Birna wrote: My name is Birna Gu=F0bj=F6rnsd=F3ttir working at Icelandic Fisheries Laboratories as a food scientist. This field is quite new to me and I am looking for information how I can prepare wood samples, both wet nad dry, for SEM. Any information would be greatly appreciated.
Dear Birna,
for conventional SEM your samples have to be dry. If your sample is already dry, just sputter with gold. For wet wood it is depending on the age of the the wood and the quality you want. Maybe air drying is possible? If not you have to perform critical point drying or freeze drying.
Best wishes from Anne Heller
Dr. Anne Heller Arbeitsgruppe Elektronenmikroskopie Institut fuer Botanik (210) Universitaet Hohenheim Garbenstr.30 D-70593 Stuttgart Tel.0049-711-459-2180 Fax 0049-711-459-3355
hi everybody ! What is specific to surface profile imaging technique in HRTEM ? Is it classical HRTEM used on surface imaging and profile only means that HRTEM images are used to deduce the structure (=profile) of the surface (=edge) of the microcrystal in a classical way (structural models + simulations) or is there a specific technique ? Thanks B. DOMENGES CRISMAT - ISMRA Boulevard du Marechal Juin 14050 CAEN CEDEX France E-mail : domenges-at-crismat.ismra.fr Tel. : (33) 02 31 45 26 32 Fax. : (33) 02 31 95 16 00
Sandra: Pardon a copied reply, but this is a common problem with phosphate buffer fixation. I posted the following reply to a similar request in September '98.
Sally, about 15 years ago somebody published the reason for this Os "pepper". Simply, for this to occur, some chemically unbound Os, GA and phosphate must remain in the tissue. If any one of these is missing you will not get this frustrating artefact.
I suppose this is one major reason for the popularity of cacodylate buffer. It makes the very thorough rinsing process which is required between GA and OS redundant; simply any unbound GA no longer matters.
Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Thursday, April 01, 1999 5:10 AM, Sandra Perkins [SMTP:skperkin-at-vt.edu] wrote: } ---------------------------------------------------------- } -------------- } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht } ml } ---------------------------------------------------------- } -------------. } } } Hi- } } First, thanks to everyone who responded to my question on } fixation of cells } grown on chambered Permanox slides. As soon as I try the } suggested } procedures (the cells crashed!), I will post a summary of } responses to the } list. } } My question today has to do with the presence of "pepper" } on TEM sections. } We process rather large tissue blocks (4X5 mm) of nervous } tissue that we } have dissected from perfused animals (5% glut. in PB is } routine perfusate). } Post-fixation is in osmium tetroxide in PB and all buffer } rinses are done } in PB. We dehydrate using an ethanol series, transition } through propylene } oxide (although we are experimenting with ethanol with no } succes so far) } and infiltrate/embed in PolyBed 812. The pepper shows up } most obviously } on the cytoskeletal elements of nerves, mitochondria, and } collagen. I have } tried pretreating the sections with 0.5-1.0 N HCl or 1% } EDTA (Mollenhauer, } 1987)prior to staining with uranyl acetate/lead } citrate.....no luck. I } can visualize the pepper on unstained sections. I seem to } recall that this } may have something to do with phosphate buffer, but the } details are hazy. } I would appreciate any suggestions. } } Thank you very much! } } Sandy Perkins } }
Hi Terry: I found scoring wheels available at a local glass supplier. You may have to purchase a glass cutter and then remove the wheel. The wheels I bought had a smaller diameter, but they worked well for many years. The wheel diameter does not affect any calibration since the clamping position and pressure are determined by they weight of the head.
If needed, the axle could be replaced by part way grinding and then breaking a piece from the shaft of a small drill. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Thursday, April 01, 1999 9:51 AM, Terry Robertson [SMTP:terryr-at-cyllene.uwa.edu.au] wrote:
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht } ml } } Does anyone involved in Electron Microscopy know where I } can obtain spare glass scoring wheels for an LKB 2178 } Knife Maker II. Any helpful advise would be welcome as } our machine requires a replacement scorer ASAP. } } Dr Terry Robertson (PhD) } Senior Research Fellow } Department of Pathology } University of Western Australia } Nedlands 6907 } } Phone 618 9346 2935 } Fax 618 9346 2891 } Mobile phone 618 0415 986531 } email terryr-at-cyllene.uwa.edu.au } }
Sandra, Regarding your problem with pepper on sections, I have seen that phenomenon in sections that had not been buffer washed enough after glut. Your blocks are very large and so may require longer and more requent washing.If the glutaraldehyde is not completely washed out it will form a ppt with osmium. You might also check the ph of your buffer. It should be around 7.4 for mammalian tissue.
Mary Gail Engle Electron Microscopy & Imaging Facility University of Kentucky
Subject: Polymers SPM -----Original Message-----From: george sibbald {geos-at-goldrush.com} To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com} , Barbara Foster {mme-at-map.com} Date: Tuesday, March 30, 1999 6:45 PMSubject: Polymers SPM Barbara Although Molecular Imaging was not at Pittcon, MI also has products for polymer several with significant technical advantage. eg: Temperature, environmental control, MAC Mode, "Pulse Force" and Force spectroscopy. George Sibbald -----Original Message-----From: Barbara Foster {mme-at-map.com} To: Janet H. Woodward {jhwoodward-at-buckman.com} ; microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com} Date: Monday, March 29, 1999 5:37 PMSubject: Re: SPM of Polymers------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.Dear Janet,SPM of Polymers is one of the hottest new trends in microscopy. I am in the middle of writing a review of "What's New in Microscopy at PITTCON" (May, 1999, American Lab) and this topic was well represented by new offerings from several companies. I'd suggest you visit the websites for 1. Digital Instruments - they have lots of great app notes on this topic2. ThermoMicroscopes (previously known as Park Scientific and Topometrix) and look for information on the new Explorer PolymerSystem SPM (integrated microthermal analysis and pulsed force mode imaging)3. JEOL - their new SPM has a full hot/cold stage which runs from 130K to 800K, for watching thermal transitions.Other relevant technology I found at Pittcon included the new thermal stage in Philips' ESEM, the new Continuum microscope for full microscopy imaging (Including DIC) integrated with FTIR from SpectraTech, and a number of interesting systems for Raman/confocal from Renishaw and Instruments SA.Hope this "preview" was helpful.CAVEAT: Neither Barbara Foster nor MME has any commercial interest in any of these instruments.Best regards,Barbara FosterConsortium PresidentMicroscopy/Microscopy Education ...Educating microscopists for greater productivity.125 Paridon Street Suite 102 Springfield, MA 01118PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.comVisit our web site {http://www.MME-Microscopy.com/education} *************************************** ***************MME is America's first national consortium providingcustomized on-site workshops in all areas ofmicroscopy, sample preparation, and image analysis.At 10:29 AM 3/29/99 -0600, Janet H. Woodward wrote: } } } } My company's R&D group is interested in visualizing several of our polymers. The M&M '99 session, "Developments in Scanned Probe Microscopy of Polymers", will be helpful. However, we would like some basic/general information prior to the meeting. Any suggestions of review articles, textbooks, etc. would be greatly appreciated.In advance, thanks!Janet H. Woodward, Ph.D.Corporate Technical Specialist - Microscopy & MicrobiologyBuckman Laboratories1256 N. McLean StreetMemphis, TN 38108(901) 272-6408 {mailto:jhwoodward-at-bbuckman.com} jhwoodward-at-bbuckman.com { { { {
Several years ago I bought some at the local hardware store that fit our LKB 7801A, they were the standard replacements for commercial window glass cutters. If I remember rightly they were only about a dollar each.
Terry Robertson wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Does anyone involved in Electron Microscopy know where I can obtain spare glass scoring wheels for an LKB 2178 Knife Maker II. Any helpful advise would be welcome as our machine requires a replacement scorer ASAP. } } Dr Terry Robertson (PhD) } Senior Research Fellow } Department of Pathology } University of Western Australia } Nedlands 6907 } } Phone 618 9346 2935 } Fax 618 9346 2891 } Mobile phone 618 0415 986531 } email terryr-at-cyllene.uwa.edu.au
--
Richard J. Mount Auditory Science Laboratory, Department of Otolaryngology & Brain and Behaviour Division/Research Institute The Hospital for Sick Children Toronto, Ontario, Canada (416) 813-6551; Fax (416) 813-8456 http://www.sickkids.on.ca/HSCWeb/Otolaryngology/Otoalias/Earhome1.htm
I am looking for a software package or algorithm to produce 3D images from stereo-pair images (LM or SEM). From the 3D images, we want to extract information such as distance, height, angles, volumes, etc. Any suggestions would be warmly welcome!!
My Safety Officer wants all our mechanical pumps vented outdoors. The pumps are Sargent-Welch, Alcatel, and Edwards and are used to back TEMs, film desiccators, evaporators, etc. Initially I thought this was a good idea, having done it simply in past lives by drilling a hole in a wall, ganging the outlet hoses, and presto - out the bad smoke went.
However the B&G engineering consultant got hold of the project (yes now it's a 'project') and now EVERY pump must get its own line up to the roof. This means each pump's discharge is directed to a run of 1-inch copper pipe with three to five 90-degree bends over a total vertical length of about 20'. (Luckily I am on the top floor.) There is also talk about inserting a clean-out or filter at each feed-through for dealing with accumulated oil.
I'm concerned that the pumps are not designed for backpressure on the outlet side coming from the 20' column of air plus the resistence from the 90-degree bends and filter.
Could this setup affect the (1) efficiency or (2) overall life of the pumps? Am I being overly cautious?
I'd appreciate feedback from the List (including manufacturers) re the feasiblity of this approach, and the pump specs - I haven't been able to find anything about discharge 'load' tolerances.
Thanks, you can reply offline and if there is sufficient interest I'll summarize responses for the List.
Ann Hein Lehman EM Facility Mgr Trinity College Hartford, CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-exchange.cc.trincoll.edu
Postdoctoral Position to Study the Microstructure and Chemistry of Catalyst Materials
The Materials Analysis User Center (MAUC) at the Oak Ridge National Laboratory in Oak Ridge, Tennessee, is seeking a postdoctral candidate to perform research using primarily electron microscopy and surface chemistry to characterize the structure and chemistry of catalysts and other materials. This position will support Department of Energy research programs aimed at reducing both gaseous and particulate emissions from gasoline and diesel engines. The researcher will work with others who are producing and performance testing advanced catalyst formulations. The researcher will be expected to use high resolution electron microscopy and other tools to relate structure at the atomic level to performance. Although a small portion of the research will be proprietary, most will not, and the researcher will be expected to present and publish results. The successful candidate must have a PhD (in materials science, surface chemistry, or a related discipline), experience characterizing catalyst materials using TEM and/or STEM; and should have a strong computer background (the MAUC is entirely digital). The researcher should also have experience with other analytical tools such as FEG-SEM, FEG-SAM, XPS, or electron microprobe, and experience characterizing a wide range of other non-catalyst materials. The researcher will work with many DOE contractor, industrial and university researchers, including some as a part of DOE-ORNL user programs; thus he or she should enjoy collaborating with others. This position is for one year, extendable to two.
ORNL, a multipurpose research laboratory managed by Lockheed Martin Energy Research Corporation for the U.S. Department of Energy, is an equal opportunity employer committed to building and maintaining a diverse work force.
Please send curriculum vitae and bibliography to:
Ted Nolan Manager, Materials Analysis User Center High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 Oak Ridge, TN 37831-6064
Dear Mr. Li, Although I don't have experience etching gold myself, the chemical recommended in the Vander Voort book for gold is aqua regia, a mixture of HNO3 and HCl. Either a 60% HCl, 40% HNO3 or 60 ml. HCl, 20 ml. HNO3, used boiling, is recommended. Always use aqua regia fresh, in a fume jood and never cap it, as it evolves gas. We had a nasty explosion here from sommeone who screwed the cap on a two liter bottle of aqua regia. The MSDS os the separate acids do not prepare one for this hazard. You wrote:
} Hi, } } We are trying to study the microstructure of cold drawn 25 =B5m gold= wires. } We meet the difficulty in etching the gold wires to reveal the grain } structure in SEM and optical observations. Could somebody give us some } suggestions on how to etch the wires. } } Thanks in advance! } } Best regards, } } Kun Li } } Kun Li, Ph. D } Institute of Materials Research and Engineering Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
} I am looking for a software package or algorithm to produce 3D images from } stereo-pair images (LM or SEM). From the 3D images, we want to extract } information such as distance, height, angles, volumes, etc. Any suggestions } would be warmly welcome!! } } James Ma } 3M Building 201-1E-15 } St. Paul, MN 55144 } Some years ago we were interested in doing similar things. You would expect the algorithms to have been all worked out for problems like aerial photography, remote mapping of Mars and Venus, etc. etc., and indeed, for those applications they have been. I worked, with colleagues, with Meemong Lee, then at JPL, to try to apply her programs to our images, but we could not make them work. More recently I discussed this with a friend, Tom Parr, who is a specialist in satellite imagery at The Analytical Services Corporation (TASC) in Reading, Mass. He suggested that the problem arises in extensive assumptions made by the software about the characteristics of the topology under investigation. Planetary surfaces tend to be relatively smooth, and the software has been optimise to take advantage of this characteristic. His thought was that to apply the algorithms to SEM images (in our case, of fracture surfaces in polymers) would require a re-working of the code (by someone who knew what they were doing, of course!!). Needless to say, we didn't try this!
If there is anything avaliable that would help in this problem, we, too, would still be most interested in hearing.
It has been pointed out to me that I phrased my comments regarding the header a bit harshly.
I'm sorry.
Ritchie
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
Does anyone have some advice for a novice DIC user. I have an older Leitz Diaplan that is supposed to have DIC, but it doesn't look as good as I think it should.
Can anyone suggest a good test subject for adjusting the DIC and does anyone have some good practical advice/experience in adjusting the DIC system.
Thanks
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
I have been trying all week to get in touch with the VCR company. I have three different voice mail messages with three different people there, including Mr. Carlino. Does anybody have any ideal where or what they are doing? I have one of their dimplers and I am trying rather desperately to order the 3i dimpling tool. Any help out there? Anybody by any chance have an extra one of them? I could either purchase it directly from you or when I finally am able to order one from VCR, I can replace it. Any help will be appreciated.
Thank you.
Margaret E. Bisher
NEC Research Institute 4 Independence Way Princeton, NJ 08540. Tel.: (609) 951-2629 Fax: (609) 951-2496 e-mail: peggy-at-research.nj.nec.com
Good Afternoon, Just recently, I was asked to manage an imaging core facility which actually needs to be designed and pulled together to include all imaging equipment now in the department with visions of future acquisitions. At this point in time, the major challenge is getting service for a Zeiss TEM 10C, vintage 1986 which has an X-Ray analyzer attached and a JEOL 35C SEM. Neither of these instruments is under contract. There is also a Phillips 201C, now housed in a different building, which is under contract and has been since its purchase in 1974. It is in mint condition, but because it is no longer very productive, the powers that be want to drop the contract. My question to you is, do any of you have independent service engineers servicing your scopes whom you would be willing to recommend? Or do you know of any such engineers whom I might contact? I will be most grateful for any information you might be able to provide.
Sincerely, Sandra Zane Sandra F. Zane, EM Tech. sfzane-at-email.uncc.edu Dept. of Biology, UNC-Charlotte Ph.(704)547-4051 9201 University City Blvd. Fax (704)547-3128 Charlotte, NC 28223
Our facility is going to be replacing an aging Ilford print processor soon. I have been looking at different company's products and am interested in hearing from anyone on the list who would have experience with Colex B&W print processors. In particular, I am looking at the Colette Pro model. I am not familar with this company. I am also considering the Ilford 2650.
If anyone has experience, good or bad, I would like to hear from you.
Thank you,
Jean Ross Central Microscopy Research Facility University of Iowa 85 EMRB Iowa City, IA 42242 319-335-8142 Fax 319-335-6710
We produce an image processing system called "analySIS", and one of the available modules (called "Stereo") provides the measurements you mention in your email. It also does anaglyphic images, surface rendering (heightmapped, textures, illumination), has a very fast computation algorithm, and can perform the calculations with sub-pixel accuracy.
If you need more information, please visit our website at:
http://www.soft-imaging.com
or contact me through email.
Thanks.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com
******************************************************* Disclaimer: Soft Imaging System produces and sells image acquisition and processing systems. We therefore have a vested interest in some of the items mentioned above. *******************************************************
} ---------- } From: } "jma2-at-mmm.com"-at-sparc5.microscopy.com[SMTP:"jma2-at-mmm.com"-at-sparc5.micros } copy.com] } Sent: Thursday, April 01, 1999 8:15 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Stereoscopy software } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } } } I am looking for a software package or algorithm to produce 3D images } from } stereo-pair images (LM or SEM). From the 3D images, we want to extract } information such as distance, height, angles, volumes, etc. Any } suggestions } would be warmly welcome!! } } James Ma } 3M Building 201-1E-15 } St. Paul, MN 55144 } } }
Hello, My first response would be to query whether the safety office really knows what he desires to vent with this very expensive venting project. Usually the most noxious part of a roughing pump exhaust is the particulate or oil mist. If you are not pumping something dangerous like a poison gas or something equally dreadful, I see no reason to go to the expense. The exhaust filters which have been designed for use on your pumps usually do a fine job at trapping any problem vapors. There should be no problem with back pressure. If someone finds me in error, I'm sure we will hear about it.
Good luck.
Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. Number 499, Post Office Box 19400 Austin, Texas 78760 Phone: 512/282-5507 FAX 512/280-0702
An alternate source for your electron microscope maintenance needs.-----Original Message----- } From: Lehman, Ann {Ann.Lehman-at-exchange.cc.trincoll.edu} To: Microscopy-at-Sparc5.Microscopy.Com {Microscopy-at-Sparc5.Microscopy.Com}
I would say you have nothing to fear. Typically, a roughing pump should be fitted with an oil mist filter. The back pressure generated by the filter would "swamp" any from the piping you described. My answer is predicated on pumping down a reasonably sized, sealed vacuum chamber with a rotorary vane pump or similar. After the initial few gulps of gas, the volume of gas moved (at STP) is very tiny. If your vacuum chamber is the size of a small room and the initial pumpdown is by something like a roots blower, that is different... {g}
Dear Ann, I can sympathize with your problems. I run a plastic sump pump hose from each of my rotary pumps out to the window. If you have ever watched an untrapped rotary pump, you will notice that there is almost no air movement out of the pump after the initial ten seconds of pumping. There should be no problem with long runs, bends, etc. if there is nothing but simple atmospheric pressure to oppose the air movement. A simple drain at the first bend up should drain any condensed oil, or a wad of steel wool in the line. Use as large a diameter pipe as you can get them to run. One solution I've seen is to put a commercial car oil filter onto the pump exhaust. If this didn't bother it, neither should your copper line. You wrote:
} Dear Listers, } } My Safety Officer wants all our mechanical pumps vented outdoors. The pumps } are Sargent-Welch, Alcatel, and Edwards and are used to back TEMs, film } desiccators, evaporators, etc. Initially I thought this was a good idea, } having done it simply in past lives by drilling a hole in a wall, ganging } the outlet hoses, and presto - out the bad smoke went. } } However the B&G engineering consultant got hold of the project (yes now it's } a 'project') and now EVERY pump must get its own line up to the roof. This } means each pump's discharge is directed to a run of 1-inch copper pipe with } three to five 90-degree bends over a total vertical length of about 20'. } (Luckily I am on the top floor.) There is also talk about inserting a } clean-out or filter at each feed-through for dealing with accumulated oil. } } I'm concerned that the pumps are not designed for backpressure on the outlet } side coming from the 20' column of air plus the resistence from the } 90-degree bends and filter. } } Could this setup affect the (1) efficiency or (2) overall life of the pumps? } Am I being overly cautious? } } I'd appreciate feedback from the List (including manufacturers) re the } feasiblity of this approach, and the pump specs - I haven't been able to } find anything about discharge 'load' tolerances. } } Thanks, you can reply offline and if there is sufficient interest I'll } summarize responses for the List. } } Ann Hein Lehman } EM Facility Mgr } Trinity College } Hartford, CT 06106
Best of luck! Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
To: Janet Woodward Quesant also did not exhibit its range of AFMs at Pittcon, but would like you to be aware of the fact that polymer imaging can also be done with ambient AFMs, such as ours, costing much less than the more specialized systems. Much depends on the specifics of your application. Good luck, Thomas Pelnar Quesant Instrument Corporation
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Our Coolwell "SE-Style" Chiller hooked to our TEM is on the fritz. An answering machine at the Coolwell phone number says they went out of business and refers one to Litron (or Lintron?). A call to them reveals that all they bought from Coolwell was their name and "marketing strategy". Apparently the marketing strategy is to not produce replacement parts for Coolwell chillers. So we are on our own. Does anyone have a wiring diagram, schematics, specs (such as type and amount of coolant), and/or advice for a SE Style Chiller they could share with me? Our campus refrigeration guy thinks he can fix it but he would like some help on the unit design.
Bob
Dr. Robert R. Wise Department of Biology and Microbiology University of Wisconsin-Oshkosh Oshkosh, WI 54901
(920) 424-3404 tel (920) 424-1101 fax wise-at-uwosh.edu www.uwosh.edu/departments/biology/wise/wise.html
I am a technical recruiter, and I am looking for help with a job opening
for a metallographer on Long Island. Does anyone know a good candidate for my client?
The title of the position is Metallographic Specialist. This individual
will be responsible for maintaining a high technology lab environment, operating the latest lab equipment such as optical image analyzer, SEM, microscopes and hardness tester, interpreting micro sections and coating
structures, and preparing lab reports.
Requirements include a four year technical degree or equivalent experience. Degree in materials science, chemistry, or surface engineering preferred. Should have strong analytical skills and be customer oriented. At least two years experience is needed.
There is never a fee to job candidates for my services. Please call or write for more information:
Pearl Martin Image Associates Inc. 5254 Merrick Road Massapequa, NY 11758 Phone (516)798-3993 Fax (516)797-8703 Email: image-at-worldnet.att.net (preferred) or image-at-nassau.cv.net
I applaud the decision to vent the exhaust. I know of no EPA or other regulation to do so, but the exhaust of mechanical pumps on initial pumpdown is something I have always recommended my customers be rid of. I hope that you are also taking measures to move the pumps into separate rooms or acoustic enclosures to reduce their noise output.
Working with EMs often means spending many hours at a time living with these problems. While occasional exposure can be acceptable, the constant drone of pumps and exposure to pump oil vapors can only be detrimental.
The setup you detail presents no problem to operation of the pumps. Air is very compressable and the volume of air present in the exhaust lines means that there will be only a negligible pressure increase due to the various bends.
A clean-out filter is unnecessary. Proper design of the exhaust stack should provide for a low point where accumulated oil can be either be drained or returned to the pump. This requires less than 90 degree bends in the stack so that any condensed oil can flow back to the pump or a drain. If the exhaust provides for a return to the pump, you have to be aware that there might also be other condensed materials, primarily water, included. Best bet would be to have a slightly greater than 90 degree bend close to the pump followed by a vertical section forming a trap. At that second bend, a drain should be included that would allow for the drainage of all fluids trapped there. Any such scheme would of course require the regular emptying of the trap.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com
-----Original Message----- } From: Lehman, Ann {Ann.Lehman-at-exchange.cc.trincoll.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
VayTek (http://www.vaytek.com ) has been working on something like this. I know of someone who has done it on an SGI computer but I don't know if they have commercialized it yet - I'll try to contact them anad see.
Bill Miller
At 12:58 PM 4/1/99 -0500, Tony Garratt-Reed wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
A Jeol JEM-4000EX high-resolution TEM in my former colleague's lab needs a TV rate imaging or digital imaging camera system. Your information about suitable types of the camera system and related manufacturers or vendors is highly appreciated.
The names of the manufactures or vendors that provide diamond saw or wiresaw for cross-sectional sample preparation are also needed. Thank you in advance!
Cheers,
Jian-Guo Zheng
Dr. Jian-Guo Zheng Materials Science and Engineering Department of Engineering The University of Liverpool Ashton Street Liverpool, L69 3GH United Kingdom Telephone: +44-151-794-4671(office)/5382(lab) Fax: +44-151-794-4675 Email: J.G.Zheng-at-Liverpool.ac.uk
The steps for setting up "good" DIC are simple: 1. Start with setting up Koehler on a well-behaved sample (a cheek cell smear is great for this; make sure that you have the correct thickness coverslip - a number 1 1/2 or 0.17mm thick -- not diameter) 2. If you have separate polarizer and analyzer, cross them so that you get the blackest background possible 3. Insert the DIC prism and tune so that the background is soft gray. This should give you the best 3D impression and contrast.
I wrote a very extensive article on DIC which appeared in the April 1988 (!) issue of American Lab and will send you a Xeroxed copy. There is also a detailed discussion in "Optimizing Light Microscopy for Biological and Clinical Laboratories". Details are available on our website (see signature bar).
Hope this is helpful.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 10:58 AM 4/1/99 -0800, Jon Krupp wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} Terry, } We purchased a new scoring wheel about a year ago from Reichert-Jung (at } the time; heaven knows who owns anybody anymore) for our Knifemaker II } which, I believe is the Reichert update(?) of your LKB Knifemaker. Check } your Reichert-Jung reps for details but I think the parts are all the } same, though the part numbers have changed (probably to protect the } innocent). The details are -- Part 16 706 699 - Scoring Wheel Assembly } Complete. It was simple to install and now works as it did "in the } beginning." Good luck. } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Winston W. Wiggins, Supervisor 01 Apr1999 4:50 PM } CRC-Electron Microscopy Lab Ofc: 704-355-1267 } Carolinas Medical Center Lab: 704-355-7220 } P.O. Box 32861 Fax: 704-355-7648 } Charlotte, NC 28232-2861 USA Eml: WWiggins-at-Carolinas.org } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } -----Original Message----- } From: Terry Robertson [SMTP:terryr-at-cyllene.uwa.edu.au] } Sent: Wednesday, March 31, 1999 6:51 PM } To: 'Microscopy-request' } Subject: Glass Scoring Wheel for LKB 2178 Knife Maker II } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Does anyone involved in Electron Microscopy know where I can obtain spare } glass scoring wheels for an LKB 2178 Knife Maker II. Any helpful advise } would be welcome as our machine requires a replacement scorer ASAP. } } Dr Terry Robertson (PhD) } Senior Research Fellow } Department of Pathology } University of Western Australia } Nedlands 6907 } } Phone 618 9346 2935 } Fax 618 9346 2891 } Mobile phone 618 0415 986531 } email terryr-at-cyllene.uwa.edu.au } }
South Bay Technology produces both wire saws and diamond wheel saws for T= EM cross section preparation (among many other uses). You can see additiona= l information on all of our products on our web site at:
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You may also have an interest in our new Ion Milling System which feature= s patented, new Low Energy Gun Technology (LEG). Please contact me off-lin= e for more information.
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Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy. Message text written by "Dr J. G. Zheng" } ----------------------------------------------------------------------- The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
Dear All,
A Jeol JEM-4000EX high-resolution TEM in my former colleague's lab needs = a TV rate imaging or digital imaging camera system. Your information about suitable types of the camera system and related manufacturers or vendors = is highly appreciated.
The names of the manufactures or vendors that provide diamond saw or wiresaw for cross-sectional sample preparation are also needed. Thank you=
There is an immediate opening for an experienced Scanning Electron Microscopy (SEM) Research Technician at Exxon Research and Engineering Company's Corporate Research Laboratory in Clinton, New Jersey. The position initially is on a temporary basis driven by an immediate need. Initial duration of the position is 4 to 12 months.
As a member of the Advanced Characterization Group at Corporate Research, this position will involve the operation of the state-of-the-art JEOL FESEM, and JEOL Analytical SEM instruments with associated Energy Dispersive and Wavelength Dispersive Spectrometers. The position will involve the creative application of high resolution SEM imaging and elemental characterization of samples related to a wide range of projects at Exxon's Corporate Research Laboratory. The position will also involve general laboratory operations, sample preparation, SEM maintenance and computer analysis of the SEM data (both image analysis and spectral analysis).
Previous SEM experience and experience with high vacuum systems and computers (DOS, Windows, and Unix) is required. Successful candidates should have experience in chemistry, physics or material science with a Baccalaureate degree or equivalent experience. Since a number of projects are simultaneously in progress, it is essential for the researcher to be very organized and to pay attention to detail and accuracy in reporting results.
Qualified candidates please send resume to:
Human Resources Recruiting - RTSEM Exxon Research and Engineering Company P.O. Box 998 Annandale, New Jersey 08801-3344
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Being the Diaplan Microscope utilized the individual objective prisims it is not a easy task tuning to achieve the preferred DIC image. To do the tunning, you must loosen the three little set screws on the 10mm collar located above the objective. Then with polarizers crossed and the matching condenser prisim in place you should rotate the objective to achieve the three demensional appearance. The best position will allow to have an even flatfield image throughout the entire field. If this does not appear to be the case then you do not have the correct orientations. You can also remove the eyepiect and look down the tube to see an interference patter. Typically when you are aligned the pattern which appears as a black line should be orientated on a forty five degree angle from two oclock to eight oclock. Once you have these prisims orientated be sure to lock the little set screw on the objective. FYI this setup with objective and prisim together will not allow you to use the same optics for typical brightfield applications should that be desired. Usually back then because of the nature of the beast (Diaplan) you had to have a interchangeable nosepiece when going from one illumination method to another. With todays infinity corrected systems and the technological developments in prisims it is easy to use a microsscope with all the latest gizmos and not have to take anything off the unit.
If you would like, I have a old instruction manual called "Interference Contrast T for the Leitz Diaplan". I would be more then happy to copy it sometime this week and send it along.
Let me know... Good Luck!
Also, a good sample to use is a section of unstained tissue about five microns or less. If you have access to someone working in a clinical pathology lab you may want to ask them.. to get this for you..I may have one or two laying around. If I do I will send it along with the manual.
C. Passione -----Original Message----- } From: Jon Krupp {jmkrupp-at-cats.ucsc.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
You should not have to go through all this trouble! Leitz/Leica have a great reputation for quality and those prisms should have been aligned "at the factory".
The tuning I was referring to was the compensator which adjusts the "pol color" in the background.
Also, I am surprised to hear that you could not pull out the prism/compensator from the back focal plane of the objective and move the condenser to the brightfield setting to re-establish good imaging for brightfield.
Jan Hinsch, if you are listening .... any comments?
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 07:21 AM 4/3/99 -0500, Cono Passione wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I beleive the previous Leica/Leitz microscopes of the fixed focal length vintage almost alway utilized individual prisims that were permanent unless you unscrewed the objective then physically remove the 10mm prisim. You then would be able to put the objective back in place on the nosepiec to utilize its BF capabilities. To return to the DIC mode you would have to put the DIC prisim back in place and realign over again. The prisim in place trying to do BF techniques will be distorted and very difficult to resolve anything. This is from past experience. If someone else has developed a system to remove the prisims on a slider type set the Diaplan or laborlux series I would be intereset in knowing more about it. I also beleive the older Zeiss mciroscopes utilized an individual prisim that was pused in and out on a forty five degree angle right above the objective being used at any particular time. Some of these prisims were capable of matching up with Leitz objectives with various combinations.
Thank You
C. Passione -----Original Message----- } From: Barbara Foster {mme-at-map.com} To: Cono Passione {iami-at-nauticom.net} ; Microscopy-at-Sparc5.Microscopy.Com {Microscopy-at-Sparc5.Microscopy.Com} ; Jon Krupp {jmkrupp-at-cats.ucsc.edu}
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Kun Li wrote the following: =================================================== We are trying to study the microstructure of cold drawn 25 µm gold wires. We meet the difficulty in etching the gold wires to reveal the grain structure in SEM and optical observations. Could somebody give us some suggestions on how to etch the wires. =================================================== While many laboratories have the capability, very few encounter the kinds of samples that would actually benefit from the application of "sputter etching". This is the process of reversing the polarity of a normal gold (sputter) coater, or having it operate in "etch" mode, whereby instead of sputtering gold onto the sample, you are sputtering (in this case, gold) off of the sample. Assuming you are working with polished cross-sections (but the outside surface of the wires should be able to be studied this way as well), you should be able to end up revealing details of the microstructure.
We have not done this in-house ourselves but we have been told by others they use this approach, for example, on gold alloys used in dentistry.
The second approach is to do this by reactive plasma etching. It is a different physics so the effect should not be expected to be the same. Again we have not done this ourselves in-house, but we have customers who claim to have done it. I am not real clear what would be the optimum etching gas. We ourselves found that we could eventually remove gold from a gold coated SEM sample using only oxygen (for 20 minutes). Others have used Ar and CF4. I have myself never quite been able to understand why oxygen would remove the gold layer from a gold coated SEM sample.
Is one approach "better" than the other approach? I don't know. What we would predict is that if the two approaches were compared, sputter etching vs. reactive plasma etching, there should be differences. But then again, the physics is different when comparing the two approaches so this should not be surprising.
Finally you might wonder about why chemical etching would not be suggested for this kind of application. I am not sure whether it would or would not work, but when I have heard people discussing doing it, they seem to be referencing some pretty terrible and nasty reagents, the main one being aqua regia. Therefore the interest in alternatives to wet chemical etching.
Disclaimer: SPI Supplies manufactures both sputter coaters with etch and reactive plasma etchers and we therefore have a vested interest in seeing these techniques used more widely. There is nothing "special" about the SPI etch mode, the same physics would be at work in anyone's sputter coater so long as it has the ability to have its polarity reversed and operate in "etch" mode.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
I am looking for a used Zeiss 10C. Please let me know if you have one for sale or if you know of anybody who does. Thank you very much. Peter Jordan, EMSI
I'd like to get from somewhere or somebody a manual on SEM Hitachi S-4100 in English. Copying and shipment charge is definitely to be compensated.
Dmitri. P.S. Please, send me at least some ideas, where to get it from!
__________________________________________ Dmitri V. Sokolov, Doctor Course student Research Center for Interface Quantum Electronics, Hokkaido University, North 13, West 8, Kitaku, Sapporo 060, Hokkaido, Japan Phone 81-11-706-7174 Fax 81-11-716-6004 http://www.geocities.com/SiliconValley/Campus/1314 AOL Instant Messenger FalconDot ICQ 9418072 __________________________________________
As sometimes happens, some people find they can't get money to attend courses at the last minute while others don't hear about them until it is too late to apply.
Now may be your chance!
Three late cancellations at the UBC Live-Cell course mean three opportunities for those of you who have ten days free this June.
We expect to have an international faculty of 15, and on-site equipment that includes 2-photon systems from Olympus (and maybe Leica). There will also be single-photon confocals from Bio-Rad, Olympus, Noran, Nikon, Zeiss and Yokogawa/EG&G, deconvolution set-ups from API, AutoQuant/Universal-Imaging, Improvision, Intelligent-Imaging and Vaytek all set up for "live-cell" work. In addition, there will be was laser tweezers, microinjection and sundry other delights.
These systems are not just to look at but to use for over 45 hours of organized 2D and 3D live-cell laboratory sessions, plus 20 hours of evening sessions for group live-cell projects.
Although the eight "Groups-of-3" have already been set up and have chosen their "individual projects," we are able to accept 3-2 more students as long as their backgrounds will fit into one of these groups.
which has the rest of the story, including a preliminary program.
Then fill out the Registration Form from the site to tell us about you, Fax it to me ASAP and we will see if we can fit you in. (I will be away the week of April 9-18)
Hope that you can join us in Vancouver this June 16-27.
Jim Pawley, Organizer **************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
I am looking for a used carbon coater for our SEM teaching lab. If you have one for sale or if you know of anybody who does, please let me know. Thanks!
Mei-Zhen Dang Research Associate Department of Physics University of Ottawa
We use the Hitachi S4100 on many occasions during our "in house" courses.= =
We do not have the Hitachi manual but we have developed a "quick guide" t= o the instrument for our clients, that I can e-mail as an attachment if yo= u wish? The trainers also use a mechanical alignment guide that we produce= , that is also available to you if you wish?
Sorry I cannot act today but with ester and all that lawns to cut etc.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
This young man has contacted me twice in regards to interviewing a person involved in nanotechnology. If you can help please respond to him directly with the answers to the questions at the bottom of the page.
Mr. Nano {nanoteknologist-at-hotmail.com}
Thanks
Susanne Pignolet Brandom MicroWorld 978-456-3100
} Hi, my name is Michael Lee, and I am a high school student attending } Bellevue High. I am doing a project about a career in nanotechnology. } As part of the project, I need to interview someone with general } questions about a career in this field. I was wondering if you would be } able to help me by letting me interview you, or could refer me to } someone who I would be able to interview. If I would be } able to interview you, please let me know what type of contact would be } most convenient for you (email, phone, etc). I would really appreciate } it, thank you. } } -Michael Lee
-----Original Message----- } From: Mr. Nano {nanoteknologist-at-hotmail.com} To: spb-at-mwrn.com {spb-at-mwrn.com}
} ---------- } From: Earnhart, James P. } Sent: Monday, April 05, 1999 7:25 AM } To: Ingber, Bruce F. } Subject: RE: Mech Pump Discharge Backpressure } } Only to say that if the pumps were designed to be able to } operate in the described manner then the manufacturers would have specs on } how and what type of ventilation system to install under different } applications, i.e. extraction type if there is more than 10' of pipe to } outside, or more than 2 bends totaling more than 90 degrees. Also, if } discharging the "bad air" outside then EPA standards must be met...such as } installing "scrubber" systems to insure no oil or certain hydrocarbons are } discharged into the air and eventually being introduced into the ground } water. Best thing is to install a factory offered oil recovery exhaust } filter system such as the one we spoke about on the coating system. And } to check with OSHA as to the "hazards Imposed" by the Vacuum Pump vapors. } If they say they are too hazardous for normal laboratory environments then } we may explore the possibility of routing the exhaust into existing "Fume } Hoods" in the laboratories. That is assuming they have the necessary } environmental "cleaning" systems built into them. } } Bottom line is I doubt that without a lot of engineering the } pumps will be able to be operated properly with the exhaust "routed" } through any type of piping more than a few feet long. Meaning that if you } hook any lines, more than a few feet long without bends or any that have } more than 90 degrees of bends, to remove the fumes to any of the pumps, } they won't work properly without a lot of engineering to eliminate any } "back pressure" that may be caused. } } ---------- } From: Ingber, Bruce F. } Sent: Thursday, April 01, 1999 3:43 PM } To: Earnhart, James P., Electronic Technician } Subject: FW: Mech Pump Discharge Backpressure } } Any ideas? } Bruce F. Ingber Biologist- Electron Microscopy USDA-ARS, SRRC 1100 Robert E. Lee Blvd. New Orleans, LA 70124-4305
} ---------- } From: Lehman, } Ann[SMTP:Ann.Lehman-at-exchange.cc.trincoll.edu] } Sent: Thursday, April 01, 1999 9:56 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: Mech Pump Discharge Backpressure } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } Dear Listers, } } My Safety Officer wants all our mechanical pumps } vented outdoors. The pumps } are Sargent-Welch, Alcatel, and Edwards and are used } to back TEMs, film } desiccators, evaporators, etc. Initially I thought } this was a good idea, } having done it simply in past lives by drilling a } hole in a wall, ganging } the outlet hoses, and presto - out the bad smoke } went. } } However the B&G engineering consultant got hold of } the project (yes now it's } a 'project') and now EVERY pump must get its own } line up to the roof. This } means each pump's discharge is directed to a run of } 1-inch copper pipe with } three to five 90-degree bends over a total vertical } length of about 20'. } (Luckily I am on the top floor.) There is also talk } about inserting a } clean-out or filter at each feed-through for dealing } with accumulated oil. } } I'm concerned that the pumps are not designed for } backpressure on the outlet } side coming from the 20' column of air plus the } resistence from the } 90-degree bends and filter. } } Could this setup affect the (1) efficiency or (2) } overall life of the pumps? } Am I being overly cautious? } } I'd appreciate feedback from the List (including } manufacturers) re the } feasiblity of this approach, and the pump specs - I } haven't been able to } find anything about discharge 'load' tolerances. } } Thanks, you can reply offline and if there is } sufficient interest I'll } summarize responses for the List. } } Ann Hein Lehman } EM Facility Mgr } Trinity College } Hartford, CT 06106 } v. 860-297-4289 } f. 860-297-2538 } e. ann.lehman-at-exchange.cc.trincoll.edu } } }
} I applaud the decision to vent the exhaust... } While occasional exposure can be acceptable, the constant drone } of pumps and exposure to pump oil vapors can only be detrimental. } Not to mention that droplets of condensed oil vapor could contami- nate the specimens. Yours, Bill Tivol
One of our students has been given the use of a Model ZF Coulter counter but we cannot find the conversion chart which we have been told is necessary to have, to convert the machine readings to a cell count. Does anyone on the list have this same machine and could you perhaps FAX the chart to us at 617-638-5591? We are located in Boston, MA. Are instructions for this device hard to come by?
* Subject: Wanted Leitz Parts...... * Date: Mon, 05 Apr 1999 09:34:37 -0400 * From: Joseph Passero {jp-at-spacelab.net} * To: Microscopy-at-Sparc5.Microscopy.Com * * I am looking for the following Leitz parts for use with the Zernike * 402a phase contrast condenser. * * An auxiliary focusing (magnifier) with the knurled ring for focusing on * the phase ring, Leitz part number 513 123 * * A pair of centering key for the annular stops of the condenser. * * PHACO objectives * * If you have these or any other Leitz items you would like to * sell, please tell me what you have with a price. * * Thank You * Joseph Passero * jp-at-spacelab.net
-----Original Message----- } From: Ingber, Bruce F. {bingber-at-commserver.srrc.usda.gov}
} } Only to say that if the pumps were designed to be able to } } operate in the described manner then the manufacturers would have specs on } } how and what type of ventilation system to install under different } } applications, i.e. extraction type if there is more than 10' of pipe to } } outside, or more than 2 bends totaling more than 90 degrees. Also, if } } discharging the "bad air" outside then EPA standards must be met...such as } } installing "scrubber" systems to insure no oil or certain hydrocarbons are } } discharged into the air and eventually being introduced into the ground } } water. Best thing is to install a factory offered oil recovery exhaust } } filter system such as the one we spoke about on the coating system. And } } to check with OSHA as to the "hazards Imposed" by the Vacuum Pump vapors. } } If they say they are too hazardous for normal laboratory environments then } } we may explore the possibility of routing the exhaust into existing "Fume } } Hoods" in the laboratories. That is assuming they have the necessary } } environmental "cleaning" systems built into them.
If you are worried about back pressure you can put a fan in the lines to put negitive pressure at the outlet of the vacuum pumps.
Gordon
Gordon Couger gcouger-at-couger.com www.couger.com/gcouger Stillwater, OK 405 624-2855 GMT -6:00
A few months ago I posted a request for a used SEM to purchase for a customer of mine and I was grateful for the range of replies I received. We managed to find an instrument that I felt was a perfect fit for their needs and that was within their budget, while the university that was selling it got the price they wanted and a quick sale. Thanks to all who helped along the way.
Well, I've got another request from our neighbors south of the border. A pathology lab is being setup from scratch, and one of the requirements is for an SEM, no mention of an EDS or WDS system. While they would like a later model instrument, their needs are for a basic SEM. Any and all replies are welcome, please email the specifics to me, I'll compile them and present the responsible parties with the data. While I'm not likely to travel again to Mexico to install and service an SEM (did it once before - long trip, language problems), I may come and deinstall the instrument.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com
Thanks to those who responded with information on upgrading the computer on our BioRad Confocal 600 with COMOS
Our new computer is 8gig HD with CD, zip, internet card and all the bells and whistles. Apparently there is a limitation with the old program having trouble with hard drives that are too big, so we partitioned ours. Otherwise, it appears to have no trouble accessing any of the drives. We have a Panasonic WORM, that we are setting up on the old computer as a separate station - along with a 5 1/4, 3 1/2, and Zip so that whatever walks in the door can be transferred to a more recent fad of data storage. We try to keep a lot of older equipment/computer technology around and working so others can retrieve their data after they've suddenly been aroused from dormancy. e.g. we had a researcher come in with data stored on a WORM drive (she was working with the government) and we were the only ones she found(she states)with one still in service. Similar story with the 5.25 drive.
Now, refurbishing the detector head and laser.... Oh boy, I can hardly wait.
