A colleague wants to prepare coral eggs for TEM, with particular interest in the plasma membrane. They have been fixed in 3.3% Formalin for 15 minutes, then stored at 4 degrees in seawater ( for about 3 days ).
Has anyone out there prepared these?
Any tips, tricks, and pointers to appropriate literature will be greatly appreciated.
With thanks,
Rosey van Driel
Rosemary van Driel Electron Microscopist
Baker Medical Research Institute PO Box 6492 St. Kilda Rd. Central Melbourne Victoria 8008 Australia
Dear all, Not directly related to microscopy, more to how to write up the results.
Does anyone know how to set up a field in Microsoft Word 98 for Mac to get something like a bar-1 or bar-2 for Miller indices?
I know this was discussed in '94 but those fixes don't quite seem to work the same on the latest version of Word. I tried the help function and it was as helpful as usual (i.e. lots of information but not much real help).
I hope someone out there can help. Using equation editor every time can get pretty tedious.
Thanks in advance
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren Department of Experimental Physics Chalmers University of Technology S-412 96 G=F6teborg Sweden Tel: +46 31 772 36 33 FAX: +46 31 772 32 24 email: maclaren-at-fy.chalmers.se or: ian.maclaren-at-physics.org Research Group Homepage: http://fy.chalmers.se/microscopy/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
I attach details of a 3 year post doc. position in the=20 Department of Materials at Oxford (UK). This project will=20 be based around our JEOL 2010 and JEOL 3000F TEMs. If you=20 need further information please contact Beverley directly.
Ron ___________________________________________________________
DEPARTMENT OF MATERIALS, UNIVERSITY OF OXFORD Post-doctoral Research Position Grade RS1a:Salary =A316,288 - =A324,479
*********************************************************** Nanoscale deformation of materials quantified by TEM=20 nanoindentation
A three-year post-doctoral research assistantship funded by=20 the EPSRC is available in the Department of Materials at=20 Oxford from January 2000 (or as soon as possible=20 thereafter). The EPSRC project involves the design,=20 construction and operation of a novel nanoindenter to fit=20 into a high resolution electron microscope. The TEM=20 nanoindenter will be used to impact materials in-situ=20 whilst observing in real-time and with nanometre=20 resolution. This is an exciting new technique to quantify=20 the fundamental mechanisms of deformation, friction and=20 wear at the nanoscale. The person appointed will have=20 research experience of AFM and/or nanoindentation=20 instrumentation, and familiarity with TEM and=20 nanostructured materials would be desirable.
Further particulars are available from the Administrator,=20 Department of Materials, Parks Road, Oxford, OX1 3PH, UK,=20 to whom applications including a CV, list of publications,=20 and the names and addresses of three referees should be=20 sent, quoting Ref. BJI. Further particulars are also=20 available by email: beverley.inkson-at-materials.ox.ac.uk. The=20 closing date for applications is 6th December.=20
Tousimis Research Corporation sells a filter housing and replacement cartridge that sounds like the unit that you describe. I have been using one for years.
Tousimis PO Box 2189 Rockville, MD -2189 1-800-638-9558 www.tousimis.com
Louie Kerr
At 3:24 PM -0700 10/28/99, William P. Sharp wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Louie Kerr Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
Could anyone offer a used double-tilt holder in good condition for JEOL 2000FX? If yes, at what price? It would be used at University "Eotvos", Budapest, Hungary
Thanks in advance:
Dr. Janos L. Labar Research Institute for Technical Physics and Materials Science H-1121 Budapest, Konkoly-Thege u. 29-33 Postal address: H-1525 Budapest-114, Po Box 49 Tel: (36)(1) 395-92-20 / 24-21 ext. Fax/phone: (36)(1) 395-92-32 home page: www.mfa.kfki.hu/~labar
Ian, This is quite easily done (although step 1 may be difficult). 1. buy a PC. 2. in Word, got to Insert Symbol 3. go to Font: Lucida Sans Unicode 4. go to Subset: Combining Diacritical Marks 5. choose the 6th diacritical mark -- i.e. the one at the end of row 21 of the symbol table. 6. insert (after the character you wish to bar). 7. for convenience, set a shortcut key after step 5. I use Alt+Shift+Q (only because it is close to the Alt+Shift+A that I use for Angstrom). But the first step is a big one! -Mike
"Ian MacLaren" {maclaren-at-fy.chalmers.se} wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Ian, This is quite easily done (although step 1 may be difficult). 1. buy a PC. 2. in Word, go to Insert Symbol 3. go to Font: Lucida Sans Unicode 4. go to Subset: Combining Diacritical Marks 5. choose the 6th diacritical mark -- i.e. the one at the end of row 21 of the symbol table. 6. insert (after the character you wish to bar). 7. for convenience, set a shortcut key after step 5. I use Alt+Shift+Q (only because it is close to the Alt+Shift+A that I use for Angstrom). But the first step is a big one! -Mike
"Ian MacLaren" {maclaren-at-fy.chalmers.se} wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I have been given a Beck microscope Model 4196 which has a set of three reflecting (mirror) objectives. Instead of the normal tube between the objective and eyepiece the microscope has a bellows allowing tube length to be varied over a wide range.
I have no documentation on this microscope, and would be most interested to know whether anyone knows of it, and can tell me what it was used for.
Chris Jeffree ===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
Dear Fellow Microscopists, First of all allow me to thank you all for all your responses for my query on sputter coaters and vacuum evaporators. One of the models we are currently considering is the Denton Desk II TSC cold sputter/etch unit. Is anyone out there that operates one? If so, what is your opinion of the unit in terms of pump-down time, quality of both sputter and carbon coat, reliability and any other feature that you like or dislike?
Thank you, Maria
Maria Fazio-Zanakis Bioimaging and Molecular Histology Hoechst Marion Roussel, Inc. 1-908-231-3357 Fax: 1-908-231-3962 Email: Maria.Fazio-Zanakis-at-hmrag.com
Below are brief notes on virus ID (comments on human viruses, but may be useful for other critters as well. Refs below. Name and phone # below if you have further ques. Good luck.
Brief Summary Dx Virology by EM
Methods: 2 ways to look -at- viruses: 1. Negative stains of Liquids (size, surface structure important) 2. Thin sections of Tissues (internal structure and cell organelle-association important) Thus, 2 prep methods and 2 morphological pictures to consider
Genetically 2 kinds of viruses: DNA/RNA Morphologically 2 kinds of viruses: naked/enveloped
Enveloped (have pliable membrane around outside, usually derived by budding through cellular membranes) Size: 40-300 nm
Spiked (have spikes or fuzz on outside) (e.g. influenza virus)
Smooth (spikes are too short to see by routine EM) (e.g. herpesviruses, rubella virus)
Nucleocapsid inside (nucleic acid plus some proteins to hold it together) Icosahedral Helical Complex Morphologically nondescript
Association of virus with different cellular membranes may be a clue to ID Plasma membrane ER Vacuoles Nulcear membrane
Most are pleomorphic, but 3 have a distinctive shape: poxvirus (brick-shaped), rhabdovirus (bullet-shaped), filovirus (like rhabdo but very long-/1400 nm)
Location within cell clue to nucieic acid type Nucleus: DNA viruses Cytoplasm: RNA viruses
Some exceptions to RDNA virus in nucleus/RNA virus in cytoplasm:S Naked viruses get out of the cell by lysis; if the cell is very sick, there may be virions everywhere, e.g. adenovirus (DNA).
