Dorota, I also have a Reichert ultracut and it does fine with very large thick sections. Knives tend to suffer however. Depending on your sample, you may find that you'll need a diamond histo knife. Glass however will also work . MG Engle Electron Microscopy & Imaging Facility University of Kentucky
Have you been experiencing trouble with vibration before now?
I'm on the 4th floor of my building, which has a lot of activity during the day. In my lab there are 4 sectioning stations with ultramicrotomes lined up in the same room, sited against the same wall. Three are on marble tables, one is on a 'floating' table.
Hands down, the N2-damped table beats out the marble tables.
For any critical work, the microtome on the floating table is usable at any hour of the day, while those on marble tables usually must be used after-hours, after traffic in the building has eased. The vibration in the floor is easily visible in the water's surface on any of the 3 marble tables; it is damped out in the N2-table. Well worth the investment.
Good luck! Ann Lehman EM Facility Mgr Trinity College Hartford, CT v 860-297-4289 f 860-297-2538 e ann.lehman-at-exchange.cc.trincoll.edu
-----Original Message----- } From: Gerroir, Paul J [mailto:Paul.Gerroir-at-crt.xerox.com] Sent: Friday, January 29, 1999 2:44 PM To: Microscopy-at-Sparc5.Microscopy.Com
January 29, 1999
Fellow Microscopists, I am soliciting your comments on what you consider the best approach to damp vibrations when using a light microscope. We are in the process of redesigning our light microscopy/image analysis work area and have a choice between a conventional marble table and a Newport BenchTop vibration isolation system (pneumatic). Which should it be? Are there other possibilities to consider?
Thanks for a moment of your time. Paul
Paul J. Gerroir Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
We do a lot of extremely sensitive micromanipulation work here with the LM and have a few very effective anti-vibration systems.
We placed a heavy slab (approx. 400lb) 2' x 3' of Boiler Plate Steel on top of about 150 tennis balls on a well supported bench. The steel is well-protected and finished to make a clean, safe work area. Total cost was about $250. Cdn.
If you'd like more details, you can contact me off-line.
Karen Rethoret Microscopy Lab York University Toronto, Ont. 416-736-2100 x33289
On Fri, 29 Jan 1999, Gerroir, Paul J wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } January 29, 1999 } } Fellow Microscopists, } I am soliciting your comments on what you consider the best approach to damp } vibrations when using a light microscope. We are in the process of } redesigning our light microscopy/image analysis work area and have a choice } between a conventional marble table and a Newport BenchTop vibration } isolation system (pneumatic). Which should it be? Are there other } possibilities to consider? } } Thanks for a moment of your time. } Paul } } Paul J. Gerroir } Microscopy } Materials Characterization } Xerox Research Centre of Canada } 2660 Speakman Drive } Mississauga, Ontario L5K 2L1 } } Phone: (905) 823-7091, ext. 216 } FAX: (905) 822-7022 } e-mail: paul.gerroir-at-crt.xerox.com } }
Research Opportunities Mount Sinai School of Medicine is a leader in medical research and education. The establishment of our new Microscopy Center has created opportunities for experienced Microscopists with a BS/BA or MS in Biology or Life Sciences. All applicants should have excellent organizational and communication skills, an understanding of basic laboratory procedures, and the ability to manage a large and varied workload.
Electron Microscopist The successful candidate will participate in ultrastructural studies of various biological systems. Qualifications include at least 2 years of experience in routine electron microscopy procedures (TEM, SEM), ultramicrotomy, immunogold labelling, specimen preparation, photographic darkroom work, and routine maintenance of equipment. Individuals with immunofluorescence and confocal microscopy experience are desirable. Code: EM
Light Microscopist The successful candidate will participate in biomedical studies that use a variety of advanced light microscopic techniques. Duties will include maintaining equipment, instructing users in equipment operation, and sample preparation. Qualifications include at least 2 years of experience in fluorescence and confocal microscopy, immunofluorescence labelling, in situ hybridization, digital imaging and analysis, cell culture, and histological techniques. Strong computer skills are essential. Code: LM
We offer a salary commensurate with experience and excellent benefits. For consideration, please mail your resume, which must indicate code for position of interest, to:
Scott Henderson, Ph.D., Director, Microscopy Center, Box 1007, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029-6574
http://www.careermosaic.com/mountsinai
We are an equal opportunity employer fostering diversity in the workplace.
______________________________
Scott Henderson, Ph.D. Director of Microscopy, Mount Sinai School of Medicine, Dept. of Cell Biology & Anatomy, Box 1007, One Gustave L. Levy Place, New York, NY 10029-6574
Paul, we use a combination of marble tables and pneumatic shock absorbers on our microscopes. We have been very happy with the results. One of our microscopes has the bench top vibration table which is also very good.
At 02:44 PM 1/29/99 -0500, Gerroir, Paul J wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi Doug: Try this paper. You will have to adapt the protocol to fit your needs. If you have any questions please feel free to call.
Coller, Barry S., Kutok, J.L., Scudder, L.E., Galanakis, D.K., West, S.M., Rudomen, G.S., Springer, K.T. . Studies of Activated GPIIb/IIIa Receptors on the Luminal Surface of Adherent Platelets: Paradoxical Loss of Luminal Receptors When Platelets Adhere to High Density Fibrinogen. J. Clin. Invest. (1993) Vol. 92, pp. 2796-2806.
Doug Matthews wrote:
} Hi everyone, } } I'm interested in doing some immuno work with the SEM. Basically } tagging colloidal gold onto a surface antigen on cultured cells. I've got } some old information and papers but as for up-to-date methodologies, I'm a } little sketchy. Does anyone have any good review papers on the use of immuno } surface markers w/ SEM (preferably colloidal gold?) Nothing too fancy. In } case it helps, I'm specifically looking at phosphotidylserine exposed on the } outer membrane leaflet during apoptosis in a cultured line of CML cells. } Thanks in advance. } } Doug Matthews } Providence College } }
-- Regards, Gregory Rudomen Technical Specialist University Microscopy Imaging Center State University of New York at Stony Brook 516-444-3126 Greg-at-umic.sunysb.edu *************************************** Standard disclaimer: The opinions expressed in this communication are my own and do not necessarily reflect those of the University Microscopy Imaging Center. ***************************************
Hi! Thanks to all of you who responded to my posting. All suggestions are very helpful. I forgot to add that the tissue (lung from rat) is embedded in Epon/Araldite. It is not cryosectioning. Thanks again Dorota
I am submitting a request for a faculty member who is not a member of the list. The researcher wishes to locate, for sale, antibodies and/or conjugated antibodies for Salmonella, Clostridium and Campylobacter.
Any replies may be directed to my e-mail address and not to the list.
With Best Wishes,
Bill Monroe
Bill Monroe EM Center Mississippi State University (601)-325-3019 Lab Fax 325-0246
Greetings, I'm looking for a conductive epoxy (for materials science use) in which I can embed metal samples, sand and polish, and then look at them in a field emission SEM. I have seen this used at other facilities, so I know it exists. I found something at Electron Microscopy Sciences that I thought might work, but the product has been discontinued. Does anyone know of an epoxy that can be used for the above application?
Any replies can be directed to me personally.
Thank you, Kevin Croat tkc-at-howdy.wustl.edu Dept. of Physics Washington University in St. Louis
Thanks to all those who responded to my question about resin problems. I got a number of good suggestions, mostly related to storage (most people thought storing resins in the fridge was unnecessary and probably a bad idea), use of accelerator (several people suggested that I switch to BDMA or add DMP-30 to all infiltrating steps) or water contamination in any of the components (several people suggested replacing all resins). We will definitely be following up. Many thanks for taking the time to reply.
Marie
Dr. Marie E. Cantino Dept. of Physiology and Neurobiology University of Connecticut Storrs, CT 06269-2131 Phone: 860-486-3588 Fax: 860-4861936
The Microscopy Society of America (MSA) and the MSA Technologists' Forum are the sponsors of the Professional Technical Staff Awards (PTSA) to provide assistance on a competitive basis to full-time professional staff who submit papers for presentation at Microscopy and Microanalysis '99 (M&M '99). The deadline (15 February 1999) is fast approaching! Eligible applicants: you are encouraged to submit an abstract and supporting documentation. Managers: you are encouraged to support eligible staff members in this effort. Please read the following, taken from the M&M '99 Registration Bulletin, for more information.
It is the intent of this award to stimulate attendance at M&M '99 for professional technical staff who ordinarily might not participate in the meeting, and to encourage employers to support their staff in professional activities. Awardees will be selected based on the quality of an abstract submitted for presentation at M&M '99. The applicant must be the first author of the submitted abstract. Applicants must be full paid-up members of MSA at the time of application. The awards consist of free full registration for the meeting, a copy of the Proceedings and the Sunday evening social event. MSA will reimburse awardees up to $600 for travel, lodging and other expenses. There will be a maximum of four awards given. Successful applicants must present their abstracts personally at M&M '99 in order to receive the award. They are expected to attend and participate in the entire meeting. Former winners will not be eligible for another award. Applications shall consist of: (1) a supporting letter from the applicant's employer, manager or supervisor, attesting to the applicant's status as a full-time professional staff member; (2) a scientific abstract (original and 4 photocopies) for presentation, as described in the Registration Bulletin, accompanied by a completed Data Form (available on-line at http://resolution.umn.edu:591/MandM/DataEntry.html, or if inaccessible, by calling the Meeting Manager at 708/361-6045; electronic submission of the Data Form is encouraged); (3) a copy of the abstract to be sent by 15 February 1999 to the Chair of the Technologists' Forum: Ms. Beverly E. Maleeff, SmithKline Beecham Pharmaceuticals, Safety Assessment-US, UE0462, 709 Swedeland Road, King of Prussia, PA 19406. Phone: 610/270-7987; Fax: 610/270-7202; e-mail: Beverly_E_Maleeff-at-sbphrd.com. In order to be considered, completed applications must be received by 15 February 1999. Abstracts will be judged by the MSA Technologists' Forum. All applicants will be notified of the outcome in early March. Applicants not receiving the award will have the opportunity to withdraw their abstract if necessary.
Regards, Bev Maleeff Chair, MSA Technologists' Forum
I'd like to thank all those who responsed to my question. It is clear now that we should use double glid (Ni) instead glue to hold the sammple. Attached are messages I've received.
} From zrdai-at-u.washington.edu Mon Feb 1 16:06:29 1999
You might consider the following, (a thick, rather crude adhesive). It is: Sauereisen Insa-Lute adhesive, No. 1 paste. We used it to hold U-Si slices for polishing mechanically. The as mounted slices were then ion irradiated in a UHV vacuum chamber at 350 C approximately. The glue held pretty well, but can't be dissolved-unless the company has a special solvent. It outperformed several "high temp" glues that I tried on hotplate tests. Source: Sauereisen 160 Gamma Drive Pittsburgh Pennsylvania, 15238-2989
Phone: (412) 963-0303 FAX: (412) 963-7620
Bernard Kestel Materials Science Div. 9700 So. Cass Ave. Argonne, Ill., 60439
} From stephan-at-gecko.biol.wits.ac.za Mon Feb 1 16:06:29 1999
I was wondering if anyone in the microscopic community could recommend an outfit that can polish petrographic thin sections for EMP work. I need to can the samples processed within a month timeframe and I am willing to pay some for this. Many thanks
The Emory University Neurology Microscopy Laboratory, the University of Georgia Botany Department EM Laboratory, and Leica Microsystems, Inc.
Present a Cryo Techniques and Immunogold Workshop.
April 18-23, 1999 at the Campus of Emory University in Atlanta, GA.
Objectives
1. To provide researchers the opportunity to learn the theory and practice of cryo techniques for biological sample preparation and immunogold labeling.
2. To permit participants to process their own samples using these techniques under expert guidance.
I was requested to identify a crystal phase of small (5-7 micron) alumina particles embedded into copper. There are only a few particles, and all of them are on the surface. Any ideas how it can be done?
Thanks,
Alexander Titkov Millennium Inorganic Chemicals PO Box 245 Bunbury WA 6231 Australia Ph 61 8 9780 8505 W FAX 61 8 9780 8500 E-mail: atitkov-at-micl.com.au
Do any of you probers out there have a spare TAP crystal for a JEOL 8600/733 probe that they may be willing to sell? ..we've had something of an accident..
Thanks,
Stu -------------------------------------------- Stuart Kearns Electron Microbeam Laboratories Department of Earth Sciences University of Bristol Queens Road Bristol BS8 1RJ UK tel: (0)117 954 5435 fax: (0)117 925 3385 e-mail: Stuart.Kearns-at-bristol.ac.uk http://eugf.gly.bris.ac.uk --------------------------------------------
When I was an undergraduate research assistant at Wash U, I worked in the McDonnell Center for the Space Sciences. They were using an epoxy called E-7 which I have been using ever since. It comes in two parts (A & B) which you mix in a 2 to 1 ratio. Curing time is 2 hours at 150 degrees F. If cured right, there is really no outgassing or beam damage, even at higher micrprobe currents. Contact Pat Swan on the 4th floor, he might still use it. You can buy it from:
Techkits PO Box 105 Demerest, NJ 07627 201-768-7334
The latest price was $17.25/set for {10 sets. Hope this helps, Lou Ross Senior Electron Microscope Specialist Room 101 Department of Geological Sciences University of Missouri Columbia, MO 65211 (573) 882-4777 (573) 882=5458 fax www.missouri.edu/~geosclmr/ebaf.html
American Lab will be running an extensive review of new equipment being displayed at the upcoming PITTCON meeting (March 7-11, Orlando). For the first time, microscopy and related imaging will have its own section, as part of the on-going FOCUS ON MICROSCOPY column. If you are: (1) showing products which have not been exhibited or presented at a prior PITTCON and/or (2) introducing new products at this PITTCON please send copies of press kits, press releases, slides/product shots, and any other information to me at the address below. Indicate on the envelope that this is for the PITTCON Microscopy/Imaging review.
This article will need to go to press shortly after the meeting, so if I can get a head start on your materials, I would greatly appreciate it. Also, I will be on the show floor, following through on any materials received, so please enclose your booth number, name of a pertinent contact, and, if possible, a selection of times when they might be in your booth.
This article will appear in the May issue of American Lab.
Please call/email if you have any questions. Thanks in advance for your assistance.
Best regards, Barbara Foster Microscopy/Marketing & Education 125 Paridon Street, Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Contributing editor to American Lab ("Focus on Microscopy" column)
"Jeff" wrote: ============================================== Good Day,
Does anyone know of a dry etch that has a high selectivity to etch SiO2 preferentially to Si? We need to image these samples in a SEM. ================================================= This is usually done in a plasma etcher, using CF4 or some other reactive F gas of the more expensive types. You can get more information about this on our website given below. Typically, in a 100 watt barrel etcher, you can remove 1 um of SiO2 in about 30 minutes. The process is completely dry and is used in failure analysis laboratories.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
We are beginning to use the Historesin plus embedding medium supplied by Leica for immunocytochemistry of the retina by light microscopy. We will be using the images to count photoreceptor and retinal pigment epithelial cells of animals fed special diets to examine the effects of nutrition on the retina. I would like to communicate with others who have used this product to learn the best ways of storing material to preserve immunoreactivity for long periods of time and to share information on other technical issues.
You can respond to me directly at the address below.
Max Snodderly, Ph.D. Schepens Eye Research Institute 20 Staniford Street Boston, MA 02114, USA
In a message dated 2/1/99 9:03:47 PM Eastern Standard Time, kjtobin-at-uic.edu writes:
{ { I was wondering if anyone in the microscopic community could recommend an outfit that can polish petrographic thin sections for EMP work. I need to can the samples processed within a month timeframe and I am willing to pay some for this. } }
We recommend:
Mineral Optics Laboratory 29 "A" Street, P. O. Box 828 Wilder, Vermont 05088 USA 802-295-9373 802-295-7540 (FAX)
Yours truly, Steve Stokowski Stone Products Consultants Concrete Petrographers 10 Clark Street, Suite A Ashland, Massachusetts, 01721 USA 508-881-6364 http://members.aol.com/CrushStone/petro.htm
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Hello to all!
I am looking for a set of negative cassettes (30 plates), a cassette magazine box, and a cassette receiver box for Hitachi-600 TEM. Please let me know if any of you or your friends have a Hitachi TEM being taken down apart and wants to give away or trade these things.
Thanks in advance.
Gang Ning EM Facility Medical College of Wisconsin 414-456-8344
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Hello to all!
I am looking for a set of negative cassettes (30 plates), a cassette magazine box, and a cassette receiver box for Hitachi-600 TEM. Please let me know if any of you or your friends has a Hitachi TEM being taken down apart and wants to give away or trade these things.
Thanks in advance.
Gang Ning EM Facility Medical College of Wisconsin 414-456-8344
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Colleagues will be growing Vero cells (Green Monkey Kidney Cells) and intestinal epithelial cells on cover slips for subsequent ultrathin sectioning and TEM examination. I am interested to know which cover slips are best to use for this purpose. Can they be purchased sterile? I have checked the Ted Pella and EMS catalogs, they both sell non sterile cellulose acetate cover slips. Are there compatibility issues with resins and solvents? I am interested in any tips or tricks to smooth the way. Thanks, Sally Burns
Sally Burns Center for Electron Optics B5 Pesticide Research Center Michigan State University East Lansing, MI 48823 (517) 355-5004 Phone (517) 353-5598 FAX
I need some microneedles to dislodge some very fine particles (micron size) on my sample but still flexible enough to bend. Any information is appreciated.
As an electron microscopist who has come from a neurophysiology background, I have used various fine needles for "dusting" off debris. You need to find the one that feels right.
Cat whiskers are long, pointy, strong, and flexible. They are particularly good for chasing tiny bubbles out of microelectrodes or capillary tubes.
Finely drawn glass. Heat a pipet or rod over a Bunsen burner and draw it out until it breaks. Find somewhere along the long string that has the right size and flexibility, but won't break and leave more debris!
Cactus spines. They come in many shapes and sizes. Also useful for pinning down things for dissection that can't come into contact with metal.
Find someone in neurobiology who does microelectrode recording and get them to make some electrodes, which are capillary tubes drawn to a very fine point. You can probably get some in the micron range. Beveled, even!
Insect Minutin pins mounted on a stick are very strong, but may scratch your substrate. They can be ground down for a finer point.
Eyelashes, beard hair, and other body hairs each have different properties. Have fun experimenting.
Good luck!
Tina
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
We have used tungsten needles of about that dimension with our high- voltage electron microscope.. what we were looking for was a rather rigid "needle point" on which to mount micron sized objects. It was found that when we attempted to move these small specimens around and to get them to stick to the needles, the needles would bent very easily when they contacted the glass slides our specimens were prepared on. So you might try tungsten. We made them by electrolytically etching 5 mil. tungsten wires in NaNo3 solution, connecting the tungsten wire and a small graphite rod (1/8 inch dia.) to each of the terminals of a 6 volt AC transformer ( yes AC! ) and dipping both into the solution.The graphite rod was dipped well into the solution but the wtungsten would be dipped only an 1/8 inch or less below the surface. After a number minutes the wire would etch to a very small point, which could be examined under a light microscope until it was small enough for the application. Good luck.
For TEM of cultured cells, we grow the cultures on "Thermanox" tissue culture coverslips. From Nalge Nunc INternational, 50 sterile coverslips, 13 mm in diameter is catalog 174950. EMS also sells these thermanox cover slips in a variety of sizes (see page 143 of their cat XIII). The coverslips can be treated with all the same chemistry as tissue including propylene oxide and Spurrs epoxy, which are two components which solubilize polystyrene. These thermanox coverslips can be sunk cell side up in freshly made Spurrs, then following polymerization the coverslips can be removed by first sawing a small area of the epoxy/cell/substrate, then immersing in liquid nitrogen for a few seconds, then prying away the substrate. Now the embedded cells are immediate on the epoxy. Re-embed two fragments of the culture face to face for cross-sections, or cut the block parallel to the face for tangential sections. We particularly like the round 13 mm thermanox coverslips for immunocytochemistry of cultured cells since they can be floated cell side down in a drop of 100 microlitters antibody - gold conjugate, conserving reagents.
If you would like to grow a larger culture, you could also use "permanox" culture dishes, which are equally resistant to chemicals common in TEM processing. These are also available through EMS and other suppliers.
Good luck,
Doug ---------------------- Douglas R. Keene Associate Investigator Shriners Hospital Microscopy Unit 3101 S.W. Sam Jackson Park Road Portland, Oregon 97201 503-221-3434 DRK-at-shcc.org
Orlando in March!!!! To Register follow the local meetings in www.vacuum.org or http://www.msa.microscopy.com
Over 30 Invited Talks, 40 vendors, and over 40 student posters!
Golf Tourny on Sunday March 14 - www.pegasus.cc.ucf.edu/~ampac
AVS short courses and UCF/Vendor Sponsored Short Courses in Tripod Polisher and FIB TEM Specimen Preparation see www.pegasus.cc.ucf.edu/~ampac -----------------------------
Monday March 15, 1999
Opening and Keynote Address
8:00-8:15 am Welcome and Introduction, Lucille Giannuzzi, 1999 FL AVS/FSM Program Chair
8:15-9:00 am Keynote Address, Sam Durrance, Astronaut, Professor, University of Central Florida
Monday, March 15, 1999
Session I: Thin Films
Session Moderator: Maggie Puga-Lambers, University of Florida
9:00-9:25 am Tim Anderson, Chemical Eng. Dept., University of Florida
9:25-9:50 am CONCENTRATION DEPTH PROFILING OF IMPURITIES AND DOPANTS IN FLAT PANEL DISPLAYS AND GLASSES BY SIMS, Temel Buyuklimanli, Evans East
9:50-10:15 am MODIFICATION AND CHARACTERIZATION OF DEFECT STATES IN ZnO FILMS, Gregory J. Exarhos and Charles F. Windisch, Jr., Pacific Northwest National Laboratory
10:15-10:45 am Coffee Break
10:45-11:10 am FUNDAMENTALS OF TUNGSTEN CMP DURING CMOS DEVICE FABRICATION, Rajiv Singh, Engineering Research Center for Particle Science and Technology, University of Florida
11:10-11:35 am SURFACE ANALYSIS APPLICATIONS IN SEMICONDUCTOR TECHNOLOGY, Bridget Rogers, Vanderbilt University
11:35-12:00 pm THE INFLUENCE OF AIR ON THE PROPERTIES OF THIN FILMS DEPOSITED FROM BEAMS OF NANOPARTICLES USING A COPPER SOURCE, F. K. Urban, III, A. Khabari, A. Housseini-Tehrani, P. Griffiths, and G. Fernandez
12:00-1:00 pm Lunch Monday, March 15, 1999
Session II: Microscopy of Biological Samples
Session Moderator: Karl Muffly, University of South Florida Jo Ann Moore, University of South Florida
9:00-9:25 am DIGITAL MANIPULATION OF ACQUIRED IMAGES, WHAT IS POSSIBLE VS. WHAT IS ETHICAL, John Kinnamon, University of Denver and The Rocky Mountain Taste and Smell Center
9:25-9:50 am THE USE OF CORRELATIVE MICROSCOPY IN BIOLOGICAL PROBLEM SOLVING, Ralph Albrecht, University of Wisconsin
9:50-10:15 am APPLICATIONS OF A 300 KV, FIELD EMISSION ELECTRON MICROSCOPE IN STRUCTURAL BIOLOGY, Kenneth A. Taylor, Florida State University Institute for Molecular Biophysics
10:15-10:45 am Coffee Break
10:45-11:10 am DECONVOLUTION VS STANDARD FLUORESCENCE MICROSCOPY, Karl Muffly, University of South Florida College of Medicine
11:10-11:35 am SURFACE AND MICROSCOPIC ANALYSIS OF BIOMATERIALS AS THEY CHANGE IN VIVO: HOW FAR ARE WE FROM NEEDED DATA?, Chris Batich and Anthony Brennan, University of Florida
11:35-12:00 pm CARBOHYDRATE DEPOSITION PATTERNS IN ETIOLATED SOYBEAN SEEDLINGS GROWN IN MICROGRAVITY, E.C. Stryjewski, NASA Gravitational Biology Laboratory, Dynamac Corp., K.M. Johnson, National Research Council, NASA/KSC, W.C. Piastuch1, H.G. Levine1, and L.H. Levine, NASA Gravitational Biology Laboratory, Dynamac Corp., O. Martynenko3, and V. Prima,, Institute for Molecular Biology and Genetics, National Academy Of Sciences, Ukraine
12:00-1:00 pm Lunch
1:30-3:30 pm WORKSHOP ON MANIPULATING DIGITAL IMAGES, John Kinnamon, University of Denver and The Rocky Mountain Taste and Smell Center
3:30-4:00 pm Florida Society for Microscopy Annual Business Meeting
3:30-6:00 pm Competition Session and Student Competition (Session IV) Monday, March 15, 1999
Session III: Surface Science and Analysis
Session Moderators: Sudipta Seal, University of Central Florida
1:00-1:25 pm WETTABILITY AND INTERFACES IN METAL/NITRIDE SYSTEMS, Natalia Sobczak, Foundry Research Institute, Cracow, POLAND
1:25-1:50 pm SOFT X-RAY FLUORESCENCE SPECTROSCOPY IN MATERIALS SCIENCE, E. Joseph Nordgren, Uppsala University, Uppsala, Sweden
1:50-2:15 pm MAGNETIC PHASE DIAGRAMS OF ULTRA-THIN FILM BINARY ALLOYS FOR SPIN-VALVE APPLICATIONS, Brian Tonner, University of Central Florida
2:15-2:40 pm PRACTICAL ASPECTS OF HIGH RESOLUTION XPS WITH MONOCHROMATIC AlKA X-RAYS, A.C. Miller, Lehigh University
2:40-3:05 pm STUDIES OF OXIDATION AND CORROSION USING AN ANAEROBIC CELL APPROACH, Peter M.A. Sherwood, Kansas State University
3:05-3:30 pm MICRO-ESCA/NEXAFS AT A THIRD GENERATION SYNCHROTRON LIGHT SOURCE, J. H. Underwood, U. Kleineberg, S. Mrowka, P. J. Batson, S. B. Rekawa, M. S. Jones, R. C. C. Perera, P. N. Ross, University of California, Berkeley
3:30-4:00 pm Coffee Break
3:30-6:00 pm Competition Session and Student Competition (Session IV)
Monday, March 15, 1999
Session IV: Poster Session
Session Moderators: Larry Plew, Cirent Semiconductor
CHARACTERIZATION AND SURFACE ANALYSIS OF HYDROXOCARBONATE COMPOUNDS, B. B. Adhyaru, K. R. Williams, and V.Y. Young, University of Florida
INTERFACIAL REACTIONS BETWEEN METAL/P-GaN FOR FORMATION OF OHMIC CONTACTS, M. Ahonen, B. Liu, P.H. Holloway, University of Florida
SURFACE METASTABLE STRUCTURE OF KTa03 (001) BY HELIUM ATOM SCATTERING, E. A. Akhadov, T. W. Trelenberg, J. A. Li, J. G. Skofronick, S. A. Safron, Florida State University and L. A. Boatner, Oak Ridge National Laboratory
SIMS STUDY OF DIFFUSION PHENOMENA OF METAL ELEMENTS IMPLANTED INTO SILICON, Elvira V. Anoshkina,a,b) Hughes Francois-Saint-Cyr,a,b,c) Ashfaq Hussain,a,b) , Isaiah Oladeji,d) Fred A. Stevie,e) Lee Chow,b,d) Dan Zhou a-dUniversity of Central Florida, eCirent Semiconductor
FAILURE ANALYSIS : AN AES-SEM STUDY, K. R. Beaulieu, (UNDERGRADUATE) A. S. Kale, S. Seal, V. Desai, University of Central Florida
IDENTIFICATION OF SURFACE CHEMICAL FUNCTIONAL GROUPS IN POLYMER MEMBRANES: AN X-RAY PHOTOELECTRON SPECTROSCOPY STUDY, Sharon D. Beverly 1,2, Satyajit V. Shukla, 3,4 Seungkwan Hong, 2 and Sudipta Seal3, 4, 1NASA, 2-4University of Central Florida
STUDY OF CORROSION FAILURES UNDER ATMOSPHERIC CONDITIONS, L.A. Bracho, V. H. Desai, S. Seal, University of Central Florida
ANISOTROPIC PATTERN TRANSFER IN GaN BY PHOTO-ENHANCED WET ETCHING, Hyun Cho(1), S.M. Donovan(1), C.R. Abernathy(1), J. Han(2), R.J. Shul(2), F. Ren(3) and S.J. Pearton(1), (1) & (3) University of Florida (2) Sandia National Laboratories
NOVEL EMITTER BASE SELF-ALIGNED PROCESS FOR AlGaN/GaN HETEROJUCTION BIPOLAR TRANSISTORS, X. A. Cao1, H. Cho1, S. J. Pearton1, C. R. Abernathy1, F. Ren1, J. Han2, R. J. Shul2, and A. G. Baca2, 1 University of Florida, 2 Sandia National Laboratories
HELIUM ISOLATION IMPLANT FOR GALLIUM NITRIDE BASED FIELD EFFECT TRANSISTORS, G. Dang1, X. Cao1, F. Ren1, S.J. Pearton1, J. Hang2, and R. J. Shul2, 1University of Florida, 2Sandia National Labs
CAPILLARIZATION OF SKELETAL MUSCLE IN RATS UNDERGOING HEART FAILURE, Hans Degens, Rebecca K. Anderson, Karl E. Muffly and Stephen E. Always, University of South Florida
UN-ANNEALED AND ANNEALED PD ULTRA-THIN FILM ON SIC CHARACTERIZED BY ATOMIC FORCE MICROSCOPY, SCANNING TUNNELING MICROSCOPY, AND X-RAY PHOTOELECTRON SPECTROSCOPY, K. Elshot, Mechanical Materials and Aerospace Engineering, University of Central Florida, Orlando, FL 32826, W. Lu, D.T. Shi,E. Bryant, K. Lafate, H. Chen, A. Burger, W. E. Collins, Department of Physics, Fisk University, Nashville, TN 37208
FUNDAMENTAL PROPERTIES ON E-BEAM EVAPORATED ZnS:Mn AND Zn1-xMgxS:Mn ELECTROLUMINESCENT THIN FILMS, Tao Feng, Mark Davidson, Paul Holloway, University of Florida
DESIGN, DEVELOPMENT AND TESTING OF A COMPUTERIZED DATA ACQUISITION AND CONTROL SYSTEM FOR A NANOPARTICLE DEPOSITION SYSTEM, Frank K. Urban and G Fernandez, Florida International University, Miami, Florida
CHARACTERIZATION OF THE DIFFUSION PROPERTIES OF Mg, Cl, K, Ge, AND Mo IN SILICON BY SIMS, Hughes Francois-Saint-Cyr,a,b,c) Elvira V. Anoshkina,a,b) Ashfaq Hussain,a,b) ,Fred A. Stevie,e) Lee Chow,c,d) Kathleen Richardson, a,b,c) and Dan Zhou a,b), a-dUniversity of Central Florida, eCirent Semiconductor
A STUDY OF THE SURFACE COMPOSITION OF KTAO3 DOPED WITH CA, BA, SR, AND NB, P.W. Gresser Florida State University
NOZZLE DESIGN IN A DIFFERENTIALLY PUMPED NANO-PARTICLE INSTRUMENT, Peter D. Griffiths and Frank K. Urban, Florida International University
INTERFACIAL CHRACTERISTICS OF AlN TO Si, SiC AND GaN, K. Harris, B. P. Gila, F. Ren, J. Deroaches, K. N. Lee, J. D. MacKenzie, C. M. Zitterling+, M. Ostling+, S. N. G. Chu**, C. R. Abernathy, and S. J. Pearton, University of Florida, Gainesville, FL, +KTH, Royal Institute of Technology, Stockholm, **Bell Laboratories, Lucent Technologies
SELECTIVE DRY ETCHING USING INDUCTIVELY COUPLED PLASMAS: GaAs/AlGaAs AND GaAs/InGaP, D.C. Hays, H. Cho, K.B. Jung, Y.B. Hahn*, C.R. Abernathy, S.J. Pearton, and F. Ren, University of Florida, W.S. Hobson, Bell Laboratories, Lucent Technologies
HOT FILAMENT CVD OF DIAMOND THIN FILMS, Ashfaq Hussain, Lee Chow, and Dan Zhou, University of Central Florida
QUANTITATIVE COMPARISON OF VON WILLBRAND FACTOR (VWF) EXPRESSION IN HUMAN NON-MALIGNANT AND MALIGNANT TISSUE USING CONFOCAL LASER SCANNING MICROSCOPY (Undergraduate), D Janarious, J Biggerstaff, JL Francis, Walt Disney Memorial Cancer Institute
DIETARY MODIFICATION AND THE ALZHEIMER'S-LIKE PHENOTYPE IN mAPP/mPS1 TRANSGENIC MICE, P.T. Jantzen, M.N. Gordon and D.G. Morgan, University of South Florida
COMPARISON OF CHARACTERISTICS DRY ETCHING OF LaCaMnO3, NiMnSb AND NiFe THIN FILMS, K. B .Jung(1), Hyun Cho(1), J. J. Wang(1), J. Caballero(1), Tao Feng(1), J. R. Childress(2), K. H. Dahmen(3) and S. J. Pearton(1), (1)University of Florida, (2)IBM Almaden Research Center (3)Florida State University
FABRICATION OF DC-MAGNETRON SPUTTERED 70Ti-30Al THIN FILMS, A. S. Kale, K. R. Beaulieu, K. B. Sundaram, V. H. Desai, S. Seal, University of Central Florida
THE ATOMIC FORCE MICROSCOPY INVETIGATION OF THIN FILM DEPOSITED FROM NANOPARTICLE SOURSE, F.K. Urban, A. Khabari, Florida International University
COMPARISON OF ZnS:TbOF THIN FILMS DEPOSITED BY R.F MAGNETRON SPUTTERING USING ZnS, TbOF MIXED TARGET AND SEPERATED ZnS,TbOF TARGETS, Jongpyo Kim, Mark Davidson, Barbara Speck, David Moorehead, Qing Zhai, and Paul Holloway, University of Florida
ELECTRON BEAM DEGRADATION OF OXIDE AND SULFIDE THIN FILM PHOSPHORS FOR FIELD EMMISION DISPLAYS, Caroline A. Kondoleon, Billie Abrams, Philip Rack* and Paul Holloway, University of Florida, Rochester Institute of Technology
ELECTRON CYCLOTRON RESONANCE CHEMICAL VAPOR DEPOSITED SILICON NITRIDE FOR T-GATE PASSIVATION, J. LaRoche1, F. Ren1, S.J. Pearton1, J. R. Lothian2, J.W. Lee3, and D. Johnson3, 1University of Florida, 2Multiplex Inc, 3Plasma-Therm, Incorporated
DRY ETCHING OF BaSrTiO3 AND LaNiO3 THIN FILMS IN INDUCTIVELY COUPLED PLASMAS, K. P. Lee, K. B. Jung, A.Srivastava, D. Kumar, R. K. Singh and S. J. Pearton, University of Florida
EFFECTS OF Ni THICKNESS ON Ni/Ti/Ag OHMIC CONTACT TO p-GaN, B. Liu, M. H. Ahonen and P. H. Holloway, University of Florida
TITANIUM MAGNESIUM NICKEL ALLOY AND HYDROGEN STORAGE, Janice K. Lomness, Sudipta Seal, Michael D. Hampton, and Meredith Stowell (Undergraduate), University of Central Florida
(Undergradutate) CHARACTERIZATION OF BEAM PRODUCED BY PULSED ARC CLUSTER ION SOURCE, Samantha A. Moore and Anne J. Cox, Eckerd College
COMPARISON OF THE MICROSTRUCTURE AND ELECTROLUMINESCENT PROPERTIES OF ZnS:Mn DEPOSITED BY SPUTTERING AND ATOMIC LAYER EPITAXY, David J. Moorehead Karen E. Waldrip, M.R. Davidson*, J.H. Lee, B. Pathangey*, M.Puga-Lambers*, K.S. Jones, P.H. Holloway, University of Florida, and S.S. Sun and C.N. King, Planar Systems
TRI-LAYER ELECTRON BEAM RESIST FOR SUBMICRON T-GATE GaN BASED FIELD EFFECT TRANSISTORS, M. Mshewa, H. Hudspeth, F. Sharifi, S. J. Pearton, and F. Ren, University of Florida
A MODIFICATION OF THE VARIABLY DAMPED LEAST SQUARE ALGORITHM ASSISTED BY MEASUERED DATA POINTS AND DERIVATIVE PRESELECTION FOR IMPROVEMENT OF SOLUTIONS, J. Pontillo, F.K. Urban, Florida International University
PULSED CURRENT ELECTROMIGRATION, Cross Reardon and Rolf Hummel, University of Florida
ILLUSTRATION OF CAPILLARY PERFUSION IN HYPERTROPHIED CARDIAC MUSCLE USING THE FLUORESCENT DYE, THIOFLAVIN-S, Corey A. Schoder, Hans Degens, Don R. Hilbelink, Karl E. Muffly University of South Florida
FORMATION OF SILVER SULFIDE NANO-PARTICLES BY NOVEL SOL-GEL METHOD FOR INDUSTRIAL APPLICATIONS, S. Shukla and S. Seal, University of Central Florida
CO-LOCALIZATION OF GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP), OX-42 AND OX-6 IN SPINAL CORD FOLLOWING SCIATIC NERVE DAMAGE, Stacy Sinibaldi, Edward Haller, and Samuel Saporta University of South Florida
CHARACTERIZATION OF THE CONSORTIUM BETWEEN THE RED TIDE CAUSING DINOFLAGELLATE GYMNODINIUM BREVE, AN UNIDENTIFIED BACTERIUM AND A PHAGE, Theresa R. Slifko, Lee Houchin, Anthony Greco, and John H. Paul, University of South Florida
INVESTIGATIONS INTO CHEMICAL MECHANICAL POLISHING OF TUNGSTEN USING VARIOUS ELECTROCHEMICAL AND SURFACE SCIENCE TECHNIQUES, Dnyanesh Tamboli, Sudipta Seal, Vimal Desai University of Central Florida
THE MINERAL CONTENT AND CELLULAR STRUCTURE OF HETEROTOPIC BONE, Gregory S. Taylor University of Florida College of Medicine, and Melinda K. Harman and W. Andrew Hodge, Orthopaedic Research Laboratory
EFFECTS OF FIB OPERATING CONDITIONS ON BEAM DAMAGE OCCURRING IN SILICON TEM SAMPLES, C.A. Urbanik, B.I. Prenitzer, L.A. Giannuzzi, S.R. Brown1, R.B. Irwin1, B. Rossi2 , T.L. Shofner2, and F.A. Stevie1, University of Central Florida, 1Cirent Semiconductor, 2The Bartech Group
IMPROVED PERFORMANCE IN THIN FILM ELECTROLUMINESCENT PHOSPHORS BY DONOR DOPING, K.E. Waldrip, J.S. Lewis, III, Q. Zhai, D. Moorehead, M.R. Davidson*, and P.H. Holloway, University of Florida, and S.S. Sun, Planar Systems, Inc.
EFFECTS OF FLUX AGENTS ON THE MICROSTRUCTURE AND ELECTROLUMINESCENCE OF SPUTTERED ZnS:Mn THIN FILM PHOSPHORS, Qing Zhai, Karen Waldrip, Jay Lewis, Jungpyo Kim, David Moorhead, Jinghong Li, Kevin Jones, and Paul Holloway, Maggie Puga-Lambers, Barbara Speck, and Mark Davidson, Microfebritech, University of Florida
ICP Cl/Ar PLASMA DAMAGE ON GaN SCHOTTKY, A. Zhang1, H. Cho, F. Ren1, S.J. Pearton1, J. M. Van Hove2, P. P. Chow2, R. Hickmand2, J. J. Klaasen2 and R. J. Shul3, 1University of Florida, 2SVT Associates, 3Sandia National Labs
MICROSTRUCTURE EVALUATION OF STRESS CORROSION CRACKING USING FIB TECHNIQUES, Hanlin Zhang, Brian Kempshall, Carrie Urbanik and Lucille A. Giannuzzi, University of Central Florida
Tuesday, March 16, 1999
Session V: Microscopy of Physical Samples
Session Moderator: Lucille A. Giannuzzi, University of Central Florida
8:30-8:55 am ANALYTICAL MICROSCOPY IN THE REAL SEMICONDUCTOR PROCESSING WORLD, Ron Anderson, IBM Analytical Services
8:55-9:20 am FIB APPLICATIONS IN MATERIAL SCIENCE PROBLEMS, M.W. Phaneuf, J. Li, G.A. Botton*, L. Weaver, Fibics Incorporated, *Materials Technology Laboratory
9:20-9:45 am FOCUSED ION BEAM IMAGING OF MICROELECTRONIC STRUCTURES, Ann N. Campbell, Sandia National Laboratories
9:45-10:10 am DETERMINATION OF THE COMPOSITION OF GRAIN BOUNDARIES AND INTERFACES BY X-RAY MICROANALYSIS, David B. Williams, Lehigh University
10:10-10:40 am Coffee Break
10:40-11:05 am MATERIALS APPLICATIONS OF ELECTRON HOLOGRAPHY, Altaf Carim, The Pennsylvania State Univeristy
11:05-11:30 am PHASE MAPPING AND PHASE IDENTIFICATION USING ELECTRON BACKSCATTER DIFFRACTION, David J Dingley and Stuart I Wright, TexSEM Laboratories
11:30-11:55 pm AFM, Phil Russell, North Carolina State University
12:00-1:00 pm Lunch and Vendor Talks (Session VI)
Tuesday, March 16, 1999
Session VI: Vendor Presentations
Session Moderators: Fred Stevie, Cirent Semiconductor
12:10-12:20 pm LOW ENERGY ION MILLING FOR TEM, David Henriks, South Bay Technologies
12:20-12:30 pm INNOVATIONS IN MASS FLOW CONTROLLERS FROM UNIT, Greg Vaughan, Schoonover, Inc.
12:30-12:40 pm RECENT DEVELOPMENTS IN ATOMIC FORCE MICROSCOPY, Matt Thompson, Digital Instruments
12:40-12:50 pm NEW PRODUCTS FROM SPECS: Er-LEED, ULTRA HIGH RESOLUTION EEES: DELTA0.5, AND PLASMA UV SOURCE UV300, Dietrich von Diemar, SPECS U.S.A.