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
DearListers, I have a GATAN (model#613) single tilt , LN cooled side entry cold stage for a Phillips 300 or 400 TEM available. I'm inquiring to see if there is any interest in purchasing it? Rosemary
Hi everyone, I'm training to be a tech here in SEM and Confocal microscopy. I am in need of a method to clean a leaf from an ocean plant to then be viewed with SEM. Any help for this rookie would be greatly appreicated. Thanks Lee Ann Baldridge SEM &Confocal Facility IUSD Indpl., IN lhadley-at-iusd.iupui.edu 317-274-5142
Our lab is looking to buy a belt grinder for heavy grinding of mounted cross-sections (metallographic cross-sections). The one quote I got was for over $5,000 for a single belt with coolant system (1/3 hp motor). The dual belt system was almost $9,000. This seems quite pricey for what you get. Is there any other options available to us?
Mark
---------------------------------------- Mark C. Biesinger, Research Scientist Surface Science Western The University of Western Ontario London, Ontario, Canada Tel: (519) 661-2173, Fax: (519) 661-3709 http://www.uwo.ca/ssw/
Members of the list: I seem to remember that a company existed that made disposable microtome knives. The knives were about the size of a regular knife, only thinner. The company seemed to go by something like "American Scientific Products." Is this company still in business, or are there satisfactory substitutes for students doing basic slide making?
George W. Smith
********************************* George W. Smith, Ph.D. Associate Professor of Biology Department of Biological Sciences Union College Schenectady, NY 12308 (518)388-6245 smithg-at-union.edu *********************************
Dear Users, Someone sent me a message asking for help using Molecular Probes Alexa dye reagents. I no longer have this message as our email file server crashed before I could respond and several days messages were lost. Recovery of damaged disk is underway. Would the person who asked about this please re-send their message. However, I would like to know more of the specifics of your problem. We have had no problem substituting these reagents for like reagents in a variety of cell type systems and tissues?
Images & Info at http://www.molbio.princeton.edu/confocal {http://www.molbio.princeton.edu/confocal}
Joe Goodhouse Confocal / EM Core Laboratory Department of Molecular Biology Princeton University jgoodhouse-at-molbio.princeton.edu {mailto:Jgoodhouse-at-molbio.princeton.edu}
I believe the item you are looking for was made by Scienctific Products; they were item # M7200 and came 12 to the box. A quick scan of the catalog didn't show them anymore, but maybe they are still around. I have several old (from the '70's) boxes of of them you are welcome to have if you wish. For my Histology class, I use instead special holders for single edge razor blades; cheap, easy, no resharpening and most importantly, very short exposed edge to prevent bloody knuckles. I'm not sure where they come from, but if you are interested I will try to find the source.
For a project on cartilage regeneration in dogs, I would like to be able to determine the origin of the new cartilage cells. I know that one way would be a classic pulse-chase experiment with tritiated thymidine to label dividing cells, but I'm wondering whether there might non-radioactive methods that could tell me the same thing. I'd prefer not to have to inject radioactive solutions (even tritium) into these animals. Any ideas?
Gary Radice 804-289-8107 Department of Biology 804-289-8233 (FAX) University of Richmond gradice-at-richmond.edu Richmond VA 23173
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After reading the excellent ideas already presented about this subject, it occurred to me that there may be one other method that would work. First, it is no longer necessary to use things like aqua regia to polish gold. My paper in Ultamicroscopy 25 (1988) 351-354 describes an electropolish for gold using certain chemicals dissolved in methanol and butyl cellosolve at -50 degrees C. It is often possible to develop a nice etch by simply using an electropolishing solution at a lower voltage. In this case I would suggest dropping the voltage from 150 to perhaps 70 volts. A temperature of 0 C. may also be cold enough. The good thing about this is that by controlling the time the current is "on", the amount of etching can be controlled. The electrolyte does not attack gold when the current is off. This solution is easy to rinse off the specimen.
Bernard Kestel Materials Science Division Argonne National Laboratory Argonne, Il., 60439
for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 7 Apr 1999 07:49:02 -0700 (PDT) X-SMTP: helo gaugler.calweb.com from gaugler-at-calweb.com server -at-gaugler.calweb.com ip 207.173.132.32 user=Pgauglr Message-Id: {4.1.19990407073131.009d1100-at-pop.calweb.com} X-Sender: gaugler-at-pop.calweb.com X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1
I would appreciate hearing from and about independent companies or individuals who take down, move and set up SEMs. Specifically, I would like to receive quotes for moving an Amray 1830 from TX to Sacramento CA. This would involve disconnecting the system, locking down the turbo pump, crating, moving, and re-install at my location.
I have a quote from Amray but they do not handle moving. I am wondering if this is standard practice or if there are reputable folks who can handle the whole job from start to finish via one contact. I would anticipate that someone who knows about this specific SEM model would be preferred.
Anticipated timeframe for start of project is in about 1-2 months from now.
Dear George, Several suppliers of disposable microtome knives come to mind:
Electron Microscopy Sciences 321 Morris Road Ft. Washington, PA 19034 1-(800) 523-5874 (web site address: http://www.emsdiasum.com.)
C.L. Sturkey, Inc. 824 Cumberland Street Lebanon, PA 17042 1-(800) 274-9446 FAX (717) 274-9442
Sturkey offers free samples of their knives. Our experience in recent years has been with Sturkey knives, but other brands may be prefered depending on your application. Best regards, Henry **************************************************************************** Disclaimer: I have no vested interest in the firms mentioned above. ****************************************************************************
Henry Eichelberger, EM Facility Manager Department of Biological Sciences Binghamton, University
Try C.L. Sturkey... They should be on the net......
C YA -----Original Message----- } From: George Smith {smithg-at-union.edu} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
I would just like to support and reinforce some of the many comments recently made about getting copies of older instruction manuals. It would be a real service to our community if a master compilation of these manuals, with a suitable index and regular updates, could be put together. I wish that I had the time and the resources to volunteer for this task but I don't at the moment. Hope somebody else does.
I am trying to lift the carbon coating off a metallic film. The C is less than 10nm thick. My best results so far involved aqua regia vapors, then dipping the sample in DI water, and finally picking up the C on grids as it floats on the water. However, one of the components of the metal film under the carbon is Pt, and a large number of small Pt particles stay on the carbon. Could someone recommend a Pt etch?
} Job Description } There is a TEM microscopist position open for a materials scientist at } Intel Malaysia. The primary job responsibility is to provide technical } consultation and leadership in applying materials/surface analysis & } microscopy techniques to solve day-to-day engineering and manufacturing } problems, including operation of a JEOL 2010F TEM. This individual is also } expected to develop and proliferate materials & surface analysis } capabilites within Intel Malaysia; to recommend and set up new analytical } techniques; and to coach & mentor junior-level failure analysis engineers. } } Requirements } Candidates should have a Ph.D. in Materials Science, Surface Science, } Physics, Chemistry, Thin Film Engineering or equivalent. Successful } candidates will have a strong background in microelectronic materials & } process engineering (silicon and/or packaging materials). You will have } studied and applied advanced bulk, thin film and surface characterization } techniques intensively to problems relevant to microelectronics } processing, packaging and/or failure analysis during your graduate } education and/or research/industrial experience. You should have extensive } hands-on TEM experience. Knowledge & hands-on experience in applying XPS } & TOF-SIMS in analyzing organic contaminants would be an added advantage. } } You should be highly motivated, self-directed, effective working } independently or in a team. Good problem solving skills, interpersonal, } verbal & written communication skills are necessary. Must be able to } impart your knowledge/skill to junior-level engineers. } } Interested individuals please contact: } Kian Sin Sim } Intel Technology Sdn. Bhd. } 11900 Penang, Malaysia. } Tel: ++ 604-859-6477 } Fax: ++ 604-859-6749 } kian.sin.sim-at-intel.com
Hi, We need to replace the stereo scope on a Reichert-Jung Ultracut E (purchased in 1986, Type 70-17-04, Fabr NR 396313). Something is messed up in the lens system making it impossible to focus. We sent it to Leica to be repaired and they couldn't fix it. I'd like to hear from you if you have a stereo scope for sale that would work on this microtome. The stereo scope label says: Stereo Star ZOOM Reichert 0.7X TO 4.2X 570
thanks, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
We are a training company that has been operating for the past 18 years teaching SEM, TEM and EDX in many of the English speaking countries of th= e world. As part of our "in house" course procedures we have produced our own 1 to 4 page instruction sheets to help our clients. These "quick guides" now number well in excess of 100, even with some of them covering= a range of microscopes with almost identical operating panels. This means = we have data going back to instruments produced in the late 80's which, provided the demand is not too excessive, we are pleased to offer via attachments to e-mail. We also have basic maintenance procedures for man= y models.
Hope this may help those who are in trouble with the older instrumentatio= n?
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
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Hi,
can somebody help me with a protocol for embedding plant material in LR white. The material is fixed in PFA and kept in 70% EtOH at -20 C.
Bo
-- Dr. Bo Johansen Associate Professor Botanical Institute University of Copenhagen Gothersgade 140 DK-1123 Copenhagen K Denmark
Is there a quantitative method for determining the metal grain size when metal shadowing a biological TEM specimen?
Shadowing is new to me and I'd like to find the best conditions for TaW shadowing in our shadowing unit. To that end, I was wondering if there was something more quantitative than eyeballing TEM images of the shadowed samples?
Hello everyone. I would like to know if there is a quick write-up on the = web that explains how to prepare thin sections of rocks and sand for = microscopy. I need to know specifically what type of epoxy is used to = affix the specimen to the microscope slide. Thanks.
______________________ Roberto Garcia Senior Analyst, Metallography North Carolina State University Analytical Instrumentation Facility Box 7531, Room 303 EGRC Raleigh, NC 27695-7531 rgarcia-at-unity.ncsu.com http://spm.aif.ncsu.edu/aif
{!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"} {HTML} {HEAD} {META content=3Dtext/html;charset=3Diso-8859-1 = http-equiv=3DContent-Type} {STYLE} {/STYLE}
{META content=3D'"MSHTML 5.00.0910.1309"' name=3DGENERATOR} {/HEAD} {BODY bgColor=3D#ffffff} {DIV} {FONT size=3D2} Hello everyone. I would like to know if there is a = quick=20 write-up on the web that explains how to prepare thin sections of rocks = and sand=20 for microscopy. I need to know specifically what type of epoxy is used = to affix=20 the specimen to the microscope slide. Thanks. {/FONT} {/DIV} {DIV} {/DIV} {DIV} {FONT size=3D3} ______________________ {BR} Roberto Garcia {BR} Senior = Analyst,=20 Metallography {BR} North Carolina State University {BR} Analytical = Instrumentation=20 Facility {BR} Box 7531, Room 303 EGRC {BR} Raleigh, NC 27695-7531 {BR} {A=20 href=3D"mailto:rgarcia-at-unity.ncsu.com"} rgarcia-at-unity.ncsu.com {/A} {BR} {A=20 href=3D"http://spm.aif.ncsu.edu/aif"} http://spm.aif.ncsu.edu/aif {/A} {/FON= T} {/DIV} {/BODY} {/HTML}
I've called their number (973) 882 6611 to confirm, and it appears true that Coolwell has folded up. However, one can still leave a voice mail message at that number for Frank Haze, and presumably he can be helpful.
We have an early analog SE-111A, which we have substantially modified as various parts have malfunctioned or worn out. Despite its variable reliability, in my opinion, it's still the best chiller design out there, and our unit easily keeps the STEM coolant temperature within 0.10 of 700F.
I have the operating manual as well as the "pilot circuit" and the "power circuit" diagrams; however, Coolwell has refused to release the refrigeration system diagram, treating it as a trade secret. I will be glad to scan these and e-mail them to any interested party. Please, direct your requests to:
rwafu-at-bsco.com Valdemar Furdanowicz Bethlehem Steel Research Labs G-165 Bethlehem, PA 18016
-----Original Message----- } From: Bob Wise [mailto:wise-at-vaxa.cis.uwosh.edu] Sent: Thursday, April 01, 1999 6:35 PM To: Microscopy-at-sparc5.microscopy.com
To all,
Our Coolwell "SE-Style" Chiller hooked to our TEM is on the fritz. An answering machine at the Coolwell phone number says they went out of business and refers one to Litron (or Lintron?). A call to them reveals that all they bought from Coolwell was their name and "marketing strategy". Apparently the marketing strategy is to not produce replacement parts for Coolwell chillers. So we are on our own. Does anyone have a wiring diagram, schematics, specs (such as type and amount of coolant), and/or advice for a SE Style Chiller they could share with me? Our campus refrigeration guy thinks he can fix it but he would like some help on the unit design.
Bob
Dr. Robert R. Wise Department of Biology and Microbiology University of Wisconsin-Oshkosh Oshkosh, WI 54901
(920) 424-3404 tel (920) 424-1101 fax wise-at-uwosh.edu www.uwosh.edu/departments/biology/wise/wise.html
} From Microscopy-request-at-sparc5.microscopy.com Thu Apr 8 13:57:42 1999 } } From: "Roberto Garcia" {rgarcia-at-unity.ncsu.edu} } To: "MSA Microscopy" {Microscopy-at-Sparc5.Microscopy.Com} } Subject: Geologic Thin Specimen } Date: Thu, 8 Apr 1999 11:10:01 -0400 } } } Hello everyone. I would like to know if there is a quick write-up on the } web that explains how to prepare thin sections of rocks and sand for = } microscopy. I need to know specifically what type of epoxy is used to = } affix the specimen to the microscope slide. Thanks. } } ______________________ } Roberto Garcia } Senior Analyst, Metallography } North Carolina State University } Analytical Instrumentation Facility } Box 7531, Room 303 EGRC } Raleigh, NC 27695-7531 } rgarcia-at-unity.ncsu.com } http://spm.aif.ncsu.edu/aif } } Robert, We have had good success with Epotek 301 (Epoxy Technology, Inc. 978-667-3805) for both thin sections and thick sections (slabs) for cathodoluminescence studies. With cathodoluminescence it is important that the epoxy can stand up reasonably well under the electron beam bombardment if it is hit directly (e.g., in a void) and also that it not outgas excessively and harm the vacuum.
Don Marshall
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology "A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)
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We would like to label artificial Xenopus oocyte nuclei after sticking them on glass. So far, we tried to centrifuge the nuclei to a glass cover slip, fix it with formaldehyde, briefly extract with triton, label them and flat embed in Epon. The problem is they do not stick very well. So far, we tested naked glass, polylysine coated glass and carbon coated/glow-discharged glass. After the centrifugation, there is usually plenty of nuclei, but with the processing, we are loosing many if not all.
Any suggestions would be greatly appreciated.
Regards,
-- Michal Jarnik, Ph.D. Fox Chase Cancer Center Electron Microscopy Facility 7701 Burholme Ave. Philadelphia PA 19111 Tel. 215-728-5675 Fax 215-728-2412
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} We would like to label artificial Xenopus oocyte nuclei after sticking } them on glass. So far, we tried to centrifuge the nuclei to a glass } cover slip, fix it with formaldehyde, briefly extract with triton, label } them and flat embed in Epon. The problem is they do not stick very well. } So far, we tested naked glass, polylysine coated glass and carbon } coated/glow-discharged glass. After the centrifugation, there is usually } plenty of nuclei, but with the processing, we are loosing many if not } all. } } Any suggestions would be greatly appreciated.
You might try "silane", 3-aminopropyltriethoxysilane to be precise. Get it from Sigma (cat. no. A-3648). 1. Wash slides thoroughly with hot, soapy water. Rinse very well, final 2 rinses with distilled. water. 2. Dry in an oven. 3. Dip slides in 2% silane in actone for 10 sec., then 2 rinses of acetone, 1 min. each. 4. Air dry in a vertical position.
You might also take a look at Stain Technology 62: 27-33 and 93-99, 1987. Two very interesting papers by S. Fink.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
Just testing yet another filter I've configured. This one is supposed reject all Email which has attachments. This is the most common way that virus's are transmitted by Email as attached documents.
Of course, NONE of you attach documents to postings on the Microscopy Listserver right? After all that is part of our rules.
Remember VCF cards that some WWW browsers attach to your Email are also attachments so you had better make sure your browser is configured so as not to attach those annoyances.
} Mime-Version: 1.0 } Date: Thu, 8 Apr 1999 17:59:16 -0600 } To: microscopy-at-Sparc5.Microscopy.Com } From: "Nestor J. Zaluzec" {zaluzec-at-Sparc5.Microscopy.Com} } Subject: Administrivia: Testing Virus Filter } } Colleagues.... } } Just testing yet another filter I've configured. This one is supposed } reject all Email which has attachments. This is the most } common way that virus's are transmitted by Email as attached } documents. } } Of course, NONE of you attach documents to postings on the } Microscopy Listserver right? After all that is part of our } rules. } } Remember VCF cards that some WWW browsers attach to } your Email are also attachments so you had better make } sure your browser is configured so as not to attach those } annoyances. } } } Nestor } } }
Just testing yet another filter I've configured. This one is supposed reject all Email which has attachments. This is the most common way that virus's are transmitted by Email as attached documents.
Of course, NONE of you attach documents to postings on the Microscopy Listserver right? After all that is part of our rules.
Remember VCF cards that some WWW browsers attach to your Email are also attachments so you had better make sure your browser is configured so as not to attach those annoyances.
It's taken me a while, but I think that I now have a running version of the Email attachment filter I was working on last week, a few of you will recall a batch of rejected mail messages on 3/30. which was part of my early testing.
Local testing is now completed and I have put this filter on-line as of ~ 7 pm CST on the Full Microscopy Listserver. Please bear with me as I'm sure there will be a few glitches along the way. Especially the first few days of full operation.
This filter scans each Email message for an attachment. If an attachment is found (and I can't guarantee the filter will catch all of them) then the Email posting will be REJECTED and an explainatory message is sent to the poster. Attachments are one of the most common way that computer virus's are transmitted by Email especially they are embedded in various documents. Removing the attachment will generally allow your message through, unless of course your Email triggers yet another different "warning flag".
Of course, NONE of you attach documents to postings on the Microscopy Listserver right? After all that is part of our rules which you all have received on subscription and have read completely. The only attachments we will see are from JUNK mailers...right?
Just a final warning, you should all appreciate the fact that VCF cards that some WWW browsers "append" to Email messages are also "attachments". The filter does not differentiate attachments. So I run the risk of getting a handful of you annoyed at me. Obviously the simple solution is for those of you who may be affected to reconfigure your browser/mailer not to send VCF cards. (Netscape is particuliarly bad in this regard) so WWW users beware!
In the long run, I think it is better to protect the larger group of you and catch the grief I will get from those few who don't realize that VCF cards are attachments and consider my rejection of their mail an afront on their "virtual" personality. .......
Cheers.... Nestor Your Friendly Neighborhood SysOp.
----------------------------------------
PS. Just for those of you that are curious the filter has logged 255 messages as potential SPAM/JUNK mail since it was started August of 98.
In other words about 8 message per week trigger the filter. About 3/4 of those caught are true JUNK mail. It also inadvertently catches a few valid postings which after the author contacts me as per the instructions the messages are allowed through, albeit a day or so later.
Right...... time for a cold beer and some dinner. G'night all
Ritchie Sims wrote: } } } Date: Wed, 07 Apr 1999 15:54:35 -0700 } Hi, Earl } } Just off the top of your head, how much would you guess that a } competent person might be able to disassemble a JEOL 840 in a US } city, and crate it suitably for international despatch? } } Just to help my budgeting } } cheers } } Ritchie } } } Hi all, } } } } There are a number of qualified technicians, some of whom have worked } } for Amray that can handle this type of job. The Amray 1830 is a } } relatively simple SEM requiring about 8 hours maximum to dis-assemble } } and about 16 hours to re-assemble. I don't recommend crating the system. } } Instead, we usually ship via "padded air-ride van". Shipping costs are } } charged according to weight and distance. Maximum costs for shipping and } } SEM coast to coast has been about $3,000.00. Shipping costs from Texas } } to Calfornia should be considerably less. } } } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand
Hi Ritchie,
A Jeol 840 has quite a bit more cables. It would take a good tech about 12 hours (max) to properly dis-assemble and then another four hours to supervise the crating. The costs for crating is about $800.00 USD. The labor for 16 hours is about $2000.00 USD plus travel time & travel expenses.
Actually, a rather complicated subject. In regards to your overall question, anyone is capable of arranging the shipping. That is a simple matter. Many independents, however, may be reluctant to accept the responsibility. If something goes wrong, you might end up with multiple lawsuits - you sue the contractor, who sues the shipper, who counter-sues the contractor, etc. It generally is easier for an single independent to contract for the de-installation and re-installation without regard to the shipping. In that case, you have a, hopefully, expert and unbiased third opinion as to the condition of the instrument both before and after the actual shipping. In court, that third opinion may well carry the day as definitive.
If the independent has arranged for the shipping, they will be seen as having a biased interest in the overall operation. The lines between the preparation for freight, the delivery of freight and the receiving of freight will be blurred and any court may appropriately spread the blame for a problem between the independent and the freight company. In other words, there will be no way to definitivily identify the source of a problem. In the worst case, a court may not be able to assign blame to any identifiable source.
An elecron microscope is a sensitive instrument, subject to many mechnical and electronic variables. A major move of an instrument like this should involve an objective assestment of the instrumental operation before the move and an objective assesment of the instrumental operation after the move. Any problems can then be identified to the source.
In all honesty, I have yet to see any problem in moving any SEM. However, considering the potential losses involved in moving a recent model SEM, these problems should be kept in mind.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com
-----Original Message----- } From: Dr. Gary Gaugler {gaugler-at-calweb.com} To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
a database containing user manuals for a range of older popular microscopes would be a very good idea, especially since the purchase of a second hand microscope is usualy the only way for an amateur microscopist to get hold of a decent microscope at a (more or less) reasonable price. That's why I have put some Reichert Zetopan manuals on my website.
In the best case scenario this service should be easy accesible and free if at all possible. If that isn't possible a small fee could be asked to cover the costs.
But it isn't simple:
I don't think that the large manufacturers would like to support this idea, as it isn't in their short-term intrests to support users of second-hand equipment, they rather like to sell new gear (one example: I know from a very reliable German source, that Zeiss has, after the "wende", destroyed large stocks of spare parts for older "Eastern-German Zeiss manufactureres"). I think they see it the wrong way: I can't imagine much amateurs who spend the exorbitant prices the large manufactureres ask for their products, at least I wouldn't...
And, unfortunally, as far as I know, the manufacturers are the owners of the copyrights of their manuals, so we're stuck here, that would be the first problem to be solved...
After that: finding someone to coordinate the project, finding the manuals and scan those (can't be much of a problem I suppose), finding a server to host the documents and finding some people to do investigations regarding brands, models, serial numbers... to match manuals and models/versions...
I would like to volunteer for such a project...
Hello Royal microscopical society (England), Microscopy Society of America, German microscopy clubs, Micscape Magazine, manufacturerers...?
Yvan Lindekens.
-----Oorspronkelijk bericht----- Van: donald j marshall {dmrelion-at-world.std.com} Aan: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com} Datum: donderdag 8 april 1999 1:26 Onderwerp: manuals
} I would just like to support and reinforce some of the many comments } recently made about getting copies of older instruction manuals. It would be } a real service to our community if a master compilation of these manuals, } with a suitable index and regular updates, could be put together. I wish } that I had the time and the resources to volunteer for this task but I } don't at the moment. Hope somebody else does. } } Don Marshall } } }
We're a film production company and need to shoot some footage through a stereo microscope -- stereoscopically. Would someone be so kind as to point us to a source for a scope to which *two* videocameras could be attached, one for the right field and one for the left? Please reply to JPWELT-at-aol.com.
Hi Bo, We have for sometime been using LRW for Immuno-staining for plant tissue. We have based our protocol on that of Kathryn A. Vandenbosch and a good reference is in Chapter 5 Immunogold Labelling in Electron Microscopy of Plant Cells eds.J.L.Hall & C.Hawes,Academic Press 1991. Our protocol uses medium grade LRW, it is often benificial after fixation to add some Ruthenium Red to the buffer wash to stain the tissue as later its refractive index will be the same as the resin and specimens are easily lost. AS you specimens are in 70% Alc already dissolve the stain in water and use that to dilute 100% Alc to 70%Alc to stain the tissue. If you have any problems obtaining the reference i will send you our protocol.
I would suggest using a pulse of BrdU. We use it routinely as a marker for cell proliferation. It is a non-radioactive thymidine analog. We use Dako's anti Brdu and DAB as the chromagen. Feel free to call me for specifics.
-- Begin original message --
} For a project on cartilage regeneration in dogs, I would like to be able to } determine the origin of the new cartilage cells. I know that one way would } be a classic pulse-chase experiment with tritiated thymidine to label } dividing cells, but I'm wondering whether there might non-radioactive } methods that could tell me the same thing. I'd prefer not to have to inject } radioactive solutions (even tritium) into these animals. Any ideas? } } Gary Radice 804-289-8107 } Department of Biology 804-289-8233 (FAX) } University of Richmond gradice-at-richmond.edu } Richmond VA 23173 -- End original message --
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress ... But I repeat myself.-Mark Twain**
Just take the Stereo microscope. and have two exact cameras, and two exact camera to microscope eyepiece adapters.
Ed Sharpe
}
Hello -
We're a film production company and need to shoot some footage through a stereo microscope -- stereoscopically. Would someone be so kind as to point us to a source for a scope to which *two* videocameras could be attached, one for the right field and one for the left? Please reply to JPWELT-at-aol.com.
I do not know of any write ups on the web for preparation of thin sections but Allied High Tech (I work for Allied High Tech) has a quick curing epoxy (5-10 min.) called Epoxy Bond 110. We also have extensive experience preparing thin sections, samples for Metallography, SEM and TEM samples. I am located in Raleigh and would like to work with you and help you develop your applications.
We also have a precision polisher, the MultiPrep system. It has a vertical spindle that prevents unwanted faceting. The spindle is calibrated perpendicular to the abrasive and the specimen is calibrated parallel to the abrasive. If a specific angle is required it can be set too. The MultiPrep system also allows you to monitor the amount of material that has been removed from the specimen in real time. The system is ideal for parallel polishing, preparation of thin section, SEM and TEM specimens.
If you would like additional information about the MultiPrep system or any of Allied's products please contact me off-line. I may be reached via email or at the phone number listed below. All of Allied's products are also on our web site. The address is also below.
Regards,
Ed Hirsch
At 11:10 AM 4/8/99 -0400, Roberto Garcia wrote:
} } } }
{excerpt} Hello everyone. I would like to know if there is a quick write-up on the web that explains how to prepare thin sections of rocks and sand for microscopy. I need to know specifically what type of epoxy is used to affix the specimen to the microscope slide. Thanks.
Hello I am looking for a provider of single Crystal cristal viewing screens ( ma= de of YAG), that are used as a pick up screens in image capture system to TEM. any suggestion are welcome thanks =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D Fernando D. Balducci Laboratorio de Microscopia Electr=F3nica Facultad de Ingenier=EDa - Bioingenieria Universidad Nacional de Entre Rios Argentina e-mail: microsc-at-fi.uner.edu.ar tel: 54 43 975100 fax: 54 43 975077
Ingber, Bruce F. wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } ---------- } } From: Earnhart, James P. } } Sent: Monday, April 05, 1999 7:25 AM } } To: Ingber, Bruce F. } } Subject: RE: Mech Pump Discharge Backpressure } } } } Only to say that if the pumps were designed to be able to } } operate in the described manner then the manufacturers would have specs on } } how and what type of ventilation system to install under different } } applications, i.e. extraction type if there is more than 10' of pipe to } } outside, or more than 2 bends totaling more than 90 degrees. Also, if } } discharging the "bad air" outside then EPA standards must be met...such as } } installing "scrubber" systems to insure no oil or certain hydrocarbons are } } discharged into the air and eventually being introduced into the ground } } water. Best thing is to install a factory offered oil recovery exhaust } } filter system such as the one we spoke about on the coating system. And } } to check with OSHA as to the "hazards Imposed" by the Vacuum Pump vapors. } } If they say they are too hazardous for normal laboratory environments then } } we may explore the possibility of routing the exhaust into existing "Fume } } Hoods" in the laboratories. That is assuming they have the necessary } } environmental "cleaning" systems built into them. } } } } Bottom line is I doubt that without a lot of engineering the } } pumps will be able to be operated properly with the exhaust "routed" } } through any type of piping more than a few feet long. Meaning that if you } } hook any lines, more than a few feet long without bends or any that have } } more than 90 degrees of bends, to remove the fumes to any of the pumps, } } they won't work properly without a lot of engineering to eliminate any } } "back pressure" that may be caused. } } } } ---------- } } From: Ingber, Bruce F. } } Sent: Thursday, April 01, 1999 3:43 PM } } To: Earnhart, James P., Electronic Technician } } Subject: FW: Mech Pump Discharge Backpressure } } } } Any ideas? } } } Bruce F. Ingber } Biologist- Electron Microscopy } USDA-ARS, SRRC } 1100 Robert E. Lee Blvd. } New Orleans, LA 70124-4305 } } (504) 286-4270; fax (504) 286-4419 } bingber-at-nola.srrc.usda.gov } } } ---------- } } From: Lehman, } } Ann[SMTP:Ann.Lehman-at-exchange.cc.trincoll.edu] } } Sent: Thursday, April 01, 1999 9:56 AM } } To: Microscopy-at-sparc5.microscopy.com } } Subject: Mech Pump Discharge Backpressure } } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy } } Society of America } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } -----------------------------------------------------------------------. } } } } } } Dear Listers, } } } } My Safety Officer wants all our mechanical pumps } } vented outdoors. The pumps } } are Sargent-Welch, Alcatel, and Edwards and are used } } to back TEMs, film } } desiccators, evaporators, etc. Initially I thought } } this was a good idea, } } having done it simply in past lives by drilling a } } hole in a wall, ganging } } the outlet hoses, and presto - out the bad smoke } } went. } } } } However the B&G engineering consultant got hold of } } the project (yes now it's } } a 'project') and now EVERY pump must get its own } } line up to the roof. This } } means each pump's discharge is directed to a run of } } 1-inch copper pipe with } } three to five 90-degree bends over a total vertical } } length of about 20'. } } (Luckily I am on the top floor.) There is also talk } } about inserting a } } clean-out or filter at each feed-through for dealing } } with accumulated oil. } } } } I'm concerned that the pumps are not designed for } } backpressure on the outlet } } side coming from the 20' column of air plus the } } resistence from the } } 90-degree bends and filter. } } } } Could this setup affect the (1) efficiency or (2) } } overall life of the pumps? } } Am I being overly cautious? } } } } I'd appreciate feedback from the List (including } } manufacturers) re the } } feasiblity of this approach, and the pump specs - I } } haven't been able to } } find anything about discharge 'load' tolerances. } } } } Thanks, you can reply offline and if there is } } sufficient interest I'll } } summarize responses for the List. } } } } Ann Hein Lehman } } EM Facility Mgr } } Trinity College } } Hartford, CT 06106 } } v. 860-297-4289 } } f. 860-297-2538 } } e. ann.lehman-at-exchange.cc.trincoll.edu } } } } } }
Bruce, Unless your lab is at a partial vacuum and you are venting your pump to atmosphere, backpressure is a moot point. The gas flow is miniscule except for initial roughing.
Runs, elbows, diameters, restrictions are all critical on the INTAKE side of the pump, but have no significant effect on the outlet side of the pump.
We used LR White in order to embedd immobilised root protoplasts in alginate beads.
After dehydration try 96% EtOH 2x10 min, 100% EtOH 2x10 min and infiltrate with LR White, 3x1 hr at room temperature, transfer to gelatine capsules. Polymerization takes place at 60 C, 48 hr.
Gary.
On Thu, 8 Apr 1999, Bo Johansen wrote:
} Hi, } } can somebody help me with a protocol for embedding plant material in LR } white. The material is fixed in PFA and kept in 70% EtOH at -20 C. } } Bo } } -- } Dr. Bo Johansen } Associate Professor } Botanical Institute } University of Copenhagen } Gothersgade 140 } DK-1123 Copenhagen K } Denmark } } Tlf: + 45 35 32 21 57 } email: boj-at-bot.ku.dk } http://www.bot.ku.dk/staff/boj.htm
Dear Sir/Madame, Could you please tell me the going rate of using a TEM (dollars/hour) with and without a technician? Thank you in advance, Toni Milroy
I have two unrelated projects with a common element - I need to be able to get particles dispersed nicely across an SEM stub (and one of them on a TEM grid, as well). One is biological, the other material.
Tiny particles tend to clump as the solution they are in dries. In one case a client is trying to look at fungal spores, and in another someone is trying to look at tiny metal needles. I would love to hear all your expert suggestions, since this comes up often!
Snow in San Jose? It's in the low 80s F, sunny, a bit windy here on Oahu.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Here is a possible cheap digital solution for you. I think that you will have to go through the eyepieces to get stereo. The following message was from a rep about a digital camera that could be inserted in the eyepiece. Two might give you what you want. Call and find out. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
--------message follows-----
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---------- } From: "ICEMANCINE-at-aol.com"-at-Sparc5.Microscopy.Com To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Hello -
We're a film production company and need to shoot some footage through a stereo microscope -- stereoscopically. Would someone be so kind as to point us to a source for a scope to which *two* videocameras could be attached, one for the right field and one for the left? Please reply to JPWELT-at-aol.com.
Dear Tina, } } Tiny particles tend to clump as the solution they are in dries.
If the solution wets the particles, that will certainly be true; however, if not, and the particles are attracted to the grid/formvar/ carbon, etc., then the solution should not drag them together. The solution to the solution is to find one which has the properties needed; for the metal particles, try to make the grid hydrophyllic (assuming the needles are wet by water) and use a nonpolar solvent such as petro- leum ether, and if the fungal spores have a static charge, the same may work for them. If the fungal spores are hydrophobic, try a bare formvar coat and ethanol (C-coat afterwards). You may have to fiddle with the grid-solvent combinations a lot, so good luck.
} In one } case a client is trying to look at fungal spores, and in another someone } is trying to look at tiny metal needles. I would love to hear all your } expert suggestions, since this comes up often! } } Snow in San Jose? It's in the low 80s F, sunny, a bit windy here on Oahu. } We are actually having springlike weather in Albany (~60 F, no snow) in spite of the dire predictions based on La Nina. Yours, Bill
Fernando D. Balducci wrote: ============================================== I am looking for a provider of single Crystal cristal viewing screens ( made of YAG), that are used as a pick up screens in image capture system to TEM. any suggestion are welcome =================================================== SPI Supplies has offered YAG screens for some number of years, in different dimensions and different thicknesses. Quite a bit of technical and other information about these screens is available on our website URL http://www.2spi.com/catalog/scintill/spi-tem-yag.html
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
Dear Robert, } } Two related items cause me to respond to the list. } 1. How do we know what we see through the microscope is real? } 2. Alan Sokal and the "Science Wars." } } There is a group of social scientists who are known as social } constructionists. Of this group there are those known as } deconstructionists. And of this group there are those who are engaged in } the "Science Wars". These individuals more or less advocate that what } scientists do is a construction of their mind and not necessarily an } expression of nature. Some continue to argue that the actions or } constructions of scientists is done in part to get money to play around } in the lab. Some of these people ask that federal funding be curtailed } for these constructions of people called scientists. In some way the } arguments remind one of vegans attacking medical research. } They are partially correct--the half-truth is that everything we do is filtered through our individual perceptions--but they leave out the part of science which insists that all discoveries must be reproducible by an independent observer. This limits science in a way that art, for example, is not, but it gives the scientific method more power and generality than the other "theories" which the deconstructionists compare it to. It is clear to any experimental scientist that scientific observations are, at best (which is how *we* do them), approximations to reality, and that theories are also approximations, since only limited evidence is available to test them. It is true that without funding no scientist can function for very long, so it follows that part of the motivation for our activities is to obtain funding. However, each of us can say with confidence that science is a unified body of knowledge, even though much of that knowledge eludes us, and that the entirety of scien- tific knowledge has benefitted mankind far more than the cost to produce it (can the deconstructionists make the same claim?).
} Alan Sokal wrote an article call Transgressing the Boundaries:... and it } was accepted and published in the journal Social Text. This journal with } a circulation of about 800 is a leading journal for social } constructionists and a place where debates about the science wars have } been taking place. Sokal, an avowed leftist physicist, wrote } Transgressing as a hoax and immediately proclaimed so when the Social } Text article was published. His purpose was to show that the Science } Wars advocates were on thin intellectual grounds. You may wish to read } several recent articles of Academe (an AAUP publication) that tries to } put this all into focus. } } So back to the microscope. Those of us who do microscopy know that much } of what we look at is a construction. The tissue is dead, chemically } altered, stained, dehydrated, infiltrated, and sectioned into to little } pieces... AT BEST. Clearly we are constructing what we hope is a correct } interpretation of nature. Akin to walking through a graveyard and trying } to guess what really happened in the living lives of the people under the } tombstones.
Archeologists, in fact do essentially this. There is nothing in- herently wrong about making inferrences about reality based only on a severely modified part of that reality. As long as we realize the con- sequences of the modifications and do not over-interpret our results, we will be on reasonable safe ground. However phenomena such as the micro- trabecular network, which was shown to be an artefact, should serve to remind us of the pitfalls waiting for the unwary.
} We also know in the best sense of Popper that we are self } doubting and trying to better (disprove) much of what was published } before. These social constructionists do not seem to understand any of } this. Just like I don't understand why people watch soap operas on } daytime television. } Can one get funding by watching soap operas?
} So the question put forth in the original post is a very important one. } How do we tell a public what we see through the microscope is real?
We don't. We tell them that we see a more-or-less reasonable representation of reality which we continually strive to understand and improve, and we tell them that there are limitations to how close any particular method can come to reality (this is why some of us do cryo- EM on unstained material, which also has its limitations).
} It } is a kind of "Daddy, why is the sky blue?" question. As microscopists we } have a responsibility to address the question and help the public } understand what we see is "real" and represents nature.
The "" around real are well deserved. Only by being aware of the representational and limited aspects of science will we really help the public understand what we do.
} And yes it is a } construction of sorts but that is what science is all about: Humans' } feeble attempt to reconstruct the beauty of nature... but not in the } sense of the deconstructionists. } Agreed.
} I recognize this is an unusual thread for this microscopy list, but this } is an important issue because it can dramatically affect the funding that } many of us share. } Not to mention the little detail that without serious considera- tion of the nature of the scientific process, we will not get far in our search for the nature of reality. Yours, Bill Tivol
I was searching the Internet to find some information about STM (Scanning Tunneling Microscopy). This is about to write down a technical paper covering the physic apsects involved in STM.
Anyone could send me good links? Any link / good information in spanish?
{ {Tiny particles tend to clump as the solution they are in dries.} }
I find that a drop of poly-L-lysine in the suspension works wonders for most particle types and allows for a quite even distribution on filmed TEM grids. I have used it mostly on non-biological samples.
I use the standard 0.1% solution offered by EM supply companies. I add one drop of that (from a pasteur pipette) to 1 ml of suspension. It spreads well on all TEM grid films. Would be worth trying on SEM mount surfaces.
Am interested in hearing how it works.
Ted Dunn Maui, Hawaii
The surf is up and it is so beautiful here just now - Spring skies and brisk trade winds.
We are trying to prepare SrTiO3 (001) single crystal TEM samples by chemical means. Does anyone know a good solution to use?
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
Generally the customer wants one source for responsibility. The questions about objectivity by using a third party only complicates the matter. When I was a customer using and maintaining SEMs and other equipment, we subcontracted different portions of the job to the lowest quailified bidder. Thereafter, no one person assummed responsibility for the entire job and when things went wrong all the subcontractors pointed "their collective fingers" at each other and ultimately at us (the customer) for not co-ordinating things properly.
When we move an SEM we use subcontractors that we have experience with for crating and moving. We don't have a vested interest in using these subcontractors (commissions or kickbacks) other than the fact that we have used them before and trust them.
I also take pictures of the SEM and have the movers watch me take these pictures. I FEDEX the pictures to the customer so their receiving department has a record of the condition of the SEM when it arrives.
In the eighteen years we have service SEMs and moved them I have had only one problem: moving a JEOL IC 845. It was stored in a warehouse for one week before shipping. Unfortunately, in transporting the SEM around the warehouse, the movers dropped the column. Questions arose about the SEM being packed properly. The insurance company (Yes I always insure the shipment) tried to "relieve themselves of responsibility". The insurance company said that the shipment was not secured properly as Scanservice did not have the experience to pack the equipment. Of course the insurance company had no idea what was required to pack an SEM, much less what an SEM is. I made one phone call to the moving company (United Van Lines) and assured them that if they wanted our continued business they would re-imburse us for the SEM. Within one day, upper management at United called and assured me that they would pay any and all damages no matter what the insurance company stated. Within one week, we were re-imbursed for our services, the customer was fully re-imbursed and all are happy.
I continue to do business with United even though they are at times slightly higher.
I shutter to think what would happen if we had several other parties involved in dis-assembly crating, moving, uncrating, and re-assembly.
The SEM is a comlicated piece of equipment but after 25 years experience it complexity seems much more trivial. Any system is the sum of it's components. When I first repaired SEMs, they were considered very "Hi tech". The "Hi tech" machines of today will soon be mundane. Look at computers. We are not reluctant to assume total responsibility for the SEM move.
This is not to say that you are wrong. May independents may or may not be willing to accept full responsibility for the move. We will as long as the customer understands that he needs to use sub-contractors that we recommend.
Earl Weltmer Scanservice Corporation
Allen R. Sampson wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Actually, a rather complicated subject. In regards to your overall } question, anyone is capable of arranging the shipping. That is a simple } matter. Many independents, however, may be reluctant to accept the } responsibility. If something goes wrong, you might end up with multiple } lawsuits - you sue the contractor, who sues the shipper, who counter-sues } the contractor, etc. It generally is easier for an single independent to } contract for the de-installation and re-installation without regard to the } shipping. In that case, you have a, hopefully, expert and unbiased third } opinion as to the condition of the instrument both before and after the } actual shipping. In court, that third opinion may well carry the day as } definitive. } } If the independent has arranged for the shipping, they will be seen as } having a biased interest in the overall operation. The lines between the } preparation for freight, the delivery of freight and the receiving of } freight will be blurred and any court may appropriately spread the blame for } a problem between the independent and the freight company. In other words, } there will be no way to definitivily identify the source of a problem. In } the worst case, a court may not be able to assign blame to any identifiable } source. } } An elecron microscope is a sensitive instrument, subject to many mechnical } and electronic variables. A major move of an instrument like this should } involve an objective assestment of the instrumental operation before the } move and an objective assesment of the instrumental operation after the } move. Any problems can then be identified to the source. } } In all honesty, I have yet to see any problem in moving any SEM. However, } considering the potential losses involved in moving a recent model SEM, } these problems should be kept in mind. } } Allen R. Sampson } Advanced Research Systems } 317 North 4th. Street } St. Charles, IL 60174 } PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com } } -----Original Message----- } } From: Dr. Gary Gaugler {gaugler-at-calweb.com} } To: MSA listserver {Microscopy-at-sparc5.microscopy.com} } Date: Wednesday, April 07, 1999 9:31 AM } Subject: SEM moving } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I would appreciate hearing from and about independent companies } } or individuals who take down, move and set up SEMs. Specifically, } } I would like to receive quotes for moving an Amray 1830 from TX } } to Sacramento CA. This would involve disconnecting the system, } } locking down the turbo pump, crating, moving, and re-install at my } } location. } } } } I have a quote from Amray but they do not handle moving. I am } } wondering if this is standard practice or if there are reputable folks } } who can handle the whole job from start to finish via one contact. } } I would anticipate that someone who knows about this specific SEM } } model would be preferred. } } } } Anticipated timeframe for start of project is in about 1-2 months } } from now. } } } } gary g. } } } } my fax is 916.791.8186 } } telco is 916.791.8191 } } } } } }
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear Robert, } } Two related items cause me to respond to the list. } 1. How do we know what we see through the microscope is real? } 2. Alan Sokal and the "Science Wars." } } There is a group of social scientists who are known as social } constructionists. Of this group there are those known as } deconstructionists. And of this group there are those who are engaged in } the "Science Wars". These individuals more or less advocate that what } scientists do is a construction of their mind and not necessarily an } expression of nature. Some continue to argue that the actions or } constructions of scientists is done in part to get money to play around } in the lab. Some of these people ask that federal funding be curtailed } for these constructions of people called scientists. In some way the } arguments remind one of vegans attacking medical research. } They are partially correct--the half-truth is that everything we do is filtered through our individual perceptions--but they leave out the part of science which insists that all discoveries must be reproducible by an independent observer. This limits science in a way that art, for example, is not, but it gives the scientific method more power and generality than the other "theories" which the deconstructionists compare it to. It is clear to any experimental scientist that scientific observations are, at best (which is how *we* do them), approximations to reality, and that theories are also approximations, since only limited evidence is available to test them. It is true that without funding no scientist can function for very long, so it follows that part of the motivation for our activities is to obtain funding. However, each of us can say with confidence that science is a unified body of knowledge, even though much of that knowledge eludes us, and that the entirety of scien- tific knowledge has benefitted mankind far more than the cost to produce it (can the deconstructionists make the same claim?).