Pox viruses are DNA viruses, but are always constructed in the cytoplasm.
Hepatitis B core particles (DNA) may be in the nucleus and cytoplasm.
Some paramyxovirus (RNA) nucleocapsids, not the whole virus, can be seen in the nucleus, e.g. measlesvirus.
References on Virus identification by electron microscopy
1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses associated with HIV infection in Tanzanian children with chronic diarrhea. Ped AIDS HIV Inf 5:296-299.
2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic Virology: A Practical Guide and Atlas, Cambridge University Press, New York.
3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE, Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro LS, and Jaqua M-J. 1990. Six year retrospective surveillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada Pediatr Infect Dis J 9:709-714.
4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus Infections, CRC Press, Boca Raton, FL.
5. Miller SE. 1986. Detection and identification of viruses by electron microscopy. J Electron Microsc Tech 4:265-301.
5. Miller SE, Howell DN. 1988. Viral infections in the acquired immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.
7. Miller SE. 1988. Diagnostic virology by electron microscopy. ASM News 54:475-481.
8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis. EMSA Bull 19:53-59.
9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and Methods. McGraw-Hill, New York.
10. Miller SE. 1991. Evaluation of electron microscopic information available from clinical samples. In de la Maza LM, Peterson EM (eds.), Medical Virology, Vol 10. Irvine, CA. pp 21-54.
11. Miller SE. 1995. Diagnosis of viral infection by electron microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, American Public Health Assoc, Washington, DC. pp 35-76.
12. Oshiro LS, Miller SE. 1992. Application of electron microscopy to the diagnosis of viral infections. In Lennette EH (ed), Laboratory Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.
13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC Press, Boca Raton, FL.
14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral Diagnosis, CRC Press, Boca Raton, FL.
15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, New York.
2, 5, 9, 11-14 = Methods and micrographs 2, 14 = Excellent atlases 7, 8 = Brief synopses of technique and basis of virus ID 15 = Good general reference, but don't memorize!! Lists all viruses, not just human.
Also check Hans Ackermann for bacteriophages. Not sure about protozoans, but run Medline or PubMed search crossing protozoans and viruses.
On Fri, 29 Oct 1999, William McManus wrote:
} Date: Fri, 29 Oct 1999 13:07:57 -0600 } From: William McManus {billemac-at-biology.usu.edu} } To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com} } Subject: identifying virus in tissue? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } To all listers: } } We have a very limited amount of protozoan sample which appear to have } virus-like particles within the cells, but we need to know more conclusively } if the particles are virus before we continue. Does anyone know of a method } using EM to identify virus in tissue? We presently have material available } and could run either a pre or post embedding technique. } } William McManus } Supervisor } Electron Microscopy Facility } Department of Biology } Utah State University } Logan UT 84322-5305 } } billEMac-at-cc.usu.edu } 435-797-1920 } } }
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
It's actually pretty simple. Try the following (I did this in Office 9= 7)
1. create your bar-1 (or even a more advanced equation) with the equat= ion editor 2. select the equation by clicking it once 3. go to Tools/Autocorrect in Word (The "with" box should already hav= e the equation in it) 4. type something into the "replace" box--for example "bar1" 5. click OK
Now, just type bar1 and when you hit the space bar afterward, the equat= ion is inserted.
hth, Jim Passmore Cryovac Division Sealed Air Corporation
"Ian MacLaren" {maclaren-at-fy.chalmers.se} on 11-01-99 05:20:41 AM = =20 = =20 = =20
Hello everyone, If you can help, please respond directly to Dr. Charles Keith, chkeith-at-cb.uga.edu
His request is as follows: He is looking for someone who would be able to repair a Zeiss plan neofluor 40X - 160 mm tube length. The front seal has fallen out and the lens element seems OK. Thanks in advance for the assistance. john Contact: Dr. Charles Keith, chkeith-at-cb.uga.edu
******************************************** John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu
High Resolution Scanning Electron Microscopist/Engineer
United Technologies Research Center is seeking an engineer to fill the HR-SEM operator/engineer position at the United Technologies Research Center in East Hartford, CT. This position will provide support to the United Technologies Corporation Business Unites including Pratt & Whitney, Carrier, Sikorsky, Hamilton Sundstrand and Otis Elevator. The SEM operator/engineer will be responsible to operate and fully utilize both the high-resolution secondary imaging and high-resolution back-scattering imaging capabilities offered by the HR-SEM, to characterize various surface coatings, advance materials including metals and ceramics, and to conduct failure analyses. The ability to recognize fracture modes and origins of fractures is strongly desired. The candidate should have experience with compositional analyses using EDS, being capable of doing qualitative and quantitative analyses and also be familiar with various probing tools such as mapping and line profile. Must be capable of making judgment on combinations of imaging and EDS methods to give the best characterization results. Good communication and interpersonal skills are essential. Experience with electron backscatter diffraction (EBSD) is a plus.
Qualified candidates will have BS in Materials Science or an equivalent discipline with a minimum of 2 years SEM experience. U.S. citizen or permanent resident required.
Please send your resume to Employment Opportunities, Code MATS-2050-9049, 411 Silver Lane, East Hartford, CT 06118 or e-mail employment-at-utrc.utc.com. An equal opportunity employer.
Hi: I am looking for a high voltage cable for a Hitachi HU11. If you have one laying around and would like to get rid of it please let me know. Peter Jordan
With detectors that now quote Fe55 resolution at 130 eV or below, what would the corresponding resolution be for F, assuming that one has a good digital pulse processor and one subtracts background?
-----Original Message----- } From: McCaffrey, John=20 Sent: Monday, November 01, 1999 9:37 AM To: 'Ian MacLaren'
Fellow Microscopists,
As often seems to happen, I don't pay enough attention to valuable responses to the list server unless I am dealing with that problem at that moment. I believe there have been several responses within the last year about typical recovery percentages for university microscopy labs, but I cannot seem to find those responses. If anyone in a university environment could respond with information about their recovery percentage/subsidy level, particularly for TEM laboratories, I would very much appreciate it.
Thank you for taking the time to consider this request.
Sincerely,
Mick Thomas ----------------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
HU 11 series are well into their pensions now but you may be lucky with regard to a cable.