Tuesday, March 16, 1999
Session VII: Electronic Materials
Session Moderator: Drew Hoff, University of South Florida
1:00-1:25 pm LOW FIELD ELECTRON EMISSION FROM UNDOPED NANO-STRUCTURED DIAMOND, W. Zhu, G. P. Kochanski and S. Jin, Bell Laboratories, Lucent Technologies
1:25-1:50 pm SYNTHESIS AND APPLICATIONS OF NANOCRYSTALLINE DIAMOND FILMS, Dieter M. Gruen, Argonne National Laboratory
1:50-2:15 pm CHARACTERIZATION OF SHALLOW JUNCTIONS USING SECONDARY ION MASS SPECTROMETRY, Charles W. Magee, I.M. Abdelrehim, T.H. Buyuklimanli, J.T. Marino and W. Ou, Evans East
2:15-2:40 pm NOVEL PROCESSING OF ELECTRONIC MATERIALS, W. V. Lampert, Air Force Research Laboratory, WPAFB, OH
2:40-3:05 pm GaN BASED ELECTRONICS FOR HIGH TMEPERATURE APPLICATION, F. Ren1, S. J. Pearton1, C. R. Abernathy1, J. M. Van Hove2, P. P. Chow2, R. Hickmand2, J. J. Klaasen2, J. Han3, A. G. Baca3, and R. J. Shul3, 1University of Florida, 2SVT Associates, 3Sandia National Labs
3:05-3:30 pm LOW ENERGY IMPLANTATION AND SHALLOW JUNCTIONS IN Si, Kevin Jones, University of Florida
3:30-4:00 pm Coffee Break, Student Awards and Door Prizes
Tripod Polisher, instructor: Ron Anderson March 12-13 FIB, instructors: Lucille Giannuzzi and Fred Stevie March 18-19
in conjunction with the FL AVS/FSM meeting week of March
for information see www.pegasus.cc.ucf.edu/~ampac or contact lag-at-mail.ucf.edu
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
Tong - You might find that a glass needle will work for your purpose and you can easily make them yourself to almost any desired thinness. Simply take a thin 8 or 10 inch long glass rod (can be hollow too) and heat near the center with a bunsen burner or other source. After glowing pull the ends apart and you will draw the glass down to a suitable thickness. At the micron level glass is relatively strong for moving particles and as you will find quite flexible.
Dave Joswiak Dept. of Astronomy Univ. of Washington Seattle, WA 98195 (206)543-7702
On Tue, 2 Feb 1999, Tong Wang wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, } } I need some microneedles to dislodge some very fine particles (micron size) } on my sample but still flexible enough to bend. } Any information is appreciated. } } Tong } } } } }
Postdoctoral Positions in Nuclear Materials Science - PD994571
The Materials Science and Processing Group (NMT-11) at Los Alamos National Laboratory is seeking qualified applicants to fill two postdoctoral positions in the science of nuclear materials. Successful candidates will work with technical staff members in the Materials Science and Technology (MST) and Nuclear Materials and Technology (NMT) Divisions. Both positions require the willingness to work on radioactive materials and the ability to obtain a DOE Q clearance (which usually requires US citizenship).
Position One: Electron Microscopy of Plutonium and Ceramic Actinide Waste Forms. The individual will have access to (a) the Electron Microscope Facility in MST for microstructural studies of unirradiated and surrogate materials and (b) the Materials Characterization Facility in NMT for analysis of radioactive materials. Techniques available for this research include SEM, OIM, TEM, HRTEM and STEM. This work will support research efforts in radiation effects in ceramics and plutonium characterization for weapons programs. Candidates must have prior experience in electron microscopy and microanalysis. Contact Jeremy Mitchell (505-665-3934, jeremy-at-lanl.gov) or Kurt Sickafus (505-665-3457 kurt-at-lanl.gov) for further technical information.
Position Two: Radiation Damage in Metals, Self-radiation Damage in Plutonium Metal, Alloys and Compounds. This work is basic research in support of plutonium metallurgy and chemistry for the weapons programs. Candidates must have prior experience in x-ray and/or neutron diffraction and the associated data analysis with GSAS, knowledge of mechanisms of radiation damage in metals and some knowledge of condensed matter physics. The individual will also have access to calorimeters, SEM, OIM, and TEM. Contact Luis Morales (505-665-7703 lmorales-at-lanl.gov) for additional technical information.
A Ph.D. in Materials Science, Metallurgical Engineering, Geosciences, or a related field completed within the last three years or soon to be completed is required. Candidates may compete for a Director's Fellowship and outstanding candidates may be considered for the prestigious J. Robert Oppenheimer, Richard P. Feynman or Frederick Reines Fellowships. Further details about the Postdoctoral Program may be found at: http://www.hr.lanl.gov/postdoc/ For consideration, submit a resume and publications list along with a cover letter outlining current research interests to postdoc-jobs-at-lanl.gov (no attachments, please!)
OR SUBMIT TWO COPIES to:
Postdoc Program Office, PD994571 MS P290 Los Alamos National Laboratory Los Alamos, NM 87545
NOTE: Advertisement #PD994571 must be referenced in the e-mail Subject line (or the address) and cover letter.
Affirmative Action/Equal Opportunity Employer. Individuals with disabilities needing reasonable accommodation should call (505) 667-8622. A Teletype Device for the Deaf (TDD) is available by calling (505) 665-5357. Los Alamos National Laboratory is operated by the University of California for the US Department of Energy. ============================ Jeremy N. Mitchell MS G730, NMT-11 Los Alamos National Laboratory Los Alamos, NM 87545 Phone: 505-665-3934 Fax: 505-665-4013
Dear Sally, The coverslips that everyone uses is my Thermanox plastic coverslps which are sterile and come in many different sizes depending on your need. They may be found wither in our hard copy catalog or on our website at www.emsdiasum.com. Go to our online catalog and click chapter 7. They are listed under Thermanox. In our hard copy catalog XIII they may be found on page 143. Please let me know if I may be of further assistance to you.
Sincerely, Electron Microscopy Sciences 215-646-1566
The best way I know of preparing pigments for TEM analysis is by blowing an aerosol of the sample dispersed in alcohol/water solution over a hot plate and collecting the sample on a grid placed at the far end of the hot plate. The company I work for is concerned about the safety issues surrounding this so does anybody know of any less hazardous sample preps. or a simple containment facility for the aerosol spray? I thought about building a glass box for containment but are there any commercially available containment/sample prep. units?
Thanks
Brian Manzor e-mail: brian.manzor-at-grmouth.zeneca.com
The best way I know of preparing pigments for TEM analysis is by blowing an aerosol of the sample dispersed in alcohol/water solution over a hot plate and collecting the sample on a grid placed at the far end of the hot plate. The company I work for is concerned about the safety issues surrounding this so does anybody know of any less hazardous sample preps. or a simple containment facility for the aerosol spray? I thought about building a glass box for containment but are there any commercially available containment/sample prep. units?
Thanks
Brian Manzor e-mail: brian.manzor-at-grmouth.zeneca.com
Hi Michael! To avoid tissue curling try 50% or 30% ETOH in the first dehydrating step. You can do dyhradation in cell-culture multiwells. You=B4ll have to remove the specimens if you=B4d like to use Propylene ox= ide because this interacts with the multi-well plastic. If you like to embed the sections flat, do it on silicated glass slides(o= r try a slide, coverd with slightly fatted aluminium foil) , put a drop of Epon/araldite on it, leave it at 45=B0C in the oven (12-24h) and then put= BEEM Capsules filled with Epon/araldit on the top(take care of bubbles!) , polymerize 2 days at 60=B0C. If you can=B4t remove the glass slide easily= , try it with fluid nitrogen. Good luck, Michael
MICHAEL DELANNOY schrieb:
} -----------------------------------------------------------------------= - } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Co= m } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.htm= l } -----------------------------------------------------------------------. } } Greetings, } I need assistance with flat embedding of rat cerebellum } (IEM-DAB), that is 100 um thick and 1.5 x 0.5 cm dimensions. Basically } we are worried about tissue curling during the dehydration. My first } inclination is argarose embed (before epon), but I would take any exper= t } advise. } Thank you, } } Mike D
Sprays can be contained to some extent within a box for spraying thin-layer chromatographic plates, 20 x 20 cm or a little larger. This has to be placed in a hood, since it won't catch and retain the smallest droplets. They come in cardboard and plastic versions. I can't find one in VWR, but any big Web catalog with a search capability should lead you to one.
Leonard Corwin Research Chemist Fort Dodge Animal Health Princeton, NJ 08543-0400
Hi all. We have identified a need for some hands on WDS training here in South = Africa. A number of our clients have either changed operators or = upgraded to new integrated systems. So we would like to know if there is = any one who would be able to help with presenting a WDS course here in = South Africa. No need to panic, South Africans do have money and are = willing to pay, not too much mind you.=20
We are open to the course content, however would like to cover a bit of = customer care operations, calibration and set up and then theory and = hands on operation.=20 We will have a Jeol 733 with an Advanced Microbeam / Edax integrated = system available by about June onwards.=20
If any one is interested please let us know so we can discuss details. Thanks
Luc Harmsen=09 Anaspec, South Africa International technical support on microscopy. Tel: +27 (0) 11 476 3455 Fax:+27 (0) 11 476 7290 anaspec-at-icon.co.za
Hello All, Thanks to all those who offered suggestions for the damping of vibrations sometimes experienced when using a light microscope. Once again you have proven to be an ingenious lot! Users have their microscope sitting on platforms supported by any of the following; tennis balls, "sandwiches" of bubble pack and aluminum plates or inner tubes all of which indicates that the pneumatic tables or benchtop isolation systems are preferable to the marble tables. An alternative approach is to support the microscope on a platform beneath which are sandwiched layers of Sorbothane (elastomer) and aluminum plates. One simple suggestion was to "plant" each leg of a conventional table; supporting your microscope, into its own little box of sand. I'm a little wary of this last suggestion as some of the laboratory wildlife might find the sand an attractive litter box!
Regards, Paul
Paul J. Gerroir Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
To Dr. Naseem: TEM results on paraffin processed tissue will only be as good as the processing procedure used by the histology lab. In my experience, most histology labs use a very aggressive protocol, which causes extraction of cellular components to the point where only nuclei and possibly cell membranes might be recognizable. Laboratories that handle large volumes and are concerned with quick turnaround generally dehydrate and clear the heck out of specimens so they don't have to go back and reprocess if something was too big, not well fixed, etc. Our laboratory provides good fixation, gentle dehydration, clearing and infiltration of routine histology specimens so that when I have to do EM on one of these, there is usually enough cellular matrix left to make the effort worthwhile. I have been able to resolve Birbeck bodies, tonofilaments, junctions, microvilli and immune complex deposits (in glomeruli) in paraffin processed specimens. Good luck! Bob Santoianni Emory University Hospital Atlanta, Georgia robert_santoianni-at-emory.org
To process cell cultures for grown on round, 13 mm Thermanox tissue culture cover slips for TEM, we use the following hardware:
For fixation, we use porcelain multi-well dishes. These are what most people refer as "staining dishes". They are white, measure about 3.5 x 4.5" and have 12 depressions. We use these for glutaraldehyde, buffer rinses and OsO4. OsO4 can be completely rinsed from the dishes. Once in the last buffer after OsO4 and prior to ethanols and propylene oxide, we transfer the disks to 50 ml polypropylene culture tubes, such as Fisher 05-539-7 (these are sterile, but certainly it is not necessary to pay for sterile containers). There is plenty of room to enter a pipette for fluid exchange without touching the culture disks, and the culture surface will not touch the walls of the cylindrical tube so there is no worry that the cells will be rubbed off. Given the depth of the tube, we do not worry too much that the culture will dry out as fluids are exchanged, since the atmosphere within the tubes is fairly saturated with solvent vapor. Still, fluid exchange is done quickly. The polypropylene tubes are resistant to P.O. and Spurrs. Do not use tubes made from polystyrene as they will dissolve. Once infiltrated with the last change of epoxy, we fill a resin-resistant container with epoxy to a minimum depth of 5 mm, sink the disk so that the cells face up, then polymerize the epoxy. We steal the polypropylene lids from wheaten snap-cap vials (Fisher #0333520E) which are of the appropriate diameter for embedding 13 mm coverslips. Wearing a dust mask, We use a jewelers saw to free small blocks of embedded culture, loosen the cover slip with liquid nitrogen, then remove the disk which exposes the culture to the surface of the block. We then either re-embedded (in some of the same batch of media which was used to infiltrate the culture) face to face for cross sections, or cut the blocks parallel to the culture surface for tangential sections.
Good luck,
Doug Keene EM Facility Shriners Hospital for Children DRK-at-shcc.org
} } Hello All, } Does anyone out there have any suggestions for getting good SEM } cross sectional images from dried wood? I have a student who would } like to image the microstructure of several types of wood. All the
} } Thanks, } Scott
Hi Scott,
I think the classic paper you want is Exley et al. (1973 ?) J. Microscopy, vol 101, 21-30 "Preparation of wood specimens for the scanning microscope".
I don't have a copy handy to check, but I think this paper emanated from good ol' New Zealand! As I recall, the authors were able to cut fresh lignified wood satisfactorily using a fresh razor blade for each cut, and then cleaned the surfaces with hypochlorite solution before drying and coating. Most specimens were OK with just air drying but delicate features needed CPD. The authors often cut the one specimen in two planes to good effect, e.g. transverse and radial longitudinal.
If you want to look at long-dead wood it might be too hard to cut cleanly. You might need to soak it in something first. Exley et al. might have suggestions.
Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department Faculty of Medicine & Health Sciences University of Auckland Private Bag 92019 Auckland New Zealand
-----Original Message----- } From: Gerroir, Paul J {Paul.Gerroir-at-crt.xerox.com}
} One simple suggestion was to "plant" each leg of a } conventional table; supporting your microscope, into its own little box of } sand. I'm a little wary of this last suggestion as some of the laboratory } wildlife might find the sand an attractive litter box!
Mix red pepper with the sand that will keep the kitty out of the sand.
Gordon
Gordon Couger gcouger-at-couger.com Owner PRAG-L PRactical AGriculture List www.couger.com/prag-l Stillwater, OK 405 624-2855 GMT -6:00
I posted a notice several months back about a Siemens 101 Transmission Electron Microscope that was up for grabs. Although we had some interest, no one decided to take it. At this point, we have a major space crunch and need to dismantle and get rid of it. I've been told it contains 25 gms of liquid mercury which should be removed before disposal. Can anyone refer me to a technician familiar with this equiment in the NY area who could assist us in this project. Thanks in advance for your help. Please reply directly to me. I am also posting this notice on the safety list. ---------------------------------------- Gerry Griffin Environmental Services NYU Medical Center Email: Gerry.Griffin-at-med.nyu.edu
I'm studying the transport and localization of some proteins in the cytoplasm of cultured cells. The cells are fixed in paraformaldehyde and stained with a avidin-biotin-fluorescein system. I need to do double staining for this protein and the Golgi, so I intend to use the Bodipy-tr-ceramide probe, as suggested by Molecular Probes .
I would like to know if someone has experience or worked with this specific probe. Particularly I need to know if this probe may be used with fixed cells, that is, after the cells are fixed.
I will be very grateful for any information
Thanks in advance
Francisco Javier Hernandez-Blazquez IARC-WHO Unit of Multistage Carcinogenesis 150 Cours Albert Thomas Lyon - France
We are interested in buying a variety of used AFM and STM equipment. To see our wish list, go to our web page (or contact us directly) http://a1.com/asm/wantused.htm
thanks Don Chernoff
Advanced Surface Microscopy, Inc. E-Mail: asm-at-indy.net 6009 KNYGHTON RD. Voice: 317-251-1364 INDIANAPOLIS IN 46220 Toll free: 800-374-8557 (in USA) web: http://www.a1.com/asm Fax: 317-254-8690 (note: "a1"= letter "a", numeral "1")
I am looking for a postdoc/visiting scientist who will do developmental work on Direct Methods for structure determination using dynamical electron diffraction data. If you know of anyone who has: a) A good diffraction background b) A good crystallography background c) Good computer skills Please ask them to contact me. The position is available now.
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
I just got an Amray 1600 with turbo and would like to correspond with others who are using this instrument. If there are any quirks about it, I would like to know what they are. If anyone has brought one up after several years of storage, I'd REALLY like to hear from you.
I'm hoping to be able to take 4x5" sheet film pix and 120 roll film pix.
Any info on the care and feeding of the turbo pump, roughing pump and inevitable column cleaning would be appreciated. I'm told that this SEM can take good pictures. I'm hoping to find out if this is true.
Having serviced Siemens microscopes I can tell you that the source of th= e mercury is a mercury diffusion pump. This pump is situated between the rotary pump and the "oil" diffusion pump in the vacuum circuit.
So that is where the mercury is now to dispose of it is another problem?
Stay safe.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
I am currently in the process of acquiring a TEM with accessory equipment. We are starting a new TEM-lab here at the Swedish Museum of Natural History.
One of the instruments I am considering is the LEO 912 AB. Anyone on the list with experience of that microscope? We will primarily be doing morphology including ESI imaging of thick sections, but not analytical work or cryo.
Also: any recommendations on which ultramicrotome to choose.
TIA
Ulf Jondelius
Ulf Jondelius, Invertebrate Zoology Swedish Museum of Natural History P.O.B. 50007, 104 05 Stockholm, Sweden phone: Int + 46 (0)8 666 5160 fax: Int + 46 (0)8 666 4125 e-mail: ulfj-at-nrm.se
Here's a few comments to follow from Doug Keene's reply to you celss on coverslips question.
You can process and section Thermanox quite easily with cells grown on them.
Orientation problems can be overcome if you cut the coverslip to fit a BEEM capsule before you innoculate with cells. I have seen good results with various cell types, and if you taper the cut coverslip to fit into the BEEM pot, trimming the block is pretty quick too. If you need, you can also bend up the coverslip at the non-tapered end to indicate which side the cells are growing.
Spurrs resin seems pretty good, but there can be some delamination between the coverslip and resin, but not always!
Hope this helps.
Plymouth Electron Microscopy Unit University of Plymouth Drake Circus Plymouth Devon UK PL4 8AA Tel: 01752 233092 Fax: 01752 233092 email: pbond-at-plymouth.ac.uk
I need to colorize some SEM pictures but I do not know which programm to use. If someone can help me, I use PC or Macintosh computer. Thanks a lot for your help Best regards
Didier
-------------------------------------------- Didier Le Thiec I.N.R.A. Centre de Recherches Forestieres Unite d'Ecophysiologie Forestiere Laboratoire de Pollution Atmospherique 54280 Champenoux - France
To save a long search can anyone remember which months archive of the listserver the discussion about preparing cd-roms for sem is in??
Chris
Chris Gilpin Experimental Officer Biological Sciences EM Unit G452 Stopford Building Oxford Road Manchester M13 9PT phone +44 0161 275 5170 Fax +44 0161 275 5171 Chris Gilpin http://www.empgu.man.ac.uk
I am now running a clinical path, diagnostic EM lab. but must reduce my storage. What are the clia and cap regs on how long to keep transmission em blocks, thick section slides, grids and phot mic's? I am being reduced from a 3 room lab to a room and a closet. thanks for you help. S. Drew
I would like to draw your attention to the materials science symposium being held as part of the Scanning 99 meeting in April this year. The abstract deadline is February 15th (the same as MSA) and the abstract format is the same as the MSA format. It is intended that there will be contributed presentations as part of the program, and depending on the number of submissions, the symposium may be extended for another day. Please forward this e-mail to anyone you think may be interested in attending the symposium.
{bold}
"Analyzing Materials Interfaces at Atomic Resolution" {/bold}
There will be a Materials Science symposium at Scanning 99 entitled "Analyzing Materials Interfaces at Atomic Resolution". Scanning 99 is being held at the Hyatt Regency O'Hare in Rosemont, IL from April 11-14 1999. The "Analyzing Materials Interfaces at Atomic Resolution" symposium is tentatively scheduled for Tuesday April 13th and will consist of invited and contributed presentations. The details of the conference and deadlines/forms for abstract submissions can be found at http://www.scanning.org or details can be requested from Mary Sullivan (e-mail: scanning-at-fams.org, tel:201-818-1010). Registration for members of the Midwest Microscopy and Microanalysis Society are at the reduced rates of $150 for the whole conference or $50 for a single day
The discussion was last September, maybe on into October. I will send you a copy of the summary that I have on hand.
Warren S.
At 02:18 PM 2/5/99 +0000, you wrote: } } Hi all } } To save a long search can anyone remember which months archive of the } listserver the discussion about preparing cd-roms for sem is in?? } } Chris Gilpin } Experimental Officer } Biological Sciences EM Unit } G452 Stopford Building } Oxford Road } Manchester } M13 9PT } phone +44 0161 275 5170 } Fax +44 0161 275 5171 Chris Gilpin } http://www.empgu.man.ac.uk }
Just a reminder:ACS's Applied Optical Microscopy will be held in conjunction with PITTCON, March 5-7, in Orlando, Florida.
This is a three-day, hands-on total immersion workshop. Although this workshop is offered under the American Chemical Society Short Course programming, it is not just for chemists. The curriculum focuses on key techniques to help you be more effective in the lab. Topics range from alignment, care and cleaning to contrast techniques and basic measurement. There is also a special Saturday evening program on video and digital imaging and a full day on qualitative and quantitative Polarized Light Microscopy. Participants are encouraged to bring samples.
For details and registration information, visit the MME website {http://www.MME-Microscopy.com/education} or call the course coordinator, Barbara Foster
Barbara Foster Course Coordinator ACS Applied Optical Microscopy Course c/o Microscopy/Microscopy Education 125 Paridon Street, Suite 102 Springfield, MA 01118 (413)746-6931 Fax: (413)746-9311 email: mme-at-map.com
According to some of the messages I saved, the dates are mid-August and September '98. Hope this helps.
Chris Gilpin wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi all } } To save a long search can anyone remember which months archive of the } listserver the discussion about preparing cd-roms for sem is in?? } } Chris } } Chris Gilpin } Experimental Officer } Biological Sciences EM Unit } G452 Stopford Building } Oxford Road } Manchester } M13 9PT } phone +44 0161 275 5170 } Fax +44 0161 275 5171 Chris Gilpin } http://www.empgu.man.ac.uk
I was just asked to provide the equation for gamma adjustment to image contrast. I had always assumed that it was just
Output = (Input)^gamma i.e. a simple power law.
Thus for gamma = 0.5 you get a square root curve and for gamma = 2.0 you have a parabola. However, the contrast curves in various books I've checked do not appear to be a power law, but look more like circular arcs. Unfortunately these books only describe gamma but do not provide the equation. Are the curves just artist's license or is my understanding of the gamma correction wrong?
If you include Contrast and Brightness, I would expect the general equation to be
Output = B + C*(Input)^gamma
Where B = Brightness and C = Contrast.
Thanks, Henk Colijn Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://web.ceof.ohio-state.edu An optimist believes that we live in the best of all possible worlds. A pessimist fears that this is true.
Following a very successful year, NORAN Instruments is pleased to announce employment opportunities within its Confocal Sales Division.
The position of District Sales Manager will be responsible for all confocal sales activities within a predefined region of the United States.
Functions will include: * Evaluation and qualification of customer needs * Demonstration of confocal systems, including configuration and setup * Coordination of installation and initial user training
Necessary qualifications include: * B.A. or B.S. degree in Natural Sciences. Advanced degree desirable. * One year's hands on experience with light microscopy techniques. * Experience and practical knowledge of Confocal microscopy applications. * Experience with computer image analysis and processing preferred. * Sales experience is highly desirable.
The position requires travel of up to 50% within designated sales region.
For consideration, please fax or e-mail your resume in confidence to:
Could some kind, knowledgable person explain why the addition of calcium = to fixatives (buffered aldehydes, biological material, resin embedding and = TEM) is important? =
Regards,
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org
I am looking for any written materials concerning Reichert MeF = microscope. I have one in mint condition (probably never used), but = without any instructions. The microscope was made after 1954. It is now = disassembled and, although I know more or less how to put it together, I = would rather like to do it according to producer's advices.
I am not sure if my question fits to this mailing list, but I think that = history of microscopy would be of interest for some of us.
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD}
{META content=3Dtext/html;charset=3Diso-8859-2 = http-equiv=3DContent-Type} {META content=3D'"MSHTML 4.71.2016.0"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} I am looking for any = written=20 materials concerning Reichert MeF microscope. I have one in mint = condition=20 (probably never used), but without any instructions. The microscope was = made=20 after 1954. It is now disassembled and, although I know more or less how = to put=20 it together, I would rather like to do it according to producer's=20 advices. {/FONT} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} {/FONT} {/DIV} {DIV} {FONT face=3DArial size=3D2} I am not sure if my question fits to = this mailing=20 list, but I think that history of microscopy would be of interest = for some=20 of us. {/FONT} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} {/FONT} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} Thank you {/FONT} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} {/FONT} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} Pawel = Karaszkiewicz {/FONT} {/DIV} {DIV} {FONT color=3D#000000 face=3DArial size=3D2} {A=20 href=3D"mailto:zekarasz-at-cyf-kr.edu.pl"} zekarasz-at-cyf-kr.edu.pl {/A} {/FONT} {= /DIV} {DIV} {FONT color=3D#000000 face=3DArial = size=3D2} =20 {/FONT} {/DIV} {/BODY} {/HTML}
There is a Win95/WinNT program called EIKONA3D for volumetric image processing, analysis and visualization, which provides special features and tools for working with image sequences originated from microscopy (e.g. 3D image processing, image alignment, 3D reconstruction, 3D visualization, volume rendering, surface rendering, 3D registration, etc.). One of the tools it provides is 3D segmentation of a 3D data set (image sequence) and labeling/colorization of 3D regions. See the site: http://www.alphatecltd.com/eikona3d.html
Best regards, Nikos Nikopoulos
At 11:10 AM 2/6/99 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I have used Adobe PhotoDeluxe which is available (and pretty cheap) for both the Mac and PCs. The program is deceptively simple, but quite usefull if you look into it a bit.
By adding a transparent layer, you can colorize the image without actually modifying it. Once a colored layer is created the colors can be rapidly changed to fit your tastes.
Photoshop will do the same....but for a much higher price, and learning curve.
} } Dear all, } } } } I need to colorize some SEM pictures but I do not know which } } programm to use. If someone can help me, I use PC or Macintosh computer. } } Thanks a lot for your help } } Best regards } } } } Didier } }
-- _______________________________________________ / Michael J. Herron / / U of MN,Medicine/Infectious Diseases / / herro001-at-maroon.tc.umn.edu / / http://128.101.243.213 / /__________________________________________/
I have some samples of muscle tissue in which I would like to determine the distribution of mitochondria within each myocyte.
My life would be made easier, and the sample size bigger, if I could do this at the LM level rather than in the TEM. I figure that the mitochondria will be visible in the LM because they occur in large groups in this tissue, rather than singly. Also, I don't need to see every mitochondrion, just the general pattern.
The question is: does anyone know of a suitable specific stain? I am after a bright-field permanent stain that will work on semi-thin resin sections, not a fluorescent or antibody method. A nice old-fashioned stain!
So far the tissue has been fixed in glutaraldehyde but not processed further. Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department Faculty of Medicine & Health Sciences University of Auckland Private Bag 92019 Auckland New Zealand
I'm studying the transport and localization of some proteins in the cytoplasm of cultured cells. The cells are fixed in paraformaldehyde and stained with a avidin-biotin-fluorescein system. I need to do double staining for this protein and the Golgi, so I intend to use the Bodipy-tr-ceramide probe, as suggested by Molecular Probes .
I would like to know if someone has experience or worked with this specific probe. Particularly I need to know if this probe may be used with fixed cells, that is, after the cells are fixed.
I will be very grateful for any information
Thanks in advance
Francisco Javier Hernandez-Blazquez IARC-WHO Unit of Multistage Carcinogenesis 150 Cours Albert Thomas Lyon - France
I have copies of the operations manuals for both the MeF3 and for its camera system and would be happy to provide you with copies. There would be a slight fee for copying and postage. Please let me know if you are interested.
Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.
} } } }
{excerpt}
{fontfamily} {param} Arial {/param} {smaller} I am looking for any written materials concerning Reichert MeF microscope. I have one in mint condition (probably never used), but without any instructions. The microscope was made after 1954. It is now disassembled and, although I know more or less how to put it together, I would rather like to do it according to producer's advices.
{/smaller} {/fontfamily}
{fontfamily} {param} Arial {/param} {smaller} I am not sure if my question fits to this mailing list, but I think that history of microscopy would be of interest for some of us.
{/smaller} {/fontfamily}
{fontfamily} {param} Arial {/param} {smaller} Thank you
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I'm studying the transport and localization of some proteins in the cytoplasm of cultured cells. The cells are fixed in paraformaldehyde and stained with a avidin-biotin-fluorescein system. I need to do double staining for this protein and the Golgi, so I intend to use the Bodipy-tr-ceramide probe, as suggested by Molecular Probes .
I would like to know if someone has experience or worked with this specific probe. Particularly I need to know if this probe may be used with fixed cells, that is, after the cells are fixed.
I will be very grateful for any information
Thanks in advance
Francisco Javier Hernandez-Blazquez IARC-WHO Unit of Multistage Carcinogenesis 150 Cours Albert Thomas Lyon - France
How can you run a lab with the space you are getting? Everytime we are = CAP inspected we are cited (non binding) for lack of space. We have 5 = large rooms that are used for clinical purposes. Fight with the powers = that be on this CAP requirement as opposed to throwing out clinical = specimens.(ie blocks, slides, etc.)
If more room is not posible, how about off site storage? If I had to, I = could store blocks, prints, negs, thicks, etc in a 10 x 10 foot room. = (not much room to move in ) that we have collected in the lab since 1974. = =20
I do not actually now the real requirements that you are asking about, but = check with the histology lab and use those rules as guidelines.
Best of Luck,
Ed
} } } Sharon Drew {drewsh-at-smtpgw2.musc.edu} 02/05 9:45 AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html=20=
I am now running a clinical path, diagnostic EM lab. but must reduce my storage. What are the clia and cap regs on how long to keep transmission em blocks, thick section slides, grids and phot mic's? I am being reduced from a 3 room lab to a room and a closet. thanks for you help. S. Drew = = = = = = = = = = =20
I have some samples of muscle tissue in which I would like to=20 determine the distribution of mitochondria within each myocyte.=20
My life would be made easier, and the sample size bigger, if I could=20 do this at the LM level rather than in the TEM. I figure that the=20 mitochondria will be visible in the LM because they occur in large=20 groups in this tissue, rather than singly. Also, I don't need to=20 see every mitochondrion, just the general pattern.
The question is: does anyone know of a suitable specific stain? I am=20 after a bright-field permanent stain that will work on semi-thin resin=20 sections, not a fluorescent or antibody method. A nice old-fashioned=20 stain!
So far the tissue has been fixed in glutaraldehyde but not processed=20 further. Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department Faculty of Medicine & Health Sciences University of Auckland Private Bag 92019 Auckland New Zealand
Hi Paul, During aldehyde fixation the calcium ions tend to leach out, thus the addition of CaCl2 to the buffer solution. Of course there are other important factors to consider such as pH, osmolality, and choice of buffer (PIPES, MOPES, or Cacodylate instead of Phosphate which may result in some percipitation). MgCl2,and KCl are are also sometimes added; depending on what is important to preserve. (Gronblad,M., (1983) Cell Tissue Res. 229,627 and Glauert, A.M., 1975. Fixation, dehydration, and embedding of Biological specimens. Practical Methods in Electron Microscopy. Amer. Elsevier Pub. Co.,Inc., New York 207pp.
At 10:41 PM 2/5/99 -0800, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
This is a multi-part message in MIME format. --------------52E001D5E7420FECEBB67733 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit
Hi all I am wondering if there is any source for SEM images that can be downloaded as "pdf" files. The images are for corroded metals. Regards Mayouf
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Hello all,
I would like to demonstrate the juxtaglomerular cells(=myoepitheloid cells) of kidney on semi-thin sections by using different staining methods. I remember reading a very nice method to show these cells. However, I can not find it now.
I will greatly appreciate if you could send me the suitable staining methods which you used before and also your suggestions about that.
Thanks in advance.
Best regards,
Ranan Gulhan AKTAS, M.D. Trakya University, Faculty of Medicine Pathology Department Edirne 22030 Turkey
Henk, I think that your equation is wrong. The gamma correction is Out = (In) ^(1/gamma). This gives a parabola (upwards turning) for gamma=1/2 which will expand the contrast in the bright region at the expense of the dark regions of the image. For gamma=2, it is a square root function (also a parabola on its side) that will expand the contrast in the dark regions at the expense of the light region. I assume that you would normalize the output range so that it was 0 to 255 with a suitable coefficient in the equation. The curves should go through 0 and 255, so there is no offset constant in the equation. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
---------- } From: Hendrik O. Colijn To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Hi all,
I was just asked to provide the equation for gamma adjustment to image contrast. I had always assumed that it was just
Output = (Input)^gamma i.e. a simple power law.
Thus for gamma = 0.5 you get a square root curve and for gamma = 2.0 you have a parabola. However, the contrast curves in various books I've checked do not appear to be a power law, but look more like circular arcs. Unfortunately these books only describe gamma but do not provide the equation. Are the curves just artist's license or is my understanding of the gamma correction wrong?
If you include Contrast and Brightness, I would expect the general equation to be
Output = B + C*(Input)^gamma
Where B = Brightness and C = Contrast.
Thanks, Henk Colijn Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://web.ceof.ohio-state.edu An optimist believes that we live in the best of all possible worlds. A pessimist fears that this is true.
I am looking for public domain images from electron microscopes or anyone in the New York area that might be interested in collaborating with a design firm to produce a high profile television spot. Any help would be appreciated..
Jeff Linnell Liquid Design Group jeff-at-liquidesign.com
My laboratory is equipped with a LEO 912 Omega, the model before the AB if I'm not mistaken, which was purchased 4 years ago. This is an excellent instrument, powerful, versatile and dependable. I use it mostly for conventional and cryo-EM with excellent results. I would reccomend it without hesitation.
For microtome, I have been a satisfied Reichert Ultracut user for years, which is why I bought an Ultracut S for my lab 4 years ago. The fully motorized controls needed getting used to in the beginning but have proven remarkably reliable.
Usual disclaimer: I have no interest or relation to either company other than being a very satisfied customer.
If you have specific questions, don't hesitate to contact me.
Regards, Michel **************************************************** Michel Deschuyteneer, Ph.D. deschuyt-at-sbbio.be Scientist Electron Microscopy Laboratory
SmithKline Beecham Biologicals Rue de l'Institut, 89 B1330 Rixensart, BELGIUM Tel: +32-2-656 9290 Fax: +32-2-656 8164 **************************************************** Standard disclaimer: the opinions expressed in this communication are my own and do not necessarily reflect those of SmithKline Beecham. ****************************************************
Never used one of those, so I don't know if these are possible after fixation in glut_de. At least some are possible, acc. to the text, after fixation in "formaldehyde-based fixatives" and "postfixation" in potasium dichromate...
These are all described in Langeron, M: "Precis de microscopie", Masson, Paris, 1934...
Can send you a copy of the protocols if you want (*.tif, *.jpg, whatever...it's in French).
Yvan Lindekens.
---------- } Van: Stephen Edgar {s.edgar-at-auckland.ac.nz} } Aan: Microscopy-at-sparc5.microscopy.com } Onderwerp: LM: A stain for mitochondria? } Datum: maandag 8 februari 1999 5:13
} I have some samples of muscle tissue in which I would like to } determine the distribution of mitochondria within each myocyte. } } The question is: does anyone know of a suitable specific stain? I am } after a bright-field permanent stain that will work on semi-thin resin } sections, not a fluorescent or antibody method. A nice old-fashioned } stain! } } So far the tissue has been fixed in glutaraldehyde but not processed } further. } Regards } } Stephen Edgar
Our group has inherited a Boston-Bradley Adjustable Blade manufactured by Gardener Laboratory of Bethesda, MD. During an on-line search we were able to determine that the company existed in 1952, but no other information was available.
We believe this to be a crude microtome. It has a stainless steel "clamp" and brass inserts that are labeled with exact thickness from 0.001 to 0.101mil. The clamp is a rectangular block with 3/4" on each end 5mm higher than the center section
Profile ____ ____ ____________
Along one side of this center section is an adjustable stainless steel slab with an arrow pointing to the center of one edge. The knurled knobs are on the other side of the center section. This slab may be adjusted such that it is above or below the center height. It may also be loosened from the center area so that it is a distance from the center.
It seems that the only way to cut would be to raise the slab, place a specimen on the center area (top down), hold the specimen in place and cut it off with a razor blade. This seems upside-down to every other microtome I have dealt with.
Has anyone ever used one of these? We need a crude sledge microtome and this may fit our needs if we could just figure out how to use it.
I had always been told that it helped to preserve the membranes. I've never tested that information, however.
Bob Underwood Derm Research Center U of Washington
On 5 Feb 1999, Webster wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, } } Could some kind, knowledgable person explain why the addition of calcium to fixatives (buffered aldehydes, biological material, resin embedding and TEM) is important? } Regards, } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } phone:213 273 8026 } fax: 213 413 6739 } e-mail: pwebster-at-hei.org } } }
} I am looking for public domain images from electron microscopes or } anyone in the New York area that might be interested in collaborating } with a design firm to produce a high profile television spot. Any help } would be appreciated.. } } Jeff Linnell
Jeff -
You'll find a wide variety of images hotlinked at the end of the "Microscopy for Children" bibliography on the Project MICRO web page (URL below). Don't miss the secondary set of links available at "K-12 microscopy resources". Some may be public domain, some are not; you need to check after you've found what you want. There's a stock photo CD-ROM (colorized SEM) available from Corel; it's also in the bibliography .
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
MSA List Recipients, If anyone in possession of a spare Philips CM or EM400 series specimen holder (rod), either regular, low-background, or double-tilt, is willing for a nominal fee or goods and services to part company with said device, please contact me immediately at any of the numbers below. I will be more than appreciative and most remunerative. Thanks. Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W Wiggins, Supervisor 2/9/99 11:25:46 AM CRC-Electron Microscopy Lab. Ofc:704/355-1267 Carolinas Medical Center Fax:704/355-7648 P.O. Box 32861 Lab:704/355-7220 Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Polymer Microscopist - Freeport, Texas and Midland, } Michigan } } Company: The Dow Chemical Company } } Location: Freeport, Texas and Midland, Michigan } } Qualifications (education, certification, language, etc.) and } Experience required: } A candidate with a BS or MS degree in polymer science, material } science or chemistry is preferred with some prior experience in } electron microscopy. } Good written and oral communication skills and the ability to work } both independently and in a team environment are extremely important. } } Job Overview: } } The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D } Analytical Science Laboratory has two professional level full time } openings for Polymer Microscopists, one position at each of the Dow's } facilities in Midland, MI and Freeport, TX. The primary } responsibilities include working with partners to support research } projects involving new and existing products in Dow's polymer } businesses. } } Key responsibilities will include: } } 1. Extensive problem solving. } 2. Microscopy preparation technique experience including } ultramicrotomy and cryo-ultramicrotomy. } 3. Operation of transmission electron microscope. } 4. Interpretation of images. } 5. Documentation of work. } 6. Compliance with safety and quality programs. } 7. Active member of project and SMX work teams. } } Interested: } Please e-mail or send your resume and cover letter, with reference to } this ad to: } Email: R&D-at-Dow.com or The Dow Chemical Company, Workforce } Planning 98-289, P. O. Box 150, Plaquemine, LA 70765. E-mail } respondents must list Job 98-289 and their last name as the first and } second items on the Subject line. Only those selected for an } interview will be contacted. Only U.S. citizens or aliens who are } authorized to work in the United States will be considered for } employment. } } We are an equal opportunity employer and offer a competitive } compensation and benefits package including 401k, stock purchase, } tuition reimbursement and performance incentives. The Dow Chemical } Company is the fifth largest chemical company in the world with annual } sales of US$20billion. Dow manufactures and supplies chemicals, } plastics and agricultural products for customers in 164 countries and } employs approx. 43,000 people worldwide. For more news and } information about Dow, please visit our web site at www.dow.com. } } Bob Cieslinski } Microscopy & Microanalysis } 1897 E Bldg. } (517) 636-6875 }
} } MSA List Recipients, } If anyone in possession of a spare Philips CM or EM400 series } specimen holder (rod), either regular, low-background, or } double-tilt, is willing for a nominal fee or goods and services } to part company with said device, please contact me immediately } at any of the numbers below. I will be more than appreciative } and most remunerative. Thanks. } Winston } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Winston W Wiggins, Supervisor 2/9/99 11:25:46 AM } CRC-Electron Microscopy Lab. Ofc:704/355-1267 } Carolinas Medical Center Fax:704/355-7648 } P.O. Box 32861 Lab:704/355-7220 } Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ }
-----------------End of Original Message-----------------
can anyone tell me if people still use BSA or bacitracin as 'wetting agents' when negative staining? I cannot find reference to them in any of my texts but I have used them since the dawn of time, so I think I have just lost the references and/or they've gone out of fashion.
If anyone has advice on use, advantages of particular chemicals or references I would be grateful.
thanks
Malcolm
PS our glow discharge unit doesn't work - so I can't use that.
Malcolm Haswell Electron Microscopy School of Sciences Fleming Building University of Sunderland SUNDERLAND SR1 3SD Tyne and Wear UK
Tel (0191) 515 2872 e-mail: malcolm.haswell-at-sunderland.ac.uk
} } MSA List Recipients, } If anyone in possession of a spare Philips CM or EM400 series } specimen holder (rod), either regular, low-background, or } double-tilt, is willing for a nominal fee or goods and services } to part company with said device, please contact me immediately } at any of the numbers below. I will be more than appreciative } and most remunerative. Thanks. } Winston } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Winston W Wiggins, Supervisor 2/9/99 11:25:46 AM } CRC-Electron Microscopy Lab. Ofc:704/355-1267 } Carolinas Medical Center Fax:704/355-7648 } P.O. Box 32861 Lab:704/355-7220 } Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ }
-----------------End of Original Message-----------------
We have an "Image Galleries" page on the WWW-Virtual Library for microscopy site where you may be able to find something suitable. http://www.ou.edu/research/electron/www-vl/image.shtml
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
Jeff wrote:
} I am looking for public domain images from electron microscopes or } anyone in the New York area that might be interested in collaborating } with a design firm to produce a high profile television spot. Any help } would be appreciated.. } } Jeff Linnell
Jeff -
You'll find a wide variety of images hotlinked at the end of the "Microscopy for Children" bibliography on the Project MICRO web page (URL below). Don't miss the secondary set of links available at "K-12 microscopy resources". Some may be public domain, some are not; you need to check after you've found what you want. There's a stock photo CD-ROM
(colorized SEM) available from Corel; it's also in the bibliography .
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
Hi folks, it has been a while but I am back and of course I need help.
I should submit a proposal to my Dean and Chancellor on a potential centralized morphology core (CMC). They want to see numbers for outlay-cost-income, and I am having trouble coming up with any cost about structure, maintenance.
I will very much appreciate either a brief response to the points below or someone pointing pointing me to a source for this information on the web (phone numbers or email addresses of members with such information will be appreciated). I am envisioning a CMC to provide under one roof: TEM, SEM, Digital Imaging, Confocal, Atomic Force Microscopy and ancillary services such as frozen, histo, immuno-histo and photography. I realize that information for all these may come from different places. Overall, what I need is just an approximate idea for:
1) Cost to operate existing facilities in academic centers?
