen in your oxides. X-rays are absorbed by elements heavier than the emitting element. The absorbtion correction for oxygen in a carbon matrix, particularly in a thin film, will be very small. Use the "thin-film" analysis mode. (2) When you calculate the formula of your mineral, just leave the element of your grid out of the element list. The only way I know to tell if you have Ni in your sample is to use another grid. Copper, nylon, berylium, molybdenum, etc. are all available. Or, if your diamond matrix is strong enough, use a large-mesh grid, slot or single-hole grid so you can do measurements a long way from the grid material and see if your Ni response drops down to a minimal signal. You wrote: } } } Hi everyone, } } I am trying to use a quantitative analysis program to analyze EDS spectra } collected with TEM. } I have 2 questions: } (1) I have oxide inclusions in a diamond matrix . I think there are } absorption problems (Carbon absorbing oxygen). Is there anyway of getting } around it? I am using the "thin-section analysis" as an action. Should I } use " bulk sample analysis"? } } (2) I am using Nickel grid. So, the spectrum has Ni as an element in it. } How do I calculate the formula of the mineral? Do I perform the } quantitative analysis without the Ni? Or do I normalize the other elements } without taking into account that the Ni is present? } Is there anyway of determining if I do have Ni in my sample (or finding } out proportions of Ni coming from the grid and from the sample?) } } Thanks very much for your time and help, } } Please reply to: barna-at-geo.princeton.edu } } } } Barna } Best of luck, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca
... i am not quite sure, but I think that there has been a discussion about different dye-sub printers on this list-server... sorry for coming back to the same point, but here is my problem: we are planning to buy a high-end "photorealistic" printer. For certain reasons we tend to a Codonics ... Could anybody explain to me, what the advantage of the additionally implemented "direct thermal" technology (-} Codonics NP1660) is? The main task for our printer would B&W prints. The maximum percentage of colour prints is estimated to be in the region of 10 to 20%; pages/year appr.: 750..1000 ). It would be also very interesting to to have a comparison about the costs per page (Codonics NP1600 versus NP1660) including paper and ribbons, but excluding the investing costs for the printer itself.
Thank You very much in advance! Dipl.Ing.(FH) Gunnar Glasser, Elektronenmikroskopie
The coater type of choice for your application is the LOW vacuum type. For
some applications, low vacuum coater films are not as "good" as high vacuum types. In your case, however, the lower vacuum means more scattering. With increased carbon scatter, the coating is less "line-of-sight" and will better coat rough, convoluted surfaces.
I would also strongly suggest you investigate low vacuum coaters which use a
carbon yarn filament rather than the carbon rod type.
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I am considering the purchase of an evaporative carbon coater for use on coal clinker specimens that have a history of bad charging under the SEM. The evaporative carbon coaters (carbon rod source) come in two types: high vacuum (10^-5 torr) and low vacuum (10^-2 torr). The high vacuum coaters (with a turbo pump) are significantly more expensive. Are they worth the extra cost?
by imo24.mx.aol.com (IMOv14_b1.1) id NOCPa22587; Wed, 15 Jul 1998 13:45:13 -0400 (EDT) Message-ID: {7b79c6b5.35aceaab-at-aol.com}
If someone knows a contact at popular science mag. perhaps we can get permission to have a file of the projection microscope that plans that are downloadable on the MSA site?! We have the orig. book here that we could scan but hate to get our hands slapped by a publisher! I will leave this idea in the hands of some able diplomat that would like to contact popular science or who ever owns them now!
Sorry for the several postings about the books---"operator error" is to blame. A newly-learned fact: when a message is returned because of an improper primary address, the "cc:" address still goes out just fine. oops....
Randy
Randy Tindall Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003
Recently there was a message sent out about the Woods Hole microscopy course for the fall. In error I deleted it and would like to have a copy of it. Anyone have it to forward to me?
Hi, can anyone there help me to put the scale bar onto a confocal image? I saved confocal image with scale bar (with overlay option), but once I open the image with any other imaging program, such as PhotoShop, the scale bar is not on the image any more. I wonder what I did wrong and how can I save the confocal file with scale bar as well as make the scale bar occurs when other imaging softwares other than LSM operating software are used. Your help will be greatly appreciated. Thank you.
I have just acquired two ADEM1 SEMs and am now in a quandary as to what to do with them. It is not economical to get them running and sell them as the upkeep costs for a contract is about $30k per year. I could donate them to a school but I have already donated two Hitachi SEMs in the last two years.
They are worth more in parts than whole but I do not want to dismantle this "work of art". (besides neither the specimen or gun chamber makes an attractive flowerpot).
Both have windowless EDS detectors and a backscatter detector. One has an optical microscope and a WDS system.
Dear Fellow MSA Listserver Members: I noticed the intense debate via email of the recent postings of "junk email" or "spam" on this List. Recently a report from a study sponsored by the Federal Trade Commission (FTC) concludes that there is basically no easy solution to this problem, although the FTC is working to solve it. The Consumer Protection Bureau of the FTC has asked that junk emails be forwarded to them at: uce-at-ftc.gov In the last year, they have prosecuted 5 businesses and warned 1000 more about spamming. This is probably the most effective way to handle the spam problem without one or only a few persons of this list saturating their time to fight against it. See also: http://www.ftc.gov/opa/9807/dozen.htm and http://www.ftc.gov/opa/9807/jbspam.713.htm for more info.
Hi there, are you in Arizona? Heck I would give them a home, besides if you have 2 of something that just means you have spare parts to keep things going with! Of course having electronics background does help.... I have an amr1000 I have yet to get opp. but part of that is my own lack of follow through. Since I have one of them It would be nice to have 2 of them! Therefore, that fact you have 2 is a great asset to the person that wishes to do own maint.
Coaters with only a mechanical pump do not give a satisfactory coating for studying morphology at reasonably high magnifications in SEM and TEM. They are however, quiet satisfactory for EDS or WDS analyses. It's not a matter of money, but most applications simply require a diffusion or turbo pump for carbon coating. Also, sputtering of carbon is unsatisfactory (VERY SLOW) and a carbon sputter head is no solution to carbon coating at all. Rotating the specimen during evaporation is effective for dealing with "difficult to coat" specimens. Disclaimer: ProSciTech distributes EMITECH equipment in SE Australasia only. Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes **************************** www.proscitech.com.au *****
-----Original Message-----
To all you printer users
Why do people prefer the Codonics printer when the Kodak seems to be 3/4 of the price and uses the same engine? I see more comments on the list about the Codonics than the Kodak. Is it just to have a networked printer or is the software better? Our need is primarily TEM (monochrome) but with maybe 10% colour, any comments from users with experience of either (or both) of these would be welcomed.
Thanks Ron =========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
I think I know the answer to this one, but does anybody out there have a = need / interest in the manuals described above? I am about to discard = them, but I'm finding hard to let go....
Going, going... gone?
James Wesley-Smith Electron Microscope Unit University of Natal, Durban South Africa
Which scope? If you have a Zeiss 410, write back and I'll pass along the secret :)
Tamara Howard CSHL
On Thu, 16 Jul 1998, Ping Li wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi, can anyone there help me to put the scale bar onto a confocal image? } I saved } confocal image with scale bar (with overlay option), but once I open the } image } with any other imaging program, such as PhotoShop, the scale bar is not } on the } image any more. I wonder what I did wrong and how can I save the } confocal file } with scale bar as well as make the scale bar occurs when other imaging } softwares } other than LSM operating software are used. Your help will be greatly } appreciated. Thank you. } } Ping } pli-at-is.dal.ca } } } }
Two ways of doing this without burning the overlay into the image. 1. Perhaps the Zeiss software tells you the size of the field or the size of each pixel. Let's say each pixel is 0.285um and you want a 10um scale bar. Just divide to get the length in pixels to pop in with Photoshop. 2. Take an image of a micrometer slide.
-------------------------------------------- Michael Cammer email sent from an account of the Analytical Imaging Facility The Albert Einstein College of Medicine of Yeshiva University 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 FAX: (718) 430-8996 http://www.ca.aecom.yu.edu/aif/ --------------------------------------------
I have been trying to contact RMC at two different # and get a busy signal = for both. Can anyone give me their phone #, or e-mail address or any = explanation of what is going on? Thanks. Donna Wagahoff SIU School of Medicine Springfield,Il. 62794-1220 217-782-0898
Dr. Milos Kalab, a food scientist retired three years ago, has established a web site. Please visit following site if your are interested
http://www.cyberus.ca/~scimat/
He is still working hard in his laboratory.
Ann Fook Yang EM Unit Eastern Cereal and Oilseed Research Centre Agriculture and Agri-Food Canada 960 Carling Ave Central Experimental Farm Ottawa, Ontario Canada K1A 0C6
RMC 3450 So. Broadmont, Suite 100 Tucson, AZ 85713 Tel. (520) 903-9366 Fax (520) 903-0132 http://www.rmc-scientific.com/microtomes
Good luck, hope this helps!
Bob ***************************************** Robert (Bob) Chiovetti rchiovetti-at-aol.com E. Licht Company / 1-800-865-4248 Colorado/Utah/Arizona/Wyoming/ New Mexico/West Texas Representing Leica Since 1967 *****************************************
Two year ago we closed our EM Lab and I guess it is time I unsubscribe from this new group. (If some one would tell me how to.) Before I go I would like to share an essay I wrote just before we sold the EM and closed the lab.
To Whom It May Concern:
It will concern few, almost none, I suppose. Things change, seasons change. What were once considered major advances in the history of the human species, are regarded as important only to the past. I sit in a room which is cool, and dimly lit. There is a constant hum, so natural, that like breathing, it goes unnoticed. Today I hear this sound and try to embed it firmly in my memory. It is the sound of a voice I will not hear again. I focus on the sound, and struggle to imagine the sound of silence. All I can hear is the rhythm of the pump and the buzz of electronic. Most people see it as a huge piece of scientific equipment, just a Transmission Electron Microscope. It is not; it is a place. No No No. It is a magic carpet. It has taken me into the essence of life itself. I have crossed a cell membrane with more ease than sodium ions. I have followed an axon for microns, and watched as its microtubules dance in and out of view. I have observed actin and myosin locked in each other's embrace. I have beheld mitochondria in the thousands and found no two exactly the same. I have watched microvilli wave in rhythm like fields of wheat. I have looked at life (or rather the shadow of life) magnified 50,000 even 100,000 times. People have told me that this technology is obsolete, or they say that it is no longer cost effective for a small research department to support this type facility. Maybe it is their fault. Maybe it is the manufacturers fault, because they made service contracts too high. Maybe it is the government's fault, because it made money so tight. Maybe it is us for we have failed to see the direction science was turning and missed our chance to create the necessary techniques that would make this technology part of the wave of the future. Maybe it is my fault, because I did not show everyone all the things I have seen and places I have been. Astronauts have said that once you walk on the moon you no longer look at it the same way. NASA no longer goes to the moon and although it is still explores space, it seems to only be passing time. Like NASA, science will continue its exploration. Yes, just like NASA. I've not walked on the moon, but I have strolled where few people have tread. For those who have made my journeys possible I send my gratitude. It was definitely an "E" ride.