} Alan Sokal wrote an article call Transgressing the Boundaries:... and it } was accepted and published in the journal Social Text. This journal with } a circulation of about 800 is a leading journal for social } constructionists and a place where debates about the science wars have } been taking place. Sokal, an avowed leftist physicist, wrote } Transgressing as a hoax and immediately proclaimed so when the Social } Text article was published. His purpose was to show that the Science } Wars advocates were on thin intellectual grounds. You may wish to read } several recent articles of Academe (an AAUP publication) that tries to } put this all into focus. } } So back to the microscope. Those of us who do microscopy know that much } of what we look at is a construction. The tissue is dead, chemically } altered, stained, dehydrated, infiltrated, and sectioned into to little } pieces... AT BEST. Clearly we are constructing what we hope is a correct } interpretation of nature. Akin to walking through a graveyard and trying } to guess what really happened in the living lives of the people under the } tombstones.
Archeologists, in fact do essentially this. There is nothing in- herently wrong about making inferrences about reality based only on a severely modified part of that reality. As long as we realize the con- sequences of the modifications and do not over-interpret our results, we will be on reasonable safe ground. However phenomena such as the micro- trabecular network, which was shown to be an artefact, should serve to remind us of the pitfalls waiting for the unwary.
} We also know in the best sense of Popper that we are self } doubting and trying to better (disprove) much of what was published } before. These social constructionists do not seem to understand any of } this. Just like I don't understand why people watch soap operas on } daytime television. } Can one get funding by watching soap operas?
} So the question put forth in the original post is a very important one. } How do we tell a public what we see through the microscope is real?
We don't. We tell them that we see a more-or-less reasonable representation of reality which we continually strive to understand and improve, and we tell them that there are limitations to how close any particular method can come to reality (this is why some of us do cryo- EM on unstained material, which also has its limitations).
} It } is a kind of "Daddy, why is the sky blue?" question. As microscopists we } have a responsibility to address the question and help the public } understand what we see is "real" and represents nature.
The "" around real are well deserved. Only by being aware of the representational and limited aspects of science will we really help the public understand what we do.
} And yes it is a } construction of sorts but that is what science is all about: Humans' } feeble attempt to reconstruct the beauty of nature... but not in the } sense of the deconstructionists. } Agreed.
} I recognize this is an unusual thread for this microscopy list, but this } is an important issue because it can dramatically affect the funding that } many of us share. } Not to mention the little detail that without serious considera- tion of the nature of the scientific process, we will not get far in our search for the nature of reality. Yours, Bill Tivol
Funny how we were just talking about vacuum pump oil vapours and how to deal with them... Last Tuesday I had a guy in the lab to change the locks (long, unrelated story), including the lock on the door right behind our ESEM. Our vacuum lines pass through a cut-out on this door to the pumps located in a big warehouse-like area. We have the usual little filters on the pumps, but as we all know, you can usually smell a little something in the air anywhere near them. While changing the lock, the guy was standing very close to the pumps for perhaps 10 minutes or so, and said he could smell something "oily". Well, I just found out that later that day, he became quite ill, with apparently a toxic reaction to a substance or substances unknown, and was hospitalized for a few hours, though he later, apparently, recovered completely. We're not absolutely sure that breathing our oil vapours for a few minutes is actually what caused the reaction, but it appears to be the only possible toxin the man was in contact with that day ( or at least that's the story). It's possible that the gentleman just had an unusual sensitivity to the vapours; I know at times I've had lots more exposure than he did, and have personally never gotten so much as a headache. One thing bothers me a bit, though; we use Alcatel 102, an oil specially made for ESEM's, with their increased water throughput, and I wonder if this stuff might be a bit less user-friendly than the more common types. Has anyone else out there ever seen, or heard of, a toxic reaction to this or any other pump oil? (I have a funny feeling that I haven't heard the end of this incident yet...)
F.C. Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
What brands and types of razor blades are used as a disposable microtome knive (paraffin, manual rotary microtome)? What are their limitations regarding section thickness for non-problematic animal and plant tissues?
Alvaro: The links from our site include a section with 11 STM=20 sites. No doubt those sites in turn would have links to=20 most significant STM sites available. Cheers Jim Darley
ProSciTech Microscopy=20 PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Saturday, April 10, 1999 10:19 AM, =C1lvaro Hern=E1ndez=20 Tortosa [SMTP:aht-at-mx3.redestb.es] wrote: } } Hi everybody! } } I was searching the Internet to find some information } about STM } (Scanning Tunneling Microscopy). This is about to write } down a technical } paper covering the physic apsects involved in STM. } } Anyone could send me good links? Any link / good } information in spanish? } } Thank you, } } Alvaro. }
In a message dated 4/10/99 6:43:54 PM EST, gbza40-at-udcf.gla.ac.uk writes:
{ { Not to mention the little detail that without serious consideration of the nature of the scientific process, we will not get far in our search for the nature of reality. Yours, Bill Tivol } }
I thoroughly enjoyed your posting. Thanks.
The word REALITYcan be interchanged with TRUTH. I don't believe that in a wider sense the scientific process necessarily has anything to do with one's ability to know Truth.
This is my first posting to this listserver. I am in position to purchase a Jeol 840A sem in good condition equipped with a Kevex 8000 EDS detector and need to know its approximate value on the used market. Responses can be made directly to me or via the listserver.
The ion pump on our JEOL 2010 (LaB6) has just about died and we were wondering what sort of lifetimes people were getting for pumps on similar (JEOL 2000 series) machines. Our samples, though biological are not changed often and even so, with the configuration of the machine ie differential pumping etc. we would not expect the ion pumps to have to work terribly hard. We have had the microscope for around 5 years and would like to know if that is about normal for these pumps.
We were also curious as to what people did when the pumps finally fell over. As far as we know there are 3 options - replace with new pumps, replace with reconditioned pumps or replace the filaments in the existing pumps.
What have people found to be the most "cost effective" solution?
Thanks for any help with this.
Colin Veitch
Instrumentation Scientist Fibre Structure & Function Group CSIRO Wool Technology PO Box 21 BELMONT Vic 3216 Australia
Tel: +61 (0)3 5246 4000 Fax: +61 (0)3 5246 4811
The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Wool Technology on +61 3 5246 4000.
I was shown a very simple technique for dispersing dry particles on a SEM stub by Dave Gittens in PGT(UK).
Take a sheet of paper & staple it in a funnel shape. Place a small amount of the powder in the funnel & hold the stub ( with adhesive ) at the end. Use compressed air/N2 to propel the powder out of the funnel onto the stub. It may take a bit of trial & error to get the level of dispersion right, but it should give a very even dispersion without many particles touching. Obviously this should be carried out in a fume hood for safety.
Colin
Colin Reid, Electron Microscope Unit, Trinity College Dublin, Dublin 2, Ireland. Tel: 353-1-6081820 Fax: 353-1-6770438 email: creid-at-tcd.ie -----Original Message----- } From: Tina Carvalho {tina-at-pbrc.hawaii.edu} To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com}
The lifetime of the ion pump of our Jeol 2000FX was 5 years. Officially, JEOL says that the lifetime of a recondioned pump is three years and a new pump five years. Since the microscope is 10 years old, it was the second ion pump to be changed. We choose to replace the pump by a reconditioned pump. This solution save about 50% of the price of a new pump. In fact, this solution was prefered because the reconditioning consists in changing the storing elements and the result is a new pump. The only risk resides in the possibility of getting a very old pump and in this case some soldering can be defective, but it is very rare. Anyway, the pump is always tested before installation and the risk is quasi null.
Eric LEROY Dr. Laboratoire de Chimie Metallurgique des Terres Rares UPR 209 - CNRS Groupe des Laboratoires de Thiais 2-8, rue Henri Dunant 94320 THIAIS cedex
Hi Colin, Tina, Using a compressed air stream, or any moving air for that matter, is a good way to classify particles. I'm not saying it cannot be done, just that I would be very uneasy about a proper sampling. Dispersing particles is not an easy process, although it can seem that way. It would be nice if there were some simple solution, but unfortunately there isn't. We have found the best results by dispersing in an organic solvent with a dissolved polymer carrier and casting films on either water or cleaved mica. Even this technique has it's drawbacks and limitations. Particle material, density, shape, size, size distribution, surface energy and tribo properties and all have affects on dispersability. In other words the best technique for your particles must be determined by you by trials and more trials. Good luck, Russ Gillmeister, Xerox
-----Original Message----- } From: Colin Reid [mailto:creid-at-tcd.ie] Sent: Monday, April 12, 1999 1:27 AM To: MSA.Microscopy.Com
Hi Tina,
I was shown a very simple technique for dispersing dry particles on a SEM stub by Dave Gittens in PGT(UK).
Take a sheet of paper & staple it in a funnel shape. Place a small amount of the powder in the funnel & hold the stub ( with adhesive ) at the end. Use compressed air/N2 to propel the powder out of the funnel onto the stub. It may take a bit of trial & error to get the level of dispersion right, but it should give a very even dispersion without many particles touching. Obviously this should be carried out in a fume hood for safety.
Colin
Colin Reid, Electron Microscope Unit, Trinity College Dublin, Dublin 2, Ireland. Tel: 353-1-6081820 Fax: 353-1-6770438 email: creid-at-tcd.ie -----Original Message----- } From: Tina Carvalho {tina-at-pbrc.hawaii.edu} To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com}
In my former position as the TEM Technical Specialist at NYS College of Ceramics for 6 years, I was the lucky one who had to make the same decision about the sputter ion pump (SIP) on our JEOL 2000FX. The microscope was installed in early 1987 and the SIP died in May 1994. I elected to have it reconditioned which resulted in no problems other than the amount of time it took since we did not have a service contract on the TEM. The reconditioned SIP was working up to the time I left that position (Aug. 1998) and is still working as far as I am aware. Since ion pumps are classified as solid entrainment type pumps, their lifetime depends on their pumping history, i.e. operating pressure. The SIP on that particular JEOL 2000FX was typically operating in the mid to high 10-7 Torr range in standby mode, which resulted in a lifetime of 7.5 years. The same operating condition has been maintained for the reconditioned SIP and it is currently coming up on 5 years this summer. Also, the cost for reconditioning was ~$1020 + shipping (US-1994 dollars). Good luck!
David
* * * * * * * * * * * * * * * David T. Hoelzer, Ph.D. Metals and Ceramics Division Oak Ridge National Laboratory Bldg. 5500, Mail Stop 6376 P. O. Box 2008 Oak Ridge, Tennessee 37830 (423) 574-5096 {Work} (423) 574-0641 {Fax}
Colin asks ... } } } Hi All, } } The ion pump on our JEOL 2010 (LaB6) has just about died and we were } wondering what sort of lifetimes people were getting for } pumps on similar (JEOL 2000 series) machines. ...
5 years should be considered a better than average lifetime. We had a problem replacing the ion pump on our 6300 SEM, because it was a JEOL pump, which has since stopped supporting (... at least they didn't have one on their shelf when we asked, and instead wanted to re-furbish the existing pump elements ...). Since the JEOL's IP HV connector was a non-standard type, we then decided to go with the same capacity and an established 3rd party and a standard connector. It could be that your IP has a standard connector, and that all you have to do is replace the elements. It would be also just as cost effective to have JEOL or a 3rd party service refurbish your elements. Most cost effective would be to extend the pump's lifetime by baking it out, but not all elements can be baked effeciently or at all. And, yet another option, is to try and find a replacement element which can accommodate an "in situ" bake-out heater, altho more probably, this may mean replacing the entire pump.
I have more info around here somewhere, but can't easily find it ... but I did find the 3rd party refurbishing and replacment services via searching the wwweb with "metacrawler.com".
... hope this helps :o)
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
Hi Colin, I'm replying to the List because my system thinks your address is invalid (due to two "-at-" symbols?).
I inherited a TEM with an IGP that was less than 5yrs old. It was used in a rapid-turnaround pathology lab. Both the samples and the film were cycled very quickly through the system. (In fact, this scope would frequently crash because the film was used up so quickly, the auto-sequence for pumping the column was interrupted.) No one used the cold trap. The IGP became exhausted and had to be replaced. Luckily we had covered the IGP as a rider on a service contract.
The reason for its early demise was attributed partly to the biological specimens that essentially put a lot of water into the vacuum system but mostly to the fact that the scope and film desiccator were backfilled with impure (wet) nitrogen. (Room air would have been even worse.) The nitrogen we used was run-of-the-mill 'standard' nitrogen, and the service contract provider wanted to nullify the IGP rider coverage because of it. Lucky for us, since the rider did not specify what TYPE of nitrogen to use, we were covered. Once the pump was replaced and we switched to UHP (ultra-high purity) nitrogen for backfilling, the system performed fine.
I was not there long enough to complete another 5yr study; however, I now have another IGP system and I do specify UHP nitrogen. I also use the cold trap. Someone else may add to this, but I was told this delivers any specimen-derived water vapor to the vacuum system in a slow, controlled fashion as the trap heats up (rather than all at once).
Hope this helps.
Ann Hein Lehman Trinity College Hartford CT
-----Original Message----- } From: "colin.veitch-at-dwt.csiro.au"-at-Sparc5.Microscopy.Com [mailto:"colin.veitch-at-dwt.csiro.au"-at-Sparc5.Microscopy.Com] Sent: Monday, April 12, 1999 1:02 AM To: microscopy-at-Sparc5.Microscopy.Com
Hi All,
The ion pump on our JEOL 2010 (LaB6) has just about died and we were wondering what sort of lifetimes people were getting for pumps on similar (JEOL 2000 series) machines. Our samples, though biological are not changed often and even so, with the configuration of the machine ie differential pumping etc. we would not expect the ion pumps to have to work terribly hard. We have had the microscope for around 5 years and would like to know if that is about normal for these pumps.
We were also curious as to what people did when the pumps finally fell over. As far as we know there are 3 options - replace with new pumps, replace with reconditioned pumps or replace the filaments in the existing pumps.
What have people found to be the most "cost effective" solution?
Thanks for any help with this.
Colin Veitch
Instrumentation Scientist Fibre Structure & Function Group CSIRO Wool Technology PO Box 21 BELMONT Vic 3216 Australia
Tel: +61 (0)3 5246 4000 Fax: +61 (0)3 5246 4811
The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Wool Technology on +61 3 5246 4000.
I need to pump this group for information (sorry, it slipped out).
The recent discussion of ion pump lifetimes has generated some talk here about the lifetime of turbo pumps. Does anyone have figures for turbos? For example, say one has a TP with magnetic bearings and runs the pump continuously, at what point would one expect some sort of problem due to normal wear and tear? I assume that metal fatigue would be a factor to consider here. Thanks.
While the following list is by no means complete, the following come to mind:
Leica Olympus Triangle Biomedical Accuedge
Ther are several more out there. All of them have their advocates in the histology community.
All these blades will work fine with paraffin processed tissue.
-- Begin original message --
} } Hi all, } } What brands and types of razor blades are used as a disposable microtome } knive (paraffin, manual rotary microtome)? What are their limitations } regarding section thickness for non-problematic animal and plant tissues? } } Thanks in advance for any input! } } Yvan Lindekens.
-- End original message --
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress ... But I repeat myself.-Mark Twain**
there is a short communication in "Powder Technology" from 1971 that describes a eutectic camphor-napthalene mixture that can be used to deagglomerate small particles for observation as individuals. i have used this technique with some success in the past. what it amounts to is dispersing the particles by "kneading" them into the eutectic mixture and then subliming the mixture away to leave only the particles. this avoids the pulling effect of a drying liquid dispersant and helps keep the particles apart.
hope this helps.
ref: Powder Technology, 5 (1971/72) p.377-9.
b-
**************************************************************** Brian McIntyre Electron Microscopy Lab Institute of Optics University of Rochester Rochester, NY 14627
716-275-3058 716-244-4936(fax)
"You may get to the top of the ladder of success only to find its been leaning against the wrong wall" A. Raime
I am experimenting with phosphorous doped silicon. The goal is to determine dopant concentrations in the doped regions between the source/gate and gate/drain. I believe that with the correct recipe, the doped silicon will etch at a faster rate than the undoped regions, revealing thickness fringes when view in the TEM in the WBDF configuration. Thus far I have not obtained the desired results, ie, the etchants seem to attack all areas equally. Do you know of a wet chemical etch recipe which would achieve this result?
i've got a perfectly good noran light element detector that i'd like to keep and plug into a new low-cost rear end electronics and computer system. i've spoken with some vendors (that shall remain nameless) that cannot interface easily to the detector. are there any vendors that can do this and provide qual/quant, digital imaging, and stage control for a reasonable price?
(this may not be of general interest so 'reply' may be most appropriate)
thx b-
**************************************************************** Brian McIntyre Electron Microscopy Lab Institute of Optics University of Rochester Rochester, NY 14627
716-275-3058 716-244-4936(fax)
"You may get to the top of the ladder of success only to find its been leaning against the wrong wall" A. Raime
If the wick is regularly replaced and the operating conditions are clean, the TP should last many years. I have a Balzers 240 that is 11 years old and still works perfectly. It uses a TP120 controller.
JEOL typically sells a JEOL 840 for about $40,000.00 installed with a one year warranty. This is without the EDS. On the used market the 840's have been selling for about $35,000.00 to $45,000.00 with an EDS but not the EDS is not guaranteed working. In other words the EDS and related hardware is given gratis. About half the time I have found them to be in working order as the detectors have been left at room temperature for an unspecified time. I would be heisitant to purchase a Kevex 8000 series as reliability has been an issue with this series because of the wirewrap mother boards. Check with Kevex (now Noran) for the details.
Hope this helps.
Earl Weltmer
Cochran wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi All, } } This is my first posting to this listserver. I am in position to } purchase a Jeol 840A sem in good condition equipped with a Kevex 8000 } EDS detector and need to know its approximate value on the used market. } Responses can be made directly to me or via the listserver. } } Thanks a lot for your time and assistance. } } Ray Cochran
In a message dated 4/9/99 6:14:32 PM US Mountain Standard Time, walck-at-ppg.com writes:
} microscopy-at-sparc5.microscopy.com
We have a most ancient AO microtome with cryostat and a dull ugly blade in a wood box. It would be neat if we could find one of the holders that held the razor blades ... any floating out there??! Ed Sharpe
Turbo pump life time is like asking how long is a piece of string. We = have many pumps on various systems that go for a very long time ( 8 to = 10 years) however we have also had those that go in one year for no = apparent reason.( plus one month just so that they are out of warranty )
However most pumps, from whichever manufacturer, seem to last pretty = long and are very reliable if treated with respect. One of the main = killers of turbos seems to be oxidation and fatigue of the metal = components. Some of the EM's, when switched off, vent the backing line = and thus the turbo. This means that the turbo parts, which are designed = to be under vacuum all the time, oxidise quicker than originally = planned.=20 So my advise is to ensure that the turbo is always on or at least under = vacuum. If you need to leave the system "off" over holiday periods, it = is better to simply unplug the turbo controller and leave the rotary = pump running. This will keep the system under vacuum without the danger = of having a power failure or such like, kill the turbo whilst no one is = around. Servicing the turbo oil wick is also recommended on a regular basis and = our experience shows that once a year is sufficient.=20 But believe me, I would much rather have a turbo system to any diff. = pump or Ion pump system.=20
Cheers Luc Harmsen Anaspec, South Africa Technical support on microscopy. Tel + 27 (0) 11 476 3455 Fax + 27 (0) 11 476 7290 anaspec-at-icon.co.za
-----Original Message----- } From: "JBozzola-at-aol.com"-at-sparc5.microscopy.com = [SMTP:"JBozzola-at-aol.com"-at-sparc5.microscopy.com] Sent: Monday, April 12, 1999 7:03 PM To: Microscopy-at-sparc5.microscopy.com
I need to pump this group for information (sorry, it slipped out).=20
The recent discussion of ion pump lifetimes has generated some talk here =
about the lifetime of turbo pumps. Does anyone have figures for turbos? = For=20 example, say one has a TP with magnetic bearings and runs the pump=20 continuously, at what point would one expect some sort of problem due to =
normal wear and tear? I assume that metal fatigue would be a factor to=20 consider here. Thanks.
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On Mon, 12 Apr 1999 JBozzola-at-aol.com-at-Sparc5.Microscopy.Com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I need to pump this group for information (sorry, it slipped out). } } The recent discussion of ion pump lifetimes has generated some talk here } about the lifetime of turbo pumps. Does anyone have figures for turbos? For } example, say one has a TP with magnetic bearings and runs the pump } continuously, at what point would one expect some sort of problem due to } normal wear and tear? I assume that metal fatigue would be a factor to } consider here.
John,
I have run a Seiko-Seiki Maglev on our JEOL4000 without any problems for 8 or 9 years. It does not give any noticable vibration or magnetic field problem (we resolve 0.25nm) although I do use a Balzers vibration isolator as I was pretty paranoid when I first fitted it in place of the SIP. We have had a few (2 or 3) severe vacuum crashes when it suddenly pumped air and hit the safety bearings but it has survived OK. The reason we replaced the SIP is that the microscope has been modified to include an apertured gas reaction cell so the pump often sees a pretty poor vacuum (10-5 mbar) of H, He, N, O, CO, Ar and various hydrocarbons for several hours at a time. However, the STP can still pull 10-7 mbar on the column given time to clear gasses out of the column properly.
Unfortunately, I have no financial interest in any of the companies mentioned.
Ron
=========================================================================== Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk Department of Materials, phone +44 (0) 1865 273701 University of Oxford, fax +44 (0) 1865 283333 Parks Road. Oxford. OX1 3PH. UK. ============================================================================
Robert, it is a far from easy task to get 'real' numbers out of this. The etch you should use is (roughly) 0.5% HF acid (usual conc., i.e. 48%) in Nitric acid (again, 'usual' conc. - 50%???). At room temperature this will give you the desired effect in a few seconds on a prepared TEM specimen. Au or diamond grids will stop Cu being dissolved from the grid and being re-deposited on your sample. UV light is supposed to be needed for one polarity of dopant, but I can't remember which. I did see one paper a couple of years ago that said that the thickness of the TEM specimen had an effect, so they etched just one side - before doing the second polish to make a thin specimen, and ion milling from the unetched side (I can probably dig this reference out if you like). Ron Anderson from IBM told me they do it at about -100 degrees C in a dark tank for a few minutes; the solution should be a kind of 'slush' like the iced drinks you can get. He said this slowed down the etch rate and ! gave better uniformity and more re In my opinion the experimental variability in this method is likely to be much worse than the FE-SEM method, where you get doping contrast due to work function changes. I'm also not sue whether the TEM method is primarily sensitive to the active or inactive dopant concentration. In either case you will need a planar sample for SIMS to give you a calibration curve.
Cheers,
Richard Beanland
} --------------------------------------------------------------------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } --------------------------------------------------------------------. } } } I am experimenting with phosphorous doped silicon. The goal is to determine } dopant } concentrations in the doped regions between the source/gate and gate/drain. } I } believe that with the correct recipe, the doped silicon will etch at a faster } rate than the undoped regions, revealing thickness fringes when view in the } TEM in the WBDF } configuration. Thus far I have not obtained the desired results, i.e., the } etchants seem to attack all areas equally. Do you know of a wet chemical } etch recipe which would achieve this result? } } Thank you, } } Robert Pomrenke } bpom-at-aol.com
Try Conneaut Lake Scientific in Conneaut Lake, Pa. for what you are looking for.
Just to let you and others know that Mike Gemble the owner/operator has a warehouse full of used microscopes and microscope related items such as preparation equipment and accessories.... He can be reached at myroscope-at-aol.com or his phone is 814-382-1604... If anyone is interested and passing through Norhtwest Pennsylvania, it would be a good place to stop and browse if you are into this type of equipment... Also his web site is www.conneautlakesci.com Check it out!
Good Luck!
C YA C. Passione -----Original Message----- } From: "COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com {"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com} To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}
I am looking for sources to obtain a quality "student" grade microscope for my son, who is 10 years old. Can you or the MSA offer an recommendations or guidance?
I operate and service our Philips CM12. The +15 volt power supply in the remote racks has failed, mainly in the area of the 2 MJH-16010 transistors.
The designation for this supply is PE 1130/00 and I require this manual to troubleshoot the supply rather than paying out 1000's of dollars to have the it replaced by Philips.
If anyone has this manual (or circuit diagrams) I would be very appreciative of a copy.
You can contact me offline if you wish, or at the numbers below.
Thanks in advance
Fred Pearson
******************************************************* Fred Pearson Brockhouse Institute for Materials Research McMaster University 1280 Main St. West Hamilton, Ontario Canada L8S 4M1
Has anyone received information about recertification? I have not received anything and I'm a bit concerned that my certification will expire even while working in the field.
JBozzola-at-aol.com-at-Sparc5.Microscopy.Com wrote: } } } I need to pump this group for information (sorry, it slipped out). } } } } The recent discussion of ion pump lifetimes has generated some talk here } } about the lifetime of turbo pumps. Does anyone have figures for turbos? For } } example, say one has a TP with magnetic bearings and runs the pump } } continuously, at what point would one expect some sort of problem due to } } normal wear and tear? I assume that metal fatigue would be a factor to } } consider here. } Dear John, The HVEM has 2 Balzers TPU330's, one on the accelerator and the other on the column. The accelerator is always kept at ~3*10^-7 torr, and the pump is run at ~2/3 speed in standby mode. The column is at ~7*10^-6 torr and is occasionally aired and pumped out. We have had the reccommended bearing changes at 2-year intervals (the wicks are also changed at that time) and we change the oil twice a year. We have had no problems at all with the pumps--in contrast to the old TMPs we replaced them with many years ago. Yours, Bill Tivol
SUMMARY:(To anyone interested in the details, all replies follow.)
Thanks to all for your feedback (including phone calls). I was surprised by the volume, but that helped justify to others here my concern about venting in general.
The overall view was that I had nothing to worry about in terms of pump performance and lifetime, although there were a couple of horror stories. Many responders vent to their fume hood air-handling systems, or other positive-pressure exhaust systems. What happens to the 'bad air' once discharged outside was a real concern - I have left that one to the engineers. Other concerns included handling condensation especially water coming down from the roof, and maintenance of filters.
What I did was have them use 45-degree angles where possible and include a T-cleanout at the wall for each pump.
Thanks again-- Ann Lehman
--------------------------------------------------- THE QUESTION: Dear Listers,
My Safety Officer wants all our mechanical pumps vented outdoors. The pumps are Sargent-Welch, Alcatel, and Edwards and are used to back TEMs, film desiccators, evaporators, etc. Initially I thought this was a good idea, having done it simply in past lives by drilling a hole in a wall, ganging the outlet hoses, and presto - out the bad smoke went.
However the B&G engineering consultant got hold of the project (yes now it's a 'project') and now EVERY pump must get its own line up to the roof. This means each pump's discharge is directed to a run of 1-inch copper pipe with three to five 90-degree bends over a total vertical length of about 20'. (Luckily I am on the top floor.) There is also talk about inserting a clean-out or filter at each feed-through for dealing with accumulated oil.
I'm concerned that the pumps are not designed for backpressure on the outlet side coming from the 20' column of air plus the resistence from the 90-degree bends and filter.
Could this setup affect the (1) efficiency or (2) overall life of the pumps? Am I being overly cautious?
I'd appreciate feedback from the List (including manufacturers) re the feasiblity of this approach, and the pump specs - I haven't been able to find anything about discharge 'load' tolerances.
Thanks, you can reply offline and if there is sufficient interest I'll summarize responses for the List.
Ann Hein Lehman EM Facility Mgr Trinity College Hartford, CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-exchange.cc.trincoll.edu
--------------------------------------------------- THE ANSWERS:
My guess is that this will be hard to judge. We have a much saner system with the pump exhausts fed into the air handling system (which takes exhaust air from our home in the second sub-basement to the 46th floor where it is released). When we put a filter on the exhaust, we notice no adverse effects, and as the filter becomes full of oil, the back-pressure must rise. I'd think that the air column--bends and all-- would be a small contribution compared to the filter. I would definitely reccommend having some sort of oil trap on the line, since otherwise oil would accumulate and potentially cause problems. Good luck. Yours, Bill Tivol --------------------------------------------------- Yes, please summarize. I assume you are using an exhaust filter now and this presents some impedance to the exhaust. Presumably with the copper pipe
exhause, the filter would be removed so this would save a little on the overall impedance.
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
--------------------------------------------------- If you are worried about back pressure you can put a fan in the lines to put negitive pressure at the outlet of the vacuum pumps.
Gordon Couger gcouger-at-couger.com www.couger.com/gcouger Stillwater, OK 405 624-2855 --------------------------------------------------- Although we do not manufacture these pumps we do supply the range as backing pumps for our Range of Preparation Equipment, Sputter Coaters, Carbon Coaters etc, etc. We would always recommend an Oil Mist Filter and always provide one, these can be of a type which remove the oil and just vent into the air or you can have an inline type where they remove the oil and have an outlet position which you can vent to air.
In general terms the single and dual oil filled rotary vacuum pumps will withstand back pressures of at least 1/4 of a bar i.e. approx. 4 p.s.i. which equates to 8 feet head of water back pressure. So you will have no problem with your 20 feet of air.
Trust this is of help. On behalf of Emitech Ltd. --------------------------------------------------- Unless your lab is at a partial vacuum and you are venting your pump to atmosphere, backpressure is a moot point. The gas flow is miniscule except for initial roughing.
Runs, elbows, diameters, restrictions are all critical on the INTAKE side of the pump, but have no significant effect on the outlet side of the pump.
Ken Converse owner, Quality Images Delta, PA 717-456-5491 717-456-7996 fax --------------------------------------------------- I think you are being overly cautious. The static pressure inside your 20' of pipe is, of course, the same as the pressure outside. The only worry would be dynamic pressure as you pumped down the column, dessicator, or whatever. A length of 1" pipe can handle an enormous amount of air, and you only pump at maximum rate for a few moments. After all, a small-to-medium rotary pump has a capacity of, say, 5 cu. ft. per minute, which corresponds to 8,640 cu. in per minute, or 144 cu. in pr second. Very roughly, the cross-section of your pipe is 1 sq. in., so the velocity is about 150 in/sec, or 13 ft/sec, 10 mph. The pressure drop to get this velocity will be quite small. When the chamber or whatever is evacuated, the gas flow is negligible.
The whole design plan seems like overkill, though. Does the design engineer have an interest in the plumbing company that would install the pipework? Our system at MIT vents into ducts left over from a hood formerly installed in the area, so the fan, ductwork to the roof, etc., were all in place.
tonygr-at-MIT.EDU --------------------------------------------------- I don't think back pressure is an issue but something to consider: under the right conditions the ID of the cool Cu tubing (in the building) is condensation water from outside. We know the penalty for filling our pumps with water... The work around is to exhaust into a duct of a forced air exhaust system (like a chemical fume hood). Also I recommend that at your end of the exhaust line that a "T" fitting be used & you connect to the RA. Extend the lower side of the "T" ~6 inches & terminate with a ball valve. That way if anything comes down the pipe it goes into the trap rather than your pump.
& the big one: I'd be sure the F&E (B&E) group understands the liability they expose themselves to. Unknown to us till the pump croak, our F&E guys modified our exhaust line & poured water into our Fomblin full pump.
good luck, Bruce Brinson Rice U. --------------------------------------------------- I would say you have nothing to fear. Typically, a roughing pump should be fitted with an oil mist filter. The back pressure generated by the filter would "swamp" any from the piping you described. My answer is predicated on pumping down a reasonably sized, sealed vacuum chamber with a rotorary vane pump or similar. After the initial few gulps of gas, the volume of gas moved (at STP) is very tiny. If your vacuum chamber is the size of a small room and the initial pumpdown is by something like a roots blower, that is different... {g}
Woody White McDermott Technology --------------------------------------------------- My first response would be to query whether the safety office really knows what he desires to vent with this very expensive venting project. Usually the most noxious part of a roughing pump exhaust is the particulate or oil mist. If you are not pumping something dangerous like a poison gas or something equally dreadful, I see no reason to go to the expense. The exhaust filters which have been designed for use on your pumps usually do a fine job at trapping any problem vapors. There should be no problem with back pressure. If someone finds me in error, I'm sure we will hear about it.
Good luck. Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. Number 499, Post Office Box 19400 Austin, Texas 78760 Phone: 512/282-5507 FAX 512/280-0702 --------------------------------------------------- I can sympathize with your problems. I run a plastic sump pump hose from each of my rotary pumps out to the window. If you have ever watched an untrapped rotary pump, you will notice that there is almost no air movement out of the pump after the initial ten seconds of pumping. There should be no problem with long runs, bends, etc. if there is nothing but simple atmospheric pressure to oppose the air movement. A simple drain at the first bend up should drain any condensed oil, or a wad of steel wool in the line. Use as large a diameter pipe as you can get them to run. One solution I've seen is to put a commercial car oil filter onto the pump exhaust. If this didn't bother it, neither should your copper line.
mager-at-interchange.ubc.ca --------------------------------------------------- we have 21 mechanical pumps in a total of twelve rooms in our lab. This includes pumps for specimen prep equipment, electron microscopes and desiccators much like with your situation. We remodeled the labs in 1992 and installed a new exhaust system to remove oil mists. We had previously installed some exhaust systems in the late 1970s because we had a post doc MD/Ph.D. here who insisted that breathing oil mist vapors was not healthy. I have accumulated the oil since 1992 (it condenses out into one liter containers above our pumping stations). Today I measured the total volume accumulated in all rooms. It was just a little more than one liter. This is a long time to accumulate oil, but it may be useful information for you. There are pumps on the roofs of the respective buildings which house the equipment referred to above. All roof pumps are at least 30 feet away from the instrument pumps.
We used to use the oil mist traps but we found they required a lot of maintenance with that many pumps. Cleaning them requires a lot of time and if you replace them every few months, the cost adds up quickly.
I assume that most of our accumulated oil is SUPERGRADE A OIL, the type we use for our mechanical pumps. This is produced by Inland Vacuum Industries in Churchville, NY, according to the MSDS sheets I have. These sheets also indicate the following: "VENTILATION US Gov't 8 hr TWA limit for exposure to oil mists is 5 mg per cubic meter."
I assume that some of the accumulated oil I collected is diffusion pump oil (Santovac 5). This is a polyphenyl ether and is made my Monsanto. The Permissible Exposure Limit (PEL) is also 5Mg/cubic meter for this oil.
I like our system because it is maintenance free for us. Our university air conditioning mechanics maintain the pumps on the roof.
John.Wheatley-at-ASU.Edu --------------------------------------------------- The biggest problem with that sort of a set-up is that the water vapor will condense in the exhaust line and after the pump is turned off flow back into the pump ruining it in a short time. You will need a trap for the condensation and you will have to empty it on a regular basis. The long exhaust line will not add to the efficiency of your pumps.
Hope that helps, Peter Jordan, EMSI --------------------------------------------------- I applaud the decision to vent the exhaust. I know of no EPA or other regulation to do so, but the exhaust of mechanical pumps on initial pumpdown is something I have always recommended my customers be rid of. I hope that you are also taking measures to move the pumps into separate rooms or acoustic enclosures to reduce their noise output.
Working with EMs often means spending many hours at a time living with these problems. While occasional exposure can be acceptable, the constant drone of pumps and exposure to pump oil vapors can only be detrimental.
The setup you detail presents no problem to operation of the pumps. Air is very compressable and the volume of air present in the exhaust lines means that there will be only a negligible pressure increase due to the various bends.
A clean-out filter is unnecessary. Proper design of the exhaust stack should provide for a low point where accumulated oil can be either be drained or returned to the pump. This requires less than 90 degree bends in the stack so that any condensed oil can flow back to the pump or a drain. If the exhaust provides for a return to the pump, you have to be aware that there might also be other condensed materials, primarily water, included. Best bet would be to have a slightly greater than 90 degree bend close to the pump followed by a vertical section forming a trap. At that second bend, a drain should be included that would allow for the drainage of all fluids trapped there. Any such scheme would of course require the regular emptying of the trap.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com --------------------------------------------------- All four of our mechanical pumps, those on vacuum evaporator, glow discharge, film dessicators, and TEM, have been fitted with oil mist eliminator filters on the exhaust line. I suppose there may still be some noxious fumes emitted especially at initial evacuation but our noses don't detect them. One less expensive alternative to the piping exhaust that your Safety Officer proposes might be to mount an appropriate exhaust filter on one of the pumps and have the Safety Officer monitor the output with an appropriate sensing device and compare to unfiltered output.
However, if the Safety Office budget is going to pay for the piping project...
Just a couple of thoughts. Don Gantz Boston Univ Med School --------------------------------------------------- Nothing personal, but your safety and facilities people are NUTS! But, to give you some ideas:
1) at BNL in the Light Source, the rotary-pumps exhausts are attached to a flexible corrugated-hose. This hose has a slight negative pressure. 2) Where possible, I attach rotary-pump exhaust to the "house vacuum". But, you have to monitor the "house vacuum", incase the compressor is turned off. 3) Some of our Edward's pumps have large oil-filters on the exhaust. We get these filters at the local car-parts store. They are cheaper and more massive than the ones the vacuum companies sell.
hasta, Jim jquinn-at-dol1.eng.sunysb.edu --------------------------------------------------- I have two suggestions. If these are low volume pumps 3/4" copper is probably enough for each pump. If two or more pump lines are joined then the size should be increased to maintain a cross sectional area equal to the the 3/4" time the number of pumps. Yes, traps should be incorporated into the lines to catch condensed oil and water. If the line is going out the roof you must prevent water from entering the line and it may need to be insulated to prevent water condensation on the inside only if you pump significant water.
Good Smokin, Russ RGillmeister-at-sdms.usa.xerox.com --------------------------------------------------- I think venting the MPs to the outside is a good idea. We did this in our lab by connecting the exhausts to the nearest hood using a copper pipe and rubber connectors. For our Hitachi, which has three MPs, our maintenance department built a large wooden box with a removable plexiglas lid. The lid has a large, flexible aluminum air duct connected to it. The whole thing exhausts via a hood. We can really smell the difference! So far we have had no problems.
Good luck Jordi.Marti-at-alliedsignal.com --------------------------------------------------- Only to say that if the pumps were designed to be able to operate in the described manner then the manufacturers would have specs on how and what type of ventilation system to install under different applications, i.e. extraction type if there is more than 10' of pipe to outside, or more than 2 bends totaling more than 90 degrees. Also, if discharging the "bad air" outside then EPA standards must be met...such as installing "scrubber" systems to insure no oil or certain hydrocarbons are discharged into the air and eventually being introduced into the ground water. Best thing is to install a factory offered oil recovery exhaust filter system such as the one we spoke about on the coating system. And to check with OSHA as to the "hazards Imposed" by the Vacuum Pump vapors. If they say they are too hazardous for normal laboratory environments then we may explore the possibility of routing the exhaust into existing "Fume Hoods" in the laboratories. That is assuming they have the necessary environmental "cleaning" systems built into them.
Bottom line is I doubt that without a lot of engineering the pumps will be able to be operated properly with the exhaust "routed" through any type of piping more than a few feet long. Meaning that if you hook any lines, more than a few feet long without bends or any that have more than 90 degrees of bends, to remove the fumes to any of the pumps, they won't work properly without a lot of engineering to eliminate any "back pressure" that may be caused.
--------------------------------------------------- (This is not a direct response, but a related one - In reply, I would add that the lab I used to work in had its air intake sited exactly where the delivery trucks would park and idle their engines for hours while unloading. The fumes made several people sick - nausea & headaches. So it is possible your guy suffered a hypersensitive reaction. --Ann Lehman)
Funny how we were just talking about vacuum pump oil vapours and how to deal with them... Last Tuesday I had a guy in the lab to change the locks (long, unrelated story), including the lock on the door right behind our ESEM. Our vacuum lines pass through a cut-out on this door to the pumps located in a big warehouse-like area. We have the usual little filters on the pumps, but as we all know, you can usually smell a little something in the air anywhere near them. While changing the lock, the guy was standing very close to the pumps for perhaps 10 minutes or so, and said he could smell something "oily". Well, I just found out that later that day, he became quite ill, with apparently a toxic reaction to a substance or substances unknown, and was hospitalized for a few hours, though he later, apparently, recovered completely. We're not absolutely sure that breathing our oil vapours for a few minutes is actually what caused the reaction, but it appears to be the only possible toxin the man was in contact with that day ( or at least that's the story). It's possible that the gentleman just had an unusual sensitivity to the vapours; I know at times I've had lots more exposure than he did, and have personally never gotten so much as a headache. One thing bothers me a bit, though; we use Alcatel 102, an oil specially made for ESEM's, with their increased water throughput, and I wonder if this stuff might be a bit less user-friendly than the more common types. Has anyone else out there ever seen, or heard of, a toxic reaction to this or any other pump oil? (I have a funny feeling that I haven't heard the end of this incident yet...)
F.C. Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
Does anyone know of any short courses given in New Jersey related to=20 Failure Analysis, Metallurgy, Metallography, Etc=2E? If so, I would=20 greatly appreciate the information=2E =20 TIA, =20 Leslie Link e-mail: Leslie=2ELink-at-us=2Egtc=2Eboc=2Ecom
I have an OLD razor blade holder with plastic anti-roll plate. There is IEC engraved on it. Could this be what you are looking for? Lilith ------------------------------------------------------ Lilith Ohannessian-Barry National Research Council Institute of Biological Sciences CANADA Tel;613-993-6460 Fax;613-941-4475 e-mail; lilith.barry-at-nrc.ca ---------- } From: "COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com To: microscopy-at-sparc5.microscopy.com -----------------------------------------------------------------------.
In a message dated 4/9/99 6:14:32 PM US Mountain Standard Time, walck-at-ppg.com writes:
} microscopy-at-sparc5.microscopy.com
We have a most ancient AO microtome with cryostat and a dull ugly blade in a wood box. It would be neat if we could find one of the holders that held the razor blades ... any floating out there??! Ed Sharpe
Check with your local Mattel toy store for the recently released "Play X3 Digital Video Microscope" with a list price of $99. This is a joint venture with Intel and is CMOS based. It is designed to let children of all ages display images from the microscope on a PC at both still and video rates, print images and email them. Mattel/Intel will also use similar technology for their Me2Cam. Naturally the results will not compare with the $100,000. instrumetns we use in research or will they? Larry D. Ackerman Lily & Yuh Nung Jan Laboratories Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Ave. San Francisco, CA 94143
I know you all have been through this subject before, so I thought I'd ask what month's archives would be best to search for your prior discussions on the good,bad and ugly of film scanners.
I'm responsible for near 60 years of 4x5 negatives from our R&D work here at TIMET, some of the oldest are 4x5 TEM and view camera negs but the majority are Polaroid negatives.
Perhaps, someone archived just the appropriate responses?
Any help or discussion would be greatly appreciated.
I am experimenting with phosphorous doped silicon. The goal is to determine dopant concentrations in the doped regions between the source/gate and gate/drain. I believe that with the correct recipe, the doped silicon will etch at a faster rate than the undoped regions, revealing thickness fringes when view in the TEM in the WBDF configuration. Thus far I have not obtained the desired results, ie, the etchants seem to attack all areas equally. Do you know of a wet chemical etch recipe which would achieve this result?