2) What is the cost for maintaining equipment present at those facilities and to upgrade components?
3) What is the cost to internal and outside users? -Do you charge per case, number of blocks, number of samples, number of cuts?
-How many prints do you provide and at what magnifications for each case?
-Do you retain negatives and when you do not, do you charge extra?
-Charges for embedding cutting and staining frozens, paraffin?
-Charges for plastic processing (JB-4, historesin, etc), sectioning and staining?
-Charges for dupplicating slides, making slides, prints,
-Charges for preparing PhotoShop publication quality composites on color sublimation paper?
-Charges for using microscopy equipment
-Light without and with phase, Normaski, fluorescence, etc.
-Digital confocal optical sectioning, reconstruction, etc.
4) Exceptions.
a) Do junior faculty get service for less than senior and funded faculty?
b) Are there internal mechanisms for covering the costs of promising-emerging faculty, but without active support?
c) How many places have internal mechanisms such as the now extinct NIH BSRG to cover costs?
d) Are any of those costs derived directly or indirectly from grant overheads?
5) Space now housing the facility you describe?
6) How many of the facilities started with external funds?
7) How many of the facilities started with Dean or Chancellor funds?
8) Any other information I miss, but you consider important when considering a CMC?
9) In particular I want to show that most CMC DO NOT make a profit, because of the huge costs for maintenance contract on equipment???
10) Is there anyone out there getting internal support from grant's overhead, and is the money used for CMC considered an incentive-kick-back to funded invetigators?
11) How many of the existing CMC out there offer Cell Sorting (flow) as part of the morphology services?
-Arrangements?
-Maintenance?
-Support?
-Internal and external charges?
Thanks a lot. I will collate and post the results.
*Disclaimer: Whatever... is not Tulane opinion! Cesar D. Fermin, Ph.D. Professor of Pathology & Otolaryngology web:[ http://www.tmc.tulane.edu/ferminlab/] or [http://www.tmc.tulane.edu/imaging/] Internet: [cfermin-at-mailhost.tcs.tulane.edu] 1430 Tulane Ave/SL79 New Orleans, La 70112-2699 Fax 504 587-7389, Voice 584-2521, Main Office 588-5224
The Michigan Microscopy and Microanalysis (MMM) Society will hold its Spring Meeting on May 7, 1999 at the Genoa Woods Conference Center, 7707 Conference Center Drive, Brighton, MI. The program chair is still soliciting additional student papers for the meeting. If interest or you would like more details on the meeting contact the local affiliate president, Rob Eversole email at eversole-at-wmich.edu.
MSA (the Microscopy Society of America) is making a concerted effort to provide a forum for multiphoton imagers to gather for candid discussions concerning the new technique.
This year's event is condensed to a one day symposium,
#31 Multi-Photon Excitation Microscopy: the Next Generation 2 or 3 AUG 99 in Portland, OR
This year there will be far less time available; however, there are several important goals for this symposium: 1) invite all new speakers for the various applications presentations 2) presentations describing the commercial systems currently available 3) provide a question and answer session forum for technique education
Poster and abstract submissions are welcome, but please hurry since the deadline for abstract inclusion is 15 FEB 99. See http://www.msa.microscopy.com/
The morning session covers a variety of strong applications of (currently quite expensive) ultra-short pulse laser based fluorescence microscopy...
Karel Svoboda, Ph.D Cold Spring Harbor Laboratory "2PELSM for High-Resolution Imaging in Scattering Biological Tissues: Applications to Neuroscience"
Jayne Squirrell University of Wisconsin "2PEFM of Cytoplasmic Organization in Living Mammalian Embryos"
Mary Dickinson, Ph.D. Caltech "Biological Applications of Chromophores With Large Two-Photon Cross-Sections"
Christopher Navara, Ph.D Wayne Hughes Institute "Dynamic Drug Effects Monitoring"
Paul M W French, Ph.D Imperial College of Science, Technology and Medicine "Fluorescence Lifetime Imaging of Biological Tissue"
Afternoon question/answer session panel: to include the morning speakers, plus
Dave Piston, Ph.D Vanderbilt University Warren Zipfel, Ph.D Cornell University Rebbeca Williams Cornell University Vickie Centonze-Frohlich, Ph.D University of Wisconsin John White, Ph.D University of Wisconsin
In addition, we plan to have vendor presentations and handouts for the commercially available two (multi) photon systems.
==============================================
David L. Wokosin Instrumentation Development Engineer Integrated Microscopy Resource University of Wisconsin-Madison 1675 Observatory Drive Madison, WI 53706-1205
******************************************************************************** Victoria Centonze Frohlich, Ph.D. Deputy Director, IMR University of Wisconsin, Madison P(608) 263-6288 F(608) 265-4076 http://www.bocklabs.wisc.edu/imr/home.htm ******************************************************************************** --============_-1293529032==_ma============ Content-Type: text/enriched; charset="us-ascii"
MSA (the Microscopy Society of America)
is making a concerted effort to provide a forum for multiphoton
imagers to gather for candid discussions concerning the new technique.
This year's event is condensed to a one day symposium,
{bold} #31 Multi-Photon Excitation Microscopy: the Next Generation
{/bold} 2 or 3 AUG 99 in Portland, OR
This year there will be far less time available; however, there are several important goals for this symposium:
1) invite all new speakers for the various applications presentations
2) presentations describing the commercial systems currently available
3) provide a question and answer session forum for technique education
Poster and abstract submissions are welcome, but please hurry since the deadline for abstract inclusion is 15 FEB 99. See http://www.msa.microscopy.com/
The morning session covers a variety of strong applications of (currently quite expensive) ultra-short pulse laser based fluorescence microscopy...
Karel Svoboda, Ph.D Cold Spring Harbor Laboratory
"2PELSM for High-Resolution Imaging in Scattering Biological Tissues:
Applications to Neuroscience"
Jayne Squirrell University of Wisconsin
"2PEFM of Cytoplasmic Organization in Living Mammalian Embryos"
Mary Dickinson, Ph.D. Caltech
"Biological Applications of Chromophores With Large Two-Photon Cross-Sections"
Christopher Navara, Ph.D Wayne Hughes Institute
"Dynamic Drug Effects Monitoring"
Paul M W French, Ph.D Imperial College of Science, Technology and Medicine
"Fluorescence Lifetime Imaging of Biological Tissue"
Afternoon question/answer session panel:
to include the morning speakers, plus
Dave Piston, Ph.D Vanderbilt University
Warren Zipfel, Ph.D Cornell University
Rebbeca Williams Cornell University
Vickie Centonze-Frohlich, Ph.D University of Wisconsin
John White, Ph.D University of Wisconsin
In addition, we plan to have vendor presentations and handouts for
the commercially available two (multi) photon systems.
Cesar- you mention what you envision in a CMC what equipment do you currently have? what equipment will you be purchasing? I have used NIH microscopy centers, and I have managed institutional microscopy facilities. The more money you have for staff the better your facility will operate. It is best if you have one staff member per microscope. It gets crazy if your staff is over worked. You will find maintenance costs will be lower, the quality of results will improve, and overall costs will decrease. Maintenace contracts are a killer, we were once paying over $95,000 per year on service contracts for 4 EM scopes. It was driving prices through the roof, try to negotiate with the manfacturers. If you buy all new scopes, maybe buy from one vendor, and see if they will throw in a service engineer as part of the package, or hire a maintenance person yourself. Try to set costs in a per protocol fashion avoid itemizing. If you need any more specifics please feel free to contact me directly. -Mike
On Tue, 9 Feb 1999, Cesar D. Fermin Ph.D. wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi folks, it has been a while but I am back and of course I need help. } } I should submit a proposal to my Dean and Chancellor on a potential } centralized morphology core (CMC). They want to see numbers for } outlay-cost-income, and I am having trouble coming up with any cost about } structure, maintenance. } } I will very much appreciate either a brief response to the points below } or someone pointing pointing me to a source for this information on the } web (phone numbers or email addresses of members with such information } will be appreciated). I am envisioning a CMC to provide under one roof: } TEM, SEM, Digital Imaging, Confocal, Atomic Force Microscopy and } ancillary services such as frozen, histo, immuno-histo and photography. } I realize that information for all these may come from different places. } Overall, what I need is just an approximate idea for: } } 1) Cost to operate existing facilities in academic centers? } } 2) What is the cost for maintaining equipment present at those facilities } and to upgrade components? } } 3) What is the cost to internal and outside users? } -Do you charge per case, number of blocks, number of samples, number of } cuts? } } -How many prints do you provide and at what magnifications for each } case? } } -Do you retain negatives and when you do not, do you charge extra? } } -Charges for embedding cutting and staining frozens, paraffin? } } -Charges for plastic processing (JB-4, historesin, etc), sectioning and } staining? } } -Charges for dupplicating slides, making slides, prints, } } -Charges for preparing PhotoShop publication quality composites on } color sublimation paper? } } -Charges for using microscopy equipment } } -Light without and with phase, Normaski, fluorescence, etc. } } -Digital confocal optical sectioning, reconstruction, etc. } } 4) Exceptions. } } a) Do junior faculty get service for less than senior and funded } faculty? } } b) Are there internal mechanisms for covering the costs of } promising-emerging faculty, but } without active support? } } } c) How many places have internal mechanisms such as the now extinct NIH } BSRG to cover } costs? } } d) Are any of those costs derived directly or indirectly from grant } overheads? } } 5) Space now housing the facility you describe? } } } 6) How many of the facilities started with external funds? } } } 7) How many of the facilities started with Dean or Chancellor funds? } } } 8) Any other information I miss, but you consider important when } considering a CMC? } } } 9) In particular I want to show that most CMC DO NOT make a profit, } because of the huge costs for maintenance contract on equipment??? } } 10) Is there anyone out there getting internal support from grant's } overhead, and is the money used for CMC considered an incentive-kick-back } to funded invetigators? } } } 11) How many of the existing CMC out there offer Cell Sorting (flow) as } part of the morphology services? } } -Arrangements? } } -Maintenance? } } -Support? } } -Internal and external charges? } } } Thanks a lot. I will collate and post the results. } } *Disclaimer: Whatever... is not Tulane opinion! } Cesar D. Fermin, Ph.D. Professor of Pathology & Otolaryngology } web:[ http://www.tmc.tulane.edu/ferminlab/] or } [http://www.tmc.tulane.edu/imaging/] Internet: } [cfermin-at-mailhost.tcs.tulane.edu] } 1430 Tulane Ave/SL79 New Orleans, La 70112-2699 } Fax 504 587-7389, Voice 584-2521, Main Office 588-5224 } } }
Received: from [209.156.13.156] by web507.yahoomail.com; Tue, 09 Feb 1999 16:26:02 PST
Our group has inherited a Boston-Bradley Adjustable Blade manufactured by Gardener Laboratory of Bethesda, MD. During an on-line search we were able to determine that the company existed in 1952, but no other information was available. We believe this to be a crude microtome.
It has a stainless steel "clamp" and brass inserts that are labeled with exact thickness from 0.001 to 0.101mil. The clamp is a rectangular block with 3/4" on each end 5mm higher than the center section
Along one side of this center section is an adjustable stainless steel slab with an arrow pointing to the center of one edge. The knurled knobs are on the other side of the center section. This slab maybe adjusted such that it is above or below the center height. It may also be loosened from the center area so that it is a distance from the center. It seems that the only way to cut would be to raise the slab,place a specimen on the center area (top down), hold the specimen in place and cut it off with a razor blade. This seems upside-down to everyother microtome I have dealt with.
Has anyone ever used one of these? We need a crude sledge microtome and this may fit our needs if we could just figure out how to useit.
Thank-you for your help.
Maureen Hunt BP-Amoco p.l.c.
_________________________________________________________ DO YOU YAHOO!? Get your free -at-yahoo.com address at http://mail.yahoo.com
We just replaced the filament in our Jeol 2010, with a Denka filament can anyone tell me who the supplier is in australia, or elsewhere, I'd like to buy another. The filament is a Denka LaB6 Cathode LKSH M3 104051
Thanks George
George Theodossiou Dept Applied Physics RMIT GPO Box 2476V Melbourne 3001 Victoria Australia Ph: +61 3 9925 3394 Fax: +61 3 9925 5290
We just replaced the filament in our Jeol 2010, with a Denka filament can anyone tell me who the supplier is in australia, or elsewhere, I'd like to buy another. The filament is a Denka LaB6 Cathode LKSH M3 104051
Thanks George
George Theodossiou Dept Applied Physics RMIT GPO Box 2476V Melbourne 3001 Victoria Australia Ph: +61 3 9925 3394 Fax: +61 3 9925 5290
Leica Microsystems, Diatome US, and Electron Microscopy Sciences, announce another in a series of TEM Specimen Preparation workshops. This seminar will focus on the hands on participation of the following techniques:
Embedding of industrial samples Specimen trimming
Ultramicrotomy of hard materials Ultramicrotomy of polymers
Collection & handling of sections Staining of polymer sections
Low temperature ultramicrotomy
The format of our workshop is half day lecture and half day bench work in small working groups. Video attachments will be used on the ultramicrotomes in order to maximize the teaching experience for all involved. Samples will be supplied by the course instructors. Participants are encouraged to bring their own samples to work with as time allows.
Course Speakers & Instructors
Dr. Tom Malis Mr. Bob Vastenhout CANMET DOW Chemical Characterization Group Leader Polymer Microscopist Materials Technology Laboratory Analytical Science Department Ottawa, Ontario Terneuzen, The Netherlands
Mr. Helmut Gnagi Product Manager Microtomist Extaordinaire Diatome, Ltd., Switzerland
Hosted by: JoAn Hudson Clemson University Microscopy Facility Clemson, SC
When: June 9-11, 1999
Tuition: $1,400.00
Includes three nights lodging at the lakefront Conference Center & Inn at Clemson University, continental breakfast and lunch daily, one group dinner, course supplies, and lab charges.
Contact: Mike Boykin, Leica Microsystems, Inc. 800-248-0665 X5092
If you are or were using Accu-Scope (3001, 3002, 3018PH etc.) or Wang Biomed (3002, 3004) microscopes - please share your opinion about them and your experience with me.
What did you use it for? Did it prove suitable for the task? Did you have any problems with it? Was its optical/mechanical quality OK for you and for the job?
If you had to use technical support, how helpful/quick were they?
If you used other microscopes too - how do they compare?
Depending on how interesting the answers would be to the other list members, you may either post the replies to the list, or e-mail me directly.
Thank you! -- Regards, Uri uri-at-watson.ibm.com -=-=-=-=-=-=- {Disclaimer}
Dear Listmembers, Does anybody use or have used ruthenium tetroxide as a fixative for biological material? Any references? Thanks in advance. Chris vd Merwe.
I wonder if anyone knows a good textbook for EDS of minerals? I used to have one in the lab, but it's gone missing, and I don't remember the title or author(s). It had the mineralogical characteristics of most or all of the common minerals, and included typical EDS spectra for each one. A very valuable book for a lab that does a lot of geological work, and I'd really like to replace it (and maybe chain it to the wall this time!)
F.C. Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
Can anyone recommend a good text on EDS of minerals? We used to have a good one in the lab, which described the characteristics of various common minerals, and showed typical EDS spectra for them. Unfortunately, it's disappeared and I cannot remember the title or author, so I guess it's time for a new one.
F.C. Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
Dear microscopists, Our facility needs to replace our print processor. I have looked at several models and wanted some feedback about what models work well. We are limited on the amount of space we can use. The processor can not be any larger then 40" X 38", needs to be easy to clean and durable. We are considering purchasing the ILFORD 2150, but heard it may have lots of problems.
Cheers Malcolm, Yes, the bacitracin wetting technique is still valid as ever. The reference you want is: D.W. Gregory and B.J.S. Pirie, Wetting agents for electron microscopy of biological specimens. Proc. Fifth European Congress on Electron Microscopy, (1972). 234-235. David Gregory recommended using a minimum concentration of 7.5 ug/cm3 for formvar coated grids and 10 ug/cm3 on carbon coated formvar or carbon grids as a wetting agent. All the best, Henry
----------------------------------------------------------------------------- Malcolm wrote: } } Dear all } } can anyone tell me if people still use BSA or bacitracin as 'wetting agents' } when negative staining? I cannot find reference to them in any of my texts } but I have used them since the dawn of time, so I think I have just lost the } references and/or they've gone out of fashion. } } If anyone has advice on use, advantages of particular chemicals or } references I would be grateful. } } thanks } } Malcolm } } PS our glow discharge unit doesn't work - so I can't use that. } } Malcolm Haswell } Electron Microscopy } School of Sciences } Fleming Building } University of Sunderland } SUNDERLAND SR1 3SD } Tyne and Wear } UK } } Tel (0191) 515 2872 } e-mail: malcolm.haswell-at-sunderland.ac.uk } } } Henry Eichelberger, EM Facility Manager Department of Biological Sciences Binghamton, University
I am working with the Zeiss LSM 510 Confocal microscope, and am having trouble achieving 3D image reconstruction using the 3D for LSM program. Do you have any recommendations, or know of the resources available through which I can learn this technique? I'd appreciate any information you have on the topic!
----------------------------------------- Laurie Wallin Technician UCSD Department of Anesthesiology and Neuropathology 9500 Gilman Drive 0629 La Jolla, CA 92093 (619)534-1339 or 822-3271
} Are there any grants available to purchase microscopes for an elementary } school in California?
On a national level, no; but there may be an announcement from a major corporation soon that could help you. Your best current possibility is a local corporation that supports education. You'll need about $1000; you'll find the rationale for that figure and advice on what to buy on the Project MICRO web page (URL below). Although I can't supply the funding, I CAN help you write a proposal, and I can write a supporting letter (but I'll be on vacation 2/18-3/28). Where are you located? California is a big state!
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
Dear Chris, Ruthenium tetroxide is slow penetrating, but some structures are better preserved by its use. Examples: Madison, K. C., et. al. J. Invest. Dermatology 88 (1987) 714. and Eichelberger, et. al., Proc. Microscopy & Microanalysis 1994, 270. Best regards, Henry ----------------------------------------------------------------------------- Chris vd Merwe wrote:
} From: "Elektronmikroskopie EM 2075 Lab1-5 NW11" {lab1-at-ccnet.up.ac.za} } Organization: University of Pretoria } To: Microscopy-at-Sparc5.Microscopy.Com } Date: Thu, 10 Feb 1999 12:59:24 CAT-2 } Subject: Ruthenium O4 in biology } Priority: normal } X-mailer: Pegasus Mail for Windows (v2.42a) } } Dear Listmembers, } Does anybody use or have used ruthenium tetroxide as a fixative for } biological material? Any references? } Thanks in advance. } Chris vd Merwe. } } Henry Eichelberger, EM Facility Manager Department of Biological Sciences Binghamton, University
} I wonder if anyone knows a good textbook for EDS of minerals? I used to } have one in the lab, but it's gone missing, and I don't remember the title } or author(s). It had the mineralogical characteristics of most or all of } the common minerals, and included typical EDS spectra for each one. A very } valuable book for a lab that does a lot of geological work, and I'd really } like to replace it (and maybe chain it to the wall this time!)
} F.C. Thomas } MicroAnalysis Facility } Geological Survey of Canada (Atlantic) } Bedford Institute of Oceanography } Dartmouth, Nova Scotia } Canada B2Y 4A2
Perhaps you're thinking of "SEM Petrology Atlas" by Joann E. Welton, Chevron Oil Field Research Company, published by the American Association of Petroleum Geologists, 1984. And no, you can't have my copy! Don't know if it's still in print, but amazon.com will hunt around for a used copy for you (for a small fee, of course).
Cheers, eh?
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
Patricia, Of the many print processors I've used, I'd choose the Ilford above them all. The older versions have a problem blowing fuses but that is supposed to have been corrected with the new models. The only difficulty I had was that with heavy usage, it must be cleaned more often which, for me, was more of a nuisance than a problem. It's easy to use and durable, not to mention relatively inexpensive. Go for it. Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W Wiggins, Supervisor 2/10/99 2:53:24 PM CRC-Electron Microscopy Lab. Ofc:704/355-1267 Carolinas Medical Center Fax:704/355-7648 P.O. Box 32861 Lab:704/355-7220 Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Today I was realesed online free Metallographic Etch's Database. This database is designed to allow you to quick find etchant via powerfull keyword search engine written in Perl. Here are some key features:
1763 records in the database 75 etchants for macro etching 1359 etchants for micro etching 1103 etchants for chemical etching 218 electrolytes for electrolytic etching 75 etchants for physical etching 329 electrolytes for electropolishing
Keyword search engine Browse database by macroetching Browse database by microetching Browse database by chemical etching Browse database by electrolytic etching Browse database by physical etching Browse database by electropolishing Powerfull online help
For more information please see link at microscopy vendors database: http://www.kaker.com/mvd/vendors.html
Henrik Kaker -- SEM-EDS Laboratory Metal Ravne Slovenia http://www2.arnes.si/guest/sgszmera1/index.html
} Can anyone recommend a good text on EDS of minerals? We used to have a good } one in the lab, which described the characteristics of various common } minerals, and showed typical EDS spectra for them. Unfortunately, it's } disappeared and I cannot remember the title or author, so I guess it's time } for a new one. } } F.C. Thomas
Frank,
I think the book you are referring to is SEM Petrology Atlas by Joann E. Welton. It was published in 1984 by The American Association of Petroleum Geologists, Tulsa, OK 74101. ISBN 0-89181-653-4.
Hope this helps. Lou Ross Senior Electron Microscope Specialist Room 101 Department of Geological Sciences University of Missouri Columbia, MO 65211 (573) 882-4777 (573) 882=5458 fax www.missouri.edu/~geosclmr/ebaf.html
Patricia, Our facility has had an Ilford 2150 for 8 years. Though it has had breakdowns, repairs are fairly simple. I feel that the service contract is too expensive for what you get, which is someone walking you through a repair over the phone. I'm no mechanic but I've been able to deal with most of the problems myself and Iford will sell parts if you need to replace something. The one thing that has made the biggest difference in the trouble free operation of the machine is the addition of a dirt/rust filter on the water supply line. After we did that we really have had no problems except for an occassional plumbing leak (also fixable if you can operate a wrench). You'll also find that you don't need to change the chemicals as often as the manufacturer suggests, as they can be expensive. If you need any more information don't hesitate to contact me.
Mary Gail Engle Electron Microscopy & Imaging Facility University of Kentucky Medical Center
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A colleague and I built a device for doing something similar when I was in graduate school. It consisted of an ultrasonic nebulizer to produce the aerosol at one end of a column, N2 carrier gas (low velocity) to move the aerosol and external heaters to heat the column (along with a few other bells and whistles). Samples were collected on grids at the exit end and the whole thing sat in a hood. The path length was about 2m.
What I'd like to know is if you take any care to get a more uniform aerosol size? Do you work at low enough concentrations to have mostly one (or fewer) particles per drop?
|---------------------------------------------------------------| | Opinions expressed are mine an not those of Rohm and Haas Co. | |---------------------------------------------------------------| | Dr. John R. Reffner | rsrj2r-at-rohmhaas.com | | Rohm and Haas Co. | | | 727 Norristown Road | voice:215-619-5283 | | Spring House, PA 19477 | Fax: 215-619-1607 | |---------------------------------------------------------------|
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Hi
The best way I know of preparing pigments for TEM analysis is by blowing an aerosol of the sample dispersed in alcohol/water solution over a hot plate and collecting the sample on a grid placed at the far end of the hot plate. The company I work for is concerned about the safety issues surrounding this so does anybody know of any less hazardous sample preps. or a simple containment facility for the aerosol spray? I thought about building a glass box for containment but are there any commercially available containment/sample prep. units?
Thanks
Brian Manzor e-mail: brian.manzor-at-grmouth.zeneca.com
Received: from nmho05u.rohmhaas.com ([136.141.252.23]) by ima1.rohmhaas.com with SMTP (IMA Internet Exchange 3.11) id 000E8E24; Wed, 3 Feb 1999 08:41:25 -0500 Received: by nmho05u.rohmhaas.com; id IAA09730; Wed, 3 Feb 1999 08:42:12 -0500 (EST) Received: from sparc5.microscopy.com(206.69.208.10) by nmho05u.rohmhaas.com via smap (3.2) id xma009705; Wed, 3 Feb 99 08:42:01 -0500 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id FAA06651 for dist-Microscopy; Wed, 3 Feb 1999 05:19:30 -0600 Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id FAA06644 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Wed, 3 Feb 1999 05:18:59 -0600 Received: from apsn05.zeneca.com (apsn05.zeneca.com [193.132.159.6]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id FAA06637 for {microscopy-at-msa.microscopy.com} ; Wed, 3 Feb 1999 05:18:45 -0600 Received: from apsn15.ukap.zeneca.com by apsn05.zeneca.com (SMI-8.6/SMI-SVR4) id LAA01931; Wed, 3 Feb 1999 11:33:45 GMT Received: by apsn15.ukap.zeneca.com; id LAA29776; Wed, 3 Feb 1999 11:31:39 GMT Received: from ukapzcmsxhub01.ukap.zeneca.com by gbzhp02.ukap.zeneca.com with ESMTP (1.40.112.8/16.2) id AA200221316; Wed, 3 Feb 1999 11:28:36 GMT Received: by ukapzcmsxhub01.ukap.zeneca.com with Internet Mail Service (5.5.2232.9) id {1F6WTK3F} ; Wed, 3 Feb 1999 11:27:36 -0000 Message-Id: {21E55FE938B6D0119A7100805FFEAEC4D0177B-at-UKGR07} } From: Manzor Brian BP {brian.manzor-at-grmouth.zeneca.com} To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-Sparc5.Microscopy.Com}
I'm looking for a gold or graphite/carbon sputter coater for bio specimen preparation.
If you have one to sell, pls let me know details and price.
I'm considering using digital video (DV) for time-lapse/real-time LM. Broadcast/domestic DVcam magazines suggest that DV gives improved resolution over analogue video (S-VHS etc). Added to this, it also seems to give better quality frame by frame editing (+ stills output) when recorded on DV tape (mini or standard) and managed with software like Adobe Premier using Firewire ( aka i.link/IEEE1394) fast serial links.
If anyone has used this technology I'd be interested to hear more about the pros and cons.
TIA
Kevin Jennings -.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. SmithKline Beecham Pharmaceuticals Analytical Sciences -Microscopy Group New Frontiers Science Park Harlow Essex UK -.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.
I am working on the light reflection of mainly squid iridophores. Are there any groups, preferably in the UK, working with polarising and/or interference microscopes who could give me some advice with this matter?
Thank you very much,
Lydia
PS. Hello Daniele ... back to work !!!
Keith Ryan Marine Biological Association of the UK Citadel Hill Plymouth Devon PL1 2PB England
Gary Gaugler, Ph.D. wrote: =============================================== I'm looking for a gold or graphite/carbon sputter coater for bio specimen preparation.
If you have one to sell, pls let me know details and price. ================================================ There are several very excellent data bases of information of who manufactures what in the microscopy and microanalysis market. You might want to consult some of those listings. Two that I myself make frequent use of are the following:
Microscopy Vendors Data Base http://207.137.96.185/mvd/vendors.html
Microworld Resources and New http://www.mwrn.com/
SPI Supplies is one of the several leading manufacturers of this equipment and all the information you would need our units can be found on our website below.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
I can recommend looking at www.bookfinder.com and www.bibliophile.com if you are looking for a used copy of the text (assuming it isn't available new or you are too poor/cheap to pay for it). These are services where you do your own search over a number of used booksellers - I found a couple of technical books I wanted this way and for quite reasonable prices.
Janet Rice MCC Senior Member Technical Staff rice-at-mcc.com 512-338-3266
Yet another lab in search of microtome parts. We have four Leica Ultrotome III ultramicrotomes in very serviceable condition, but a couple need new belts. A supply of spare tubes would also be handy. Is anyone aware of a source for new or used parts for these venerable instruments?
Thanks in advance!
Randy Tindall Electron Microscope Core College of Veterinary Medicine University of Missouri - Columbia Phone: 573-882-8304
Gary: I have a sputter coater for sale. Trouble is I am in Cambridge UK
Patrick Echlin Director, Multi-Imaging Centre School of Biological Sciences Cambridge University
On Wed, 10 Feb 1999, Dr. Gary Gaugler wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I'm looking for a gold or graphite/carbon sputter coater for } bio specimen preparation. } } If you have one to sell, pls let me know details and price. } } } } } } Cheers, } Gary Gaugler, Ph.D. } } PGP Public key: } BDC9 8860 AA83 1FB2 66D3 01BB 9EB8 8E6D 671F BEB3 } }
Steve, We actually have an old LKB Microtome we are getting rid of--you are welcome to it, but we are in Boston. If you want to make arrangements to have it shipped to you--it's yours! Peggy Sherwood MGH-Wellman Labs of Photomedicine 224 50 Blossom Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-3192 (fax)
Patricia, I didn't see your original e-mail request, but saw the responses! We have a Rapidoprint DD3700 Agfa-Gevaert in our lab for processing EM prints. It's very simple, but like some other processors, it has to be cleaned frequently, especially the water stations, depending upon it's use. Peggy Sherwood MGH-Wellman Labs of Photomedicine
Having worked with minerals of many types, I find that a freeware program for the Mac has been extremely useful in predicting spectra of any mineral. DTSA for either 68K or PPC can be obtained from NIST or possibly from the MSA web site (maybe someone else can confirm this). The program can generate synthetic spectra for just about any detector and will simulate thin or thick specimens. It's a wonderful teaching and research tool that I recommend highly.
Ciao for now, Ken
Kenneth JT Livi Department of Earth and Planetary Sciences 34th and Charles Streets Johns Hopkins University Baltimore, Maryland 21218 USA Phone: (410) 516-8342 Fax: (410) 516-7933 e-mail: klivi-at-jhu.edu
} Having worked with minerals of many types, I find that a freeware program } for the Mac has been extremely useful in predicting spectra of any mineral. } DTSA for either 68K or PPC can be obtained from NIST or possibly from the } MSA web site (maybe someone else can confirm this). The program can } generate synthetic spectra for just about any detector and will simulate } thin or thick specimens. It's a wonderful teaching and research tool that I } recommend highly. }
DTSA can be downloaded at http://micro.nist.gov/DTSA/dtsa.html .
----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
Summary and compilation: Tissue cultures and coverslips: Thanks to everyone for the tremendous response to my query. The majority of responses suggested the use of Thermonox coverslips. These may be purchased sterile. One side is prepared for cell adhesion, and is labeled. They are compatible with alcohol, acetone and propylene oxide as solvents, and with Epon, Spurr's and araldite mixtures for resin. These coverslips come in sizes which fit well into tissue culture plates. Other investigators have successfully used glass coverslips, membrane inserts and plastic petri plates. Permanox plates were also recommended, cells may be grown, fixed and embedded in the chambers. Most people use a dip into liquid nitrogen to facilitate removal of the coverslip after embedding. Other simply pull the coverslip off while still warm from the embedding oven. If the coverslip is not removed, it may still be sectioned with a diamond knife, however, the coverslip may separate from the resin under the electron beam. If the coverslip if removed, various methods are suggested to position the cells parallel or perpendicular to the bloc face. A compilation of the responses follows, MANY helpful tricks will be found on these pages. -----------------------------------------------------------------------
I use standard glass coverslips - round ones that fit in 24 and 12 well culture dishes. I sterilize them sitting on a clean filter paper in glass petri dishes then transfer them to culture dishes and seed with cells. I fix and process in 20 ml glass scintillation vials, embed in epon (put coverslip cell side up on a slide with a drop of epon, heat 4-8 hrs at 58 C (timing is important), cross-hatch with a razor blade, slowly lower into liquid nitrogen, and little squares of epon pop off with cells attached. re-embed squares in flat molds and section as usual. Thomas E. Phillips, Ph.D. tphillips-at-biosci.mbp.missouri.edu.
We have had good results with thermonox coverslips and even better with aclar provided the cells will grow on it. Process as usual for TEM and IEM. Heat can curl the aclar sometimes. Scott D. Whittaker sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ The home of " Tips & Tricks " Dear Sally, I too have used Thermanox coverslips for TEM of tissue culture cells, but I found they often pulled out from or wrinkled-up my sections. I had much better luck using filter-membrane inserts. The insert fits into the wells (I used 24-well plates but they come in many sizes). You can even polarize the cell line if necessary by placing media with serum in the well under the insert and without serum in the filter-membrane cup. The membrane then can be fixed and processed in the holder and just before embedding, separate the filter, cut it into pieces, and embed. No wrinkles, no pulling out of the resin (I've used both Spurrs and LR White). Granted, they are more expensive, but. . .
"Tina Schwach" tschwach-at-tc.umn.edu
----------------------------------------------------------- I have used Thermanox coverslips extensively for this purpose. (Check your supplier of Lux tissue culture products, as well as your EM suppliers.) They are resistant to acetone (not sure about propylene oxide) and I've used them with Epon, spurr's and an araldite/epon mixture. They are supplied sterile, and peel off easily from a warm polymerised block, leaving the cells embedded in the resin block. I used to UV sterilise them in a laminar flow hood overnight before use. A word of warning though, they are not suitable for light microscopy, and some cells don't adhere very well to them. As I recall, I have used vero cells and other kidney derived epithelial cell lines on them successfully. You can section thermanox, but I think a better result is obtained by peeling it off, and polymerising some fresh resin over the cells. Contact me with problems if you wish. I have no commercial interest in this product. I've just processed heaps of cultured cells. Good luck, Rosey van Driel {Rosemary.van.driel-at-baker.edu.au}
--------------------- We use the Thermanox coverslips that you can buy from EMS. They work really well you just have to be careful to keep the correct side up (Thermanox has a sidedness to it and the cells only grow right on it). ACLAR works well too, though lately the stuff we buy from Ted Pella seems to be thinner than it used to be & has a tendency to curl up when polymerizing. I you have any questions, please feel free to ask. Paula :-) Paula Sicurello UC Berkeley psic-at-uclink4.berkeley.edu http://biology.berkeley.edu/EML --------------------------------------------------------- Besides the Lux coverslips you can also use membrane inserts made by Falcon or Costar. If you would like have the exact protocol let me know and I can send it to you. I have used them both for number of years now without any problems though I prefer costar inserts. Neelima Shah.............. From: Neelima Shah shahn-at-mail.med.upenn.edu http://www.MED.upenn.edu/morphlab/
---------------------------------------------------------- Visit the lab manual on //con-sgi.microbio.emory.edu/eyes-of-the-eagle/index.html For over ten years our lab has floated monolayers of epithelial celllines from the surface of common plastic culture ware with propylene oxide. This is far easier than trying to use coverslips. Call me at 404 727 3508 if you need some coaching on the technology. Regards, Skip } From: L R MELSEN lmelsen-at-emory.edu http://www.MED.upenn.edu/morphlab/
----------------------------------------------------------------- We routinely attach cells to thermanox coverslips (available from EMS). These coverslips can be sterilized, are compatible with conventional embedding methods and can be thin sectioned with a diamond knife. We fix in GA/FA, osmium, dehydrate and embedd in Spurr's with no problem.
If you must section the coverslip itself, try to arrange it diagonally in the block and pick up the sections on formvar, because the coverslip and the resin tend to separate under the beam.
If you are sectioning parallel to the coverslip, embedd this way. Fill a beam capsule with resin and lay the coverslip, cell side down on top. Polymerize; them immediately upon removing the blocks from the oven, pull the coverslip off. If you do this quickly while the blocks are still warm, the cells will remain on the block and you don't have to worry about sectioning the coverslip. Good Luck, Michelle Peiffer 814-865-212 email:mlk101-at-psu.edu
--------------------------------------------------------------------------- For TEM of cultured cells, we grow the cultures on "Thermanox" tissue culture coverslips. From Nalge Nunc INternational, 50 sterile coverslips, 13 mm in diameter is catalog 174950. EMS also sells these thermanox cover slips in a variety of sizes (see page 143 of their cat XIII). The coverslips can be treated with all the same chemistry as tissue including propylene oxide and Spurrs epoxy, which are two components which solubilize polystyrene. These thermanox coverslips can be sunk cell side up in freshly made Spurrs, then following polymerization the coverslips can be removed by first sawing a small area of the epoxy/cell/substrate, then immersing in liquid nitrogen for a few seconds, then prying away the substrate. Now the embedded cells are immediate on the epoxy. Re-embed two fragments of the culture face to face for cross-sections, or cut the block parallel to the face for tangential sections. We particularly like the round 13 mm thermanox coverslips for immunocytochemistry of cultured cells since they can be floated cell side down in a drop of 100 microlitters antibody - gold conjugate, conserving reagents.
If you would like to grow a larger culture, you could also use "permanox" culture dishes, which are equally resistant to chemicals common in TEM processing. These are also available through EMS and other suppliers.
Douglas R. Keene DRK-at-shcc.org
To process cell cultures for grown on round, 13 mm Thermanox tissue culture cover slips for TEM, we use the following hardware:
For fixation, we use porcelain multi-well dishes. These are what most people refer as "staining dishes". They are white, measure about 3.5 x 4.5" and have 12 depressions. We use these for glutaraldehyde, buffer rinses and OsO4. OsO4 can be completely rinsed from the dishes. Once in the last buffer after OsO4 and prior to ethanols and propylene oxide, we transfer the disks to 50 ml polypropylene culture tubes, such as Fisher 05-539-7 (these are sterile, but certainly it is not necessary to pay for sterile containers). There is plenty of room to enter a pipette for fluid exchange without touching the culture disks, and the culture surface will not touch the walls of the cylindrical tube so there is no worry that the cells will be rubbed off. Given the depth of the tube, we do not worry too much that the culture will dry out as fluids are exchanged, since the atmosphere within the tubes is fairly saturated with solvent vapor. Still, fluid exchange is done quickly. The polypropylene tubes are resistant to P.O. and Spurrs. Do not use tubes made from polystyrene as they will dissolve. Once infiltrated with the last change of epoxy, we fill a resin-resistant container with epoxy to a minimum depth of 5 mm, sink the disk so that the cells face up, then polymerize the epoxy. We steal the polypropylene lids from wheaten snap-cap vials (Fisher #0333520E) which are of the appropriate diameter for embedding 13 mm coverslips. Wearing a dust mask, We use a jewelers saw to free small blocks of embedded culture, loosen the cover slip with liquid nitrogen, then remove the disk which exposes the culture to the surface of the block. We then either re-embedded (in some of the same batch of media which was used to infiltrate the culture) face to face for cross sections, or cut the blocks parallel to the culture surface for tangential sections.
Good luck,
Doug Keene EM Facility Shriners Hospital for Children DRK-at-shcc.org
---------------------------------- Here's a few comments to follow from Doug Keene's reply to you celss on coverslips question.
You can process and section Thermanox quite easily with cells grown on them.
Orientation problems can be overcome if you cut the coverslip to fit a BEEM capsule before you innoculate with cells. I have seen good results with various cell types, and if you taper the cut coverslip to fit into the BEEM pot, trimming the block is pretty quick too. If you need, you can also bend up the coverslip at the non-tapered end to indicate which side the cells are growing.
Spurrs resin seems pretty good, but there can be some delamination between the coverslip and resin, but not always! Hope this helps. P.Bond-at-plymouth.ac.uk
--------------------------------------------
Dear Sally, The coverslips that everyone uses is my Thermanox plastic coverslips which are sterile and come in many different sizes depending on your need. They may be found wither in our hard copy catalog or on our website at http://www.emsdiasum.com. Go to our online catalog and click chapter 7. They are listed under Thermanox. In our hard copy catalog XIII they may be found on page 143. Please let me know if I may be of further assistance to you.
Sincerely, Electron Microscopy Sciences 215-646-1566 } From: "SGKCCK-at-aol.com"-at-sparc5.microscopy.com
I used to grow Vero Cells on thermanox cover slips. In Europe we buy such cover slips through our local distributor " Bioblock", but I am quite sure that you can find the same in the EMS catalog ( Nalg Nunc ref.174950 = tissue coverslip, sterile, thermanox size 13 mm in diameter). In fact the thermanox cover slips are sold sterile and the surface treated for cell culture is on the top (where the label is). I grow Vero cells (or others), infect them, fix them before labelling with gold-coupled antibodies. I forgot to say that I put the cover slip on 24 wells (or 4 wells) adapted plates (from Nunc or Falcon ...). Leaving the coverslip on the well, I do all the dehydration and epon embedding in the same plate. Before polymerization I cut the /\ shape off the beem capsule (and put three of these capsules per well (the uncutted side directly over the specimen) over a thin layer of epon. I polymerize for a night and the next day I fill the beem capsules with epon and polymerize further on. When the samples are polymerized you can just remove the capsules and the cells will be adherent on the epon. As usual you trim your block and cut the sections ( the first one will almost be good). We published this method in EmboJournal 1998, 17, 3899-3908.
I hope that this helps, Daniele Spehner From: "Daniele Spehner" {daniele.spehner-at-etss.u-strasbg.fr}
----------------------------------------------------------- Thermonox cover slips (sold by Fisher Scientific, pg. 1793 of the 98/99 catalog) are great for TEM and SEM applications. They are manufactured by Nunc, come in many different shapes and sizes, have one side textured for cell adhesion, and arrive sterile in packs of 50. They are resistant to acetone, alcohol and can be sectioned using diamond knives (I've heard this, but never tried it) without harming the knife.
I've used them with cultured monkey kidney cells, and the cells stayed attached through critical point drying. We've also coated them with poly-L-lysine and settled fish lymphocytes onto them for SEM (critical point drying didn't remove the cells).
Another neat product is the Nunc SonicSeal Slide System (their number 138121, Fisher Cat. No. 12-565-9, Fisher catalog pg. 1792). Because the wells are made of Permanox, we were able to grow cells, then fix, dehydrate, infiltrate and polymerize right in the chambers. After polymerization we separated the slide from the cells by inserting a razor blade into the interface between the slide and resin and twisting it a little. Good luck, Heather Owen, Director Electron Microscope Laboratory University of Wisconsin - Milwaukee (414)229-681 Owenha-at-csd.uwm.edu
--------------------------------------------------- We are working with Caco-2 cells grown in transwell cultures. We use a protocol similar to the one Tom Moninger describes but we embed in Embed 812 (SOLD BY ems AS A REPLACEMENT FOR Epon 812). We encounter severe wrinkling in both thin and semi-thin sections caused by the cells expanding on the surface of the knife boat while the polycarbonate membrane does not expand. We do not yet have aa remedy for this, but I have asked Tom M. to share his experiences with me. Call if you want more detail. Electron Microscopy Laboratories Email: jcoleman-at-bi-pharm.com {jcoleman-at-bi-pharm.com} ------------------------------------------
Hello Sally, you have some experience with TEM polycarbonate filters and cell culture; and you can use acetone and spurr resin with them, there is no problem at all. Good luck Nuria } From: Nuria Cortadellas {nuriac-at-giga.sct.ub.es} ----------------------------------------------- I have processed many filter membranes for TEM and SEM. I follow standard procedures with the following changes;
-dehydrate in EtOH, not acetone (eats the polycarbonate) -embed in Epon 812 or equivalent (I use Ted Pella's Eponate 12) -times can be shortened conciderably (Fix 20 min, wash and dehydrate steps 5 min, resin changes around 30 min.) -do a couple more resin changes than normal
I carry out the entire process (including embedment) in a 24 well plate and cut the filters out with a jeweler's saw after polymerization.