An alternative is to make contact with a high voltage organisation in you= r area. High voltage is not a problem that we should worry about. All electricty lines are high voltage, x-ray sets use high voltage etc etc. = I am sure if you look around in your local area you could find a company th= at will re make your old cable ends into a new cable.
Good luck
Steve Chapman
Senior Consultant E.M. Protrain, 6 Hillcrest Way, Buckingham Industrial Park, Buckingham MK18 1F= U, England Tel 44 (0)1280 814774 Fax 44 (0)1280 814007 E-mail - protrain-at-emcourses.com Web Site - http://www.emcourses.com For Consultancy and Courses in Electron Microscopy World Wide Courses available in - Australia, Canada, Europe, South Africa, New Zealand, Taiwan, United States, United Kingdom
I was recently asked by someone at a localy if I could make slides for a Wentzscope. From what I gather it is a transmitted light microscope with a large viewing screen. This will be for a hands-on exhibit. My question is, what is the best way or most typical way to preserve the specimen. It will probably undergo alot of handeling. I have epoxy resins that I use for petrographic thin sections which comes to mind first. Thanks. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif ____________________________________________
Would you please send this to the microscopy board on my behalf and forward replies. Thanks. ----------------------------------------------------------------------------
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I forgot an important detail -- this only works if you choose the US keyboard. After your note, I discovered that the recipe won't work on any of the Canadian keyboards, the French one, or the Swedish one - the rest I didn't check. So:
(new) Instructions for negative Miller indices:
* Choose the US keyboard. You can do this by holding down the mouse button on the flag that appears in the upper right of your screen (the keyboard choice), choose 'About keyboards . . .', choose 'Customize Menu', then choosing the US keyboard. Close the box, then go back to the flag and choose the US keyboard. * Choose the Symbol font * Hit the tilde (~) accent grave (`) key -- the key in the far upper left of the keyboard, not counting the row including the Escape key and Function keys -- to produce the bar above the numeral. * Hit the desired numeral, which should now appear below the Miller bar.
Cheers John
-----Original Message----- } From: Rouviere [mailto:jrouviere-at-cea.fr] Sent: Wednesday, November 03, 1999 3:12 AM To: McCaffrey, John
Greetings, I have just uploaded to the MAS Web Site=20 (http://www.microanalysis.org) the "Call for Papers & Registration=20 Information" for the International Union of Microbeam Analysis=20 Societies 2000 meeting in Kona Hawaii. The info can be found at:=20 http://www.microanalysis.org/iumas2000. The meeting is due to run=20 from the 9th to the 14th of July 2000.
Comments, Questions, Bs and Ms to yours truly John Mansfield.
Please note new FAX number.
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 (Leaving a phone message at 936-3352 is preferable to 358-7555) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hello,
I was recently asked by someone at a localy if I could make slides for a Wentzscope. From what I gather it is a transmitted light microscope with a large viewing screen. This will be for a hands-on exhibit. My question is, what is the best way or most typical way to preserve the specimen. It will probably undergo alot of handeling. I have epoxy resins that I use for petrographic thin sections which comes to mind first. Thanks. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif ____________________________________________
We (Liberty Science Center, Jersey City, NJ) use several Wentzscopes in our exhibits. We use commercially prepared slides (things like a bee sting, mosquito face, insect wing) and depend on our design and production department to set them up in a way that is more or less foolproof. You could get a lot more information from the inventor, and retailer, of the Wentzscope, Bud Wentz in oakland CA, 510-531-1214, or from the science center listserv. Instructions for getting on and leaving a message are on the website. http://www.astc.org.
This message is being posted on behalf of a colleague of mine. She recently inherited a Reichert Histostat Model 855 "cryostat microtome" (cryostat), and she is looking for a way to adapt the knife holder for disposable blades.
This particular cryostat has the knife holder built to one side (left side of the microtome), and it is clearly built to hold resharpenable steel knives. Because the knife holder is on the side, most of the disposable blade holder inserts are not usable because they clamp the disposable blade near the center of the insert. There is not enough room to slide the disposable blade holder toward the specimen and still clamp it securely in the knife holder.
Does anyone out there have this model of cryostat? If so, have you had any success using disposable blades with the cryostat? We are thinking there may be a third party supplier who makes a disposable blade holder that would work with the instrument.
Thanks for any assistance you can provide!
Bob Chiovetti GTI Microsystems rchiovetti-at-aol.com
Hi, I was wondering if anyone could advise me on whether it might be possible to to combine fluorescent antibody staining of bacteria (specifically Chlamydia trachomatis) with a cytochemical stain for glycogen? I am trying to count the number of infected cells/field and then score the inclusions for the presence or absence of glycogen. We currently stain using methanol/formalin to fix and Jone's iodine. To detect chlamydial inclusions we use a primary antibody to the major outer membrane protein with a FITC conjugated secondary antibody with Evan's blue to stain the cytoplasm of the cells non-specifically.
When processing difficult to embed specimens for TEM vacuum infiltration is often recommended for the resin infiltration steps. However, a lack of specfic technical detail seems to often accompany this recommendation.
Missing detail includes;
What pressure should be applied for vacuum infiltration ?
How long should this pressure be applied ?
Should the vacuum infiltration procedure be applied to the propylene oxide / resin steps and pure resin infiltration steps, or just to the pure resin infiltration steps ?
Can applying a vacuum be useful for any other of the processing steps, for instance, primary fixation ?
Is so, what pressure and how long ?
Many thanks in advanced for all responses.
Allan
------------------------------------------------------------ Allan Mitchell Technical Manager South Campus Electron Microscope Unit C/-Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand mailto:allan.mitchell-at-stonebow.otago.ac.nz
I realize that only a few labs out there are using this procedure, but = can anyone out there who is doing DGD (diethylene glycol distearate) = embedding recommend a source (and lot#) for successful embedding and = sectioning? So far we have not been able to obtain satisfactory = semi-thin sections with the recently purchased batches of DGD in the = last year. Has anyone had similar problems with recently purchase DGD?