I was wandering if anyone could help me out as i am new to this technique. i have been retorgradely labeling cells in the spinal cord with CTB-HRP in rats and then staining with TMB and DAB reactions. however this has not been highly succesfull..any suggestions? i think the problem might be in the injections rather than the staining from what i gather under EM i should be looking for a black pepper like precipitate and some form of tungstate crystals? any suggestions on how to get dendritic staining? Thanks Jennifer Wilson
Jennifer Wilson Neurosciences Loeb Research Institute Ottawa Civic Hospital 751 Parkdale Avenue Ottawa, Ontario, K1Y 4E9 Canada Tel;( 613) 798-5555 x6387
Robert, I do not know of any selective etchant for this process, although they may exist. Stains can be used to identify n vs. p regions using optical microscopy, although this gives no quantitative data. I would probably do some C-V measurements (Capacitance-Voltage) to get at the doping. Or if these are big devised, a 4-point probe would do the trick. -good luck -andrew ___________________________________ I am experimenting with phosphorous doped silicon. The goal is to determine dopant concentrations in the doped regions between the source/gate and gate/drain. I believe that with the correct recipe, the doped silicon will etch at a faster rate than the undoped regions, revealing thickness fringes when view in the TEM in the WBDF configuration. Thus far I have not obtained the desired results, ie, the etchants seem to attack all areas equally. Do you know of a wet chemical etch recipe which would achieve this result?
Thank you,
Robert Pomrenke bpom-at-aol.com
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_ 10:45 =F1 11:30 : "Future FIBs (Focused Ion Beams)=EE, Jon Orloff, Department of Electrical Engineering and Institute for Plasma Research, University of Maryland, College Park, MD.
_ 11:30 =F1 12:15 : "Practical Applications of Focused Ion Beam (FIB) Systems=EE, Pete Carleson, FEI Company, Hillsboro, OR.
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_ 2:00 =F1 3:15 : "Strategy and Tactics of (Microprobe) Analysis=EE - Pau= l =46. Hlava, Sandia National Labs, Albuquerque, NM. --------------------------------------------------------------------------- ------- =46or more information please contact either:
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We have inherited an OLD RCA TEM minus the HVPS. I have what I think is the serial number (the ID plate is probably on the HVPS). We have no intention of trying to make it operational (we will clean it up and put it in static display), but I would like to find out the model number, about how old it is, and whatever else I can dig up on it. I used an old RCA 4 (I think) back in about '72 and that was a modern instrument compared to this one. I know RCA quit making TEM's a LONG time ago, but can anybody point me in the right direction to begin getting some info on this scope.
Thanks, Bill -- ============================================================= Bill Chissoe III Electron Microscopist University of Oklahoma 770 Van Vleet Oval Norman, Ok. 73019 E-mail: wchiss-at-ou.edu Ph. (405)325-4391 =============================================================
} I am looking for sources to obtain a quality "student" grade microscope for } my son, who is 10 years old. Can you or the MSA offer an recommendations or } guidance? } Bill -
You'll find detailed advice on what to buy, plus a list of suppliers, on the Project MICRO website (URL below).
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Howdy Folks, } } I know you all have been through this subject before, so I thought I'd ask } what month's archives would be best to search for your prior discussions on } the good,bad and ugly of film scanners. } } I'm responsible for near 60 years of 4x5 negatives from our R&D work here at } TIMET, some of the oldest are 4x5 TEM and view camera negs but the majority } are Polaroid negatives. } } Perhaps, someone archived just the appropriate responses? } } Any help or discussion would be greatly appreciated. } } Bill } TIMET
Since the 2000FX was referred to in recent discussions regarding sputter ion pump lifetimes, I thought it pertinant to add a few items that can have an effect on the life of the pump with this instrument and others as well.
Of course the basis for pump life has alot to do with how tight the chamber it pumps on. If ultimate pressure is near 1 X 10 -5 Pa you can safely assume the gun/column area is reasonably tight. An order of magnitude or so higher and you need to find the vacuum leak (or replace the pump). A simple leak rate check will verify gun/column integrity.
Here at ASU we got 9 & 1/2 years use from the orginal ion pump. The reason why has alot to do with the above. However there are other reasons to consider. One being we have religiously used the cold trap (or ACD). If this is cold prior to sample insertion, the vacuum recovery time after insertion is remarkably quicker as the admitted gases are trapped immediately and thus saved from the ion pump. When baking the ACD at the end of the day, the pumping configuation is changed (in ACD HEAT mode)to where the ion pump is shut off as the trap is heated. A normally closed valve opens and the trapped contamination is released and diffusion- pumped from the system. Incorporating this system of trapping, heating, and diffusion pumping contamination from the microscope will extend the life of the pump as well as keep the contamination rate minimal in the beam/specimen interaction area.
Bob Roberts Arizona State University Center for Solid State Science PSA-213 Tempe, Arizona 85287-1704
Bill Chissoe III wrote: ================================================== We have inherited an OLD RCA TEM minus the HVPS. I have what I think is the serial number (the ID plate is probably on the HVPS). We have no intention of trying to make it operational (we will clean it up and put it in static display), but I would like to find out the model number, about how old it is , and whatever else I can dig up on it. I used an old RCA 4 (I think) back in about '72 and that was a modern instrument compared to this one. I know RCA quit making TEM's a LONG time ago, but can anybody point me in the right direction to begin getting some info on this scope. ================================================ If it is not an EMU-4 (e.g. A, B, or C), then it would have been produced by RCA in early 1969 or before, that being the time they introduced the EMU 4-A . That means it probably would be an EMU3 A, B, C,....or H. The first production of the EMU3 series would have started in the early 1960's (if I remember correctly).
You might want to contact Prof. Arthur Smith of West Chester University. He has some number of these older RCA EMU-3's in operation which he uses in conjunction with some very effective courses on EM maintenance. His e-mail address is asmith2-at-wcupa.edu
He and his students service all of these ancient RCA TEMs and Prof. Smith probably is the leading remaining authority on anything related to these old instruments.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
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Fred Pearson wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello Everyone: } } I operate and service our Philips CM12. The +15 volt power } supply in the remote racks has failed, mainly in the area of the 2 } MJH-16010 transistors. } } The designation for this supply is PE 1130/00 and I require this manual to } troubleshoot the supply rather than paying out 1000's of dollars to have } the it replaced by Philips. } } If anyone has this manual (or circuit diagrams) I would be very } appreciative of a copy. } } You can contact me offline if you wish, or at the numbers below. } } Thanks in advance } } Fred Pearson } } ******************************************************* } Fred Pearson } Brockhouse Institute for Materials Research } McMaster University } 1280 Main St. West } Hamilton, Ontario } Canada L8S 4M1 } } email: eoptics-at-mcmaster.ca } phone: (905) 525-9140 ext. 24609 } fax: (905) 521-2773 } ******************************************************** Fred,
You may also use a number of alternative sources for spare parts. In this particular case I recommend switching power supply made by ETA POWER, LTD. Power supply part # 618-FHP15SX, rated 15V 32A (original Philips one rated 15V 25A). You can order it from MOUSER ELECTRONICS, (800)346-6873. Order number 33R42, price- $495, warranty- 3 years (not bad). I used ETA supplies more than once with great success. These are fully protected top of the line units. It is possible, of course, to use much less expensive replacement if you do not care about warranty. Contact me off line shall you have any questions.
Vitaly Feingold SIA, Inc. (770)232-7785 office (770)605-6105 mobile
Polaroid SprintScan 45 is probably the best for 4x5 negs or chromes. It is much more compatable with a Mac than with a PC however. It requires a simple slow speed narrow SCSI bus.
gary g.
At 12:33 PM 4/13/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
RCA actually was, I believe, the leading manufacturer of TEMS in America in the early days.
Ed Sharpe
} Subj: old tem } Date: 4/13/99 5:48:16 PM US Mountain Standard Time } From: wchiss-at-ou.edu (Bill Chissoe) } Reply-to: {A HREF="mailto:wchiss-at-ou.edu"} wchiss-at-ou.edu {/A} } To: microscopy-at-sparc5.microscopy.com (Microcoscpy List Server) } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We have inherited an OLD RCA TEM minus the HVPS. I have what I think is } the serial number (the ID plate is probably on the HVPS). We have no } intention of trying to make it operational (we will clean it up and put } it in static display), but I would like to find out the model number, } about how old it is, and whatever else I can dig up on it. I used an old } RCA 4 (I think) back in about '72 and that was a modern instrument } compared to this one. I know RCA quit making TEM's a LONG time ago, but } can anybody point me in the right direction to begin getting some info } on this scope. } } Thanks, } Bill } -- } ============================================================= } Bill Chissoe III } Electron Microscopist } University of Oklahoma } 770 Van Vleet Oval } Norman, Ok. 73019 } E-mail: wchiss-at-ou.edu Ph. (405)325-4391 } ============================================================= } } } } } } ----------------------- Headers -------------------------------- } Return-Path: {Microscopy-request-at-sparc5.microscopy.com} } Received: from rly-yb03.mx.aol.com (rly-yb03.mail.aol.com [172.18.146.3]) } by air-yb01.mx.aol.com (v59.4) with SMTP; Tue, 13 Apr 1999 20:48:16 -0400 } Received: from Sparc5.Microscopy.Com (sparc5.microscopy.com [206.69.208.10]) } by rly-yb03.mx.aol.com (8.8.8/8.8.5/AOL-4.0.0) } with SMTP id UAA10462; } Tue, 13 Apr 1999 20:48:04 -0400 (EDT) } Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) } id RAA12287 for dist-Microscopy; Tue, 13 Apr 1999 17:23:07 -0500 } Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by Sparc5. } Microscopy.Com (8.6.11/8.6.11) with SMTP id RAA12281 for " } MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 13 Apr 1999 17:22:35 -0500 } Received: from hermes.services.ou.edu (hermes.services.ou.edu [129.15.2.121]) } by Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id RAA12272 for { } microscopy-at-sparc5.microscopy.com} ; Tue, 13 Apr 1999 17:22:22 -0500 } Received: from styx.services.ou.edu by hermes.services.ou.edu with ESMTP; } Tue, 13 Apr 1999 17:19:23 -0500 } Received: from ou.edu (botchiss.bio.ou.edu [129.15.38.205]) } by styx.services.ou.edu (8.9.1/8.9.1) with ESMTP id RAA19617 } for {microscopy-at-sparc5.microscopy.com} ; Tue, 13 Apr 1999 17:19:22 -0500 ( } CDT) } Message-ID: {3713C2B2.5CF8BD6B-at-ou.edu} } Date: Tue, 13 Apr 1999 16:18:26 -0600 } From: Bill Chissoe {wchiss-at-ou.edu} } Reply-To: wchiss-at-ou.edu } Organization: University of Oklahoma } X-Mailer: Mozilla 4.06 [en] (Win95; I) } MIME-Version: 1.0 } To: Microcoscpy List Server {microscopy-at-sparc5.microscopy.com} } Subject: old tem } Content-Type: text/plain; charset=us-ascii } Content-Transfer-Encoding: 7bit } Errors-to: Microscopy-request-at-sparc5.microscopy.com } }
I have found that SEMs decrease in value following a curve like a piano off a cliff.
A JEOL 840 or other similar SEM is probably worth (going price) about $10K-$15K. Just about any other SEM that is 7-9 years old is also worth about the same amount. SEMs older than that are of course worth less. "Worth" is an interesting word in this context.
Most companies depreciate their capital equipment over 8-9 years. Thus, you would see SEMs offered that are 8-9 years old. According to the company, the equipment has been depreciated to zero value. Therefore, the salvage value, if greater than zero, might actually cost them money in recouping depreciation amounts. consequently, one sees offers to "take this thing away" or some such freebee.
The flip side is that if a person has a SEM that is working and is on-line, that makes a big difference between one that is not or is not covered by a maintenance contract. It is not unusual for an organization to stop maintenance on a SEM several years before the depreciated life is up. Then, it is caveat emptor.
Please send me a picture of this I will check the archives.
One curious TEM RCA made actually was a small unit that sat atop the bench I saw one in a microbiology for nurses manual I have. Does anyone out there have one of those? I would be interested in it for the museum. thanks, Ed Sharpe
We have inherited an OLD RCA TEM minus the HVPS. I have what I think is the serial number (the ID plate is probably on the HVPS). We have no intention of trying to make it operational (we will clean it up and put it in static display), but I would like to find out the model number, about how old it is, and whatever else I can dig up on it. I used an old RCA 4 (I think) back in about '72 and that was a modern instrument compared to this one. I know RCA quit making TEM's a LONG time ago, but can anybody point me in the right direction to begin getting some info on this scope.
Thanks, Bill -- ============================================================= Bill Chissoe III Electron Microscopist University of Oklahoma 770 Van Vleet Oval Norman, Ok. 73019 E-mail: wchiss-at-ou.edu Ph. (405)325-4391 =============================================================
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That is amazing!!! Keep the software and the camera part, throw away the microscope and use on any larger microscope. I was considering getting one of the Mattel Barbie cameras for $69 to gut for the active electronics. Actually I was going to get 2 of them one for permeant mounting to a microscope and the other I was going to kluge into one of my old Nikon F bodies...... Ed Sharpe
} Subj: Student Microscopes } Date: 4/13/99 3:03:09 PM US Mountain Standard Time } From: mishot-at-itsa.ucsf.edu (Larry) } To: microscopy-at-sparc5.microscopy.com } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Check with your local Mattel toy store for the recently released "Play X3 } Digital Video Microscope" with a list price of $99. This is a joint venture } with Intel and is CMOS based. It is designed to let children of all ages } display images from the microscope on a PC at both still and video rates, } print images and email them. Mattel/Intel will also use similar technology } for their Me2Cam. Naturally the results will not compare with the $100,000. } instrumetns we use in research or will they? } Larry D. Ackerman } Lily & Yuh Nung Jan Laboratories } Howard Hughes Medical Institute } UCSF, Box 0725, Rm U226 } 533 Parnassus Ave. } San Francisco, CA 94143 } } (415) 476-8751 FAX (415) 476-5774 } mishot-at-itsa.ucsf.edu }
Just a reminder please post messages to the server to the address
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I would like to get a new Critical Point Drier. Would you be kind enough to share your opinions on and experiences with different models ? So far, I am in favour for a EMS 850 CPD, but nobody here knows anything about this model.
You are welcome to write me off-line if you would like me to treat your reply confidential.
Yours sincerely
PETER FUNCH Assistant Professor, Ph.D.
Dept. of Zoology - Institute of Biological Sciences University of Aarhus Universitetsparken - Building 135, DK-8000 Aarhus C - Denmark Phone: + 45 8942 2764 - fax: + 45 8612 5175 E-mail: peter.funch-at-biology.aau.dk *************************************************************
Bill, Why not hook it up and get it running, at least to some demonstrable extent. One of the worst things about renovating an older instrument is finding a place to put it. If you have a location, I recommend pursuing the necessary facilities for operation. Put your graduate students to work. A great opportunity for them. Jerry ______________________ Jerome D. Schick, Ph.D. Semiconductor Devices and Electron Microscopy 26 Kuchler Drive LaGrangeville, NY 12540 Bus (914)223-7393 FAX (914)227-2743 jdschick-at-worldnet.att.net
COURYHOUSE-at-aol.com-at-Sparc5.Microscopy.Com wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We have inherited an OLD RCA TEM minus the HVPS. I have what I think is } } the serial number (the ID plate is probably on the HVPS). We have no } } intention of trying to make it operational (we will clean it up and put } } it in static display), but I would like to find out the model number, } } about how old it is, and whatever else I can dig up on it. I used an old } } RCA 4 (I think) back in about '72 and that was a modern instrument } } compared to this one. I know RCA quit making TEM's a LONG time ago, but } } can anybody point me in the right direction to begin getting some info } } on this scope. } } } } Thanks, } } Bill } } -- } } ============================================================= } } Bill Chissoe III } } Electron Microscopist } } University of Oklahoma } } 770 Van Vleet Oval } } Norman, Ok. 73019 } } E-mail: wchiss-at-ou.edu Ph. (405)325-4391 } } ============================================================= } } } } } }
ALL ARE WELCOMED TO ATTEND CMS members-please let everyone know because our listserver is down and this will be the only announcement before you receive the newsletter! } "SPRING DINNER MEETING: APRIL 27" } } This year we have decided that instead of the winter conference we would } have an additional dinner meeting. It is with great pleasure that we } have Dr. Jennifer Lippincott-Schwartz talking to use this spring at } second dinner meeting about membrane trafficking and protein transport } in the cell using GFP tagged proteins. } } Place: Tia Queta } 4839 Del Ray Ave. } Bethesda, MD } 301-654-4443 } } Date: April 27, 1999 (Tuesday) } } Time: 6:00 - 6:30 appetizers } 6:30 - 7:30 dinner: } red snapper,tenderloin and chicken } 7:30 - 8:30 speaker } } Cost: $25/person, $15/student } } RSVP by April 24 to: Jenny Hinshaw } 301-594-0842 } jennyh-at-helix.nih.gov } } Directions: From 495 take 355 or Wisconsin Ave exit off } 495 and head south toward Bethesda. After you pass NIH follow the } directions below. } } From NIH take Wisconsin Ave south to Woodmont Ave. Take a right } onto Rugby (soon after you veer right onto Woodmont) and then an } immediate left onto Del Ray. The restaurant is down a block on the } right. If the street parking is filled, they have valet parking for $3 } or public parking further down (a block) on Del Ray on the right. Also } there is public parking on Woodmont right before you turn onto Rugby. } } } } }
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i do very low volume CPD work, and have had good luck with a samdri pvt-3b manually operated system. it was cheap and seems to be pretty rugged, and most importantly, easy to operate.
good luck
b-
**************************************************************** Brian McIntyre Electron Microscopy Lab Institute of Optics University of Rochester Rochester, NY 14627
716-275-3058 716-244-4936(fax)
"You may get to the top of the ladder of success only to find its been leaning against the wrong wall" A. Raime
Lately there has been a lot of responses about Scanners for TEM negatives and they seem to have all been centering around the flat-bed type. Does anybody have any comments about drum scanners, for example the JEI eX4, or the Imacon Inc. FlexTight Precision II? I know that typically drum scanners are more expensive as a rule, but do any of you use these? We are considering something along those lines. We currently have the LeafScan45, but it is feeling it's age and we want to be prepared for it's eventual end.
Thanks in advance for your comments.
Peggy Bisher.
Margaret E. Bisher
NEC Research Institute 4 Independence Way Princeton, NJ 08540. Tel.: (609) 951-2629 Fax: (609) 951-2496 e-mail: peggy-at-research.nj.nec.com
} ...the Imacon Inc. FlexTight Precision II?...do any of you use these?...
A collaborator and I had been using an ancient Perkin-Elmer scanning microdensitometer (circa late 70's) which provided 2500 dpi and gave good images, but we grew weary of its slowness (~30 min/ for a 1" x 1" scan) and lack of support. We compared the output of the Imacon with that of the Perkin-Elmer and a Polaroid Sprintscan and were quite pleased with the Imacon, so my collaborator bought one. He has since moved to Richmond, and as I have not heard otherwise, I presume that the Imacon is performing well.
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Larry -
I don't have a "local toy store" here on the coast, but I need to check this out immediately for Project MICRO. Can you supply me with any kind of contact info? Have you seen this thing?
Caroline
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
We have just had a cold stage installed on our SEM capable of obtaining near -200C. Although our intentions are primarily for cathodo-luminescence and would enjoy discussing other issues and techniques, this e-mail primarily addresses sample preparation and mounting standard thinsections on the cold stage.
The current specimen hold offers too little area for even a 1" round TS, so we have in mind machining a platform of polished brass. The question here is with respect to the glass slide and holder. That is, is there a viscous medium which will hold the TS in place and provide good thermal conductivity? ... and at the same time be easily cleaned for care of the carbon coat.
If a thinsection were to be made from scratch with the cold stage in mind, is there an especially thermally conductive epoxy?
TIA and cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
You will see more on CMOS cameras. There was an ad, in Advanced Imaging I believe, which I can't find now, that offered CMOS cameras for about $50 in bulk.
In the meantime, www.supercircuits.com offers CCD "spy" cameras for about $150.
Leonard Corwin Research Chemist Fort Dodge Animal Health Princeton, NJ 08543-0400
We have a Reichert-Jung 2050 microtome and it needs service. Can anyone provide me a phone number or an email address of a service company in or near Oklahoma state so that I can contact them. Thanks
X.S. Ding Plant Biology Division The Noble Foundation Ardmore, OK 73401 Tel. 580 223-5810
I am upgrading from this 1600T to an 1830 and have priced this workhorse to sell. It is a turbo pump unit with ion pump to handle W or LaB6 emitters (both heads are included). A vibration isolation platform is also included. The scope is on-line and operational. You can see it listed on LabX at:
http://www.labx.com/aref.cfm?ad=19348
with an advertised price higher than my current value.
There are numerous pictures at http://www.gaugler.com/1600T
Dear Colleagues: } } The Core EM Facility at Dana Farber Cancer Institute ( Boston, MA, } USA) has the following equipment available immediately: } } 1. JEOL JEM-100 CXII transmission electron microscope, Side-entry } w/power supply, pumps, and water chiller. } } 2. JEOL JEM-100 CXII transmission electron microscope, Top entry } w/power supply, pumps, and water chiller. } } 3. JEOL JSM-35 CF scanning electron microscope } } 4. Reichert-JUng Ulrtacut E ultramicrotome w/FC4D cryochamber } attachment, Nitrogen tank, vibration-free table. } } 5. MT 5000 Sorvall ultramictotome with cryoattachment. } } 6.Ladd critical point dryer } } 7.Polaron SEM coating system } } 8.Durst Laborator s -45 special enlarger system } } 9.Agfa 3700 printing machine } } All items are in excellent working conditions. Please contact } Dr.Yuhui Xu by email. No telephone call please. } } Regards, } } Yuhui Xu, MD,PhD } EM Core, DFCI, } Boston, MA 02115 }
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} ... in a container eventually on its way to Taiwan as scrap mental
Hey, great! Does Taiwan really take this stuff? I have file drawers and file drawers full of scrap mental I ought to get rid of. Don't think any of it's actually toxic, but some of it was really dumb.
Now if I could only rid my brain of all those beer and soap jingles from the 60's and 70's life would be grand indeed.
MME offers customized, on-site workshops in these areas. Give me a call or email me directly and I can provide details.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 01:52 PM 4/13/99 -0500, leslie.link-at-US.GTC.BOC.COM"-at-Sparc5.Microscopy.Com wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Bottom line: there is no comparison. While the two sound similar in functionality, the optical and electronic resolution and contrast are not even in the same universe. Then we could talk about image size, transfer speed, etc.
We have a unique and challenging situation rising to critical level here: As microscopists, we need to know how our instruments work (do you know that most pathologists, for example, don't even know what Koehler illumination is, yet alone how to do it?!) as well as the intricacies of our own scientific disciplines. Now we also need to become fluent in the electronics behind cameras and all the hardware/software issues involved in computer technologies. Sorry guys, but as much as the manufacturers are trying to make things simple and "transparent to the user", we need at least a basic understanding of the principles behind each of the components in our systems. I am in the process of writing an article on "Microscopy for the Next Millenium" and the research for that article has pointed up this need, in spades!f
For those of you who could use a refresher on basic principles in microscopy and digital imaging, a number of these issues are addressed in "Optimizing Light Microscopy". Details are on our website.
As always, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 12:11 PM 4/13/99 -0700, Larry wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
MME offers customized, on-site workshops in these areas. Give me a call or email me directly and I can provide details.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 01:52 PM 4/13/99 -0500, leslie.link-at-US.GTC.BOC.COM"-at-Sparc5.Microscopy.Com wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} ---------- } From: McLean, Dorrance } Sent: Thursday 715, 24199997 8:37 AM } To: 'Microscopy Listserver' } Subject: TEM Samples of Titania } } Dear All, } } I'm in need of some advice regarding a sample I've been asked to prepare. } The sample is Ti O2 and I have been trying to get a good thin sample for } viewing in the TEM. } } So far I've had a lot of cracking during disc cutting and dimpling. I've } learned that it's better to thin the sample to about 300 microns prior to } using the disc cutter and I'm getting good results with that. I then thin } the sample to around 150 microns using diamond films. (only from the } backside as there is a film on the sample.) I have also found from trial } and error that the Bronze wheel on the dimpler causes too much damage to } the sample so I've tried thinning with the Felt polishing wheel and } diamond paste but it's hard to measure the dimple progress. I have } stopped thinning at around 30-40 microns as the thinner samples have all } cracked on the dimpler. } } I have managed to get two samples into and out of the PIPS. Of course the } "perfect" sample took a suicide leap from between the jaws of my tweezers } just as I was loading it into the specimen holder of the microscope...so } I'll never know just how perfect it was. The second sample was amorphous } at the thin areas and I suspect that (1.) I may not have removed all the } glue from the sample, or (2.) maybe the sample was too thick and sputter } was redeposited onto the sample. } } Either way I'm stumped and I would sure welcome some advise. } } Thanks for your time. } Dorrance } }
} From Microscopy-request-at-sparc5.microscopy.com Thu Apr 15 13:57:33 1999
} Date: Thu, 15 Apr 1999 13:26:59 -0400 } To: Larry {mishot-at-itsa.ucsf.edu} , {microscopy-at-Sparc5.Microscopy.Com} } From: Barbara Foster {mme-at-map.com} } Subject: Re: Student Microscopes } } Hi, } } Bottom line: there is no comparison. While the two sound similar in } functionality, the optical and electronic resolution and contrast are not } even in the same universe. Then we could talk about image size, transfer } speed, etc. } } We have a unique and challenging situation rising to critical level here: } As microscopists, we need to know how our instruments work (do you know } that most pathologists, for example, don't even know what Koehler } illumination is, yet alone how to do it?!) as well as the intricacies of } our own scientific disciplines. Now we also need to become fluent in the } electronics behind cameras and all the hardware/software issues involved i } computer technologies. Sorry guys, but as much as the manufacturers are } trying to make things simple and "transparent to the user", we need at } least a basic understanding of the principles behind each of the component } in our systems. I am in the process of writing an article on "Microscopy } for the Next Millenium" and the research for that article has pointed up } this need, in spades! } } For those of you who could use a refresher on basic principles in } microscopy and digital imaging, a number of these issues are addressed in } "Optimizing Light Microscopy". Details are on our website. } } As always, } Barbara Foster } }
Barbara, I couldn't agree with you more. I work in the cathodoluminescence area and am amazed to see how many potential users understand all about color videos and associated computer wizardry but when you start to talk about the beam power hitting the sample and possible implications of heating and sample deterioration, they don't know what I'm talking about. And when you bring up beam power per unit area (beam power density) it can really draw a blank. It's too easy to accept that everything is fine just because a pretty picture emerges. The same thing occurs in mass spectroscopy. A set of notes on Fundamentals of Mass Spectroscopy that I wrote back in the mid 1980s make almost no mention of computers but they do discuss the details of electron impact and why one often uses a 70 electron volt energy for the electron beam, etc.; Many users are astonished that they might need to worry about occasionally checking or, heaven forbid, setting such variables. But almost universally when people do see this basic physics information available in a readable format in these notes, they are very receptive and seize upon it.
Don Marshall
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology "A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)
I have not been able to track down the Mattel microscpe yet. It is not on their website, the local Toys R Us store does not have it and the Mattel Merchandising Manager does not return my calls. I assume this is another example of publicity preceding product. I saw press releases in the San Francisco Examiner newspaper some time ago (?March) and last week in the new issue of Advanced Imaging. When I locate the actual product I'll post more information.
Larry D. Ackerman Lily & Yuh Nung Jan Laboratories Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Ave. San Francisco, CA 94143
Absolutely no disc cutter. Nor or at most just a little bit dimpler. A disc-grind holder will work. Specifically, you grind any irregular piece down to as close to a thickness of {=20um as possible with the help of the holder. Break it using, for example, a hammer. Pick up a decent piece or several pieces with maximum diameter(s) {3mm, and glue it(them) to a(several) single-slot grid(s) using, say, regular SEM carbon paint or silver paint. And then put the specimen on your PIPS miller. Usually, you should mill for {10 hours. } 10 hour milling time may quite possibly indicate a preparation failure. There is another method called small-angle-cleavage method you may want to try. But that's a territory of Dr. Walck Scott ("Walck. Scott D." {walck-at-ppg.com} ). You can contact him directly. Good luck.
-cy
On Thu, 15 Apr 1999, McLean, Dorrance wrote:
} Date: Thu, 15 Apr 1999 12:07:33 -0600 } From: "McLean, Dorrance" {dmclea-at-sandia.gov} } To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com} } Subject: FW: TEM Samples of Titania } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } ---------- } } From: McLean, Dorrance } } Sent: Thursday 715, 24199997 8:37 AM } } To: 'Microscopy Listserver' } } Subject: TEM Samples of Titania } } } } Dear All, } } } } I'm in need of some advice regarding a sample I've been asked to prepare. } } The sample is Ti O2 and I have been trying to get a good thin sample for } } viewing in the TEM. } } } } So far I've had a lot of cracking during disc cutting and dimpling. I've } } learned that it's better to thin the sample to about 300 microns prior to } } using the disc cutter and I'm getting good results with that. I then thin } } the sample to around 150 microns using diamond films. (only from the } } backside as there is a film on the sample.) I have also found from trial } } and error that the Bronze wheel on the dimpler causes too much damage to } } the sample so I've tried thinning with the Felt polishing wheel and } } diamond paste but it's hard to measure the dimple progress. I have } } stopped thinning at around 30-40 microns as the thinner samples have all } } cracked on the dimpler. } } } } I have managed to get two samples into and out of the PIPS. Of course the } } "perfect" sample took a suicide leap from between the jaws of my tweezers } } just as I was loading it into the specimen holder of the microscope...so } } I'll never know just how perfect it was. The second sample was amorphous } } at the thin areas and I suspect that (1.) I may not have removed all the } } glue from the sample, or (2.) maybe the sample was too thick and sputter } } was redeposited onto the sample. } } } } Either way I'm stumped and I would sure welcome some advise. } } } } Thanks for your time. } } Dorrance } } } } } }
Whoever finds one of these new wunderkund toys-- please post an image on your website so we all can observe what kind of image the next generation of microscopists is seeing.....
} I have not been able to track down the Mattel microscpe yet. It is not on } their website, the local Toys R Us store does not have it and the Mattel } Merchandising Manager does not return my calls. I assume this is another } example of publicity preceding product. I saw press releases in the San } Francisco Examiner newspaper some time ago (?March) and last week in the } new issue of Advanced Imaging. When I locate the actual product I'll post } more information. } } } Larry D. Ackerman } Lily & Yuh Nung Jan Laboratories } Howard Hughes Medical Institute } UCSF, Box 0725, Rm U226 } 533 Parnassus Ave. } San Francisco, CA 94143 } } (415) 476-8751 FAX (415) 476-5774 } mishot-at-itsa.ucsf.edu
--------------------------------------------------------------------- Dr. Steven Barlow, Associate Director EM Facility/Biology Department San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619)594-4523 fax: (619) 594-5676 email: sbarlow-at-sunstroke.sdsu.edu website: http://www.sci.sdsu.edu/emfacility/
Chairman, Educational Outreach subcommittee promoting access to microscopes Microscopy Society of America http://www.msa.microscopy.com/
I have a Brinkmann AS2 microscope I would like to know more about. It appears to be Leitz or a copy of one. Every thing on it has matching serial numbers and the oculars are marked 10x pl. It looks like it was made in the late 50's or early 60's. Talking to Brinkmann they say it was made for them by Zeiss. Talking to Ziess they say they never made scopes for anybody but themselves. It does say made in Germany.
The field is very flat and I don't see any distortion on the edge so I expect the pl mean plan optics. The oil immersion lens is a 160 100/0/1.3.
Pictures or it are at www.couger.com/auction/scope.
I bought the scope because I have a triocular head that fits it.
If anyone could help me with more information about the scope or copy of a manual I would pay unreasonable copying and shipping cost.
Thanks Gordon
Gordon Couger gcouger-at-couger.com www.couger.com/gcouger Stillwater, OK 405 624-2855 GMT -6:00
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Actually, the brand and model of SEM has some degree of influence over the depreciated value of the scope. For example: Hitachi: 500, 520, 570 are boat anchors. Hitachi: 800, 900 are FE scopes and are more modern. Hitachi: 2960 and 3000+ are really more modern and can include the Nature option and are very good scopes.
A JEOL 840 series is good.
Philips? maybe a XL40FE. But as with any field emission scope, one must be willing to put up with the extra grief of FE over LaB6. Yet, there is an image intensity benefit. It is a tradeoff.
gg
At 10:03 PM 4/15/99 , you wrote: } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } The point you make here, of course, is quite correct. Our fifteen year old } Model 840, set up and working, in our laboratory, has far more value to us } than what is showing on our books. } } With regard to the depreciation of AMRAY instrumentation, it sounds like } those instruments might be depreciating faster than say, JEOL or Philips } equipment. } } Chuck } -------- REPLY, Original message follows -------- } } } Date: Thursday, 15-Apr-99 06:17 PM } } } } From: Dr. Gary Gaugler \ Internet: (gaugler-at-calweb.com) } } To: Garber, Charles A. \ Internet: (cgarber-at-2spi.com) } } } } Subject: Re: Value of SEM } } } } At 12:12 AM 4/15/99 , you wrote: } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } } } } } Hi Gary: } } } } } } I think that you have to make some distinction between the a) depreciated } } } value vs. b) market value. There two can be very much different. } } } } } } We depreciate most of our equipment over five years, our computers we } } } depreciate even faster. After five years it has zero value on our books. } } } } But if you wanted to purchase it from us, we would seek to charge you } full } } } market value. And if it meant there was some recovery of earnings, then } we } } } would pay taxes on those recovered earnings. } } } } } } But that itself have meaning only if the company has earnings on which } taxes } } } have to be paid. If a firm is operating at a loss, then when they } recover } } } those funds, they do not pay any taxes. And even a highly profitable } } } company like DuPont or IBM often times try very aggressively to sell } their } } } depreciated equipment for as much as they can get for it, and to whatever } } } degree they might recover assets, they pay their taxes, but are still } better } } } off than they would be had they just given it away. } } } } } } I do know however of certain companies that will give away equipment } rather } } } than selling it, under the premise that if someone is injured at some } later } } } date, they would then have less exposure to those claims. } } } } } } Chuck } } } } } } Each company probably has a different cost/benefit scenario and thus a } } different view of the salvage value of a SEM at the end of the } depreciation } } period. Nevertheless, my point was that SEMs drop in value really fast. } And } } by about 6-8 years, they are near worthless in monetary terms. The cost } of } } takedown, setup and moving overshadow the actual cost of the machine. I } just } } bought an Amray 3600LEAP that is 1.5 years old for $150K when the new } price is } } $422K. I also bought an 8 year old Amray 1830 for $8K. In each case, the } cost } } of take down and setup by Amray is about $7K. The cost of moving is about } } $3500 each. So for the older unit, the handling and shipping is more than } cost } } of the instrument. } } } } } } Cheers, } } Gary Gaugler, Ph.D. } } } } -------- REPLY, End of original message -------- }
I am about to do some estimates of our SEMs resolution under different conditions. I am intending on using gold particles of varying size for this task. I intend to analyse the change in intensity as the beam traverses a particle. I am worried about the subjective nature of such a test and would like to find a more objective procedure. Can anyone advise on other methods?
Question: How can serial sections be picked up onto a formvar/carbon coated large slot grid? So far I have had problems with wrinkling of sections, sections drying down onto metal instead of remaining in the slot window.
Message somehow bounced back. I am re-sending it. Sorry if you receive a duplicate. A few more words:
If you just want to mill one side of the sample on your PIPS, the other side does need to be protected from re-deposition with a 3mm thin disc of mica (you can use your disc cutter his time). Mica has numerous layers. Peel a thin piece from the top with a pair of good twizers. Overall, Preparing a plan view specimen of this kind of material should not be as difficult as preparing Chinese foods.
Regarding the handling of the prepared specimens, great care is of course the 1st thing I can mention. There are some other tricks. But please excuse me for not being capable of describing them in English. You will get some sense after a while. Also, if possible try a specimen holder with a specimen screw cap instead of a specimen clamp. Good luck. Dont get too much frustrated.
-cy
---------- Forwarded message ----------
POSTDOCTORAL POSITION IN INTERFACE PHYSICS
DEPARTMENT OF PHYSICS, UNIVERSITY OF ILLINOIS AT CHICAGO
{smaller} A postdoctoral research associate position is currently available in the Interface Physics Group at the University of Illinois at Chicago (UIC) to perform atomic scale analysis of interfaces and defects in semiconductor heterostructures. The successful candidate is expected to work closely with the MBE and microfabrication groups at UIC investigating a wide variety of technologically relevant systems (including II-VI, III-nitride, III-V, SiO/SiGeO, SiOxNy/SiGeOxNy and magnetic multilayers). =20
Research in the Interface Physics Group focuses on the use atomic resolution imaging and analytical techniques in electron microscopy, coupled with theoretical simulations, to determine the structure-property relationships at internal interfaces on the fundamental atomic scale. Current research programs involve ceramics, high-Tc superconductors and optoelectronic/high-power semiconducting materials and devices. The experimental facilities to perform this research are comprehensive: a JEOL 2010 Field-Emission STEM/TEM featuring a 1.4=C5 probe size, "drift free" stage, high-angle annular dark-field detector (Z-contrast), Gatan Imaging Filter, and Noran EDS; a VG HB501A Field-Emission dedicated STEM with EDS, EELS and Auger spectrometers; a JEOL 3010 conventional TEM with digital imaging capabilities and EDS; a JEOL 6320 Field-Emission SEM with EDS; and a JEOL JXA733 microprobe. In addition to the electron microscopes, specimen preparation facilities include a Gatan Duo-mill, Fischione precision ion-mill, SouthBay plasma cleaner and Leica Ultramicrotome.=20 The Interface Physics Group has a Silicon Graphics R10000 Power Indigo workstation with the Molecular Simulations' Cerius 2 package incorporating the CASTEP pseudopotential code. The physics department has additional workstations and access to the UIC Convex Exemplar Supercomputer and the National Center for Supercomputing Applications at UIUC. =20
Successful candidates will be recent Ph.D. graduates in physics, metallurgy, or materials science with a sound background in the relevant materials issues and an ambition to be part of a developing program pushing at the frontiers of interface physics. Please send a resume and publication list to Professor Nigel D. Browning at the address below. Prior experience in STEM or TEM is essential. =20 However, consideration will be based on the candidates overall potential for success in the field and applicants with prior experience in related fields are encouraged to apply. Positions are for one year initially, normally renewed for a second year with possibilities existing for further years. Salary is commensurate with experience.=20 UIC is an equal opportunity employer.
I have spoken with an executive from Mattel who said that the X3 microscopes will not be out on the market before the fall of 1999.
Carlton Bowers TECH TREK Mobile Research Laboratory (937) 222-2934
Subj: Re: Student Microscopes
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I have not been able to track down the Mattel microscpe yet. It is not on their website, the local Toys R Us store does not have it and the Mattel Merchandising Manager does not return my calls. I assume this is another example of publicity preceding product. I saw press releases in the San Francisco Examiner newspaper some time ago (?March) and last week in the new issue of Advanced Imaging. When I locate the actual product I'll post more information.
Larry D. Ackerman Lily & Yuh Nung Jan Laboratories Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Ave. San Francisco, CA 94143
by hms1.med.harvard.edu (Post.Office MTA v3.5.2 release 221 ID# 0-58538U3500L600S0V35) with ESMTP id edu; Fri, 16 Apr 1999 10:42:50 -0400
Dear Colleagues:
I have received quite a few inquires regarding to our posting about the used equipment available at Dana Farber Cancer Institute. The following is the information I could gather about those items:
I. The side entry TEM (JEOL 100 CXII) was purchased in 1988 ( purchase price $145,500). This is equipped with all standard features with additional specimen rotation and tilt correction device. The top entry scope was purchased a little earlier. The JSM-35 CF scanning electron microscope was purchased in 1982 ( purchase price of $75,900). All three scopes have been maintained under service contracts directly with JEOL. II. The Polaron sputter coater was purchased in 1983 (purchase price $6,180) with film thickness monitor, gold/palladium annular target, digital temperature indicator, and specimen holders.
I do not have information as to the exact time and price when the other items were purchased other than their model numbers. The Reichert microtome was purchased probably about 7-8 years ago. The MT5000 is probably 4-5 years older. As I said, they are all in excellent working condition.
As I understood from our administration, the institute would like to sell these equipment although it is also possible some of these items will be donated to non-profit organizations if no bids are received. Final decision will be made by our administration. Since I do not have pricing information, please feel free to make your reasonable offers and I will forward them to the institute. I will speak to our administration about possible arrangements for the demonstration of the equipment if necessary.
Thank you for your inquiries. Please feel free to email me if you have further questions. Best regards,
There is a press release with a brief description and a poor picture of the unit at: http://www.hottoynews.com/mattel1.htm Scott
} } I have not been able to track down the Mattel microscpe yet. It is not on } their website, the local Toys R Us store does not have it and the Mattel } Merchandising Manager does not return my calls. I assume this is another } example of publicity preceding product. I saw press releases in the San } Francisco Examiner newspaper some time ago (?March) and last week in the } new issue of Advanced Imaging. When I locate the actual product I'll post } more information. } }
-------------------note: new mailing address---------------------- Scott Wight e-mail: scott.wight-at-nist.gov NIST 222/A113 W voice: 301-975-3949 100 Bureau Dr STOP 8371 | fax: 301-417-1321 Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed is my own and does not represent those of my employer.
Dear Gary, I certainly beg to differ. I would buy a Hitachi S-570 over a JEOL 840 of the same age any day. I did when I evaluated them new in 1986. The S-570 is still running perfectly and still meets spec easily. I have two S-570s, one recently purchased used. All the old Hitachi's run forever. You wrote:
} } Actually, the brand and model of SEM has some degree of influence over } the depreciated value of the scope. For example: } Hitachi: 500, 520, 570 are boat anchors. } Hitachi: 800, 900 are FE scopes and are more modern. } Hitachi: 2960 and 3000+ are really more modern and can include the Nature option } and are very good scopes. } } } A JEOL 840 series is good. } } Philips? maybe a XL40FE. But as with any field emission scope, one } must be willing to put up with the extra grief of FE over LaB6. Yet, there } is an image intensity benefit. It is a tradeoff. } } gg } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I have made it a speciality of mine to study the performance of SEM all over the world. Running courses the first thing I need to know is how go= od is the instrument that I am using? For this you need a reliable standard=
that really tests the microscope. Any gold on carbon that I have used ha= s never been up to the task. It is quite possible to take photographs of this type of specimen and find that there is no difference in instrument performance from say 10 to 15kV, there must be a difference!
For this reason I developed a test specimen of my own which I have used f= or the past 18 years. The specimen is made up of polystyrene latex spheres that are allowed to dry from a liquid to form a solid white block. Durin= g the drying process, provided the latex preparation is free from contaminants, the spheres will deposit in an array that is of a square packing in one direction and hexagonal in the other. If a piece of the solid material is fractured, by pricking with a fine point, this opens up=
the internal structure of the compacted material, displaying the two type= s of array.
Having a very well defined structure the hexagonal arrays make a very goo= d subject for judging the performance of a scanning electron microscope. A= ny hexagonal area on the specimen is comparable with another set in the same=
orientation. Another advantage of this specimen is that the latex are of= a specific size which may act as an inbuilt calibration. In most cases of performance monitoring the operator simply needs to take a test picture a= t a specific magnification and use a comparative process to judge performance. The latex are a nominal 0.24um but when compacted in an arr= ay they are visibly reduced to about 0.2um.
Should the sample become damaged it is easily recovered by re coating or once again pricking it with a pin to open up new areas and then re coatin= g.
Making A Test Specimen
To convert the latex specimen into a high resolution test specimen a meta= l coating is required. A sputter coating will make the specimen conducting=
but further coats will build a sub structure on the surface of the sphere= s. The sub structure may be used for high resolution performance monitoring= . =
The level of sub structure desired will depend upon the capabilities of t= he instruments to be investigated. For instruments with a conventional tungsten source multi coating the latex with gold is satisfactory. For more advanced instruments the finer coating of gold-palladium may be more=
desirable.
The coating procedure depends upon the efficiency of the coater being use= d. Sputter coaters that use relatively high voltages (1 to 3kV) will requir= e the following procedure.
i. Set the coater at a 5cm target to specimen distance. ii. Sputter at 20mA, 1kV for one minute, wait one minute and repeat t= he process. iii. Coat for 4 one-minute periods and then check the specimen in the microscope. iv. If you need more coats, because you cannot see the metal, repeat the "coat and wait" procedure until the structure is satisfactory.