Thanks to those whom it did concern. To Mike, To Susan, To Ann.
Larry Hawkey, 1996 On the closing of the department's EM Lab.
Larry Hawkey hawkey-at-neuro.duke.edu Department of Neurobiology Duke University Box 3209 DUMC Durham, NC 27710 (919) 681-6425 fax (919)684-4431 http://www.duke.edu/~lah1
It is indeed a sad day when the accountants take over.
Managements general economic philosophy of restructuring, reorganising, reengineering, and retrenching unfortunately has no place in science and technology. Accountants (+snr man.) are reluctant to provide funding to reequip, repair, revitalise or replace.
LEO has a Cambridge S90 SEM for disposal (replaced by a new LEO 435). The SEM is at US Synthetics in Orem Utah. It was in working order when recently decommissioned, and has a BSD. Condition as seen, buyer collects, Any offers to Bill Neill billneill-at-csi.com please.
FOUNDRY RESEARCH INSTITUTE Research Materials Department Manager Structural and Physical Research Laboratory str. Zakopianska 73 Call (*48 12) 2665022 ext.356 30-418 KRAKOW - POLAND Fax (+48 12) 2660870
---------- Forwarded message ----------
Dear All,
We would like to purchase a rotatable (360 degree) X-Y stage for our Zeiss Axiovert 35M. Stages that fit the Axiovert 10 also fit this microscope. Even better yet, we would like to purchase just the X-Y part of this stage (Zeiss part number 45 35 60). If anyone has a used stage (or a new one), and would be interested in selling it, please contact me at Schwartz-at-rsbs.anu.edu.au
Thank you very much.
Sincerely,
Owen M. Schwartz
Owen M. Schwartz PhD Research School of Biological Sciences The Australian National University GPO Box 475 Canberra, ACT 2601 Australia
We are in the market for a new knifemaker. Those of you with newer model = knifemakers, please let me know how you rate their performance. Does anyone other that Leica make knifemakers? Thanks.
Donna Wagahoff SIU School of Medicine Springfield, Il. 62794-1220 217-782-0898 fax 217-524-3227
I've got a couple of users who would like to pursue Pre-embedment labling of plant tissue (trials with older knives and glass show excellent results) but are very concerned about potential damage to diamond knife edges. So I'm collecting opinions from experienced pre-embedment microtomists: are biological diamond knife edges basically 'safe' from 10-20 nm gold? Such that future biological sectioning will not suffer from any damage to the knife edge?
Thanks.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
Try amazon.com on the web. They just found an out-of-print ultrastruct= ure book for me. It took them about 6 months, but they finally came up wit= h a slightly used copy. =
We are putting together a proposal to purchase a inverted microscope system for imaging of live cells. Here are a few general questions that I would love some feed back on:
1. Is Multi-Photon Imaging only available thru BioRad? We don't have the expertise to build one and can't afford the BioRad system.
2. If given the choice between a filter wheel or a monochromator for the excitation wavelengths, what is the better choice? I am concerned about the amount of light you can get through a monochromator. I figure I can always reduce the amoant of light from the filter wheel.
3. Is mercury vapor the best choice for the light source?
4. If you have an environmental chamber, is it also neccessary to have an objective heater or stage heater in order to have versatility in the kind of samples you can image?
5. Have you found that in some circumstances an upright system with a water immersion objective is more useful?
Opinions concerning any of these questions would be greatly appreciated. Thank you.
Bob Underwood Derm Imaging Center U of Washington Box 356524 Seattle WA. 98195-6524
Suggestions for adjustable height chairs (or modifications thereof) that minimize or eliminate fiber shedding and static charge would be appreciated. Thank you.
I am requesting this information for a colleague who is interested in automatic processors for paper and film. Has anyone used the Mohrpro 8 processor and what is their experience with it? You may reply to my email address directly if you wish.
Regarding the Codonics or Kodak printers....They are both built on the Kodak engine and look virtually identical. They also use the Kodak supplies for Dye-Sublimation printing and should give similar output. The difference is indeed in the software. The Codonics has completely rewritten the software and added many unique features which makes the printer much more versatile. I have found that the option to send multiple files to the printer and have them scaled and printed on one page a very desirable feature. That way I can send 4 SEM files at a time and have them printed as 4x5 with the cost of only 1 sheet of paper. That gives quick high quality copies similar to what we get with polaroid but cost is much less with digital capture and dye-sub printing (~$.50 per 4x5). The cost of the 1600 is not very different from the Kodak printer.
The Codonic NP-1660 has the added feature of being able to print on large pieces of sheet film and is very useful for medical imaging. It also will be able to produce high quality thermal prints at a projected cost of about $.50 per 8x10. There have been production problems with the thermal paper but my understanding (having just returned from the MSA meetings where I talked to Codonics representatives) is that they hope to have the media available within 2-3 months. Use of this would drop the cost of 4x5 study prints to a very low $.13/print and is what sold me on this printer. This is a UNIX printer that can be easily networked and image files can be sent postscript, via telnet or via the WWW. There are a huge range of compatable file types.
I have had some techinical problems with our Codonics printer but have had very good service....either another printer has been shipped within 24hrs in the case of hardware problems, or they diagnose and suggest fixes for software problems over the phone. I have been willing to cut them a bit of extra slack over the delay in delivery of the thermal paper because they have been very responsive in many other ways.
Since Kodak may have added features I am not aware of....do carefully check the spec's on both so you can choose the best for your purpose.
Debby Sherman ========================== Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-aux.btny.purdue.edu Purdue University or: emcenter-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057
--------------------------------------
Hi there,
... i am not quite sure, but I think that there has been a discussion about different dye-sub printers on this list-server... sorry for coming back to the same point, but here is my problem: we are planning to buy a high-end "photorealistic" printer. For certain reasons we tend to a Codonics ... Could anybody explain to me, what the advantage of the additionally implemented "direct thermal" technology (-} Codonics NP1660) is? The main task for our printer would B&W prints. The maximum percentage of colour prints is estimated to be in the region of 10 to 20%; pages/year appr.: 750..1000 ). It would be also very interesting to to have a comparison about the costs per page (Codonics NP1600 versus NP1660) including paper and ribbons, but excluding the investing costs for the printer itself.
Thank You very much in advance! Dipl.Ing.(FH) Gunnar Glasser, Elektronenmikroskopie
I don't know about shedding, but I have used a spray can of "Static Guard" or some such product. I think it was made by 3M. It worked very well during our winters when the inside humidity hit bottom. Of course our summers are so humid that we don't have static problems now.
At 11:34 AM 7/17/98 -0400, you wrote: } Suggestions for adjustable height chairs (or modifications thereof) that } minimize or eliminate fiber shedding and static charge would be } appreciated. Thank you. } } James Martin
I am neither a Kodak or Codonics user, but understand the following.=20
The Kodak undoubtedly has some processor built into it to handle the printing functions, and it is apparently adequate for many functions.=20
The Codonics incorporates a Sun Sparcstation as the smarts for their printer. That is apparently what gives it more flexibility, processing (not printing) speed, and network connectivity. Of course it comes at a cost. I do not know what the differences between the two Codonics models are.=20
--------- At 08:28 AM 7/16/98 +0100, you wrote: } Why do people prefer the Codonics printer when the Kodak seems to } be 3/4 of the price and uses the same engine? I see more comments on the=20 } list about the Codonics than the Kodak. Is it just to have a } networked printer or is the software better? Our need is primarily TEM } (monochrome) but with maybe 10% colour, any comments from users with } experience of either (or both) of these would be welcomed. } } Thanks } Ron
---------- and someone else wrote:
Hi there,
... i am not quite sure, but I think that there has been a discussion=20 about different dye-sub printers on this list-server... sorry for=20 coming back to the same point, but here is my problem: we are planning to buy a high-end "photorealistic" printer. For=20 certain reasons we tend to a Codonics ...=20 Could anybody explain to me, what the advantage of the additionally=20 implemented "direct thermal" technology (-} Codonics NP1660) is? The main task for our printer would B&W prints. The maximum=20 percentage of colour prints is estimated to be in the region of 10 to=20 20%; pages/year appr.: 750..1000 ). It would be also very interesting to to have a comparison about the=20 costs per page (Codonics NP1600 versus NP1660) including paper=20 and ribbons, but excluding the investing costs for the printer itself.=20
Thank You very much in advance! Dipl.Ing.(FH) Gunnar Glasser, Elektronenmikroskopie
MME offers customized on-site courses in all areas of microscopy. We have several consultants who work in this area. If we can be of help either visit our website at { {http://MME-Microscopy.com/education} or give me a call here in the office.
FEI Company currently has openings for experienced service engineers on SEM, TEM or FIB systems. Current areas of opportunity include Oregon, Idaho, California, Georgia, Virginia, and Texas. However, we are always looking for experienced engineers in any area.
If you would like to apply or refer an applicant, please contact me directly.
~~~~~~~~~~~~~~~~~~~~~~~~ Sarah A. Boydstun-Brown Sr. Human Resources Generalist FEI Company Phone (503)640-7556 Fax (503)726-2754 www.feic.com ~~~~~~~~~~~~~~~~~~~~~~~~
Dear Barna, } } I am trying to use a quantitative analysis program to analyze EDS spectra } collected with TEM. } I have 2 questions:
Several sessions of the recent MSA-MAS meeting were devoted to just these sorts of questions, and the proceedings are available on-line. In addition to any replies you receive, I suggest you look up those papers which appear relevant to you and contact the authors. This is just the reason why I make every effort to attend the meetings as often as I can; all the experts are within reach.
} (1) I have oxide inclusions in a diamond matrix . I think there are } absorption problems (Carbon absorbing oxygen). Is there anyway of getting } around it? I am using the "thin-section analysis" as an action. Should I } use " bulk sample analysis"? } The only ways "around it" are either to use appropriate standards (which contain a carbon matrix and a known amount of oxygen) or to use a correction to the oxygen intensity which accurately accounts for the absorp- tion. As was repeated several times at the meetings, this correction is usually the one with the greatest uncertainty. If your experimental con- ditions are such that the beam penetrates the specimen with little loss in energy and with little spread, then thin-section analysis is correct, and bulk sample analysis, which includes calculations for the production of x-rays by electrons which have deviated greatly from the incident dir- ection and lost a large fraction of their energy (among other effects), will give the wrong answer. The right procedure to use will always depend on the experimental conditions, and one must understand how the analysis soft- ware relates to those conditions in order to select the appropriate form of analysis.