Thomas Moninger moninger-at-emiris.iaf.uiowa.edu http://www.uiowa.edu/~cemrf -------------------------------------------------- Tom } Hi Tom } Thanks for the quick reply!. Part of my sample may be difficult to fix and } embed, I will probably need longer times for this. Did you recommend } shorter times because of the tissue culture, or is this a requirement for } the filter? } Interesting, someone responded that they use Acetone, however I will play } it safe with alcohol. } And do you have trouble with curling of the sections? } Thanks.........SALLY Exactlly right, I use the short times because I am ususally working on monolayers. You might want to test your filters and plates with the acetone but I would bet you will see some immediate hazing and disolving. Feel free to drop me a note if you have any other questions. ----------------- ----------------- ---------------------------- I am fairly new to microscopy so I'm curious why a researcher needs the cells on coverslips to begin with. In the past when I plated cells for a TEM study I would grow them right in the dish or flask with no coverslip and process the cells right in the dish. You can then embed the cells on the dish and remove the dish after it's cured in liquid nitrogen. Unless I was doing a LM study at the same time and I would then split them right before harvesting. I sterilized the cover slips under the hood with a bunson burner and be very quick. All it takes is a fast wave through the flame, any longer and the coverslips warp. After a few seconds they cooled and then you can put them in the dish. If you don't wait they weld to the dish. You might find sterile coverslips at Fisher, Daeger, VWR or Costar. Good luck and drop me a line if you need more info. Steve D'Angelo From: sdangelo-at-batnet.com (steve d'angelo)
______________________________________________--- MSA QUERY: Original Cover slip embedding Colleagues will be growing Vero cells (Green Monkey Kidney Cells) and intestinal epithelial cells on cover slips for subsequent ultrathin sectioning and TEM examination. I am interested to know which cover slips are best to use for this purpose. Can they be purchased sterile? I have checked the Ted Pella and EMS catalogs, they both sell non sterile cellulose acetate cover slips. Are there compatibility issues with resins and solvents? I am interested in any tips or tricks to smooth the way. Thanks, Sally Burns
Sally Burns Center for Electron Optics burnssal-at-pilot.msu.edu
Try Jan Hinsch at the Leica Allendale office: 201-236-5905
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 11:53 AM 2/11/99 -0500, Joseph Passero wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
If you are or were using Accu-Scope (3001, 3002, 3018PH etc.) or Wang Biomed (3002, 3004) microscopes - please share your opinion about them and your experience with me.
What did you use it for? Did it prove suitable for the task? Did you have any problems with it? Was its optical/mechanical quality OK for you and for the job?
If you had to use technical support, how helpful/quick were they?
If you used other microscopes too - how do they compare?
[In case it matters, I plan to use it for histology, microbiology and hobby, sometimes pushing resolution to the limit, doing mostly visual, once in a while photomicrography. But please share _your_ experience, regardless whether it's in the areas I mentioned, or not.]
Depending on how interesting in your opinion the answers would be to the other list members, feel free to either post the replies to the list, or e-mail me directly.
Thank you! -- Regards, Uri uri-at-watson.ibm.com -=-=-=-=-=-=- {Disclaimer}
Vendor Sponsored Short Courses at the UCF/Cirent Materials Characterization Facility
1) Specimen Preparation using the Tripod Polisher (3/12-3/13): sponsored by South Bay Technology, instructor: Ron Anderson, IBM.
2) Specimen Preparation using Focused Ion Beam Milling (3/18-3/19): sponsored by FEI/Philips, instructors: Lucille Giannuzzi, UCF and Fred Stevie, Cirent Semiconductor.
COST $750/person for each course or $1200 for both courses
Registration Fee covers all Program Materials and breakfast and lunch on both days
to register see: http://pegasus.cc.ucf.edu/~ampac
or contact:
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
If someone is going to make photocopies, please put me down for a set too, especially for the otholux but any of them would be welcome for the archive. Ed Sharpe
} } } } Dose anybody know where to purchase and/or have, a Service Manual and } Operator Manual for a Leitz Ortholux and a Labolux? } } Originals and or photo copies? } } Thank You } Joseph Passero } jp-at-spacelab.net }
To Randy Tindall: I assume you meant LKB Ultratome III...Try "NJWS-at-aol.com". Norm Woodside deals with used LKB equipment. Bob Santoianni Emory University Hospital Atlanta, GA robert_santoianni-at-emory.org
Topcon/Akashi 002B High-Res TEM for sale. Complete working machine, includes two holders, a double tilt and multi. Air table and Gatan image intensifier system, two complete wehnelts.
Has been under maintainence contract and fully serviced.
Price Negotiable
Contact Mr. Mike Frongillo (617) 253-5092 frong-at-mit.edu
Dr. David C. Bell Room 13-1818 E-mail: dcb-at-MIT.EDU Center for Mat. Sci. and Eng. PH: (617) 253-3317 Massachusetts Institute of Technology FAX: (617) 258-6478 77 Massachusetts Ave, Cambridge, MA 02139
This request comes from a co-worker, who is looking for a software program to plot the scatter of experimental orientation relationship data on a stereographic projection. Please respond to him directly (he is not on the listserver) at mangan-at-anvil.nrl.navy.mil. Thank you. - Richard Fonda
We have generated a considerable amount of data on orientation relationships between two phases using the EBSD/OIM technique on an SEM. I was hoping to plot this up on a stereographic projection, but I have up to 100 data points. I'd like to be able to choose one phase as the reference lattice and then plot three directions in the other phase that correspond to a given orientation relationship between the two. And I'd like to see how far off my experimental data is from the ideal orientation relationship as I define it. Is anyone aware of any software package that could do this for this many data points?
Thanks for your help-
Mike
_____________________________________________________________ Michael A. Mangan Naval Research Laboratory Code 6324 Washington, DC 20375 phone: (202)767-2318 fax: (202)767-2623 _____________________________________________________________
The common American device, originally used for mixing Ag and Hg for dentists, is called a Wig-L-Bug, and is available from many suppliers of infrared sampling devices, e.g.,
Pike Technologies Madison, WI 608-274-2721
There are other devices as well. If the first doesn't work out, mail me directly.
Leonard Corwin Research Chemist Fort Dodge Animal Health Princeton, NJ 08543-0400
Dear Henrik, I have only had success grinding and polishing diamond drill bits with diamond-embedded, brass polishing wheels of various grit sizes. I usually use 45 micron, 20 micron and 6 micron wheels to make a surface suitable for SEM and EDX. You wrote: } Dear All, } } In our lab we need procedure (cutting, grinding and polishing) of } SEM sample with diamond particles in metal matrix. Any suggestions?. } } Henrik } } -- } SEM-EDS Laboratory } Metal Ravne } Slovenia } http://www2.arnes.si/guest/sgszmera1/index.html } Regards, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
} } Dear all } } can anyone tell me if people still use BSA
ref...........
Valentine, R.C. (1961) Adv. Virus Res. 8, 287
or bacitracin
ref..............
Gregory. DW and Pirie, BJS, "Wetting Agents for electron microscopy of biological specimens" Proc. 5th European Congress on EM, 1972 (Manchester) p 234
as 'wetting agents'
yes. we still use bacitracin!
***************************************************** Mel Dickson, Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
------------------------------------------------------. } } } Dear Listmembers, } Does anybody use or have used ruthenium tetroxide as a fixative for } biological material? Any references? } Thanks in advance.
Original reference.... Luft JH, 1971 Ruthenium red & violet II ..... Anatomical Record 171 p 369
one of ours.....
CD Garland et al The preservation of surface-associated micro-organisms prepared for SEM...
J. Microscopy 116 pp 227-242
***************************************************** Mel Dickson, Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
Can anyone point me to a source of 3mm diameter carbon rods which are likely to be identical with those from my old (} 12 years) box. They were "National" Spectroscopic electrodes, made by Union Carbide Corp Carbon Products Division, 270 Park Av, NY, NY, and were "Carbon 0.12 x 12" L113SP". I have some bought recently from an independent supplier, which are noticeably softer, but they seem to have to get hotter in order to vaporise, so I would like to obtain some as above.
thanks
Ritchie
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Ritchie Sims wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } Hi } } Can anyone point me to a source of 3mm diameter carbon rods which are } likely to be identical with those from my old (} 12 years) box. } They were "National" Spectroscopic electrodes, made by Union Carbide } Corp Carbon Products Division, 270 Park Av, NY, NY, and were "Carbon } 0.12 x 12" L113SP". } I have some bought recently from an independent supplier, which are } noticeably softer, but they seem to have to get hotter in order to } vaporise, so I would like to obtain some as above. } } thanks } } Ritchie } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand
Mr Sims,
Most of the EM supply houses (in the United States at least) sell graphite rods as carbon rods. From your description, we believe that you got graphite this time. We at Ladd sell pure carbon rods. If you are interested we could quote you direct.
Deb Sicard Sales Mgr.
Disclaimer: Ladd Research is a vendor that sells carbon rods. -- LADD RESEARCH 13 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
How do TEM users store spare (i.e. not in use) specimen holders? We have four holders and only one in use at any one time - how to keep the other three 'clean' but readily accessible? The manufacturers' (JEOL) boxes are good but not air-tight and are too large (about 48cm long) for any dessicator I can find in the usual catalogues - Fisher, Jencons, Agar etc.
TIA
Alan Walker
***************************************************************************** Alan Walker Dept of Electronic and Electrical Engineering University of Sheffield Mappin Street Sheffield S1 3JD United Kingdom Phone: +44-(0)114-2225365 (direct) +44-(0)114-2222000 (switchboard)
What is the best way to store spare (as in, not in use right now) specimen holders? We have four holders, so three are left 'gathering dust' at any one time. The manufacturers' (JEOL) boxes are good but not air-tight, and too long for any dessicator cabinet I can find in UK catalogues.
TIA
Alan Walker
***************************************************************************** Alan Walker Dept of Electronic and Electrical Engineering University of Sheffield Mappin Street Sheffield S1 3JD United Kingdom Phone: +44-(0)114-2225365 (direct) +44-(0)114-2222000 (switchboard)
We have a Polaron E5100 sputter coater that recently started blowing the high voltage fuse. When I switch to "Set HT" and slowly increase the sputtering current, the current reading doesn't get above ~1 or 2 mA before the Buss GDC, 400 mA, 250 V fuse blows. Has anyone witnessed this before? Any ideas? A web search for Polaron revealed nothing. Does anyone know if they are still in business?
TIA
Bob
Dr. Robert R. Wise Department of Biology and Microbiology University of Wisconsin-Oshkosh Oshkosh, WI 54901
(920) 424-3404 tel (920) 424-1101 fax wise-at-uwosh.edu www.uwosh.edu/departments/biology/wise/wise.html
Tim Wakefield ----- / 101 Cary Hall / | \ / Auburn University, AL / --|-- \/ 36849 \ | /\ 334-844-3908 \ | / \ ----- \
On Sun, 14 Feb 1999 katie2468-at-netscape.net-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } LIVE HOT PHONE SEX! } NO CREDIT CARD NEEDED! } NO 1-900 FEES! } Just a regular long distance call! } CALL NOW!!! 1-473-473-4917 } } } } } } } } } } } } } } } } } -To be removed please reply with "remove" in the subject line and } you will not receive any more messages from this email address. } } } } } } } } }
The Department of Anatomy and Physiology at Kansas State University has a 1977 Phillips Electron Microscope, Model EM400 for sale. This scope was purchased in 1977 by Oral Roberts University for $142,404.00. It was sold to Kansas State University in 1990 for $25,000.00. While here at Kansas State, this microscope has been well cared for and has been under a constant maintenance agreement with FEI. The faculty member who has been using this scope is retiring and no other faculty member has use for it. It is in excellent working condition. Please contact: Dani Goodband 785-532-4538 goodband-at-vet.ksu.edu
I don't know how many microscopists use Photoshop, but the following is text I submitted to the "photoshop list". If you believe in maintaining the integrity of image files, I think you ought to be made aware of Photoshop 5 defaults: (1) to enable ICC profiles and (2) the default color space (sRGB) gamma of 2.2.
~~~~~~~~
I've just installed PS 5 for Windows and have everything calibrated. But I think I'm going to object to the gamma for sRGB and bruceRGB being set to 2.2. I'm objecting for the sake of all my legacy files which were created with monitor compensation near gamma=1.6 which is approximately where 50%blackpoint and 50% whitepoint equals 50% gray ... which I believe (correct me if I'm wrong) is the basis for monitor compensation if you use "Adobe Gamma". A gamma = 2.2 has not been arbitrarily selected for sRGB and bruceRGB, reasons being "most displays", wwweb graphics, and the shadow tonal range. As a test I created with PS4 a RGB image with a flat histogram for all channels, and imported it into PS5 "from" a profile in accordance with my previous compensation and "into" bruceRGB color space. Upon viewing the bruceRGB profiled image it is accurate, HOWEVER if I now examine the histogram it is obvious the translation had done a major number on the pixel values.
I only mention this for the sake of legacy files ... that is, I can understand the profile reasoning from creation to final product. However, I don't know what to suggest. I understand the choice of gamma=2.2, but I believe it assumes most displays will never be compensated and that wwweb graphics will never display in accordance with ICC profiles. If that proves untrue, then the pros will in the future probably suggest a smaller gamma (1.8) and in the mean time we'll have created a lot of fine work profiled for a 2.2 display ... and from what I saw happen to my histogram, I suggest we put out work thru as few profiles as possible.
Can I ask how many of you really do work with sRGB=2.2??
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
Maggy Piranian wrote: } } Deb, I saw your response to Ritchie. What is the difference between carbon } and graphite? What are their different virtues and vices? } Maggy Piranian } ***************************************************************** } } Maggy Piranian } Electron Microprobe & X-Ray Diffraction Labs } Dept. of Earth Sciences } Memorial University of Newfoundland } St. John's, Newfoundland Phone (709) 737 8244 } A1B 3X5 Fax (709) 737 2589 } maggy-at-sparky2.esd.mun.ca } *****************************************************************Ladd offers both carbon and graphite but the majority of our customers ask for pure carbon, so that is our standard. Our own lab people feel that carbon provides a superior coating for our substrates.
Both our carbon and graphite are spectroscopic grades (extremely low impurity level). Pros/cons of both are:
Carbon - Harder to cut Needs less amps to heat Preferred by most of our users
Graphite - Softer, easier to cut Needs more amps (heat) to evaporate.
Disclaimer: Ladd Research is a vendor of microscopy supplies.
John Arnott -- LADD RESEARCH 13 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
In a message dated 99-02-15 12:45:15 EST, wise-at-vaxa.cis.uwosh.edu writes:
{ { We have a Polaron E5100 sputter coater that recently started blowing the high voltage fuse. When I switch to "Set HT" and slowly increase the sputtering current, the current reading doesn't get above ~1 or 2 mA before the Buss GDC, 400 mA, 250 V fuse blows. Has anyone witnessed this before? Any ideas? A web search for Polaron revealed nothing. Does anyone know if they are still in business?
TIA
Bob } }
Hi Bob,
Polaron vacuum and coating devices are sold by Energy Beam Sciences in Agawam, MA. You can reach them as follows:
Energy Beam Sciences, Inc. P.O. Box 468 11 Bowles Road Agawam, MA 01001 Tel. (800) 992-9037 Fax (413) 789-2786 www.ebsciences.com
I'm certain they can help you solve your problem.
Cheers,
Bob ****************************** Robert (Bob) Chiovetti, Ph.D. President Microimaging Technologies, Inc. Tucson, Arizona USA Tel./Fax (520) 546-4986 rchiovetti-at-aol.com Manufacturers' Representatives Systems Integrators Analog & Digital Imaging Systems Clinical & Research Microscopy Cytology/Histology/Pathology/EM *******************************
Maggy Piranian wrote: } } Deb, I saw your response to Ritchie. What is the difference between carbon } and graphite? What are their different virtues and vices? } Maggy Piranian}
Sorry, a few lines were missing from our first response:
Ladd offers both carbon and graphite, but the majority of our customers ask for pure carbon so that is our standard. Our own lab people feel that carbon provides a superior coating for our substrates.
Both our carbon and graphite are spectroscopic (extremely low impurity level). Pros/cons:
Carbon - harder to cut needs less amps to heat preferred by most customers
Graphite - softer, easier to cut needs more amps (heat) to evaporate.
disclaimer: Ladd Research is a vendor of microscopy supplies
John Arnott -- LADD RESEARCH 13 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
We have used a 5100 here for many years. I think it has acted up similarly. After having problems reaching vacuum, I pulled it apart and gave it a good cleaning. There was plenty of gold all over the place. I think some of it was even in places where it may have caused a short to the frame. I got over 15 g of gold scraping off what had built up over the years.
Warren
At 11:15 AM 2/15/99 -0600, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Just a short heads up. I'll be off-line for a day or so.While I don't expect problems, Murphy says something will go wrong on the List. To minimize problems. I will put Subscriptions and Unscriptions on hold until I reconnect. Please be patient until I get back on the Net.
BTW, yes I did see the XXX mail, and I have added that address to the SPAM filter. However, please remember this is a public posting site and junk mail will occassional get through. The filter, theoretically, permits this only once from a given address, but SPAMer's generally post from different (and faked) addresses all the time. Most of the junk mail is caught, but there will be some that gets through.
Union Carbide sold its line of spectroscopic electrodes to Ultra Carbon in Bay City, Michigan about 15-20 years ago. Shortly thereafter, Union Carbide was fragmented, and UCAR Carbon Company is the remnants of the Union Carbide Carbon Products Division.
The research laboratories at UCAR have probably the only remaining stock of genuine Union Carbide spectroscopic electrodes. We do have a few of the grade and size you want remaining. If you are interested, please contact me.
Helen Mayer UCAR Carbon Company 12900 Snow Road Parma, OH 44130 216-676-2373 FAX 216-676-2276 helen.mayer-at-ucar.com
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi
Can anyone point me to a source of 3mm diameter carbon rods which are likely to be identical with those from my old (} 12 years) box. They were "National" Spectroscopic electrodes, made by Union Carbide Corp Carbon Products Division, 270 Park Av, NY, NY, and were "Carbon 0.12 x 12" L113SP". I have some bought recently from an independent supplier, which are noticeably softer, but they seem to have to get hotter in order to vaporise, so I would like to obtain some as above.
thanks
Ritchie
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
A student in our lab would like to do TEM on Drosophila eggs to look specifically at the micropyle. Our initial fixation was based on a paper on amphibian eggs. We fixed in 3% glutaraldehyde, 2% formaldehyde, 1% acrolein and 2.5% DMSO in 0.1 M cacodylate buffer overnight at room temperature, followed by 2% osmium, 0.2 M sucrose in 0.1M cacodylate buffer, overnight at room temperature. Eggs were then dehydrated in acetone and embedded in EPON.
We observed significant seperation (complete detachment actually) of the chorion from the egg. In fact the chorion appeared to be almost twice the size of the egg. Any advice on why this happened or a better way to fix would be greatly appreciated.
Thanks in advance, Michelle
#################################################### Michelle Peiffer Electron Microscope Facility for the Life Sciences The Biotechnology Institute for Research and Education 1 South Frear Lab University Park, PA 16802 814-865-0212 email:mlk101-at-psu.edu ####################################################
As an accessory to our plasma cleaner we also offer Vacuum Storage Containers. Typically, these are used for short term storage when transferring from the plasma cleaner to the microscope. However, these same storage containers can be fitted with the appropriate valves to evacuate the container, remove water vapor, back fill with dry N2, and store over longer periods of time and be used separate from the plasma cleaner.
We can customize these storage stations to enable you to store multiple holders - even from different manufacturers - in one manifold with individual valves etc. I would be pleased to discuss this system with yo= u further. Please contact me off-line for additional information.
DISCLAIMER: South Bay Technology manufacturers systems for specimen cleaning and storage and therefore I have a vested interest in promoting their use.
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by "A.Walker" } ------------------------------------------------------------------------=
The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
Hi,
How do TEM users store spare (i.e. not in use) specimen holders? We have four holders and only one in use at any one time - how to keep the other three 'clean' but readily accessible? The manufacturers' (JEOL) boxes are good but not air-tight and are too large (about 48cm long) for any dessicator I can find in the usual catalogues - Fisher, Jencons, Agar etc.
I am looking for a commercial source of a good GABA-A beta chain subunit antibody. The one we are using is giving non-specific labeling. Thanking you in advance, Lilith ------------------------------------------------------------------ Lilith Ohannessian-Barry National Research Council Institute of Biological Sciences CANADA Tel;613-993-6460 Fax;613-941-4475 e-mail; lilith.barry-at-nrc.ca
There was an excellent article on selecting an RGB working space in the Adobe Photoshop magazine they send free to register users. It was the autumn 1998 issue on p.51- 56. it is probably on their web site in an archive or they will fax it to you. The author concluded that sRGB was the worst possible choice of many. The author thought ColorMatch RGB was a better gamut space to work with. I just read the article this weekend (it is very dense - or maybe i am the dense one but once you get it, it seems very useful. Tom
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211 (573)-882-4712 (voice) (573)-882-0123 (fax)
Can anyone advise a fax number, an e-mail address, or a website for Agar Aids, UK? Their clocks are 13 hours out of synch with mine, difficult to phone them.
thanks and thanks also to the responders re carbon rods, very interesting to realise the difference between "carbon" rods and "graphite" ones.
cheers
rtch
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Your comment re: "how many microscopists use Photoshop" is interesting and has a major impact on the scientific quality of microscopy. MME just conducted research at the Cell Biology meeting in December and asked that question. Fifty-four percent of our sample of nearly 500 participants answered this question and 86 % (46% of the total survey population) indicated that they use Photoshop! (And you can quote us on that).
A reminder that this software package was initially designed for a graphics audience which does not need to be concerned with the validity of data carried by each pixel. Clearly, it is important that any issues which affect the scientific content of these images be carefully calibrated and controlled.
Hope this insight is helpful.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 09:48 AM 2/15/99 -0800, shAf wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Subject: SEM "hole and pit" Analysis Trying to update my current software with crrent "automatic or semi-automatic" software and a manual "digitizing Pen" techniques. Because of the edge contrast in SEM, I've use edge detection techniques such as watershed and an old commercial software called "WICS" (which I can't find). Any suggestions? To avoid tieing up the server please respond directly after the first few responses. Thank you. Jeff Day/ "CD" WA5EKH-at-JUNO.COM
___________________________________________________________________ You don't need to buy Internet access to use free Internet e-mail. Get completely free e-mail from Juno at http://www.juno.com/getjuno.html or call Juno at (800) 654-JUNO [654-5866]
In a message dated 2/16/99 12:07:58 PM, mme-at-map.com writes:
} Your comment re: "how many microscopists use Photoshop" is interesting } and has a major impact on the scientific quality of microscopy. MME just } conducted research at the Cell Biology meeting in December and asked that } question. Fifty-four percent of our sample of nearly 500 participants } answered this question and 86 % (46% of the total survey population) } indicated that they use Photoshop! (And you can quote us on that).
Thanks, Barbara. Although the original point of this thread was the choice of an appropriate color space for imaging, the more general point about Photoshop is one that I believe in as well. That is why we wrote The Image Processing Tool Kit to run with it. This provides the functionality of dedicated image analysis packages costing many thousands of dollars for a few hundred (sorry about the commercial plug on this list, but I think my point here is relevant) by taking advantage of Photoshop as the host. This program supports acquisition with just about every device out there, offers a uniform user interface on Macs and PCs, takes care of storage, printing, virtual memory, etc. etc. (all the system stuff), and (with rev 5) offers a modest amount of automation. It is easily learned and relatively inexpensive. It is also a wonderfully straightforward package for learning about scientific image analysis, with an extensive on-disk tutorial and examples (mostly images from The Image Processing Handbook). Apparently the users agree, because we've sold several thousand copies of the tool kit so far. (http://members.aol.com/ImagProcTK)
Hi Christopher; I have had very good luck staining starch with PAS, followed by counterstain with light green. the starch stains a bright fuschia, and the protein varying intensities of green (depending on type of protein and relative amounts). This was done on GMA sections, btw. If you need more information, feel free to contact me.k cheers shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada Eastern Cereal and Oilseed Research Centre 2068 K.W. Neatby Bldg Central Experimental Farm Ottawa, Ontario Canada K1A 0C6 email: millers-at-em.agr.ca phone: 613-759-1760 fax: 613-759-1701 ! !
Dear Ritchie, I found there was a difference between carbon rods and graphite rods. I prefer the carbon. You may have been supplied the graphite, which, as you say, needs to be heated higher to vaporize. I went back to Carbone of America, Ultra Carbon Div. for spectrographic grade pressed carbon rods. The latest address I have is: ultra carbon 900 Harrison St. Bay City, MI 48708-8244 USA
Good luck, Regards, Mary
At 04:13 PM 15/02/99 GMT+1200, you wrote: } } } Hi } } } } Can anyone point me to a source of 3mm diameter carbon rods which are } } likely to be identical with those from my old (} 12 years) box. } } They were "National" Spectroscopic electrodes, made by Union Carbide } } Corp Carbon Products Division, 270 Park Av, NY, NY, and were "Carbon } } 0.12 x 12" L113SP". } } I have some bought recently from an independent supplier, which are } } noticeably softer, but they seem to have to get hotter in order to } } vaporise, so I would like to obtain some as above. } } } } thanks } } } } Ritchie } } Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
Dear Ritchie, The listing in the Kaker.com vendor database shows: Agar Scientific Ltd. 66a Cambridge Road CM24 8DA Stansted UK Tel: +44 1279 81 35 19 Fax: +44 1279 81 51 06 Grids, calibration specimen, tweezers, small tools, light microscope accessories, optical aids, steroscopy, specimen preparation equipment, biological specimen preparation, chemicals, materials science specimen preparation, image storage and analys is, photo materials and equipment, cleaninig materials, safety equipment, publications, starters kits. I was unable to find a Web site listing. At leaast fax will help with the time difference. You wrote:
} Can anyone advise a fax number, an e-mail address, or a website for } Agar Aids, UK? } Their clocks are 13 hours out of synch with mine, difficult to phone } them. } } thanks and thanks also to the responders re carbon rods, very interesting } to realise the difference between "carbon" rods and "graphite" ones. } } cheers } } rtch } Regards, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
Tom writes ... } } } There was an excellent article on selecting an RGB working } space in the Adobe Photoshop magazine ... The author concluded } that sRGB was the worst possible choice of many. ...
That article was written by Bruce Fraser. I haven't read the article, but I'm reading his and Blatner's "Real World Photoshop 5" which I highly reccommend for unsderstanding the underpinnings of PS's handling of color management. Bruce has created "bruceRGB" which he describes as "social-democratic alternative ..." where sRGB and Color match RGB" are the two ends of a common spectrum. However, where Bruce deviates radically from Color Match is his choice of gamma=2.2 for the working space gamma (cmRGB gamma=1.8). I understand his point of view if your display gamma is indeed 2.2 .. but if you read (for example) a spacial distribution of an elemental composition into this color space (where the gamma is considered unity), the PS conversion (bruceRGB or sRGB) will indeed do a number on the image map's histogram. I do understand Photoshop is used within our community primarily for presentation and not really as a data tool ... I only mention this to those who may be considering the PS5 upgrade and as a word of caution.
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I agree. I purchased a copy of the toolkit and have found it a very handy addition to Photoshop. You get boolean math, background adjustment, fft stuff, thresholding, auto-counting capabilities and much more. It appears to me to do just about everything that my (orders of magnitude) more expensive image processing software does with the exception of macro writing capabilities. I've been using it lots for semi-automatic counting. I add a grid to my acquired image and then use the mouse to mark intersections with the grid. The program will count the number of marks you put on with your mouse. If you are using Photoshop it is a worthwhile addition to your lab software even if you have a full blown image processing package. If you don't have a full-blown image processing program (and can't afford one) then it would be EXTREMELY helpful. John also has several useful books about image processing. All very practical, knowledgable and useful in my opinion.....
Robin Griffin - who has no financial interest in John's stuff!
-----Original Message----- } From: "DrJohnRuss-at-aol.com"-at-Sparc5.Microscopy.Com [mailto:"DrJohnRuss-at-aol.com"-at-Sparc5.Microscopy.Com] Sent: Tuesday, February 16, 1999 7:50 AM To: mme-at-map.com; Microscopy-at-Sparc5.Microscopy.Com
In a message dated 2/16/99 12:07:58 PM, mme-at-map.com writes:
} Your comment re: "how many microscopists use Photoshop" is interesting } and has a major impact on the scientific quality of microscopy. MME just } conducted research at the Cell Biology meeting in December and asked that } question. Fifty-four percent of our sample of nearly 500 participants } answered this question and 86 % (46% of the total survey population) } indicated that they use Photoshop! (And you can quote us on that).
Thanks, Barbara. Although the original point of this thread was the choice of an appropriate color space for imaging, the more general point about Photoshop is one that I believe in as well. That is why we wrote The Image Processing Tool Kit to run with it. This provides the functionality of dedicated image analysis packages costing many thousands of dollars for a few hundred (sorry about the commercial plug on this list, but I think my point here is relevant) by taking advantage of Photoshop as the host. This program supports acquisition with just about every device out there, offers a uniform user interface on Macs and PCs, takes care of storage, printing, virtual memory, etc. etc. (all the system stuff), and (with rev 5) offers a modest amount of automation. It is easily learned and relatively inexpensive. It is also a wonderfully straightforward package for learning about scientific image analysis, with an extensive on-disk tutorial and examples (mostly images from The Image Processing Handbook). Apparently the users agree, because we've sold several thousand copies of the tool kit so far. (http://members.aol.com/ImagProcTK)
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
---------- Forwarded message ----------
Tuesday, February 16, 1999
InvestingNow.com Wrote
http://www.InvestingNow.com
At 8 AM stocks appear to be set for a big move this morning. The S&P 500 futures were up 7.2, more than 11 above fair value and cueing the market for a good jump upward at the open. At 8:10 a.m. EST, the 30-year Treasury bond was up 28/32 to 98 10/32, dropping the yield to 5.36%.
Japan's Nikkei added 177.92 to 14,232.64. Hong Kong's stock market was closed for the Chinese New Year holiday. Europe's major averages were all higher. In Frankfurt, the Dax was up 15.24 to 4894.79. In Paris, the CAC was up 10.33 to 4075.52. In London, the FTSE was up 93.2, or 1.5%, to 6116.4.
Dell (DELL:NYSE) and Hewlett-Packard (HWP:NYSE) will report earnings after the close. American Airlines expects nearly all its flight schedule to be back to normal today. American is a unit of AMR (AMR:NYSE).
Volvo (VOLVY:Nasdaq ADR) of Sweden is considering a takeover of Navistar (NAV:NYSE), the U.S. truck and engine manufacturer, in a move that could more than double Volvo's share of the North American truck market, the Financial Times reported. Earlier this month, Volvo agreed to sell its car division to Ford (F:NYSE). Chicago-based Navistar has a market capitalization of $2.3 billion, meaning that a buyer would probably have to pay at least $3 billion to take full control of the company, the newspaper said. If Volvo, however, were only to buy Navistar's heavy truck operations, the price could be substantially lower, the newspaper said.
The Dow Jones Industrial Average (DJIA) should maintain its trading range of 9,000 to 9,100 on the bottom end and 9,375 to 9,500 on the top end. These markets have been fantastic for trading the last week. As can be seen from our calls, for short-term calls they have been predictable. The wild card continues to be the technology sector. The nervousness Friday concerning DELL will continue today as we await the earning announcement this afternoon. Overall we are positive on the DJIA and broad market, and continue to dislike technology stocks. For the long term we remain BULLISH.
Did you receive our e-mail execution to cover our short in Microsoft on Friday? We covered this short in MSFT at $158 at the close on Friday afternoon. This short was established on February 3, 1999 at $168. Be sure to check out our trading suggestions and portfolio recommendations regularly. And get on our mailing list!
At 8 AM stocks appear to be set for a big move this morning. The S&P 500 futures were up 7.2, more than 11 above fair value and cueing the market for a good jump upward at the open. At 8:10 a.m. EST, the 30-year Treasury bond was up 28/32 to 98 10/32, dropping the yield to 5.36%.
Japan's Nikkei added 177.92 to 14,232.64. Hong Kong's stock market was closed for the Chinese New Year holiday. Europe's major averages were all higher. In Frankfurt, the Dax was up 15.24 to 4894.79. In Paris, the CAC was up 10.33 to 4075.52. In London, the FTSE was up 93.2, or 1.5%, to 6116.4.
Dell (DELL:NYSE) and Hewlett-Packard (HWP:NYSE) will report earnings after the close. American Airlines expects nearly all its flight schedule to be back to normal today. American is a unit of AMR (AMR:NYSE).
Volvo (VOLVY:Nasdaq ADR) of Sweden is considering a takeover of Navistar (NAV:NYSE), the U.S. truck and engine manufacturer, in a move that could more than double Volvo's share of the North American truck market, the Financial Times reported. Earlier this month, Volvo agreed to sell its car division to Ford (F:NYSE). Chicago-based Navistar has a market capitalization of $2.3 billion, meaning that a buyer would probably have to pay at least $3 billion to take full control of the company, the newspaper said. If Volvo, however, were only to buy Navistar's heavy truck operations, the price could be substantially lower, the newspaper said.
The Dow Jones Industrial Average (DJIA) should maintain its trading range of 9,000 to 9,100 on the bottom end and 9,375 to 9,500 on the top end. These markets have been fantastic for trading the last week. As can be seen from our calls, for short-term calls they have been predictable. The wild card continues to be the technology sector. The nervousness Friday concerning DELL will continue today as we await the earning announcement this afternoon. Overall we are positive on the DJIA and broad market, and continue to dislike technology stocks. For the long term we remain BULLISH.
Did you receive our e-mail execution to cover our short in Microsoft on Friday? We covered this short in MSFT at $158 at the close on Friday afternoon. This short was established on February 3, 1999 at $168. Be sure to check out our trading suggestions and portfolio recommendations regularly. And get on our mailing list!
I have an instruction booklet(no service manual) for the Ortholux, as well as booklets for attachments called Ultropak and Interference Contrast Device T. Someone give me an address to send copies to.
Julie Gross UCONN Health Center jgross-at-neuron.uchc.edu
Another plus with respect to using Image Processing Toolkit Version 2.5 is that John has included his text on Stereology on the CD-ROM. -Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
---------- } From: "DrJohnRuss-at-aol.com"-at-Sparc5.Microscopy.Com To: mme-at-map.com; Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
In a message dated 2/16/99 12:07:58 PM, mme-at-map.com writes:
} Your comment re: "how many microscopists use Photoshop" is interesting } and has a major impact on the scientific quality of microscopy. MME just } conducted research at the Cell Biology meeting in December and asked that } question. Fifty-four percent of our sample of nearly 500 participants } answered this question and 86 % (46% of the total survey population) } indicated that they use Photoshop! (And you can quote us on that).
Thanks, Barbara. Although the original point of this thread was the choice of an appropriate color space for imaging, the more general point about Photoshop is one that I believe in as well. That is why we wrote The Image Processing Tool Kit to run with it. This provides the functionality of dedicated image analysis packages costing many thousands of dollars for a few hundred (sorry about the commercial plug on this list, but I think my point here is relevant) by taking advantage of Photoshop as the host. This program supports acquisition with just about every device out there, offers a uniform user interface on Macs and PCs, takes care of storage, printing, virtual memory, etc. etc. (all the system stuff), and (with rev 5) offers a modest amount of automation. It is easily learned and relatively inexpensive. It is also a wonderfully straightforward package for learning about scientific image analysis, with an extensive on-disk tutorial and examples (mostly images from The Image Processing Handbook). Apparently the users agree, because we've sold several thousand copies of the tool kit so far. (http://members.aol.com/ImagProcTK)
At 8 AM stocks appear to be set for a big move this morning. The S&P 500 futures were up 7.2, more than 11 above fair value and cueing the market for a good jump upward at the open. At 8:10 a.m. EST, the 30-year Treasury bond was up 28/32 to 98 10/32, dropping the yield to 5.36%.
Japan's Nikkei added 177.92 to 14,232.64. Hong Kong's stock market was closed for the Chinese New Year holiday. Europe's major averages were all higher. In Frankfurt, the Dax was up 15.24 to 4894.79. In Paris, the CAC was up 10.33 to 4075.52. In London, the FTSE was up 93.2, or 1.5%, to 6116.4.
Dell (DELL:NYSE) and Hewlett-Packard (HWP:NYSE) will report earnings after the close. American Airlines expects nearly all its flight schedule to be back to normal today. American is a unit of AMR (AMR:NYSE).
Volvo (VOLVY:Nasdaq ADR) of Sweden is considering a takeover of Navistar (NAV:NYSE), the U.S. truck and engine manufacturer, in a move that could more than double Volvo's share of the North American truck market, the Financial Times reported. Earlier this month, Volvo agreed to sell its car division to Ford (F:NYSE). Chicago-based Navistar has a market capitalization of $2.3 billion, meaning that a buyer would probably have to pay at least $3 billion to take full control of the company, the newspaper said. If Volvo, however, were only to buy Navistar's heavy truck operations, the price could be substantially lower, the newspaper said.
The Dow Jones Industrial Average (DJIA) should maintain its trading range of 9,000 to 9,100 on the bottom end and 9,375 to 9,500 on the top end. These markets have been fantastic for trading the last week. As can be seen from our calls, for short-term calls they have been predictable. The wild card continues to be the technology sector. The nervousness Friday concerning DELL will continue today as we await the earning announcement this afternoon. Overall we are positive on the DJIA and broad market, and continue to dislike technology stocks. For the long term we remain BULLISH.
Did you receive our e-mail execution to cover our short in Microsoft on Friday? We covered this short in MSFT at $158 at the close on Friday afternoon. This short was established on February 3, 1999 at $168. Be sure to check out our trading suggestions and portfolio recommendations regularly. And get on our mailing list!
} What are operating temperatures of the two Diffusion pumps in a JEOL } 100-CX, and can I replace the oil with Santovac 5. } I do not know ofhand what the operating temperature is but it is using a 200V 600W heater. We have been running the 100S TEM and 100C TEM as well as JSM 840 Scanner on Santovac 5 without anny problems.
Hope this is some help.
Mr. S H Coetzee Electron Microscope Unit Private bag X3 Wits Johannesburg 2050 Tell: +27 11 716 2419 Fax : +27 11 339 3407 E-mail stephan-at-gecko.biol.wits.ac.za
To Laurence Marks and Amy Yarbrough, There is an excellent history of the Electron Microscope, "EMSA and Its People--the First Fifty Years" by Sterling P. Newberry. It was published in 1992 by EMSA (new MSA).
A group here in the medical school needs to do some immunoEM and will require cryoultramicrotomy. Are there any labs in the Boston area which have this capability and would make it available perhaps on a fee for service basis? Thanks. Don Gantz Boston Univ Med School gantz-at-med-biophd.bu.edu 617-638-4017
A colleague here asked me whether I knew a simple stain for bacteria that would selectively demonstrate the presence or absence of lipid. The idea would be to stain E. coli before or after lipid extraction, and see in a light microscope whether the lipid had been removed.
I know nothing about microbial stains. Any thoughts on whether this is possible or how to go about this experiment?
Gary Radice 804-289-8107 Department of Biology 804-289-8233 (FAX) University of Richmond gradice-at-richmond.edu Richmond VA 23173
As a supplier of items for microscopy we may be able to help you with your storage problem. If our standard desiccator cabinet is too small we can, (at extra cost sadly), probably provide a custom unit. I will send details of our stock item.
All commercial disclaimers apply
Terry Cooper TAAB Laboratories Equipment Ltd 3 Minerva House, Calleva Park Aldermaston, Berks, RG7 8NA, UK
Dr. E. McCain at Muhlenberg College, Allentown, PA, is looking for an aperture drive for a Carl Zeiss Transmission Microscope EM109. If anyone out there can help her, please contact her directly at mccain-at-muhlenberg.edu. Thanks in advance. Pete Dondl
I recently attended a two day course on adhesion. The topic of the different types of cleaning came up and one that was mentioned was UV/ozone. I am familiar with that and I thought that someone had told me that they stored their TEM holders in a UV light box. The problem is that it takes two wavelengths of UV for this type of cleaning to work (One that creates the ozone and one that dissociates it to form nascent oxygen). Are there any systems out there that have ports for holders could be inserted in order for them to maintain their cleanliness while not being used? You would not want a system that the whole holder gets into because the UV might degrade the plastic parts and O-ring seals.
Just curious.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
This is about the Image Processing Tool Kit available from Dr. John Russ = and Chris Russ, which are plug ins for Photoshop and NIH Image.
Just wanted to add that not only is it a great addition for Photoshop and = NIH Image as everyone has already mentioned, it also includes a nice = tutorial that students can use to learn how to use the filters. It = further helps them develop their skills in using Photoshop. We use it = for part of our Digital Imaging Course. It really makes Photoshop a much = more powerful program than it already is.
I have no financial interest in the product, but I do love it.
Judy M.
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
__________________________________________________________________________= _____ } From: Walck. Scott D. on Tue, Feb 16, 1999 6:14 PM
Another plus with respect to using Image Processing Toolkit Version 2.5 = is that John has included his text on Stereology on the CD-ROM. -Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
---------- } From: "DrJohnRuss-at-aol.com"-at-Sparc5.Microscopy.Com To: mme-at-map.com; Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
In a message dated 2/16/99 12:07:58 PM, mme-at-map.com writes:
} Your comment re: "how many microscopists use Photoshop" is interesting } and has a major impact on the scientific quality of microscopy. MME = just } conducted research at the Cell Biology meeting in December and asked = that } question. Fifty-four percent of our sample of nearly 500 participants } answered this question and 86 % (46% of the total survey population) } indicated that they use Photoshop! (And you can quote us on that).