Hank Adams Integrated Microscopy Core Molecular and Cellular Biology Baylor College of Medicine Houston, TX 77030
I am in the process of buying a new inverted scope and the decision is between Olympus and Nikon. These two manufacturers use different strategies for Nomarski (DIC) imaging. Olympus has a single nosepiece slider and multiple condenser prisms (tho they span more than 1 magnification, e.g., one prism for both 40x and 60x). Nikon goes the more conventional route with separate sliders for each objective. I intend to compare them side by side but I am interested in hearing comments from users and the optics gurus on whether either approach is superior in either practice or theory. For the record, I will be gettingboth a LWD condenser and a NA 1.4 condenser for whatever system I get. Most of our work will be at 60x (separate oil and water objectives) but I will the plan apo and fluor objectives at 10, 20, and 40 also. Thanks in advance for any comments.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
In the really old days, when Nikon scopes were black and small, they used a system called francion/yamamoto method of DIC, does anyone have info on how this worked or any parts for it? This can be for either transmitted or reflected light DIC. Please help! Ed Sharpe archivist for SMECC
} Subj: DIC (Nomarski) - Nikon vs Olympus } Date: 11/3/99 10:17:02 PM US Mountain Standard Time } From: PhillipsT-at-missouri.edu (Tom Phillips) } To: Microscopy-at-Sparc5.Microscopy.Com } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am in the process of buying a new inverted scope and the decision } is between Olympus and Nikon. These two manufacturers use different } strategies for Nomarski (DIC) imaging. Olympus has a single } nosepiece slider and multiple condenser prisms (tho they span more } than 1 magnification, e.g., one prism for both 40x and 60x). Nikon } goes the more conventional route with separate sliders for each } objective. I intend to compare them side by side but I am interested } in hearing comments from users and the optics gurus on whether either } approach is superior in either practice or theory. For the record, I } will be gettingboth a LWD condenser and a NA 1.4 condenser for } whatever system I get. Most of our work will be at 60x (separate oil } and water objectives) but I will the plan apo and fluor objectives at } 10, 20, and 40 also. Thanks in advance for any comments. } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211-7400 } (573)-882-4712 (voice) } (573)-882-0123 (fax) } }
The procedure described by "McCaffrey, John" does work on non-US keyboard= s if the "overstrike 'accent grave'" key is supported. So you should look f= or the (`) character, not the tilde (~). On a US keyboard, they are located = on the same physical key, but this is not a general rule. The standard accen= t grave or single quote character (') does not work for overstriking.
On a Belgian keyboard for example, you can find the "overstrike accent grave" immediately to the lower left of the return key. It is marked with= a pound sign, a mu (=B5) sign and an accent grave. You need to hold the 'Al= t Gr' key (right alt) together with this pound/mu key to produce the accent gra= ve. Then you press the space bar, producing an overstrike bar. Then type your Miller index. The cursor won't advance and the index will appear under th= e overstrike bar. Indeed, this (seemingly) only works with the symbol font = in MS programs like Word, PowerPoint etc. The procedure does not work in Cor= el Photopaint 8.0, where it produces adjacent symbols.
Best wishes
Siegfried Jaecques K.U Leuven - Dept. BMGO } } } Hello Jean-Luc, } } I forgot an important detail -- this only works if you choose the US } keyboard. After your note, I discovered that the recipe won't work on a= ny } of the Canadian keyboards, the French one, or the Swedish one - the rest= I } didn't check. So:
..snip... } } Cheers } John } } -----Original Message----- } } From: Rouviere [mailto:jrouviere-at-cea.fr] } Sent: Wednesday, November 03, 1999 3:12 AM } To: McCaffrey, John } Subject: Overstrikinkin revisited (negative Miller indices) } } } Dear John, } } I tried your recipe without success : } } } ------------------------ } } From: McCaffrey, John } Sent: Monday, November 01, 1999 9:37 AM } To: 'Ian MacLaren' } Subject: RE: Overstriking revisited } } } Hi Ian, } } } This works for both Macs and PC's. Use the 'Symbol" font, then } first type the lower or upper case tilde/accent key (~ and `) followed b= y } the number that you are interested: i.e., choose the SYMBOL font, then h= it } the tilde key followed by the appropriate numeral, say 2, to get `2. } ------------------- } } } In my word program this produce two adjactent symbols. How can I superpo= se } them ? } } } Best regards } } } } -- } } } ROUVIERE Jean-Luc
I am surprised : your new instruction does not work. There is still something different between the French and the US system (or microsoft word 98). I think that in a previous version of word (word 4 ?), superposition of two characters was allowed with a key combination.They do not seem to have kept this facility in the new word version. However, I am surprised that noone has made a font whith overstriked numbers ? I thought it was easy to create fonts. May be someone in the list knows how to make a new font with overstriked numbers ?
Cheers
Jean-Luc
--- in reply of :
Hello Jean-Luc,
I forgot an important detail -- this only works if you choose the US
keyboard. After your note, I discovered that the recipe won't work on any of the Canadian keyboards, the French one, or the Swedish one - the rest I didn't check. So:
(new) Instructions for negative Miller indices:
* Choose the US keyboard. You can do this by holding down the mouse button on the flag that appears in the upper right of your screen (the keyboard choice), choose 'About keyboards . . .', choose 'Customize Menu', then choosing the US keyboard. Close the box, then go back to the flag and choose the US keyboard. * Choose the Symbol font * Hit the tilde (~) accent grave (`) key -- the key in the far upper left of the keyboard, not counting the row including the Escape key and Function keys -- to produce the bar above the numeral. * Hit the desired numeral, which should now appear below the Miller bar.
Dear all, First of all, thanks to John McCaffrey for his efforts. Unfortunately, his suggestions haven't worked yet on my Mac.
I tried the procedure described by Siegfried Jacques on my Swedish Mac. I found the key he was describing and in Symbol it does indeed produce an overstrike. I still cannot get it to go over a number, though, they always appear sequentially. Maybe, this shortcut only works on a PC. It sounded like Siegfried was using a PC (by the reference to the "Alt Gr" key).
} I think that in a previous version of word (word 4 ?), superposition of t= wo } characters was allowed with a key combination. They do not seem to have } kept this facility in the new word version.
I agree with this statement of Jean-Luc Rouviere. I tried to say something similar before. There used to be some sequence of commands in Word which gave two superimposed characters (see a discussion on this group in June 1994, archived in Nestor's archive). I cannot seem to make this work in Word 98, although I am convinced from the help files that such functions are still in there somewhere, if a little difficult to use.
If there is someone out there who is a real whizz on Word for Mac, please advise the rest of us on how to superimpose two characters.
Hope we can all clear this one up soon.
Best wishes
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren Department of Experimental Physics Chalmers University of Technology S-412 96 G=F6teborg, Sweden Tel: +46 31 772 36 33 FAX: +46 31 772 32 24 email: ian.maclaren-at-physics.org Research Group Homepage: http://fy.chalmers.se/microscopy/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
N.B. 1 I leave Sweden on the 12th December for a Christmas holiday in England, answering email in this time will be slow. After the New Year I will be travelling to China.
N.B. 2 Please send email to ian.maclaren-at-physics.org instead of the fy.chalmers.se address from now on. This new address will redirect mail to whichever email service I am using, even after I have gone to China.