} (2) I am using Nickel grid. So, the spectrum has Ni as an element in it. } How do I calculate the formula of the mineral? Do I perform the } quantitative analysis without the Ni? Or do I normalize the other elements } without taking into account that the Ni is present? } Is there anyway of determining if I do have Ni in my sample (or finding } out proportions of Ni coming from the grid and from the sample?) } If you were sure that your specimen did not contain nickel, you could subtract the nickel peak along with the continuum background and calculate the formula from what's left. You could put your sample on a different kind of grid and reanalyse it in order to determine whether it contains nickel--if a qualitative analysis shows no nickel, then all the signal is from the grid, and you can safely subtract it. You can also look at an area of the original grid which has the diamond matrix with no inclusions to see if the nickel peak is significantly reduced. In this case, be sure to check the continuum-to-nickel ratio. What you are trying to determine is whether the reduction in nickel signal is due to your moving to an area which contains less (zero) nickel, or whether the reduction is due to fewer electrons being scattered onto the grid bars (or, for that matter, pole pieces or any other nickel-containing part of your instrument) and producing a signal. This can become quite complicated if the average Z of the inclusions is very different from that of the matrix, and can vary depending on how large the inclusions are with respect to beam diameter and specimen thickness (i.e., what fraction of the analysis volume is inclusion). Good luck. Yours, Bill Tivol
I appreciate what Nestor has done and is doing with respect to the listserver and it is a lot of work. Basically, I think that he is aware of the problem and if he wants to tackle the problem, it should be up to him. Until he decides to do something, I am already deleting a lot of stuff that I'm not interested in without reading it. A few extra in a week doesn't bother me. Yes, some of it is offensive, but usually I know from the subject that it is spam and I delete it without reading. Sometimes it gets through. I try not to let it ruin my day as I delete it and forget it.
Essentially, let Nestor decide when enough is enough.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Dear all, As you will have noticed, there have been two recent ads posted to the list from non members (one for a sex site, and one for an email Marketing program). Whilst the vendors who subscribe to this group almost always behave in a responsible manner by sticking to the charter and not sending overtly commercial mailings and Nestor deals with the occasional abuse, we have no control about non list members who post onto the list.
I am aware that some other discussion groups use software that blocks postings from non list members. I suggest that it may be necessary for Nestor to configure the software that is used for this list to prevent posting from non list members.
What do other people think.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren, Tel: (44) (0) 121 414 3447 IRC in Materials for FAX: (44) (0) 121 414 3441 High Performance Applications, email: I.MacLaren-at-bham.ac.uk The University of Birmingham, http://web.bham.ac.uk/I.MacLaren/ Birmingham B15 2TT, England. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} (1) Carbon will not appreciably absorb oxygen in your oxides. X-rays are } absorbed by elements heavier than the emitting element. The absorbtion } correction for oxygen in a carbon matrix, particularly in a thin film, will } be very small. Use the "thin-film" analysis mode.
X-rays are strongly absorbed when their energy is just above the energy for the transition from a filled state to a vacant state in the absorbing element. This topic is covered in Friedlander, et al. "Nuclear and Radiochemistry", and to quote, "...consider the K-alpha X rays of zinc (Z=30) which have an energy of 8.6 kV. The K absorption edges of Cu and Ni are 9.0 and 8.3 keV respectively. Therefore, nickel is a good absorber for zinc K-alpha X rays, and copper is not..." So your statement that x-rays are absorbed by heavier elements is not correct. The light-ele- ment folks would have memorized the O K-alpha and C K-edge energies (I haven't) and would know whether carbon absorption of O K-alpha is small or not. Yours, Bill Tivol
It depends on your microscope. If you use a coater system that does not have a clean vacuum system such as a turbo pump has, then you can get oil contamination on your sample that will show up in a high beam current density instrument such as a Field emission SEM. However, your coal samples could be a source of contamination even though you went with a more expensive system. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
I am considering the purchase of an evaporative carbon coater for use on coal clinker specimens that have a history of bad charging under the SEM. The evaporative carbon coaters (carbon rod source) come in two types: high vacuum (10^-5 torr) and low vacuum (10^-2 torr). The high vacuum coaters (with a turbo pump) are significantly more expensive. Are they worth the extra cost?
Dear Catherine, } } Can anyone please advice on the proper disposal of uranyl acetate? } Thank you. } In New York small amounts of 1% UAc can be poured down the drain. Of course disposal of kg quantities of UAc is a different matter. Also, disposal regulations may vary from state to state, so it is best to check with your safety office. If they don't know, check with your state's environment department (ours is Environmental Conservation, but the name could be different in your state). Yours, Bill Tivol
Dear James, } } Suggestions for adjustable height chairs (or modifications thereof) that } minimize or eliminate fiber shedding and static charge would be } appreciated. Thank you. } Find one made of unuppolstered (sp?) metal, or rip out the uppol- stery and replace it with wood, or cover with aluminum foil (single-use). Yours, Bill Tivol
We've been cutting tissue attached to epoxy coated with 50 nm titanium for years. We keep a special diamond for it, which does need replacing more often than knives used for tissue only. But one can get excellent sections, thick and thin, and the knife does last for a while.
Lesley Weston.
On Fri, 17 Jul 1998 edelmare-at-casmail.muohio.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I've got a couple of users who would like to pursue Pre-embedment labling of plant } tissue (trials with older knives and glass show excellent results) but are very } concerned about potential damage to diamond knife edges. So I'm collecting opinions } from experienced pre-embedment microtomists: are biological diamond knife edges } basically 'safe' from 10-20 nm gold? Such that future biological sectioning will not } suffer from any damage to the knife edge? } } Thanks. } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Supervisor } 352 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } } "WE ARE MICROSOFT. } RESISTANCE IS FUTILE. } YOU WILL BE ASSIMILATED." }
Larry, Your sad commentary could not have reached me at a more pertinent time. Today, at this very hour, a group of faculty sit and discuss the future of our only general use EM facility here at Stanford University. We have tried, as a group of concerned microscopists, to let them know how important it is to keep our facility open. Maybe they need to go on a magic carpet ride to experience the beauty and importance of our "obsolete" technology. Won't there be another mutant that we need to look at soon? JoAnn Buchanan Molecular and Cellular Physiology Stanford University School of Medicine Stanford, CA 94022
Our apologies to anyone who is having trouble reaching us. It appears that the area code change to 520 is one culprit, the reassigning of the old number to someone in Phoenix is another.
The fact that US West keeps a seperate directory assistance database from AT&T is a third, moving across town to a new building is a fourth and having a record amount of activity is a fifth.
We have taken all the steps necessary to remedy this including notices to all directory assistance sources we know of and change of address and phone to all internet sources as well.
OUR NEW PHONE IS 520-903-9366, address: 3450 S Broadmont Tucson, AZ 85713
PLEASE NOTE: do not respond to my personal email address on this missive, I am traveling and use this only sporadically. We invite all to visit Tucson to see our new facility and new applications laboratory. We will give a tour of our production facility and even let you use a phone to prove that we have one. Apologies again to anyone that has been inconvenienced.
Regards. Steve Miller Director of Sales, North America
In response to the following posting from Catherine: ================================================================= Wonder if anyone going to the 14th ICEM in Mexico? Any idea of Cancun, such as safety and traveling around? ================================================================= My wife and I are "veterans" of many trips over the years to Cancun, both for holidays and also scientific meetings, and I believe we can speak from the perspective of first hand experience, the most recent trip being about 2 years ago.
The Mexican government designated some years ago certain areas called "zona turisticas" and Cancun was one of the first (if not the first). Basically, these special areas are run from a safety and hygiene standpoint virtually like you were in the USA. The US chain hotels (e.g. Sheraton, Marriott, Hyatt, etc.) seem to be run from a safety, security, and other standpoints as if you really were in the USA. I think the other hotels in the "zona turistica" are also run to that same standard, as are also the local outside-the-hotel restaurants with the zone. We always ask for "agua purificada" and we perhaps take a few other common sense precautions, but in all our many trips to Cancun neither of us has ever suffered any problems. Hotels outside the "zone" would not be necessarily operating to that kind of standard so further precautions should be taken. The one note of caution is this: Just as there is in parts of the US a problem with salmonella in eggs, there is also the problem in Mexico so you would want to make sure that any eggs in your diet were hard boiled or scrambled well done.
So far as "traveling around", we have taken both tours as well as gone off on our own in rented cars. We have never had any problems. Everything is very reasonably priced. We have found the Mexican people to be friendly, hospitable, and genuinely appreciative of those who visit their country, perhaps in part because that part of Mexico is so dependent on tourism. From a purely touristic point of view, there are far more things to see and do than one could possibly have time for doing. The top of my list would include a day trip to Chichen-Itza, and a day trip to the neighboring island of Cozumel. If you are a scuba diver, the "wall" dive on Palancar Reef, just off of Cozumel, is one of the world's greatest dives. However, you might be better off to take a hotel for a few days in Cozumel.
Like anywhere else in the world, common sense must prevail. For example, upon arrival at the airport in Cancun, there are numerous porters to carry your luggage to a waiting taxi. There are no trolleys as there are in most airports for doing this yourself. So if you have more luggage than you can carry on your own, be prepared to take advantage of the local porters. My advice is to convert some money at the currency window right there is in the luggage arrivals area, while you are waiting for the arrival of your luggage. The rate is usually better there than at airports in the US. You should be prepared to tip the porter US $.50 per bag (or the equivalent in pesos). You will need to purchase a ticket for the taxi (really a van holding 8-10 persons) to your hotel. Within the zona turistica, it is all the same taxi rate. The cost is per person and is about US $8-$9. A group of four or more, arriving at the same time and going to the same hotel can get a better deal by reserving an entire taxi. So you should convert enough money to end up with enough pesos in order to be able to purchase your taxi tickets and give the porter a tip and also the driver a tip. If you present for payment for the taxi tickets US dollars, the rate you are given (at least that was the case in the past) is pretty crummy. Therefore the reason to get some pesos. When leaving the airport area, for the taxi ticket stand or anywhere for that matter, I would recommend keeping a close eye on your belongings. I would make this same admonition for someone arriving at JFK in NYC, but this is just common sense.
If you should arrive and one or more of your bags does not arrive, then you want to file the report with the airline before leaving the luggage area and before going through customs. If you are connecting from a different airline to make your final flight to Cancun, make sure when you check in for the Cancun flight you present your bag tags from the previous flight so make sure your bags are in fact transferred onto the Cancun flight. Other wise, with security being what it is, you could end up being separated from your checked luggage.
} From all that I have been able to learn, it really does sound like this ICEM is going to be the best ever. When I attended the MICRO 98 meeting in London a few weeks ago, the "word" was that "everyone" is going. However, there are still luxury hotel rooms available at reasonable rates so if you have not yet made your decision, now is the time to decide! One other note: Don't worry if a hotel is one or more Km from the convention center. A local bus traverses the main road along the hotel strip every few minutes and the cost per ride is roughly US$.25 , so don't let the "distance from the convention center" deter you from a particular hotel (so long as it is along the hotel strip). In any case, riding these local buses is a part of the Cancun experience you would not want to miss anyhow.