Thanks, Barbara. Although the original point of this thread was the = choice of an appropriate color space for imaging, the more general point about Photoshop is one that I believe in as well. That is why we wrote The Image = Processing Tool Kit to run with it. This provides the functionality of dedicated = image analysis packages costing many thousands of dollars for a few hundred = (sorry about the commercial plug on this list, but I think my point here is relevant) by taking advantage of Photoshop as the host. This program supports acquisition with just about every device out there, offers a uniform user interface on Macs and PCs, takes care of storage, printing, virtual = memory, etc. etc. (all the system stuff), and (with rev 5) offers a modest amount = of automation. It is easily learned and relatively inexpensive. It is also a wonderfully straightforward package for learning about scientific image analysis, with an extensive on-disk tutorial and examples (mostly images from The Image Processing Handbook). Apparently the users agree, because we've sold several thousand copies of the tool kit so far. (http://members.aol.com/ImagProcTK)
John Russ
------------------ RFC822 Header Follows ------------------ Received: by sjdccd.cc.ca.us with ADMIN;16 Feb 1999 18:14:18 -0800 Received: from Sparc5.Microscopy.Com ([206.69.208.10]) by ent2.sjdccd.cc.ca.us (Netscape Messaging Server 3.6) with SMTP id 296 for {jmurphy-at-sjdccd.cc.ca.us} ; Tue, 16 Feb 1999 18:13:57 -0800 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com = (8.6.11/8.6.11) id PAA22384 for dist-Microscopy; Tue, 16 Feb 1999 = 15:18:10 -0600 Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by = Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id PAA22370 for = "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 16 Feb 1999 15:17:39 = -0600 Received: from gateway.ppg.com (gateway.ppg.com [199.221.65.2]) by = Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id PAA22358 for = {microscopy-at-sparc5.microscopy.com} ; Tue, 16 Feb 1999 15:17:25 -0600 Received: by gateway.ppg.com id QAA25744 (SMTP Gateway for microscopy-at-sparc5.microscopy.com); Tue, 16 Feb 1999 16:33:07 -0500 Message-Id: {199902162133.QAA25744-at-gateway.ppg.com} Received: by gateway.ppg.com (Protected-side Proxy Mail Agent-1); Tue, 16 Feb 1999 16:33:07 -0500 } From: "Walck. Scott D." {walck-at-ppg.com} To: Micro {microscopy-at-Sparc5.Microscopy.Com}
I'm looking for a used unit in working condition. This 1600T has the "universal" stage....small round model. Need the detector and amplifier with BNC connector interface to signal selector switch.
I'm looking for a used unit in working condition. This 1600T has the "universal" stage....small round model. Need the detector and amplifier with BNC connector interface to signal selector switch.
This is about the Image Processing Tool Kit available from Dr. John Russ and Chris Russ, which are plug ins for Photoshop and NIH Image.
Just wanted to add that not only is it a great addition for Photoshop and NIH Image as everyone has already mentioned, it also includes a nice tutorial that students can use to learn how to use the filters. It further helps them develop their skills in using Photoshop. We use it for part of our Digital Imaging Course. It really makes Photoshop a much more powerful program than it already is.
I have no financial interest in the product, but I do love it.
Judy M.
Judy Murphy Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209/954-5284 FAX 209/954-5600 e-mail; jmurphy-at-sjdccd.cc.ca.us
_______________________________________________________________________________ } From: Walck. Scott D. on Tue, Feb 16, 1999 6:14 PM
Another plus with respect to using Image Processing Toolkit Version 2.5 is that John has included his text on Stereology on the CD-ROM. -Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
---------- } From: "DrJohnRuss-at-aol.com"-at-Sparc5.Microscopy.Com To: mme-at-map.com; Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
In a message dated 2/16/99 12:07:58 PM, mme-at-map.com writes:
} Your comment re: "how many microscopists use Photoshop" is interesting } and has a major impact on the scientific quality of microscopy. MME just } conducted research at the Cell Biology meeting in December and asked that } question. Fifty-four percent of our sample of nearly 500 participants } answered this question and 86 % (46% of the total survey population) } indicated that they use Photoshop! (And you can quote us on that).
Thanks, Barbara. Although the original point of this thread was the choice of an appropriate color space for imaging, the more general point about Photoshop is one that I believe in as well. That is why we wrote The Image Processing Tool Kit to run with it. This provides the functionality of dedicated image analysis packages costing many thousands of dollars for a few hundred (sorry about the commercial plug on this list, but I think my point here is relevant) by taking advantage of Photoshop as the host. This program supports acquisition with just about every device out there, offers a uniform user interface on Macs and PCs, takes care of storage, printing, virtual memory, etc. etc. (all the system stuff), and (with rev 5) offers a modest amount of automation. It is easily learned and relatively inexpensive. It is also a wonderfully straightforward package for learning about scientific image analysis, with an extensive on-disk tutorial and examples (mostly images from The Image Processing Handbook). Apparently the users agree, because we've sold several thousand copies of the tool kit so far. (http://members.aol.com/ImagProcTK)
John Russ
------------------ RFC822 Header Follows ------------------ Received: by sjdccd.cc.ca.us with ADMIN;16 Feb 1999 18:14:18 -0800 Received: from Sparc5.Microscopy.Com ([206.69.208.10]) by ent2.sjdccd.cc.ca.us (Netscape Messaging Server 3.6) with SMTP id 296 for {jmurphy-at-sjdccd.cc.ca.us} ; Tue, 16 Feb 1999 18:13:57 -0800 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id PAA22384 for dist-Microscopy; Tue, 16 Feb 1999 15:18:10 -0600 Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id PAA22370 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 16 Feb 1999 15:17:39 -0600 Received: from gateway.ppg.com (gateway.ppg.com [199.221.65.2]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id PAA22358 for {microscopy-at-sparc5.microscopy.com} ; Tue, 16 Feb 1999 15:17:25 -0600 Received: by gateway.ppg.com id QAA25744 (SMTP Gateway for microscopy-at-sparc5.microscopy.com); Tue, 16 Feb 1999 16:33:07 -0500 Message-Id: {199902162133.QAA25744-at-gateway.ppg.com} Received: by gateway.ppg.com (Protected-side Proxy Mail Agent-1); Tue, 16 Feb 1999 16:33:07 -0500 } From: "Walck. Scott D." {walck-at-ppg.com} To: Micro {microscopy-at-Sparc5.Microscopy.Com}
} From: Administrator_at_RLT013-at-ccmailgw.mcgawpark.baxter.com } X-SMTP: helo gaugler.com from administrator_at_rlt013-at-ccmailgw.mcgawpark.baxter.com server -at-server14.aitcom.net ip 208.234.0.15 } Date: Wed, 17 Feb 1999 20:41:46 -0600 } To: "Dr. Gary Gaugler" {gary-at-gaugler.com} } Subject: Message not deliverable } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Chih-Hung, You could put them in a grid box and hand carry them to avoid any rough handling. Another option is to use membrane boxes which are boxes with PE membranes stretched across the openings. When sandwiched together they hold the grid in place suspended between the two membranes. They are commercially available. Russ
-----Original Message----- } From: "chanch-at-ufl.edu"-at-sparc5.microscopy.com [mailto:"chanch-at-ufl.edu"-at-sparc5.microscopy.com] Sent: Wednesday, February 17, 1999 6:50 PM To: microscopy-at-sparc5.microscopy.com
Dear Professional friends,
I have to use a distant microscope. What's the best way to transport (from FL to CO) fragile TEM samples?
best regards,
chih-hung chang Dept. of Chemical Engineering University of Florida Gainesville,FL 32611
I use membrane boxes, one sample per box. I've used them with III-V, II-VI, and now glass samples. You can buy them from a variety of EM supply houses. I use the one inch wide white Post-it tape to label the samples. When I'm done with the sample, I reuse the box and tear off the label. They stack nicely and you can pack a bunch into small boxes that are easily transportable. They also make great pill boxes. -Scott Walck
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
---------- } From: "chanch-at-ufl.edu"-at-Sparc5.Microscopy.Com To: microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Dear Professional friends,
I have to use a distant microscope. What's the best way to transport (from FL to CO) fragile TEM samples?
best regards,
chih-hung chang Dept. of Chemical Engineering University of Florida Gainesville,FL 32611
Chih-Hung, Transporting specimens is routinely undertaken by many people, the best method depends on your specimens and what you are going to do with them. Are they very fragile, air sensitive, contaminate easily or do they change with time? They can be transported by hand or mail. They can be sealed in an inert, dry, gas in a grid box, stored in alcohol filled tubes or packed in velin tissue and filter paper in a small box. Undoubtably there are many other ways as well. If you have particular requirements for your specimens then let us know and we will see if we can help with your specific problem,
Good luck, Ron
=========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
I'm hoping that someone will be able to help me out with this as I am primarily a biological science person.
1- Is there someone in the RTP area that has an SEM with an environmental chamber?
2- Any suggestions on a plastic polymer that could poured onto a bunch of particals (eg: sand) which after polymerization could be fractured and the fractured surface examined by an SEM so that partical size and intrastitial space measurments could be made using image analysis?
3- To complicate matters, it would be nice to seperate (again via SEM) the organics from the minerals vissually. We need to see if the organic is chemically attached to the mineral or if it forms aggregates between them.
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress .. But I repeat myself.-Mark Twain**
I'm hoping that someone will be able to help me out with this as I am a biological science person (give me a cell any time).
1- Is there someone in the RTP area that has an SEM with an environmental chamber?
2- Any suggestions on a plastic polymer that could poured onto a bunch of particals (eg: sand) which after polymerization could be fractured and the fractured surface examined by an SEM so that partical size and intrastitial space measurments could be made using image analysis?
3- To complicate matters, it would be nice to seperate (again via SEM) the organics from the minerals vissually. We need to see if the organic is chemically attached to the mineral or if it forms aggregates between them.
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
I'm hoping that someone will be able to help me out with this as I am a biological science person (give me a cell any time).
1- Is there someone in the RTP area that has an SEM with an environmental chamber?
2- Any suggestions on a plastic polymer that could poured onto a bunch of particals (eg: sand) which after polymerization could be fractured and the fractured surface examined by an SEM so that partical size and intrastitial space measurments could be made using image analysis?
3- To complicate matters, it would be nice to seperate (again via SEM) the organics from the minerals vissually. We need to see if the organic is chemically attached to the mineral or if it forms aggregates between them.
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress .. But I repeat myself.-Mark Twain**
One nice carrier I used in the past consisted of a two-part plastic container, where a plastic foil was suspended over the opening of both halves. You would place the sample on the foil in the lower half of the container and close it with the upper half of the container. The sample is then fixed between the two foils which hang suspended in the center of the box.
I don't know who manufacturs this box, but I can find out if you want to. Perhaps somebody else here has a source for this?
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com
} ---------- } From: } "chanch-at-ufl.edu"-at-sparc5.microscopy.com[SMTP:"chanch-at-ufl.edu"-at-sparc5.mi } croscopy.com] } Sent: Wednesday, February 17, 1999 4:50 PM } To: microscopy-at-sparc5.microscopy.com } Subject: TEM sample transport } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } Dear Professional friends, } } I have to use a distant microscope. } What's the best way to transport (from FL to CO) fragile TEM samples? } } best regards, } } chih-hung chang } Dept. of Chemical Engineering } University of Florida } Gainesville,FL 32611 } } }
} Phil, } } I'm having a major problem posting to the Microscopy ListServ would you } post the following for me please. The MSA filter seems to think that I } shouldnt be allowed to post. } } I'm hoping that someone will be able to help me out with this as I am } a biological science person (give me a cell any time). } } 1- Is there someone in the RTP area that has an SEM with an } environmental chamber? } } 2- Any suggestions on a plastic polymer that could poured onto a bunch } of particals (eg: sand) which after polymerization could be fractured } and the fractured surface examined by an SEM so that partical size and } intrastitial space measurments could be made using image analysis? } } 3- To complicate matters, it would be nice to seperate (again via SEM) } the organics from the minerals vissually. We need to see if the organic } is chemically attached to the mineral or if it forms aggregates between } them. } Bob Schoonhoven } rschoonh-at-imap.sph.unc.edu
I am a graduate student at the university of Toronto in the department = of Laboratory medicine . My thesis is a comparative study of the anterior and posterior = cingulate gyrus in Alzheimer's disease and Lewy body dementia . I will = have to count plaques (accumulation of amyloid) and tangles and Lewy = bodies(intracytoplasmic inclusion bodies ) in both the anterior and = posterior portion of the cingulate gyrus which is part of the cortex . = I use immunohistochemisty techniques to stain plaques tangles and Lewy = bodies . I need information (softwares, books,papers, Web sites) to learn about = stereology avoid bias in my count, and take into consideration all the = tissue factors that could affect my count. Thank you for your help. My E.Mail address is mervat.kahil-at-utoronto.ca
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD}
{META content=3Dtext/html;charset=3Diso-8859-1 = http-equiv=3DContent-Type} {META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {FONT size=3D2} I am a graduate student at the university of Toronto = in the=20 department of Laboratory medicine . {/FONT} {/DIV} {DIV} {FONT size=3D2} My thesis is a comparative study of the = anterior and=20 posterior cingulate gyrus in Alzheimer's disease and Lewy body dementia = . I will=20 have to count plaques (accumulation of amyloid) and tangles and Lewy=20 bodies(intracytoplasmic inclusion bodies ) in both the anterior and = posterior=20 portion of the cingulate gyrus which is part of the cortex . I use =
immunohistochemisty techniques to stain plaques tangles and Lewy bodies=20 . {/FONT} {/DIV} {DIV} {FONT size=3D2} I need information (softwares, books,papers, Web = sites) to=20 learn about stereology avoid bias in my count, and take into = consideration all=20 the tissue factors that could affect my count. {/FONT} {/DIV} {DIV} {FONT size=3D2} Thank you for your help. {/FONT} {/DIV} {DIV} {FONT size=3D2} My E.Mail address is {A=20 href=3D"mailto:mervat.kahil-at-utoronto.ca"} mervat.kahil-at-utoronto.ca {/A} {/FO= NT} {/DIV} {/BODY} {/HTML}
I wish to examine the morphology of Desmids (single celled freshwater algae) using SEM. Can anyone give me suitable references for their preparation. Please send them to michaeld-at-amsg.austmus.gov.au
I wish to examine the morphology of Desmids (single celled freshwater algae) using SEM. Can anyone give me suitable references for their preparation. Please send them to michaeld-at-amsg.austmus.gov.au
There have been several recent postings on the use of membrane boxes for the transport and storage of prepared grids. We too are advocates of these boxes, however, with one caveat: You want to be careful that there is nothing on your sample that could react with the thin polyethylene stretched film (membrane).
When even the slightest reaction occurs, the grids are effectively destroyed in terms of their usefulness. Or that is what was described to us by an unhappy customer.
There has been at least one instance where the specimen wanted to stick better to the membrane than to the grid, with the end result that the specimen was lost (stuck) on the membrane and the user was left with a pristine grid sans sample.
We recommend a "test" before storing important samples this way. Some might call it "over kill" but to us it seems like good advice: Take a typical sample and heat age it at 40 deg. C. What ever might happen at room temperature, this test should speed it up about 100 times faster. If a test time about equal to the expected storage or transport time is done, and if on examination, there are no signs of any reaction with the membrane, then you can be reasonably certain that the grid and sample are going to be inert and not changed because of the close proximity to the membrane.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
Dear all, We are imaging pumice sections on glass slides, using BSE detector at 25kv, condenser lens 4, using a Robinson BSE detector on a Hitachi FE4000 The idea is to obtain highly contrasted images to transform into black and white binary images. The problem is we are getting horizontal banding which looks like a charging problem, although the samples are well coated and earthed. Could it be due to anything else? The problem has recently developed and was also apparent on a more conducting sample of some TEM grids. Attached are some images. Any comments would be helpful.
Thank { {horiz_bd.tif} } { {18299_29.tif} } s David Wild
Hi! Does anyone know where I can get grid freezing device for cryo-EM? I am looking for commercially available apparatus that can plunge EM grid into liquid ethane and prepare biological samples in vitrified ice. Thank you very much for your help!
Best regards, Sam Li ******************************************************************************* 213 Wintrobe Building Biochemistry Department University of Utah Salt Lake City, UT 84132 phone: 801-585-5490, fax: 801-581-7959 sam.li-at-m.cc.utah.edu *******************************************************************************
Pardon this belated contribution to the carbon discussion. After returning from the excellent New Zealand Microscopy Conference I found an email were the correspondent wants to exchange graphite for carbon rods. I think that the discussion on those rods was a little incomplete and would like to add this:
Pure (amorphous) carbon rods without graphite (crystalline carbon) would not work; they are essentially non-conductors. If carbon was to volatilise at a much lower temperature than graphite, graphite would be deposited as particulate matter. Clearly that is not so - both carbon and graphite give smooth films under the right conditions. Graphite rods simply have a higher graphite content than carbon rods and purity of analysis is quite another topic.
Higher graphite content rods are more easily shaped or sharpened and since they conduct better than "carbon" rods, they require more current to heat to sublimation point. Of course the diameter of the rods at contact point is another important variable.
I have found that most carbon rod evaporation difficulties are due to wrong voltage selection. Graphite, let alone carbon rods are near impossible to deal with at 10 volts. I used 30 volts and about 60 amps to evaporate from graphite rods. A slightly higher voltage may be preferable for carbon rods. Incidentally 10 volts gives better control for metal evaporation. Clearly maximum amps and an easily selectable range of voltages are a major consideration when purchasing an evaporator.
I am of the persuasion that a strong preference for carbon versus graphite rods is largely a matter of belief. It's too easy to mistake technique and equipment parameters as proving that one or the other rod is "better". I rather state my belief that: Graphite rods are less brittle and carbon cord, because of a larger source area, gives greater uniformity in thickness.
Disclaimer: ProSciTech like all EM suppliers supplies carbon and graphite rods and we have no preference which are purchased. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
Are the images you posted digitally acquired, or from photographs? The bands in your images look just like banding we have had in the past on our Hitachi S-800 FESEM. It drove us nuts for many long months! It appeared in both SE and BSE images, and in all our photographed images. Service personnel could not find the problem. Finally I realized that it really *only* appeared on the recording CRT, and not on the visual CRTs. I hauled out a really old SEM manual from the late 1960s that dealt with all kinds of weirdness. It showed banding on a CRT that was a result of dirty or deficient high voltage to the CRT. I gingerly cleaned the HV contacts to the CRT and the problem disappeared. It reappears every few years, and I imagine it's our humid and somewhat volcanic air that corrodes these contacts. Cleaning them up works. Hitachi remains dubious about this, by the way.
If the bands appear on digital images, I guess you still need to track down where your signal is coming from and see if you have weak contacts.
Good luck! I know how frustrated we were.
Aloha, Tina
On Fri, 19 Feb 1999, D.Wild wrote:
} Dear all, } We are imaging pumice sections on glass slides, using BSE detector at 25kv, } condenser lens 4, using a Robinson BSE detector on a Hitachi FE4000 The } idea is to obtain highly contrasted images to transform into black and } white binary images. The problem is we are getting horizontal banding which } looks like a charging problem, although the samples are well coated and } earthed. Could it be due to anything else? The problem has recently } developed and was also apparent on a more conducting sample of some TEM } grids. Attached are some images. } Any comments would be helpful.
http://www.pbrc.hawaii.edu/bemf/microangela **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
It sure does look like charging (dark) and discharging (bright), but ther= e are other problems which show up in a similar fashion to this when you us= e backscatter.
All this assumes that you do have a good earth on your specimen stage? =
Although BSE see charge far less than SE remember you tend to use much larger currents for BSE observation and you may be trying to cope with th= e large current when you have a very poor earth?
If it is charging one would expect the problem to change in character if you changed the beam current, or more dramatic, change the kV. Lower the=
beam current or the kV and the problem should decrease. Still not convinced then take the sample out and look at a clean stub. No problems then your problem is specimen oriented. If you prove it is charge and t= he stage earth is good -
1. try a faster scan speed for your photography 2. try to work with smaller specimens. 3. try to work at a lower kV (10 or 15) and increase the emission curre= nt (I think a 4000 will go up to 20uA via one of the F keys)
If the above do not markedly change the image form then the problem is probably due to the processing within the backscattered detector system. =
Some BSE detectors allow you to change the response of the amplifier, the= y have viewing and photographic processing options. If you do not have the= se options try taking a photograph at a totally different scan speed?
Good luck
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
======================================================== FOCUS ON MICROSCOPY 1999
12th International Conference on 3D Image Processing in Microscopy 11th International Conference on Confocal Microscopy April 11th-15th, 1999 European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
We wish to announce an important change in the abstract submission and modification deadline. Because of an improved schedule for the printing services of the abstract booklet, we are able to extend the deadline. However, we encourage you to submit and finalise your abstract as quickly as possible since we are starting to arrange the talks into the different sessions.
The new abstract submission and modification deadline is now:
!!! Monday, 8 March 1999 --- 15.00 hours Heidelberg Time !!!
The "abstract submission part" of the database will definitely be closed at 15.00 hours, so that it is not possible to submit further abstracts or make any further modifications to the abstracts after this date.
The "registration only part" of the database will remain open until 9 April 1999!!!
12th International Conference on 3D Image Processing in Microscopy 11th International Conference on Confocal Microscopy April 11th-15th, 1999 European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
Confocal microscopy, multiphoton excitation and deconvolution techniques are increasingly applied in the study of three-dimensional structures such as are encountered in biology, medicine and material sciences. Three-dimensional analysis and representation are crucial tasks in subsequent data assessment. These conferences offer a most efficient meeting point for developers and users working in these rapidly evolving fields and play an important role in the dissemination of information about new developments. Special attention will be given to the dramatic developments in live cell imaging and manipulation, such as the role of the green fluorescent protein. Further information:
Local Organizing Committee: Dr. Ernst H.K. Stelzer, EMBL, Heidelberg Prof. G.J. Brakenhoff, University of Amsterdam Dr. Andres Kriete, University of Giessen
Under the auspices of The International 3D Microscopy Society: Prof. Colin Sheppard, University of Sydney Dr. Andres Kriete, University of Giessen Prof. G.J. Brakenhoff, University of Amsterdam Prof. P-C. Cheng, SUNY at Buffalo Prof. Tony Wilson, University of Oxford Dr. Carol Cogswell, University of Sydney Dr. Vyvyan Howard, University of Liverpool Dr. Guy Cox, University of Sydney Dr. Ernst H.K. Stelzer, EMBL Prof. S. Kawata, Osaka University
We have a Hitachi model S-570 that may be available for sale (80 % sure we will sell, 20 % chance it will be relocated at another Rodel location.). This dual detector tungsten system is in good shape and accepts a 6 in diameter sample. The system will be sold as is. If interested, please e-mail me for more details. Michael Ingram Rodel, Inc Polishing Lab 451 Bellevue Rd Newark, DE 19713 (302) 366-0500, ext.: 2545
I have a question that is not directly related to microscopy but maybe someone out there can help me. One of our customers has asked our company to source and supply a resin used for injection into the vascular system to display the blood vessels (after removing organic material) for teaching, research and museum work.
I have a vague memory of a Japanese resin fulfilling these requirements but can find no immediate reference. Any help gratefully received,
Regards
Terry Cooper TAAB Laboratories Equipment Ltd 3 Minerva House, Calleva Park Aldermaston, Berks, RG7 8NA, UK
Rodel, Inc. had a Hitachi model S-570 that may be available for sale (80 % sure we will sell, 20 % chance it will be relocated at another Rodel location.). This dual detector tungsten system is in good shape and accepts a 6 in diameter sample. The system will be sold as is. If interested, please e-mail me for more details.
Michael Ingram Rodel, Inc Polishing Lab 451 Bellevue Rd Newark, DE 19713 (302) 366-0500, ext.: 2545
"Microwave Techniques for Clinical Histopathology"
There will be a microwave session at Scanning 99 entitled "Microwave Techniques for Clinical Histopathology". Scanning 99 is being held at the Hyatt Regency O'Hare in Rosemont, IL from April 11-14, 1999. The "Microwave Techniques for Clinical Histopathology" session is set for Tuesday, April 13, 1999, in the morning and will consist of invited and contributed presentations. The details of the conference and deadlines/forms for abstract submissions can be found at http://www.scanning.org {http://www.scanning.org} or details can be requested from Mary Sullivan (e-mail: scanning-at-fams.org {mailto:scanning-at-fams.org} , tel: 201-818-1010).
A preliminary list of invited speakers and the titles for the presentation is shown below.
Co-Chairs:
Beverly Giammara - Virtek Vision Inc.
Title "Simple Microwave Methods for Electron Microscopy"
Steven E. Slap - Energy Beam Sciences, Inc.
Title "Introduction to Laboratory Histoprocessing Techniques"
Speakers:
Richard W. Dapson, Ph.D - Anatech Ltd
Title "Microwave Fixation for Histopathology"
D. Denise Hardy - ARUP
Title "Routine Microwave Processing in the Clinical Laboratory"
Linda M. Chicoine - Cognetix/Viatech Imaging, Inc.
Title "Microwave Techniques for Immuno Labeling in Electron Microscopy"
Nathan T. Brinn - Leica Microsystems
Title "Microwave Staining Techniques in Histopathology"
Dear Bob, } } I'm hoping that someone will be able to help me out with this as I am } primarily a biological science person. } } 3- To complicate matters, it would be nice to seperate (again via SEM) } the organics from the minerals vissually. We need to see if the organic } is chemically attached to the mineral or if it forms aggregates between } them. } There are many possibilities for how the organic material could be attached along the continuum from physically adjacent to covalently bonded; however, that said, suspending the specimen in water and applying varying amounts of agitation should distinguish among some of the possi- bilities. If there is only the loosest association between the minerals and the organics, I would expect the former to sink while the latter should float (perhaps you may need to add CsCl to the water if the organ- ics have a specific gravity slightly } 1). If the organics remain attached when the specimen is sonicated, that would signify a tight association. This assumes that neither the minerals nor the organics are water-soluble. Good luck. Yours, Bill Tivol
Our Denton Vaccum Evaporator EV502 won't sputter. We can glow discharge and carbon coat without any trouble but no gold sputtering.
All leads and wiring on the cathode have been changed. We have a new gold target. The cathode was cleaned. =20 The magnets were hopefully put back in the right order. Bioengineering says the electrical system is OK. The vacuum appears OK.
Any suggestions or ideas to get the sputter to sputter=20 would be appreciated.
George Lawton Chief Electron Microscopist Microscopy and Imaging Service Center UT Southwestern Medical Center at Dallas Phone: 214-648-7291 eMail: George.Lawton-at-email.swmed.edu
Terry Cooper wrote: ============================================ I have a question that is not directly related to microscopy but maybe someone out there can help me. One of our customers has asked our company to source and supply a resin used for injection into the vascular system to display the blood vessels (after removing organic material) for teaching, research and museum work.
I have a vague memory of a Japanese resin fulfilling these requirements but can find no immediate reference. Any help gratefully received, ================================================== I am 99% certain that you thinking about the Mercox Resin System which is used pretty universally for corrosion casting studies. It is available from SPI Supplies and some of the other main suppliers of consumables for light and electron microscopy.
You can find out more about the Mercox system from our website URL http: //www.2spi.com/catalog/chem/embed1.html
If you have kept your previous issues of MICROSCOPY TODAY ( Issue 98-7, Sept . 1998), there was an excellent publication by Fred E. Hossler. If you did not save your old issues, there is a link to the electronic version of that publication from the above mentioned URL.
Disclaimer: SPI Supplies distributes the Mercox resin system and therefore we are interested in promoting its use.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
Our Denton Vacuum Evaporator DV502 won't sputter. We can glow discharge and carbon coat without any=20 trouble but no gold sputtering. All the leads and wiring on the cathode have been changed. We have a new gold target. The cathode was cleaned. Magnets were put back in the right order. Bioengineering says the electrical system is OK. The vacuum appears OK. =20
Any suggestions or ideas to get the sputter to sputter would be appreciated.
George Lawton Chief Electron Microscopist Microscopy and Imaging Service Center UT Southwestern Medical Center at Dallas Phone: 214-648-7291 eMail: George.Lawton-at-email.swmed.edu
I can tell you from painful experience to avoid acetone. If you do an acetone rinse on your sample prior to putting it in the box, make sure that all of the acetone has evaporated. If not the sample will stick or you will get a nice hole in the membrane. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
---------- } From: Garber, Charles A. To: MICROSCOPY BB -----------------------------------------------------------------------.
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
There have been several recent postings on the use of membrane boxes for the transport and storage of prepared grids. We too are advocates of these boxes, however, with one caveat: You want to be careful that there is nothing on your sample that could react with the thin polyethylene stretched film (membrane).
When even the slightest reaction occurs, the grids are effectively destroyed in terms of their usefulness. Or that is what was described to us by an unhappy customer.
There has been at least one instance where the specimen wanted to stick better to the membrane than to the grid, with the end result that the specimen was lost (stuck) on the membrane and the user was left with a pristine grid sans sample.
We recommend a "test" before storing important samples this way. Some might call it "over kill" but to us it seems like good advice: Take a typical sample and heat age it at 40 deg. C. What ever might happen at room temperature, this test should speed it up about 100 times faster. If a test time about equal to the expected storage or transport time is done, and if on examination, there are no signs of any reaction with the membrane, then you can be reasonably certain that the grid and sample are going to be inert and not changed because of the close proximity to the membrane.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
The latest edition of the Testing Buyers Guide, compiled by the editors of ASM Internationals Advanced Materials & Processes magazine, is now available online at http://www.asm-intl.org/testing
If you are looking for a SEM supplier, or for a firm to conduct some failure analysis for you, then the place to look is the Equipment/Supplies/Services Listing. Click on the category of interest, and you'll be taken to a listing of companies providing that service. To find the contact details for a particular company, simply click on its name. If you already know the name of a company, then you can proceed directly to the Company Directory. Here you will find an alphabetical listing of the more than 400 companies listed in this directory.
I hope you find this a useful resource.
Leslie H. Chom LChom-at-po.asm-intl.org Manager, Online Services ASM International ph. 440.338.5151 Ext. 5510 fx. 440.338.4634
Need property, composition, and processing data? Alloy Digest Webfaxx --- http://www.asm-intl.org
At 16:23 17/02/99 -0400, you wrote: } Content-Type: text/plain; } charset=3D"utf-8" } X-MIME-Autoconverted: from 8bit to quoted-printable by electra.ciens.ucv.ve id EAA17539 } } Estimados colegas: Para facilitar la difusi=C3=B3n de la informaci=C3=B3n= acerca del } V Congreso Interamericano de Microscop=C3=ADa Electr=C3=B3nica , se la = estamos } enviando bajo tres distintas modalidades (direcci=C3=B3n de la p=C3=A1gina= web, texto } en este correo y un documento anexo en formato Word). Agradecer=C3=ADamos= su } valiosa colaboraci=C3=B3n en ayudarnos a distribuirla. Con un saludo= cordial. } Miren Gonz=C3=A1lez-Elorriaga } } Dear colleagues: In order to facilitate the spreading of the information } relative to the Vth Interamerican Electron Microscopy Congress we are } sending it under three different formats ( web site address, text in this } e-mail, and as an attachment document in Word). We will appreciate very= much } your collaboration in delivering it to other colleagues that could be } interested in attending the Congress. Sincerely yours. Miren } Gonz=C3=A1lez-Elorriaga. } } http://electra.ciens.ucv.ve/~svme/ } } } } SOCIEDAD VENEZOLANA DE MICROSCOPIA ELECTRONICA } UNIVERSIDAD CENTRAL DE VENEZUELA } FACULTAD DE CIENCIAS } CENTRO DE MICROSCOPIA ELECTR=C3=93NICA } } } V INTERAMERICAN } ELECTRON MICROSCOPY } CONGRESS } } VIII VENEZUELAN ELECTRON } MICROSCOPY CONGRESS } } ISLA DE MARGARITA } VENEZUELA } OCTOBER 24 - 28, 1999 } } } } GENERAL INFORMATION } } The forthcoming Interamerican Electron Microscopy Congress is the fifth of= a } series which had its origin in M=C3=A9rida, Venezuela, in 1986. } The scientific program will begin on sunday October the 24th, running } through Thursday October the 28th. The program will feature Simposia, } Invited Talks, Oral Presentations and Posters Sessions related to Electron } Microscopy and its applications to Medicine, Biology and Materials. } We look forward to the participation of colleagues from Latin-America, } North America, Europe and Asia. Given the quality of the participants we } want this event to be very fruitful for both undergraduate and postgraduate } students from the specialities mentioned above. } As usual, we also count with the participation of important Commercial } Companies. } From now we give all participants a warm welcome, wishing them a } nice stay in our beautiful Venezuela. } } } INSTRUCTIONS FOR AUTHORS } } All papers must have a two-page extent. The first page must include only } text; the first 19 lines of the second page must contain the rest of the } text and could also be used to write the References. The remaining area= must } be dedicated to the Figures: graphs, tables and photographs. } } } } We request very specially to follow the format mentioned above. } } Papers must be submitted according to the following guidelines: } Title (Times New Roman, capitals, boldface, 12 points, centered). Leave one } blank line and in the following line write the names of authors,= underlining } the name of the author in charge of the presentation. On the following line } write the institutional affiliation of the authors. Leave two blank lines } and then start writing the text. The text must contain a brief= introduction, } objectives, experimental methods, results, discussion and conclusions.= These } points will be taken into account for the selection of the submitted= works. } Languages: Spanish, English or Portuguese. } Paper size: Letter or A4. } Type font: Times New Roman, 10 points, 1.5 spacing. } Margins: Top (2 cm), Bottom (3.5 cm), Left (3 cm), Right (2 cm). } Please, mail one original and two copies of each paper, together with the } registration form and fee. } Reception of papers will be acknowledged promptly. } } Please, do not send your payment separated from the paper and the } registration form. } } } } } } } } } } REGISTRATION FEES } } SVME / IFSM Members } Until On site } 04/30/99 } } Professionals US$ 200 US$ 230 } Students* US$ 100 US$ 130 } } Non Members of SVME / IFSM } } Until On site } 04/30/99 } } Professionals US$ 250 US$ 280 } Students* US$ 125 US$ 155 } } * Students must include a certificate from their institution stating their } status. } } The registration fee entitles the participant the submission of one paper. } Additional papers will pay a fee of US$ 30. } The registration fee includes a copy of the } proceedings and the participation in all } cultural and social events. } } The registration form, paper and payment must be sent to the following } address: } } V INTERAMERICAN ELECTRON } MICROSCOPY CONGRESS. } Universidad Central de Venezuela } Facultad de Ciencias } Centro de Microscop=C3=ADa Electr=C3=B3nica } Avenida Los Ilustres, Los Chaguaramos, } Caracas, VENEZUELA } } Additional information : } } Tel: 58-2-6052174 } Tel-Fax: 58-2-6930694 } e-mail: } svme-at-electra.ciens.ucv.ve } curbina-at-electra.ciens.ucv.ve } alcastel-at-telcel.net.ve } } PAPERS DATA FORM } This completed form must accompany all papers } } Title: } } } } } } } } } Author (s): } } } } } } Three Keywords: } } } } Poster : } } Space recerved for the poster is } 150 cm.-high by 90cm.-wide. } } } Please return this form with your paper (original and two copies) and the } payment. } } } If the paper is not accepted the author will be refunded a 70% of the } cancelled fee. } } } } } } REGISTRATION FORM } Please fill in Capital letters } } Name: } } Institution: } } Complete Address: } } Phone: FAX: } } e-mail: } } Member of SVME/IFSM: } YES =EF=81=AF NO =EF=81=AF } } Student* =EF=81=AF Professional =EF=81=AF } } } REGISTRATION FEES } } SVME/IFSM Members } Until On Site } 04/30/99 } } Professional US$ 200 US$ 230 } Student* US$ 100 US$ 130 } } SVME/IFSM Non-Members } } Until On Site } 04/30/99 } } Professional US$ 250 US$ 280 } Student* US$ 125 US$ 155 } } * Students must include a copy of their official student I.D. } } } PAPERS DATA FORM } This completed form must accompany all papers } } } } } } } } ORGANIZING COMMITTEE } } President: Dr. Alan Castellano } General Secretary: Dr. Caribay Urbina } Scientific Secretary: Dr. Gema Gonz=C3=A1lez } M.Sc. Sonia Camero. } Dr. Carlos Rojas. } Dr. H=C3=A9ctor Finol. } Dr. Miren Gonz=C3=A1lez } M.Sc. Fredy S=C3=A1nchez } Secretary of Special } Events: Dr. Blanca M=C3=BCller } Treasurer: Dr. Humberto Rojas } Technical Support: Dr. Pedro Rodr=C3=ADguez } } } NATIONAL ADVISORY COMMITTEE } } } } INTEVEP M.Sc.Margarita Navas } IVIC Dr. Ra=C3=BAl Padr=C3=B3n } UCV Dr. Fracehuli Dagger } UCV Dr. Mariana Staia } ULA Dr. Mauro Brice=C3=B1o } LUZ Dr. Orlando Castej=C3=B3n } UNEFM Dr. Auristela de Mirt } IDEA Dr. Gloria Villegas } UDO Dr. Oscar Gonz=C3=A1lez } UDO Dr. Benjam=C3=ADn Hidalgo } USB Dr. Augusto Ruiz } USR Dr. Antonio Breta=C3=B1a } IUT M.Sc. Freddy Arenas } } } } } } } } } } } } COURSES } During October the 24th and the 28th a variety of courses related to } techniques of sample preparation, observation and } microanalysis will be offered. } } Further details about these courses will be in our web site: } http://electra.ciens.ucv.ve/~svme } } COMMERCIAL EXHIBITION } } The Congress will include an exhibition presented by companies that } trade products related to Electron Microscopy. The exhibition area will be } located at the entrance to the halls of conference and close to the posters } area. } } HOTELS } Margarita Hilton International Hotel (Congress Headquarters), use } e- mail or Fax to reserve. 58-95-620810 } http://www.hilton.com } } Details about accomodation will be } informed in our website: } http://electra.ciens.ucv.ve/~svme } } } CALL FOR PAPERS } Deadline: May the 15th 1999 } Notification of acceptance: } from July the 31st 1999 } } } } } } } } Attachment Converted: "C:\FREDDY\Attach\V Interamerican Electron Microscopy Congress.url" } } Attachment Converted: "C:\FREDDY\Attach\VCngIME.doc" }
The latest edition of the Testing Buyers Guide, compiled by the editors of ASM Internationals Advanced Materials & Processes magazine, is now available online at http://www.asm-intl.org/testing
If you are looking for a SEM supplier, or for a firm to conduct some failure analysis for you, then the place to look is the Equipment/Supplies/Services Listing. Click on the category of interest, and you'll be taken to a listing of companies providing that service. To find the contact details for a particular company, simply click on its name. If you already know the name of a company, then you can proceed directly to the Company Directory. Here you will find an alphabetical listing of the more than 400 companies listed in this directory.
I hope you find this a useful resource.
Leslie H. Chom LChom-at-po.asm-intl.org Manager, Online Services ASM International ph. 440.338.5151 Ext. 5510 fx. 440.338.4634
Need property, composition, and processing data? Alloy Digest Webfaxx --- http://www.asm-intl.org
Leslie H. Chom LChom-at-po.asm-intl.org Manager, Online Services ASM International ph. 440.338.5151 Ext. 5510 fx. 440.338.4634
Need property, composition, and processing data? Alloy Digest Webfaxx --- http://www.asm-intl.org
does anybody know the correct setup of the two RS232 remote interfaces on a LEO982. the manual is pretty sketchy on how to actually send and receive data (or i'm just doing something terribly wrong).
i've configured a comm port on a PC to meet the stated specifications of either or both of the RS232 ports on the scope, and i can send data (as seen on a cable monitor) but i don't get any response from the scope. i've used both null modem and standard cable configurations. it seems the ports just are not listening.....
also, does the 982 data screen do anything in response to the remote interface being activated?
any ideas?
thx! brian
____________________________________________________________________ Brian McIntyre mailto:mcintyre-at-optics.rochester.edu Univ. of Rochester-EM Lab 716-275-3058 voice The Institute of Optics 716-244-4936 fax Rochester, NY 14627 http://www.optics.rochester.edu
For some reason I did not see your original message and attachment images that you posted. Therefore I have only seen the responses. I'll throw in my two cents anyway. You mentioned that you have seen the problem using a TEM grid. I would use something like this that is very conductive with strong contrast if you can readily reproduce the problem. If you can reproduce the banding or flashing only with the samples on glass. It is probably your sample. Unless in increasing the contrast(gain in the Robinson BSE), you inadvertently create some "crosstalk" from the voltage for the preamp to a video line.
First of all to eliminate some things a few questions need to be answered (Assuming you have reproduced the banding with a conductive sample). Make sure the grounding plug on the stage near the X Y controls is plugged in correctly. Think back to when the banding started. WAs anybody inside the instrument? Was anything added? Has the BSE detector been adjusted, moved? A wise old service engineer once told me that 80% of all problems are created by someone fiddling with something. After years of service myself he was absolutely right. Do you see the same banding in normal SE mode as well as BSE? If so the Robinson BSE detector is probably not the culprit. Do you see the problem on the view CRT? If so start at a fast raster then step down to slower and slower raster speeds. Take note if the amounts of banding or flashing increases. If so it is probably charging. Photo speeds will show the most charging. If you only see the problem on your photos in SE and BSE then I would suspect the High Voltage to the photo CRT as someone has already suggested. Make sure you know what your doing before messing with this. The anode on the CRT has 8 to 10KV on it. Even after turning the power off it'll hold a considerable shock. If both view and photo CRT's show banding, the problem is visible in SE and BSE, and you have eliminated charging; the high voltage circuit in the high voltage tank has been known to have problems. This is rare however.
} From what little I know I strongly suspect the Robinson has developed a problem. Usually a ground has developoed a poor connection. It has been awhile but I recall a little gold or silver tab that makes contact with the main shaft on the main housing. I don't remember whether you can get to it without taking the housing apart. Make sure this and any other grounds you find have good connections. If you go into the housing make sure the voltage or power cables are seperate from the video cables. I've rambled and can think of nothing else right now. Good luck.
Joel McClintock EM Specialist U of Kentucky (606)257-1242
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Our Denton Vacuum Evaporator DV502 won't sputter. We can glow discharge and carbon coat without any trouble but no gold sputtering. All the leads and wiring on the cathode have been changed. We have a new gold target. The cathode was cleaned. Magnets were put back in the right order. Bioengineering says the electrical system is OK. The vacuum appears OK.
Any suggestions or ideas to get the sputter to sputter would be appreciated.
George Lawton Chief Electron Microscopist Microscopy and Imaging Service Center UT Southwestern Medical Center at Dallas Phone: 214-648-7291 eMail: George.Lawton-at-email.swmed.edu
(InterMail v03.02.07 118 124) with SMTP id {19990219212714.GINO7957-at-oldserver} for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 19 Feb 1999 21:27:14 +0000 Message-ID: {36CDD78A.C0C-at-worldnet.att.net}
Terry Cooper wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } Dear fellow Microscopists, } } I have a question that is not directly related to microscopy but maybe } someone out there can help me. One of our customers has asked our company } to source and supply a resin used for injection into the vascular system to } display the blood vessels (after removing organic material) for teaching, } research and museum work. } } I have a vague memory of a Japanese resin fulfilling these requirements but } can find no immediate reference. Any help gratefully received, } } Regards } } Terry Cooper } TAAB Laboratories Equipment Ltd } 3 Minerva House, Calleva Park } Aldermaston, Berks, RG7 8NA, UK
Dear Terry Cooper,
The product I believe you are referring to is Mercox. It is available from Ladd in Red (#21245), Blue (#21246), and Clear (#21247).
John Arnott Chairman
-- LADD RESEARCH 13 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
I would like to reiterate what Joel McClintock mentioned in his post, and which I neglected to point out. The high voltage supply to your CRT is 10 kV! Be careful! The cleaning I have done has so far not involved actually polishing contacts, but merely blowing compressed clean, dry air over the contacts, making sure they are seated, cleaning the surrounding areas, and carefully closing everything up again.