The more metal you put down the coarser the structure will be on the spheres. Low levels of coat will require better operating techniques in order to resolve the coat. Do not expect a conventional (W hairpin) instrument to be able to resolve less than 4 coats.
If you have a modern coating unit, which will be much more efficient at putting down the coat, use 10mA for 30 seconds per coat. Experimenting with coating procedures will enable you to tune the coating parameters an= d coating time to obtain the exact specimen that you require. For field emission instruments a gold-palladium coat, if carefully applied, will gi= ve you grains in the region of 8nm and a spacing of less than 1nm.
Operating Procedures
If you intend to push yourself and your microscope to near its limits the= re are some basic operating procedures that will be required. Firstly the specimen must be placed in the instrument and the high voltage must be switched on for at least 45 minutes prior to trying to work at high magnifications (} 30,000X). This period is required for the high voltage tank, and hence the high voltage, to reach stability. After this period the heat gained by the components is equal to the heat lost through the walls of the tank and the high voltage will be at its most stable.
Whilst stability is being achieved move the specimen to a short working distance ( {5mm) and set the instruments alignment to the best of your ability. Find areas of the specimen that are in the hexagonal array and flat to your view. A slight tilt of the array is not as good for comparison as is a perfectly flat surface; you may need to tune your specimen tilt slightly.
Hope this helps make your own and try it?
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
Chris asks ... } } } I am about to do some estimates of our SEMs resolution under } different conditions. I am intending on using gold particles } of varying size for this task. I intend to analyse the change } in intensity as the beam traverses a particle. I am worried } about the subjective nature of such a test and would like to } find a more objective procedure. Can anyone advise on other } methods? } } Chris Walker
The best method, or least subjective, would be to do a line scan perpendicular to a knife edge. This allows a measurement of signal intensity a function of distance, and you'd be able to quantifiably compare your different parameters by comparing the distance between two points ... e.g., at 20% maximum and 80% maximum.
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I have two older SEM/EDS systems for sale. Where do I advertise? How do I get the word out most effectively? Any suggestions welcome. Is there a Web site where one can post SEM's for sale?
Dear Listers, For those who may be interested in upgrading an existing EDX system to a Windows NT, PC-based system, check out the Quartz Imaging web site for their lastest announcement: www.quartzimaging.com. I've tried a demo system and it's excellent.
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I would once again like to tap the collective expertise (and my apologies for not thanking each of you each time you help me out)!
A researcher is trying to fix isolated mitochondria (rat brain, I think) that have been subjected to different treatments just before isolation. He expects to see different degrees of swelling. Being extremely conservative about data, I need to make sure the swelling we see is not due to osmotic stress, among other artifacts. Does anyone have a favorite recipe for isolated mitochondria? So far 2.5% glut in 0.1M cacodylate, postfixation with 1% OsO4 in cacodylate, and en bloc uranyl acetate in the 50% ethanol dehydration step seems adequate, but not great.
Thank you all in advance!
Aloha, Tina
No surf today. **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
If I understand you correctly, you want to measure the resolution of the image, i.e., the finest detail that can be resolved with the microscope, not its reproducibility or linearity.
Perhaps you can take a clue from the way this is done on TEMs:
On a TEM you acquire an image of an amorphous material. The Fourier Transform of the image is then either calculated or produced by using an optical diffractometer. This FT then shows the frequency distribution of the image. At the center you have the low frequency information, i.e., the slowly varying brighness changes, and the further out you go, the higher the frequencies. In a TEM, this information is modulated with the contrast transfer function, leading to rings of intensity. By including a little bit of crystalline material with a known lattice constant, this FT image can be calibrated and the radius determined, at which the information (or intensity in the FT image) disappears. This radius is the inversly proportional to the smallest distance that can be resolved.
For an SEM you will need a sample that shows structure or features around the expected resolution limit. Then take an image and calculate the FT. This is best done on a computer. Make sure, that the pixel size is at least a factor of two smaller than the expected resolution or you will see artifacts from pixelation.
The images from the SEM will not show the rings from the CTF, but by doing a radial integration of the FFT you should be able to determine the point, where the intensity in the FFT has gone down below a threshold. You can then use this graph as a measure of your resolution.
Good luck.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com
} ---------- } From: Chris Walker[SMTP:chris.walker-at-physics.org] } Sent: Friday, April 16, 1999 1:30 PM } To: 'Microscopy-at-MSA.Microscopy.Com' } Subject: SEM: Resolution tests } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } I am about to do some estimates of our SEMs resolution under different } } conditions. I am intending on using gold particles of varying size for } this } task. I intend to analyse the change in intensity as the beam } traverses a } particle. I am worried about the subjective nature of such a test and } would } like to find a more objective procedure. Can anyone advise on other } methods? } } Chris Walker }
Dear Chris, The best way to test resolution is to take your best picture at each operating condition and see how small a feature you can resolve. That is the definition of resolution. I actually examine the Polaroid negative with a 10X magnifyer and measure the smallest gap between gold particles on a carbon substrate that I can see (at about 50,000 or 100,000X mag.). Divide by the mag to get the resolution in nm. You need a sample with a variety of gold particle sizes on it. You wrote:
} } I am about to do some estimates of our SEMs resolution under different } conditions. I am intending on using gold particles of varying size for this } task. I intend to analyse the change in intensity as the beam traverses a } particle. I am worried about the subjective nature of such a test and would } like to find a more objective procedure. Can anyone advise on other } methods? } } Chris Walker } Best of luck, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Hi Sheila R. I happen to publish Microscopy Today, a publication which is sent 10 times a year to over 8,400 microscopists in the U.S. - each of which who has requested a no cost subscription. I do have an used equipment section - charging a modest $25 plus a dollar a word, with a $50 minimum. I also publish employment opportunities - also with modest rates. Kindly advise by return email should you be interested in this service. Others might comment to you on any value they may feel with my pub. Best, Don Grimes, Microscopy Today
Dear All: I have a question on the problem of Kevex EDS system which I have here since November, 1997. The system is with a thin window detector and attached to the Topcon SEM. The problem is that sometimes the peak position in a spectrum is found shifted around 300 eV downside from the right position. It happens anytime, even after calibration, or after acquiring data from standard specimen for the qualitative or quantitative analysis. The higher the energy is, the larger the amount of shifting is. The peak width stays thin but sometimes become broad. When I change the bias voltage of the detector from 500 to 450 or 400 V, the shifting is getting worse or better, without specific direction. Service man from the Kevex has been trying to solve the problem for seven or eight months, but still they could not find the source of problem. It could be FET in the detector, preamp, analyzer, cable, plug, or whatever. The most difficult thing is that the shifting problem does not happen all the time. It disappears all of a sudden and does not occur for a few days, and shows up again, driving me and service men crazy. Does any of you have experienced this kind of shifting problem? Could you figure out the cause of the problem? It is really a nuisance and I I want to get rid of it ASAP. Any information will be appreciated. Sincerely, Jondo Yun, Ph.D.Department of Inorganic Materials Engineering Electron Microscopy LaborotaryCenter for Instrumental AnalysisKyungnam University449 Weolyeong-dong, Masan, 631-701, Korea82-551-249-2697 (tel)82-551-248-5033 (fax)jdyun-at-hanma.kyungnam.ac.kr
It looks like something in the feedback loop is flaky. The fact that changing the detector bias sometimes affects the shift makes it look like something in the front end. The most obvious cause would be something wrong with the feedback capacitor in the JFET package, maybe a dust fiber flipping back and forth under the influence of charging. The feedback capacitor is on the order of 0.05 picofarads, so it wouldn't take much of a piece of lint to change the gain by 300 eV.
best regards mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
Jundo wrote:
Dear All: I have a question on the problem of Kevex EDS system which I have here since November, 1997. The system is with a thin window detector and attached to the Topcon SEM. The problem is that sometimes the peak position in a spectrum is found shifted around 300 eV downside from the right position. It happens anytime, even after calibration, or after acquiring data from standard specimen for the qualitative or quantitative analysis. The higher the energy is, the larger the amount of shifting is. The peak width stays thin but sometimes become broad. When I change the bias voltage of the detector from 500 to 450 or 400 V, the shifting is getting worse or better, without specific direction. Service man from the Kevex has been trying to solve the problem for seven or eight months, but still they could not find the source of problem. It could be FET in the detector, preamp, analyzer, cable, plug, or whatever. The most difficult thing is that the shifting problem does not happen all the time. It disappears all of a sudden and does not occur for a few days, and shows up again, driving me and service men crazy. Does any of you have experienced this kind of shifting problem? Could you figure out the cause of the problem? It is really a nuisance and I I want to get rid of it ASAP. Any information will be appreciated. Sincerely, Jondo Yun, Ph.D.Department of Inorganic Materials Engineering Electron Microscopy LaborotaryCenter for Instrumental AnalysisKyungnam University449 Weolyeong-dong, Masan, 631-701, Korea82-551-249-2697 (tel)82-551-248-5033 (fax)jdyun-at-hanma.kyungnam.ac.kr
Mattel had made an error in my email address which delayed the following press release:
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Bringing the microscopic world to life, the QX3 Computer Microscope allows children to view and manipulate everyday things with a fun, magnified perspective. Embarking on a voyage of discovery, children can use the microscope on the base to examine a prepared slide or anything else they have collected magnifying it on the computer. Children can also use the detachable hand-held viewer to look at an image such as ants, moldy cheese or even view the freckles on their own face! Children can let their imaginations run wild by combining and manipulating images in fun and wacky ways using colorizations and special effects. The QX3 Computer Microscope web site includes an on-screen reference guide which helps kids identify and analyze the bugs they've found.
Proving that creativity knows no limits, the QX3 Computer Microscope empowers children to save the images as a still photo, short video clips or time-lapse movies. The ultimate creative self-expression is making "shows" by sequencing and editing the captured images, adding music and sound effects. Children can also print posters, stickers and more. The=20 QX3 Computer Microscope includes the microscope and CD-ROM, as well as slides and specimen holders, plastic tweezers, an eye dropper, brine shrimp, brine shrimp food and user documents. # # #
**Microsoft and Windows are either registered trademarks or trademarks of Microsoft Corporation in the U.S and/or other countries ***Intel is a registered trademark and Intel Play is a trademark of Intel Corporation
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Hi, I usually don't prepare the slot grids with the formvar/carbon film in the hole before picking up sections. Instead, I precoat the slot grids with formvar - making them somewhat hydrophobic - and then pick up the sections. The sections float in the hole. They are then laid down on formvar in holes of a "bridge" to dry down. The bridge is a bent aluminum structure that has holes drilled in it that a sheet of formvar is laid over. One collects the formvar floating on water with the bridge rather than laying grids on it. Once dry, the grids with sections can then be laid on the formvar suspended in the bridge holes, and carefully removed later (without tearing the formvar on the grid - takes some practice).
The bridges can be made, or ordered from one of the usual EM vendors.
On Fri, 16 Apr 1999 08:19:22 -0600 ckuzmiak-at-chuma.cas.usf.edu wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Colleagues ..... } } This one is out of my area of expertise... Please reply direct to } the sender... } } Email: ckuzmiak-at-chuma.cas.usf.edu } Name: Carolyn Kuzmiak } School: USF } } Question: How can serial sections be picked up onto a formvar/carbon } coated large slot grid? So far I have had problems with wrinkling of } sections, sections drying down onto metal instead of remaining in the } slot window. } } --------------------------------------------------------------------------- } }
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
Dear sir i am Dr s.k. jatty associated with the r&d division of the cement group of The A.C.C. ltd, mumbai, india working on the field of cement and concrete microscopy.
I would like to have some information about the laboratories and institutios all obver world working inthis particular field. Hope u will reply positively.
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Organized by McCann Imaging For further information: see web page at www.microscopyed.com For course brochure, contact Mary McCann, McCann Imaging 161 Claflin Street Belmont MA 02478 e-mail: mccanns-at-tiac.net Phone (617)-484-7865
Tina Carvalho wrote: } } I would once again like to tap the collective expertise (and my apologies } for not thanking each of you each time you help me out)! } } A researcher is trying to fix isolated mitochondria (rat brain, I think) } that have been subjected to different treatments just before isolation. } He expects to see different degrees of swelling. Being extremely } conservative about data, I need to make sure the swelling we see is not } due to osmotic stress, among other artifacts. Does anyone have a favorite } recipe for isolated mitochondria? So far 2.5% glut in 0.1M cacodylate, } postfixation with 1% OsO4 in cacodylate, and en bloc uranyl acetate in the } 50% ethanol dehydration step seems adequate, but not great. } Dear Tina, We have had good luck with high-pressure freezing. We have examined unstained, frozen-hydrated mitos on the 400 kV instrument here. Although this procedure requires instruments not available at all facilities, I expect to get minimal artefact. Since we are a NIH resource, investigators can use our facilities for free, so if your colleague wants to try this, but doesn't have access to the requisite equipment, (s)he can apply to use our resource by accessing our web site, www.wadsworth.org/bmirr Yours, Bill Tivol
In a message dated 4/19/99 6:10:52 AM Hawaiian Standard Time, { { } Email: ckuzmiak-at-chuma.cas.usf.edu } Name: Carolyn Kuzmiak } School: USF, writes: } } Question: How can serial sections be picked up onto a formvar/carbon } coated large slot grid? So far I have had problems with wrinkling of } sections, sections drying down onto metal instead of remaining in the } slot window. } }
A technique I have used is to pick the secions out of the water trough using a thin loop (I made mine of .008 gold/paladium wire for no better reason than that is what I had in the lab!). The loop should have a diameter of less than 3mm. 2mm works well.
If the section ribbon is teased away from the knife edge the loop can be lowered from the top and will pick up a "film" of water plus sections.
A slot grid, with a formvar/carbon support film is used. The grid needs to be held firmly during the transfer process of the secions from the loop. I actually use an old Hitachi specimen holder where the grid is secured on top of a 3mm dia peg with a retaining cap. This kind of setup can easily be made in the workshop. The grid can also be held by the adhesive applied to "gridsticks" (available from EM supply companies).
The loop is lowered onto the filmed surface of the grid and the water drop with sections transfers readily to the support film.
Treating the filmed grids with poly-L-lysine beforehand also helps. ( Apply a drop of .01% poly-L-lysine to the filmes surface of the grid. After 5 mins blot off with filter paper applied to edge of grid. Allow grid to dry at room temperature or more quickly in an oven at 60 degrees centigrade.)
Does anybody have ever used BALTEC RES 100 for TEM (or SEM) specimen preparation? any comment ? I am carrying out a comparison with PIPS (Gatan) and LAMP 1010 (Fischione) in order to select the equipement to be purchased by my institute. We need it to prepare inorganic samples for TEM observations, mainly Materials Science research activities.
Any kind of information will be very useful. Thank You all in advance, Edoardo Bemporad
Does anybody have ever used BALTEC RES 100 for TEM (or SEM) specimen preparation? any comment ? I am carrying out a comparison with PIPS (Gatan) and LAMP 1010 (Fischione) in order to select the equipement to be purchased by my institute. We need it to prepare inorganic samples for TEM observations, mainly Materials Science research activities.
Any kind of information will be very useful. Thank You all in advance, Edoardo Bemporad
-----Original Message----- } From: THE A.C.C. - RCD - THANE [mailto:rd3-at-bom3.vsnl.net.in] Sent: Monday, April 19, 1999 4:34 PM To: Microscopy-at-Sparc5.Microscopy.Com
Dear sir i am Dr s.k. jatty associated with the r&d division of the cement group of The A.C.C. ltd, mumbai, india working on the field of cement and concrete microscopy.
I would like to have some information about the laboratories and institutios all obver world working inthis particular field. Hope u will reply positively.
kindly mail me " jattysk-at-hotmail.com"
thanking you with regards
Dr s.k.jatty
Dr. Jatty,
I suggest contacting the International Cement Microscopy Association, 1206 Coventry Lane, Duncanville, TX 75137, USA. Membership is free, and meetings are held in the spring once a year at a North American city. This organization covers all aspects of cement and concrete microscopy.
George Polkowski Lab R&D Center Saudi Aramco Dhahran, Saudi Arabia
1. Measuring resolution by measuring the wave-form across an edge is=
not very constructive in the real world. Designed to monitor spot size,=
with the assumption that the resolution attainable is similar to the smallest spot, this technique is messy and in no way does it develop in a= n operator for the correct procedures to attain maximum performance.
Visual indications of performance or lack of it are very important when operating at the limits of the instrument at a particular kV. Using "normal" images for performance testing helps the operator develop a feel=
for the instrument, thereby sensing that they could use a smaller spot size, or a higher emission current, a better astigmatism correction or better focus. Not only do you test the instrument but you also test and develop the operator. Reasons why the South African Microscopical Societ= y are looking at a basic "quality" procedure for EM nation wide.
2. The TEM method of using an optical diffractometer is very interesting. I have used it many times for TEM and it would work for SEM=
negatives, one problem is that the biggest users of SEM are in industry a= nd few will have access to a diffractometer.
Just a few thoughts on an interesting but to many rarely discussed question, or is it?
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Does anybody have ever used BALTEC RES 100 for TEM (or SEM) specimen } preparation? any comment ? I am carrying out a comparison with PIPS (Gatan) } and LAMP 1010 (Fischione) in order to select the equipement to be purchased } by my institute. We need it to prepare inorganic samples for TEM } observations, mainly Materials Science research activities. } } Any kind of information will be very useful. Thank You all in advance, } Edoardo Bemporad
Dear Edoardo Bemporad,
we use the BAL-TEC RES 100 about one year and mainly for TEM sample preparation of semiconductor heterostructures (SiC/Si, AlN/Si, ...). But I have no experiences with the Gatan and the Fischione equipment. What kind of information are useful for you about the RES 100?
Dr. Jatty, The International Cement Microscopy Association has a web site at http://www.cemmicro.org/ Greg
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
Our lab is removing the basement membrane from sheets of tissue containing Bruch's membrane. We want to verify that the basement membrane has been removed. We would like to do this by labeling pieces for the presence of basement membrane. Positive label would indicate unsuccessful removal. Is there a specific label for basement membrane that would not label underlying material such as the collagen of Bruch's membrane?
I have following recipe to mix the four components for EPON 812. For 25grs we use 11.556 g Epikot 7.125 g Dodecanylsuccinateanhydride, 6.275 g Methylnodicanhydride and 0.375 g DMP-30 (2,4,6 Triphenol)
Well, anyone who uses other formulas to mix Epon or do you agree with my
formula? I would be happy to receive other propositions to mix Epon or any further information on this stuff. It is the first time I try Epon while using the 3-component ARALDITE normally.
I am looking to buy a used EDX specimen holder for a JEOL 1200EX or EXII. If you have one that you haven't used and are willing to part with, please e-mail me at: jfb-at-uidaho.edu
On Mon, 19 Apr 1999 DUNNTEM-at-aol.com-at-Sparc5.Microscopy.Com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } In a message dated 4/19/99 6:10:52 AM Hawaiian Standard Time, { { } } Email: ckuzmiak-at-chuma.cas.usf.edu } } Name: Carolyn Kuzmiak } } School: USF, writes: } } } } Question: How can serial sections be picked up onto a formvar/carbon } } coated large slot grid? So far I have had problems with wrinkling of } } sections, sections drying down onto metal instead of remaining in the } } slot window. } } } } A technique I have used is to pick the secions out of the water trough using } a thin loop (I made mine of .008 gold/paladium wire for no better reason than } that is what I had in the lab!). The loop should have a diameter of less than } 3mm. 2mm works well. } } If the section ribbon is teased away from the knife edge the loop can be } lowered from the top and will pick up a "film" of water plus sections. } } A slot grid, with a formvar/carbon support film is used. The grid needs to be } held firmly during the transfer process of the secions from the loop. I } actually use an old Hitachi specimen holder where the grid is secured on top } of a 3mm dia peg with a retaining cap. This kind of setup can easily be made } in the workshop. The grid can also be held by the adhesive applied to } "gridsticks" (available from EM supply companies). } } The loop is lowered onto the filmed surface of the grid and the water drop } with sections transfers readily to the support film. } } Treating the filmed grids with poly-L-lysine beforehand also helps. ( Apply a } drop of .01% poly-L-lysine to the filmes surface of the grid. After 5 mins } blot off with filter paper applied to edge of grid. Allow grid to dry at room } temperature or more quickly in an oven at 60 degrees centigrade.) } } Hope this helps. } } Ted Dunn } Maui, Hawaii } Hi,
Very good ideas! I do it mostly the same way, but I don't use a bridge. It also works if you do not coat the "pick-up grid" with formvar. In order to hold the final, filmed- with- formvar grid steady when transferring the section from the water drop, the grid can be clamped into a reverse forceps (I have 5 of them). The grids in the five forceps can be laid down or put into a holder after the water is drawn off with a filter paper (or not - the grids can just be allowed to dry). One has to try it out, because depending on the embedment and the specimen one method may be more liable to cause wrinkling than another. Another very helpful idea is to use boat water which has been treated by sitting in one of the Pella cups meant for this purpose. Using this water prevents the sections to pedal around in the boat and mixed up. If the sections are irreplaceable, etc and are due to make you rich and famous, then another method may be in order. Trim a HUGE block face. Section one section at a time, and pick up each section seperately on a seperate grid. Tedious, but won't we do to become rich and famous? Bye, Hildy Crowley {hcrowley-at-du.edu}
Our laboratory is planning to buy a print processor. Can anyone give us a recommendation? We would like something simple like the ancient Ilford type which is fast and easy. We can fix prints at a later date seperately. I would so much appreciate anyone's comments with their hands-on experience.
I agree that it would be very unusual for an SEM site to have an optical bench for optical diffractometry. But as I mentioned in my posting, this should also work by acquiring the image on a computer (everybody has digital image acquisition on the SEM, right? If not, contact us. So much for a plug. See disclaimer below) and calculating the Fourier Transform of the image. Then you have to do the radial integration and you should be able to judge the resolution. Using optical diffraction is better as it includes more information from a negative, so for doing it on a computer, you want an image as big as possible (min. 2K x 2K). Whether this also works with images scanned from Polaroids I don't know.
I hope, this helps.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com
******************************************************* Disclaimer: Soft Imaging System produces and sells image acquisition and processing systems. We therefore have a vested interest in some of the items mentioned above. *******************************************************
} ---------- } From: Steve Chapman[SMTP:PROTRAIN-at-CompuServe.COM] } Sent: Tuesday, April 20, 1999 12:55 AM } To: American } Subject: SEM Resolution } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } The few comments posted pose interesting points. } } 1. Measuring resolution by measuring the wave-form across an edge } is } not very constructive in the real world. Designed to monitor spot } size, } with the assumption that the resolution attainable is similar to the } smallest spot, this technique is messy and in no way does it develop } in an } operator for the correct procedures to attain maximum performance. } } Visual indications of performance or lack of it are very important } when } operating at the limits of the instrument at a particular kV. Using } "normal" images for performance testing helps the operator develop a } feel } for the instrument, thereby sensing that they could use a smaller } spot } size, or a higher emission current, a better astigmatism correction or } better focus. Not only do you test the instrument but you also test } and } develop the operator. Reasons why the South African Microscopical } Society } are looking at a basic "quality" procedure for EM nation wide. } } 2. The TEM method of using an optical diffractometer is very } interesting. I have used it many times for TEM and it would work for } SEM } negatives, one problem is that the biggest users of SEM are in } industry and } few will have access to a diffractometer. } } Just a few thoughts on an interesting but to many rarely discussed } question, or is it? } } Steve Chapman } } Senior Consultant E.M. } Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. } Tel & Fax 44 (0)1844 353161 } Web Site - http://ourworld.compuserve.com/homepages/protrain } For Consultancy and Courses in Electron Microscopy World Wide } }
Senior Research Technician*- Oklahoma Medical Research Foundation = Imaging Facility =20 The newly formed Imaging Core Facility at the Oklahoma Medical Research = Foundation is seeking a research technician with a BS/BA degree in life = sciences (minimum) and a background in preparatory methods for light = and/or electron microscopy. Candidates should be able to carry out = standard specimen fixation, embedding, sectioning and staining = techniques. Prior experience in mammalian tissue processing and some = knowledge of histological methods would be helpful but are not required. = Salary will be commensurate with experience. The successful applicant = will receive further training on the TEM, laser scanning confocal = microscope and other instruments. Please submit a Curriculum Vitae and = names and addresses of three references to:
Stephen Fields, Ph.D. Oklahoma Medical Research Foundation Program in Molecular and Cell Biology 825 NE 13th St. Oklahoma City, OK 73104 405-271-7245 (office) 405-271-3153 (fax) steve-fields-at-omrf.ouhsc.edu
*Applicants must be capable of a full range of body positions and = movements including, but not limited to, standing, walking, sitting, = climbing, bending/stooping, squatting, twisting, and reaching. Moreover, = fine motor skills and sense of touch are necessary to carry out the many = procedures associated with the job, some of which could be dangerous = without full or nearly full motor and sensory attributes, including = hearing and vision.=20
I am looking into options for visible microspectrophotometry, and would appreciate your suggestions. A system could be added to one of our microscopes (Olympus BX60 or BH2), or adapted to our Nicolet FT-IR spectrometer/Spectra-Tech microscope.
Recommendations from satisfied users of either option, or another, will be appreciated.
The following are comments that I gave to Edoardo Bemporad with several items added. It thought that it might be useful to this crowd since there is not a lot of experience out there with the RES100 ion mill.
I assume that you would like my recommendations with respect to the Bal-Tec ion mill. I like it very much. I am getting very good samples. There are things that the RES 100 can do that the other ion mills can not. In particular, the RES100 can coat non-conducting samples with a sputter coating quite easily and can also etch samples. These last two things become very important if you have a reasonably good SEM. I have also done a little ion polishing of SEM samples that were examined in a high resolution SEM with good results.
The capability of imaging the sample for alignment is very significant for me because most of my samples are cross sections of glass samples and they fluoresce under the mill. With every sample, I can routinely check the ion mill alignment if I want to. The RES100 has the ability to capture the digital image of the fluorescence and superimpose it on the image. This allows you to see when shadowing of the center of the sample occurs due to the rim of the dimple. This then defines the minimum milling angle that you can use.
I have found that the alignment of the guns are extremely good over time. I can say that I have not had the same experience with the limited use that I have had with a PIPS system. I use the RES100 to sputter coat carbon onto my glass samples so that I can use them in a FEG-TEM. (If I coat them with my normal evap carbon coater, they contaminate because it is a dirty diffusion pumped system.) It could be set up to sputter coat other materials quite easily.
There are areas for improvement in the mill and Bal-Tec is very receptive to good suggestions. They have made a number of corrections or improvements suggested by me.
The low-angle stage works well, but I have dropped some samples while milling. I have found that this is less of a problem if I am careful and make sure that my samples are less than 100 um in total thickness. (Around 90 um works very well.) Bal-Tec is aware of this and as I understand it are looking at alternative designs for the low angle stage. We also had some problems with the rotation motor early on, but they did a redesign of the motor mount that fixed the problem and have had no problems since.
I bought the RES100 because at the time, it was only one of two instruments that had the ability to terminate with a Faraday cup. This feature works well. However, I find that the image processing termination feature of the RES100 is much more sensitive and fine tunable than the PIPS. Again, they made a few modifications to this portion of the software after some comments by me and others that significantly improved it. It would be able to set up silicon, for example, when it becomes optically transparent in the red. It looks like it is very possible to use image processing to terminate optically transparent samples. I showed them some image processing steps that could do this, but I don't know if they will implement it or not. I thought that they were interested in this.
Programming the ion milling is fairly straightforward. I would like to the ion milling history that a sample goes through recorded better. It is also not easy to perform milling from the substrate side on both sides of the sample like the PIPS instrument does. It is doable, but only with one gun at a time. The best way to do it is by cycling the same gun on top and bottom of the sample and rocking the sample back and forth (without rotation) with an angle of 30-35. Since you can't yet loop the program, you have to brute force it by copying two lines of the program and pasting them many times.
There is a learning curve to the use of the instrument that is a little longer than the other ion mills that I have used. I think that this is so because the design and philosophy of the instrument is different than others.
I have used the PIPS instrument and am familiar with the Fischione instrument.
Given the choice between the three instruments, I would buy the RES100 again if I had to make the decision tomorrow because of its versatility. However, I understand that the ion mill South Bay Technology sells (IV-3?) now has the Faraday termination option and I would like to check that and compare it to the RES100.
Overall, I am quite pleased with my system and the service that I received from Bal-Tec.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P.O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
BookWhere? is a powerful software package that runs on the Microsoft Windows Operating System and allows the user to search remote databases for published material of interest.
Link to this site is available on www.coleoptera.org in the directory {Software house} .
Hundreds of library catalogues and other types of information databases can be searched via the Internet using BookWhere? The software comes with a pre-configured list of available hosts and databases making access to this universe of information easy and convenient.
The Essential Research Tool
SINGLE INTERFACE: This single interface is your window on global libraries. BROADCAST SEARCHING: Fast, direct, simultaneous access to remote databases. COMPREHENSIVE SOURCES: Select library catalogues from built in lists organized by name, location or type. HYPERLINK SEARCHES: Hyperlink to a second level search directly from retrieved records. ANALYZE RESULTS: Customize the analysis window for grouping records by various keys. Jump to a subset instantly. EASY TO USE: Easy-to-use Windows-based interface. EXPORT RECORDS: Supports ALL popular bibliographic citation management packages. Transfer records directly to ProCite and Reference Manager. MARC RECORDS: Supports the display and export of MARC records for use in library automation systems
link to this site is available on www.coleoptera.org directory {Software house} .
Keep care and be of good cheer.
Regards
(name) Vratislav Richard Eugene Maria John Baptiste (surname) of Bejsak (Bayshark)-Collorado-Mansfeld
http://www.coleoptera.org Coleoptera - Australia, Tenebrionidae of World (incl. Lagriinae, Alleculinae)
University of Sydney The Wentworth Bldg., P.O.Box 62 NSW 2006 AUSTRALIA phone : +61 414 540 465 e-mail: vratislav-at-bigfoot.com ricardo-at-ans.com.au (before Ricardo-at-compuserve.com and ricardo-at-login.cz )
Only after the last tree has been cut down, only after the last river has been poisoned, only after the last fish has been caught, only then will you find that money can not be eaten.' CREE INDIAN PROPHECY.
Michael Reiner wrote I have following recipe to mix the four components for EPON 812. For 25grs we use 11.556 g Epikot 7.125 g Dodecanylsuccinateanhydride, 6.275 g Methylnodicanhydride and 0.375 g DMP-30 (2,4,6 Triphenol)
Well, anyone who uses other formulas to mix Epon or do you agree with my
formula? I would be happy to receive other propositions to mix Epon or any further information on this stuff. It is the first time I try Epon while using the 3-component ARALDITE normally.
Dear Michael Reiner,
for Epon 812 with WPE 157 we use
52,3 g Epon 23,0 g MNA 23,0 DDSA 1,5g DMP30 or 2,5 g BDMA
best wishes Anne Heller
Dr. Anne Heller Arbeitsgruppe Elektronenmikroskopie Institut fuer Botanik (210) Universitaet Hohenheim Garbenstr.30 D-70593 Stuttgart Tel.0049-711-459-2180 Fax 0049-711-459-3355
We calculate for each bottle of Epon 812(or EMBed 812). Most commercially available epoxy resins sold today give the "Weight for Epoxy Equivalent" of the Epon component. The "WPE" is listed on the bottle for each batch you buy. This value for Epon 812 usually varies between 140-180. If the same resin consistency is to be maintained, formulation of the resin must change to compensate for the varying WPE's of the resin. This can be done by varying the amount of hardener(anhydride) For example, the weight of the anhydride used can be determined from the following formula:
weight of anhydride= (weight of resin/WPE) x anhydride equivalent x anhydride/epoxy ratio
WPE is determined from label on bottle. anhydride equivalent = molecular weight of DDSA (Dodecenyl Succinic Anhydride) = 226.0 NMA (Nadic Methyl Anhydride)=178
Ratio of anydride/epoxy = ratio of molar concentrations of anhydride to epoxy Luft's original 1961 mixture that gave optimal cutting qualities for Epoxy 812 (also known as Epon or EMBed 812) was 0.7 A/E.
Example: If you wish to use 100 grams of Epon resin with a WPE of 160, how much DDSA and NMA would you need to achieve an A/E of 0.7?
DDSA weight = (100/160) x 266 x 0.7 = 116.4 grams NMA weight = (100/160) x 178 x 0.7 = 77.87 grams
You would combine 100 grams of Epon with 116.4 grams of DDSA and then 77.87 grams NMA with 100 grams of Epon. (note that you end up with 200 grams of Epon at the end) Mix these well and add DMP-30 at a 1-3% ratio (we usually use 1%) And of course, we cut the amount to fit in a 30 ml syringe for storage and ease of use.
Good luck. john
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
On Tue, 20 Apr 1999 16:32:52 +0200 Michael Reiner {a2811111-at-smail.Uni-Koeln.DE} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear microscopists: } } I have following recipe to mix the four components for EPON 812. } For 25grs we use } 11.556 g Epikot } 7.125 g Dodecanylsuccinateanhydride, } 6.275 g Methylnodicanhydride } and 0.375 g DMP-30 (2,4,6 Triphenol) } } Well, anyone who uses other formulas to mix Epon or do you agree with my } } formula? } I would be happy to receive other propositions to mix Epon or any } further information on this stuff. } It is the first time I try Epon while using the 3-component ARALDITE } normally. } } Thank you for hints and tips, } keep trying ... } } Michael Reiner }
Sorry, I forgot to add the Epon/araldite info. If you are using an Epon/araldite formulation, go by the amounts listed in the kit. If you lost this, you can contact any of the suppliers and they are usually kind enough to mail that info. I tend to stick with my "usual and customary" amount for this (as I am a naturally lazy person):
Embed 812: 25 grams or 25 ml Araldite 502: 16 grams or 15 ml DDSA: 48 grams or 55 ml DMP 30: 1.6 gram or 1.5-2 ml
I'm sure that there are others that have a more precise or different rendering of this formula.
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
Hi all! I could do with a bit of serious technical advice from someone with experience in preparing diffraction crystals. Basically, it goes like this. I've pillaged the diffracting crystals from an old Phillips spectrometer that we have sitting in the basement. Inside were LiF, LiF220, Ge, PET and TAP on their nice Al backing plates attached with dollops of silicon (?) rubber sealant. Because of the exorbitant price of replacing diffraction crystals for our JEOL 'probe, particularly TAP at ca. R40,000 each, I thought that it might be a cunning ploy to remove these from their backings and cut them to size. I could get several and if they work that would be great. Not only would it sort out our TAP problem but also extend our choice of crystals to use. I have located a diamond wire saw with which I could do the cutting. However, there are certain other things that spring to mind for which help from those who actually prepare these things would be greatly appreciated. I appreciate that those who produce these for the JEOL 'probes would rather I bought new ones from them. Sorry guys, but our lab can't afford it with the current state of the Rand. 1) Is the diamond saw good enough for this kind of work? 2) TAP appears particularly fragile, is there a way to minimise flaking? 3) I guess lubricant should be avoided, but if it is possible to use one, what should be used for which crystal type?
Any help would be greatly appreciated. Cheers, Malc. Dr MP Roberts Department of Geology Rhodes University Grahamstown 6140 South Africa Tel: +27 46 6038316 Fax: +27 46 6229715 ******************************* "If God had meant birds to fly, he would have given them engines" Anon.
For those of you who have not yet used microspectrophotometry, it is a great way to add optical and chemical information to our microscopy imaging. Spectroscopy and Microscopy are converging technologies; that convergence was strongly visible at this year's PITTCON meeting. (For details, write me or see the May issue of American Lab, "Focus on Microscopy" column)
But back to Jamie's questions: Leica and Zeiss both make fully integrated UV-Vis microspectrophotometers, with the necessary software for spectral manipulation. In terms of add-ons, there are now 2-3 companies, but I would have to do research to dig them out. The only one I have current data on is a company run by Felix Brogna called Optical Technologies. They are located in Hawthorne, NY (just north of White Plains) and can be reached at 914-592-1900. While I know that there are at least 2 other companies, I cannot find their data in my current files.
Optical Technologies has two devices: a manual spectrometer (you dial in the wavelengths) and a computer controlled one (via RS232 cable). Both sell for around $10K or less (prices may have risen since I last spoke to them) but they provide good value for money. As I remember, they also had a focusable pinhole to eliminate stray light.... a very desirable feature.
If you have not had much experience in microspectrophotometry, might I suggest Horst Piller's book Microscope Photometry, ISBN 3-540-08094-5; Spring Verlag, publisher, 1977. If you can't get it on the open market, call Irv Toplin at Zeiss and see if he can track down a copy either here in Thornwood or in Germany. I used to be one of two microspectrometry field specialists with Zeiss and found this book valuable. It stresses the importance of a stablized light source (if not available from your manufacturer, see Mel Dekker at Optiquip), aperturing, optical and statistical errors, etc.
Also interesting: A book edited by Michael Morris and put out by Marcel Dekker. My copy is not here today, so I can't give you the complete information but I think the title is Spectroscopic and Microscopic Imaging of the Chemical State. While the first chapter on microscopy is a bit weak (Dr. Morris and I have discussed that), the rest of the book is great.
Caveat: I have no commercial interest in any of these products, only an interest in promoting better use of microscopy in general.
Hope that all this helpful.
Best regards
Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 03:56 PM 4/20/99 -0400, James Martin wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Malc asks ... } } } Hi all! } I could do with a bit of serious technical advice } from someone with experience in preparing diffraction crystals. } ... } I've pillaged the diffracting crystals from an old Phillips } spectrometer that we have sitting in the basement. ...
I have to wonder if these crystals could be used with the JEOL Rowland circle?? ... a focussing issue, whereby the xtal surface curvature is focussed on the opposite side of the circle.
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
Hi Richie, Quartz & Silice is now a part of the French conglomerate Saint-Gobain.
best regards, mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear microscopists: } } I have following recipe to mix the four components for EPON 812. } For 25grs we use } 11.556 g Epikot } 7.125 g Dodecanylsuccinateanhydride, } 6.275 g Methylnodicanhydride } and 0.375 g DMP-30 (2,4,6 Triphenol) } } Well, anyone who uses other formulas to mix Epon or do you agree with my } } formula? } I would be happy to receive other propositions to mix Epon or any } further information on this stuff. } It is the first time I try Epon while using the 3-component ARALDITE } normally. } } Thank you for hints and tips, } keep trying ... } } Michael Reiner } } } Hi, When trying something new with embedding media, it might be best to go to a formulation which has been existence for 30 years - the formulations by Luft. These formulations have been used for millions of embedments with success. Look in any TEM textbook and you will find them. The "medium hard" formulation is a favorite of pathologists, because it is versatile and will embed many samples satisfactorily. By the way - It is not necessary to weigh out epoxides. Use disposable syringes for measuring accurately. You will not be able to detect a difference in block properties between monomers carefully weighed and carefully measured. I have data to support this. Hildy Crowley {hcrowley-at-du.edu}
I have a Hitachi 2500C, S/N 32001622-5303, with a Large Stage C and I am looking for a computer controllable stage controller. Do you have one that is available or do you know where I can obtain one. I would prefer an RS 232 interface but that is not absolutely necessary. I am also looking for a video converter to put the picture on the WEB like an Web Cam. I also have a Sigma system # 20400, customer #8294-01. I would also like to put the X-ray maps on the web in real time. I have also sent this message to Hitachi and Kevex and will be posting it on some other Web pages.
Dennis Coad Boeing Huntsville Central Labs Lead MS: JW-56 (256) 461-2976
} -----Original Message----- } From: ckuzmiak-at-chuma.cas.usf.edu [SMTP:ckuzmiak-at-chuma.cas.usf.edu] } Sent: Friday, April 16, 1999 10:19 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: How can serial sections be picked up } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Colleagues ..... } } This one is out of my area of expertise... Please reply direct to } the sender... } } Email: ckuzmiak-at-chuma.cas.usf.edu } Name: Carolyn Kuzmiak } School: USF } } Question: How can serial sections be picked up onto a formvar/carbon } coated } large slot grid? So far I have had problems with wrinkling of sections, } sections drying down onto metal instead of remaining in the slot window. } } -------------------------------------------------------------------------- } - [MORETZ,DR,ROGER TX BIPUS]
Jumping in a little late, the technique I use is not totally unlike the that described by Hildegarde Crowley, but may be sufficiently so to justify the response.
I use the reverse action, anti-capillary forceps--the #5 style to hold the slotted grid, pre-coated with formvar and carbon. Using the anti-capillary forceps upside down allows you to hold the grid at an angle. This additional angle provides greater flexibility in bringing the grid under the sections, attaching the first section to the support film and drawing the grid and ribbon onto the grid along the length of the slot. Repulsion of the grid and the ribbon can be eliminated or at least controlled by zapping each grid (just prior to immersion in the boat) with a Zerostat. I have also used a demagnetizing coil (purchased from Ladd many, many years ago) to reduce the repulsive interactions. The other thing I have used to control placement of the ribbon and to ensure attachment to the grid over the slot is the use of a lash to manipulate the ribbon. Those are difficult to come by unless you have long lashes.
This technique sounds (and I guess it is) tedious, but I have used it to successfully section over 500um through an amyloid plaque--over 700 grids in all, and the only sections lost were when the forceps tips perforated a slot during staining!
Thanks to all of you who replied to my query about differentiating apotosis from necrosis in TEM. I really appeciate it! The replies are attached below. In addition to the references give, I also found useful Voume 46 of Methods in Cell Biology; Cell Death, edited by Lawrence .M. Schwartz and Barbara A. Osborne.
As it turns out, the cells I was looking at are neither apototic nor necrotic, but now appear to have a strange disorder. But that's another story...
The replies:
Differentiating apoptosis and necrosis morphologically is based primarily upon nuclear changes, although there are characteristic cytoplasmic changes as well. In general (note the wiggle words), necrotic cells swell and lyse, whereas apoptotic cells shrink and fragment. Chromatin in apoptotic cells forms electron-dense crescents at the nuclear envelope, then breaks up. Apoptotic cells fragment into "apoptotic bodies" that may contain bits of chromatin. A good place to see characteristic ultrastructural changes of apoptosis is in lymphoid tissues, where lymphocytes die via apoptosis and then are phagocytosed by resident macrophages (the ones that are sometimes called "tingible body macrophages" because of the staining properties of the apoptotic cell remnants in them.)
Differenting apoptosis from necrosis is a tricky deal. Apoptotic cells may undergo secondary necrosis, during which they swell and lyse. So, just because you see necrotic cells doesn't mean that they didn't die apoptotically. Like everything else, it's complicated; there is a continuum of change with apoptosis and necrosis at opposite poles and a lot of stuff in between!
There are lots of good reviews on this topic. The Aug 28, 1998 issue of Science had a special section on apoptosis, and on page 1302, there is a series of three electron micrographs of neurons undergoing apoptosis. One of the first reviews of the subject contains the best collection of micrographs I've found - "Cell death: the significance of apoptosis" in the International Review of Cytology 68:251-306, 1980. Another good review was in Amer J of Pathol 146:3-15, 1995. The title is "Apoptosis, oncosis, and necrosis: an overview of cell death."
Jane Fagerland
There's lots of stuff on t.e.m. of apoptosis in vertebrate cells (it's very popular in HIV, cancer, inflammation response etc) and much of it indicates visible nuclear changes but relative stability of cytoplasm compared with necrosis.
There was a review article (as a good starting point): Microscopical Study of Cell Death via Apoptosis by S. Verhaegen in MIcroscopy and Analysis, January 1998 pp5-7
But you could do a reference or citation 'trawl' on the authors: Kerr, J.F.; Wyllie, A.H. or Currie
Malcolm Haswell
In response to your question, I did some necrosis versus apoptosis questions concerning a mycobacterium ulcerans toxin question we had here, and two papers that were particularly helpful distinguishing the two were from Scanning Microscopy Vol. 10, No. 1, 1996 pages227-237,by E. Falcieri, et al, Different Approaches to the study of Apoptosis, and in the same journal, by Dini et al, pages 239-252, an article entitled Recognition and Phagocytosis of Apoptotic Cells.
Beth Fischer
Apoptosis: the molecular basis of cell death - Current Communications in Cell and Molecular Biology 3. L. D. Tomei and F. O. Cope editors. Cold Spring Harbor Laboratory Press 1991
Cell Death in Biology and Pathology. I.D. Bowen and R.A. Lockshin ed. Chapman and Hall publs.