Chuck
PS: For prospective exhibitors, I am told there are still some booths (exhibit stands) available.
Disclaimer: These are my own personal opinions and recommendations and might not necessarly reflect the views of the organizers.
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
In response to the following posting from Catherine: ================================================================= Wonder if anyone going to the 14th ICEM in Mexico? Any idea of Cancun, such as safety and traveling around? ================================================================= My wife and I are "veterans" of many trips over the years to Cancun, both for holidays and also scientific meetings, and I believe we can speak from the perspective of first hand experience, the most recent trip being about 2 years ago.
The Mexican government designated some years ago certain areas called "zona turisticas" and Cancun was one of the first (if not the first). Basically, these special areas are run from a safety and hygiene standpoint virtually like you were in the USA. The US chain hotels (e.g. Sheraton, Marriott, Hyatt, etc.) seem to be run from a safety, security, and other standpoints as if you really were in the USA. I think the other hotels in the "zona turistica" are also run to that same standard, as are also the local outside-the-hotel restaurants with the zone. We always ask for "agua purificada" and we perhaps take a few other common sense precautions, but in all our many trips to Cancun neither of us has ever suffered any problems. Hotels outside the "zone" would not be necessarily operating to that kind of standard so further precautions should be taken. The one note of caution is this: Just as there is in parts of the US a problem with salmonella in eggs, there is also the problem in Mexico so you would want to make sure that any eggs in your diet were hard boiled or scrambled well done.
So far as "traveling around", we have taken both tours as well as gone off on our own in rented cars. We have never had any problems. Everything is very reasonably priced. We have found the Mexican people to be friendly, hospitable, and genuinely appreciative of those who visit their country,
perhaps in part because that part of Mexico is so dependent on tourism. } From a purely touristic point of view, there are far more things to see and do than one could possibly have time for doing. The top of my list would include a day trip to Chichen-Itza, and a day trip to the neighboring island of Cozumel. If you are a scuba diver, the "wall" dive on Palancar Reef, just off of Cozumel, is one of the world's greatest dives. However, you might be better off to take a hotel for a few days in Cozumel.
Like anywhere else in the world, common sense must prevail. For example, upon arrival at the airport in Cancun, there are numerous porters to carry your luggage to a waiting taxi. There are no trolleys as there are in most airports for doing this yourself. So if you have more luggage than you can carry on your own, be prepared to take advantage of the local porters. My advice is to convert some money at the currency window right there is in the luggage arrivals area, while you are waiting for the arrival of your luggage. The rate is usually better there than at airports in the US. You should be prepared to tip the porter US $.50 per bag (or the equivalent in pesos). You will need to purchase a ticket for the taxi (really a van holding 8-10 persons) to your hotel. Within the zona turistica, it is all the same taxi rate. The cost is per person and is about US $8-$9. A group of four or more, arriving at the same time and going to the same hotel can get a better deal by reserving an entire taxi. So you should convert enough money to end up with enough pesos in order to be able to purchase your taxi tickets and give the porter a tip and also the driver a tip. If you present for payment for the taxi tickets US dollars, the rate you are given (at least that was the case in the past) is pretty crummy. Therefore the reason to get some pesos. When leaving the airport area, for the taxi ticket stand or anywhere for that matter, I would recommend keeping a close eye on your belongings. I would make this same admonition for someone arriving at JFK in NYC, but this is just common sense.
If you should arrive and one or more of your bags does not arrive, then you want to file the report with the airline before leaving the luggage area and before going through customs. If you are connecting from a different airline to make your final flight to Cancun, make sure when you check in for the Cancun flight you present your bag tags from the previous flight so make sure your bags are in fact transferred onto the Cancun flight. Other wise, with security being what it is, you could end up being separated from your checked luggage.
} From all that I have been able to learn, it really does sound like this ICEM is going to be the best ever. When I attended the MICRO 98 meeting in London a few weeks ago, the "word" was that "everyone" is going. However, there are still luxury hotel rooms available at reasonable rates so if you have not yet made your decision, now is the time to decide! One other note: Don't worry if a hotel is one or more Km from the convention center. A local bus traverses the main road along the hotel strip every few minutes and the cost per ride is roughly US$.25 , so don't let the "distance from the convention center" deter you from a particular hotel (so long as it is along the hotel strip). In any case, riding these local buses is a part of the Cancun experience you would not want to miss anyhow.
Chuck
PS: For prospective exhibitors, I am told there are still some booths (exhibit stands) available.
Disclaimer: These are my own personal opinions and recommendations and might not necessarly reflect the views of the organizers.
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
FALL 1998 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-V2)
NASSAU COMMUNITY COLLEGE
A fifteen week, fall 1998 semester, course in Biological Transmission Electron Microscopy is being offered by the Biology Department of Nassau Community College. This is a 4 credit course offered ONE EVENING PER WEEK, Thursdays, starting at 5:30pm. Classes will begin on Sept. 3 and end on Dec. 10, 1998.
This is a "hands-on" course emphasizing biological specimen preparation, ultra-thin sectioning involving block trimming, glass knifemaking and operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III), thick and ultra-thin section mounting and contrast staining (UA and Pb citrate), grid support films (formvar, carbon), student operation of the TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs through the process of black & white photography, and electron micrograph analysis. Students will work on a chosen sample(s) with the goal of producing a portfolio of high quality TEM photomicrographs of that sample(s).
The course is widely transferrable and the cost per credit is reasonable at $86 per credit.
More information about the Bio-Imaging Center at NCC, course descriptions and syllabi, and the beginnings of a student gallery of EM photomicrographs is available at our web site. The URL is {http://www.sunynassau.edu/webpages/biology/becks.htm} .
For those without www access, the catalog description is specified below. If you have further questions, you should e-mail me directly at the address below.
Interested individuals should register early (by Aug. 10) since the course is limited to a total enrollment of ten (10) students.
Questions regarding the actual registration process can be directed to our registrar at (516) 572-7355. ________________________________________________________________________________
CATALOG DESCRIPTION BIO 221: Transmission Electron Microscopy -- 4 credits Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent. An introduction to the basic principles of transmission electron microscopy including tissue preparation, microscope (TEM) operation, black & white photography, and micrograph interpretation. The entire laboratory is devoted to the development of skills and preparative techniques involved with the operation of an actual transmission electron microscope. (3 lecture, 3 laboratory hours). Laboratory fee applies. ________________________________________________________________________________
Stephen J. Beck Associate Professor Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: {becks-at-sunynassau.edu} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
FALL 1998 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-V2)
NASSAU COMMUNITY COLLEGE
A fifteen week, fall 1998 semester, course in Biological Transmission Electron Microscopy is being offered by the Biology Department of Nassau Community College. This is a 4 credit course offered ONE EVENING PER WEEK, Thursdays, starting at 5:30pm. Classes will begin on Sept. 3 and end on Dec. 10, 1998.
This is a "hands-on" course emphasizing biological specimen preparation, ultra-thin sectioning involving block trimming, glass knifemaking and operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III), thick and ultra-thin section mounting and contrast staining (UA and Pb citrate), grid support films (formvar, carbon), student operation of the TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs through the process of black & white photography, and electron micrograph analysis. Students will work on a chosen sample(s) with the goal of producing a portfolio of high quality TEM photomicrographs of that sample(s).
The course is widely transferrable and the cost per credit is reasonable at $86 per credit.
More information about the Bio-Imaging Center at NCC, course descriptions and syllabi, and the beginnings of a student gallery of EM photomicrographs is available at our web site. The URL is {http://www.sunynassau.edu/webpages/biology/becks.htm} .
For those without www access, the catalog description is specified below. If you have further questions, you should e-mail me directly at the address below.
Interested individuals should register early (by Aug. 10) since the course is limited to a total enrollment of ten (10) students.
Questions regarding the actual registration process can be directed to our registrar at (516) 572-7355. ________________________________________________________________________________
CATALOG DESCRIPTION BIO 221: Transmission Electron Microscopy -- 4 credits Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent. An introduction to the basic principles of transmission electron microscopy including tissue preparation, microscope (TEM) operation, black & white photography, and micrograph interpretation. The entire laboratory is devoted to the development of skills and preparative techniques involved with the operation of an actual transmission electron microscope. (3 lecture, 3 laboratory hours). Laboratory fee applies. ________________________________________________________________________________
Stephen J. Beck Associate Professor Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: {becks-at-sunynassau.edu} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
I am writing to request some help please. I am a Chiropractic Physician who has taken two training courses in Enderleinian Darkfield Microscopy. I am searching for a used microscope to use for that purpose. I would like your opinion comparing the quality of the Zeiss Universal to a Leitz Orthoplan. Also, is IC optics a significant help with darkfield? Is the image from an Axioskop any better than that obtained from a Universal or ORthoplan? Finally, which type of objectives - plan Achromat. plan-Neoflour, or Plan apochromat would suffice/be recommended? Thank you very much.
Numerous micrographs of bacteria and other specimens can be found in Ruska's book: Ernst Ruska. 1980. The early development of electron lenses and electron microscopy. Translated by Thomas Mulvey. S. Hirzel Verlag (Stuttgart). ISBN 3-7776-0364-3.
A. Kent Christensen Department of Anatomy and Cell Biology, Medical Sciences II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 akc-at-umich.edu, Tel (734) 763-1287, Fax (734) 763-1166 http://www-personal.umich.edu/~akc/
-------------------------------------
On Mon, 20 Jul 1998, frank booy wrote:
} I remember seeing an image of a bacterium imaged in/by Ruska's } first microscope. } Can anyone tell me where this is to be found please? } Thanks! } } Frank Booy }
I am currently looking into a new Field Emissions Auger for our lab. I know the general differences between the Cylindrical Mirror and the Hemispherical analysers but has anyone performed a recent comparison. I run a multipurpose system doing powders, fracture surfaces, polished surfaces and anything in between. If anyone has data that they can share with me I would really appreciate it.
Thank you Tamara E. Bloomer Assistant Scientist Ames Laboratory 137 Wilhelm Hall Ames, IA 50011 (515) 294-2564
Donna Wagahoff asked: } We are in the market for a new knifemaker. Those of you with newer model } knifemakers, please let me know how you rate their performance. } Does anyone other that Leica make knifemakers? Thanks.
We (Energy Beam Sciences) manufacture both a triangular and a "Ralph" glass knifemaker. The triangular knifemaker is the descendent of the old DuPont-Sorvall knifemaker, and is designed to cut 10mm and 12mm knives (as opposed to the Leica, which is designed for 6.4mm glass). RMC also make a knifemaker, and there is another one, made in the UK, which is distributed through Agar there.
Best regards, Steven E. Slap, Vice-President
******************************** Energy Beam Sciences, Inc. The Laboratory Microwave Company http://www.ebsciences.com ********************************
First: regarding the choice between Zeiss and Leitz
The best suggestion for your application is that you try your specimen with the microscope you intend to purchase, to see which does the best job for you. I am not familiar with the term "Enderleinian" with respect to Darkfield microscopy. If you can provide a description, I may be able to comment more completely on which optics would perform best.