For MacOS, Word 98: http://support.microsoft.com/support/kb/articles/Q193/7/78.ASP
For Microsoft Word 2000: http://support.microsoft.com/support/kb/articles/Q211/6/42.ASP
For earlier Windows and Mac versions of Word: http://support.microsoft.com/support/kb/articles/Q193/7/77.ASP
Most of the methods for later versions of Word involve using the Equation Editor. The method of making a shortcut ("bar1") for the equation, as suggested by an earlier posting, sounds like a good one to me. Other keystroke methods may work in individual cases, but may not allow future versions of Word or Word on other platforms to show the characters correctly (not that following Microsoft's suggestions even guarantees that!).
Carl
On Thu, 4 Nov 1999, Ian MacLaren wrote:
: ------------------------------------------------------------------------ : The Microscopy ListServer -- Sponsor: The Microscopy Society of America : To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com : On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html : -----------------------------------------------------------------------. :=20 :=20 : Dear all, : First of all, thanks to John McCaffrey for his efforts. : Unfortunately, his suggestions haven't worked yet on my Mac. :=20 : I tried the procedure described by Siegfried Jacques on my Swedish : Mac. I found the key he was describing and in Symbol it does indeed : produce an overstrike. I still cannot get it to go over a number, : though, they always appear sequentially. Maybe, this shortcut only : works on a PC. It sounded like Siegfried was using a PC (by the : reference to the "Alt Gr" key). :=20 : } I think that in a previous version of word (word 4 ?), superposition of= two : } characters was allowed with a key combination. They do not seem to hav= e : } kept this facility in the new word version. :=20 : I agree with this statement of Jean-Luc Rouviere. I tried to say : something similar before. There used to be some sequence of commands : in Word which gave two superimposed characters (see a discussion on : this group in June 1994, archived in Nestor's archive). I cannot seem : to make this work in Word 98, although I am convinced from the help : files that such functions are still in there somewhere, if a little : difficult to use. :=20 : If there is someone out there who is a real whizz on Word for Mac, : please advise the rest of us on how to superimpose two characters. :=20 : Hope we can all clear this one up soon. :=20 : Best wishes :=20 : +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ : Ian MacLaren : Department of Experimental Physics : Chalmers University of Technology : S-412 96 G=F6teborg, Sweden : Tel: +46 31 772 36 33 FAX: +46 31 772 32 24 : email: ian.maclaren-at-physics.org : Research Group Homepage: http://fy.chalmers.se/microscopy/ : +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ :=20 : N.B. 1 I leave Sweden on the 12th December for a Christmas : holiday in England, answering email in this time will be slow. : After the New Year I will be travelling to China. :=20 : N.B. 2 Please send email to ian.maclaren-at-physics.org instead : of the fy.chalmers.se address from now on. This new address : will redirect mail to whichever email service I am using, even : after I have gone to China. :=20
Carl Henderson Electron Microbeam Analysis Laboratory University of Michigan=20 2501 C.C. Little Bldg. Ann Arbor, MI 48109-1063 USA -------------------------------- Voice: (734) 936-1550 FAX: (734) 763-4690 E-mail: chender-at-umich.edu --------------------------------
To All: The New England Society for Microscopy will hold it's annual Fall Symposium at Brandeis University, Waltham, MA on Wednesday, December 1, 1999. The meeting will run from 12 Noon to 9:30 pm. In addition to 3 invited speakers, in both the biological and material sciences, there will be a Poster Session open to both students and researchers. Prizes will be awarded for Best Poster, 2nd and 3rd place. There will be a cocktail reception, dinner, and after-dinner speaker following the annual business meeting. Advance registration is due by November 23rd. The registration fee for non-mem- bers is $45.00, $30.00 for NESM members, and reduced rates for students and retired members. The cost of the dinner is $25.00. (Note: Registration after November 23rd will NOT include dinner). Deadline for receipt of poster entries is also November 23, 1999. For further information and registration forms: contact Peggy Sherwood at e-mail: MESnesm-at-aol.com.
I am sorry for not giving a proper intro, DGD embedding is employed for = doing resinless section em. DGD allows ultrathin and semithin sectioning = of cytoskeletal and nuclear matrix preps for visualization of the = filamentous structure after the DGD is dissolved and removed in butanol. = See: Capco, et al., J Cell Biol. 98:1878-85 (1984) and Nickerson, et = al., Proc Natl. Acad. Sci. 87: 2259-2263 (1990).
Hank Adams Integrated Microscopy Core Molecular and Celllular Biology Baylor College of Medicine Houston, TX 77030
Your assumption is right on target re: one beam splitter for many objectives vs. individual beam splitters for each objective. The one beam splitter approach is usually optimized either for the lower mags or the upper mags (i.e., it will work great for 10x - 60x or great for 20x-100x). Since your preferred mags are in the center of both ranges, it should not be a problem. However, as with all microscopy, it is really best to test each system with your own application.
Just a reminder: check the ability of the compensator to provide really beautiful contrast when the background is tuned to soft dove gray. This is the most sensitive portion of the system and, if the sample is correctly prepared (i. e., a moderately thin sample with gradients), should give absolutely eye-popping results.
By the way: two quick references: There is a good discussion of DIC in "Optimizing Light Microscopy for Biological and Clinical Laboratories" (details available on the MME website). Also, for an extended discussion of how DIC works and how to optimize and interpret the results, see American Lab, April 1988, "Notes on the use of differential interference contrast in light microscopy", Pp. 96-100. (I can send you Xerox copies, if you are interested).
Good hunting! Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 05:57 PM 11/3/99 -0600, Tom Phillips wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear all, Thanks to Carl Henderson, I have the answer I was looking for. I used the information at http://support.microsoft.com/support/kb/articles/Q193/7/78.ASP and adapted it to getting bars over numbers. I used the overstrike character from symbol font, as already suggested by John Mansfield as the character for the top. To make the overbar high enough, I raised it by 2 points using the "Format Character" options.
I have produced a list of bar 1 to bar 6 using this technique and I have checked them. They have one advantage over those produced using Equation Editor. If you produce individual characters using equation editor (such as bar 1) and then insert this to make something like [10bar1] you find that the vertical alignment of the bar1 is different to the rest of the typed text. With this newly discovered method, they all have the same vertical alignment as ordinary text so nothing looks strange when they are inserted. I then used the suggestions of James Passmore and Su Sajip and made AutoCorrect entries for b1, b2 .. b6 which insert these formatted bar1 to bar6 characters. Finally, it may require a slight fiddling with the printing window settings to get them to print out right, it did on my Mac.
If you want to edit them later, just select Preferences } view } Show field codes, and you will see the code and can then change things.
The Word file with bar1 to bar6 is available from me if you want to save yourself some work. Just email me.
Thanks again for all the help and handy suggestions.
Best wishes
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren Department of Experimental Physics Chalmers University of Technology S-412 96 G=F6teborg, Sweden Tel: +46 31 772 36 33 FAX: +46 31 772 32 24 email: ian.maclaren-at-physics.org Research Group Homepage: http://fy.chalmers.se/microscopy/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
N.B. 1 I leave Sweden on the 12th December for a Christmas holiday in England, answering email in this time will be slow. After the New Year I will be travelling to China.