John (Keoni) Hardy
The most "conventional" way to detect apoptosis is to look for DNA fragmentation using in situ hybridization probes. I would not recommend that you go down that path unless you really need this technique to work in your lab. There are many in situ probes available to detect apoptotic cells if you do decide this is what you want. The DNA fragmentation that occurs during apoptosis will produce "patterns" in the nuclear chromatin which researchers have used to identify apoptotic cells. However, this has the same pitfalls as other morphologic characterizations (mostly in proving this is what you are looking at).
You might try looking for ways to detect cytoplasmic cytochrome c or activated caspases (not easy yet).
Here are some reviews to read: Baker et al, 1996 Oncogene 12:1-9. Lincz, 1998 Immuno.and Cell Bio. 76:1-19. Van Engeland et al, 1998 Cytometry 31:1-9 Solary et al 1998 Cell Bio. and Toxicol 14:121-132. Green & Reed 1998 Science 281:1309-1312 Dickson 1998 Trends Biotechnol 16:339-342 Cai et al 1998 Biochem. Biophys Acta 1366:151-165. O'Brian 1998 J. Gen virol 79:1833-1845. Granville et al 1998 Lab Invest 78:839-913 Kuan and Passaro 1998 Arch Surg 133:773-775.
Paul Webster
Aloha, Tina **************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
We have a Debben stage on our Hitachi 2460N. It works well for us and has a RS-232 interface. Ours is interfaced to a Link ISIS EDS system. I would not care to program such a thing by myself. (I am getting too old for it.)
I would be interested in what you hear about putting the video on the web. I have played around a little with it myself, but keep running out of time. Other responsibilities keep calling.
The x-ray maps could be put on line in pseudo real time if the Kevex saves files to a standard imaging format and supports an FTP server (i.e., it is standard Windows 95 OS). We use Microsoft's Personal Web Server the WarFTP FTP server to offer up files in particular directories. We save the files in a format that others can view and/or retrieve, and they are available as soon as we hit the Save button. You can check us out at WWW.MARL.IASTATE.EDU and see for yourself. BTW, JPG and GIF formats are best for this applications. Your clients would need to set up a helper application to view TIFF files, plus the files get big, fast.
At 11:30 AM 4/21/1999 -0500, you wrote: } } I have a Hitachi 2500C, S/N 32001622-5303, with a Large Stage C and I am } looking for a computer controllable stage controller. Do you have one that } is available or do you know where I can obtain one. I would prefer an RS 232 } interface but that is not absolutely necessary. I am also looking for a } video converter to put the picture on the WEB like an Web Cam. I also have a } Sigma system # 20400, customer #8294-01. I would also like to put the X-ray } maps on the web in real time. I have also sent this message to Hitachi and } Kevex and will be posting it on some other Web pages. } } Dennis Coad } Boeing Huntsville } Central Labs Lead } MS: JW-56 } (256) 461-2976
---------------------------------------------------- Warren E. Straszheim 23 Town Engineering Iowa State University Ames IA, 50011-3232
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
The Northern California Society for Microscopy will hold a Biomaterials Symposium on May 6th at Roche Biosciences in Palo Alto. A complete program is attached for those interested in attending. The symposium will begin at 1:00 p.m.
A social hour is scheduled for 5:45 followed by a Mexican Fiesta dinner featuring chicken and beef fajitas and cheese enchiladas, black beans and Spanish rice. The cost of the dinner is $15.00 for members and $7.50 for students. Reservations should be made by May 3rd. Contact Greg Lum to make your reservation. Telephone (415) 338-1091 or email glum-at-sfsu.edu
Photo contest: Remember to bring a micrograph for consideration for the cover of our directory 'Scope'. The NCSM council will act as judges and the winner will receive $100.00
Vendors are invited to bring brochures and other information describing their products. Vendors, please remember to bring a one-sheet advertisement to be included in the new 'Scope'.
All attendees are encouraged to bring a buddy to the meeting.
Michael Reiner wrote ================================================ I have following recipe to mix the four components for EPON 812. For 25grs we use 11.556 g Epikot 7.125 g Dodecanylsuccinateanhydride, 6.275 g Methylnodicanhydride and 0.375 g DMP-30 (2,4,6 Triphenol)
Well, anyone who uses other formulas to mix Epon or do you agree with my formula? I would be happy to receive other propositions to mix Epon or any further information on this stuff. It is the first time I try Epon while using the 3-component ARALDITE normally. ================================================ The trade name Epon® is owned by Shell Chemical. Until approximately 1970, Shell produced a product called Epon 812 and was widely used through EM-land . But at about that date, Shell announced the discontinuation of Epon 812. Shell continues to make other Epon resin grades but unfortunately, none seem to have value for applications in EM.
There is now a range of Epon "substitutes". Most of the major EM suppliers of consumables and supplies have their own "brand" of this substitute. However, they do not come from the same source. And they might come close to approximating the original Epon (in some respects some of them might be superior in terms of infiltration) but they are not identical, either with respect to the original Epon or with regard to the variety of different "substitutes".
So I make this comment, which I hope is not considered out of place, because if indeed someone did have some of the original Epon 812 left in bottles (some claim to still be working off a stock pile they purchased nearly thirty years ago), it may or may not be performing as it would have in 1970. And since workers today are probably using "substitutes", if precise formulaes are going to be stated to the fourth and fifth significant figures , then probably it would be appropriate to state precisely which "substitute" is being used for the resin component.
In our own laboratory at least, we are constantly changing the formula where we are seeking to end up with a hardness more appropriate for specific samples. So in our case, the "formula" can vary by some considerable amount
WASHINGTON (AP) - A single-celled microbe large enough to be seen with the naked eye has been found by researchers sampling ocean dredgings in the South Atlantic.
The bacterium - as big as the period at the end of this sentence - is the largest ever identified.
The microbe, discovered near Namibia, lives by absorbing sulfur and nitrates, and it swells as the chemicals are stored inside its cell walls, researchers report in a study published today in the journal Science.
The biggest of the bacteria is 0.75 millimeter, according to a report by Science.
Researchers at the Max Planck Institute for Marine Microbiology, who made the discovery, said the microbes form chain-like colonies that tend to glow from the absorbed nitrates.
``They look like a thin string of pearls,'' said the scientists, who named the new microbe Thiomargarita namibiensis, which means ``Sulfur Pearl of Namibia.''
``If the largest Thiomargarita was a blue whale,'' Science said in a statement, ``then an ordinary bacterium would be a bit smaller than a newborn mouse.''
In this analogy, the largest previously known bacterium ``would be about as big as a lion,'' about 100 times smaller than Thiomargarita, Science reports. The previous record holder was Epulopiscum fishelsoni, a microbe found in the gut of surgeonfish.
Max Planck researchers said the bacteria live in an environment with high levels of hydrogen sulfide, conditions that are toxic to most other forms of life.
The scientists said Thiomargarita is found in great concentrations in Namibian coastal sediments that contain high levels of toxic sulfide.
If we could use these in dumps and what have you it could do a lot.
Gordon
Gordon Couger gcouger-at-couger.com www.couger.com/gcouger Stillwater, OK 405 624-2855 GMT -6:00
CMP Cient=EDfica and Philips are holding a joint colloquium on Microscopy an= d Materials Analysis in Madrid on 26th May 1999. The colloquium is aimed at all Microscopists and analysts working on both materials and biological applications. The object is to discuss new techniques and instrumentation that will aid us in characterisation, and technologies that can speed up sample preparation.
Working languages will be Spanish & English.
=46or more details see www.cmp-cientifica.com or e-mail me direct.
MAMAS will be meeting at NIST on Tuesday, May 18, 1999 from 10:30am until 3:00pm. There will be coffee and doughnuts and two speakers. All are welcome. See below for details.
Hope to see you there, -- John Henry
John Henry J. Scott NIST Microanalysis Research Group http://www-sims.nist.gov/Division/Contacts.html
ANNOUNCEMENT ------------
Mid-Atlantic Microbeam Analysis Society (MAMAS) Meeting
at the National Institute of Standards and Technology (NIST) Gaithersburg, MD
on
Tuesday, May 18, 1999 10:30am -- 3:00pm
Lecture Room D, Administration Building
10:30am: Coffee and Doughnuts
10:45am: Dave Wollman, NIST (Boulder) "Microcalorimeter EDS with 3 eV Energy Resolution"
Noon: Lunch
1:15pm: Jan S. Iwanczyk and Bradley E. Patt, Photon Imaging, Inc. "High Count Rate and High Energy Resolution Silicon Drift Detectors for X-Ray Microanalysis"
directions to the NIST campus can be obtained from the NIST website at: http://www.nist.gov/public_affairs/maps/nistmaps.html
for more information, contact John Henry Scott (NIST): (301) 975-4981 (301) 417-1321 FAX email: johnhenry.scott-at-nist.gov
Hello to everyone, A colleague just called me with a very interesting question. Does anyone know of a way to assess the possible leakiness of blood vessels in skin samples from archived tissue. This tissue was not formalin fixed, so antigenic sites should be fine.
Ramin Rahbari PARKE-DAVIS Pharmaceutical Research Pathology and Experimental Toxicology 2800 Plymouth Road Ann Arbor, MI 48105 Office (734) 622-3383 Fax (734) 622-5001 Ramin.Rahbari-at-WL.COM
The discussion regarding WPE values for the Epon substitues was basically correct. WPE values used to be very important for creating blocks of a standard set of mechanical and chemical properties during the time when Shell Epon WPE values ranged between 142 and 165. The same formulation used without taking these differences into account made enormous difficulties in the 60's or as long as labs used the Shell product. If anyone today is buying a substitute which varies significantly from batch to batch in WPE values, I urge them to change suppliers immediately. Not all substitutes are created identically. Some contain dilutents, plasticicers, Araldite, etc. All this is proprietary information. It cannot be said that one substitute is better than any other. It is important to stick to one substitute, learn its characteristics, and make adjustments. Should anyone have a problem with the mechanical or chemical characteristics of an 812 substitue, please contact me at {hcrowley-at-du.edu} . I have an entire notebook full of formulations based on WPE values dating from a time when I was faced with an extremely difficult embedding problem. I may have some valuable information. Bye, Hildy Crowley University of Denver Denver, CO
I have a broken Varian 30 L/s ion pump that needs repair or replacement/exchange. Anyone have any experience with reputable firms that perform this service? Would appreciate hearing about sources.
I have question concerning cleaning of white ceramic insulator inside electron gun of TEM JEOL 2000FX. As a consequence of electric charges thereare brown traces on the surface of insulator. Does anybody has experience how to remove these traces and what cleaning solution (e.g. aceton, methanol or something else) to use. Thank you very much.
Yours sincerely
Peter Makroczy
Dept. of Mat.Science
Technical University of Kosice
Slovak Republic
makroczy-at-tuke.sk
-- Peter Makroczy Technical University of Kosice Department of Materials Science Park Komenskeho 11 042 00 Kosice Slovak Republic E-mail: makroczy-at-tuke.sk Tel.: +421 95 602 25 40 Fax.: +421 95 633 27 23
On Fri, 23 Apr 1999 08:47:25 +0200 Peter Makroczy {makroczy-at-tuke.sk} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear microscopists, } } I have question concerning cleaning of white ceramic insulator inside } electron gun of TEM JEOL 2000FX. As a consequence of electric charges } thereare brown traces on the surface of insulator. Does anybody has } experience how to remove these traces and what cleaning solution (e.g. } aceton, methanol or something else) to use. Thank you very much. } } Dear Peter,
We have successfully cleaned these by rubbing with alumina powder (Al2O3) in alcohol as a paste on a tissue or cloth. However, it is important that you remove all traces of the alumina powder when you have finished.
Unless it is a very small mark which is easily accessible we would completely dismantle the gun and work on the insulator alone. After cleaning off the insulator marks wipe and blow off any excess powder with a clean gas supply (N2 from a cylinder not compressed air which usually has oil in it). Then wash it in alcohol in an ultrasonic bath changing the alcohol at least 3 times.
If there are a lot of discharge marks or they are very bad then grit blast it to clean it instead of rubbing with alumina paste. Wash it thoroughly afterwards as described.
Sometimes it is not possible to clean out very deep discharge tracks and the insulator needs to be replaced.
If you are dubious about carrying this out I know that JEOL(UK) clean customers guns by this method when reconditioning them. I am also fairly certain that they would recondition yours (but I don't know the cost).
Good luck, Ron
---------------------- Ron Doole Dept. of Materials, University of Oxford, Parks Road, Oxford. OX1 3PH. ron.doole-at-materials.ox.ac.uk
Never found any solvent for my similar stains. On my Etec, they have to be rather bad to cause a problem. Anyway, I did clean the glazed porcelain insulator (to a degree) using 1/4 micron diamond paste and a small, high speed felt wheel ("Dremel Tool"). Followed with UT in detergent/warm water, water rinse, isopropanol rinse, blow dry w/N2. This was the first serious cleaning in 15 years. Never could determine any performance difference - only cosmetic.
} From Microscopy-request-at-sparc5.microscopy.com Thu Apr 22 20:02:53 1999 } } Date: Thu, 22 Apr 1999 16:33:28 -0700 } To: MSA listserver {Microscopy-at-Sparc5.Microscopy.Com} } From: "Dr. Gary Gaugler" {gaugler-at-calweb.com} } Subject: Ion pump repair sources? } } ---------. } } } I have a broken Varian 30 L/s ion pump that needs repair } or replacement/exchange. Anyone have any experience } with reputable firms that perform this service? Would } appreciate hearing about sources. } } Cheers, } Gary Gaugler, Ph.D. } } Gary, From your email address, I assume you are located in the US. I think that Duniway stockroom has an excellent reputation in this area. They can be contacted at 1-800-446-8811 or at www.duniway.com. They are located in Mountain View, CA but with the excellent UPS and Fedex and other services we enjoy nowadays it makes little difference where they are, once we have gone to the trouble of boxing up the item. Suggest you post a summary of your responses.
Don Marshall
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology "A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)
Hello everyone. I'm looking for a diamond scribing attachment for old Zeiss compound scopes that was used to score areas on slides. It attached to the objective ring like a lens, and when you found the object of interest, you swung this scribe around and turned it to etch a circle at that spot. The one I have used was in Mel Fuller's lab at UGA, but it disappeared when he retired (sound familiar?). It could be adjusted for any diameter circle. It may not necessarily be specific for a Zeiss: that was the scope I used it on. I can't remember the labels or markings on it now. It was extremely useful for flat-embedded material that was to be cut out and glued to blank BEEMs for sectioning.
If you know where I can find one, let me know how to purchase it.
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
The matter of cleaning parts from the vacuum systems of electron microscopes, including ceramic insulators and the interiors of electron gun chambers, is discussed in some detail on pp. 69 - 75 of my book 'Vacuum Methods in Electron Microscopy' (see: http://swww.2spi.com/catalog/books/book48.html and http://www.bookshop.co.uk/portland/).
A procedure we have found to be satisfactory for cleaning the guns of TEMs operating at accelerating voltages up to 200 kV is as follows: Rub the interior of the gun chamber and the ceramic insulator with lint-free, grease-free cloth pads that have been dipped in a thick slurry of reagent grade isopropyl alcohol and either finely ground calcium carbonate powder or the common 0.05 micrometer grade of aluminum oxide metallographic polishing powder. After all areas are cleaned in this way they are wiped repeatedly with clean pads moistened only with isopropyl alcohol to remove the polishing powder. Then all surfaces, corners,and joints are blasted with a clean, dry gas blaster to remove any residual particles. The insulator and interior of the gun should be heated with a clean hair dryer to remove as much residual surface moisture as possible just before the gun is reassembled.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
As I recall, the first commercial model TEM produced by RCA was called the model EMB. (Model EMA was an experimental model that was never sold) I believe this model was introduced sometime around 1940. I took my first electron micrographs on the second one of these instruments sold. It was located in the laboratory of Prof. Robley Williams, in the Physics Department here at the U. of Mich. There is a brief discussion of this model, and a picture of it on p. 205 of the First Edition of Cecil Hall's book, 'Introduction to Electron Microscopy'. McGraw-Hill, 1953.
According to Hall, RCA introduced it's model EMU microscope in 1944. A picture of this instrument is shown on p.206 of the above book. The RCA model EMU2 looked the same as the EMU, shown in this picture. I know, because i did a lot of work on one of these instruemnts in the laboratory of Dr. Thomas Francis, in the School of Public Health here at the Univ. of Mich.
After the EMU2 model, and starting with model EMU3, RCA changed over to an more user-friendly design which was carried through in the EMU4 instruemnts with little modification. A picture of an RCA EMU3 microscope is shown on p.179 of the Second Edition of Hall's book (1966). I don't know exactly when the EMU3 microscopes were introduced, but it was sometime in the early 1950s. RCA went out of the business of producing electron microcopes in 1969. Again, I know, because I was President of EMSA that year, and had the sad task of dealing with this at our annual meeting that year. I believe the current model at that time was the EMU4, so it was probably introduced sometime shortly after 1966.
This is the best info I can come up with off-hand. Somewhere I have a page from an RCA bulletin showing all their instruments and giving dates of production, but I don't seem to be able to find it among the clutter in my office. However, If you want more detailed info, I'll go through my files at home and see if I can find it there. However, It is likely that you have an EMU or EMU2 model (they look alike). I'd be very surprised if any of the EMBs are still in existance.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
Has anyone made a biprism for a TEM using a drawn SiO_2 coated with Au?
I am looking for ideal fibre thickness, heat source that was used (which gases & flame temperature) and for any tips on how to make them. The current suggestions that I have heard are an H_2 and O_2 torch to get the temperature up to aroung 2000 K, but these gases can be quite hazardous to work with.
I would really appreciate any advice.
Thanks, Jon
-- ***************************************** Jonathan Barnard
Microstructural Physics, H.H.Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol BS8 1TL.
I experienced last year the same problem with the electron gun of my JEOL 2000FX. The brown traces are provoqued by the discharges inside the freon gas. To clean these traces, don't use any solution because the ceramic is porous and after opening the gun, you will have to purge several times the atmosphere of the gun because of the oxygen and the water vapor contained in the air. For this reason it is not recommended to put solution on the ceramic. To clean the ceramic, we used a stick of ceramic like an eraser. After removing the main part of the brown traces, we cleaned with a tissue with alcohol and put the gun into a furnace at 100=B0C for some hours. Then we remount the gun, after we had cleaned the metallic chamber from the traces of discharges (white spots on the steel) with POL paste. You should fill the atmosphere of the gun with nitrogen and purge two or three times and finally fill the gun chamber with freon or SF6. To evacuate the gun there is a valve behind the microscope near the pressure gauge of the gun. You can find the position to use if you look into the manual. This is to clean the ceramic part that is in the freon atmosphere (were the polarisation resistances are located) if you want to clean the ceramic part inside the column, it is more complicated because you don't have access to all electrodes.
That's all! But for us this cleaning was not sufficiant and we had to exchange the gun that was fortunatelly under contract.
Eric LEROY Dr. Laboratoire de Chimie Metallurgique des Terres Rares UPR 209 - CNRS Groupe des Laboratoires de Thiais 2-8, rue Henri Dunant 94320 THIAIS cedex
Dear Peter, I would recommend you ask the JEOL service engineers first. The only time I tried to clean the insulator on a TEM gun, I tried Wenol metal polish first. This removes some of the diffusion pump oil deposit, but did not get off the brown marks, so I next tried a diamond paste (6 micron). That did work. Remove this thoroughtly with several washes of clean ethanol. You wrote:
} } Dear microscopists, } } I have question concerning cleaning of white ceramic insulator inside } electron gun of TEM JEOL 2000FX. As a consequence of electric charges } thereare brown traces on the surface of insulator. Does anybody has } experience how to remove these traces and what cleaning solution (e.g. } aceton, methanol or something else) to use. Thank you very much. } } } Yours sincerely } } } Peter Makroczy } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Peter- I have used Micro-90 for the past couple years with good results. manufactured by: International Products Corp. PO Box 70, Bulington, NJ 08016-0070, USA phone: 609-386-8770 UK Branch: 1 Church row, Chislehurst, Kent BR7 5PG, United Kingdom Phone: 0181-467-8944 -Mike
On Fri, 23 Apr 1999, Peter Makroczy wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear microscopists, } } I have question concerning cleaning of white ceramic insulator inside } electron gun of TEM JEOL 2000FX. As a consequence of electric charges } thereare brown traces on the surface of insulator. Does anybody has } experience how to remove these traces and what cleaning solution (e.g. } aceton, methanol or something else) to use. Thank you very much. } } } Yours sincerely } } } Peter Makroczy } } Dept. of Mat.Science } } Technical University of Kosice } } Slovak Republic } } makroczy-at-tuke.sk } } -- } Peter Makroczy } Technical University of Kosice } Department of Materials Science } Park Komenskeho 11 } 042 00 Kosice } Slovak Republic } E-mail: makroczy-at-tuke.sk } Tel.: +421 95 602 25 40 } Fax.: +421 95 633 27 23 } } } }
The consensus is correct. Duniway is a good place for pump repair and exchange. They charge $495 for an exchange and it is essentially next day if a like model is in stock. Otherwise they repair the unit they receive. They have the same Varian 30 L/s pump I have in stock so I should receive the exchange unit on Monday or Tuesday.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Jon:
We use an H2/O2 torch for blowing quartz fibers to make biprisms. I believe most other practitioners in the field do the same.
Check out "Practical aspects of electron holography", D. C. Joy et al, Ultramicroscopy 51(1993) 1-14 for further tips. It is a good idea to examine the fiber in the SEM after it is mounted and coated (we do a simple Au-Pd sputter coating), to assure that it is smooth, uniform, and the correct diameter. It takes practice, and there are many techniques for blowing and collecting fibers. Finally, our health and safety guys made us build a special hood with proper ventilation before we were allowed to perform this operation. You may want to check things out in this area at your place...
Best wishes,
Larry PS I'll send you a reprint...
} } } Has anyone made a biprism for a TEM using a drawn SiO_2 coated with Au? } } I am looking for ideal fibre thickness, heat source that was used (which } gases & flame temperature) and for any tips on how to make them. } The current suggestions that I have heard are an H_2 and O_2 torch to } get the temperature up to aroung 2000 K, but these gases can be quite } hazardous to work with. } } I would really appreciate any advice. } } Thanks, Jon } } -- } ***************************************** } Jonathan Barnard } } Microstructural Physics, } H.H.Wills Physics Laboratory, } University of Bristol, } Tyndall Avenue, } Bristol BS8 1TL. } } 0117 928 9000 ext 8750 } } *****************************************
Dr. Lawrence F. Allard Senior Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
I have 2 suggestions that may be of interest to you:
1) MicroDrill The MicroDrill is a small drill that fits into the objective port of your=
microscope and uses the rack system on your microscope to turn it into a micro drill press. You can either "drill" holes (60u) or "scribe" circle= s of diameters from 500 to 1500 microns. The drill is battery operated. T= he drill mounts into a 36 TPI and 0.8" diameter opening. If you have anothe= r size, we can work out an adapter.
2) MicroMarker The MicroMarker is a small inked marker that will fit in place of your standard objectives in the light microscope and comes in a kit with 3 colors. There is one kit for 33mm long objectives and one kit for 45mm long objectives. They will make a circle with a 0.07" ID. =
If you would like more inforamtion, please contact me off-line.
Best regards-
David Henriks South Bay Technology, Inc. 1120 Via callejon San Clemente, CA 92673 USA
Hello everyone. I'm looking for a diamond scribing attachment for old Zeiss compound =
scopes that was used to score areas on slides. It attached to the =
objective ring like a lens, and when you found the object of interest, =
you swung this scribe around and turned it to etch a circle at that =
spot. The one I have used was in Mel Fuller's lab at UGA, but it =
disappeared when he retired (sound familiar?). It could be adjusted for =
any diameter circle. It may not necessarily be specific for a Zeiss: =
that was the scope I used it on. I can't remember the labels or =
markings on it now. It was extremely useful for flat-embedded material =
that was to be cut out and glued to blank BEEMs for sectioning.
If you know where I can find one, let me know how to purchase it.
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
Try the great folks at Duniway http://www.duniway.com
I don't have experience with a 30 l/s pump, but the pumps with smaller flanges have to be cut apart and then rewelded, which limits the number of times they can be rebuilt.
best regards mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
The M & M '99 Golf Tournament is scheduled for July 31, 1999 in the Columbia River Gorge outside Portland. Space is limited to the first 50 players. Please send you golf reservation forms in as soon as possible. If you do not have a registration form you may call 877-MSA-MAS-1 (877-672-6271)and ask for one, or you may e-mail me at Ladd Research (jarnott-at-ladd.cc) with a fax number and I can fax you a registration form. This is going to be our best tournament yet, so hurry to get your slot.
Thanks, John Arnott Chairman -- LADD RESEARCH 13 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
I was asked to post this for a friend.=A0 Please reply directly to him = at the e-mail below as he is not part of this listserver.=A0 Thank-you!
Marisa
---------------
I am looking for information on any training program that would include = TEM sample preparation. I am mostly interested in the wedge polishing = technique.
I have recently acquired an old PGT IMIX EDS system. It appears the hard drive in the Sparc 1 system is dead. I am told a replacement is nowhere to be found. Is there anyone out there who could help me locate a replacement drive? If not a drive, does anyone have a computer and analyzer for sale which is compatible with a PGT Prism digital spectrometer? Michael Ingram Rodel, Inc Polishing Lab 451 Bellevue Rd Newark, DE 19713 (302) 366-0500, ext.: 2545
Can someone recommend a good instructional video for light microscopy suitable for a graduate laboratory?
Thanks,
Susan A. Fugett
Department of Chemical Engineering and Materials Science Phone: 612-625-8803 University of Minnesota 612-625-0808 421 Washington Ave SE Fax: 612-626-7246 Minneapolis, MN 55455 Email: fugett-at-cems.umn.edu
Peter: My Hitachi engineer taught me to use acetone to wipe this ceramic insulator. I have never had very stubborn deposits, so I do not know what additional cleanig would be suitable in that case.
Maureen Petersen
} Dear microscopists, } } I have question concerning cleaning of white ceramic insulator inside } electron gun of TEM JEOL 2000FX. As a consequence of electric charges } thereare brown traces on the surface of insulator. Does anybody has } experience how to remove these traces and what cleaning solution (e.g. } aceton, methanol or something else) to use. Thank you very much. } } } Yours sincerely } } } Peter Makroczy } } Dept. of Mat.Science } } Technical University of Kosice } } Slovak Republic } } makroczy-at-tuke.sk } } -- } Peter Makroczy } Technical University of Kosice } Department of Materials Science } Park Komenskeho 11 } 042 00 Kosice } Slovak Republic } E-mail: makroczy-at-tuke.sk } Tel.: +421 95 602 25 40 } Fax.: +421 95 633 27 23
************************************************************************ Maureen Petersen Department of Plant Pathology 1453 Fifield Hall University of Florida
LATE BREAKING POSTER SESSION AT MICROSCOPY AND MICROANALYSIS '99
Microscopy and Microanalysis '99 will feature a poster session composed of presentations of newly acquired data or analyses which were unavailable for submission by the February 15 deadline. A short, half page abstract describing the studies is required. The abstract should include: Title, Authors, Authors affiliation, and a Brief Description of the studies. The description should include the Aim of the studies, a short characterization of the Methods, and a brief account of the Results and their Importance. Abstracts should be e-mailed or faxed to the program chair, Jay Jerome, at jjerome-at-wfubmc (email) or 336-716-6174 (fax). Abstracts may be submitted immediately but must be received by June 25, 1999. Abstracts will be reviewed by members of the program committee. A limited number of poster boards are available and preference will be given to early submissions. Abstract authors will be notified of acceptance of their abstracts no later than July 1 (earlier for early submissions).
Additional Information on M&M'99 can be found on the meeting WWW Site
---------------------------------------------- - AKA: W. Gray Jerome, Ph.D. - - Department of Pathology - - Wake Forest University School of Medicine - - Winston-Salem, NC 27157-1092 - - Ph: 336-716-4972, 336-716-2675 - - Fax: 336-716-6174 - - E-mail: jjerome-at-wfubmc.edu - ----------------------------------------------
John, The item is called " Object marker with diamond" and is a Zeiss item # 462960 and was $960 in the 1991 catalog.I find it very useful. Russ, Xerox
-----Original Message----- } From: John Shields [mailto:jpshield-at-arches.uga.edu] Sent: Friday, April 23, 1999 9:08 AM To: msa listserver
Hello everyone. I'm looking for a diamond scribing attachment for old Zeiss compound scopes that was used to score areas on slides. It attached to the objective ring like a lens, and when you found the object of interest, you swung this scribe around and turned it to etch a circle at that spot. The one I have used was in Mel Fuller's lab at UGA, but it disappeared when he retired (sound familiar?). It could be adjusted for any diameter circle. It may not necessarily be specific for a Zeiss: that was the scope I used it on. I can't remember the labels or markings on it now. It was extremely useful for flat-embedded material that was to be cut out and glued to blank BEEMs for sectioning.
If you know where I can find one, let me know how to purchase it.
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu ********************************************
As the manufacturer of the "MicroDrill" a microscope marking tool, I feel that I should point out one of its limitiations that was not mentioned in David Henriks reply to John Sheilds.
Unlike the earlier Zeiss product, scribes made with the "MicroDrill" are not continuously adjustable for size. Individual bits of the desired scribe diameter must be substituted into its chuck to achieve various scribe sizes.
SUMMER I 1999 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-Section BA)
NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York
A five week, Summer Session I 1999 semester, course in Biological Transmission Electron Microscopy is being offered by the Biology Department of Nassau Community College. This is a 4 credit course offered four days per week (Monday through Thursday) between the hours of 8:00 am and NOON. Classes will begin on May 24 and end on June 24, 1999.
This is a "hands-on" course emphasizing biological specimen preparation, ultra-thin sectioning involving block trimming, glass knifemaking and operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III), thick and ultra-thin section mounting and contrast staining (UA and Pb citrate), grid support films (formvar, carbon), student operation of the TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs through the process of black & white photography, and electron micrograph analysis. Students will work on a chosen sample(s) with the goal of producing a portfolio of high quality TEM photomicrographs of that sample(s).
The course is widely transferrable and the cost per credit is reasonable at $86 per credit (for Nassau County residents or New York State residents with a certificate of residency).
More information about the Bio-Imaging Center at NCC, course descriptions and syllabi, and the beginnings of a student gallery of EM photomicrographs is available at our web site. The URL is {http://www.sunynassau.edu/webpages/biology/becks.htm} .
Interested individuals should register as soon as possible since the course is limited to a total enrollment of ten (10) students.
If you have further questions, you should e-mail me directly at the address below.
For information about mail or telephone registration (Dial-a-Course) point your browser to http://www.sunynassau.edu/courses/sum99/sum99.htm. Telephone registration is only available until April 29, 1999 (between the hours of 2:30 pm to 7:00 pm) The phone numbers are (516) 572-7131 or 7372 or 7425.
Stephen J. Beck Associate Professor Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: {becks-at-sunynassau.edu} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
Leitz also made the same item you are talking about that would screw into a standard nosepiece. This may still be available. if you are interested in something to do the same thing that leave a ink circle around the area then I would check with Nikon rep. this does the same thing at a fraction of the cost, Maybe $200 or so opposed to $500 or $600. I may be wrong on my estimates but it is a siginificant difference. Diamond scriciber opposed to ink???
This item is used by cytologist to mark areas of interest on slides typically typically with say 10x objective then move up to 40x high dry...
Good luck
C. Passione -----Original Message----- } From: Gillmeister, Russ {RGillmeister-at-sdms.usa.xerox.com} To: 'John Shields' {jpshield-at-arches.uga.edu} Cc: 'MSA' {Microscopy-at-sparc5.microscopy.com}
} Can someone recommend a good instructional video for light microscopy } suitable for a graduate laboratory? } You'll find a good CD-ROM reviewed in the Project MICRO bibliography; URL below. It's Pagliaro et al., "Microscopy Tutor" It's definitely university level & is described in MICRO to discourage precollege teachers from ordering it.
Thjere's a recent 15 minute video on Koehler illumination: Matsudaira, "Getting Started", from the Using Microscopy series. It's from Cogito, www.cogitomedia.com & is distributed by Academic Press.
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
If indeed the "Ink" Objective Marker is suitable for the application. Nikon still has them... #79032 and the List Price is $137.00. They are RMS thread and refillable with various ink colors.
Regards,
Larry Kordon Nikon, Inc. Columbia, Maryland nikon-at-jagunet.com
Cono Passione wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } John, } } Leitz also made the same item you are talking about that would screw into a } standard nosepiece. } This may still be available. if you are interested in something to do the } same thing that leave a ink circle around the area then I would check with } Nikon rep. this does the same thing at a fraction of the } cost, Maybe $200 or so opposed to $500 or $600. I may be wrong on my } estimates but it is a } siginificant difference. Diamond scriciber opposed to ink??? } } This item is used by cytologist to mark areas of interest on slides } typically typically with say } 10x objective then move up to 40x high dry... } } Good luck } } C. Passione } -----Original Message----- } } From: Gillmeister, Russ {RGillmeister-at-sdms.usa.xerox.com} } To: 'John Shields' {jpshield-at-arches.uga.edu} } Cc: 'MSA' {Microscopy-at-sparc5.microscopy.com} } Date: Friday, April 23, 1999 9:14 PM } Subject: RE: searching for special lens } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } John, The item is called " Object marker with diamond" and is a Zeiss item } # } } 462960 and was $960 in the 1991 catalog.I find it very useful. Russ, Xerox } } } } -----Original Message----- } } } From: John Shields [mailto:jpshield-at-arches.uga.edu] } } Sent: Friday, April 23, 1999 9:08 AM } } To: msa listserver } } Subject: searching for special lens } } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hello everyone. } } I'm looking for a diamond scribing attachment for old Zeiss compound } } scopes that was used to score areas on slides. It attached to the } } objective ring like a lens, and when you found the object of interest, } } you swung this scribe around and turned it to etch a circle at that } } spot. The one I have used was in Mel Fuller's lab at UGA, but it } } disappeared when he retired (sound familiar?). } } It could be adjusted for } } any diameter circle. It may not necessarily be specific for a Zeiss: } } that was the scope I used it on. I can't remember the labels or } } markings on it now. It was extremely useful for flat-embedded material } } that was to be cut out and glued to blank BEEMs for sectioning. } } } } If you know where I can find one, let me know how to purchase it. } } } } ******************************************** } } John P. Shields } } Center for Ultrastructural Research } } 151 Barrow Hall } } University of Georgia } } Athens, GA 30602-2403 } } (706)542-4080 } } jpshield-at-arches.uga.edu } } ******************************************** } } } } } }
We have to reluctantly part with our Zeiss (Leo) 902 TEM with Integrated Electron Energy Spectrometer, to make way for a new TEM. Any one interested in acquiring it can get in touch with me directly through E-mail (mvp2-at-cornell.edu). Information on the 902 is provided below:
ZEISS (LEO) 902 TEM. The unit is in excellent condition and is equipped with: integral EELS prism and spectrometer and recorder, for both one and two dimensional (quantitative) EELS imaging, a low light level (SIT) video camera and monitor, a motorized goniometer stage capable of specimen tilt through 120 degrees, and full rotation; motorized specimen movement, and a plate camera. Date acquired 10/01/89 Original cost of the unit $235,750 The unit has been under service contract since it was installed.
Dear nestor please forward this message to microscopy :
Dear netter,
All my post to microscopy was rejected and I and/or my provider (what is very small company) have been unjustify accused as a source of spam, please be so kind and send to me directly if you have similar problem.
Is there any chance to punish individuals for this accusation?. Is it custom in america that you can be taken into jail for pederesty or muder only that you have similar (not same) name or address to some criminal?
Keep care and be of good cheer.
Regards
(name) Vratislav Richard Eugene Maria John Baptiste (surname) of Bejsak (Bayshark)-Collorado-Mansfeld
http://www.coleoptera.org Coleoptera - Australia, Tenebrionidae of World (incl. Lagriinae, Alleculinae)
University of Sydney The Wentworth Bldg., P.O.Box 62 NSW 2006 AUSTRALIA phone : +61 414 540 465 e-mail: vratislav-at-bigfoot.com ricardo-at-ans.com.au (before Ricardo-at-compuserve.com and ricardo-at-login.cz )
Only after the last tree has been cut down, only after the last river has been poisoned, only after the last fish has been caught, only then will you find that money can not be eaten.' CREE INDIAN PROPHECY.
Your accuser was text in your header. No individual per say. Your Email contained information which matched to the automatic filter in the listserver. This is something that everyone has to put up with to protect the greater good of the ListServer community. There are a number of checks and balances that have been put into the system over the years to minimize SPAM/JUNK Mail and Viruses. Your message got flag as a POTENTIAL source, it was stopped after you replied as per the instructions it subsequently was passed through to the list after HUMAN review. Yes this caused a delay in your posting, however, the number of Undesireable messages stopped by the filter is far greater than the few that are delayed. If you do not understand or appreciate this I am sorry, but you need to realize that thousands of people can be affected by inappropriate information in the Email. Maintaining and monitoring postings even if it is only by a software program is the appropriate thing and prudent job to do in today's environment.
If you wish to submit anyone's name to your lawyers it should be me as the ListServer SysOp. I am solely responsible for the installing and maintaining the filter as well as operating the server. I have setup the key word lists and I edit and maintain them. There are no other persons involved.
I accidentally ran into part of a Nova show on a PBS station showing some very interesting micro videos of cells, micro animals, and parasites, colorized SEMs, and fiber optic close-ups of insects and caterpillars. The series of 3 shows is called Odyssey of Life. I don't know if it is current issue Nova, but I think the pictures would interest many on the list. I missed the 1st show, saw part of the second. The 3d show is an interview with the maker, the person who made some fascinating human embryo pictures some years back.
Leonard Corwin Research Chemist Fort Dodge Animal Health Princeton, NJ 08543-0400
} } It is all attitude, } } } This is an actual job application someone submitted at a McDonald's } fast-food establishment AND THEY HIRED HIM! } (editor's note: If they hire this guy, I am SURE they would hire any of } us!!) } } } } NAME: Greg Bulmash } } DESIRED POSITION: Reclining. HA But seriously, whatever's available. If I } was in a position to be picky, I wouldn't be applying here in the first } place. } } DESIRED SALARY: $185,000 a year plus stock options and a Michael Ovitz } style severance package. If that's not possible make an offer and we can } haggle. } } EDUCATION: Yes. } } LAST POSITION HELD: Target for middle management hostility. } } SALARY: Less than I'm worth. } } MOST NOTABLE ACHIEVEMENT: My incredible collection of stolen pens and } post-it notes. } } REASON FOR LEAVING: It sucked. } } HOURS AVAILABLE TO WORK: Any. } } PREFERRED HOURS: 1:30-3:30 p.m., Monday, Tuesday, and Thursday. } } DO YOU HAVE ANY SPECIAL SKILLS?: Yes, but they're better suited to a more } intimate environment. } } MAY WE CONTACT YOUR CURRENT EMPLOYER?: If I had one, would I be here? } } DO YOU HAVE ANY PHYSICAL CONDITIONS THAT WOULD PROHIBIT YOU FROM LIFTING UP } TO 50 LBS?: Of what? } } DO YOU HAVE A CAR?: I think the more appropriate question here would be "Do } you have a car that runs?" } } HAVE YOU RECEIVED ANY SPECIAL AWARDS OR RECOGNITION?: I may already be a } winner of the Publishers Clearinghouse Sweepstakes. } } DO YOU SMOKE?: Only when set on fire. } } WHAT WOULD YOU LIKE TO BE DOING IN FIVE YEARS?: Living in the Bahamas with } a fabulously wealthy super model who thinks I'm the greatest thing since } sliced bread. Actually, I'd like to be doing that now. } } DO YOU CERTIFY THAT THE ABOVE IS TRUE AND COMPLETE TO THE BEST OF YOUR } KNOWLEDGE?: No, but I dare you to prove otherwise. } } SIGN HERE: Scorpio with Libra rising.
-- End original message --
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress ... But I repeat myself.-Mark Twain**
Thank you for many hints and formulations on Epon embedding. Now I know that I have to try out a few paths. However , meanwhile I=B4m aware of that the method of trial and error is possibly the most important basic technique in embedding business. In this case, I think I will use the formula that is traded from generation to generation in our lab (see my first mail ref. to Epon ..). Bye, Michael Reiner
To all compmed and histonet and microscopy members:
I goofed big time and I'm sorry about sending the non-related humor e-mail it was NOT intentional but I hit the wrong send key. please forgive...... now I gotta tell my boss to ignore my e-mail...
best regards, Bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis Dept. of Environmental Sciences and Engineering University of North Carolina CB#7400 Chapel Hill, NC 27599 Phone office 919-966-6343 Lab 919-966-6140 Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress .. But I repeat myself.-Mark Twain**
As a subscriber to the ListServer, I would like to express my appreciationn and support for all your efforts to minimize the SPAM/JUNK mail and viruses we receive.
Gill Bond New Mexico Tech
On Mon, 26 Apr 1999, Nestor J. Zaluzec wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Ricardo } } Your accuser was text in your header. No individual per say. } Your Email contained information which matched to the automatic filter in the } listserver. This is something that everyone has to put up with } to protect the greater good of the ListServer community. There } are a number of checks and balances that have been put into the } system over the years to minimize SPAM/JUNK Mail and Viruses. } Your message got flag as a POTENTIAL source, it was stopped } after you replied as per the instructions it subsequently was passed } through to the list after HUMAN review. Yes this caused a delay } in your posting, however, the number of Undesireable messages } stopped by the filter is far greater than the few that are delayed. } If you do not understand or appreciate this I am sorry, but you need } to realize that thousands of people can be affected by inappropriate } information in the Email. Maintaining and monitoring postings } even if it is only by a software program is the appropriate thing } and prudent job to do in today's environment. } } If you wish to submit anyone's name to your lawyers it should be } me as the ListServer SysOp. I am solely responsible for the installing } and maintaining the filter as well as operating the server. I have setup the } key word lists and I edit and maintain them. There are no other } persons involved. } } } Nestor } } } } } }
Dear Mr. Martin, S.E.E. manufactures turn-key microspectrophotometers for the UV, visible, and NIR regions. Our website is located at http://www.see-incorp.com . Please feel free to contact me if you have questions. Sincerely, Paul
-- Dr. Paul Martin S.E.E. Incorporated 801 Mahler Road Suite G Burlingame, CA 94010 USA
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Dear Nestor, } } As a subscriber to the ListServer, I would like to express my } appreciationn and support for all your efforts to minimize the SPAM/JUNK } mail and viruses we receive. } } Gill Bond } New Mexico Tech } } On Mon, 26 Apr 1999, Nestor J. Zaluzec wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Ricardo } } } } Your accuser was text in your header. No individual per say. } } Your Email contained information which matched to the automatic filter in the } } listserver. This is something that everyone has to put up with } } to protect the greater good of the ListServer community. There } } are a number of checks and balances that have been put into the } } system over the years to minimize SPAM/JUNK Mail and Viruses. } } Your message got flag as a POTENTIAL source, it was stopped } } after you replied as per the instructions it subsequently was passed } } through to the list after HUMAN review. Yes this caused a delay } } in your posting, however, the number of Undesireable messages } } stopped by the filter is far greater than the few that are delayed. } } If you do not understand or appreciate this I am sorry, but you need } } to realize that thousands of people can be affected by inappropriate } } information in the Email. Maintaining and monitoring postings } } even if it is only by a software program is the appropriate thing } } and prudent job to do in today's environment. } } } } If you wish to submit anyone's name to your lawyers it should be } } me as the ListServer SysOp. I am solely responsible for the installing } } and maintaining the filter as well as operating the server. I have setup the } } key word lists and I edit and maintain them. There are no other } } persons involved. } } } } } } Nestor } } } } } } } } } } } } } } } Hi,
I am amazed and grateful for the filter that Nestor manages. About every day I swear at AOL when I find my personal computer full of obnoxious messages. Why can't AOL be as good as Nestor in firewalling rot? Sincerely, Hildy Crowley
We are in the process of sifting through information on digital TEM = cameras and have been concentrating on systems from Gatan, Advanced = Microscopy Techniques (AMT) and Soft Imaging Systems. I would = appreciate feedback from experienced users on your degree of = satisfaction with any of the camera systems from these companies (i.e. = resolution, acquisition software, customer support, etc.). Thanks for = your help.