Secondly: regarding "IC" optics
This nomenclature refers to a different contrast technique, differential interference contrast (DIC), often called "Nomarski" (Dr. Georges Nomarski developed a method for cutting the necessary prisms which enabled the technique to become commercially viable). Since DIC is based on polarized light, it requires strain free optics; the engraving IC indicates that the optics are suitable for this technique. Making the assumption that you are working in transmitted light, a complete DIC system would require a set of objectives fitted with polarizers and DIC prisms (some systems have a single, common prism while others have a separate prism/polarizer set for each objective) as well as a matching condenser (often a turret, if you are going to do DIC with more than one objective), also fitted with polarizers and prisms.=20
These two techniques work on very different aspects of the sample:
In Darkfield, the light approaching the sample comes in at such a high angle that it cannot be collected by the objective. As a result, the background is dark. However, any feature in the sample (little grains, strings, pits, scrathes, dust) which can scatter light will do so. The scattered light is collected by the objective and used to form the image. The result: bright objects against a dark background. Since scatter is highly wavelength dependent, darkfield can also cause is extraneous color.
DIC is a bit too complex to explain completely here but basically, it detects gradients (slopes or changes)in the sample. The gradients can come from either changes in topography or changes in refractive index (ex: due to changes in concentration, gelation, etc.). In the most sensitive DIC settings, the background is usually pearly gray; one slope will be bright and the slope on the opposite side of the feature will be dark. Since you mind interprets these bright/dark cues in terms of differences in height, DIC images often appear to have increased structural or 3-dimensional information; they need to be interpreted very carefully. Sometimes adding an internal standard (ex: tiny glass beads) is helpful in understanding what is really happening. A second artifact comes from materials which respond to polarized light. Typically, they will "scramble" the DIC information. What you will see will be beautiful Pol colors, but not the true gradient information from the DIC. To test, just compare one side of the feature to the other. If the feature shows the same response on all sides (as opposed to one side being bright, the other being dark), it is probably responding to the Pol component of the system, not the DIC components.
For more on all these topics, might I suggest our book "Optimizing Light Microscopy for Biological and Clinical Laboratories"? Details are available at our website:
Thank you to those who responded to my inquiry after clean-room chairs.
We located several fitting the description in the Lab Safety Supply catalog (800-356-0783) made by a company called BioFit. The chair seats are vinyl (and look comfortable) and dissipate static by means of a brass chain fitted to the bottom of the chair, which drags on the ground. The difference between this chair and regular vinyl chairs appears to be the brass grounding chain.
No doubt other vendors carry similar products, and I have no commercial interest (of which I know) in Lab Safety Supply Co.
Thanks everyone for helping me try and build or get a microscope. My feelings are that it is better and cheaper to buy a microscope, ie - 600x from India or China for $70, (one of the plans I saw was to make a 100x for $100, but it seems outdated) but I still want to make my own eventually someday. Could someone direct me to some sources for acquiring these microscopes? Perhaps an online source or catalog. I want to use the scope to view slides with a light source from underneath, and also be able to view the surface of small objects (like within an inch in heigth or bigger).
We do not believe you would need a turbo system. Ladd sells a carbon coater (diffusion pump) that is a high vacuum system (10 to the minus 6 or 7) and we believe this would be enough for you. We could always add a turbo pump option that would add about $6,000 to the base price, which I would be happy to quote you if you are interested.
John Arnott Chairman --
LADD RESEARCH 13 Dorset Lane Williston, VT 05495
TEL 1-800-451-3406 (US) or 1-802-878-6711 (FROM ANYWHERE) fAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.msa.microscopy.com/SM/LADD
Rick Felten 07/20/98 04:25 PM I love my Stylus Color 800 and need another printer. Has anyone compared a Epson Stylus Photo 700 to a Stylus Color 800 for printing high resolution black and white images? Ric Thanks
What do you mean by IC optics: DIC (Differential Interference Contrast or Nomarski) or ICS (Infinity Corrected System (Zeiss's infinity optics)?
Infinity correction will help if you are putting anything into the light path between the objective and the Telen (projection) lens (which focusses the light rays properly for the ocular lens.
Generally apochromatic lenses have higher numerical apertures and hence higher resolving capabilities than neofluars or achromatic lenses.
As to which microscope is better for your darkfield, I think you just have to try out the different microscopes for your particular application. This may be difficult if you're trying to buy a used one, but some of the microscope companies do sell used ones.
Matt Schibler
} ---------- } From: Stephen[SMTP:smd-at-capecod.net] } Sent: Sunday, July 19, 1998 3:37 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Zeiss vs. Leitz + } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } Good day to everyone who is reading this, } } I am writing to request some help please. I am a Chiropractic } Physician } who has taken two training courses in Enderleinian Darkfield } Microscopy. I } am searching for a used microscope to use for that purpose. I would } like } your opinion comparing the quality of the Zeiss Universal to a Leitz } Orthoplan. Also, is IC optics a significant help with darkfield? Is } the } image from an Axioskop any better than that obtained from a Universal } or } ORthoplan? Finally, which type of objectives - plan Achromat. } plan-Neoflour, or Plan apochromat would suffice/be recommended? } Thank you very much. } } } } Stephen M. Driscoll }
May I suggest an HP 890C ($399). I've really been impressed with the quality when using the both the special paper and normal paper. I bought the cheaper version of this printer, the 722C ($299) for home and have been happy with it also. Both of these printers have the PhotoREt II feature and the Kodak Image Enhancements. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
Rick Felten 07/20/98 04:25 PM I love my Stylus Color 800 and need another printer. Has anyone compared a Epson Stylus Photo 700 to a Stylus Color 800 for printing high resolution black and white images? Ric Thanks
I was wondering if anyone could help me analyse some results I have obtained using acridine orange. I am using rat uterus in my experiments, and attempting to detect apoptosis in the tissue. My main problem is interpreting the changes in DNA and RNA content in the tissue; so if there are any experts out there who could possibly give me some assistance, that would be great.
Thanks
Richard
Dr Richard Stump Rm 221 Anatomy and Histology Anderson Stuart Bldg. F13 University of Sydney NSW 2006
} } Thanks everyone for helping me try and build or get a microscope. My feelings } are that it is better and cheaper to buy a microscope, ie - 600x from India or } China for $70, (one of the plans I saw was to make a 100x for $100, but it } seems outdated) but I still want to make my own eventually someday. Could } someone direct me to some sources for acquiring these microscopes? Perhaps an } online source or catalog. I want to use the scope to view slides with a light } source from underneath, and also be able to view the surface of small objects } (like within an inch in heigth or bigger). } } Danny -
Here's a list of sources of compound scopes in your price range. a 3 objective monocular with condenser will cost at least $150. You'll find several books for adult hobbiests in the Project MICRO bibliography (address below).
3-OBJECTIVE MONOCULAR COMPOUND SCOPES
There is a much greater selection in this category; follow the advice in "Microscopic Explorations", and shop around. Every major school supplier offers a selection. Here are a few possibilities: Carolina Biological Supply 800-334-5551 Edmund Scientific 800-728-6999 Educational Teaching Aids 800-445-5985 Frey Scientific 800-225-FREY Insights Visual Productions 800-942-0528 Lakeshore Learning Materials 800-421-5354 Nasco 800-558-9595 Sargent-Welch 800-727-4368 Southern Precision Instruments (dealer) 800-678-7768
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.MSA.microscopy.com/ProjectMICRO/Books.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
I am posting this message for another microscopist. Your reply direct to his email or the listserver will be appreciated. Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes *********************** www.proscitech.com.au *****
} I am looking for a staining method for looking at slime } deposits from } paper mills and biofilms in general using light microscopy. } I came } across some methods that stain bacterial capsules but none } that mentions } biofilms or slime layers (particularly levulose polymers). } } Bill van Eijk } email: whvanei-at-ibm.net
Dear Barbara, } } The chair at my microscopy bench has a naugahyde cover. Would that } help? } If one has the spiffy new synthetic-fabric lab coats (rather than the old-fashioned cotton ones), naugahyde is not a solution to the static- electricity problem. One could almost use that combination for an emer- gency HT source ;-). Yours, Bill Tivol
I'm sorry it's taken so long to summarize the responses I received to my query on how long to store tissues in glutaraldehyde fixative. The results are about even regarding whether or not to do it. Some people strongly believe that it is perfectly fine to store tissues for extended periods of time in glutaraldehyde--a much better way to store tissues than in buffer--even with preservatives and anti-.fungals. Several laboratories responded that they store tissues in glutaraldehyde all the time with no adverse effects. One laboratory mentioned that on occasion, they do see some membrane whorling in their tissues following long storage periods, but it is minor. One laboratory described very negative effects of extended storage in glutaraldehyde, such as loss of density and contrast. Another individual mentioned a paper written a few years ago that dealt with different formulations of fixatives and found that storage in a fixative called 4F1G (4% formaldehyde/1% glutaraldehyde in PO4 buffer) over a 5 year period showed no adverse effects, but storage in higher concentrations of glutaraldehyde may cause some artifacts. One laboratory reported that extended fixation in glutaraldehyde makes the tissue difficult to section due to hardening of the tissue. Additionally, they report occasional leaching of detail and less intense staining. Finally, an elegant paper from the South African Journal of Botany ( Coetzee J & van der Merwe C F - 1986. The influence of processing protocol on the ultrastructure of bean leaf cells. SA J Bot 52 (2) 95-99) describes several fixation schedules, vaying fixation times, buffers and periods in buffer wash. The authors suggest that maximum fixation times in Na-Cacodylate should not exceed 16 hr, phosphate buffers should not exceed 2 days, and PIPES-buffered glutaraldehyde fixation should not exceed 4 hours. The most accepatble fixations with all the bufferes were obtained with one hour fixation schedules. Those of us who have worked with plant material are well aware that plant material is much less forgiving than animal tissue, which may account for the varying accounts and opinions. The bottom line from just about everybody, however, is that it is best simply to not store tissues at all and immediately process them up to the block stage (though we all know that this is not always possible). I hope this information helps those who requested a summary. I greatly appreciate everybody's input (and it was great to hear from some old friends again!). Thanks again! Cheers, Laura
Laura M. Patrone, Ph.D. Wyeth-Ayerst Research Biomedical Imaging 641 Ridge Road Chazy, NY 12921 (518) 846-6318 e-mail: patronl-at-war.wyeth.com
Thank you very much for taking the time to put that info out there. I would be interested in what kind of weather to expect also if anyone has that kind of information.
Hi all, I am organizing the Materials Science Tutorials for the 1999 M&M Meeting and earlier I had asked for suggestions on topics from MAS and MSA members and other possible attendees of the conference. Below is a list of what I received in the way of suggestions. I am asking those of you who have the time to vote for up to three of the subjects. This will help me make the final decision on the topics for next year. If you have other suggestions or comments please let me know.