N.B. 2 Please send email to ian.maclaren-at-physics.org instead of the fy.chalmers.se address from now on. This new address will redirect mail to whichever email service I am using, even after I have gone to China.
At 03:57 PM 11/3/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am in the process of buying a new inverted scope and the decision is between Olympus and Nikon. These two manufacturers use different strategies for Nomarski (DIC) imaging. Olympus has a single nosepiece slider and multiple condenser prisms (tho they span more than 1 magnification, e.g., one prism for both 40x and 60x). Nikon goes the more conventional route with separate sliders for each objective. I intend to compare them side by side but I am interested in hearing comments from users and the optics gurus on whether either approach is superior in either practice or theory. For the record, I will be gettingboth a LWD condenser and a NA 1.4 condenser for whatever system I get. Most of our work will be at 60x (separate oil and water objectives) but I will the plan apo and fluor objectives at 10, 20, and 40 also. Thanks in advance for any comments. } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211-7400 } (573)-882-4712 (voice) } (573)-882-0123 (fax)
this topic and subject brings back horrific memories!
First off, I've never seen an LWD objective with an NA of 1.4 to match the NA of a 1.4 condenser. The only 1.4 NA condenser I have seen and use is the aplanatic model/design which is double oil immersion. With an Olympus 100X PlanAPO oil objective, it does a good job. These are not infinity corrected objectives. I found that the Olympus UPlanAPO for the BX series did not perform as well as the 160 length older objectives. So for BF, I use the 160 length PlanAPOs at all magnifications.... from 10X to 100X. I tried Zeiss PlanAPOs but was disappointed.
For phase, DIC and DF, I use UPlanFL Olympus flourites and am pleased with the results.
I've owned an Olympus IMT-2 inverted scope with LWD objectives hoping to get greater working distance and DOF. Didn't happen. Very poor results. While one can get increased working distance, the resolution just is not there. The NA of the objectives suffers as a consequence of the greater WD. I did not try an aplanatic condenser but if I recall, the intrinsic NA of the objectives did not exceed 1 at any magnification. Furthermore, after using the IMT-2 for about 6 months, I grew to hate it and promptly disposed of it.
Olympus has a new model out which hopefully is better. By all means check it out. I have owned and used Nikon scopes (none inverted) and found them to be totally deficient. The mechanics are poor and in my view, the objectives are inferior to any other maker's objectives. But they look pretty.
Zeiss, as ususual, makes a nice line of scopes. But since their implosion with reps and distributors, trying to get any info or help from Zeiss is near impossible. So I stay away from them.
Olympus and Nikon sales folks will generally give you good support and be responsive. This is good for customers. By all means, do try what Olympus and Nikon have these days but I'd encourage you to be very critical. Test each unit at the limits of what you expect to be doing and expect the instruments to provide. You may be unpleasantly surprised. But relieved.
Finally, the IMT-2 was made and sold by the hundreds. It was and probably still is a popular model for biotech work. It did not work for me since my use was high quality photographs. The IMT-2 did not deliver. If your application is different, the IMT-2 may work for you and save you some serious money. Used, they run about $7-8K with objectives, trinoc, stage, and condenser (non-phase). If you use the Olympus Universal condenser, each objective has a matching DIC prism, which is superior to systems that use a single prism in the nose piece and a separate analyzer (like BX series).
In spite of my LaB6 cathodes living their expected lifetimes, I still need to clean the wehnelt every 150-200 hours. The symptom which cleaning seems to remedy is beam stability after a specimen exchange. My gun is ion pumped and isolated ... when changing a specimen is exchanged via interlock, the chamber vacuum will come to normal in less than a minute, but beam current stability won't come back to normal 'till after 30minutes. Like I said, this symptom is minimized by cleaning deposits off the wehnelt near the emission aperture. My problem is this deposit is extremely difficult to remove ... I assume it is LaB, but I think the next time I have it out, I'll swap with the tungsten wehnelt and and examine this deposit with EDX. Assuming it is LaB depositing, is there a better way to remove it ... I'd love to dissolve it over night instead of using mechanical methods which can wear the stainless steel cap.
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
Dear Richard, My experience with vacuum infiltration is all with epoxy mounting in materials specimen prep., but it may be some help in your work. The purpose of vacuum infiltration is to get air out of the sample and use the resultant vacuum to suck resin into the fine spaces and holes in the material. However, all the materials being used in the emedding are liquids which will boil if the pressure is dropped too low. I use a vacuum dessicator with a three-way valve connected to an old vacuum pump I don't care much about. The vapors you will be puting into the pump are quite hard on it. I put the material in epoxy molds into the dessicator, which has a clear lid. Watching carefully , I start the pump and turn the valve to start sucking on the dessicator. You will see a bit of vapor appear, then some bubbles will come up to the surface of the epoxy. Then the epoxy will start to foam slightly. At this point I turn the valve to admit air. I usually repeat this three times. I would not use this technique on any liquid with a high vapour pressure or low boiling point, but it should work to get intimate contact between any liquid and solid. At 11:46 AM 11/4/99 +1300, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
A random thought on the subject which has absolutely nothing to do with the technical quality of either scope.
A consideration is how many users the scope has and how expert they are. If you are working with multiple users or limited experience, it is my experience that the simpler to use and adjust the better. It will save multiple hours of "fun" re-adjusting, or more correct trying to find the one thing someone changed.
Good luck on your decision.
Azriel Gorski
} Dear Dr. Phillips, } } Your assumption is right on target re: one beam splitter for many } objectives vs. individual beam splitters for each objective. The one beam } splitter approach is usually optimized either for the lower mags or the } upper mags (i.e., it will work great for 10x - 60x or great for 20x-100x). } Since your preferred mags are in the center of both ranges, it should not } be a problem. However, as with all microscopy, it is really best to test } each system with your own application. } } Just a reminder: check the ability of the compensator to provide really } beautiful contrast when the background is tuned to soft dove gray. This is } the most sensitive portion of the system and, if the sample is correctly } prepared (i. e., a moderately thin sample with gradients), should give } absolutely eye-popping results. } } By the way: two quick references: } There is a good discussion of DIC in "Optimizing Light Microscopy for } Biological and Clinical Laboratories" (details available on the MME } website). Also, for an extended discussion of how DIC works and how to } optimize and interpret the results, see American Lab, April 1988, "Notes on } the use of differential interference contrast in light microscopy", Pp. } 96-100. (I can send you Xerox copies, if you are interested). } } Good hunting! } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis. } } } At 05:57 PM 11/3/99 -0600, Tom Phillips wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
One of our facilities has an ISI SX-40A SEM which is no longer needed. This microscope is operational, but has been out of service. It needs to be moved to a good home. For additional information, please contact: Gary Troyer Ferro Corporation Diamonite Division 453 W. McConkey Street Shreve, OH 44676 330-567-2145 troyerg-at-ferro.com
David Gnizak Ferro Corporation Technical Center 7500 E. Pleasant Valley Rd. Independence, OH 44131 gnizak-at-ferro.com
} } } } } } I am in the process of buying a new inverted scope and the } decision is between Olympus and Nikon. These two manufacturers use } different strategies for Nomarski (DIC) imaging. Olympus has a } single nosepiece slider and multiple condenser prisms (tho they span } more than 1 magnification, e.g., one prism for both 40x and 60x). } Nikon goes the more conventional route with separate sliders for } each objective. I intend to compare them side by side but I am } interested in hearing comments from users and the optics gurus on } whether either approach is superior in either practice or theory. } For the record, I will be gettingboth a LWD condenser and a NA 1.4 } condenser for whatever system I get. Most of our work will be at } 60x (separate oil and water objectives) but I will the plan apo and } fluor objectives at 10, 20, and 40 also. Thanks in advance for any } comments.