Steve
Stephen Fields, Ph.D. Oklahoma Medical Research Foundation 825 N.E. 13th Street Oklahoma City, OK 73104 Office Phone: (405) 271-7245 Fax: (405) 271-3153 steve-fields-at-omrf.ouhsc.edu
} I accidentally ran into part of a Nova show on a PBS station showing } some very interesting micro videos of cells, micro animals, and } parasites, colorized SEMs, and fiber optic close-ups of insects and } caterpillars. The series of 3 shows is called Odyssey of Life. I don't } know if it is current issue Nova, but I think the pictures would } interest many on the list. I missed the 1st show, saw part of the } second. The 3d show is an interview with the maker, the person who } made some fascinating human embryo pictures some years back. } } } Leonard Corwin } Research Chemist } Fort Dodge Animal Health } Princeton, NJ 08543-0400
Leonard -
Here'sthe description of the third tape that appears in the Project MICRO bibliography (URL below):
Nilssen, L. 1996 The Photographer's Secrets 1 hour, $19.95 from WGBH Boston Video, P.O.Box 2284, South Burlington, VT, 05407, 800-255-9424. Lennart Nilssen is famous for his beautiful images of human development. This is part three of the Nova series Odyssey of Life (or part 4 of the series The Wonder of Life) It explains his use of SEM and other imaging tools that blur the line between microscopy and macrophotography; it will be particularly interesting for budding microscopists. All ages.
Caroline Schooley Educational Outreach Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
The Quebec Chapter of the Microscopical Society of Canada is proud t= o announce:
3-Day Workshop covering all aspects of VARIABLE PRESSURE Scanning Electron Microscopy. The guest speaker is Dr. David Joy and he will cover the following topics:
Introduction to Variable Pressure S= EM Beam Interaction with Solids SE electron Imaging BSED Imaging VPSEM Imaging X-ray work in Variable Pressure Mode Lab. Sessions ... Since the number of participants is very limited we strongly advise interested people to act quickly.
DATE: August 24-26 WHERE: Varennes just south-east of Montr=E9al, QC., Canada
WORKSHOP on VARIABLE PRESSURE SEM Guest Speaker : Dr. David C. Joy
For more Information please contact : Dr. Pierre Hovington, (IREQ) ovington-at-ireq.ca , = 450 652-8125 fax: 450 652-8424
Hi there, Nigel Unwin did this in the '70s using a strand of spider silk. He picked up a spider on an aperture (don't know what species, but it was in Cambridge) and dangled it so a strand fell across the centre of the aperture. Then he coated it with gold.
The original reference is in Zeitschrift fur Naturforschung Vol 29 A (1974) pp 158-163 and was also printed as a Philips Applications bulletin EM 95 Dec 1974.
***************************************************** Mel Dickson, Deputy Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Ricardo } } Your accuser was text in your header. No individual per say. } Your Email contained information which matched to the automatic filter in the } listserver. This is something that everyone has to put up with } to protect the greater good of the ListServer community. There } are a number of checks and balances that have been put into the } system over the years to minimize SPAM/JUNK Mail and Viruses. } Your message got flag as a POTENTIAL source, it was stopped } after you replied as per the instructions it subsequently was passed } through to the list after HUMAN review. Yes this caused a delay } in your posting, however, the number of Undesireable messages } stopped by the filter is far greater than the few that are delayed. } If you do not understand or appreciate this I am sorry, but you need } to realize that thousands of people can be affected by inappropriate } information in the Email. Maintaining and monitoring postings } even if it is only by a software program is the appropriate thing } and prudent job to do in today's environment. } } If you wish to submit anyone's name to your lawyers it should be } me as the ListServer SysOp. I am solely responsible for the installing } and maintaining the filter as well as operating the server. I have setup the } key word lists and I edit and maintain them. There are no other } persons involved. } } } Nestor
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
In the last 2 1/2 years, I have been working with muscle tissue and blood buffy coats from dogs infected with Hepatozoonosis. Part of my PhD study is to morphologically detail the asexual stages of the parasite, Hepatozoon americanum which is responsible for canine hepatozoonosis in North America. Based on the literature, other researchers believe the parasite lives in neutrophils; however based on preliminary TEM work, I am certain these parasite dwell in a "transformed" or " altered" macrophage. I am aware that normal macrophages and neutrophils can be easily differentiated based on there ultrastructural characteristics; however, since these cells are transformed due to the parasite's existence in the cytoplasm, the basic characteristics of the cell have changed; therefore, I am interested in finding a method which will positively identify the cell in question. Currently with light microscopy , I have tried with no success immunohistochemistry and enzyme histochemistry. Thus, my question is: Does anyone have a working TEM marker or TEM method for positively differentiating dog macrophages from neutrophils other than by their basis ultrastructural characteristics? I would appreciate any comments or suggestions.
Dear Listservers, Does anyone have a reference or simple method for quantifying viruses in solution? A colleague has a suspension which needs to be quantified. One assay technique detects a concentration of 10 (E10) per ml while a second assay technique detects a concentration of 10 (E11) per ml. I wonder if the error involved with quantification by EM would be too great to shed any light on the problem. I expect the best option would involve negative staining. Thankyou.
John Brealey Queen Elizabeth Hospital EM Unit Adelaide South Australia }
We have just installed a CM200 FEG TEM/STEM and EDS.
Does anyone know what are the best conditions (Gun lens/extraction voltage/spot sizes/C1 and C2 aperture sizes etc etc) for performing high spatial resolution microanalysis?
We are trying to look at chemically heterogenous particles which are only a few nanometres in size {smaller} . {/smaller}
I am looking for an inexpensive microscope 5X to 100 X and would probably settle for a monocular. Changing oculars is not a problem.
It is for photography. I have a 4X5 Leintz camera and a 35mm Ziess with a front reflex attachment so a vertical ocular would be ideal.
It is for personal use so the cosmetics are not very important. The optics are important. I have a pretty good range of lighting equipment so lights are not necessary.
If you have any thing that might be of interest to me please email me. I am looking to spend less than $250.00.
I realize that I probably won't be able to get everything I want in one scope.
thanks Gordon
Gordon Couger gcouger-at-couger.com www.couger.com/gcouger Stillwater, OK 405 624-2855 GMT -6:00
-----Original Message----- } From: Stan, Pat, Robert, Peter, and Angus Hansen [mailto:sehansen-at-bellatlantic.net] Sent: Monday, April 26, 1999 8:10 PM To: rschoonh-at-sph.unc.edu Cc: COMPMED-at-LISTSERV.AALAS.ORG; histonet; Microscopy ListServ
Anyone have a surplus Reichert/Leica FC4E or parts? ..or know of one available? I would like to find some back up parts and equipment and sources. Also are there othere cryo-ultramicrotomes for TEM uses other than RMC, Microstar and Leica? Also accessories? Also are tere any ultra-cryo type list servers? Jeff Day/ 'JD'
___________________________________________________________________ You don't need to buy Internet access to use free Internet e-mail. Get completely free e-mail from Juno at http://www.juno.com/getjuno.html or call Juno at (800) 654-JUNO [654-5866]
John: Mix 1 drop of virus solution and 1 drop of a suitable size latex solution of known and reasonably similar concentration as the unknown. The latex spheres concentrations are fairly accurately known in terms of percent w/v. This, for a certain size latex spheres can be calculated and diluted to achieve the required particle number concentration.
If greater accuracy is required the concentration of the latex stock solution can be checked in one of the electronic particle sizing/ counting instruments (eg. Coulter counter). Coated grids are applied to the mixed drop and in the case of small particles, this is negatively stained.
Disclaimer: ProSciTech provides the largest range of latex particles in small volumes (mostly 5ml) from 0.1um to 20um in 12 sizes. Applications and concentration versus size conversions and other data is online, linked to page S2, which is accessed from "Contents". Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht } ml } Quantification of viruses in solution. } } Dear Listservers, Does anyone have a reference or simple } method for } quantifying viruses in solution? A colleague has a } suspension which needs } to be quantified. One assay technique detects a } concentration of 10 } (E10) per ml while a second assay technique detects a } concentration of 10 } (E11) per ml. I wonder if the error involved with } quantification by } EM would be too great to shed any light on the problem. I } expect the best } option would involve negative staining. Thankyou. } } John Brealey } Queen Elizabeth Hospital } EM Unit Adelaide South Australia } } } }
Hi, There were a debate on the list a few month ago about anti-vibration table (home made systems and commercial ones). If anybody has collected the emails related to that, could he forward them directly to me. Thanks for your help! Cecile Dr. Cecile Prouteau School of Metallurgy & Materials The University of Birmingham Edgbaston, Birmingham B15 2TT UK E-mail : c.prouteau-at-bham.ac.uk tel : (UK=44)- (0)121 414 5170 fax : (UK=44)- (0)121 414 5232
Question: I am a relative novice at microscopy and have the task of trying to freeze-substitute samples containing large amounts of fat in the form of fat droplets (size range between sub-micron to approx. 10 microns) for TEM. Although I can produce reasonable results for semi-thin sections for LM, the fixatives (combinations of glutaraldehyde, OsO4, RuO4, and UA) and solvents (acetone and methanol) I have used do not fix the fat sufficiently for ultra-thin sectioning. (I have also varied the substitution and fixation periods from two days to over a week with little success). Can anybody suggest a fixative/solvent regime which will fix and dehydrate, but not mobilize, the fat? I have good indications that methanol and ethanol are redistributing the fat considerably at -90C, even after three days osmication. Sample preparation also requires the temperature to remain below -25C until polymerization. Any suggestions would be welcome! Regards, Nick Johnson
Nick.B.Johnson-at-Unilever.com
Dr. N. Johnson, Structural Analysis, Unilever Research, Colworth House, Beds, U.K.
We have a Hitachi S-520 SEM installed in 1983. Lately, we have experienced instability problems in the SEM and I'm not sure what to do. The Hitachi S-520 SEM has a single rotary pump, single diffusion pump and Pirani gauge. On initial pumping and warm-up, the SEM gets down to high vacuum (reading 10uA) quite normally in about 20min. On applying HT (20kV), everything appears normal for approximately 80min, then, suddenly the HT switches itself off and the vacuum gauge rises to 15uA in 30sec and then falls back to10uA in 2min. If the slightest gun emission current is applied when the SEM says it's ready, the HT instantly switches itself off. I have cleaned the filament assembly, anode and gun chamber around the anode. However, I have not cleaned the insulator at the top of the gun chamber. The Pirani gauge is clean and the diffusion pump is not too hot. Any advice would be much appreciated and gratefully received. Thankyou.
John Brealey Queen Elizabeth Hospital EM Unit Adelaide South Australia
Steve, We have two AMT systems with 2K x 2K Kodak Megaplus cameras coupled to a Kodak XLS8600 printer and a Codonics (Kodak) NP1600 printer. We just upgraded to Windows NT with a few minor glitches which for the most part have been resolved. We have been very satisifed with the results as have our customers. The support is very good as is the reliability. I have no experience with the Gatan or SIS cameras. Russ, Xerox
-----Original Message----- } From: Steve Fields [mailto:steve-fields-at-omrf.ouhsc.edu] Sent: Monday, April 26, 1999 3:42 PM To: 'Microscopy-at-MSA.Microscopy.Com'
We are in the process of sifting through information on digital TEM cameras and have been concentrating on systems from Gatan, Advanced Microscopy Techniques (AMT) and Soft Imaging Systems. I would appreciate feedback from experienced users on your degree of satisfaction with any of the camera systems from these companies (i.e. resolution, acquisition software, customer support, etc.). Thanks for your help.
Steve
Stephen Fields, Ph.D. Oklahoma Medical Research Foundation 825 N.E. 13th Street Oklahoma City, OK 73104 Office Phone: (405) 271-7245 Fax: (405) 271-3153 steve-fields-at-omrf.ouhsc.edu
Try the May '97 Microscopy Today, pg. 20 (Microscopy 101):
Quantitation of Virus Particles by Negative Staining for TEM by Robert Alain, Institut Armand-Frapplier
Phil
} } Quantification of viruses in solution. } } Dear Listservers, Does anyone have a reference or simple method for } quantifying viruses in solution? A colleague has a suspension which needs } to be quantified. One assay technique detects a concentration of 10 } (E10) per ml while a second assay technique detects a concentration of 10 } (E11) per ml. I wonder if the error involved with quantification by } EM would be too great to shed any light on the problem. I expect the best } option would involve negative staining. Thankyou. } } John Brealey } Queen Elizabeth Hospital } EM Unit Adelaide South Australia } }
****be famous! send in a tech tip or question*** Philip Oshel Technical Editor, Microscopy Today PO Box 620068 Middleton, WI 53562 Voice: (608) 833-2885 Fax: (608) 836-1969 (please make sure my name is on any fax) oshel-at-terracom.net
I am submitting this for the person above. Please reply to her.
} I have a question concerning the measurement of the hyphe of the fungi } Aspergillus oryzae in a agar matrix. } We have grown the fungi in a agarmatrix, then we make thin coupes and put them } under the microscope. then we want to measure the amount of fungi in a certain } volume of agarmatrix. We make pictures of the coupes and try to work on it } with the computer programe 'Adobe Photoshop'. The problem we encounter is that } the colour inside the hyphe is the same as the colour of the agarmatrix, the } only thing we do see is the wall of the hyphe. So we would like to colour the } inside of the hyphe, so we can differentiate between inside and outside the } hyphe. Using fluorchromen to colour the inside gives us the problem that it } only colours certain parts (for instance living or dead) of the hyphe. } -So i am looking for a dye that can colour all inside of the hyphe if it is } possible. } -Or a dye that colours the cell wall very clearly } -Or a stain that colours the agarmatrix without colouring the fungi. } } Thank you very much for any tips you might have } } greetings Marjolein } } } } Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
This is a good job for a grad student / post doc. You should run through the calculations of probe size vs Cs & Cc. Then do the physical measurments of probe size vs beam current and create yourself a operational / working curves. The expt. measurements are always slightly different than the theoretical, but the calculations will put you in the right general area.
We did this for the VG here at ANL, you can see the results in the AAEM Electronic Notebook, and/or look at the Proc. of Microscopy & Microanalysis '98 Atlanta pge 386.
If you go to the notebook (http://tpm.amc.anl.gov/NJZ/AAEMNoteBook.html) do a search for the key words: FEG, brightness, VOA Current
Nestor
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sorry for the flood but you asked for it. The followin e-mails contain the most recent discussions via e-mail as I am a year behind in the Tips & Tricks archives. There is also another discussion archived at the Tips & Tricks site you may be interested in. Point your browser to
http://www.biotech.ufl.edu/~emcl
follow the Tips & Tricks link and look in the Light Microscopy section.
Our whole site is under renovation so please bear with me for the next month or so while I get it finished. Comments, suggestions, and contributions are always welcome.
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { GO GATORS Scott D. Whittaker 218 Carr Hall EM Technician Gainesville, FL 32610 University Of Florida ph 352-392-1184 ICBR EM Core Lab fax 352-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ The home of " Tips & Tricks "
The New England Society for Microscopy will hold its Sixteenth Annual Spring Symposium at Marine Biological Laboratories, Woods Hole, Massachusetts on May 7 & 8, 1999. In addition to our scientific presentations, this year's Symposium=
will feature PROEJCT MICRO, the new Great Explorations in Math and Scienc= e Program designed to introduce Microscopy to elementary- and middle school-aged students. If you are interested in participating in this exciting new program, be sure to register and join us for dinner on Frida= y evening. =
PRIZES for Best Poster, Best Student Poster, and Best Photo-As-Art will also be awarded. If you're interested in submitting your poster for judging, please contact Dr. Changmo Sung at Changmo_Sung-at-uml.edu. =
PROGRAM Friday, May 7th
12:00 pm Registration: Swope Center
1:00 pm Welcome: Lillie Auditorium
Session I Chairperson: Doug Taatjes, UVM
1:05 pm Laser Scanning Cytometry as an Imaging System Ed Luther, Senior Scientist - Biology, CompuCyte Corporation, Cambridge, MA
1:45 pm Focused Ion Beam Microscopy: The New Kid on the Block Dr. David Casey, Senior Scientist, Micrion Corp, Peabody, MA
2:25 pm Membrane Fusion Machinery in Cells Dr. Bhanu Jena, Dept. of Surgery and Biomedical Eng., Yale University, New Haven, CT
3:05 pm Afternoon Break Coffee served in Lillie 103
Session II Chairperson: Tony Garratt-Reed, M.I.T.
3:20 pm Multi-technique Characterization of Emissive Coatings on Electrod= es Dr. Chris Peters, Senior Project Engineer R & D, Osram Sylvania, Beverly, MA
4:00 pm Microscopic Approach to the Study of Pancreatic Islets Dr. Thomas Jetton, Ergo Scientific, Inc., Cambridge, MA
4:40 pm Advances in Microanalysis: A Look at the Past, Present and Future=
Dr. William Hardy, CEO, Princeton Gamma Tech, Princeton, NJ
5:30 pm Cocktails and Dinner: Swope Center
7:30 pm Project Micro "Festival" Meigs Room, Swope Center Dr. Burton E. Goodrich, Jr., Executive Director, South Coast Educational Collaborative Janet E. Goodrich, Principal, Miscoe Hill Elementary School, Mendon, MA
Saturday, May 8th
7 to 8:00 am Breakfast: Swope Center
Session III Chairperson: Eben Oldmixon, HSPH =
8:30 am The Use of Electron Microscopy in the Characterization of Carbon Deposits Paul Anderson, Chemistry Department, Northeastern University, Boston, MA
9:10 am Confocal Microscopy of Nuclear Envelope Breakdown Dr. Mark Terasaki, Asst. Prof., Dept. of Physiology, UCONN Health=
Ctr., Farmington, CT
10:00 am Commercial Exhibits and Posters: Swope Center Coffee and doughnuts will be served
12:30 pm Presentation of Poster and Photos-As-Art Awards and Door Prizes: =
Poster Area, Swope Center
1:00 pm Lunch with Short Tour of MBL: Swope Center =
2:00 pm 2-hour Discovery Cruise aboard the R/V Patriot II Tickets must be reserved in advance
Register Today! Contact L. Kirstein at NESM-at-compuserve.com. Registratio= n Deadline will be extended to April 30th. =
SILENT AUCTION, OCEANOGRAPHY CRUISE, POSTER AWARDS, DOOR PRIZES =
I am looking for a CCD camera and image capture card for use with a standard Zeiss light microscope. I've been looking all over and I can't find any cameras designed for this use. Can anyone help me out with the name of a vendor or a website?
I recall reading somewhere a long time ago about special fixation procedures for non-conductive samples that are hard to adequately coat. I seem to remember tannic acid being involved. Does this ring a bell with anybody out there?
Specifically, we are processing pig intestine samples for SEM and are having problems with charging on the microvilli. We have repeatedly coated the samples with Au/Pd, but the problems persist. Perhaps there's a specialized processing technique that can help?
Thanks in advance. Randy
Randy Tindall Electron Microscope Core College of Veterinary Medicine University of Missouri - Columbia Phone: 573-882-8304
Dear Mel, } } Does anyone know what are the best conditions (Gun lens/extraction } voltage/spot sizes/C1 and C2 aperture sizes etc etc) for performing high } spatial resolution microanalysis? } You want to minimize both x-rays generated up-column (primarily at the condenser apertures) and those generated by scattered, possibly multiply scattered, electrons interacting in the specimen and detector areas. The former will depend on the the composition of the apertures, and is lessened by the use of low-z materials. We installed an aluminum C1 aperture for this reason, and we obtained some ~1 mm thick Be disks with 100 um holes for use as C2 apertures. The aluminum C1 works very well, but the Be C2 has charging problems from the surface oxide. Our best results for spatial resolution were not spectacular, but since the high-voltage scope is used for thick specimens where the beam will spread within the specimen, we were not too concerned. You want to have a high enough voltage that beam spread and multiple scattering within the spe- cimen are minimized, but you need to consider the efficiency of your shielding as a function of voltage. I suggest using calculations to arrive at a proposed optimum and experimenting with the relevant parameters to find the true optimum. } } We are trying to look at chemically heterogenous particles which are only } a few nanometres in size. } Will you be able to get sufficient signal from one of these so that the background produced by excitation of the rest of the specimen by stray radiation is small in comparison? Do you want to characterize the hetero- geneity within a single particle? Good luck. Yours, Bill Tivol
Thanks to you all, It's been great help Cecile Dr. Cecile Prouteau School of Metallurgy & Materials The University of Birmingham Edgbaston, Birmingham B15 2TT UK E-mail : c.prouteau-at-bham.ac.uk tel : (UK=44)- (0)121 414 5170 fax : (UK=44)- (0)121 414 5232
Dear Listpeople: Is it possible to cut reasonable quality longitudinal sections of rat molar undecalcified tooth between 100 nm to 0.1 mm with a diamond or sapphire knife? Does it perhaps require special circumstances and equipment? They will be used in immunocytochemical labeling for TEM. Thank you, Lauri
Dr. Lauri J. Pelliniemi, M.D. Telephone +358-2-3337312 University of Turku Kiinamyllynkatu 10 Internet e-mail lauri.pelliniemi-at-utu.fi FIN-20520 Turku FINLAND. Europe http://www.utu.fi/med/em/personne.html
Dear Mel, When you do EDS on STEM, you use the same sort of conditions as an SEM. Small aperature and higher condensor lens setting will give you better spacial resolution, but at the cost of x-ray count rate. On a thin sample you may only have a hundred counts per second or less, so count times may get long You wrote: } } We have just installed a CM200 FEG TEM/STEM and EDS. } } Does anyone know what are the best conditions (Gun lens/extraction voltage/spot sizes/C1 and C2 aperture sizes etc etc) for performing high spatial resolution microanalysis? } } We are trying to look at chemically heterogenous particles which are only a few nanometres in size. } ***************************************************** } Mel Dickson, } Deputy Director. } Electron Microscope Unit, } University of New South Wales. } Sydney NSW 2052 Australia } } Phone (+612) 9385-6383 } Fax (+612) 9385-6400 } Website {http://srv.emunit.unsw.edu.au} } ***************************************************** Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
} } } "Bartlett, Jeanine" {jqb7-at-cdc.gov} 04/27 6:41 AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
As did I!
-----Original Message----- } From: Stan, Pat, Robert, Peter, and Angus Hansen [mailto:sehansen-at-bellatlantic.net] Sent: Monday, April 26, 1999 8:10 PM To: rschoonh-at-sph.unc.edu Cc: COMPMED-at-LISTSERV.AALAS.ORG; histonet; Microscopy ListServ
On Tue, 27 Apr 1999, Chris Bradley wrote:
} I am looking for a CCD camera and image capture card for use with a standard Zeiss light } microscope. I've been looking all over and I can't find any cameras designed for this } use. Can anyone help me out with the name of a vendor or a website?
???? Pretty much SOP today and all over the Web.*. Check Edmunds or McCrone. Also, any C-mount camera can easily be adapted to your microscope tube. The grabber cards are ubiquitous as well.
However, let me suggest you start the other way 'round. Define your application needs and decide on the software first. Then, choose grabber and camera from the supported list. Otherwise, you may buy something which won't give you what you need.
Method 1: To determine virus in solution is to do a plaque assay. Keep = diluting viral stock until there is approxamitely 1 virus per ml and = infect cell cultures with it. This determine infective particles.
Method 2: Ref. is Sharp, D.G., 1974. Proceedings of 32nd Annual = Meeting of EMSA. Clayton's Publishing Division, p. 264-265.=20
Method 3: McCombs, R.M., Benyesh-Melnick, M. and Brunschwig, J.P. 1966 = Biophysical studies of vesicular stomatitis virus. J. Bacteriol. = 91:803-812
The suggestion of using latex spheres is ok but has one major flaw in the = technique, Do the sphere's and viral particles stick to the grid in equal = proportions????? probably not.
In my experence, I am able to dectect down to 1 x 10 e6 particles per ml. = using method 2 above.
Best of luck,
Ed
Edward P. Calomeni Dept Pathology - EM Lab 3000 Arlington Ave. Toledo, OH 43614
} } } "Nestor J. Zaluzec" {zaluzec-at-Sparc5.Microscopy.Com} 04/26 9:38 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html=20=
Dear Listservers, Does anyone have a reference or simple method for quantifying viruses in solution? A colleague has a suspension which needs to be quantified. One assay technique detects a concentration of 10 (E10) per ml while a second assay technique detects a concentration of 10 (E11) per ml. I wonder if the error involved with quantification by EM would be too great to shed any light on the problem. I expect the best option would involve negative staining. Thankyou.
John Brealey Queen Elizabeth Hospital EM Unit Adelaide South Australia }
Randy, check this reference: Murakami, T (1973) A revised tanin-osmium = method for non coated sem specimens. Arch. Hist. Jap 36(3) 189-. Or = what I think is superior, the OTOTO method of Robert Kelley which uses = thiocarbohydrazide as a ligand to bind more osmium to the surface. = Hayat has this technique in one of his SEM method volumes and Kelley's = original citation is in Ultrastructure Research 45 (1973) 254-258.
Hank Adams Integrated Microscopy Core Cell Biology Baylor College of Medicine
Nick: I am more of a novice than you, and would GREATLY appreciate any tips you get on this. ( In fact would any *experienced* lab like to do this work for me for a fee?) I am doing something similar, a suspension of 15 micron liposome aggregates containing vegetable oils.
I don't have the answer. I presume osmication or rutheniation should be done extensively at room temp or preferably *above* (37C), before dehydration. A method using imidazole was highly recommended to me, as better than extended osmication, see reference below. I do expect that freeze-substitution is the way to go for dehydration. Acetone is known to extract fewer phospholipids than do the alcohols; it may be better for nonpolar lipids as well. Just keep it well-chilled.
Angermuller S, Fahimi HD, Histochem J 1982 Sep;14(5):823-35, Imidazole-buffered osmium tetroxide: an excellent stain for visualization of lipids in transmission electron microscopy. (nb: in PubMed just now I hit the "related articles" button and found a few interesting articles http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&uid=6182131&dopt=m&dispmax=100 )
I hired a local EM tech to work on this and was disappointed with the results, so hope you do find a method that works, and that you can let me know. For your smaller particles, presumably you can use cryo TEM with an energy loss filter and avoid fixing entirely, but I can't with my large aggregates
Good luck Richard
} } } Nick B Johnson {Nick.B.Johnson-at-unilever.com} 04/27/99 05:13am } } } Question: I am a relative novice at microscopy and have the task of trying to freeze-substitute samples containing large amounts of fat in the form of fat droplets (size range between sub-micron to approx. 10 microns) for TEM. Although I can produce reasonable results for semi-thin sections for LM, the fixatives (combinations of glutaraldehyde, OsO4, RuO4, and UA) and solvents (acetone and methanol) I have used do not fix the fat sufficiently for ultra-thin sectioning. (I have also varied the substitution and fixation periods from two days to over a week with little success). Can anybody suggest a fixative/solvent regime which will fix and dehydrate, but not mobilize, the fat? I have good indications that methanol and ethanol are redistributing the fat considerably at -90C, even after three days osmication. Sample preparation also requires the temperature to remain below -25C until polymerization. Any suggestions would be welcome! Regards, Nick Johnson
Nick.B.Johnson-at-Unilever.com
Dr. N. Johnson, Structural Analysis, Unilever Research, Colworth House, Beds, U.K.
We had AMT install their 12-bit system that uses a Hammamatsu camera. I'm using a Lexmark laser printer for everyday prints. but have access to a multitude of other printers on our network. Though we have no dye-sub printer, excellent results have been achieved with premium inkjet photo paper and inkjet printers.
AMT's reputation among vendors and users, performance of instrumentation, and price were all major considerations. Subsequent interaction after installation has been very good, too. One other reason for going to AMT was the non-interest by GATAN.
Overall, the system has more than lived up to expectations. I don't miss the darkroom one bit.
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Steve, We have two AMT systems with 2K x 2K Kodak Megaplus cameras coupled to a Kodak XLS8600 printer and a Codonics (Kodak) NP1600 printer. We just upgraded to Windows NT with a few minor glitches which for the most part have been resolved. We have been very satisifed with the results as have our customers. The support is very good as is the reliability. I have no experience with the Gatan or SIS cameras. Russ, Xerox
-----Original Message----- } From: Steve Fields [mailto:steve-fields-at-omrf.ouhsc.edu] Sent: Monday, April 26, 1999 3:42 PM To: 'Microscopy-at-MSA.Microscopy.Com'
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We are in the process of sifting through information on digital TEM cameras and have been concentrating on systems from Gatan, Advanced Microscopy Techniques (AMT) and Soft Imaging Systems. I would appreciate feedback from experienced users on your degree of satisfaction with any of the camera systems from these companies (i.e. resolution, acquisition software, customer support, etc.). Thanks for your help.
Steve
Stephen Fields, Ph.D. Oklahoma Medical Research Foundation 825 N.E. 13th Street Oklahoma City, OK 73104 Office Phone: (405) 271-7245 Fax: (405) 271-3153 steve-fields-at-omrf.ouhsc.edu
id PAA24629; Tue, 27 Apr 1999 15:19:40 -0500 (EST) Message-Id: {3.0.3.32.19990427162046.006fc9bc-at-biotech} X-Sender: gwe-at-biotech X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.3 (32)
Regarding TEM of fat, I might suggest two possible approaches. One would be to use a hydrophobic resin like Lowicryl HM-20 or HM-11. It might even serve as the substitution media. Another thought is to freeze dry the samples and embedd them in resin directly . One could also expose the sample, after drying, to osmium vapors from the crystals in order to keep things anhydrous. I would recommend a resin that can tolerate a little water, like Epon or its equivalent, or a methacrylate based resin.
Would using freeze-fracture EM give the results you are after??
Fats and lipids are a nightmare for EM.
Greg Erdos Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
Has anyone out there managed to add transmitted-light illumination to the optical microscope of a JEOL 840? I'd appreciate hearing from you.
thanks
Ritchie
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
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I have located two good prospects for CCD cameras which do not need to be tethered to a computer. They are:
Olympus DP10 - uses a smart card to record images. Has a built in LCD panel for formatting your image and a keypad for interacting with the programming. You can print directly from the camera if desired. Resolution: 1280x1024. Price ~$3000
Fugi HC-300Z: does not have built in LCD panel so it is helpful to attach a small monitor for setting up your formatting, etc. Downloads images directly to a ZIP disk. Has small keypad for interfacing with software. Resolution: 1280 x 1000. Price ~$4000
Both mount using standard C-mounts. They may need a relay lens. They can be used on a copy stand with a standard camera lens.
Kodak also makes a microscope tube adapter for use with one of their consumer cameras. Does not appear to give the quality that the above ones do and is tethered to a computer, as are SPOT cameras, etc. Also, most of the other available cameras are substantially more expensive ( most are "cooled") although they may perform better under low light.
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
Chris Bradley wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi everyone, I am looking for information about selected area electron channelling technique which is used for strain measurement of crystalline materials. I have read few articles but I do not have any idea about what size sample should be prepared, what kind of backscatter dedector do i need etc. If anybody who has experience with that technique please inform me. Thanks
} Olympus DP10 - uses a smart card to record images. Has a built in LCD } panel for formatting your image and a keypad for interacting with the } programming. You can print directly from the camera if desired. Resolution: } 1280x1024. Price ~$3000
If it records on SmartMedia, one does not need a frame grabber with it. I use a Lexar SmartMedia (parallel-port) reader. It functions like a removeable disc drive permitting one to read/write the SM. It cost me $37.
} Fugi HC-300Z: does not have built in LCD panel so it is helpful to attach } a small monitor for setting up your formatting, etc. Downloads images } directly to a ZIP disk. Has small keypad for interfacing with software. } Resolution: 1280 x 1000. Price ~$4000
Again. No grabber.
} Kodak also makes a microscope tube adapter for use with one of their } consumer cameras.
Also, it (DC-120) retains its non-removeable lens and has poorer resolution.
Consider also the Pixera and the MicroVision in this class of camera.
What all of these do NOT permit is the use of real-time image processing software. All processing is done post hoc. If this is OK in one's application, fine. Otherwise, one must consider a separate camera + grabber.
How are you coating your specimens? The common approach is to place the specimen in the chamber and to let the coater get on with the task? Microvilli are difficult to coat if you use this method and if this is followed by the common observation at 20kV then I can understand why the samples charge.
Try this coating method.
1) Set the sample at approx 45deg in the coater with a working distance of 5cms 2) With a coater that indicates kV on its variable control set this = at the lowest level at which a plasma will strike (probably ~800 volts) and then tune for 20mA. If the coater has a deposition control set it at 10m= A. 3) Coat for one minute 4) Tilt the sample in exactly the opposite direction and repeat.
Try it out at 10kV
5) If not quite good enough - run the coater to obtain the best vacu= um that you can and then try to strike a plasma without gas being introduced= , or at worst the very minimum of gas - coat for one minute with the sample=
flat.
To improve your microscopes performance at the lower kV be sure to have a= t least 100uA emission current with a Japanese instrument (W) with Philips=
50uA(W) and a WD of less than 10mm. If you can not obtain these curren= t levels you need to place the filament nearer the cap.
Hope this helps?
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
Sorry to hear you are having problems but I am afraid high voltage is wha= t we call in the trade a "standard fault" on the otherwise superb S520.
Not being on the spot it is of course very difficult. Any fall off in vacuum level is likely to trip the high voltage, however I am inclined to=
tell you to look at the gun area. Inside the gun casing are the connections between the gun components and the high voltage cable. These=
connections in time break down and give a wide variety of problems; this = is 90% likely to be the problem! I do not recall many vacuum funnies on 520= s.
Just as a test, run the instrument up at a lower kV (say 2 or 5kV) and s= ee if the fault is identical. If the fault changes we prove that the fault = is high voltage related, then you go to the area I have outlined.
This area of the gun is sealed, but if that is where the problem is you have three routes (1) have a go yourself when we can give you step by ste= p instructions. (2) Get the local agent to sort it out. (3) Look around t= o see if there is a company nearby that specialises in high voltage repairs=
Well, I hope we have helped, remember a motto "if a man built it a man ca= n fix it", no offence to the ladies as man is used in the generic sense.
Steve Chapman
Senior Consultant E.M. Protrain, 16 Hedgerley, Chinnor, Oxford OX9 4TN, England. Tel & Fax 44 (0)1844 353161 Web Site - http://ourworld.compuserve.com/homepages/protrain For Consultancy and Courses in Electron Microscopy World Wide
We routinely use a variant of Mallick & Wilsons ( IITRI/SEM 1975; 259-264) OTOTO (osmium -thiocarbohydrazide) technique for cochlea specimens (organ of Corti - inner ear) which have a very convoluted shape and are extremely difficult to sputter coat. The wash must be thorough, including the neck, rim and cap of the specimen vial; any trace of TCH or OsO4 remaining will form a precipitate when the other reagent is added. We have also successfully used the osmium-tannic acid method but prefer the former for very convoluted specimens. One advantage of this method over sputter coating is that it permits subsequent redissection and SEM observation without recoating and/or embedding for TEM examination (e.g., Hunter-Duvar IM, Mount RJ (1978). The organ of Corti following ototoxic antibiotic treatment. Scanning Electron Micros/1978/II., 423-430).
O-T-O-T-O SUMMARY
- fix, postfix OsO4 - 1% aq TCH 20 min. with agitation - wash well - 1% aq OsO4 2 hr. with agitation - wash well - 1% aq TCH 20 min. with agitation - wash well - 1% aq OsO4 2 hr. with agitation - wash well - dehydrate - CPD
"Tindall, Randy D." wrote:
} I recall reading somewhere a long time ago about special fixation procedures } for non-conductive samples that are hard to adequately coat. I seem to } remember tannic acid being involved. Does this ring a bell with anybody out } there? } } Specifically, we are processing pig intestine samples for SEM and are having } problems with charging on the microvilli. We have repeatedly coated the } samples with Au/Pd, but the problems persist. Perhaps there's a specialized } processing technique that can help?
--
Richard J. Mount Auditory Science Laboratory, Department of Otolaryngology & Brain and Behaviour Division/Research Institute The Hospital for Sick Children Toronto, Ontario, Canada (416) 813-6551; Fax (416) 813-8456 http://www.sickkids.on.ca/HSCWeb/Otolaryngology/Otoalias/Earhome1.htm
Hello John: } "The suggestion of using latex spheres is ok but has one } major flaw in the technique, Do the sphere's and viral } particles stick to the grid in equal proportions????? } probably not."
Maybe you are right, but I doubt it, especially for small particles like virus. When touching a coated grid repeatedly against a drop of virus solution and part blotting it, the aim is to retain a thin film of the aqueous solution. This dries down and the particles adhere. This is my "model" for just applying an aqueous solution. I would expect that the loss of particles, latex or virus would be small and similar.
When negatively staining particles, those particles are mixed in a metallic solution. After that solution has dried down the particles are actually embedded in the metallic film. Differential retention of particles? . . . . I doubt it very much. It should also be noted that latex spheres have been used for decades to estimate/count particles in EM. Maybe all that data was wrong, but it seems rather more likely that some of those authors did their home work.
I have no hard data on this, but intuitively, with many years of negative staining experience I would be most surprised if this was otherwise. Particle retention during negative staining seems such an obvious problem, somebody is sure to have studied it. Please tell me if I am wrong. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Wednesday, April 28, 1999 4:11 AM, Ed Calomeni [SMTP:ecalomeni-at-mco.edu] wrote:
} } Hi John, } } Method 1: To determine virus in solution is to do a plaque } assay. Keep diluting viral stock until there is } approxamitely 1 virus per ml and infect cell cultures } with it. This determine infective particles. } } Method 2: Ref. is Sharp, D.G., 1974. Proceedings of } 32nd Annual Meeting of EMSA. Clayton's Publishing } Division, p. 264-265. } } Method 3: McCombs, R.M., Benyesh-Melnick, M. and } Brunschwig, J.P. 1966 Biophysical studies of vesicular } stomatitis virus. J. Bacteriol. 91:803-812 } } The suggestion of using latex spheres is ok but has one } major flaw in the technique, Do the sphere's and viral } particles stick to the grid in equal proportions????? } probably not. } } In my experence, I am able to dectect down to 1 x 10 e6 } particles per ml. using method 2 above. } } Best of luck, } } Ed } } Edward P. Calomeni } Dept Pathology - EM Lab } 3000 Arlington Ave. } Toledo, OH 43614 } } 419-383-3484 } 419-383-3066 (fax) } ecalomeni-at-mco.edu } } Edward P. Calomeni } Dept Pathology - EM Lab } 3000 Arlington Ave. } Toledo, OH 43614 } } 419-383-3484 } 419-383-3066 (fax) } ecalomeni-at-mco.edu } } } } } "Nestor J. Zaluzec" {zaluzec-at-Sparc5.Microscopy.Com} } } } } 04/26 9:38 PM } } } } } Quantification of viruses in solution. } } Dear Listservers, Does anyone have a reference or simple } method for } quantifying viruses in solution? A colleague has a } suspension which needs } to be quantified. One assay technique detects a } concentration of 10 } (E10) per ml while a second assay technique detects a } concentration of 10 } (E11) per ml. I wonder if the error involved with } quantification by } EM would be too great to shed any light on the problem. I } expect the best } option would involve negative staining. Thankyou. } } John Brealey } Queen Elizabeth Hospital } EM Unit Adelaide South Australia } } } } } }
Hello Ritchie: Some years ago I too was pushed about by some geologists who could not adapt to a perfectly good atomic number (backscattered) image. I know that compromise transmitted light scopes exist on some WD probes. The 840 was not designed for it. To be useful you require double polarisers; there is insufficient room near the centre of the stage, you require interlocks (no light with SE detector on), the carbon coating kills polarised images. The more you look at the possibility the more problems you will find. If you persist you eventually obtain crummy light microscope images. Non microprobe specialist geologist users often mistake the provided scope (on WD systems) as a weak attempt to provide a light microscope to view the specimen - not so. Its real function is to locate and focus the beam spot using a fluorescent mineral and to calibrate working distance through the low depths of field of the light scope. Use BS to find your way around the specimen. At times that is difficult, but marking slides with India ink and some rough drawings can overcome that. Better still, if available make digital images of your areas of interest and display those on a monitor when locating spots for analyses. There are several ways of "killing that particular cat", installing a transmitted light scope is rather grizzly. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Phone +61 7 4774 0370 Fax: +61 7 4789 2313 Great microscopy catalogue, 500 Links, MSDS, User Notes ********************** www.proscitech.com.au *****
On Wednesday, April 28, 1999 6:18 PM, Ritchie Sims [SMTP:r.sims-at-auckland.ac.nz] wrote: } } } Hello, All } } Has anyone out there managed to add transmitted-light } illumination to } the optical microscope of a JEOL 840? } I'd appreciate hearing from you. } } thanks } } Ritchie } } } Ritchie Sims Phone : 64 9 3737599 ext 7713 } } Department of Geology Fax : 64 9 3737435 } The University of Auckland email : } r.sims-at-auckland.ac.nz } Private Bag 92019 } Auckland } New Zealand }
For an exhaustive review, visit "Curtin's Short Courses in Digital Photography": http://www.shortcourses.com/index.htm
In particular, view Curtin's "Book1: A Short Course in Digital Photography", with emphasis on Chapter 2: The Foundations of Digital Imaging (http://www.shortcourses.com/chapter02.htm) and Chapter 3: Digital Cameras (http://www.shortcourses.com/chapter03.htm)
Another excellent review/tutorial can be found at Sound Vision Incorporated's, "How Digital Cameras Work" (http://www.soundvisioninc.com/howdcw.htm) which deals with the technical nitty gritty and recommendations of camera types based on application needs.
Happy reading and hope this helps.
Best regards,
Walt Bobrowski Subcellular Pathology Parke-Davis Research 2800 Plymouth Road Ann Arbor, MI 48105
The important issue for any microscope is the availability of a phototube with C-mount from Zeiss which is used to attach the camera to the microscope. Any CCD with a C-mount should be OK.
As for image capture cards, there are a number of frame grabbers on the market. One decision you need to make here is whether to go with a digital camera which has a frame grabber on-board or to go to an analog camera with the frame grabber mounted in your computer. I'd suggest that you talk to a local system integrator (if you send me specifics on your location, I may be able to recommend a contact or two).
Two quick suggestions: Media Cybernetics has a complete system available (from camera to board to cables to software ... sort of "imaging in a box "). Just remember, you still need the Zeiss coupler or something similar (Diagnostic Instruments has great C-mounts with zoom focus).
Details are available the websites of these respective companies. Call/email me if you have any trouble finding them.
CAVEAT: MME has no commercial interest in any of these companies.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 10:12 AM 4/27/99 -0500, Chris Bradley wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
The New England Society for Microscopy will hold its Sixteenth Annual Spring Symposium at Marine Biological Laboratories, Woods Hole, Massachusetts on May 7 & 8,1999. In addition to our scientific presentations, this year's Symposium will feature PROEJCT MICRO, the new Great Explorations in Math and Science Program designed to introduce Microscopy to elementary- and middle school-aged students. If you are interested in participating in this exciting new program, be sure to register and join us for dinner on Friday evening. =
PRIZES for Best Poster, Best Student Poster, and Best Photo-As-Art will also be awarded. If you're interested in submitting your poster for judging, please contact Dr. Changmo Sung at Changmo_Sung-at-uml.edu.
A COMMERCIAL TABLE-TOP EXHIBIT is scheduled for Saturday morning from 10a= m to 12:30pm. =
The deadline for Exhibitor Registration has been extended to April 30th. =
Reserve your table now. The Spring Symposium always has a strong membership turnout and the Saturday Exhibition promises to be packed. =
Please double check to make sure you have registered so you do not miss this opportunity to visit old friends and make important new contacts. =
=
PROGRAM Friday, May 7th
12:00 pm Registration: Swope Center
1:00 pm Welcome: Lillie Auditorium
Session I Chairperson: Doug Taatjes, UVM
1:05 pm Laser Scanning Cytometry as an Imaging System Ed Luther, Senior Scientist - Biology, CompuCyte Corporation, Cambridge, MA
1:45 pm Focused Ion Beam Microscopy: The New Kid on the Block Dr. David Casey, Senior Scientist, Micrion Corp, Peabody, MA
2:25 pm Membrane Fusion Machinery in Cells Dr. Bhanu Jena, Dept. of Surgery and Biomedical Eng., Yale University, New Haven, CT
3:05 pm Afternoon Break Coffee served in Lillie 103
Session II Chairperson: Tony Garratt-Reed, M.I.T.