} 1. "How about GIF and perhaps PEELS?" } } 2. Defect recognition and analysis in crystalline materials. } } 3. Accessing and using on-line crystallogrphic databases } } 4. SEM Techniques } } 5. Optimizing the microscope for XEDS and WDS detection } } 6. XEDS imaging and interpretation, the right ways and the wrong ways. } } 7. Automation and Remote Control } } 8. More sample preparation (cross section of ALL methods) } } 9. Cross section samples with the FIB & FIB in general. } } 10. Spectrum imaging
I should note that FIB was a very popular choice and I have pretty much decided to do that one anyway, but I would still like to hear some yeahs or nays.
Thanks.
John M.
Note new Area Code (734)
John Mansfield North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 936-3352 Cellular Phone: (734) 715-2510 (Leaving a phone message at 936-3352 is preferable to 715-2510) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
Does anybody out there know how to get Leica to respond? We bought a camera system for one of our microscopes and the parts are not compatable. We have been trying to talk to our Rep. and he will not return the phone calls. I also sent an email to Leica with no response. Any ideas will be appreciated.
Marty Reed Equipment Technician Biology Department Humboldt State University Arcata CA 95521 707-826-3234 707-826-3201 FAX mmr7001-at-axe.humboldt.edu
} 9. Cross section samples with the FIB & FIB in general.
} 10. Spectrum imaging
and
EBSP
Thanks for your efforts in organizing these tutorials.
Scott
F. Scott Miller Electron Microscopy Lab smiller-at-umr.edu University of Missouri-Rolla 223 McNutt Hall voice: 573 341 4727 Rolla, MO 65409 USA fax: 573 341 6934
Can anyone tell me for sure that Tannic acid is Ok to be used in Phos buffer (at about 6.9 pH)? (for fixation after glutaraldehyde). We have always used Cac bfr, but cannot in this instance. If you just answer "yes" or "no", it is good enough. Thanks, Hildy
While on assignment, I supported a lab which did a lot of work with biofilms which formed everywhere from cooling towers for power plants to beer facilities. They used DiI (UV excitation).
For the best reference for all sorts of stains, dyes, and probes:
Molecular Probes Handbook of Fluorescent Probes & Research Chemicals,
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Does anyone have experience with the nanoplast resin? I would like to know if it possess a column contamination problem when used in the TEM or SEM. Also, are the results equal to or better than conventional resins or other resins that can tolerate some water remaining in the specimen material prior to infiltration. The intended use is for isolated starch granules. These granules are often hydrated during preparation or when used in food products. When hydrated, they are much softer and somewhat swollen so resins may be able to penetrate easier. Also, it may be of interest to compare the hydrated state with the dried morphology.
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-aux.btny.purdue.edu Purdue University or: emcenter-at-btny.purdue.edu 1057 Whistler Building West Lafayette, IN 47907-1057
by news.mtu.edu (8.8.8/8.8.8) with ESMTP id PAA25397; Tue, 21 Jul 1998 15:42:43 -0400 (EDT) Received: from mmserver.mm.mtu.edu (mmserver.mm.mtu.edu [141.219.66.30]) by mtu.edu (8.8.8/8.8.8) with ESMTP id PAA04040; Tue, 21 Jul 1998 15:42:43 -0400 (EDT) Received: from backscatter (backscatter.my.mtu.edu [141.219.67.144]) by mmserver.mm.mtu.edu (8.8.7/8.8.7/mturelay-1.2) with SMTP id PAA25134; Tue, 21 Jul 1998 15:42:41 -0400 (EDT) X-Authentication-Warning: mmserver.mm.mtu.edu: Host backscatter.my.mtu.edu [141.219.67.144] claimed to be backscatter Message-Id: {3.0.5.32.19980721153734.0097d360-at-mmserver.mm.mtu.edu} X-Sender: opmills-at-mmserver.mm.mtu.edu X-Mailer: QUALCOMM Windows Eudora Pro Version 3.0.5 (32)
A colleague at Tulane University asked that I submit this to the list. Please do not reply to me.
Cheers, Owen
} LAB SUPERVISOR (TEM/SEM) } } } Tulane University's Coordinated Instrumentation Facility seeks an } individual to create its EM facility consisting of 2 SEM's (Amray 1600T, } Jeol 820) and TEM (Philips 410). The successful candidate will possess } experience in operating within a multi-user environment serving biological } and physical sciences clientele. Experience with basic } biological/materials techniques is required including, histochemistry, } ultramicrotomy and thin foil preparation, electron diffraction, x-ray } microanalysis (EDS & WDS), photographic and darkroom procedures, digital } imagery practices. Experience is desired in the following: repair of } electron microscopes, high vacuum systems and accessory instrumentation and } basic computer skills (word process, spreadsheet, digital image } manipulation, HTML). B.S. physical/engineering required, M.S. preferred. } } Qualified applicants submit complete packet of cover letter and resume in } our office on or before August 31, 1998 to: } } Tulane University } Human Resources } Collins C. Diboll Complex } New Orleans, LA 70118 } } Tulane University is an AA/EOE. } }
Presuming a hurricane does not hit the week of the ICEM meeting, there are two words which accurately describe the weather in Cancun the first week of September: Steam Bath.
Dress (or undress) accordingly...
Larry
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Dr. Lawrence F. Allard Senior Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
Responding to the message of {n1311092614.19691-at-btny.purdue.edu} from "Microscopy Center" {emcenter-at-btny.purdue.edu} :
I have one grad student in my lab who is using it for gold labeling studies on algae. He is using it because he doesn't have to completely dehydrate the cells in organic solvents before infiltrating them.
I don't know if there is any problem with contamination of TEM column, at least none that I'm aware of........yet.
One important clue when using this resin, is blocks tend to come out VERY hard if you use the recommended amount of catalyst B-52, such that you can't even trim it. We use 1/4 the amount specified in the instructions that come with the resin. So advise you try a few curring runs of blocks with no sample just to work out the amount of catalyst to use such that you get blocks soft enough to trim and cut.
A thread on nanoplast ran on this listserver in March. I compiled them into a file and will send it to you off-line as an attachment.
Good luck!
Gib
} Does anyone have experience with the nanoplast resin? I would like to know } if it possess a column contamination problem when used in the TEM or SEM. } Also, are the results equal to or better than conventional resins or other } resins that can tolerate some water remaining in the specimen material prior } to infiltration. } The intended use is for isolated starch granules. These granules are } often hydrated during preparation or when used in food products. When } hydrated, they are much softer and somewhat swollen so resins may be able to } penetrate easier. Also, it may be of interest to compare the hydrated state } with the dried morphology. } } Debby Sherman, Manager Phone: 765-494-6666 } Microscopy Center in Agriculture FAX: 765-494-5896 } Dept. of Botany & Plant Pathology E-mail: sherman-at-aux.btny.purdue.edu } Purdue University or: emcenter-at-btny.purdue.edu } 1057 Whistler Building } West Lafayette, IN 47907-1057 } } } .
Gib Ahlstrand, Minnesota Micoscopy Society Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
"Theory and practice are the same in theory, but different in practice."
May I correct Steven Slap's comment on the Leica knifemaker - +ACI-as opposed to the Leica, which is designed for 6.4mm glass+ACI-.
The Leica EM KMR2 is designed to produce glass knives using a balanced break technique from 6.4, 8.0 and 10.0 mm glass. If you would like more details - contact the friendly folks at Leica.
Philip Hyam Product and Marketing Manager - Sample Preparation Leica Microsystems Canada
I've had similar experiences in my area. You might try to find your rep's boss's name, or try the management chain in headquarters at Deerfield IL (800-248-0123).
Leonard R. Corwin Fort Dodge Animal Health Princeton, NJ 08543-0400
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Does anybody out there know how to get Leica to respond? We bought a camera system for one of our microscopes and the parts are not compatable. We have been trying to talk to our Rep. and he will not return the phone calls. I also sent an email to Leica with no response. Any ideas will be appreciated.
Marty Reed Equipment Technician Biology Department Humboldt State University Arcata CA 95521 707-826-3234 707-826-3201 FAX mmr7001-at-axe.humboldt.edu
by imo21.mx.aol.com (IMOv14_b1.1) id RYPZa12330; Tue, 21 Jul 1998 17:27:06 -0400 (EDT) Message-ID: {34b297c0.35b507ac-at-aol.com}
In a message dated 98-07-21 12:18:37 EDT, mmr7001-at-axe.humboldt.edu writes:
{ { Does anybody out there know how to get Leica to respond? We bought a camera system for one of our microscopes and the parts are not compatable. } }
Marty,
I agree with Leonard Corwin, Leica Customer Service at (800) 248-0123 should be able to help you. I don't know which e-mail address or web page you tried, but your request may be floating in cyberspace somewhere in Germany.
Did you purchase from Leica directly or through one of their regional dealers? Territories and reps change, and it's possible your area has been reassigned. Anyway, just give Customer Service your Zip code, and they can take it from there.
Hope this helps! Bob ***************************************** Robert (Bob) Chiovetti rchiovetti-at-aol.com E. Licht Company / 1-800-865-4248 Colorado/Utah/Wyoming/Arizona/ New Mexico/West Texas Representing Leica Since 1967 *****************************************
I have used LR Gold for embedding samples destined for EM immunocytochemistry on many occassions with no trouble. In my latest embedding, the blocks are not behaving in a typical fashion. Everytime I go to section them, they pull the water out of the boat and prevent thin sectioning. I can cut 0.5 um LM sections with effort and the sections look okay. Has anybody else had this experience and, more importantly, do you know how to get around it? TIA, tom phillips
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211 (573)-882-4712 (voice) (573)-882-0123 (fax)
} From all the fixations I did many years ago with Tannic acid after glutaraldehyde, my short answer is
YES.
P.S.: Try to use the lightest molecular weight mixture of tannic acids you can find. Ones from Turkish nutgalls, not Chinese nutgalls. Mallinkrodt #1763 or #1764 are what I used.
} ---------- } From: HILDEGARD CROWLEY[SMTP:hcrowley-at-du.edu] } Sent: Tuesday, July 21, 1998 9:43 AM } To: postmessage } Subject: Tannic acid+Phos bfr????? } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } Hi, } } Can anyone tell me for sure that Tannic acid is Ok to be used in Phos } buffer (at about 6.9 pH)? (for fixation after glutaraldehyde). We have } always used Cac bfr, but cannot in this } instance. If you just answer "yes" or "no", it is good enough. } Thanks, } Hildy }
Tom, you may have already considered these solutions. They apply to = sectioning: 1. maintain a lower than normal water level in the boat, so the level is = slightly concave but maintains contact with the edge of the knife; and = 2. use a faster cutting speed. These are effective in reducing that = notorious water "pulling" by the LR's. Hank Adams Cell Biology Integrated Microscopy Core Baylor College of Medicine One Baylor Plaza Houston, Tx 77030
} The sample is embedded in a Spurr's resin. I am currently using an } Iodine solution but I was wondering if there was anything better? } } Robert } } Robert.Dickson-at-kcl.fi
You should be able to stain Spurr's with PAS provided the 1% periodic acid step is long enough, e.g. 30 minutes. If you want the complete procedure get back to me.