There are a number of circuits in most instruments that are still energized or retain a charge when the instrument is OFF or even DISCONNECTED.
I hate having a perfectly good, sunny, warm, Hawaiian winter day with great surf ruined by getting thrown up against the wall and electrocuted.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Dear Bob, I cannot help you with the location of the variable-pressure SEM, but the sample prep I can. 2. The usual practice is to mount the sample in a quick-setting epoxy resin, with most of the sample in the bottom. I use silicon rubber cups one inch in diameter, about 1/2 inch deep. The set resin is then ground on a graded series of sandpaper disks, then polished on diamond suspension to cross-section the particles. The sample is then gold or carbon coated. 3. organic and minerals are easily separated by backscattered imaging (BSE). The organics, being composed of light elements, will show darker than the mineral, which is composed of heavier elements. A photo of SEM and BSE of the same area is often helpful. Particle size measurements may be better done on a sample of grains sprinkled on a sticky tab and iamged by BSE. Hope this helps. You wrote: } I'm hoping that someone will be able to help me out with this as I am } primarily a biological science person. } } 1- Is there someone in the RTP area that has an SEM with an } environmental chamber? } } 2- Any suggestions on a plastic polymer that could poured onto a bunch } of particals (eg: sand) which after polymerization could be fractured } and the fractured surface examined by an SEM so that partical size and } intrastitial space measurments could be made using image analysis? } } 3- To complicate matters, it would be nice to seperate (again via SEM) } the organics from the minerals vissually. We need to see if the organic } is chemically attached to the mineral or if it forms aggregates between } them. } } best regards, } Bob } Robert Schoonhoven } Laboratory of Molecular Carcinogenesis and Mutagenesis } Dept. of Environmental Sciences and Engineering } University of North Carolina } CB#7400 } Chapel Hill, NC 27599 } Phone } office 919-966-6343 } Lab 919-966-6140 } Fax 919-966-6123 } } **Suppose you were an idiot... And suppose you were a member of Congress } .. } But I repeat myself.-Mark Twain** } Regards, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
} Date: Fri, 19 Feb 1999 15:48:43 -0800 } To: "rschoonh-at-sph.unc.edu"-at-Sparc5.Microscopy.Com, Microscopy } From: Mary Mager {mager-at-interchange.ubc.ca} } Subject: Re: material sciences question } } Dear Bob, } I cannot help you with the location of the variable-pressure SEM, but the sample prep I can. } 2. The usual practice is to mount the sample in a quick-setting epoxy resin, with most of the sample in the bottom. I use silicon rubber cups one inch in diameter, about 1/2 inch deep. The set resin is then ground on a graded series of sandpaper disks, then polished on diamond suspension to cross-section the particles. The sample is then gold or carbon coated. } 3. organic and minerals are easily separated by backscattered imaging (BSE). The organics, being composed of light elements, will show darker than the mineral, which is composed of heavier elements. A photo of SEM and BSE of the same area is often helpful. } Particle size measurements may be better done on a sample of grains sprinkled on a sticky tab and iamged by BSE. } Hope this helps. } You wrote: } } I'm hoping that someone will be able to help me out with this as I am } } primarily a biological science person. } } } } 1- Is there someone in the RTP area that has an SEM with an } } environmental chamber? } } } } 2- Any suggestions on a plastic polymer that could poured onto a bunch } } of particals (eg: sand) which after polymerization could be fractured } } and the fractured surface examined by an SEM so that partical size and } } intrastitial space measurments could be made using image analysis? } } } } 3- To complicate matters, it would be nice to seperate (again via SEM) } } the organics from the minerals vissually. We need to see if the organic } } is chemically attached to the mineral or if it forms aggregates between } } them. } } } } best regards, } } Bob } } Robert Schoonhoven } } Laboratory of Molecular Carcinogenesis and Mutagenesis } } Dept. of Environmental Sciences and Engineering } } University of North Carolina } } CB#7400 } } Chapel Hill, NC 27599 } } Phone } } office 919-966-6343 } } Lab 919-966-6140 } } Fax 919-966-6123 } } } } **Suppose you were an idiot... And suppose you were a member of Congress } } .. } } But I repeat myself.-Mark Twain** } } } Regards, } Mary } Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
With a little more time I have re read your mail and the difficulty of coating your specimen suddenly hits me in the eye!
At the kV you are using (25) with the complicated surface of your specime= n you would have to go to some pretty complex sputtering techniques to remo= ve the possibilities of charging.
If it was me I would conduct the empty stub test I mentioned in an earlie= r =
mail and if this was OK I would then look at the size and shape of the specimen and the coating method.
Sputter coaters are not as good as people like to think they get beaten b= y complex surfaces when a conducting path to earth is very very difficult t= o achieve.
Try this
1. Coat the specimen for a minute or so at 45 degrees 2. Repeat with it tilted 45 degrees in exactly the opposite directi= on 3. Get the best vacuum that you can, by turning off the sputter gas,=
and try to sputter again.
See if this works as it is my standard method for very difficult specimen= s. I find this is the only way to bury the metal deep into the specimen por= es but you should also lower the kV!
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
I would also add my two cents to Mary Mager response to adding epoxy to sand/organic mixture. To eliminate voids, we would evacuate the powder sample and then add the liquid via a tube from the outside. The liquid had been previously degassed by either heating or evacuation. The apparatus is simple to construct. Our high resolution density measurements indicated almost complete filling of all voids limited only by the molecular size of the liquid.
Another technique is to simply put the powder and epoxy together in a vaccuum oven and then very slowly evacuate. Make sure there is plenty of room for the bubbles in the sample holder.
J. Roy Nelson, PhD Material Testing Laboratory Pennington, NJ (609) 730-0575 jrnelson-at-nj1.aae.com
-----Original Message----- } From: Tina Carvalho [SMTP:tina-at-pbrc.hawaii.edu] Sent: Saturday, February 20, 1999 12:22 AM To: Joel McClintock Cc: microscopy-at-Sparc5.Microscopy.Com
Hi, all-
A researcher has approached me with confocal images of part of an insect's nervous system that has cells that particularly light up with FITC. They don't know what these cells are, and they want to be able to locate these cells in the TEM. They are looking for something in particular, but I am not allowed to be more specific.
Their idea is to have HRP-conjugated anti-FITC, hoping for a dark reaction product that I will be able to find in the TEM.
I haven't done any HRP staining in (yikes!) decades, and so do not have a protocol handy. Could someone suggest one or, perhaps better, an alternative plan? I am very open to suggestions! Since all they know about these cells is that they fluoresce with FITC, I haven't come up with any other labelling ideas. When I asked if they could microinject the cells, I got blank and then fearful stares.
Thanks in advance!
Mahalo, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Before taking my new position as an educator who uses the SEM to improve high school curriculum, the SEM affected my life in incidental ways. For example, the SEM allowed scientists to study and improve their understanding of biology, medicine. Material manufacturing and the aerospace industry used scanning electron microscopes to improve manufacturing processes and to maintain the global superiority of the United States Air Force. I was aware of its use and power, but did not have much contact with the technology.
I was privileged, as a teacher, to take classes on field trips to local industry to use their SEM. Once, a class engaged in a project to determine the damage done by painting the ceiling tiles in our high school's cafeteria. We did sound spectrum studies and painted acoustical tile with unaltered tile under the scanning electron microscope. As you can imagine, the paint had filled in most of the sound-absorbing gaps, reducing the effectiveness of the tiles.
More recently, I have been using a "Personal Scanning Electron Microscope" to improve the curriculum in Dayton area schools. We have used the SEM to examine the adaptations of stream invertebrates to various functions in their ecosystem, to compare the structure of red blood cells (eukaryotes) to blue- green algae (prokaryotes), to examine the pollen comb, brush, and pocket on the hind legs of honeybees, and to measure microscopically.
We have also described the crystalline forms of rocks and minerals and studied microfossils found in diatomaceous earth. I am currently at the Museum of Discovery in Dayton, Ohio, where we are photographing tiny ants and snails that have never been seen under an SEM before. Some of these images will be published in scientific journals to advance our understanding of the diversity of our environment while others will be archived along with the actual specimens for the benefit of future generations.
Even if I had never had the opportunity to use an SEM, it would have been improving my life indirectly through the contributions of scientists who do use one. Having the opportunity to use one several days a month is enriching my life as I am able to work and contribute on the border between science and art, using both sides of my brain!
Good luck with your report, Amy!
Sincerely,
Carlton Bowers Alliance for Education TECH TREK Mobile Research Laboratory Curriculum & Technology Specialist (937) 222-2934
George Lawton wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Our Denton Vaccum Evaporator EV502 won't sputter. } We can glow discharge and carbon coat without any } trouble but no gold sputtering. } } All leads and wiring on the cathode have been changed. } We have a new gold target. The cathode was cleaned. } The magnets were hopefully put back in the right order. } Bioengineering says the electrical system is OK. The } vacuum appears OK. } } Any suggestions or ideas to get the sputter to sputter } would be appreciated. } } George Lawton } Chief Electron Microscopist } Microscopy and Imaging Service Center } UT Southwestern Medical Center at Dallas } Phone: 214-648-7291 } eMail: George.Lawton-at-email.swmed.edu
George, I had this happen on my Polaron 5100. Try going to full voltage at a very poor vacuum and see if you can make it start. It may have something to do with contamination.
Ken Converse owner Quality Images third party SEM service Delta, PA
Bob, At the risk of running on- I'd just like to add my two cents worth to the good advise that you have received from Mary Mager.
Particle Size We have had the best results determining particles size by the "keep it simple" rule for SEM preparations. We usually use the sticky tab method. One of the commercially available, double sided carbon adhesives such as SPI Carbon Tape or Ted Pella Carbon Conductive Tabs work well. SEM secondary electron imaging (SEI) usually does great, but you will probably have great backscattered electron contrast as well (BEI). This simple prep works best (for us) for the particle size aspect of the problem, because you needn't worry yourself with the problem of recognizing small particle fragments due to the fracturing or grinding/polishing.
Composition/ Organic and inorganic As Mary recommends, you will want to embed and cross-section (by grinding /polishing) to expose a topography free surface. SEM-BEI will show the spatial correlation between the mineral and carbonaceous components. Also don't overlook PLM. If you have access to a good optical microscope, you may learn much about the structure here.
Intrastitial and interstitial spaces Again the cross-section prep is your best shot. I use Buehler's EPO-COLOR fast cure epoxy for these application. It is a nice bright red color that will likely be different in color fron any other components in your sample. Use a vacuum infiltration before curing. This allows examination by optical techniques with differentiation between the epoxy embedding material and the other organics in the specimen - works great for identifying porosity. Then on to the SEM for a closer look and identification of compositions.
Hopefully SEM has the resolution to examine the intrastitial space that your interested in. Otherwise its on to the TEM. isn't it fun?! Regards, Brad Huggins
Mary Mager Wrote: Dear Bob, I cannot help you with the location of the variable-pressure SEM, but the sample prep I can. 2. The usual practice is to mount the sample in a quick-setting epoxy resin, with most of the sample in the bottom. I use silicon rubber cups one inch in diameter, about 1/2 inch deep. The set resin is then ground on a graded series of sandpaper disks, then polished on diamond suspension to cross-section the particles. The sample is then gold or carbon coated. 3. organic and minerals are easily separated by backscattered imaging (BSE). The organics, being composed of light elements, will show darker than the mineral, which is composed of heavier elements. A photo of SEM and BSE of the same area is often helpful. Particle size measurements may be better done on a sample of grains sprinkled on a sticky tab and iamged by BSE.
You wrote: } I'm hoping that someone will be able to help me out with this as I am } primarily a biological science person. } } 1- Is there someone in the RTP area that has an SEM with an } environmental chamber? } } 2- Any suggestions on a plastic polymer that could poured onto a bunch } of particals (eg: sand) which after polymerization could be fractured } and the fractured surface examined by an SEM so that partical size and } intrastitial space measurments could be made using image analysis? } } 3- To complicate matters, it would be nice to seperate (again via SEM) } the organics from the minerals vissually. We need to see if the organic } is chemically attached to the mineral or if it forms aggregates between } them. } } best regards, } Bob } Robert Schoonhoven
Hi all, I have recently finished my third year project in electron microscopic observations of the entry and exit of influenza virus. In it I used Thermanox coverslips to culture the cells on, one of the problems that I had was delamination of the coverslip from the resin (Spurrs) and wondered if this had any thing to do with the different densities of the Thermanox and resin. If anyone knows their densities I would be most grateful.
I'm back on-line, albeit remotely. I have noticed several recent postings this week (of course) in which users have appended images to their mailings.
Please remember this is not accepted practice on this listserver. Too many people have low bandwidth or expensive or slow connections. Fortunately for me, my software is set to ignore large downloads while I'm on the road and operating via a modem. Many users do not have this luxury. This is, of course, detailed in the rules of listserver usage which you all have received when you subscribed.
If you wish to share images with list users place them on a WWW site and just provide a URL in the body of your message..
I am putting the final touches on a presentation on the state of microscopy education for a presentation this coming Thursday (New York Microscopical Society) and would love to include some good "up-to-date" stats about what's going on in the field. While I have a lot of material on courses at Delta San Joaquin, Woods Hole, Lehigh, and through MSA, I could use some info "from the field". If you are teaching a course at your company/university, could you please send (email or snail mail) a quick synopsis? Also of interest: if you are including a microscopy unit as part of another course.
Many thanks! Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
Michael: I worked with Desmids more than a quarter of a century ago. Cannot lay may hands on those reference now but the important things learned about their preparation for SEM would not have changed!
These cells are very sensitive to osmotic shock. We used the buffer quite dilute - 0.01M at the most and dehydration has to be slower than normal, have more gradual steps and begin with about 20% ethanol, when most other specimens can tolerate dehydration from 70%. Since these are large cells you can observe osmotic effects under a light microscope.
I think that we used our improvised freeze drying method back then, but solvent drying or critical point drying too, if you can retain the cells should give good results. Call me if you have further questions. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Friday, February 19, 1999 1:24 PM, MichaelD [SMTP:michaeld-at-amsg.austmus.gov.au] wrote: } ---------------------------------------------------------- } -------------- } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht } ml } ---------------------------------------------------------- } -------------. } } } I wish to examine the morphology of Desmids (single } celled freshwater } algae) using SEM. Can anyone give me suitable } references for their } preparation. } Please send them to michaeld-at-amsg.austmus.gov.au } } Thanking you in anticipation } } } Mike Dingley.
Does anyone have a reference or recipe for post-fixing with acetate-veronal (AV)-buffered OsO4, and/or en bloc staining with AV-buffered UA?? I believe Palade authored the technique, probably others have modified it.
Thanks, Ann Lehman Trinity College Hartford, CT v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-exchange.cc.trincoll.edu
We have a new semester course in EM. It covers theory and application of both SEEM and TEM. It is an undergraduate course, at the 5000 level (senior, graduate school acceptable). Students prepare and view their own samples on the Hitachi S4000 SEEM and on the Zeus 902 TEM. Digital images are generated as TIFF files and the students produce portfolios with PhotoShop. Final prints are made with an Alps color printer. We are establishing a web site, and student work will be posted on this site.
William McManus, MS Lab Supervisor Electron Microscope Facility Department of Biology Utah State University Logan UT 854322-5305
435-797-1920
-----Original Message----- } From: Barbara Foster [mailto:mme-at-map.com] Sent: Sunday, February 21, 1999 5:42 PM To: Confocal Microscopy List; microscopy-at-Sparc5.Microscopy.Com
Dear list-listeners,
I am putting the final touches on a presentation on the state of microscopy education for a presentation this coming Thursday (New York Microscopical Society) and would love to include some good "up-to-date" stats about what's going on in the field. While I have a lot of material on courses at Delta San Joaquin, Woods Hole, Lehigh, and through MSA, I could use some info "from the field". If you are teaching a course at your company/university, could you please send (email or snail mail) a quick synopsis? Also of interest: if you are including a microscopy unit as part of another course.
Many thanks! Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
Tina Carvalho wrote: } } A researcher has approached me with confocal images of part of an insect's } nervous system that has cells that particularly light up with FITC. } They don't know what these cells are, and they want to be able to locate } these cells in the TEM. They are looking for something in particular, but } I am not allowed to be more specific. } } Their idea is to have HRP-conjugated anti-FITC, hoping for a dark reaction } product that I will be able to find in the TEM. } } I haven't done any HRP staining in (yikes!) decades, and so do not have a } protocol handy. Could someone suggest one or, perhaps better, an } alternative plan? I am very open to suggestions! Since all they know } about these cells is that they fluoresce with FITC, I haven't come up with } any other labelling ideas. When I asked if they could microinject the } cells, I got blank and then fearful stares. } Dear Tina, Jim Turner has done a lot of corellative confocal LM and EM. Try some of his publications for a protocol. 10 kV would ruin one of your wonderful Hawaiian days; we just use the shock to recover from Albany winter :-). Yours, Bill Tivol
This is a multi-part message in MIME format. --------------3B1287CCAA802B61D5BACA63 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit
Dear Dr. Carvalho
An alternative tehcnique to anti-FITC antibodies in electron microcopy may be the photoconversion of DAB by the irradiation of the FITC-marked cells using a laser or a fluorescent microscope. I never used this technique but it appears to be relatively easy. This technique is described by Pagano and Martin (1997) in Cell Biology: A Laboratory Handbook, 2nd ed., Julio E Celis, Ed: 505-510, Academic Press, San Diego.
I hope it works Good Luck!
Francisco Javier Hernandez-Blazquez IARC-WHO Unit of Multistage Carcinogenesis 150 Cours Albert Thomas Lyon - France
fhernandez-at-iarc.fr
Tina Carvalho wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi, all- } } A researcher has approached me with confocal images of part of an insect's } nervous system that has cells that particularly light up with FITC. } They don't know what these cells are, and they want to be able to locate } these cells in the TEM. They are looking for something in particular, but } I am not allowed to be more specific. } } Their idea is to have HRP-conjugated anti-FITC, hoping for a dark reaction } product that I will be able to find in the TEM. } } I haven't done any HRP staining in (yikes!) decades, and so do not have a } protocol handy. Could someone suggest one or, perhaps better, an } alternative plan? I am very open to suggestions! Since all they know } about these cells is that they fluoresce with FITC, I haven't come up with } any other labelling ideas. When I asked if they could microinject the } cells, I got blank and then fearful stares. } } Thanks in advance! } } Mahalo, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } ****************************************************************************
--------------3B1287CCAA802B61D5BACA63 Content-Type: text/x-vcard; charset=us-ascii; name="fhernandez.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Francisco Hernandez Content-Disposition: attachment; filename="fhernandez.vcf"
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Hi Barbara, In brief: We teach TEM, SEM, and Electron Diffraction courses open to gradurates and undergraduates. The TEM course is a 6 hr. credit course (2hrs/lecture, 4hrs/lab) that involves producing a report which is 50% of their grade. The main challenge is producing good sections with a tempermental 21 year old ultramicrotome. The SEM course is for 2 hrs. credit again is with 50% of the grade on their report. Here we are at mercy of our 25 year old ETEC behaving well. The EM Diffraction course is taught only every few years. But every year we offer a one afternoon EM Diffraction Lab as part of a Solid State Chemistry Techniques course. In addition we conduct highschool tours, and have involved high school students in special summerschool projects here on the University campus. On occasion we have trained researchers from industry in EM instrumentation techniques. The microscopy courses serve important functions in that some of my former students have gone on to direct EM or microscopy imaging facilities in industry and Universities, and others in their capacity as teachers or researchers will be able to share and expand the microscopy knowledge with others. Our concerns: 1.) Because of the small class size (6-10 students on average) there is pressure to drop the courses. Yet it is usually the students who end up doing the research for faculty. 2.) Departments faced with budget concerns want to cut service contracts. This is especially determental to TEM performance and the production of quality results. 3.) Lack of interest in replacing, repairing and/or upgrading equipment, and support for training courses for staff in new microscopy techniques. Suggestion: For MSA or local Microscopy Societies to produce a brochure, or better, a series of brochures on different microscopy and imaging techniques and their relevance and applications to research and commerce aimed at high school level science students. If these were available to hand out for high school tours or accessable to high school teachers, it would do much in furthering the interest and understanding of microscopy.
Barbara Foster wrote: } Dear list-listeners, } I am putting the final touches on a presentation on the state of microscopy } education for a presentation this coming Thursday (New York Microscopical } Society) and would love to include some good "up-to-date" stats about } what's going on in the field. While I have a lot of material on courses at } Delta San Joaquin, Woods Hole, Lehigh, and through MSA, I could use some } info "from the field". If you are teaching a course at your } company/university, could you please send (email or snail mail) a quick } synopsis? Also of interest: if you are including a microscopy unit as part } of another course. } } Many thanks! } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis.
Henry Eichelberger, EM Facility Manager Department of Biological Sciences Binghamton, University
I have been giving a course since 1993 to biomedical graduate students here at the University of Michigan entitled "Morphology for Molecular Biologists." You can get an idea of the course and see a schedule in this URL:
http://www.umich.edu/~mmb533/
Unfortunately, you won't be able to view the lectures, which have to be restricted to the University of Michigan, because they contain some copyrighted material.
Kent
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen Department of Anatomy and Cell Biology, Medical Sciences II Building University of Michigan Medical School, Ann Arbor, MI 48109-0616 akc-at-umich.edu, Tel (734) 763-1287, Fax (734) 763-1166 http://www.umich.edu/~akc/
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear list-listeners, } } I am putting the final touches on a presentation on the state of microscopy } education for a presentation this coming Thursday (New York Microscopical } Society) and would love to include some good "up-to-date" stats about } what's going on in the field. While I have a lot of material on courses at } Delta San Joaquin, Woods Hole, Lehigh, and through MSA, I could use some } info "from the field". If you are teaching a course at your } company/university, could you please send (email or snail mail) a quick } synopsis? Also of interest: if you are including a microscopy unit as part } of another course. } } Many thanks! } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis. } }
Since I am the person who, I think, started this discussion, I'd like to explain why I am developing a preference (purely personal) for "carbon" over "graphite". It may help others.
My coater, an old Edwards 306, uses a 3mm sharpened rod which is caused, by a spring, to bear on to a flat-ended 0.25" carbon rod (which I call the anvil). The 3mm (consumable) rod is held in a hole, about 8mm long, drilled into the end of another piece of 0.25" rod (pity about the ban on images).
When I was using my previous supply of "National" spectroscopic carbon rods, the anvil and the holder used to last for about a year. When I switched (somewhat unwittingly) to "graphite" rods, the higher temperature (or maybe it was the higher current) caused both the holder and the anvil to get so hot that the former lasted for three coatings and the latter for five.
I could, of course, modify my holder and anvil set-up, but it seems to me that it would be easier just to try to obtain some rods similar to those which I had before.
I'm sure there must be other coaters can cope with the graphite rods better than mine can.
It's not just "belief", Jim, but rather, as you also say, different operating and instrument parameters.
Disclaimer: As a hard-pressed University employee, I just want things which work satisfactorily to continue to do so with the minimum hassle factor.
cheers
Ritchie
} From: Jim J Darley {jim-at-proscitech.com.au} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au} } To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com} } Subject: Carbon versus graphite rods } Date: Fri, 19 Feb 1999 14:52:38 +1100 } Organization: proscitech
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Pardon this belated contribution to the carbon discussion. } After returning from the excellent New Zealand Microscopy } Conference I found an email were the correspondent wants to } exchange graphite for carbon rods. I think that the } discussion on those rods was a little incomplete and would } like to add this: } } Pure (amorphous) carbon rods without graphite (crystalline } carbon) would not work; they are essentially } non-conductors. If carbon was to volatilise at a much lower } temperature than graphite, graphite would be deposited as } particulate matter. Clearly that is not so - both carbon } and graphite give smooth films under the right conditions. } Graphite rods simply have a higher graphite content than } carbon rods and purity of analysis is quite another topic. } } Higher graphite content rods are more easily shaped or } sharpened and since they conduct better than "carbon" rods, } they require more current to heat to sublimation point. Of } course the diameter of the rods at contact point is another } important variable. } } I have found that most carbon rod evaporation difficulties } are due to wrong voltage selection. Graphite, let alone } carbon rods are near impossible to deal with at 10 volts. I } used 30 volts and about 60 amps to evaporate from graphite } rods. A slightly higher voltage may be preferable for } carbon rods. Incidentally 10 volts gives better control for } metal evaporation. Clearly maximum amps and an easily } selectable range of voltages are a major consideration when } purchasing an evaporator. } } I am of the persuasion that a strong preference for carbon } versus graphite rods is largely a matter of belief. It's } too easy to mistake technique and equipment parameters as } proving that one or the other rod is "better". I rather } state my belief that: Graphite rods are less brittle and } carbon cord, because of a larger source area, gives greater } uniformity in thickness. } } Disclaimer: ProSciTech like all EM suppliers supplies } carbon and graphite rods and we have no preference which } are purchased. } Cheers } Jim Darley } ProSciTech Microscopy } PLUS } PO Box 111, Thuringowa QLD 4817 Australia } Phone +61 7 4774 0370 Fax: +61 7 4789 2313 } Great microscopy catalogue, 500 Links, MSDS, User Notes } ********************** www.proscitech.com.au ***** }
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
With regard to your quest for information on training organisations.
Protrain, based in Oxford England, and its sister company SSEM, based in California, have been running "in house" electron microscopy training courses around the world for the past eighteen years.
Our staff are made up of professional electron microscopists who have bee= n in the business for at least 33 years. We all started out as service engineers in the days when you literally built the instruments on the customers site. Our paths then moved toward those of application specialists, combining instrument demonstration and customer training, these roles with a number of the most well known manufacturers. Since we=
moved into running our own training organisations we have run courses in university and industrial organisations covering all the natural English speaking countries in the world and some where English was not their firs= t language.
Our training courses are run on customer demand in the clients laboratory= , but we are often employed by universities to run specific courses for the= m within their own E.M. Units. Many of our courses will be similar to othe= rs that you may hear about in your survey, but I believe that we are the onl= y organisation in the world than run courses for technical staff on the maintenance of electron microscopes. Our latest move has been to make th= e courses available with sound on CD ROM, taking information to those that are unable to afford the hands on training.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
If it is not a violation of state laws or institutional policy to provide the information, I am interested in determining the extent to which laboratories in U. S. academic institutions regulate their chemical waste and provide radiation safety protection and laser safety protection policies and procedures for their employees. Are appropriate OSHA and other regulatory agency rules mandated by your institutions? Have some been granted extensions in order to set up appropriate procedures?
I would specifically like to know if anyone has direct knowledge of laboratory safety inspections by the EPA (Environmental Protection Agency) or OSHA (Occupational Safety and Health Administration) or any other state or federal safety regulating agency. If so, what has been the outcome of the inspections? Have fines have been levied against institutions for improper chemical storage or waste disposal procedure violations? Have any fines been imposed for radiation or laser safety violations? Obviously, this listserver is primarily microscopy lab directed, however, it would be useful to know if laboratories other than electron microscope labs have been inspected as well.
The purpose of my request is to gather data for a talk I will be giving. I would like to know how rigorously regulatory agency rules are inforced for academic institutions compared to industry enforcement.
Our institution has a strict waste disposal policy. No chemical is flushed down the sink. All waste is put in appropriate containers and is removed by university Risk Management people as needed. State inspectors have inspected laser labs. This resulted in a request for better beam stop protection. I am not aware of any fines. To date, there have been no OSHA or EPA inspections. Our Radiation Protection Office oversees radiation and laser safety on campus and provides excellent protection resources.
All answers will be reported generically. If any information is collected and if a summary is requested, no institution will be listed in my talk or in a summary to this listserver. Respondents may wish to reply to me directly.
John C. Wheatley Lab Manager Arizona State University Center for Solid State Science PSA-213 BOX 871704 Tempe, AZ 85287-1704
We offer a two year Associate's Degree in Electron Microscopy at Madison Area Technical College in Madison, Wisconsin. The four semester program deals with SEM, FESEM, TEM, AFM, sample prep for materials and biologic, and basic EM maintenance. Check out our web site for more info: http:// Electron-Microscopy.madison.tec.wi.us
Bill Carmichael EM Program MATC 3550 Anderson St. Madison, WI 53704 billc-at-jvlnet.com
Barbara Foster wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear list-listeners, } } I am putting the final touches on a presentation on the state of microscopy } education for a presentation this coming Thursday (New York Microscopical } Society) and would love to include some good "up-to-date" stats about } what's going on in the field. While I have a lot of material on courses at } Delta San Joaquin, Woods Hole, Lehigh, and through MSA, I could use some } info "from the field". If you are teaching a course at your } company/university, could you please send (email or snail mail) a quick } synopsis? Also of interest: if you are including a microscopy unit as part } of another course. } } Many thanks! } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis.
We offer a two year Associate's Degree in Electron Microscopy at Madison Area Technical College in Madison, Wisconsin. The four semester program deals with SEM, FESEM, TEM, AFM, sample prep for materials and biologic, and basic EM maintenance. Check out our web site for more info:
http://Electron-Microscopy.madison.tec.wi.us
Bill Carmichael EM Program MATC 3550 Anderson St. Madison, WI 53704 billc-at-jvlnet.com
Barbara Foster wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear list-listeners, } } I am putting the final touches on a presentation on the state of microscopy } education for a presentation this coming Thursday (New York Microscopical } Society) and would love to include some good "up-to-date" stats about } what's going on in the field. While I have a lot of material on courses at } Delta San Joaquin, Woods Hole, Lehigh, and through MSA, I could use some } info "from the field". If you are teaching a course at your } company/university, could you please send (email or snail mail) a quick } synopsis? Also of interest: if you are including a microscopy unit as part } of another course. } } Many thanks! } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis.
A very big hello to everyone that I haven't spoken to since leaving the Microscopy List some 2 years ago. My new vocation leads me to re-subscribing to ask you all a very big favour.
***Have you done any EM work (imaging or analytical) for archaeologists? If so, would you be so kind as to drop me a line to let me know the aim and nature of the work? Reasons follow;
I left the Microscopy Labs of the Australian Museum to follow my wife's work in Brussels, Belgium, where I'm doing a Ph.D. in Archaeology (in 3D modelling). The undergraduate courses in our faculty do not cover many scientific techniques, so my fellow students have badgered me into giving a seminar on EM's and their applications in archaeology, and a demo on the uni's SEM. I have plenty of examples from my work in zoology, but obviously I need examples from archaeology. I have some references to work with but I figure that the BEST references are the people on this List.
Please understand that my motivations are honest. My only use of your information will be this once-off seminar for my fellow students. They are undergrads who are not in a position to poach any research for their own advantage. All contributors will be acknowledged. And I will be personally very grateful for your help.
We recently purchased Pixera Professional digital camera (for optical microscopy). Nice little thing, but:
We want to have the camera on PC (Pentium II, 333MHz, 128MB RAM) with Win NT 4.0 operating system. We successfully installed the NT driver Pixdrv.drv and Pixera Visual Communication Suite (from a CD). After starting Studio Viewfinder (a software for picture acquisition), there was just fine b/w raster (noise) instead of picture. Using other software and Twain interface led us to the same disappointment. We repeated experiments with both service packs for NT 4.0, namely 3 and 4.
We also tried to install the same camera on another computer (Pentium MMX 200MHz, 64MB RAM ), running on Win 98. New hardware was installed without a problem, but when opening the Pixera VC Suite and running Viewfinder program a message "Can not initialize the camera" appeared.
I would be very grateful for any suggestion.
Best regards,
Goran Drazic
------------------------------
Dr. Goran Drazic J. Stefan Institute Jamova 39 SI-1001 Ljubljana Slovenia
{!doctype html public "-//w3c//dtd html 4.0 transitional//en"} {html} I have a surplus 3.5 mm {b} never been used {/b} Diatome diamond knife old style boat for sale. {/html}
In a message dated 99-02-23 08:57:29 EST, Goran.Drazic-at-ijs.si writes:
{ { We also tried to install the same camera on another computer (Pentium MMX 200MHz, 64MB RAM ), running on Win 98. New hardware was installed without a problem, but when opening the Pixera VC Suite and running Viewfinder program a message "Can not initialize the camera" appeared.
} }
Dr. Drazic,
I had the same problem just yesterday on a new Pixera installation. Pixera told me this is a problem with some high-speed computers.
We switched out the interface card to a "Cirrus Logic PCIC compatible PCI to PCMCIA Bridge," and I had to load a new set of drivers from a floppy disk that was included with the PCI card from Pixera. I also found out that I did not have "32 bit support" enabled on the computer. With the new card and 32 bit support enabled, the camera now works beautifully.
If your local representing agency can not help, I suggest you contact Pixera directly at:
www.pixera.com
} From there you can contact Technical Support. The new set of drivers are two fairly small files. If you can get the correct PCI board, I am sure Pixera could send them to you via e-mail.
Good luck, I hope this helps!
Best regards,
Bob ****************************** Robert (Bob) Chiovetti, Ph.D. President Microimaging Technologies, Inc. Tucson, Arizona USA Tel./Fax (520) 546-4986 rchiovetti-at-aol.com Manufacturers' Representatives Systems Integrators Analog & Digital Imaging Systems Clinical & Research Microscopy Cytology/Histology/Pathology/EM *******************************
Any suggestion on proper etchant procedure to use in order to do NiTi and NiTiCu Memory Shape Alloys SEM images? We have tried several polishing procedure (cutting speed, different clothes and a chemical etch with 50:50 mixture of HF acid and HNO3 acid) but no grain borders can be seen (but some pinning if you use acid for too long!) Thank You in advance, Edoardo
{!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN"} {HTML} {HEAD} {META HTTP-EQUIV=3D"Content-Type" CONTENT=3D"text/html; = charset=3Dus-ascii"} {META NAME=3D"Generator" CONTENT=3D"MS Exchange Server version = 5.5.1960.3"} {TITLE} SEM preparation of SMA {/TITLE} {/HEAD} {BODY}
{P} {FONT SIZE=3D2 FACE=3D"Courier New"} Any suggestion on proper etchant = procedure to use in order to do NiTi and NiTiCu Memory Shape Alloys SEM = images? {/FONT} {BR} {FONT SIZE=3D2 FACE=3D"Courier New"} We have tried several polishing = procedure (cutting speed, different clothes and a chemical etch with = 50:50 mixture of HF acid and {/FONT} {/P}
{P} {FONT SIZE=3D2 FACE=3D"Courier New"} HNO3 acid) but no grain borders = can be seen (but some pinning if you use acid for too long!) {/FONT} {BR} {FONT SIZE=3D2 FACE=3D"Courier New"} Thank You in advance, = Edoardo {/FONT} {/P}
I am looking for software/hardware that will help me count the number amount , as well as note the area of the particle, off a negative (print) image that I have produced using a TEM. I could do it by hand, but the time neccessary is not available. I know that there has to be something out there that will help me, and any help would be greatly appreiciated.
Joseph Montoya Physics Department New Mexico State University
I am unsure about your imaging goals, but can you use BSE channeling contrast instead of an agressive etch? I haven't consulted the phase diagrams, but it is often possible to see grains (orientation related contrast) if the Z differences don't produce a poor "signal to noise ratio". Noise in this case being any undesired image signal.
Any suggestion on proper etchant procedure to use in order to do NiTi and NiTiCu Memory Shape Alloys SEM images? We have tried several polishing procedure (cutting speed, different clothes and a chemical etch with 50:50 mixture of HF acid and
HNO3 acid) but no grain borders can be seen (but some pinning if you use acid for too long!) Thank You in advance, Edoardo
Does anyone out there know of a source of single crystal LiF substrates or other similar single crystal fluorides, chlorides, etc? If not, would you perhaps know of an entrepreneurial crystal grower who might be tempted? Thanks in advance.
Cheers John
John P. McCaffrey Institute for Microstructural Sciences National Research Council of Canada M-50 Montreal Rd. Ottawa, Ontario K1A 0R6 Canada
As a result of a recent trade, I have a nice clean LKB-Huxley Ultra Microtome - seems to be in good working condition and has the original accessory box with a variety of chucks and adapters. And a new - unused - EdgeCraft 2.6mm diamond knife. All of my work is LM, so I need to get this stuff sold or traded (hopefully in the greater Baltimore-DC area for the Huxley, since I don't want to crate it). Money would be nice (my wife thinks my self-supported research costs way too much), but also open to interesting trades for useful LM equipment - I am Leitz 170mm based, but open to other possibilities. Also would consider selling/trading the Huxley and the diamond knife as seperate items. Mainly, I need to get the Huxley out of my wife's laundry room and don't have room for it in my lab - OK, I know ultra microtomes in the laundry room is not a common problem, but we are a little strange. And, this is clearly not a commercial enterprise.
Geoff Avern wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } A very big hello to everyone that I haven't spoken to since leaving the } Microscopy List some 2 years ago. My new vocation leads me to } re-subscribing to ask you all a very big favour. } } ***Have you done any EM work (imaging or analytical) for archaeologists? } If so, would you be so kind as to drop me a line to let me know the aim } and nature of the work? Reasons follow; } } I left the Microscopy Labs of the Australian Museum to follow my wife's } work in Brussels, Belgium, where I'm doing a Ph.D. in Archaeology (in 3D } modelling). The undergraduate courses in our faculty do not cover many } scientific techniques, so my fellow students have badgered me into giving } a seminar on EM's and their applications in archaeology, and a demo on the } uni's SEM. I have plenty of examples from my work in zoology, but } obviously I need examples from archaeology. I have some references to } work with but I figure that the BEST references are the people on this } List. } } Please understand that my motivations are honest. My only use of your } information will be this once-off seminar for my fellow students. They } are undergrads who are not in a position to poach any research for their } own advantage. All contributors will be acknowledged. And I will be } personally very grateful for your help. } } Hoping to hear from you, } } Geoff Avern } Brussels, Belgium
Geoff One person you might want to try and chase down is Dr. Alan Walker. He used to operate an SEM at Johns Hopkins University in Baltimore, MD, but I don't believe he is there at the present time. I beleive it was the Lucy find in Africa that he was associated with and he might be able to give a lot of input into the archeological uses of the SEM. Hope this helps.
Ken Converse owner Quality Images third party SEM service
What you want to do is relatively straightforward. You do have to be careful that the area fraction that you measure for the particle will not be the volume fraction because you have to take account for the thickness of the sample in transmission. For relatively inexpensive solutions, you can use NIH image on a MAC or Photoshop with John Russ' Image Processing Toolkit Plug-ins for both Mac and PC. IPTK is about $250 and comes with a very good tutorial and a primer on stereology measurements. It will also work with other programs. The latest version has digital darkroom on it. I also think that his plug-ins work with NIH image, but I have never tried them with it. IPTK also goes very well with his book, The Image Processing handbook. Visit their web site: http://members.aol.com/ImagProcTK/index.htm
John Russ monitors the Listserver and he is very helpful to people with quantitative microscopy problems as he was with me just over the past couple of days. Thanks again, John!
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
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(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
---------- } From: Rudy To: microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
I am looking for software/hardware that will help me count the number amount , as well as note the area of the particle, off a negative (print) image that I have produced using a TEM. I could do it by hand, but the time neccessary is not available. I know that there has to be something out there that will help me, and any help would be greatly appreiciated.
Joseph Montoya Physics Department New Mexico State University
Hello,=20 Can somebody help me about the preparation of the Sato's Lead Citrate? = What's the chemicals and its proportion? Thank's in advance. M.Sc. Rinaldo Pires dos Santos Lab. of Plant Anatomy - Dept. of Botany - UFRGS Porto Alegre - RS - Brazil
Hello, Can somebody help me about the preparation of the Sato's Lead Citrate? What's the chemicals and its proportion? Thank's in advance. M.Sc. Rinaldo Pires dos Santos Lab. of Plant Anatomy - Dept. of Botany - UFRGS Porto Alegre - RS - Brazil
We did some work on materials investigation of a 300-year old Virgin Mary stone (Indo-Christian style). We picked up small pieces of samples from different positions of the stone, e.g., coating, and stone itself. The results are interesting. We analyed mineral constituents and chemical compositions and finally we were able to determine the most possible locality where the stone was produced.
The paper was published at MRS Proceedings: M.S. Barger and W.L. Gong, La Virgincita: Technical study of an Indo-Christian Statue, Mat. Res. Soc. Proc. Symp. 462, 381 (1997).
I would appreciate comments or opinions regarding this software. I saw the presentation and I was quite impressed. Howver before I can make any recommendation I would like to hear from users of this software especially those using the Confocal Imaging module and Cell Tracking.
My two cents worth is to throw that mental switch into your head in the analytical mode. As you know your EM, and my Pol Scope, do analysis as well as make WOW! pictures. To not assume, I will refer you to the Journal of Archaeological Sciences for many articles using the EM.
You have a "tool" that can analysis micro quantities, which archaeologist are not used to working with. You need to educate them, not what IS done, but the types of things can be done. Archaeological problems of the "what the .... is this" tend to come on a more or less dig by dig basis. Build an easy bridge for them to walk accross to your analystical tool. Problem solving is best done with the interest of the various are openly and freely discussed.
While not archaeology, don't forget conservation and restoration as you consider your course.
Good luck and Shalom from Jerusalem, Azriel
} } Brussels, Belgium } } Geoff } One person you might want to try and chase down is Dr. Alan } Walker. He used to operate an SEM at Johns Hopkins University in } Baltimore, MD, but I don't believe he is there at the present time. I } beleive it was the Lucy find in Africa that he was associated with and } he might be able to give a lot of input into the archeological uses of } the SEM. } Hope this helps. } } Ken Converse } owner } Quality Images } third party SEM service } } +-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+ Azriel Gorski azrielg-at-cc.huji.ac.il ICQ User ID No. - 1750739
CHOICE - The enchanted blade, with an edge that shapes lifetimes. Richard Bach RUNNING FROM SAFETY
A friend is someone who knows the song in your heart, and can sing it back to you when you have forgotten the words. +-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+
We run in-house courses on EDS analysis, WDS analysis, combined EDS / WDS analysis and Quantitative X-ray Mapping. These courses are all 'hands on' using ETEC and JEOL Microprobes. We offer these courses to all University and Industry Personnel.
Moran Scientific Pty Ltd P.O. Box 651 Goulburn NSW 2580 Australia Tel 02 4844 4234 (International, 61 2 48444234) Fax 02 4844 4291 (International, 61 2 48444291) Email {kmoran-at-goulburn.net.au} Web Page http://www.goulburn.net.au/~kmoran "Tell me and I'll forget, show me and I may remember, involve me and I'll understand" - an Old Chinese Proverb
Dear all, has anybody experience or can give me tips for the preparation/examination of liposomes ( {100nm diameter) for TEM or SEM . We want to analyse the homogeneity of suspensions and to estimate the single volume of the liposome particles? Unfortunately we don't have any unit for cryomicroscopical observations! Any suggestions are welcome... best regards, Bernward Bernward Laube University of Bielefeld Faculty of Biology Department Plant Morphology and Cell Ultrastructure Universit„tsstrasse 25 Germany 33615 Bielefeld phone: +49 521 1065592 fax: +49 521 1066039 e-mail: b.laube-at-biologie.uni-bielefeld.de http://www.uni-bielefeld.de/biologie/Pflanzenmorphologie
Hello: We are considering doing TEM to look at the microstructure around surface pits in molded stainless steel parts. We are trying to understand the source of these pits. So far SEM and OM have provided us with no relevant clues. The pits are barely visible by eye and they are only very few of them on a given part. We are thinking of doing tripod polishing , but I would appreciate any comments/suggestions on how one might best approach this.