} Do you have an inverted scope now and are looking for a new one? My } point is that } if you have not used an inverted scope, be very careful about what } you expect them } to do compared to a compound microscope. If you need high } resolution, a compound } scope is the only way to do this. An inverted scope will indeed } have greater WD } but at the expense of much lower resolution. } } Furthermore, why would you want to put PlanAPO or flourite objectives on an } inverted scope? You would be throwing money out the window. They } will not get } you any better performance. An inverted scope is basically useful } for low power, } fast examination of tissue and cell subjects. 10X, 20X objectives } and maybe a 40X } objective is about all you can expect from these systems. A new } Olympus IX will } run about $30K, and about the same for the Nikon. A Zeiss Axiovert } is more like } $50K. And if you added APO objectives, they would cost more and the sales } people would have a good laugh at your expense. Seriously, watch out. The } inverted scopes are fine for a specific niche use and if this is } your area, go for it. } If not, proceed with great caution. }
I must be missing something. Why would an inverted scope have lower resolution - assuming one used the same objectives and a high NA condenser. My inverted will be for a multi-user confocal system. We may buy 2inverteds so we can have another for a widefield fluorescence deconvolution system. Some clients want to use tissue culture plates, petri dishes, etc so I need the LWD capabilities of an inverted. But I don't get why that sacrifices high quality optical images of thin sections mounted on slides in a conventional manner. Obviously we need to use a 1.4 NA condenser for the best DIC but you can do that on an inverted without much problem (it is probably easier to oil the condenser on an inverted than an upright). I just got off the phone with a gentleman from Bioptics who pointed out that one thing to look for in choosing a scope with a hi NA condenser is that the size of the housing holding the condenser lens can be so big that it can impede getting the condenser close enough to a live cell perfusion chamber. That is an excellent point I had not considered. most of my live cell clients are using tissue culture plates and dishes and working at LWD and therefore lower condenser NA. The high NA condenser will be mostly for sectioned material on slides.
We routinely use our current Nikon Diaphot (inverted) with 60x oil and 60x water objectives and get great confocal images and expect our new confocal will greatly improve our transmitted light images.
I agree that an upright compound scope is easier to use and maintain. But I can think of no reason that buying PlanApo or fluorite objectives would be a cause for amusement. If there is some theoretical consideration that I have missed, I would sure like someone to explain it. I am planning on buying almost 20K worth of objectives for this scope and can't see why I don't want PlanApo and Fluor lenses.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
I am looking for an opinion from our vast wealth of knowledge and experiences.
I have a user who is collecting 'semaphore cactus' flower specimens during a field expedition to South America with limited lab access. She is going to start fixation (2.5% glut in Na Phosphate buffer) in the field, and continue processing upon her return to the USA. She will be in the field for 21 days, and she is looking for morphological data (not immuno) in LM, SEM, & TEM (obviously TEM is the sticking point here). Refrigeration (4 C) will be generally available.
We are looking for a recommendation on the best place to leave the tissue for upwards of 21 days, before continuing processing of the tissue:
a. leave in 2.5% glut Na. Phos buffer
b. rinse to Na. Phos buffer and hold
c. rinse to distilled water and hold.
Details:
Samples: Opuntia spinosissima (Cactaceae)
Flower samples: Ovules, anthers, styles & stigma
Why 2.5% glut in Na. Phosphate buffer?
Ans: Weight. Transport ampules of 25% Glut and pre-weighed mono- & dibasic sodium phosphate packets and mix with locally obtained distilled water (no pH'ing on site) and return only with samples in vials.
We are very willing to take any better solutions offered.
(I don't know she just wasn't willing to back pack in our hihg pressure freezing unit and some LN2 dewars :-)
Thanks!
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Supervisor 352 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu
We've been having a discussion around the MME office about ways to present microscopy information to larger audiences. PowerPoint has been the method of choice but our graphic artist has suggested that Flash might be a better alternative (more fonts, better graphics, more easily animated).
Have any of you had experience in these areas? If so, preferences?
You can answer me privately at mme-at-map.com
Many thanks! Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
We are going to be performing immunostaining on vibratome sections of rat cerebrum fixed in paraformaldehyde. I am working with an EM Corp H1200 Vibrating Microtome and have only been able to produce sections (40 micron) that contain "chatter" ( in the direction of the knife advance). I have tried varying the knife angle, the speed, the frequency with minimal improvement. The chatter is still present. My question: should I be able to produce chatter-free sections or do I have to work around this distortion of the section. I tried to contact EM Corp., but it appears they have gone out of business. Thank you in advance for any suggestions!
I would like to find out telephone number of The Bradford Research Institute or American Biologics or Dr. Robert W. Bradford who makes Bradford Variable Projection Microscopy System.
Bruce Russell does superb photomicrography, both still and video. He's just sent me this message:
} If you are in the mood, check out "Intimate Strangers" on PBS next Tuesday } and the three Tuesdays thereafter. I shot most of the micro-scenes for the } series (but please don't hold me responsible for the occasional } inappropriate choice of species--that's Hollywood).
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
I want to say thanks to all colleagues who was sent me their advice. I will replay personally to each of them. One again thanks you very much gentlemen.
Scientific Illustration Standards: I am working on a project requiring pen (illustrations) of various stages of plant tissues from gross morphology to paraffin embedded sections of tissues/cells and structures. I am looking for a reference of standards for this nature of botanical illustration. I have tried looking under plant anatomy and biological illustration, but there does not seem to be a book, or publication that gives these specifications (which are different than botanical illustration). Any ideas or suggestions are welcome. Thank you in advance. Please reply to my email: crow-at-aloha.net.
__________________________________________
M.H. Chapin PO Box 716 Lawai, Kauai, Hawaii 96765 email: crow-at-aloha.net
I have no particular knowledge of your specimens but I would store them in buffer. Microorganisms grow more frequently in phosphate buffers than in cacodylate so I would add sodium azide to the buffer.