The staining should be a lot more intense than with Iodine, but other cell components may also stain up. Presumably you are looking at plant tissue? Some cell wall polysaccharides are likely to stain, also some phenolics. Nuclei may also stain - see Litwin, JA & Kasprzyk, JM (1974) Acta Histochemica, vol 50, pp 222-227 "Some remarks on the PAS reaction in semi-thin sections of Epon-embedded tissues". If your tissue was embedded in methacrylate resin or similar you could do a control of sorts by digesting the section with amylase before staining, but this may not work on Spurr's.
O'Brien and McCully recommend aniline blue black as a counterstain: 1% aniline blue black in 7% acetic acid for 10 min at 50 deg.C, rinse in 7% acetic acid. (Yes, I've got a copy of their out-of-print book, and it's not for sale).
Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department School of Medicine University of Auckland Private Bag 92019 Auckland New Zealand
Philip Hyam wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } May I correct Steven Slap's comment on the Leica knifemaker - +ACI-as opposed to the } Leica, which is designed for 6.4mm glass+ACI-. } } The Leica EM KMR2 is designed to produce glass knives using a balanced break technique } from 6.4, 8.0 and 10.0 mm glass. If you would like more details - contact the } friendly folks at Leica. } } Philip Hyam } Product and Marketing Manager - Sample Preparation } Leica Microsystems Canada
Right Philip! But then again, if you are on a limited budget, the "old" LKB Knife Makers still do a great job for 6,4 mm glass. They can be reconditioned (by us) at a very reasonable cost and reconditioned units are available. Markus F. Meyenhofer Microscopy Labs micro-at-mail.superlink.net
I really dislike "me too" postings, but I have a similar complaint to = Marty's. I would like to suggest to any Leica personnel out there to = make your Head office aware of the need for greater accessibility of = your applications dept. This means no disregard to regional offices, but = it is often helpful to talk to people closely involved in the = development of a particular instrument.
James Wesley-Smith Electron Microscope Unit University of Natal,=20 Durban, South Africa
----------
Does anybody out there know how to get Leica to respond? We bought a camera system for one of our microscopes and the parts are not = compatable. We have been trying to talk to our Rep. and he will not return the phone calls. I also sent an email to Leica with no response. Any ideas will = be appreciated.
Marty Reed Equipment Technician Biology Department Humboldt State University Arcata CA 95521 707-826-3234 707-826-3201 FAX mmr7001-at-axe.humboldt.edu
I have just heard from Prof. Sitte that, following his retirement, this year's workshops will be the last. So, having been a speaker in them since 1986, I thought I would say a few words.
For those who do not know, the workshops have been running in Seefeld (Tirol) since 1973 and, before that, in the University of Homburg, Germany. Over 2,000 biomedical and materials scientists and technicians from around the world have participated in them.
The workshops are renowned for their technical content, social intercourse, gastronomic experience, cultural interest and scenic beauty (being on a high plateau in the Alps). Somehow, Sitte has perfected the art of being the gracious host who combines long hours of learning with enjoyment.
The courses are taught by various experts in their fields: some are the developers of ultramicrotomy and cryo-equipment, some are immuno experts and others have made fundamental contributions to the field.
The details I have are given below, although the costs may be negotiable! Students, those from former Eastern Europe and Third World countries are HALF price. Further (definitive!) details are available from Prof. Sitte: FAX Austria (0043 from UK) 5212 22 16 22 Mail address: Prof. Sitte, Reitherspitzstrasse 166, A-1600 Seefeld in Tirol, Austria. _______________________________________________________
The language of these workshops is English (Workshop 64, in the German language, runs from 14 to 29 October).
Workshop 63 E-Bio & E-Mat (Biomedical and Materials) Introductory sectioning workshop (without cryo or immuno) Begin 9.30 am 24 Sept. 98, finish 9 pm 27 Sept. 98 (course fee AS 7,000).
Workshop 63 F-Bio Cryoultramicrotomy, cryomethods, immuno-gold-cytochemistry Begin 9.00 am 4 Oct, finish 9 pm 8 Oct (course fee 13,000).
Workshop 63 A-Bio (combined E & F workshops, course fee AS 16,000). Workshop 63 A-Mat (combined E & F workshops, course fee AS 14,000).
Workshop B-Bio Cryofixation, freeze substitution, freeze drying, progressive lowering of temperature technique and low temperature embedding (without cryoultramicrotomy and immuno) Begin 9.00 am4 Oct, finish 9 pm 8 Oct (course fee AS 8,000).
Workshop 63 F-Mat Cryoultramicrotomy, diamond knife sectioning and preparation of hard/inhomogeneous samples. Begin 9.00 am 27 Sep, finish 9 pm 2 Oct (course fee AS 13,000).
Hi everyone, I am looking for a course on basic SEM theory, operation and hands on training. The course should not last longer than 5 days and should take place during this year. All suggestions are welcomed. Thanks! Gabriel Rojano rojanog-at-macom.com Reliability Engineering AMP- M/A-COM
I am curious as to the best way to determine the thickness of the car= bon evaporation technique being deposited on the substance to be analysed= . It would be beneficial to know the method by which a knowledge of thi= s informationcan be attained within a few Angstroms. Joshua McCaig. (mccaigjm-at-corning.com)=20 --=20 MZ=90
Hi listers, I have to fix 18.5 dpc mouse embryo in formalin or paraformaldehy for immunostaining. Does anyone out there knows the procedure for paraffin processing and embedding? We had tried several times unsuccessfully. I like to know specifically how long and in what temperature those huge embryo should be fixed (fixative penetration issue), and keep the antigenicity as well. Thanks.
******************************************************************* To see what is in front of one's nose requires a constant struggle.
George Orwell
Dorothy Zhang Harvard School of public Health Building 2, CVLAB 677 Huntington Ave, Boston, MA 02115 Phone# 617-432-6981 Fax# 617-432-2980
You may leave the water at slightly lower level even though you may not see the sections right after cutting.
Good luck !
Ming
On Tue, 21 Jul 1998, Tom Phillips wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I have used LR Gold for embedding samples destined for EM } immunocytochemistry on many occassions with no trouble. In my latest } embedding, the blocks are not behaving in a typical fashion. Everytime I } go to section them, they pull the water out of the boat and prevent thin } sectioning. I can cut 0.5 um LM sections with effort and the sections look } okay. Has anybody else had this experience and, more importantly, do you } know how to get around it? TIA, tom phillips } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211 } (573)-882-4712 (voice) } (573)-882-0123 (fax) }
*********************************************** * Ming H. Chen, PhD * * Medicine/Dentistry Electron Microscopy Unit * * #1074B Dentistry Pharmacy Building * * University Of Alberta. * * Edmonton, Alberta, Canada T6G 2N8 * * * * Visit My Page At: * * http://www.ualberta.ca/~mingchen * ***********************************************
To the lung experts, What do you do with floating lung tissue (} 2mm) that is fixed by immersion. The options are: 1)leave in a refrig overnight until they sink (swirling did not work). 2)place in a vac (15 psi) while still in the fix. 3)process the floating samples hoping they eventually sink. I am currently in the frig (opting for #1) but any hints or suggesstions would be welcome. Thanks.
We have an elderly, incomplete Leitz manual microtome about the place here. Although a possible candidate for a boat anchor, weighing perhaps 50 lb, it contains wonderful German machining and has many mysterious knobs that invite twiddling. It appears to increment (click!) after doing a slice. It is under consideration for use to section plant tissues for light microscopy at medium magnification to look at fungal pathogens at the cell level. (Not by me - I do chemical microscopy so I rarely slice.) The unit is about two feet long, resembles an infertile cross between a lathe and a bologna slicer, and the carriage is connected to a wide ribbon apparently to smooth the slicing action. It is painted gray and might plausibly date from the 1960s. It appears that the sample to be cut is intended to be clamped below, while there are clamps spanning a rather large gap (about 8 inches) which could plausibly hold a blade, although the span seems rather large (flexion). There is nothing resembling a blade holder with the microtome.
To get to the point: Can anyone supply a photocopy of a manual or pertinent parts of a manual describing the function of the parts? (fax 609-275-5239) Or perhaps be prepared to discuss how this works by telephone? Do you think it likely to be worth having a blade holder fabricated, or should we post a notice to the boat owners?
Leonard R. Corwin Fort Dodge Animal Health Cyanamid Agricultural Research Center PO Box 400 Princeton, NJ 08543-0400 609-716-2278; fax 609-275-5239 corwinl-at-pt.cyanamid.com
} 2) place in a vac (15 psi) while still in the fix.
I'd reccommend this route, specifically as follows:
Take a 2-hole stopper (or make one if you can't find one), and put the wide end of a cut-off pasteur pipette, or a length of glass tubing, into one hole. Choose a size of stopper which will seal the top of the container you're using for the fix. Use tygon tubing to connect the glass tube to house vacuum, or connect it to a similar device made with a one-hole stopper which fits onto a side-arm flask--this will prevent any oil in the line from getting to the sample. Turn on the vacuum, seal the fixing container with the two-hole stopper, place your thumb over the empty hole until the residual gas emerges from the tissue, then release the pressure. Repeat until the gas has escaped from the tissue. Note that the one-hole and two-hole stoppers can be interchanged, so if you have to hold the stopper onto the fixing container, it can be better secured if you don't have to worry about rocking the device with your thumb. Good luck. Yours, Bill Tivol
In this paper Professor Lindsay of ASU characterizes biomolecular nano-wire electron transfer using scanning probe microscopy. This characterization requires the samples are held in an environment that is free of moisture and molecular oxygen.
____________________________________________________________________ ____________________________________________________________________ George Sibbald, President Molecular Imaging Corporation; SPM Technology Leaders for Environmental / In Situ" SPM 9830A South 51st Street, Suite A124 Phoenix, AZ 85044, USA Phone(602)753-4311, Fax(602)753-4312 http://www.molec.com/
I have cross-sectioned through gold fibers in animal tissue in the past, using a diamond knife. I did not see any problem or increased "knife marks" from these very thin ( {30 um diam.) fibers. However, I have also sectioned particles in tissue such as cobalt or titatium and one can clearly see in the photographs that there is no "knife mark" prior to hitting the particle but a HUGE one continuing beyond it, of the same thickness as the particle. By the end of sectioning a few such particle samples, that area of the knife is basically useless.
In my experience the gold doesn't dull the knife any more than animal skin, but I've never done plant tissue so I don't know how that relates.
Karen -- Karen Zaruba, kszaruba-at-mmm.com BioMaterials Technology Center 3M Center Bldg. 270-1S-01 St. Paul, MN 55144
*The opinions above are my own, not necessarily my employer's*
I'm in dire need of a part for my Technics Hummer III sputter coater (the plastic nut at the base of the specimen pedestal has stripped threads and cannot hold a vaccum). I can't find a current address or phone number anywhere. Can someone please reply direct??