Rinaldo, Here's the recipe for Sato's Triple Lead stain that we use:
0.5 gm Pb nitrate 0.5 gm Pb acetate 0.5 gm Pb citrate 1.0 gm Na citrate 41 ml boiled,distilled water
-Mix in covered tri-pour beaker for at least 2 hours (all day is better) -Add 9.0 ml 1N NaOH to clear(changes milky-white color to clear)
If you have any questions or comments, don't hesitate to contact me. Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W Wiggins, Supervisor 2/24/99 8:43:32 AM CRC-Electron Microscopy Lab. Ofc:704/355-1267 Carolinas Medical Center Fax:704/355-7648 P.O. Box 32861 Lab:704/355-7220 Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
I recall reading a few years ago a description of the Hilbert (spelling?) raster pattern generator consisting of interlocking H's in a fractal regression of scale. As I recall, the advantages touted for this raster were equivalent (on overage) probe deflection speeds in the x & y directions, a continuous path for the raster probe, and a low sensitivity to scan coil saturation at rapid scan rates.
I have an "opportunity" to replace my scan generator with a digital device, and would like to give the Hilbert a try. The problem is that I misplaced my copy of the article. Does anyone recall the source of this reference?
Thanks,
valdemar-at-fast.net or rwafu-at-bsco.com Valdemar Furdanowicz Homer Research Labs G-165 Bethlehem Steel Co. Bethlehem, PA 18016
Dear Rinaldo: Here is the protocol for Sato's Lead Citrate:
Anhydrous lead citrate Pb(C6H5O7)2 0.20 g Lead nitrate Pb(NO3) 0.15 g Lead acetate Pb(CH3COO)2.3H2O 0.15 g Sodium citrate Na3(C6H5O7).2H2O 1.00 g Distilled water 41.0 ml
The above reagents placed in a 50 ml voletric falsk were mixed well to make a yellowish milky solution. Then the solution was added 9.0 ml of 1 N NaOH and mixed well until the solution became transparent with light yellowish color. KEEP IT IN REFRGERATOR, IT COULD BE GOOD OVER ONE YEAR.
Here is some useful references: Sato, T: Electron Microsc, 17: 158, 1968 Watson, ML: J Biophys. Biochem Cytol, 4:727, 1958 *Hanaichi T, Sato T, Hoshino M and Mizuno N: Proc XIth Int Cong on Electron Microscopy, Kyoto, 1986 -- This is where "my" recipe came from. Good Luck.
Zhaojie Zhang ********************************* Zhaojie Zhang Department of Botany and Microbiology University of Oklahoma Norman, OK 73019 Phone: 405-325-6234 http://students.ou.edu/Z/Zhaojie.Zhang-1/ *********************************
Rinaldo Pires dos Santos wrote:
} Hello, } Can somebody help me about the preparation of the Sato's Lead Citrate? } What's the chemicals and its proportion? } Thank's in advance. } M.Sc. Rinaldo Pires dos Santos } Lab. of Plant Anatomy - Dept. of Botany - UFRGS } Porto Alegre - RS - Brazil
there are several software packages available that do exactly what you want: threshold the particle, analyze them and give you particle results such as area, max. radius, etc. In my opinion you should look for software that:
a) calculates all the parameters you need b) allows you to define your own parameters (for future extension) c) provides an upgrade path if you need more processing at a later time d) reads and writes standard files for data exchange e) provides features for particle separation (watershed or other) f) allows you to set up Regions of Interest (connected and non-connected) g) provides density measurements h) deals correctly with edge particles i) allows user defined classifications
Please check out our website for more information.
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com web: http://www.soft-imaging.com
******************************************************* Disclaimer: Soft Imaging System produces and sells image acquisition and processing systems. We therefore have a vested interest in some of the items mentioned above. *******************************************************
DATE: Tue, 23 Feb 1999 13:06:56 } From: Rudy {mojoseph-at-NMSU.Edu} To: microscopy-at-Sparc5.Microscopy.Com
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I am looking for software/hardware that will help me count the number amount , as well as note the area of the particle, off a negative (print) image that I have produced using a TEM. I could do it by hand, but the time neccessary is not available. I know that there has to be something out there that will help me, and any help would be greatly appreiciated.
Joseph Montoya Physics Department New Mexico State University
Sato lead citrate was published by T. Sato on the Journal of Electron Microscopy vol.17,No.2, 158-19, 1968.
The recipe is as follows:
Lead nitrate 1 g Lead acetate 1 g Lead citrate 1 g sodium citrate 2 g
in 82 ml of distilled water, then add 4% NaOH 18 ml. pH at 12.
There is another recipe in J. Electron Micro., 116, 133 (1976).=20
Best regards,
Ming
On Tue, 23 Feb 1999, Rinaldo Pires dos Santos wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America= =20 } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } =20 } =20 } Hello,=20 } Can somebody help me about the preparation of the Sato's Lead Citrate? } What's=A0 the chemicals and its proportion? } Thank's in advance. } M.Sc. Rinaldo Pires dos Santos } Lab. of Plant Anatomy - Dept. of Botany - UFRGS } Porto Alegre - RS - Brazil } =20 } =20
*********************************************** * Ming H. Chen, PhD * * Medicine/Dentistry Electron Microscopy Unit * * #1074B Dentistry Pharmacy Building * * University Of Alberta. * * Edmonton, Alberta, Canada T6G 2N8 *=20 * * * Visit My Page At: * * http://www.ualberta.ca/~mingchen * ***********************************************
Dear Rinaldo: Here are two references that may be helpful: 1) Takagi, I., etal, 1990. Penetration and stainability of Modified Sato's Lead Staining Solution. J. Electron Microsc. 39:67-68.
2) Hanaichi, T.,etal.. 1986. A Stable Lead by Modification of Sato's Method. J. Electron Microsc. 35:304-306.
The original Sato reference is J. Electron Microsc 17:158 (1968).
Hmmm.. I guess that's three. If you don't have access to these, contact me directly.
Don Gantz Boston Univ Med School gantz-at-med-biophd.bu.edu
On Tue, 23 Feb 1999, Rinaldo Pires dos Santos wrote:
} Date: Tue, 23 Feb 1999 21:14:06 -0300 } From: Rinaldo Pires dos Santos {rinaldop-at-botanica.ufrgs.br} } To: Microscopy-at-sparc5.microscopy.com } Subject: Sato Lead Citrate } =20 } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America= =20 } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } =20 } =20 } Hello,=20 } Can somebody help me about the preparation of the Sato's Lead Citrate? } What's=A0 the chemicals and its proportion? } Thank's in advance. } M.Sc. Rinaldo Pires dos Santos } Lab. of Plant Anatomy - Dept. of Botany - UFRGS } Porto Alegre - RS - Brazil } =20 There are several recipes for something called "Sato lead;" I don't know=20 what the original was. Here is the one we use:
1g lead nitrate 1g lead citrate 1g lead acetate 2g sodium citrate 82 ml distilled water
Shake 1 min. Sol'n will look milky. Then add 18 ml freshly prepared (from pellets) 4% NaOH (1N). Sol'n will=20 clear, except for large grains in bottom. Filter rapidly and store=20 sealed in dark bottle (plastic, not glass; can wrap with Al foil). Keep li= d=20 on. If white precipitate forms on top, remove stain from area away from=20 ppt. Can microfuge just before use to remove ppt. Lead carbonate ppt=20 on the grid looks like little "Pacmans" in the scope--very black circles=20 (100-200 nm +/-) with one side usually rough or indented. (Uranyl acetate= =20 precipitate is finer-grained--looks like pepper. Phosphate buffers can=20 also look like pepper, but grains are usually a little darker. UA=20 deposits are frequently ON subcellular structures--membranes, ribosomes,=20 etc; PO4 deposits are usually in the cytosol--where you would expect water.= )
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710=20 Ph: 919 684-3452 FAX: 919 684-8735
McCaffrey, John (IMS) wrote: } } Does anyone out there know of a source of single crystal LiF } substrates or other similar single crystal fluorides, chlorides, etc? } Dear John, I got mine from Ted Pella. I am not affiliated with them ex- cept as a customer. Yours, Bill
Dear Bernward Laube: A relatively simple method to evaluate liposomes is to fix with osmium tetroxide to make them more rigid and less likely to flatten (effectiveness will depend upon composition of liposome) and then negative stain them on a grid. If you want to discuss in detail, contact me directly. Don Gantz Boston Univ Med School gantz-at-med-biophd.bu.edu
I am searching for any information regarding electrochemical etching of {110} Iridium to form a very sharp tip suitable for FIM use. Any information or direction towards information would be appreciated. Thanks, Randy
Try negative staining with a 2% ammonium molybdate (aq). Take a coated grid (carbon, formvar, etc.), put a drop of liposomes on top of the grid for a minute. Draw off drop with with a piece of torn filter paper. Before the grid dries, add a drop of the ammonium molybdate. After a minute, draw off drop as before and allow the grid(s) to dry. Take it to the TEM.
The above procedure is only a starting point. The concentration, stain, and times can all be varied to achieve optimum results. You might check out some EM texts on negative staining for other ideas. Good luck.
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Dear all, has anybody experience or can give me tips for the preparation/examination of liposomes ( {100nm diameter) for TEM or SEM . We want to analyse the homogeneity of suspensions and to estimate the single volume of the liposome particles? Unfortunately we don't have any unit for cryomicroscopical observations! Any suggestions are welcome... best regards, Bernward Bernward Laube University of Bielefeld Faculty of Biology Department Plant Morphology and Cell Ultrastructure Universit?tsstrasse 25 Germany 33615 Bielefeld phone: +49 521 1065592 fax: +49 521 1066039 e-mail: b.laube-at-biologie.uni-bielefeld.de http://www.uni-bielefeld.de/biologie/Pflanzenmorphologie
(SMTP Gateway for microscopy-at-sparc5.microscopy.com); Wed, 24 Feb 1999 13:50:24 -0500 Message-Id: {199902241850.NAA24523-at-gateway.ppg.com} Received: by gateway.ppg.com (Protected-side Proxy Mail Agent-1); Wed, 24 Feb 1999 13:50:24 -0500 Randy Percival {rperciva-at-micrion.com}
John Hren's book on FIM has it. Send me a message at home to remind me and I will look it up for you. (SKAB-Walck-at-worldnet.att.net) Another option is to call Alan Melmed at John Hopkins-He'll know for sure. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
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---------- } From: Randy Percival To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
I am searching for any information regarding electrochemical etching of {110} Iridium to form a very sharp tip suitable for FIM use. Any information or direction towards information would be appreciated. Thanks, Randy
We have a manual metalographic grinding/polishing wheel. The sample being prepared is held in the hand as it is pressed against the rotating wheel. Our safety people have asked us to provide finger protection for this device. Does anyone have any solution/suggestion? Everett Ramer
Jordi, Polishing is only going to contaminate your pit with all sorts of junk. The best way is to microtome the pit either normal or preferably in cross section. A diamond knife would make this quite easy although we have done good work with glass but this is a slow process as the knife must be repositioned every few cuts. One hint is to keep your cutting area very small, like a fraction of a mm. Good Luck, Russ Gillmeister
-----Original Message----- } From: Marti, Jordi [mailto:Jordi.Marti-at-alliedsignal.com] Sent: Wednesday, February 24, 1999 8:43 AM To: Microscopy
Hello: We are considering doing TEM to look at the microstructure around surface pits in molded stainless steel parts. We are trying to understand the source of these pits. So far SEM and OM have provided us with no relevant clues. The pits are barely visible by eye and they are only very few of them on a given part. We are thinking of doing tripod polishing , but I would appreciate any comments/suggestions on how one might best approach this.
Thanks to all who responded to my problem. But I still have the problem. I got a new cylinder of Argon. I checked the hoses to make sure they were not clogged. I double checked the polarity. My vacuum is good. But when I=20 turn on my DSM-5 I get a blue arc around the=20 middle of the cathode. After 3 minutes, I shut the sputter down but I have no coating on my sample. I checked the inside of the DSM-5 and found no loose wires, and the fuses were good. Any other suggestions would be greatly appreciated.
George Lawton Chief Electron Microscopist Microscopy and Imaging Service Center UT Southwestern Medical Center at Dallas Phone: 214-648-7291 eMail: George.Lawton-at-email.swmed.edu
Have you tried SWLI (scanning white light interferometry) or AFM?
SWLI has XY resolution comparable to OM (since you can see these pits by naked eye, that should suffice) but can have Z resolution as fine as about 5 nm. I have a Xerox copy of an older article available on this technology if you are interested (Amer Lab, Nov 1994) or you can visit the web sites of several of the manufacturers: Zygo, Wyko (now part of Veeco) and Phase Shift Technology. This approach would not only allow you to image but measure parameters such as depth, profile immediately around the pit, etc.
Caveat: I have no financial interest in sales derived from this message.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 06:43 AM 2/24/99 -0700, Marti, Jordi wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Summer School on Computing in Electron Microscopy slotted for August 9-13, 1999 , Berkeley, California
(Berkeley, CA) The seventh annual Summer School on Computer-Interactive HRTEM Image Acquisition, Processing and Simulation will be held at the National Center for Electron Microscopy (NCEM), Lawrence Berkeley National Laboratory, University of California, Berkeley from August 9 through August 13, 1999.
The curriculum will focus on training participants in techniques of computer-assisted acquisition and interpretation of high-resolution electron microscope images, including remote-control microscopy. Participants will learn general principles and apply them to specific cases. Instruction on use of computer assistance to obtain images on NCEM microscopes will be followed by training in the use of specific application programs for image interpretation by image processing and simulation.
Participants who wish to apply newly acquired techniques to their own projects are encouraged to extend their visit at NCEM into the next week. Please note: this type of arrangement requires advance submission of a proposal. Projects may involve prepared specimens for microscopy, images and diffraction patterns for processing, or crystal and defect data for simulations. The fee of $375 will cover all materials, instruction, continental breakfast daily, two lunches and an evening reception. Deadline for applications is June 15, 1999. For more information and downloadable application materials contact:
What kind of resolution can be achieved when imaging noble metal particles in catalyst? The catalyst comprises binder and zeolite. I image the system using a STEM/ADF and have been able to see {1 nm particles on binder.
However, I have not been able to see the ultra-fine particles (5-10 --0__=Su6dP4Po6DV5U1roKslOtYgCgwxd5t1scAJybnVta3mV5BnNAUWoo8fA Content-type: text/plain; charset=iso-8859-1 Content-Disposition: inline Content-transfer-encoding: quoted-printable
=C5) in the zeolite. Has anyone been able to image 5-10=C5 metal particles in zeolites? I am trying to reconcile the difference between images (which don't sho= w {1nm particles on zeolites) and chemisorption technique which indicate that the metal is very highly dispersed.
Thank you, Sincerely, Mohan Kalyanaraman
Sr. Staff Material Scientist Catalyst Characterization Catalyst Technology Group Mobil Technology Company PO Box 480 Paulsboro, NJ 08066 609-224-3989 (ph#) 609-224-3608 (fax) =
A couple of weeks ago I posted a query, looking for a specific LM stain for mitochondria. There were several responses from people wanting to know the same thing so here are the results.
First of all, thanks to everyone who replied. Only some of you got a personal thankyou because I lost track of who I had thanked and who I hadn't.
If you remember, I had some tissue already fixed in glutaraldehyde but not processed further. Ideally I wanted a LM stain that would work on semithin resin sections and preferably be compatible with TEM processing.
The only practical method anyone suggested was to do what I do now: process the tissue as normal for TEM and stain semi-thins with toluidine blue at high pH. The only problem with this is that nearly everything stains with tol. blue, and you have to identify the mitochondria by the intensity of their staining. So it is not a specific stain, but it is easy to do and fits in with the TEM processing.
Other respondents suggested Janus Green, and Mitotracker from Molecular Probes, but both of these only stain mitochondria in living cells and my cells are already fixed. According to the Molecular Probes catalogue, Mitotracker is retained well in stained cells after fixation (but the cells need to be alive when they are stained).
The only other suggestions were to try the methods developed several decades ago. I had read all these before posting my query and rejected them because none of them use the type of aldehyde fixation we use today.
So that's it. No ideal methods, and I haven't the time to play around and develop my own. You know how it is.
Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department Faculty of Medicine & Health Sciences University of Auckland Private Bag 92019 Auckland New Zealand
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(1) Reflection Optical Microscopy in NOMARSKI (Differential Interference Contrast) mode. This has similar high resolution in the Z direction as SWLI (Barbara Foster reply), but it does have the disadvantage, that it only works for nearly flat surfaces: if your steel objects have curved surfaces then the next might be useful:
(2) for TEM it is very easy to make replicas of steel surfaces using cellulose acetate, followed by shadow / carboning the first stage replica. We have used this on all sorts of specimens. It is very easy if your surface is flat or even cylindrical (as long as the curvature is not too great) but for spherical surfaces you can either (i) replicate a square part with only a small solid angle - say from the N.Pole to 80^ north; or (ii) reliplicate a thin nearly cylindrical strip along a latitude or longitude.
There are books which discuss extraction replicas, which not only give you the surface but can pull off tiny inclusions for electron diffraction, etc.
On Wed, 24 Feb 1999, Marti, Jordi wrote:
} We are considering doing TEM to look at the microstructure around } surface pits in molded stainless steel parts. We are trying to understand } the source of these pits. So far SEM and OM have provided us with no } relevant clues. The pits are barely visible by eye and they are only very } few of them on a given part. We are thinking of doing tripod polishing , but } I would appreciate any comments/suggestions on how one might best approach } this.
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
************************************ This international conference will address the world-wide state-of-the-art in semiconductor microscopy. The scientific programme, which contains over 180 papers, has been finalized and is available at the conference Web site http://www.iop.org/Confs/MSM
Online registration is available also at the same site.
A G Cullis MSMXI Co-Chairman
**************** Prof Anthony G Cullis EEE Department University of Sheffield Mappin Street Sheffield S1 3JD, UK
Hmmm.... Then you may want to check/replace the target. Blue arc may indicate you have the correct power supply and vacuum. But you should make a double check. -cy TEMist, Batta Labs, Delaware
On Wed, 24 Feb 1999, George Lawton wrote: } Thanks to all who responded to my problem. } But I still have the problem. I got a new cylinder } of Argon. I checked the hoses to make sure they } were not clogged. I double checked } the polarity. My vacuum is good. But when I } turn on my DSM-5 I get a blue arc around the } middle of the cathode. After 3 minutes, I shut } the sputter down but I have no coating on my } sample. I checked the inside of the DSM-5 and } found no loose wires, and the fuses were good. } Any other suggestions would be greatly } appreciated. } } George Lawton } Chief Electron Microscopist } Microscopy and Imaging Service Center } UT Southwestern Medical Center at Dallas } Phone: 214-648-7291 } eMail: George.Lawton-at-email.swmed.edu
I did not see the original tread. There is a list of "mite experts" who did a lot of TEM, SEm on mites. Don Griffin in the UK, Enrico de Lillo in Italy Carin Kameric in SA just to name a few. These individuals will be more than happy to help, give references etc. We are active in South Africa as well. I did a bit in confocal and SEM.
Hope this does help.
Mr. S H Coetzee Electron Microscope Unit Private bag X3 Wits Johannesburg 2050 Tell: +27 11 716 2419 Fax : +27 11 339 3407 E-mail stephan-at-gecko.biol.wits.ac.za
Mohan Rice and Treacy were able to image an individual Pt atom in a zeolite using high angle annular dark field microscopy with a HB501. Imaging an individual atom on a catalyst support or zeolite is quite difficult because of the problem in differentiating whether the intensity is a result of tip noise or scatter from a higher atomic number atom. The smallest cluster in a zeolite that I have been able to image and then reimage to demonstrate that it is scattering is about 0.4nm. One also has to be careful so as not to agglomerate the metal because of the beam energy and structural collapse of the zeolite. Pennycook has shown some work with the HB603 that an individual Pt atom on alumina can be imaged. The key to performing this type of work is utilizing a very thin sample so that scattering from the catalyst support is minimized. Orienting along a zone axis is often helpful. Sample thickness may be the reason you can image clusters on the binder and not in the zeolite. On the other hand, all of the Pt may be on the binder and not in the zeolite. Don't always believe that the impregnation and subsequent finishing accomplished the desired metals location.
Steve Bradley UOP sabradle-at-uop.com (847) 391-3321
} -----Original Message----- } From: Mohan Kalyanaraman [SMTP:mohan_kalyanaraman-at-EMail.mobil.com] } Sent: Wednesday, February 24, 1999 3:57 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Imaging of ultra-fine metal particles } } Fellow Microscopists, } } What kind of resolution can be achieved when imaging noble metal particles } in catalyst? } The catalyst comprises binder and zeolite. I image the system using a } STEM/ADF and have been } able to see {1 nm particles on binder. } } However, I have not been able to see the ultra-fine particles (5-10 { { } File: ATT17711.txt } }
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Hi all, I have a new problem that I believe hinges on fixation at very low pH. The problem is this: I want to fix virus crystals that appear to be very sensitive to pH change...i.e. they dissolve when put into any solution having a pH above about 4.6. They are stable in a stabilization buffer (40% PEG MME 2000 and 10mM CaCl2 in 0.1M sodium acetate pH4.6).
We have been successful fixing virus crystals in the past by first fixing in 0.1M glutaraldahyde in a stabilization buffer at pH 6.0 (47% saturated ammonium sulfate, 0.1M MES) for 72 hrs. Then we fixed briefly in 0.1 Glut in 0.67M PO4 buffer (pH 6.8) and finally in 1% OsO4 in 0.67 PO4 buffer for 1 hr. However, this was all done at a much higher pH than the present crystals can tolerate.
We have tried fixing the crystals by first adding glutaraldehyde to the stabilization buffer to a final concentration of 0.1% and leaving the crystals in this for 72 hrs. The crystals are fine. If moved to fix in higher pH buffer, the crystals dissolve. Therefore I then rinsed with stabilization buffer and added aqueous OsO4 directly to the buffer to a final concentration of 1% OsO4. This was followed by washes with buffer. The crystals still seemed to be intact. Next was to try to dehydrate by gently and gradually adding ETOH to the buffer-crystal prep. As soon as I started adding the ETOH, which both diluted the stabilization buffer and affected the pH, the crystals started to dissolve.
I suspect that the fixatives did not crosslink at the low pH and that the crystals were still so unstable that they disintegrated in the dilute ETOH/buffer mix. It did not surprise me that the glut would not work at the low 4.6 pH but I did think the OsO4 would work. By the way, the crystals did not turn brown with the OsO4.
Does anyone know what is the minimum pH that 0.1% glutaraldehyde and 1% Osmium will crosslink? Has anyone else had a similar fixation problem and come up with a solution? These crystals are very difficult to make and I hate to keep dissolving them.
Looking forward eagerly to suggestions. Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
for {Microscopy-at-sparc5.microscopy.com} Paris Thu, 25 Feb 1999 15:01:09 +0100 (MET) Message-ID: {36D55860.979FBCD4-at-wanadoo.fr}
Materials for sale (well cared and under maintenance) : 1/- Ultramicrotome MT-1 /SORVALL-PORTER BLUMM (manual type), weight 12
Kg Price 8000FF or 1220 EUROS. 2/- Knife maker LKB Type 7801B, serial 2776, weight 20 Kg Price 8000FF or 1220 EUROS 3/- SEM coating unit: POLARON model E5100, without rotary pump, weight
25 Kg Price 12000FF or 1830 EUROS Sending expenses are not included Hoan Please contact us off line: e-mail: opea.hoan-at-wanadoo.fr OPEA lab. 114, rue de la Jarry 94300-VINCENNES (FRANCE) Phone: 33.1.4328.3496 Fax: 33.1.4328.0364
We have a manual metalographic grinding/polishing wheel. The sample being prepared is held in the hand as it is pressed against the rotating wheel. Our safety people have asked us to provide finger protection for this device. Does anyone have any solution/suggestion? Everett Ramer
Could you elaborate on how you would fix liposomes w/ Osmium tetroxide.= We have tried the negative stain approach with some success but have to li= ve with the obvious artifacts such as flattening. There are likely other artifacts caused by ionic or chemical changes of the stain. The chemic= al fixation sounds promising but I'm not sure how this could be done on a = liquid sample.
Joe Neilly Abbott Laboratories D-45M, AP31 200 Abbott Park Rd. Abbott Park, IL 60064-6202 voice: (847)-938-5024 fax: (847)-938-5027 e-mail: joe.neilly-at-abbott.com =
Position Open: Research Electron Microscopist, to start July, 1999. (http://www.burnham-inst.org).
Highly motivated, conscientious individuals, especially with experience in negative staining, frozen hydrated, or other high resolution biological specimen preparation techniques, are encouraged to apply. Qualifications include a BA/BS or equivalent experience, preferably in one of the biological or physical sciences, or in bioengineering. Knowledge of the operation of a high resolution TEM is required. An ability to work independently as well as in a small group environment is important. The candidate will not be solely responsible for microscope maintenance, but will assist with such, as well as with operating/testing/developing instrumentation (e.g., transmission electron microscopes, cryo-holders, freeze-etch machine, cryo-microtome). Previous experience with and aptitude for such activities is desirable, although some on-the-job training is possible. Additional duties include routine and publication-quality photographic work and general assistance in examining biological specimens for members of the group. Applications should include a statement of experience and goals plus three letters of reference. Salary is competitive and commensurate with experience. EOE. Send applications to: The Burnham Institute, 10901 N. Torrey Pines Rd., La Jolla, Ca 92037 Attn: Dr. Dorit Hanein c/o Sherri Marinovich Director of Human Resources or e-mail www.humanresources-at-burnham-inst.org. REFERENCE JOB CODE DH.
"Sequential treatment by phosphotungstic acid and uranyl acetate enhances the adherence of lipid membranes and membrane proteins to hydrophobic EM grids", A. N. Barnakov, Journal of Microscopy. Vol 175, Pt 2, August 1994, pp. 171-174.
I've not actually tried the above, just collected the paper last year for future reference.
Gib Ahlstrand
----------------------------------------------------------------------------- } Try negative staining with a 2% ammonium molybdate (aq). Take a coated grid (carbon, formvar, etc.), put a drop of liposomes on top of the grid for a minute. Draw off drop with with a piece of torn filter paper. Before the grid dries, add a drop of the ammonium molybdate. After a minute, draw off drop as before and allow the grid(s) to dry. Take it to the TEM.
} The above procedure is only a starting point. The concentration, stain, and times can all be varied to achieve optimum results. You might check out some EM texts on negative staining for other ideas. Good luck.
} Chuck Butterick Engineered Carbons, Inc.
______________________________ Reply Separator _________________________________ } Subject: TEM/SEM of liposomes Author: {"B.Laube-at-biologie.uni-bielefeld.de"-at-sparc5.microscopy.com} at
} } Dear all, has anybody experience or can give me tips for the preparation/examination of liposomes ( {100nm diameter) for TEM or SEM . We want to analyse the homogeneity of suspensions and to estimate the single volume of the liposome particles? Unfortunately we don't have any unit for cryomicroscopical observations! Any suggestions are welcome... best regards, Bernward } } Bernward Laube University of Bielefeld Faculty of Biology Department Plant Morphology and Cell Ultrastructure Universit?tsstrasse 25
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
Dear Everett, Grinding your fingers down is the first rite of passage for any Metallurgist worth his/her salt. No good metallographer has any feeling left in thumb or forefinger. Will you mess with tradition? Seriously, any glove thick enough to provide protection will hamper the fine control necessary to get a flat surface. The only thing I can think of is some sort of clamp or vise to hold the sample. I have never seen one, however. Now you know why automatic polishers are so popular. You wrote: } } We have a manual metalographic grinding/polishing wheel. The sample } being prepared is held in the hand as it is pressed against the rotating wheel. } Our safety people have asked us to provide finger protection for this device. } Does anyone have any solution/suggestion? } Everett Ramer } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
Dear Mohan, I have imaged the catalyst used by Petro Canada in my 200 kV TEM and seen the larger particles you mentioned at 40,000X, but I wasn't looking for the small ones and I no longer have the sample. Have you tried conventional TEM at 100 or 200 kV? The size you are looking for is difficult, since they are clusters of only a few atoms, so they have very little electron stopping power. The problem is not resolution, but contrast. Have you tried conventional darkfield? You wrote: } Fellow Microscopists, } } What kind of resolution can be achieved when imaging noble metal particles } in catalyst? } The catalyst comprises binder and zeolite. I image the system using a } STEM/ADF and have been } able to see {1 nm particles on binder. } } However, I have not been able to see the ultra-fine particles (5-10 } =C5) in } the zeolite. } Has anyone been able to image 5-10=C5 metal particles in zeolites? } I am trying to reconcile the difference between images (which don't show } {1nm particles on zeolites) and } chemisorption technique which indicate that the metal is very highly } dispersed. } } Thank you, } Sincerely, } Mohan Kalyanaraman Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
What is the crystal made of (what is the virus? is it in a matrix inside the cell? what is on the surface of the virus or crystal?). Maybe it's something that doesn't get cross-linked by either glut or Os. How about water sol resins to avoid organic solvents? What are the research questions you want to ans by sectioning the virus?
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
O.k., using Methylmethcrylate and Butyl methacrylate the instructions say " degas with nitrogen" does anyone know what that actually means? Does it mean simply bubbling N2 gas through the resin mixture? Any one have any specific instructions / recommendations?
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu
Widely used as a routine stain for ultrathin sections, lead solutions are frequently employed after uranyl staining to produce high contrast and stain many cellular and tissue components. Sato's method of lead staining(1967) is one of several variations of lead staining since its introduction by Watson(1958), among which are two methods by Karnovsky(1961), one by Millonig(1961), Reynolds (1963), Venable and Coggeshall(1965), and Fahmy(1967).
We have recently begun using a refinement of Sato's method introduced by Hanaichi et al.(1986) to produce a long-term-storage lead solution that seems to give virtually no -CO3 precipitate and claims to be good for one year at room temperature. We'll see.
Indicated references: - Karnovsky MJ.(1961), Simple methods for 'staining with lead' at high pH in electron microscopy, J.biochem.biophys. Cytol.11,729.
- Millonig G.(1961). A modified procedure for lead staining of thin sections, J.biophys.biochem Cytol 11,736.
- Reynolds ES.(1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.J.Cell Biol.17,208.
- Venable JH, and Coggeshall R.(1965). A simplified lead citrate stain for use in electron microscopy. J.Cell Biol.25,407.
- Fahmy A.(1967). An extemporaneous lead citrate stain for electron microscopy, Proc. 25th Ann. Conf. EMSA,p.148.
- Sato T.(1967). A modified method for lead staining of thin sections.J. Electron Microsc., 16:133.
- Hanaichi T.,et al.,(1986). A stable lead by modification of Sato's method. J.Electron Microsc.,35:304.
General references: - Staining Methods for Sectioned Material, Lewis PR;Knight DP, Practical Methods in Electron Microscopy, Ed:Glauert AM.
- Principles and Techniques of Electron Microscopy,Biological Applications,3rd ed.,Hayat MA.
- Electron Microscopy, Principles and Techniques for Biologists, Bozzola JJ,Russell LD.
- Technical Tips II, Electron Microscopy Sciences
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W Wiggins, Supervisor 2/25/99 2:44:49 PM CRC-Electron Microscopy Lab. Ofc:704/355-1267 Carolinas Medical Center Fax:704/355-7648 P.O. Box 32861 Lab:704/355-7220 Charlotte,NC 28232-2861 USA Eml:wwiggins-at-carolinas.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
I need some advice about sample prep for TEM. I have several amorphous silicon thin films that have been deposited on glass substrates from which I would like to make samples for TEM. The films are ~200 A thick, and it is important that they not be folded over or significantly thinned during the preparation. I would generally prefer a chemical process, as I worry that mechanical processes or ion milling could significantly change the microstructure of the films.
I have been told that it is possible to prepare the samples I want to first scoring the surface of the film, then immersing it and the substrate in a weak solution of HF. A little gentle scraping and the film is supposed to float free of the substrate and I can then pick it up on a copper mesh grid. Does anyone have any experience with this technique? Any advice on how weak a "weak" solution might be, or how long I need to let the film+substrate soak before scraping?
I would also be very glad to hear of any alternative means to prepare the samples I need that don't involve HF.
Thanks,
Paul Voyles
Paul Voyles (609) 951-2627 voyles-at-research.nj.nec.com NEC Research Institute, 4 Independence Way, Princeton, NJ 08540
I need to evaporate nickel in my Balzers BAF 301 Freeze Etcher. Everything is ready to go except I can't find what the density of nickel is in grams/cc so that I can calibrate my Quartz Crystal monitor. No, this information is not in the Merck Index, a disappointment indeed! Any help would be appreciated.
Richard: I do this by attaching a glass pasteur pipet to a rubber tube on a N2 tank and very slowly (barely perceptible flow against my cheek) flow the gas. I immerse the tip into the resin mix (in the fume hood) and bubble it for about 5-10 min. It is worth the effort - we have done the expt. Tom
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211 (573)-882-4712 (voice) (573)-882-0123 (fax)
Hi all, I have recently finished my third year project in electron microscopic observations of the entry and exit of influenza virus. In it I used Thermanox coverslips to culture the cells on, one of the problems that I had was delamination of the coverslip from the resin (Spurrs) and wondered if this had any thing to do with the different densities of the Thermanox and resin. If anyone knows their densities I would be most grateful.
I need to purchase a liquid nitrogen filling device for the Reichert KF-80. This consists of the 35 liter dewar and the filling head that goes into the dewar. I have contacted Leica/Reichert and they are unable to supply me with the unit because I needed the older model version. The new one available does not have the same circuitry and therefore will not be not compatible.
This dewar and filling head are of the same vintage of the F-C through D series of the cryo attachment for their ultramicrotome. So if any of you purchased the cryo attachment for your Reichert Ultramicrotome and are no longer doing cryo-microscopy and would want to part with at least half of the equipment, please get in touch with me.
Thank you very much.
Margaret E. Bisher
NEC Research Institute 4 Independence Way Princeton, NJ 08540. Tel.: (609) 951-2629 Fax: (609) 951-2496 e-mail: peggy-at-research.nj.nec.com
We are looking for a used, but late model "research grade" SEM. A large chamber is imperative. Chamber porting for EDS, WDS, and BSE is also required. The system must be configured to provide a clean, high vacuum (heavy element carbides & similar work). VP systems will certainly qualify and are desirable if the clean high vacuum criteria can also be met. Digital (=} 1024x1024) or analog is OK. I do prefer manual/knobs over automatic/windows mode. Software may set things up correctly on the average, but we don't do average work. Specimens are metallics/oxides/carbides etc., elements range from boron through uranium in almost any possible combination.
For a point of reference, We currently have an Etec Autoscan which has been modified to use a mag-lev turbo pump. It is also fitted with a windowless EDS detector, Microspec 2A WDS, and GW Electronics BSE. Although the Etec is a 70s vintage SEM, it is an excellent instrument. It only falls short in the realm of very low kV / high resolution imaging, high resolution at hefty WDS beam currents (somewhat of a conflict) and chamber size.
Woody White McDermott Technology, Inc. Lynchburg Research Center P.O. Box 11165 Lynchburg, VA 24506-11165
} } We have a manual metalographic grinding/polishing wheel. The sample } } being prepared is held in the hand as it is pressed against the rotatin= g wheel. } } Our safety people have asked us to provide finger protection for this device. } } Does anyone have any solution/suggestion? } } Everett Ramer
Everett, =
Mary Mager's response, while funny, is actually quite accurate. There ar= e not too many ways to protect fingers while properly holding a small (1-1/4", typically) =
sample for grinding/polishing. =
I preface my next remarks by pointing out that I work for a manufacturer = of
metallographic equipment and consumables, and therefore have a financial interest in solving your problem:
We at BUEHLER, do offer a simple grinding fixture which might help. This=
fixture is a squat, stainless steel, hollow cylinder with a carbide ring around it's base. The sample is clamped within another hollow cylinder seated within the first. =
The two cylinders are threaded, so that the inner can be raised or lowere= d =
with respect to the carbide 'stop' of the outer. Engraved markings allow= =
material removal in increments as fine as 20microns. While this is not actually =
finger protection, per se, it will allow you to grasp something larger so=
that your =
fingers are not in such close proximity to the grinding wheel. We also offer =
a motor system which allows the fixture to rotate, in place, on the wheel=
My manual for our Blazers quartz crystal film thickness monitor QSG 301 says the density of nickel is 8.9
Aloha, Tina
} I need to evaporate nickel in my Balzers BAF 301 Freeze Etcher. Everything } is ready to go except I can't find what the density of nickel is in grams/cc } so that I can calibrate my Quartz Crystal monitor. No, this information is } not in the Merck Index, a disappointment indeed! Any help would be } appreciated.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
We have such a device, but are not willing to part with it. The reason I replied is that I can assure you that you can still use your KF80. The pump unit sometimes drives me nuts, so when I plunge freeze I merely fill the unit by hand. Take care to cover the cryogen tube, then pour LN2 in the working area with a small Dewar. I've done this with a blown fuse, but if your electronics work, it's easy to keep an eye on the LN2 level on the front panel. I find I have to add LN2 after every plunge (level goes down one light), but it just becomes part of the rhythm of freezing. Freeze, remove sample, cover tube, pour in nitrogen, uncover tube, freeze.
I have not tried this with impact freezing ("slamming").
Good luck!
Tina
} I need to purchase a liquid nitrogen filling device for the } Reichert KF-80. This consists of the 35 liter dewar and the filling head } that goes into the dewar. I have contacted Leica/Reichert and they are } unable to supply me with the unit because I needed the older model version. } The new one available does not have the same circuitry and therefore will } not be not compatible. } } This dewar and filling head are of the same vintage of the F-C } through D series of the cryo attachment for their ultramicrotome. So if any } of you purchased the cryo attachment for your Reichert Ultramicrotome and } are no } longer doing cryo-microscopy and would want to part with at least half of } the equipment, please get in touch } with me.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
If you know of anyone that may be interested in (and suited for) the "exciting world of industrial research" please pass them the following job posting. The position has been advertised at a technical level but we now have the opportunity to expand the position to a research scientist (Ph.D.) position. We have just installed a state-of -the art FEI XL830 DualBeam FIB and I am looking for someone to be the "FIB expert".
I am looking for a person with extensive focused ion beam (FIB) experience and preferably some transmission electron microscopy (TEM) experience to fill a position in the Materials Analysis and Characterization Group at the IBM Almaden Research Center. Much of the work will involve the operation of a dual beam FIB in collaboration with other scientists in the study of small structures, thin films, and magnetic structures. Areas of application include photoresists, magnetic thin films, and tips for atomic force microscopes. The scientist is expected to take responsibility for the operation of the FIB, assist in the training of other users, and collaborate with a wide variety of applications of the that device. In addition, the scientist will apply TEM techniques to related problems. Experience with FIB instruments and TEM is extremely important.
Experience with TEM sample prep - especially tripod polishing and micromanipulation, as well as TEM and SEM would be a plus.
IBM is an Equal Opportunity Employer, Women and Minorities are encouraged to apply.
Please include the names of three references with your resume.
Please contact me directly. Submit your resume to:
Dr. Philip M. Rice K19/D1 IBM Almaden Research Center 650 Harry Road San Jose, CA 95120-6099
Dear Listers, Just a question about the MSA standard for x-ray spectrum file format: do any of you use it and under what circumstances? I am looking to upgrade my old x-ray analyser computer soon and want to know whether this standard is ever used and when. Please reply to me off-list and I will summarize to the list if I get any results.
Thank you all, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
The density of nickel is 8.902 g/cc according to the American Society for Metals, Metal Handbook.
Mark Robertson National Research Council Integrated Manufacturing Technology Institute Vancouver, B.C. Canada Phone (604) 221-3073 Fax (604) 221-3088 mark.robertson-at-nrc.ca http://www.nrc.ca/imti/vanc_e.html
} -----Original Message----- } From: Grazul, John [SMTP:Grazul-at-nel-exchange.Rutgers.EDU] } Sent: Thursday, February 25, 1999 12:46 PM } To: 'Microscopy-at-sparc5.microscopy.com' } Subject: density of nickel for evaporation } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Evaporators All, } } I need to evaporate nickel in my Balzers BAF 301 Freeze Etcher. } Everything } is ready to go except I can't find what the density of nickel is in } grams/cc } so that I can calibrate my Quartz Crystal monitor. No, this information } is } not in the Merck Index, a disappointment indeed! Any help would be } appreciated. } } TIA, } }
In a message dated 99-02-25 14:56:29 EST, edelmare-at-casmail.muohio.edu-at-sparc5.microscopy.com writes:
{ { O.k., using Methylmethcrylate and Butyl methacrylate the instructions say " degas with nitrogen" does anyone know what that actually means? Does it mean simply bubbling N2 gas through the resin mixture? Any one have any specific instructions / recommendations? } }
Hi Richard,
Yes, this is correct. Simply bubble dry nitrogen gas at a *very low* flow rate through a pipette that's immersed in the mixture. Regarding how long to do this, we used to bubble with nitrogen for 15 minutes as a matter of routine. If the mixture contains benzoyl peroxide or a similar granular or dry catalyst, just bubble until the catalyst is completely dissolved.
Bob ****************************** Robert (Bob) Chiovetti, Ph.D. President Microimaging Technologies, Inc. Tucson, Arizona USA Tel./Fax (520) 546-4986 rchiovetti-at-aol.com Manufacturers' Representatives Systems Integrators Analog & Digital Imaging Systems Clinical & Research Microscopy Cytology/Histology/Pathology/EM *******************************
Huge thanks to you all - your responses to my query on EM & Archaeology has given me a stack of leads to chase up for my seminar. I will be contacting many of you privately in the course of the next week or so - s= o expect a call!
Geoff Avern Universit=E9 Libre de Bruxelles
P.S. It was nice to get responses from familiar names - it made me realise that I'm missing working in EM more than I thought!
Hi Paul, I looked at a similar sample recently, although in my case the Si was laser annealed and was crystalline. Here is the protocol I used. I don't think you will be able to pick up the Si from the etch in your case as the layer is too thin.
1) Grind the sample to a thickness of ~100 um by mounting a ~1 cm square piece face down on a glass slide using thermoplastic wax. By placing grade zero cover slips (~120 um thick) on either side of the sample you don't have to worry about grinding the sample away.