3:20 pm Multi-technique Characterization of Emissive Coatings on Electrod= es Dr. Chris Peters, Senior Project Engineer R & D, Osram Sylvania, Beverly, MA
4:00 pm Microscopic Approach to the Study of Pancreatic Islets Dr. Thomas Jetton, Ergo Scientific, Inc., Cambridge, MA
4:40 pm Advances in Microanalysis: A Look at the Past, Present and Future=
Dr. William Hardy, CEO, Princeton Gamma Tech, Princeton, NJ
5:30 pm Cocktails and Dinner: Swope Center
7:30 pm Project Micro "Festival" Meigs Room, Swope Center Dr. Burton E. Goodrich, Jr., Executive Director, South Coast Educational Collaborative Janet E. Goodrich, Principal, Miscoe Hill Elementary School, Mendon, MA
Saturday, May 8th
7 to 8:00 am Breakfast: Swope Center
Session III Chairperson: Eben Oldmixon, HSPH =
8:30 am The Use of Electron Microscopy in the Characterization of Carbon Deposits Paul Anderson, Chemistry Department, Northeastern University, Boston, MA
9:10 am Confocal Microscopy of Nuclear Envelope Breakdown Dr. Mark Terasaki, Asst. Prof., Dept. of Physiology, UCONN Health=
Ctr., Farmington, CT
10:00 am Commercial Exhibits and Posters: Swope Center Coffee and doughnuts will be served
12:30 pm Presentation of Poster and Photos-As-Art Awards and Door Prizes: =
Poster Area, Swope Center
1:00 pm Lunch with Short Tour of MBL: Swope Center =
2:00 pm 2-hour Discovery Cruise aboard the R/V Patriot II Tickets must be reserved in advance
Register Today! Contact L. Kirstein at NESM-at-compuserve.com. Registratio= n Deadline will be extended to April 30th. =
SILENT AUCTION, OCEANOGRAPHY CRUISE, POSTER AWARDS, DOOR PRIZES =
I have used this tissue processor for several years with good results, only draw back seem to do with resin polymerization in the vials if the protocol is for an extended period of time. -Mike
On Tue, 27 Apr 1999 msteglic-at-notes.mdacc.tmc.edu-at-Sparc5.Microscopy.Com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } I am currently looking at the EMS Lynx tissue processor and would like any } feedback from anyone currently using one } } (Do you like it?, Does it function as it should? Are there any drawbacks to } the unit? etc..). } } Does anyone know if there is a lab in the Houston area using one? } } Also does anyone know of any other tissue processor available for EM } processing? } } Mannie Steglich } U. T. M. D. Anderson Cancer Center } Houston, TX. } } } }
Hi guys, I need recommendation for 3D/deconvolution software, which will be able to run on NT. Thanks, --Ciprian ________________________________________________________________ Ciprian A. Almonte Phone: (412) 648-9796 Center for Biologic Imaging FAX: (412) 648-8330 University of Pittsburgh URL:http://sbic6.sbic.pitt.edu Pittsburgh, PA 15261 USA mailto:calmonte-at-pitt.edu ________________________________________________________________
We are consolidating our EM unit and must relutantly part ways with an exceptional H-600 Hitachi EM scope.
You may contact me directly through the e-mail address: rmonahan-at-caregroup.harvard.edu
or by phone at: 617-667-5777
Basic information on the instrument follows: Hitachi H-600 TEM The instrument is in excellent condition. It has been under service agreement with Hitachi.It has been used by a single experienced electron micriscopist to do strictly biological work.
Rita Monahan-Earley Senior Electron Microscopist Beth Israel Deaconess Medical Center Harvard Medical School 330 Brookline Ave Boston,Mass. 02215 USA E-mail:rmonahan-at-caregroup.harvard.edu Phone:617-667-5777
} Well, I hope we have helped, remember a motto "if a man built it a man can } fix it", no offence to the ladies as man is used in the generic sense. }
And keep in mind Mott's Law of Recursive Repair, which states that "In order to fix anything, you always have to fix something else first."
I have found this to be widely applicable, from software to old houses. Especially old houses...
} Dear All, } } We are in the market for a new sputter coater. We need a robust, } simple to use (i.e. student-proof) instrument for routine gold or Au/Pd } coating of biological samples. I would prefer to avoid micro-processor } controlled units (who's going to repair the circuit boards in 10 years?). } Does anyone have any advice, etc., etc.? } } Bob } } } Dr. Robert R. Wise } Department of Biology and Microbiology } University of Wisconsin-Oshkosh } Oshkosh, WI 54901 } } (920) 424-3404 tel } (920) 424-1101 fax } wise-at-uwosh.edu } www.uwosh.edu/departments/biology/wise/wise.html
Randy: I agree with Hank Adams on the OTOTO method, and have used Bob Kelley's method with great success for many years. The first critical part of the method is using a specific thiocarbohydrazide (not all suppliers/lots are equal), and doing the "wash" process for purifying the TCH just prior to use. The second critical step for success is being compulsive about the wash/time cycle in the published method. Any slighting of either of these steps can result in most unsatisfactory results. The conditions in the microscope are also critical, and I have obtained excellent results with the OTOTO method in both W filament SEMs at 10 to 30 kV as well as LaB6 and FESEMs at 1 to 2 kV. Hope this helps.
Roger Moretz Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT
The comments above reflect my own experience and opinions.
} -----Original Message----- } From: Tindall, Randy D. [SMTP:TindallR-at-missouri.edu] } Sent: Tuesday, April 27, 1999 12:26 PM } To: 'microscopy-at-sparc5.microscopy.com' } Subject: SEM: Fixation for non-conductive samples } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, } } I recall reading somewhere a long time ago about special fixation } procedures } for non-conductive samples that are hard to adequately coat. I seem to } remember tannic acid being involved. Does this ring a bell with anybody } out } there? } } Specifically, we are processing pig intestine samples for SEM and are } having } problems with charging on the microvilli. We have repeatedly coated the } samples with Au/Pd, but the problems persist. Perhaps there's a } specialized } processing technique that can help? } } Thanks in advance. } Randy } } } Randy Tindall } Electron Microscope Core } College of Veterinary Medicine } University of Missouri - Columbia } Phone: 573-882-8304 }
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Steve, We have a Gatan 791 MSC for our CM120. It takes lovely pictures. It did take me some time to master the art of acquiring the images. I took the Gatan class they offer in the spring, and it is definitely worth taking. Dr. Barbara Armbruster works in the California office and she took the time to help me work out my initial problems. She is a wonderful teacher. Let me know if you would like to see some images (pathology lab). Peggy Harger-Allen EM lab - VA Medical Center 317-554-0000x2392 harger-allen-at-indianapolis.va.gov
via smtpd (for sparc5.microscopy.com [206.69.208.10]) with SMTP; 28 Apr 1999 21:09:56 UT Received: from ptag2 (ptag2.pt.cyanamid.com [141.173.51.2]) by igate.cyanamid.com (8.9.3/8.9.3) with ESMTP id OAA02519 for {Microscopy-at-MSA.Microscopy.com} ; Wed, 28 Apr 1999 14:55:10 -0400 (EDT) Received: from ccmail.pt.Cyanamid.COM by pt.Cyanamid.COM (PMDF V5.1-9 #28913) id {01JAKJV6624W8X67N9-at-pt.Cyanamid.COM} for Microscopy-at-MSA.Microscopy.com; Wed, 28 Apr 1999 14:55:05 EDT
I need to examine some dissolution-recrystallization processes at temperatures in the 100-200 deg C range by transmitted light. I am looking for a way of making a cell, perhaps by gluing a metal washer with a hole of 3-4 mm onto a slide 26 x 26 mm to fit in a hot stage. I don't know if epoxy will withstand both heat and solvent. For another hotstage application, I once tried water glass for glass-to-glass, but it outgassed badly above 100 deg C.
I would appreciate any suggestions for other cements or from vendors with an all-glass cell, total height { 2 mm, cell ID 1-4 mm (round or rectangular), fittable on to or cut-downable to 26 x 26 mm.
Leonard Corwin Research Chemist Fort Dodge Animal Health Princeton, NJ 08543-0400
At 12:03 PM 4/27/99 GMT+1200, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi, I am trying to find a frame grabber that is able to grab images from a CCD camera and SEM(Amray 1830). The CCD camera is used for process monitoring through an optical microscope(Questar QM-100). A VCR is used to record the process on tape. After the process, sample is transfered into SEM chamber and I need to grab a few static images where particular features are found. Anyone has experience in this?
I need a copy of an old file called MSmouse.COM for an application that emulates this driver to reverse a mouse cursor on a DOS environment. The hard disk that had it crashed and the new computer does not have a 5 inch drive. Any idea of where I can get a copy will be appreciated.
*Disclaimer: Whatever... is not Tulane opinion! Cesar D. Fermin, Ph.D. Professor of Pathology & Otolaryngology web:[ http://www.tmc.tulane.edu/ferminlab/] or [http://www.tmc.tulane.edu/imaging/] Internet: [cfermin-at-mailhost.tcs.tulane.edu] 1430 Tulane Ave/SL79 New Orleans, La 70112-2699 Fax 504 587-7389, Voice 584-2521, Main Office 588-5224
There are many different systems on the market today, and they differ tremendously. You can purchase cameras that transfer the images serially, through a frame grabber, or by use of a different medium. Resolution and dynamic range of the cameras is an issue. What kind of software you need and the kind of support you expect.
For example: if you need high resolution images, you may have to consider a 3-chip TV camera or a digital color camera. For the latter you need to decide, if you can live with a low refresh rate or "offline" use (Images transferred to computer serially or by "sneaker net") or if you need a live image. If the cameras are too expensive, you may be able to acquire neighboring, overlapping images and do a multiple image montage with a less expensive camera, but that requires the appropriate software. Is it important to have automatically calibrated images? Do you need stage control, now or perhaps later? It is probably best to talk to other people who have image capture systems. Of course, as we sell those, we are happy to talk to you, too.
I would start, as Kalman Rubinson suggests, by defining the parameters for the system. Once you have done that, call a few vendors and discuss it with them. Depending on what they sell, they will suggest one or the other solution. That will help you narrow down the specs. I am sure, most vendors are happy to discuss this with you, I know, we would. Once you have narrowed down your choice, you can ask for quotes for similar systems and see, what the prices are.
If you need more help, give me a call or send me an email.
Michael Bode
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com
******************************************************* Disclaimer: Soft Imaging System produces and sells image acquisition and processing systems. We therefore have a vested interest in some of the items mentioned above. *******************************************************
} -----Original Message----- } From: Exchange Administrator } Sent: Wednesday, April 28, 1999 12:30 PM } To: Michael Bode } Subject: FW: CCD Camera and Image capture card } } } } ---------- } From: Kalman Rubinson[SMTP:kr4-at-is2.nyu.edu] } Sent: Tuesday, April 27, 1999 12:24 PM } To: Chris Bradley } Cc: Microscopy-at-sparc5.microscopy.com } Subject: Re: CCD Camera and Image capture card } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } On Tue, 27 Apr 1999, Chris Bradley wrote: } } } I am looking for a CCD camera and image capture card for use with a } standard Zeiss light } } microscope. I've been looking all over and I can't find any cameras } designed for this } } use. Can anyone help me out with the name of a vendor or a website? } } ???? Pretty much SOP today and all over the Web.*. Check } Edmunds or McCrone. Also, any C-mount camera can easily be } adapted to your microscope tube. The grabber cards are } ubiquitous as well. } } However, let me suggest you start the other way 'round. } Define your application needs and decide on the software } first. Then, choose grabber and camera from the supported } list. Otherwise, you may buy something which won't give } you what you need. } } Kal } }
Dear Microscopists I have a client who wants to use SEM to image the surface of corneas looking at scars after laser treatment. Any ideas on preparation, etc?? TIA Alan N Hall Laboratory for Microscopy and Micro-Analysis NWII Building University of Pretoria Pretoria 0002 Republic of South Africa Tel: +27-12-420 3896(Office) +27-12-420 2075(Laboratory) Fax: +27-12-362 5150
We are seeking to replace our stock of these, and e-mail enquiries to the company themselves have got nowhere. So (a) does anyone know a supplier, and (b) is there an alternative? We have used this type because it is the only organic material for filter membranes that can stand up to Xylene at 120^C, and the anodisc alumina filters in our catalogues are all too small in pore size.
Thanks in advance,
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
Can anyone tell me if there is a Canadian equivalent to the US. Project Micro and if so where I can find information or contact person? Thanks
Debbie Lietz Electron Microscopy Suite Department of Biology Trent University Peterborough, Ontario K9J 7B8 Telephone: (705)748-1486 Fax: (705) 748-1205 email: dlietz-at-trentu.ca
Have you tried a silver metal membrane? I believe these would be a suitable substitute. The company I am familiar with is Osmonics. A silver metal membrane with your required pore size can be found on their website at:
http://www.osmonics.com/products/Page2.htm
Hope this is helpful,
David Bell Scientist Electron Microscopy Lab Millipore Corporation 80 Ashby Road Bedford, MA 01730 (781) 533-2108
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Kal, Thanks for your comments. I did not originate this thread but have been looking for a general purpose digital camera for our multi-user facility for a few months. We have good high resolution video cameras and associated computer-enhancement capabilities and s-VHS recorders for live cell use so do not need video cameras. I find that fluorescence use is down as users turn to more confocal use. Thus our need for low light cameras is decreasing.
What I see is users who want to take a few images from a stereo microscope or light microscope and do not want to shoot a whole roll. So they do not document as much as they should. Addition of a relatively inexpensive digital camera may fill the need when the $10,000 for a more versatile camera + framegrabber+computer is not available. If funds are not a concern, the tendancy is often to go with the most versatile instruments one can find. However sometimes this leads to excess features, many of which are not needed and never used, and a waste of precious $$.
Also, recently there has been a call for cameras for student use in classes. For this purpose, we need multiple cameras but certainly do not want to designate a computer for each one and, again, cost is very important. Hopefully the two non-tethered camera I mentioned are just the beginnings of new offerings for this type of instrument.
I would appreciate hearing further comments from users (not venders) of these specific cameras and also would like to hear about other potential cameras to serve these needs (again from users rather than venders....I would appreciate the real on-the-job plus and minuses, not just the specifications).
Debby
--------------------------------------
} Olympus DP10 - uses a smart card to record images. Has a built in LCD } panel for formatting your image and a keypad for interacting with the } programming. You can print directly from the camera if desired. Resolution: } 1280x1024. Price ~$3000
If it records on SmartMedia, one does not need a frame grabber with it. I use a Lexar SmartMedia (parallel-port) reader. It functions like a removeable disc drive permitting one to read/write the SM. It cost me $37.
} Fugi HC-300Z: does not have built in LCD panel so it is helpful to attach } a small monitor for setting up your formatting, etc. Downloads images } directly to a ZIP disk. Has small keypad for interfacing with software. } Resolution: 1280 x 1000. Price ~$4000
Again. No grabber.
} Kodak also makes a microscope tube adapter for use with one of their } consumer cameras.
Also, it (DC-120) retains its non-removeable lens and has poorer resolution.
Consider also the Pixera and the MicroVision in this class of camera.
What all of these do NOT permit is the use of real-time image processing software. All processing is done post hoc. If this is OK in one's application, fine. Otherwise, one must consider a separate camera + grabber.
Kal
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RECEIVED: from SF_Database by POP_Mailbox_-1286883292 ; 27 APR 99 18:53:24 UT Received: from IS2.NYU.EDU by mailcenter.btny.purdue.edu with SMTP (QuickMail Pro Server for MacOS 1.1.2); 27-Apr-1999 18:53:20 -0500 Received: from localhost (kr4-at-localhost) by is2.nyu.edu (8.8.8/8.8.7) with SMTP id TAA09208; Tue, 27 Apr 1999 19:53:41 -0400 (EDT) Date: Tue, 27 Apr 1999 19:53:41 -0400 (EDT) From: Kalman Rubinson {kr4-at-is2.nyu.edu} To: Debby Sherman {sherman-at-btny.purdue.edu} cc: "message to: MSA list" {microscopy-at-sparc5.microscopy.com} Subject: RE: CCD camera and capture card In-Reply-To: {iss.2c27.37262849.ad7f2.1-at-scribe.cc.purdue.edu} Message-ID: {Pine.OSF.3.95.990427194804.7537A-100000-at-is2.nyu.edu} MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII
Are you looking to acquire images from a CCD camera AND an SEM, or is it one or the other?
} From your description ("a VCR is used to record...") I assume, that you are using an analog CCD camera. There are many frame grabbers on the market that will digitize these signals. Just look for "frame grabber" on the net, or give me a call.
If you wish to acquire images digitally from the SEM, the choice narrows down considerably, unless you only wish to use the SEM TV signal. In this case the same as for the TV camera applies. If you wish to acquire high resolution images from the SEM, you need some form of "active" or "passive" SEM interface. The reason is, that it is impossible to use TV standards (for example NTSC or PAL) for high resolution, slow scan images. So you need different hardware. As far as I know (perhaps I am wrong, in this case I apologize) we are the only ones that have "active" and "passive" acquisition boards that work as add-ons to our frame grabber. In other words, you can, with the appropriate software and hardware, use our frame grabber to acquire both TV signals AND control or synchronize to an SEM. Please give me a call if you neeed more information, or send me an email. If you want to check out our web site, look for "Grabbit" (our frame grabber) and "ADDA" (our SEM interface).
Michael Bode
******************************************************* Disclaimer: Soft Imaging System produces and sells image acquisition and processing systems. We therefore have a vested interest in some of the items mentioned above. *******************************************************
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: info-at-soft-imaging.com web: www.soft-imaging.com
} ---------- } From: Tong Wang[SMTP:tong-at-jlab.org] } Sent: Wednesday, April 28, 1999 4:39 PM } To: microscopy-at-sparc5.microscopy.com } Subject: frame grabber for SEM } } ---------------------------------------------------------------------- } -- } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -. } } } Hi, I am trying to find a frame grabber that is able to grab images } from a } CCD camera and SEM(Amray 1830). The CCD camera is used for process } monitoring through an optical microscope(Questar QM-100). } A VCR is used to record the process on tape. After the process, sample } is } transfered into SEM chamber and I need to grab a few static images } where } particular features are found. Anyone has experience in this? } } }
} I need a copy of an old file called MSmouse.COM for an application that } emulates this driver to reverse a mouse cursor on a DOS environment. The } hard disk that had it crashed and the new computer does not have a 5 inch } drive. Any idea of where I can get a copy will be appreciated.
Unfortunately, I deleted your earlier message but from Jim's response, it looks like your geologists need a combination of two techniques which are less than compatible. Not being very experienced with EM, I can't comment on the stages available, but in other applications (i.e., going from a fluorescence microscope to an FT-IR microscope), we have used finder stages which gave us the ability to exactly locate a specific feature then move it to the other microscope and find that feature again. If this were the case, your geologists could do their conventional polarized light analysis then move the system to the electron microscope for other imaging and analysis.
Hope this is helpful. Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** To find out what was new in microscopy at Pittcon, see "Focus on Microscopy", American Lab, May 1999
At 10:47 PM 4/28/99 +1000, Jim J Darley wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
The best way to keep an equal proportion of latex beads and virus particles is to centrifuge the mixing (well mixed) directly on a grid. For this, I use a Beckman Airfuge Ultracentrifuge with a A-100 rotor. The technique consist to mix a part of virus particles and a part of latex bead with a known concentration. Place in an Airfuge 200 µL-tube with a Formvar-carbon coated grid in the bottom. Centrifuge at 120 000 g (20 psi) during 5 min. Recuperate the grid, dry it, stain and finally count virus and beads in a TEM and compare the concentration of beads with concentration of virus particles see: - Alain, R et al., J. Virol. Meths, 16 (1987), p. 209-216 - Alain, R, Microscopy today, may, issue #97-4, D.Grimes Ed., (1997) p. 20
Robert Alain ********************************************************** Robert Alain, M.Sc. Microscopie électronique INRS-Institut Armand-Frappier-Microbiologie et Biotechnologie 531 boul. des Prairies Laval, Québec CANADA H7N 4Z3 Tel: (450)687-5010 ext#4388 Fax: (450)686-5626 e-mail: Robert.alain-at-INRS-iaf.uquebec.ca Http://www.iaf.uquebec.ca/iaf/recherche/viro/me.html **********************************************************
We are seeking vendors to provide some ocular micrometers (Olympus and Lietz eyepieces) as follows:
(1) grid configuration, divided into 400 squares (i.e., 20 x 20) (2) grid configuration, divided into 100 squares (10 x 10)
It is highly desirable to have the divisions numbered in the x-y directions.
Thanks.
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
Dear Bob, I recently acquired a Denton Desk II coater and etcher as part of a larger, used equiment purchase and I'm very pleased with it. It is much simpler to use than my very old Hummer was, very simple to operate and automatic enough for students. I just post a few lines of instructions on the wall behind it and everyone has had a good coat in 30 sec. so far. I like the simple target, which a flat gold sheet, which means that you don't have to get ripped off for a special target when it is consumed. I have been pleasantly surprised. I don't see any micro-processor control, just solenoid and relay. The system leaks the pump back to vacuum when you turn it off, so you don't suck oil vapours back into the chamber, something I could never teach students to do. You wrote: } } } Dear All, } } } } We are in the market for a new sputter coater. We need a robust, } } simple to use (i.e. student-proof) instrument for routine gold or Au/Pd } } coating of biological samples. I would prefer to avoid micro-processor } } controlled units (who's going to repair the circuit boards in 10 years?). } } Does anyone have any advice, etc., etc.? } } } } Bob } } } } } } Dr. Robert R. Wise } } Department of Biology and Microbiology } } University of Wisconsin-Oshkosh } } Oshkosh, WI 54901
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I am an experienced electronics design engineer who wants to take on building something a bit more challenging in his garage: A scanning Electron Microscope.
I already have information on building diffusion pumps ( although more information is always welcome ), and I have info. on construction and testing of high vacuum apparatus.
The electronics will be straightforward enough.
What I need is help on electron optics. Are there any "beginners guide to designing electron optics" or "A home made SEM: how I did it" books out there? Can anyone suggest how I get from undergraduate physics to SEM design ( I learn well from books )?
Dear John, We've been using eyepiece reticules from Klarman Rulings, POB 4795, Manchester, NH 03108, (800)252-2401. They are not expensive, available in any diameter with a wide range of styles, and accurate.
Glen
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
On Thu, 29 Apr 1999, John J. Bozzola wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We are seeking vendors to provide some ocular micrometers (Olympus and } Lietz eyepieces) as follows: } } (1) grid configuration, divided into 400 squares (i.e., 20 x 20) } (2) grid configuration, divided into 100 squares (10 x 10) } } It is highly desirable to have the divisions numbered in the x-y directions. } } Thanks. } } } #################################################################### } John J. Bozzola, Ph.D., Director } Center for Electron Microscopy } 750 Communications Drive - MC 4402 } Southern Illinois University } Carbondale, IL 62901 U.S.A. } Phone: 618-453-3730 } Fax: 618-453-2665 } Email: bozzola-at-siu.edu } Web: http://www.siu.edu/departments/shops/cem.html } #################################################################### } } } }
Thanks, Greg, I'll consider these lines of thinking. We have tried going from acetone into HM-20 (I think that's what was used; it was over a year ago), not sure how it would work directly, have to look into that. I'm pessimistic about the membrane connections holding up after freeze-drying and then re-wetting with resin. Will see if I can read up on what's been done.
We have done freeze-fracture-etch but the details I want to see (connections between membranes) do not show up for various reasons with that technique. I guess something to look into is the possibility of cryo TEM with energy filtering and 3-d reconstruction, but my specimens are so thick (~15 micron diameter) that, as I understand it, it's too thick for the usual technique so maybe I'd have to see if this could be done with HVEM. Thanks again for your suggestions Richard p.s. Fats and lipids are a nightmare for lots of things, but that's my job. I vividly remember a biochem professor trashing one of my class proposals because they're hard to work with. That's what makes it interesting!
} } } Greg Erdos {gwe-at-biotech.ufl.edu} 04/27/99 12:20pm } } } Regarding TEM of fat, I might suggest two possible approaches. One would be to use a hydrophobic resin like Lowicryl HM-20 or HM-11. It might even serve as the substitution media. Another thought is to freeze dry the samples and embedd them in resin directly . One could also expose the sample, after drying, to osmium vapors from the crystals in order to keep things anhydrous. I would recommend a resin that can tolerate a little water, like Epon or its equivalent, or a methacrylate based resin.
Would using freeze-fracture EM give the results you are after??
Fats and lipids are a nightmare for EM.
Greg Erdos Gregory W. Erdos, Ph.D. Ph. 352-392-1295 Assistant Director, Biotechnology Program PO Box 110580 Fax: 352-846-0251 University of Florida Gainesville, FL 32611
I have just been asked to submit budget numbers for a replacement SEM. We have a DS-130 with tungsten filament. I have not been keeping up on the latest and greatest and I would like to hear from end users who have recently purchase a SEM. Our needs are almost all related to inorganic and metallurgical samples. Thanks for your time.
Bruce Kaye
Dockyard Laboratory Pacific (DL(P)) Building 199 Dockyard PO Box 17000 Stn Forces Victoria BC V9A 7N2 CANADA (250) 363-2514 Fax: (250) 363-2856 Email: bruce.kaye-at-edrd.dnd.ca
I did a TEM in high school....many years ago. It was based on an RCA EMU. The cost today of a fine SEM platform is about $5K. With that, you get console, column, turbo, roughing pump, etc., etc. I would not imagine that any current efforts at trying to achieve what has already been achieve would be worth more than self gratification. If that is your goal, go for it. If you really want to see what a SEM can do and want to improve on it, get a good column and start working on digital controls and capture. That avoids reinventing the wheel and truly is where the future is as I see it.
} } } The last few times I"ve made up a section-staining solution of uranyl } acetate, (J.E.M.Techniques 16,81(1990)), an abundant brown precipitate } forms within the syringe in a day or two. It usually stays fine for at least a month or even more. } I've tried both a glass syringe and a polypro syringe with similar }
I want to buy an used SEM. I'm looking now on a Hitachi S-530 model. Is there someone that can give me their experience with that SEM model ? What I should look to make the best deal ? Thank you.
I want to buy a DIAMS to grabbe image from a SEM, from a optical microscope, and from a stereoscope. I'm evaluating now the Quartz PCI system from Quartz Imaging Corporation, and the Clemex Acquisition and R'Kive System from Clemex Company. Is there someone that can give me their experience with those DIAMS model ? Thank you.
I remember that someone asked about slide markers recently. If you look on EBAY the internet auction site there is one for sale. It is a Leitz diamond marker and the item# is 95912618. The price so far is $50 but it has an unknown reserve.
It is difficult to add something to Steve has told. But I have pricked my ears after your words that the diffusion pump is not too hot. The point is that diffusion pump works only, when its bottom is too hot, and top is too cold. If the diffusion pump does not pump, any HV turning on can led to glow discharge and to overloading of HV supply unit. Pirani gauge is too rough meter and requires often adjustment to estimate vacuum.
Regards. Victor Sidorenko, ANTRON, Moscow, Russia.
Renata Korzyniewski wrote:
} Dear Listservers, } } We have a Hitachi S-520 SEM installed in 1983. Lately, we have experienced } instability problems in the SEM and I'm not sure what to do. The Hitachi } S-520 SEM has a single rotary pump, single diffusion pump and Pirani } gauge. On initial pumping and warm-up, the SEM gets down to high vacuum } (reading 10uA) quite normally in about 20min. On applying HT (20kV), } everything appears normal for approximately 80min, then, suddenly the HT } switches itself off and the vacuum gauge rises to 15uA in 30sec and then } falls back to10uA in 2min. If the slightest gun emission current is } applied when the SEM says it's ready, the HT instantly switches itself } off. I have cleaned the filament assembly, anode and gun chamber around } the anode. However, I have not cleaned the insulator at the top of } the gun chamber. The Pirani gauge is clean and the diffusion pump is } not too hot. Any advice would be much appreciated and gratefully } received. Thankyou. } } John Brealey Queen Elizabeth Hospital EM Unit Adelaide South Australia }
EuroFE '99 is a forum to bring together interested parties in the field of Field Emission Technologies. There is also a strong emphasis on links with other display technologies such as LCD's, Light Emitting Polymers and Phosphors. The aims of this Workshop are to:
Present the activity of each group in Europe working in Field Emission (FE) research and related areas. Present the EuroFE network to the Scientific Community and Brussels and enhance its activity. Initiate, through this meeting, groups with several partners in order to present projects to Brussels. Enhance the participation of companies in the EuroFE Network and EU programs. Exchange knowledge & initiate collaboration between groups either Institutes or Companies (manufacturing joint ventures, collaborative research, development activities, etc.). Allow important student participation.
The scientific programme will cover the whole spectrum of Field Emission research and related areas including keynote lectures, oral presentations, posters and a product/instrument exhibition. Topics of interest will be for example:
Field Emission device characterisation (surface analysis, etc.) Industrial applications (Flat panel displays, etc.) Field Emission from diamond, DLC and nanotubes FE simulation and modelling FE microwave applications Novel cathodes - technology and fundamentals Other related areas (phosphors, new materials, novel devices, LCD, etc.)
For further information please see http://www.cmp-cientifica.com/EuroFE/eurofe99.htm Or contact me direct.
Renata Korzyniewski wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Listservers, } } We have a Hitachi S-520 SEM installed in 1983. Lately, we have experienced } instability problems in the SEM and I'm not sure what to do. The Hitachi } S-520 SEM has a single rotary pump, single diffusion pump and Pirani } gauge. On initial pumping and warm-up, the SEM gets down to high vacuum } (reading 10uA) quite normally in about 20min. On applying HT (20kV), } everything appears normal for approximately 80min, then, suddenly the HT } switches itself off and the vacuum gauge rises to 15uA in 30sec and then } falls back to10uA in 2min. If the slightest gun emission current is } applied when the SEM says it's ready, the HT instantly switches itself } off. I have cleaned the filament assembly, anode and gun chamber around } the anode. However, I have not cleaned the insulator at the top of } the gun chamber. The Pirani gauge is clean and the diffusion pump is } not too hot. Any advice would be much appreciated and gratefully } received. Thankyou. } } John Brealey Queen Elizabeth Hospital EM Unit Adelaide South Australia
Hi Renata or John, Do you know if your diffusion pump has a full charge of oil? I've seen this type of vacuum behavior when the charge gets low. Things start out pumping fine, but then the system gets all of the oil up in the pump and on the side-walls. It stops pumping until some of that oil makes it back to the sump where it is re-boiled. A short burst of pumping occurs.
Might be an easier thing to check than tearing apart potted HV components for starters.
Ken Converse owner Quality Images third party SEM service Delta, PA
EMBO Practical Course on Biophysical and Mathematical Approaches to Cell Biology
4 July - 17 July 1999 -at- EMBL, Heidelberg, Germany
ORGANIZERS: Ernst H.K. Stelzer, Michael Way, J.K. Heinrich H=F6rber
The links between physics and cell biology are becoming more and more important. In many instances it is no longer sufficient to test a model quantitatively. Many processes such as microtubule dynamics (i.e. the microtubule assembly and disassembly) have only been understood since a) = the in vitro system was sufficiently well developed, b) a mathematical model that describes the events became available, and c) the events could be recorded, analyzed and compared with the model. This course is aimed fir= st of all at researchers working in the life sciences who wish to improve th= eir mathematical background and move into a more direct use of physics for quantitative biology. They wish to acquire a mathematical/physical framework for their biological system of interest and need to know how to verify it in a quantitative manner. However, another equally important group of attendees has a background in mathematics and/or physics and wis= hes to understand the problems of working with biological systems in a quantitative manner. One of the main objectives of this EMBO course is t= o improve the communication between biologists and physicists.
Speakers from the Americas and Europe including the staff at EMBL (Boulin= , Dotti, Eaton, Florin, Gonz=E1lez, Griffiths, H=F6rber, Hyman, Karsenti, N= ilsson, Pepperkok, Simons, Stelzer, Vernos, Way, Zerial) will introduce students = to: microtubule dynamics, determining growth shrinkage/rates, generating microtubule patterns, molecular motors, mechanisms of neuronal plasma membrane asymmetry, microtubule organization during development, complex membrane organelles, chromosome movement at mitosis, mitotic spindle assembly, distributions of proteins along exocytic pathway, surface polar= ity in epithelial cells, actin cytoskeleton and cell motility, membrane traff= ic in eukaryotic cells, cell polarity in Drosophila, single molecule force measurements, molecular mechanics. Further topics may be added.
The deadline for applications is 15 May 1999. The organizers will select the students. Their decision is final. Industry applications will be considered. The course participants will come from all over Europe. The selected students will be informed by 21 May 1999. The main selection criteria are: degree in natural sciences, exposure to modern cell biology for at least one year, research is related to progress in biology, and th= e ability to convince the organizers of an interest in this course by CV an= d covering letter. Students will not be exclusively post-doctoral.
Applications should be sent in printed or electronic form to: Dr. Ernst H= .K. Stelzer, European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Programme, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. stelzer-at-embl-heidelberg.de, fax +49 (6221) 387306. http://www.embl-heidelberg.de/ExternalInfo/stelzer/Courses/CellBiologyBio= phy sics
Sincerely Yours E. Stelzer
Dr. Ernst H.K. Stelzer EMBL-Heidelberg Light Microscopy Group Cell Biophysics Programme Meyerhofstrasse 1 D-69117 Heidelberg Postfach 10.2209 D-69012 Heidelberg Germany Phone +49-6221-387 354 Fax +49-6221-387 306
I'd like to find a used SEM. Since I currently have an ISI, an old SX-30 or 40 would be perfect, as I have quite a bit of ISI apparatus. I'm certainly willing to switch manufacturers if I can find a good small to medium size scope.
If any of you have a SEM in your garage that you hoped to bring back online someday, but are being nagged to get rid of it, this is your chance to make a big dent in the spring cleaning! Please make a proposal to jbest-at-elmdas.com, and not the list server. Thank You.
Jianli, Try cooling the sample slowly in liquid N2 and then lightly scribing the surface under the liquid N2 with a diamond scribe. This may flake off the film in small areas providing an edge for scanning. Make sure the adjacent film is not delaminated as this would give a false reading. Also the surface should be cleaned to remove lose particles e.g. CO2 snow cleaning works great for this. Good luck. Russ Xerox
-----Original Message----- } From: Jianli Wang [mailto:jiwang4-at-mail.vt.edu] Sent: Thursday, April 29, 1999 5:02 PM To: spm-digest-anal-at-bbfm.di.com
************************************************************** THE UNIVERSITY OF LEEDS
DEPARTMENT OF MATERIALS, SCHOOL OF PROCESS, ENVIRONMENTAL AND MATERIALS ENGINEERING.
RESEARCH OPPORTUNITY IN HIGH SPATIAL RESOLUTION MATERIALS CHARACTERISATION
RESEARCH FELLOW
The above HEFCE-funded post is available from October 1 1999 for a fixed period of three years to commission, support and apply an optimized, analytical field emission transmission electron microscope for materials analysis to a wide range of materials projects based at Leeds and in regional institutions.
Applicants should have a PhD in physical/engineering sciences and research experience in Materials, Physics or Chemistry involving particularly Transmission Electron Microscopy and Microanalysis.
Salary: Research Staff Grade 1A (stlg15,735 - stlg23,651 p.a.) according to qualifications and relevant experience.
Application forms and further particulars may be obtained from:
Ms Joy Bielby, Department of Materials, School of Process, Environmental and Materials Engineering, University of Leeds, Leeds, LS2 9JT, United Kingdom.
In all enquiries please quote the reference number 062-069-004-027
Informal enquiries on the post should be directed to Dr R. Brydson (email: mtlrmdb-at-leeds.ac.uk)
Closing date for applications 24 July 1999
Towards Equal Opportunities. **************************************************************
_____________________________ Dr. Rik Brydson, University Research Fellow, Electron Optical Unit, Department of Materials, School of Process, Environmental and Materials Engineering University of Leeds, Leeds LS2 9JT, U.K.
We recently acquired a digital camera from Advanced Microscopy Techniques (AMT) and are extremely pleased with it. AMTs model 12-HR uses a Hammamatsu camera, custom lens and AMTs own software. The camera has an electronic shutter and manages better than 10 frames per sec at lower illumination than most users would use by eye on the fluorescent screen. Sharpness is excellent, and indeed publication quality. Support and vendor interaction are simply outstanding - it is hard to imagine it being any better. This is in stark contrast to the treatment our institution has received from Gatan - who says that since they pitch in a few hundred dollars to support our local microscopy group that it should be sufficient support for the local users!
Software: Admittedly, Gatans software is a mature package, whereas the AMT software is undergoing rapid revisions. However, the AMT software works perfectly and meets all of our needs - as biologists in a busy core facility. If we had chosen Gatan, each of our users would need to spend nearly $600 apiece just to view the extra image data. With AMT they can see their image as well as all recorded data (voltage, mag, comments, etc) with any tiff file reader.
There are several other reasons why I would buy AMT over Gatan any day, but it would be best if you contacted me off-line. I am sure just what I have said here is going to ruffle a few Gatan feathers.
Regards, Jim
-------------------------------------------------- James F. Sanzo, Ph.D. Codirector, Biomedical Imaging Core Laboratory B-110 Richards Building University of Pennsylvania 36th and Hamilton Walk Philadelphia, PA 19104-6085 Voice: (215) 898-6730 Fax: (215) 573-2259
You don't say where you are located, but a few hours on the phone should be able to locate a free or cheap microscope sitting on somebody's loading dock. Repairing somebody else's design might not be what you are interested in, but in this case you at least don't need to build your own pump, chamber, high voltage power supply, etc.
But, if you want to go the other way and see what can be done from scratch that is fun too. Most of the literature on the technology you are trying to duplicate is pre-1970, so the on-line literature searches won't be much help. There is a lot of practical knowledge of electron optics in the Review of Scientific Instruments and the old Journal of Scientific Instruments. The old electron optics books such as Klemperer can be useful if you don't get bogged down in the details.
As for a gun, cutting off the neck of a crt, and using the video scanning coils would be a good place to start.
Or maybe better, get a vidicon based tv camera and take off the window.
best regards mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
} Hi guys, } I need recommendation for 3D/deconvolution software, which will be able to run on NT.
Ciprian A. Almonte Center for Biologic Imaging
Mr. Almonte,
The appropriate product we would suggest you use would be VayTek. Our suggestion is for you to check our web site www.i-cubeinc.com Then we can provide you with a specific recommendation.
Regards
Barry Dudley
-- ************************************************************ B.T. DUDLEY I-CUBE www.i-cubeinc.com Ph 1-888-77-I-CUBE 301-858-0505 301-858-0615 (Fax) I-CUBE is a Systems Integrator and Value Added Reseller of image analysis and image processing products for scientific and industrial applications. We provide a single source for imaging products, using a consultative selling approach I-CUBE 2411 Crofton Lane; Suite 14A; Crofton; Maryland; 21114 ************************************************************
The Minnesota Microscopy Society will hold its annual Spring Symposium on May 13th in the Sheraton Midway Hotel (midway between St. Paul and Minneapolis). Full details of the meeting can be found on our website http://resolution.umn,edu/MMS/ but the highlights are as follows:
MMS Spring Symposium: Latest Trends in Microscopy Thursday, May 13, 1999
Location: Sheraton Midway Hotel, located at the intersection of I94 and Hamline Avenue.
Program
8:00 - 9:00 Registration with refreshments 9:00 - 9:45 Microcalorimeter EDS with 3 eV Energy Resolution David A. Wollman, NIST 9:45 - 10:30 Innovations in Energy-Dispersive X-ray Microanalysis John J. Friel, PGT 10:30 - 11:15 Break and Vendor Displays 11:15 - 12:00 Recent Advances in Microwave Assisted Specimen Processing: Rapid Preparation of Biological Specimens for Correlative Confocal and Electron Microscopy. Mark A. Sanders, U of M 12:00 - 1:00 Lunch Provided 1:00 - 1:30 MMS Business Meeting 1:30 - 2:15 Application of SEM Diffraction to Phase Identification Pat Camus, NORAN 2:15 - 3:00 Break and Vendor Displays 3:00 - 3:45 Imaging Mechanisms in Dynamic Force Microscopy of Polymers Greg D. Haugstad, U of M
Reservation/ Cost: Registration is $50 for members, $60 for non-member (this includes a one year membership fee). To make reservations, please contact Mike Coscio at 612-514-1331, or by e-mail at mike.coscio-at-medtronic.com.
__________________ Stuart McKernan stuartm-at-tc.umn.edu Microscopy Specialist Office:(612) 626-7594 CIE Characterization Facility, University of Minnesota Desk: (612) 624-6009 100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590
I would be interested in hearing about the value of service contracts. Our SEM (Hitachi S-3100H) is about 2.5 years old and has been operating without problems. If I operated without a service contract, handling all preventitive maintenance myself, am I begging for trouble? Those of you who don't operate with a service contract have you found the cost of emergency services so expensive that a service contract would have been preferable? Do you find the service difficult to get without a contract? Are the parts that would need replacing terribly expensive (more than the cost of the service contract)? As the instrument gets older is there a greater concern? Is my concern about the cost of the service contract unwarranted?
Thanks.
David Rose W.L. Gore & Associates 297 Blue Ball Road Elkton, MD 21921
Dear Marc, I have used the Quartz PCI system for some years and it should be able to do everything you want to do. I only have two SEM's and a TWAIN flat-bed scanner on mine, but the icons and tools for video cameras, digital cameras and other devices are all there and active all the time. I know others who use SEM and video acquisition iinto the same system and it works perfectly. The database allows you to organize all the images and search for them by job, session, job or any search string you like. I have over 2800 images in my database from two SEM's and the scanner. You wrote:
} } I want to buy a DIAMS to grabbe image from a SEM, from a optical } microscope, and from a stereoscope. I'm evaluating now the Quartz PCI } system from Quartz Imaging Corporation, and the Clemex Acquisition and } R'Kive System from Clemex Company. Is there someone that can give me } their experience with those DIAMS model ? Thank you. } } Marc Montreuil } Lachine (Qu=E9bec) Canada } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
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Your idea can be considered as a part of mine one about global knowledge base as self-growing unified searching source of information. It is described at my homepage, link in signature. It is in baby state still, and I would be glad to receive comments from anyone in the list on how to move it further.
Dmitri.
} Hi all, some toughts: } } a database containing user manuals for a range of older popular microscopes } would be a very good idea, especially since the purchase of a second hand } microscope is usualy the only way for an amateur microscopist to get hold of } a decent microscope at a (more or less) reasonable price. That's why I have } put some Reichert Zetopan manuals on my website. } } In the best case scenario this service should be easy accesible and free if } at all possible. If that isn't possible a small fee could be asked to cover } the costs. } } But it isn't simple: } } I don't think that the large manufacturers would like to support this idea, } as it isn't in their short-term intrests to support users of second-hand } equipment, they rather like to sell new gear (one example: I know from a } very reliable German source, that Zeiss has, after the "wende", destroyed } large stocks of spare parts for older "Eastern-German Zeiss } manufactureres"). I think they see it the wrong way: I can't imagine much } amateurs who spend the exorbitant prices the large manufactureres ask for } their products, at least I wouldn't... } } And, unfortunally, as far as I know, the manufacturers are the owners of the } copyrights of their manuals, so we're stuck here, that would be the first } problem to be solved... } } After that: finding someone to coordinate the project, finding the manuals } and scan those (can't be much of a problem I suppose), finding a server to } host the documents and finding some people to do investigations regarding } brands, models, serial numbers... to match manuals and models/versions... } } I would like to volunteer for such a project... } } Hello Royal microscopical society (England), Microscopy Society of America, } German microscopy clubs, Micscape Magazine, manufacturerers...? } } Yvan Lindekens.
__________________________________________ Dmitri V. Sokolov, Doctor Course student Research Center for Interface Quantum Electronics, Hokkaido University, North 13, West 8, Kitaku, Sapporo 060, Hokkaido, Japan Phone 81-11-706-7174 Fax 81-11-716-6004 http://www.geocities.com/SiliconValley/Campus/1314 AOL Instant Messenger FalconDot ICQ 9418072 __________________________________________
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