TIA and cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
MICHAEL DELANNOY wrote: } } To the lung experts, } What do you do with floating lung tissue (} 2mm) that is fixed } by immersion. The options are: } 1)leave in a refrig overnight until they sink (swirling did not work). } 2)place in a vac (15 psi) while still in the fix. } 3)process the floating samples hoping they eventually sink.
{snip}
I never thought about it but I guess I am an expert (24 years)in TEM of lung. You did not specify whether embedding will be for LM or EM. If plastic embedding for EM, } 2mm is too thick and they should be sliced to not exceed 2mm. Other dimensions are not important as long as they will fit in an upside down Beem capsule with the pyramid end cut off.
First put them under vacuum in fix to pull out the air (the tissue will still be at the surface while in the vac) which will optimize contact of fix with tissue (I interpret your term immersion to indicate that the tissue was not first perfusion fixed or insufflated with fix).
Then they can go in the fridge or stay at room temp and should sink.
They will definitely sink during subsequent steps with osmium and acetone dehydration for TEM.
My experience does not extend to paraffin processing of lung.
Tom Christensen Pathology Boston University Medical Center
Try decreasing the water level in your boat (surface will be concave; make sure the edge is wet--if you have trouble with wetting, email back.)
Also, you can decrease the clearance angle of your knife.
Finally you might speed up the cutting stroke.
Be careful in aligning, that you don't wet the block face or the back of the knife. Don't allow the knife to stop directly at the block face; always go through a cutting stroke when checking distance, etc. The water has a way of "jumping" onto the hydrophillic block.
Good luck.
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
} I am curious as to the best way to determine the thickness of the carbon } evaporation technique being deposited on the substance to be analysed. } It would be beneficial to know the method by which a knowledge of this } informationcan be attained within a few Angstroms. Joshua McCaig. } (mccaigjm-at-corning.com) } -- } MZ
By using a Digital Thickness Monitor you should be able to measure carbon depositon within a few Angstroms. Since carbon is directional, it is important to locate the sensor close to the specimen. In our carbon substrate production we are able to replicate deposition within 8 to 10 Angstroms. For this it is vital that you duplicate the time, amperage, vacuum and location of the substrates. It is also important that you are consistent in your choice of carbon or graphite.
Hope this helps, John Arnott
Disclaimer: Ladd Research sells Vacuum Evaporators, Digital Thickness Monitors and substrates.
LADD RESEARCH 13 Dorset Lane Williston, VT 05495
TEL 1-800-451-3406 (US) or 1-802-878-6711 (FROM ANYWHERE) fAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.msa.microscopy.com/SM/LADD
Mike, Please send me your web site address in order that I might see what you have to offer. Thanks again for the info on Leitz, Zeiss, and Olympus. Stephen
I have noticed this same problem with LR White when using a diamond knife. My conclusion was that after prolong cutting the knife edge becomes coated with plastic residue, and this causes the sections to bind up, causing a series of cutting problems (block wetting, sections not coming off the edge, compression). Try cutting the block with a glass knife, if this is the problem the block will cut fine, if not....back to the drawing board.
William R.McManus Supervisor Electron Microscope Facility Department of Biology Logan UT 84322-5305 billEMac-at-cc.usu.edu 435-797-1920 Fax 435-797-1575
Sometime ago, I had a Hummer III. Memory at this point could be failing, but I think the nut at the pedestal base (the one which could be loosened to raise/lower the specimen on mine) was a "bonnet" nut - either an electrical feed through bushing or a plastic tube fitting nut. Such a replacement would be common and cheap from a building supplies vendor.
Again, if memory serves.... Hummer is (was?) sold by Anatec(k?), in the Washington D.C. vicinity.
_Woody_
shAf wrote: } } I'm in dire need of a part for my Technics Hummer III sputter coater } (the plastic nut at the base of the specimen pedestal has stripped } threads and cannot hold a vaccum). I can't find a current address or } phone number anywhere. Can someone please reply direct?? } } TIA and cheerios, shAf } } {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} } Michael Shaffer, R.A. - ICQ 210524 } Geological Science's Electron Probe Facility - University of Oregon } mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
-- de Woody, WB4QXE
Work: Electron Microscopist/Microanalysist Balance: Ham radio "homebrewing", computers , shade tree mechanic "the Gravel Garage".
On the www page: Scanning Electron images and Ham Radio Homebrewing stuff. http://www.geocities.com/capecanaveral/3722 .
We run courses "in house", in your own laboratory on your own equipment, = on all aspects of electron microscopy. With the world the way it is this mea= ns we spend more than 85% of our time with SEM on - specimen preparation, instrument optimisation, x-ray analysis, maintenance and consultancy. =
Please take a look at our web site for more information, we are able to tune a course to any requirement.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
On Wed, 22 Jul 1998 12:31:20 -0400 (EDT) MICHAEL DELANNOY {delannoy-at-welchlink.welch.jhu.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } To the lung experts, } What do you do with floating lung tissue (} 2mm) that is fixed } by immersion. The options are: 1)leave in a refrig overnight until } they sink (swirling did not work). } 2)place in a vac (15 psi) while still in } the fix. } 3)process the floating samples hoping } they eventually sink. } I am currently in the frig (opting for #1) but any hints or } suggesstions would be welcome. Thanks. } } Mike D }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
A friend had this problem with plant material. She kept the samples submerged under a piece of wire mesh.
Michael Shaffer asked: } I'm in dire need of a part for my Technics Hummer III sputter coater } (the plastic nut at the base of the specimen pedestal has stripped } threads and cannot hold a vaccum). I can't find a current address or } phone number anywhere. Can someone please reply direct??
The Hummer range of sputter coaters is manufactured by Anatech Ltd in Springfield, VA. The phone number is 800-752-7629 and the fax number is 703-941-8077. George Barr, the President, can certainly help you. His e-mail address is gbarr-at-anatechltd.com
Best regards, Steven Slap
******************************** Energy Beam Sciences, Inc. The Laboratory Microwave Company http://www.ebsciences.com ********************************
6621 Electronic Drive Springfield, VA 22151 (800)-PLASMA-9 or 703-941-8860 FAX: 703-941-8077
Her ext. is #104.
She fixed my Technics Hummer V promptly and will assist in over the phone consultations.
Ginger
Ginger Baker EM Lab Manager OMS Secretary/Treasurer Research Dept., OCOM 1111 W. 17th St. Tulsa, OK 74107 Phone: (918) 561-8232 FAX: (918) 699-8629 http://osu.com.okstate.edu/dept/research/content/gbaker.htm lizard-at-osucom-fs02.ocom.okstate.edu
-----Original Message-----
I'm in dire need of a part for my Technics Hummer III sputter coater (the plastic nut at the base of the specimen pedestal has stripped threads and cannot hold a vaccum). I can't find a current address or phone number anywhere. Can someone please reply direct??
TIA and cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I have an old Vickers-Armstrong hardness tester. To jog your memory, it has a foot pedal load actuator and a microscope mounted on a hinged bracket, which is moved in place over the indentation for measurement. Anyway, the eye piece tube and the attached micrometer ocular has been damage by an unknown user; this is a multi-user tester. Can anyone tell me who is in the business of servicing such a beast, Canadian rep. if possible. This is a very good, solid hardness tester and I would like to keep it. They don't make them like this anymore.
Thanks in advance. David Chow National Research Council Canada David.Chow-at-NRC.CA
Microscopy/Microscopy Education also specializes in customize, on-site courses. We have over two dozen consultants here in the US who have experience on a wide variety of electron microscopes and related analytical techniques.
For more information, see our website: { {http://www.MME-Microscopy.com/education}
In regards to the answer concerning the Technics address and phone number, I hit "reply to all" instead of "reply" so email may bounce on the listserv. Sorry all.
Ginger
You need to contact Lisa Jackson at Anatech, Ltd.
6621 Electronic Drive Springfield, VA 22151 (800)-PLASMA-9 or 703-941-8860 FAX: 703-941-8077
Her ext. is #104.
She fixed my Hummer V promptly and will assist in over the phone consultations.
Ginger Baker EM Lab Manager OMS Secretary/Treasurer Research Dept., OCOM 1111 W. 17th St. Tulsa, OK 74107 Phone: (918) 561-8232 FAX: (918) 699-8629 http://osu.com.okstate.edu/dept/research/content/gbaker.htm lizard-at-osucom-fs02.ocom.okstate.edu
Ginger Baker EM Lab Manager OMS Secretary/Treasurer Research Dept., OCOM 1111 W. 17th St. Tulsa, OK 74107 Phone: (918) 561-8232 FAX: (918) 699-8629 http://osu.com.okstate.edu/dept/research/content/gbaker.htm lizard-at-osucom-fs02.ocom.okstate.edu
I need information about video printers for printing SEM images and need = to contact sales representatives from various companies. Please contact = me at 217-782-0898. Thanks. Donna Wagahoff SIU School of Medicine PO Box 19230 Springfield, Il 62794-1220
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} This prompts me to ask the obvious question. Does anyone have a foolproof } indicator to check the activity of an osmium solution, ideally without } processing tissue and viewing in the microscope? } } I 'm sure at some time we have all picked up a bottle of expensive osmium } and thought do I use it or make fresh up. This happened to me recently and I } tried soaking some osmium into a piece of cocktail stick, which appeared to } darken, but I was wrong. } } Malcolm Haswell
Malcolm, I would put a drop of corn oil (maize oil on your side of the puddle) on a slide and some of the osmium solution in a small dish (size so that the slide acts as a lid for the dish). If the osmium is good, vapors from it will blacken the oil droplet. (Any polyunsaturated oil will do.) Phil
&&& Illigitimi non carborundum &&&&&&&& Philip Oshel Station A PO Box 5037 Champaign, IL 61825-5037 (217) 355-1143 oshel-at-ux1.cso.uiuc.edu *** looking for a job again ******************
Steve Limbach Associate Researcher Bock Research Lab. 1525 Linden Dr.
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TEL 608 263-2582 FAX 608 262-4570 EMAIL slimbach-at- facstaff.wisc.edu
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Hi, All.
I am looking for a secondary detector tube for JEOL JSM 35 in a good condition. Willing to pay a fair price for it. Please reply at my email address. Many many thanks for your anticipated co-operation.
Jitu Shah
Dr.Jitu Shah H.H. Wills Physics Laboratory, University of Bristol, Royal Fort, Tyndall Avenue, Bristol BS8 1TL. UK email: jss-at-siva.bristol.ac.uk Tel: 44 117 9288719 Fax: 44 117 9255624
Steve Limbach Associate Researcher Bock Research Lab. 1525 Linden Dr.
UW-Madison Madison, Wisc. 53706
TEL 608 263-2582 FAX 608 262-4570 EMAIL slimbach-at- facstaff.wisc.edu