(By the way, I think that hand protection whilst using a grinding wheel is just not necessary. It is not a chainsaw! I have never hurt myself when grinding a sample which is properly mounted on a proper (large enough) stub - maybe you could put a big flange on your stubs to keep your decidedly overzealous safety officer happy.)
2) Take the sample off the glass slide and put it in a small amount of conc. HF (48%). This will dissolve the glass completely in less than half an hour. The Si film will probably break up into many very small fragments (biggest being a few hundred um across).
3) Dilute the HF at least 20 times with DI water.
4) Filter the solution (preferably using a smooth filter paper).
5) Wash out any remaining HF from the filter paper with DI water.
6) Let the filter dry.
7) Take a 200 mesh TEM grid and 5-minute epoxy. Make up a small blob of epoxy and dunk the grid in it. Put the grid between two pieces of filter paper and remove most of the glue. Do this two or three times more so that you have a grid coated with a very thin layer of tacky glue.
8) Holding the grid in tweezers and working under a low mag optical microscope, pick up Si fragments by touching them with the grid.
9) If you're really in a hurry, you can stick the grid on a hot plate at ~170C for 30 secs (after the epoxy has cured) to outgas it.
It took me less than 90 minutes from getting the sample to having it in the microscope.
} I need some advice about sample prep for TEM. I have several amorphous } silicon thin films that have been deposited on glass substrates from which } I would like to make samples for TEM. The films are ~200 A thick, and it } is important that they not be folded over or significantly thinned during } the preparation. I would generally prefer a chemical process, as I worry } that mechanical processes or ion milling could significantly change the } microstructure of the films. } } I have been told that it is possible to prepare the samples I want to } first scoring the surface of the film, then immersing it and the substrate } in a weak solution of HF. A little gentle scraping and the film is } supposed to float free of the substrate and I can then pick it up on a } copper mesh grid. Does anyone have any experience with this technique? } Any advice on how weak a "weak" solution might be, or how long I need to } let the film+substrate soak before scraping? } } I would also be very glad to hear of any alternative means to prepare } the samples I need that don't involve HF. } } } Thanks, } } Paul Voyles } } Paul Voyles (609) 951-2627 voyles-at-research.nj.nec.com } NEC Research Institute, 4 Independence Way, Princeton, NJ 08540
Its not clear to me what your trying to measure here, but if it is the surface profile (ie the physical form, depth etc.) of these corrosion pits, then how about conoscopic holography. Uniscan manufacture a non- contact optical surface profiler which can use a conoscopic head in order to measure surface profiles on the sub - micronic scale, without touching the surface (and down holes too! - not too many optical techniques can do this).
Much less involved than TEM - or SEM for that matter.
If it the dynamics of pitting you want to measure then we also manufacture various scanning probe electrochemistry systems (SRET,SVET,LEIS) which will measure the localised pitting currents, and hence the corrosion rate, dynamically whilst the thing is pitting.
Let me know if you want more details - or see www.uniscan.co.uk.
Good luck.
Graham Johnson.
In message {Pine.GSO.3.96.990225093315.6816A- 100000-at-suma3.reading.ac.uk} , Robert H. Olley {R.H.Olley-at-reading.ac.uk} writes } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
-- Graham R. Johnson PhD. CPhys. MInstP. ________________________________________________________________________
I asked my metalsmith wife this question, as she's often grinding little bits of metal and such, and has a fondness for her fingers. Her recommendations are: 1) *Do not* wear gloves! They are a safety hazard and can be caught in the grinding wheel. Then you won't have to worry about your fingers. 2) Try a dop stick. This is a wooden dowel with sealing wax on the end in which the sample is held. Krazy glue can also be used to attach the sample. These sticks allow fine control. Gem facetters use dop sticks, for instance. 3) If you prefer to hold the pieces in your fingers, use leather finger tips. They cover just the tips of the fingers and since they come off easily are no hazard. They may not allow you the fine control you want.
Check your local college's art department or any art supply or lapidary store for these supplies (dop sticks are just dowels, so get them at a cheap hardware store--the wax may have to be gotten elsewhere).
Phil
} We have a manual metalographic grinding/polishing wheel. The sample } being prepared is held in the hand as it is pressed against the rotating wheel. } Our safety people have asked us to provide finger protection for this device. } Does anyone have any solution/suggestion? } Everett Ramer
****be famous! send in a tech tip or question*** Philip Oshel Technical Editor, Microscopy Today PO Box 620068 Middleton, WI 53562 Voice: (608) 833-2885 Fax: (608) 836-1969 (please make sure my name is on any fax) oshel-at-terracom.net
I want to thank everyone for all the responses and suggestions I received on looking at surface pits in molded stainless steel parts.
The suggestions ranged from doing microtomy to FIB , SEM (and EDS) as well as replication . Polishing seems to be the less desirable because it could introduce artifacts and contaminants that would complicate matters.
Currently we have two reconditioned microscopes available for purchase and can provide service contracts. Please contact me at this address for more details.
Mark W. Rigler, Ph.D. Vice President MAS, Inc. Suwanee, GA
Holy Cow! Talk of your rapid and accurate responce! I guess I either have to buy the CRC, or take up Liechtensteinian {so that I can translate my manuals}. It does turn out that the Quartz Crystal control manual is the one manual that I do not have; the others are in German, at least the pictures are really cool.
Polishing (crossections) can give you very good results if you apply low viscosity epoxy on surface in advance - to protect edge and debris in pits. You can get good sample for SEM/EDS investigation - use diamond and do not use soft polishing wheels, or you can prepare thin sections for STEM/EDS - do not use electrolytic thinning, ion mill is better for samples as yours. I do not believe you will get sufficient results for your investigation using TEM.
Vladimir Dusevich Electron Microscope Lab Manager UMKC
On Fri, 26 Feb 1999, Marti, Jordi wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I want to thank everyone for all the responses and suggestions I received } on looking at surface pits in molded stainless steel parts. } } The suggestions ranged from doing microtomy to FIB , SEM (and EDS) as } well as replication . Polishing seems to be the less desirable because it } could introduce artifacts and contaminants that would complicate matters. } } } } Thanks again, } } Jordi Marti } } }
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I'm sorry for trouble, but I have missed the discussion about = free(share)ware software for frame averaging a month ago and would like = to ask someone who initiated the discussion to send me a summary. Net is increadibly slow at my place and I can't search the archive. TIA Andrew
Dear Woody, I saw a notice last week in this listserver for a Hitachi S-570 for sale. This is what I use to do my EDX, WDX (Microspec WD-3PC) and BSE. It is very reliable, has incredible beam stability, high resolution and very high beam current capabilities. With an optioal low-kV gun it can do great low kV work. I bought a new S-570 years ago to replace my old Etec Autoscan. I recently purchased a used S-570 for less than $10,000 for EBSP work. The S-2500 is the later model of this with a larger chamber and it is also very good. You wrote: } We are looking for a used, but late model "research grade" SEM. A } large chamber is imperative. Chamber porting for EDS, WDS, and BSE } is also required. The system must be configured to provide a } clean, high vacuum (heavy element carbides & similar work). VP } systems will certainly qualify and are desirable if the clean high } vacuum criteria can also be met. Digital (=} 1024x1024) or analog } is OK. I do prefer manual/knobs over automatic/windows mode. } Software may set things up correctly on the average, but we don't } do average work. Specimens are metallics/oxides/carbides etc., } elements range from boron through uranium in almost any possible } combination. } } } For a point of reference, We currently have an Etec Autoscan which } has been modified to use a mag-lev turbo pump. It is also fitted } with a windowless EDS detector, Microspec 2A WDS, and GW } Electronics BSE. Although the Etec is a 70s vintage SEM, it is an } excellent instrument. It only falls short in the realm of very low } kV / high resolution imaging, high resolution at hefty WDS beam } currents (somewhat of a conflict) and chamber size. } } Woody White } McDermott Technology, Inc. } Lynchburg Research Center } P.O. Box 11165 } Lynchburg, VA 24506-11165 Good luck, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 fax: 604-822-3619 e-mail: mager-at-interchg.ubc.ca
I have a Kevex 7700 XRF unit and have found out recently that the = printer interface card isn't working. Does anyone know where I can get a = spare other than Kevex (Kevex is on the expensive side). Thanks.
______________________ Roberto Garcia Senior Analyst, Metallography North Carolina State University Analytical Instrumentation Facility Box 7531, Room 303 EGRC Raleigh, NC 27695-7531 rgarcia-at-unity.ncsu.com http://spm.aif.ncsu.edu/aif
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD} {META content=3Dtext/html;charset=3Diso-8859-1 = http-equiv=3DContent-Type} {STYLE} {/STYLE}
{META content=3D'"MSHTML 5.00.0910.1309"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {FONT size=3D3} I have a Kevex 7700 XRF unit and have found out = recently that=20 the printer interface card isn't working. Does anyone know where I can = get a=20 spare other than Kevex (Kevex is on the expensive side). = Thanks. {/FONT} {/DIV} {DIV} {/DIV} {DIV} ______________________ {BR} Roberto Garcia {BR} Senior Analyst,=20 Metallography {BR} North Carolina State University {BR} Analytical = Instrumentation=20 Facility {BR} Box 7531, Room 303 EGRC {BR} Raleigh, NC 27695-7531 {BR} {A=20 href=3D"mailto:rgarcia-at-unity.ncsu.com"} rgarcia-at-unity.ncsu.com {/A} {BR} {A=20 href=3D"http://spm.aif.ncsu.edu/aif"} http://spm.aif.ncsu.edu/aif {/A} {/DIV= } {/BODY} {/HTML}
1. EELS imaging was recommended for catalyst particles; 2. You need to worry about contrast more than resolution; 3. Finer the probe size in the STEM, the resolution is going to be better 4. Difficulty in seein g these due to the fact they are only a few atom clusters; may be try to compare contrast between zeolite with Pt atoms vs. no Pt atoms 5. Zeolites are prone to damage in beam - computer acquisition helpful to lowering current and acquiring metal particle images 6. Tracey and Rice have imaged indivudual Pt particles in zeolite L; agglomeration of metal under beam due to zeolite structure damage is a problem; thin samples are better 7. Energy filtered TEM may work, but try STM (scanning tunneling microscope) 8. Size of probe and Thickness of zeolite important - affects signal to noise ratio 9. Thin sectioning will help - microtoming
Various people have been able to image single atoms on alumina.
Thanks to all those that responded.
As always, it is great that we have a forum to share the collective experience. I have a detailed summary of postings that I can mail it to anyone who is interested.
Mohan Kalyanaraman
Sr. Staff Material Scientist Catalyst Characterization Catalyst Technology Group Mobil Technology Company PO Box 480 Paulsboro, NJ 08066 609-224-3989 (ph#) 609-224-3608 (fax)
I'm in search of a TIFF image that was taken using an Philips XL series SEM that is running with a 50Hz mains. The image detail is unimportant as is linescan time. It just needs to be taken with the standard XL TIFF export function. This is for an internal project that we are doing. You can e-mail it to me at the below address (watch doing a reply as it might go to the list server and we don't want that).
Thanks Scott ----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
I was under the impression that this server was NOT the place for posting items for sale. If that is the case then ignore this message. If I have been wrong or the rules have changed then fellow readers I have some equipment that is available:
edelmare-at-casmail.muohio.edu-at-sparc5.microscopy.com wrote: } } O.k., using Methylmethcrylate and Butyl methacrylate the instructions say " degas with } nitrogen" does anyone know what that actually means? Does it mean simply bubbling N2 } gas through the resin mixture? Any one have any specific instructions / } recommendations? } Dear Richard, Bubbling N2 through the resin will displace disolved O2, so that would be an indicated procedure if you don't want O2 in the resin. After this, however, I'd pull a vacuum on the mix (using house vac) to get rid of the N2. The latter will prevent bubble formation during polymerization. Yours, Bill Tivol
I will offer this initial reply on-list, then suggest that we take the discussion off-line.
We have used a Pixera for a couple years now and have no problems with it. Software updates did much to improve the quality of the viewfinder.
Are you sure that you rebooted after installing the Pixera drivers and software? (That may require a full reboot rather than just a suspend/resume thing like many new PCs support.) We recently moved our Pixera to a Pentium II 450 from a Pentium 200, and I think I got the "can not initialize camera" message after I loaded the software but before I rebooted. Unfortunately, I cannot tell you about the NT behavior. Although I had NT on our Pentium 200, I never tried installing the Pixera software for NT. Maybe I can.
If you still need help, perhaps you can reply directly. I also have a contact at Pixera if they need to step in.
At 02:08 PM 2/23/99 +0100, you wrote: } Dear Colleagues, } } We recently purchased Pixera Professional digital camera (for optical } microscopy). Nice little thing, but: } } We want to have the camera on PC (Pentium II, 333MHz, 128MB RAM) with Win NT } 4.0 operating system. } We successfully installed the NT driver Pixdrv.drv and Pixera Visual } Communication Suite (from a CD). After starting Studio Viewfinder (a } software for picture acquisition), there was just fine b/w raster (noise) } instead of picture. Using other software and Twain interface led us to the } same disappointment. } We repeated experiments with both service packs for NT 4.0, namely 3 and 4. } } We also tried to install the same camera on another computer (Pentium MMX } 200MHz, 64MB RAM ), running on Win 98. New hardware was installed without a } problem, but when opening the Pixera VC Suite and running Viewfinder } program a message "Can not initialize the camera" appeared. } } I would be very grateful for any suggestion. } ------------------------------ } Dr. Goran Drazic } J. Stefan Institute } Jamova 39 } SI-1001 Ljubljana } Slovenia
I like to have some way to pull the x-ray spectra from our EDS for use elsewhere. Once in a while we get an adventurous grad student who thinks they want a spectrum in an Excel file so they can do their own processing. Other times, we want to overlay spectra from our two, disimilar x-ray systems. In those cases, we want to be able to export the data to something that a spreadsheet can read. The exact format doesn't matter that much to me. It could be MSA format or plain text, preferably in a single column or in two columns of energy and intensity.
MSA format is one answer to the problem, but I would probably relax the constraints to simply something readable by a spreadsheet.
Warren
At 02:39 PM 2/25/99 -0800, you wrote: } } Dear Listers, } Just a question about the MSA standard for x-ray spectrum file format: do } any of you use it and under what circumstances? I am looking to upgrade my } old x-ray analyser computer soon and want to know whether this standard is } ever used and when. Please reply to me off-list and I will summarize to the } list if I get any results. } } Thank you all, } Mary } } Mary Mager } Electron Microscopist } Metals and Materials Engineering } University of British Columbia
Let me first describe the reason for this request. As a professor in a Faculty of Engineering, I co-ordinate the teaching of a basic course in Materials Engineering. This course is annually given to nearly one thousand students. Presently, I am preparing an interactive CD-ROM which will be a complement for a book already published (in French) and used as a reference manual in this course. Today, there is the opportunity to include video-clips, short movies, animations, sounds on a media such as CD-ROM with the pedagogical benefits of such tools. In Materials Science, some topics such as the glide of dislocations on a slip plane, cross-slip of dislocations, pile-ups of dislocations along grain boundaries, Frank-Read sources, Orowan’s mechanism are difficult to teach with only the help of traditional 2D figures in a book. Video-clips with sound track and movies can now easily be included on a CD-ROM and will surely help the students to better understand these topics.
So, my request is the following: I am looking for videos or movies showing the movement of dislocations or any of the dislocation mechanisms described above as directly seen in thin foils observed in TEM. If any of you has already on his shelves such video or movies (or knows somebody who has this type of audiovisuals), please get in touch directly with me at this e-mail address jbailon-at-email.phys.polymtl.ca ). Arrangements will be made for the duplication of the documents and for their copyright. Obviously, due credits will be given in the CD-ROM for the authors of these documents. Many thanks in advance.
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD} {META HTTP-EQUIV=3D"Content-Type" CONTENT=3D"text/html; = charset=3Diso-8859-1"}
{META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {P align=3Djustify} {FONT size=3D2} Let me first describe the reason for = this request.=20 As a professor in a Faculty of Engineering, I co-ordinate the teaching = of a=20 basic course in Materials Engineering. This course is annually given to = nearly=20 one thousand students. Presently, I am preparing an interactive CD-ROM = which=20 will be a complement for a book already published (in French) and used = as a=20 reference manual in this course. Today, there is the opportunity to = include=20 video-clips, short movies, animations, sounds on a media such as CD-ROM = with the=20 pedagogical benefits of such tools. In Materials Science, some topics = such as=20 the glide of dislocations on a slip plane, cross-slip of dislocations, = pile-ups=20 of dislocations along grain boundaries, Frank-Read sources, = Orowan’s=20 mechanism are difficult to teach with only the help of traditional 2D = figures in=20 a book. Video-clips with sound track and movies can now easily be = included on a=20 CD-ROM and will surely help the students to better understand these=20 topics. {/FONT} {/P} {P} {FONT size=3D2} So, my request is the following: I am looking for = videos or=20 movies showing the movement of dislocations or any of the dislocation = mechanisms=20 described above as directly seen in thin foils observed in TEM. If any = of you=20 has already on his shelves such video or movies (or knows somebody who = has this=20 type of audiovisuals), please get in touch directly with me at this = e-mail=20 address ( {A=20 href=3D"mailto:jbailon-at-email.phys.polymtl.ca"} jbailon-at-email.phys.polymtl.= ca {/A} ).=20 Arrangements will be made for the duplication of the documents and for = their=20 copyright. Obviously, due credits will be given in the CD-ROM for the = authors of=20 these documents. Many thanks in advance. {/FONT} {/P} {/DIV} {DIV} {SPAN class=3D300292522-26021999} {FONT color=3D#000000 face=3DArial =
I am trying to return an older microscope to service. It is a Reichert, I think of 1960's or 1970's vintage, and has been "in storage" ( ie. collecting dirt) for about 15 years, so its moving parts are frozen. It has only a serial number for identification; Nr. 236 628. It is a big black microscope with trinocular head and camera. The light source is rear-mounted, the lamp housing making a right angle with a tube containing a focuser for the bulb filament, a diaphragm, and two filter holder slides. It provides sub-stage illumination through the base, from an external transformer. There are "toggles" on either side of the base for switching to "Epi" or "Mix" illumination. The condenser looks like a dedicated phase type, Nr. 13 259. The objectives are Plan 4:1 (brightfield), Ph. 20:1, Ph. 44:1, and 100.1 (also appears to contain a phase ring, and has an internal diaphragm). Does this description ring bells for anyone? I used the same model microscope briefly back in the early 70's, but this one has a different condenser. I can't recall how to set it up properly as I have not used phase contrast since then. I seem to recall that something called a "phase telescope" (??) was used in alignment. Of course I can't find any other parts or the manual!
Could anyone offer advice on how to set it up correctly? Or know how I could get a manual? The lamp assembly is puzzling; the bulb is so large that it is touching the housing. I wonder if it is the correct bulb - any ideas?
To all who desired more info on fixing and staining of liposomes-- I apologize for the delay in my response. We had a major snowstorm and I lost a day. The initial query by Bernward Laube was about evaluating homogeneity of liposome suspensions ( {100nm diam) and estimating particle volumes. We have been using a osmium fix/negative stain technique for a number of years to look at size distribution of VLDL, chylomicrons, and synthetic lipid emulsions. The osmium fix firms up these particles nicely so that flattening is greatly reduced. In fact, based upon metal shadowing exps. the slight enlargement of diameter due to flattening after fixation is negated by slight shrinkage of particles during processing. The result is an excellent estimate of diameter and volume.
In regard to lipid vesicles/liposomes, our experience with smaller ones of 50nm or so prepared by sonication and processed with only negative stain is that they are preserved pretty well. We would expect some flattening although we have not shadowed these. However, with larger liposomes of 100nm+ diam, we believe OsO4 will aid in preservation and stability and presumably reduce flattening.
If one requires differentiation of lamellar structure (uni vs multi) among particles within a population, negative staining is unreliable because of variability of stain penetration. For this purpose, we use vitreous ice cryomicroscopy as we are fortunate to have it available.
In general our fixation/negative staining procedure is as follows:
1) Add 1 volume of 2% OsO4 in Cacodylate buffer to 2 volumes of emulsion or liposome prep. Fix for 30 mins.+
2) Place small aliquot on freshly flow-discharged carbon formvar-coated grid for a few seconds.
3) Remove and blot off excess fluid and immediately stain with 1% NaPT.
4) After a few seconds, remove and blot excess fluid. Air dry.
Optimum concentration is trial and error. Fixed suspension can be diluted as needed. Hope this helps.
Don Gantz Biophysics Dept Boston Univ Med School gantz-at-med-biophd.bu.edu
As the applications and inquiries about the UBC Live-cell Course have come in, it has become clear to me that some of the descriptions of it may have given some wrong impressions.
Two of the common misconceptions seem to be:
1. That you will not be welcome unless you have a live-cell project.
2. That you should have a very high level of confocal experience before attending.
In fact, many students participate fully in the course even though they do not come with their own live-cell project. This is because they are paired in small groups with others who do have such projects. We stress the "live-cell" emphasis in the course for two reasons:
- We think of it as the most difficult type of 3D microscopy to do. Therefore, learning to do it, will teach the best techniques in other areas.
- We think that the ability of LM techniques to perform live-cell studies is one of their greatest advantages: one that should not be thrown away when one moves to 3D.
However, many excellent images are produced each year from specimens that have been fixed and stained. (Try http://corn.eng.buffalo.edu/19983d.htm and http://confo.pulmonary.ubc.ca/~dietrich for some examples)
On the second point, it is true that many who have attended in the past have run major microscopy facilities for years, it is also true that others have had only slight prior experience.
We start with Kohler illumination (taught by Ernst Keller from Carl Zeiss) and go on from there. In addition, this year we will add a one-day pre-course meeting with Ernst for those but who really haven't thought seriously about microscopes before, although they may have used them.
The best news is that, because of a misprint in one of our announcements, the deadline for applications has been extended to March 15.
Hope to see you there,
Jim Pawley
PS: For more info: see http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
**************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
Dear Microcopists: In my recent study of Si dislocations caused by MeV ion implantation and annealing (long time), I found that many linear dislocations in the implanted area. In the attached file (observed along [110] of (001) Si), there two kinds of dislocations. One on {111} planes and running along {112} directions and the other on (001) plan and enlongated along {100} . I am sure there are not {311} dislocations either the Frank loops. But I can't give a defination of these dislocation with a Burgers vector, because my HRTEM is not suitable to do that defect analysis. Hope some one can give me some suggestions about this dislocations (how to call them and what's their possible B vector and they are partial or perfect dislocations). I realy appreciate any information about it. Thank you very much.
{ {dislocation draw.doc} } DAI Jiyan IMRE Singapore
The microscope is (almost) certainly a Reichert Zetopan. This famous stand was made in the Reichert factory in Vienna, Austria and was discontinued in1975. Reichert is now part of the Leica group. I can send you a copy of the manual (multiple page *.tif file, zipped, about 1M).
I have included a (small) picture of the Zetopan from the manual for identification purposes...
Consider yourself lucky: this is one of the best microscopes ever made!
You may want to double check and see if Ar really flows into the chamber 'cause lighter ions MAY NOT be able to knock off atoms off your target. -cy
On Wed, 24 Feb 1999, George Lawton wrote: } Thanks to all who responded to my problem. } But I still have the problem. I got a new cylinder } of Argon. I checked the hoses to make sure they } were not clogged. I double checked } the polarity. My vacuum is good. But when I } turn on my DSM-5 I get a blue arc around the } middle of the cathode. After 3 minutes, I shut } the sputter down but I have no coating on my } sample. I checked the inside of the DSM-5 and } found no loose wires, and the fuses were good. } Any other suggestions would be greatly } appreciated. } } George Lawton } Chief Electron Microscopist } Microscopy and Imaging Service Center } UT Southwestern Medical Center at Dallas } Phone: 214-648-7291 } eMail: George.Lawton-at-email.swmed.edu
In a message dated 2/27/99 12:13:30 AM, jbailon-at-phys-server.phys.polymtl.c= a writes:
} ...In Materials Science, some topics such as the glide of dislocations on } a slip plane, cross-slip of dislocations, pile-ups of dislocations } along grain boundaries, Frank-Read sources, Orowan=92s mechanism are } difficult to teach with only the help of traditional 2D figures in a } book...
I couldn't agree more. Have you seen the diagrams, animations and video cl= ips in our ViMS (Visualizations in Materials Science) CD? (http://www.pws.com/ge/russ.html, PWS Publishing Co) The CD is available f= or both Mac (ISBN 0-534-95052-3) and Windows (ISBN 0-534-95736-6) users. The = CD is in use along with or in some cases in place of a traditional textbook a= t more than 40 universities, worldwide. When our own classes are not in sess= ion at N. C. State University, you can access them via the web at http://vims.ncsu.edu
I have been trying to find the fax or email of Leitz Microscopes. I have been unable to find it after a lot of efforts on the web. Can anybody pass me the relevant info so that I can get in touch with their sales people.
{html} {head} {/head} {BODY bgcolor=3D"#FFFFFF"} {p} {font size=3D2 = color=3D"#000000" face=3D"Arial"} I have been trying to find the fax or = email of Leitz Microscopes. I have been unable to find it after a lot of = efforts on the web. Can anybody pass me the relevant info so that I can = get in touch with their sales people. {br} {br} Thanks {br} {br} Dr.Usman = Rafi {/p} {/font} {/body} {/html} ------=_NextPart_000_01BE628A.433AF9E0--
In a message dated 99-02-27 10:13:27 EST, pulse-at-shoa.net writes:
{ { I have been trying to find the fax or email of Leitz Microscopes. I have been unable to find it after a lot of efforts on the web. Can anybody pass me the relevant info so that I can get in touch with their sales people. } }
Dr. Rafi,
Leitz is now part of the Leica group (along with Reichert-Jung, American Optical, Bausch & Lomb and Cambridge Instruments). The address for Leica will depend on what country you are in, but the easiest way to contact them is at their website:
www.leica.com
} From there you can fill out a request form, and it will be passed to the group which represents Leica in your area.
Best regards,
Bob ****************************** Robert (Bob) Chiovetti, Ph.D. President Microimaging Technologies, Inc. Tucson, Arizona USA Tel./Fax (520) 546-4986 rchiovetti-at-aol.com Manufacturers' Representatives Systems Integrators Analog & Digital Imaging Systems Clinical & Research Microscopy Cytology/Histology/Pathology/EM *******************************
It sounds as though you have a very treasured microscope called a Zetopan. If you can take a picture (even a polaroid) and send it to me, I can confirm that.
If, indeed, this is the instrument you have, I can supply supportive documentation (manual, brochure with decription of parts, etc.). Since this microscope was changed to a dramatically different design in the early 60's (the Polyvar), I suspect that it may be older than you think.
Setting up phase is simple: (I know that this looks like a large number of steps, but once you get the hang of it, it should take less than a minute) 1. Put a well-behaved sample (thin section or cheek smear) on the stage and bring it into focus using the 20x Phase Objective. Set the condenser to brightfield (either BF or H). 2. Close the field iris (on the light port) until you can see it in the field of view. Set up regular Koehler illumination. (If you need a review: a. Focus the image of the field iris using the focus control on the condenser mount b. Center the image of the field iris using the centration screws at about 5 o'clock and 7 o'clock) When you are done, open the field iris so that it is just outside the field of view. 3. Replace one of the eyepieces with the "phase telescope". If you can't find the phase telescope, just remove one of the eyepieces and look way down the tube (this plane is called the Back Focal Plane of the Objective and what goes on there is critical to good imaging). You will see a smokey ring (the phase plate). 4. Rotate the condenser so that the "PH20" aligns with the white marker on the condenser. Look back down the tube/phase telescope. You should see a bright ring. Align the bright ring so that it sits completely and exactly under the smokey ring. I don't have either my manuals or my microscope here at the office, so I am not sure where these alignment controls are. Frequently they are sliders on the rotating plate and/or secondary centering screws on the condenser rather than on its mount. TROUBLE-SHOOTING: If the ring is not the same size, rotate through all the options until you find the best match. If it is not exactly in focus as you look down the Back Focal Plane, use the condenser focus to rack the condenser up or down slightly. If you cannot see the ring, check to make sure that both the field iris and, especially, the condenser aperture iris are open. Most condensers are designed so that this second iris is out of the way when you rotate to the Phase position, but not all. Finally, Phase works best if you insert a good quality green (546nm) filter over the light port. However, the illuminator for the Zetopan may not be powerful enough to supply adequate illumination. In this case you have two options: (a) work without the green filter or (b) contact me privately. I am having my Zetopan illuminator "modernized" shortly and may have some alternatives for you.
For further information on how Phase Contrast works and how to optimize it, see our book "Optimizing Light Microscopy". Details are on our website: MME-Microscopy.com/education
Hope this was helpful.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 06:07 PM 2/26/99 -0500, Sharon Godkin wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Finally AFM that compliments the SEM lab.=20 =20 AFM designed for imaging in fluids and under controlled environmental = condition. =20 Capable of force spectroscopy measurements down to the piconeuton of = force (protein folding or antibody - antigen binding force = characterization) with the same instrument that can measure very soft = biological samples at 37C with better that 1 manometer resolution.
Some images http://www.molec.com/biology/index.html
Hands on workshop (bring you own samples) Detail will be posted on = www.molec.com in about a week. In Situ Surface Chemistry 4/27 to 4/28 In Vitro Biology 4/28 to 4/30
If two peaks are overlapped, and you want to distinguish between the two, you can increase the acceleration voltage, right? But how does this help to distinguish between the elements?
My next question concerns Energy Dispersive X-ray Spectroscopy (EDS). The baseline on the EDS spectra is influenced by inelasic scattering of the incident electron beam by the atomic nuclei of the sample, which results in a peaked background. This is called the bremsstrahlung effect. What causes this???
I don't get your first question. You are probably referring to the EDS peak overlap. If so, I don't think increased acceleration voltage can increase the EDS resolution directly. Instead, higher voltage may generate/promote peaks with higher characteristic energy values. These peaks may not overlap and therefore can be distinguished.
A simple answer to your second question is that X-ray photons are not only, though mainly from the sample, but also from everywhere in the chamber because of the elastic/inelastic scattering of electrons.
Hope the above helps.
-cy
On Sat, 27 Feb 1999 Seadoohog-at-aol.com-at-sparc5.microscopy.com wrote:
} I just have a couple of questions regarding SEM. } } If two peaks are overlapped, and you want to distinguish between the two, you } can increase the acceleration voltage, right? But how does this help to } distinguish between the elements? } } My next question concerns Energy Dispersive X-ray Spectroscopy (EDS). The } baseline on the EDS spectra is influenced by inelasic scattering of the } incident electron beam by the atomic nuclei of the sample, which results in a } peaked background. This is called the bremsstrahlung effect. What causes } this??? } } Thanks! } } } }
Does any one have any experience with the $279.00 eyepiece camera that www.imicrovision.com sells? It is a CMOS camera that is read through the printer port.
It claims a 704 X 576 max resolution.=20
I know that CMOS cameras are noisy. But it looks like it might be an adequate illustration tool. If I want resolution I can use the 4X5 camera and do 3 color separations.
Thanks Gordon
Gordon Couger gcouger-at-couger.com Owner PRAG-L PRactical AGriculture List www.couger.com/prag-l Stillwater, OK 405 624-2855 GMT -6:00
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD}
{META content=3Dtext/html;charset=3Diso-8859-1 = http-equiv=3DContent-Type} {!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 = HTML//EN"} {!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {FONT color=3D#000000 size=3D4} Does any one have any experience = with the=20 $279.00 eyepiece {/FONT} {/DIV} {DIV} {FONT color=3D#000000 size=3D4} camera that {A=20 href=3D"http://www.imicrovision.com"} www.imicrovision.com {/A} sells? It = is a CMOS=20 camera {/FONT} {/DIV} {DIV} {FONT color=3D#000000 size=3D4} that is read through the printer=20 port. {/FONT} {/DIV} {DIV} {FONT color=3D#000000 size=3D4} {/FONT} {/DIV} {DIV} {FONT size=3D4} It claims a 704 X 576 max resolution. {/FONT} {/DIV} {DIV} {FONT size=3D4} {/FONT} {/DIV} {DIV} {FONT size=3D4} I know that CMOS cameras are noisy. But it looks = like it=20 might {/FONT} {/DIV} {DIV} {FONT size=3D4} be an adequate illustration tool. If I want = resolution I can=20 use the {/FONT} {/DIV} {DIV} {FONT size=3D4} 4X5 camera and do 3 color separations. {/FONT} {/DIV} {DIV} {FONT size=3D4} {/FONT} {/DIV} {DIV} {FONT size=3D4} Thanks {/FONT} {/DIV} {DIV} {FONT size=3D4} Gordon {/FONT} {/DIV} {DIV} {FONT size=3D4} {/FONT} {/DIV} {DIV} {FONT size=3D4} Gordon Couger {A=20 href=3D"mailto:gcouger-at-couger.com"} gcouger-at-couger.com {/A} {BR} Owner = PRAG-L=20 PRactical AGriculture List {A=20 href=3D"http://www.couger.com/prag-l"} www.couger.com/prag-l {/A} {BR} Stillw= ater,=20 OK 405 624-2855 = GMT=20 -6:00 {/FONT} {/DIV} {/BODY} {/HTML}
---------- } Van: Barbara Foster {mme-at-map.com} } Aan: Sharon Godkin {GodkinS-at-em.agr.ca} ; microscopy-at-sparc5.microscopy.com } Onderwerp: Re: LM - old Reichert; need manual } Datum: zaterdag 27 februari 1999 19:02
Hi all,
According to Mr. Kappl, head of the service department of the Reichert-factory in Vienna, Austria (now Leica-Reichert), the Zetopan was discontinued in 1975 (personal communication, 1995).
There were two models of the Zetopan: the (older) Zetopan with black finishing, and the (more recent) one with grey finishing. Essentialy these two are the same except that some spare parts have other dimensions (i.e. the UV-block filterholder).
Some spare parts (new/second hand) are still availlable trough some German and Austrian dealers (bought some time ago a new micro flash unit for Zetopan and some filter holders).
Second-hand Zetopans aren't to expensive (if you can find one). Judging from the prices on some online auctions, Zetopan is far less expensive than comparable instruments made by Zeiss and Leitz (Leica). This puzzles me as the Zetopan is IMHO far better than those, spare parts are (more or less: with luck and time) availlable at decent prices (at least in Europe).
One problem is, that the bulbs used in the illuminator "LUX FNI" are difficult to find. It's probably interesting to know that most European microscopes are using either Osram-bulbs or some kind of modified Osram-bulb. This is also the case for the lightsource "LUX FNI". The bulb used is essentialy an Osram nr. 8100 with E14 foot (BTW: this is the same bulb as the one used in another famous microscope: the Leitz Ortholux...) The bulb is soldered in some kind of a "Reichert adapter", thus providing a "precentered and preadjusted" bulb... It's perfectly possible to remove the old bulb, clean up this adapter and fit a regular Osram 8100 in it. That bulb is also far less expensive than the "Reichert bulb"...
At least its true that electrons scatter like light and X-rays with the characteristics of the last reflecting surface (sufficient oomph permitting) are generated. However, EDS detectors have a collimator tube which limits most of the scattered X-rays. In any case the percentage of scattered X-rays is small - to wit: this type of analyses is very location specific and on polished specimen can give very accurate results.
Bremsstrahlung (literally "Braking Radiation") is also called continuum. Peaks sit on top of this background radiation. Lower kV results in a higher continuum as does an average low atomic number specimen. Continuum is the product of electron interaction with the nucleus and not other electrons.
Remember that most EDS/WDS analysis is done at 15kV. KV is varied to identify problem peaks. Perhaps to see another higher peaks or obtain better resolution in the low end of the spectrum. Change from 15kV is there is a good reason only. Disclaimer: ProSciTech has no bremsstrahlung but plenty of continuum on offer. Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Sunday, February 28, 1999 12:05 PM, Chao-ying Ni; Office 312 SPL; Phone 1013 [SMTP:ni-at-me.udel.edu] wrote: } } } I don't get your first question. You are probably } referring to the EDS } peak overlap. If so, I don't think increased acceleration } voltage can } increase the EDS resolution directly. Instead, higher } voltage may } generate/promote peaks with higher characteristic energy } values. These } peaks may not overlap and therefore can be distinguished. } } A simple answer to your second question is that X-ray } photons are not } only, though mainly from the sample, but also from } everywhere in the } chamber because of the elastic/inelastic scattering of } electrons. } } Hope the above helps. } } -cy } } } } On Sat, 27 Feb 1999 } Seadoohog-at-aol.com-at-sparc5.microscopy.com wrote: } } } I just have a couple of questions regarding SEM. } } } } If two peaks are overlapped, and you want to distinguish } } between the two, you } } can increase the acceleration voltage, right? But how } } does this help to } } distinguish between the elements? } } } } My next question concerns Energy Dispersive X-ray } } Spectroscopy (EDS). The } } baseline on the EDS spectra is influenced by inelasic } } scattering of the } } incident electron beam by the atomic nuclei of the } } sample, which results in a } } peaked background. This is called the bremsstrahlung } } effect. What causes } } this??? } } } } Thanks! } } } } } } } } }
This is a multi-part message in MIME format. --------------FFDDB42C033F1E52239010A3 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit
We had a similar problem. Eventually the horizontal banding became so bad we thought we needed a new detector or at least a new photomultiplier tube in the detector. The banding was absent when we switched to our Robinson detector. It turned out to be a loose grounding wire in the detector unit itself. See if wiggling the wires coming out of your detector effect your image (e.g. increased banding, lost of contrast, bright white screen), if it does, then the solution is simple.
Roy Nelson Material Testing Laboratory Pennington, NJ 08534 jrnelson-at-nj1.aae.com
P.S. It took forever for your images to load. --------------FFDDB42C033F1E52239010A3 Content-Type: text/x-vcard; charset=us-ascii; name="jrnelson.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for jrnelson Content-Disposition: attachment; filename="jrnelson.vcf"
Does the banding appear in your secondary images? If so there may be a contaminant in the column or on the BSED itself. Slide the BSED out and see if the SED image improves. (I believe Robinson detectors can be moved in or out). If the banding in your SED imaging goes away with the BSED out, there is probably a piece of "dirt" on the forks of the BSED. Try to clean the forks with a blast of nitrogen or other aerosol. Careful if you have to clean the forks of the BSED by wiping them, there is a thin conductive coating on the Lucite forks which comes off easily. Also check the metal strip on top of the BSED forks with an ohmeter to make sure it makes good electrical contact with the detector tube. (As long as you have an ohmeter out, make sure your sample is indeed grounded by measuring the resistance from your coated sample to ground.) Also inspect the bottom of the objective lens for any contaminants. If the banding happens in both SE and BSE modes, any contaminant in the column, or even an ungrounded aperture in the beam path is suspect. Also, make sure the beam emission current is stable. Hope this helps!
Bill Carmichael
"D.Wild" wrote:
} Dear all, } We are imaging pumice sections on glass slides, using BSE detector at 25kv, } condenser lens 4, using a Robinson BSE detector on a Hitachi FE4000 The } idea is to obtain highly contrasted images to transform into black and } white binary images. The problem is we are getting horizontal banding which } looks like a charging problem, although the samples are well coated and } earthed. Could it be due to anything else? The problem has recently } developed and was also apparent on a more conducting sample of some TEM } grids. Attached are some images. } Any comments would be helpful. } } Thank { {horiz_bd.tif} } { {18299_29.tif} } s David Wild } } ------------------------------------------------------------------------ } Name: horiz_bd.tif } horiz_bd.tif Type: TIFF Image (image/tiff) } Encoding: base64 } } Name: 18299_29.tif } 18299_29.tif Type: TIFF Image (image/tiff) } Encoding: base64
Actually the bremsstrahlung, (I think is German for "braking radiation") is from the beam/specimen interaction, and infact, can tell you much about the specimen surface. As I understand it, the bremsstrahlung is the result of the inelastic scattering events that occur when the high energy beam electrons interact with the specimen. As the primary electron (beam) approaches the specimen, it is affected by the electrons and nuclei of the specimen atoms, and it loses energy. The energy lost is released in the form of a photon with a given energy. These events are quite random and the energies of the photons are also quite random, with photon energies ranging from as high as the beam energy, down to small fractions of the beam energy. These photons when detected by the EDS system give a continuous background of energy counts from nearly zero to the accelerating voltage energy. By the way this is a good way for determining the actual high energy (accelerating voltage) that is being emitted by your SEMs "electron gun".
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I just have a couple of questions regarding SEM.
If two peaks are overlapped, and you want to distinguish between the two, you can increase the acceleration voltage, right? But how does this help to distinguish between the elements?
My next question concerns Energy Dispersive X-ray Spectroscopy (EDS). The baseline on the EDS spectra is influenced by inelasic scattering of the incident electron beam by the atomic nuclei of the sample, which results in a peaked background. This is called the bremsstrahlung effect. What causes this???
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Dear All
My vote would be that ads for the disposal of used equipment by the owner are OK but that ads by dealers are not. I hope that this is the rule.
Ritchie
} I was under the impression that this server was NOT the place for posting } items for sale. } If that is the case then ignore this message. } If I have been wrong or the rules have changed then fellow readers I have } some equipment that is available: } } Sorvall MT-2 ultramicrotomes(2). } Mettler analytical balance H78AR } TMC Micro-G pneumatic table 24x40 } AFM probes } GAST compressor/vacuum pump 60psi. } } All are in excellent working order and are being offered at substancially } reduced prices. } I need the cash. } } Contact; } Steve D'Angelo, Pacifica California, 650.738.2699, 650.400.8063 or email } sdangelo-at-batnet.com
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Hi there listers, Someone in our lab is about to start a project on microvascular casting of varicose veins for SEM viewing. We were wondering what most peoples preference was for some of the commercial polymers and kits available? Your ideas of pro's and con's for these products would be nice too!
Look forward to hearing from you,
Rich.
----------------------------------------------------------------------- Richard Lander Electron Microscopist South Campus Electron Microscope Unit Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand. Tel. National 03 479 7301 Fax. National 03 479 7254 mailto:richard.lander-at-stonebow.otago.ac.nz "Southernmost EM Unit in the World!" ------------------------------------------------------------------------
-----Original Message----- } From: "Seadoohog-at-aol.com"-at-sparc5.microscopy.com {"Seadoohog-at-aol.com"-at-sparc5.microscopy.com} To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}
I am wanting to label some ultra thin sections of marrow embedded in LR White for alkaline phosphatase for viewing on the TEM. Can any-one help me with a method, I have heard that the use of lead citrate is the way to go. My specimens were originally fixed in either 4% paraformaldehyde, or 2% paraformaldehyde and 0.05% glutaraldehyde, then decalcified in 10% EDTA. Do I need to reactivate the enzyme with Mg2+ treatment? If so how? Thanks, Susie Nilsson PhD. Peter MacCallum Cancer Institute Melbourne, Australia s.nilsson-at-pmci.unimelb.edu.au
I appreciate FS posts. For those that do not, it would be easy for the poster to preface their post in the Subject block with FS: xxxxx that way, those that do not want to see FS items can filter them out.