Dave
On Thu, 4 Nov 1999 13:26:31 -0500 "Richard E. Edelmann" {edelmare-at-casmail.muohio.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am looking for an opinion from our vast wealth of knowledge and experiences. } } I have a user who is collecting 'semaphore cactus' flower specimens during a } field expedition to South America with limited lab access. She is going to start } fixation (2.5% glut in Na Phosphate buffer) in the field, and continue processing upon } her return to the USA. She will be in the field for 21 days, and she is looking for } morphological data (not immuno) in LM, SEM, & TEM (obviously TEM is the sticking } point here). Refrigeration (4 C) will be generally available. } } We are looking for a recommendation on the best place to leave the tissue for } upwards of 21 days, before continuing processing of the tissue: } } a. leave in 2.5% glut Na. Phos buffer } } b. rinse to Na. Phos buffer and hold } } c. rinse to distilled water and hold. } } } Details: } } Samples: Opuntia spinosissima (Cactaceae) } } Flower samples: Ovules, anthers, styles & stigma } } Why 2.5% glut in Na. Phosphate buffer? } } Ans: Weight. Transport ampules of 25% Glut and pre-weighed mono- & dibasic } sodium phosphate packets and mix with locally obtained distilled water (no pH'ing on } site) and return only with samples in vials. } } We are very willing to take any better solutions offered. } } } (I don't know she just wasn't willing to back pack in our hihg pressure freezing } unit and some LN2 dewars :-) } } Thanks! } } } } } } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Supervisor } 352 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } } "RAM disk is NOT an installation procedure." }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I am looking for an opinion from our vast wealth of knowledge and experiences. } } I have a user who is collecting 'semaphore cactus' flower specimens during a } field expedition to South America with limited lab access. She is going to start } fixation (2.5% glut in Na Phosphate buffer) in the field, and continue processing upon } her return to the USA. She will be in the field for 21 days, and she is looking for } morphological data (not immuno) in LM, SEM, & TEM (obviously TEM is the sticking } point here). Refrigeration (4 C) will be generally available. } } We are looking for a recommendation on the best place to leave the tissue for } upwards of 21 days, before continuing processing of the tissue: } } a. leave in 2.5% glut Na. Phos buffer } } b. rinse to Na. Phos buffer and hold } } c. rinse to distilled water and hold. } } Details: } } Samples: Opuntia spinosissima (Cactaceae) } } Flower samples: Ovules, anthers, styles & stigma } } Why 2.5% glut in Na. Phosphate buffer? } } Ans: Weight. Transport ampules of 25% Glut and pre-weighed mono- & dibasic } sodium phosphate packets and mix with locally obtained distilled water (no pH'ing on } site) and return only with samples in vials. } } We are very willing to take any better solutions offered. } } (I don't know she just wasn't willing to back pack in our hihg pressure freezing } unit and some LN2 dewars :-) } } Thanks! } } } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Supervisor } 352 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } } "RAM disk is NOT an installation procedure."
Why not include an ampoule or two of OsO4 in the kit (talk to the suppliers and find out if it might not be easier to have it shipped directly south). If the material is post fixed and rinsed, it should keep in dH20 for quite a while, especially at reduced temperature.
A second suggestion is to include some form of vacuum system. Generally I found that flowers and other aerial organs tended to have quite a volume on entrained air in them. This can cause problems in fixation and resin infiltration if not removed. I've had the best results removing it during the primary fixation step. A simple, and effective, packable vacuum system for exhausting this can be made from an ordinary hand-operated brake bleeding device available at any local auto parts store.
cheers, John Heckman Department of Materials Science and Mechanics Michigan State University
Members of MSA,MAS and x-ray microanalysts will be sad to hear that Ted Hall died in Cambridge a few days ago. The funeral will be at the Cambridge Crematorium on Thursday November 11th at 3.45pm (GMT). Ted will be remembered for many things and not the least for the Continuum-Normalisation analytical algorithm which he developed for the quantitative x-ray microanalysis of thin sections of bio-organic materials.
Please forward this ad to anyone whom you feel might be interested. If anyone knows of other lists that might serve this audience (cell biology/EM or aquatic vascular plant ecology) please notify me directly.
TIA
Bob Wise
As seen in the 29 October issue of Science:
The Biology and Microbiology Department at the University of Wisconsin Oshkosh seeks two tenure-track Assistant Professors: (1) Specialty in cell biology and electron microscopy. Responsibilities: Teach electron microscopy; share teaching cellular and molecular biology, introductory biology; develop a research program in cell biology; run and maintain well-equipped electron microscope facility (SEM and TEM); (2) Specialty in aquatic vascular plant ecology. Responsibilities: Share in teaching advanced ecology, botany, and introductory biology courses; develop a research program in aquatic ecology. Both positions begin 1 September 2000, and successful candidates will be expected to pursue extramural funding and supervise M.S. theses. Ph.D. required; postdoctoral and teaching experience desirable. Send letter of application, statements of teaching philosophy and research interests, curriculum vitae, reprints, three letters of recommendation, and transcripts to: Chair, Department of Biology and Microbiology, University of Wisconsin Oshkosh, Oshkosh, WI 54901 by 10 January 2000. For additional information, see website: http://www.uwosh.edu/departments/biology/. The University of Wisconsin Oshkosh is an Affirmative Action/Equal Opportunity Employer.
Dr. Robert R. Wise Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (920) 424-3404 wise-at-uwosh.edu http://www.uwosh.edu/departments/biology/wise/wise.html
At 08:31 AM 11/4/1999 -0800, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { GO GATORS Scott D. Whittaker 218 Carr Hall EM Technician Gainesville, FL 32610 University Of Florida ph 352-392-1184 ICBR EM Core Lab fax 352-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ The home of " Tips & Tricks "
Thanks for the new procedure! =20 As an added bit of strangeness, in Office98 for Mac, the 'US keyboard' trick doesn't work for fonts sizes 10 and 9. Go figure . . .
Cheers John
-----Original Message----- } From: Ian MacLaren [mailto:maclaren-at-fy.chalmers.se] Sent: Thursday, November 04, 1999 10:54 AM To: Carl Henderson Cc: Microscopy; YiMin Yao
Dear all, Thanks to Carl Henderson, I have the answer I was looking for. I used the information at http://support.microsoft.com/support/kb/articles/Q193/7/78.ASP and adapted it to getting bars over numbers. I used the overstrike character from symbol font, as already suggested by John Mansfield as the character for the top. To make the overbar high enough, I raised it by 2 points using the "Format Character" options.
I have produced a list of bar 1 to bar 6 using this technique and I have checked them. They have one advantage over those produced using Equation Editor. If you produce individual characters using equation editor (such as bar 1) and then insert this to make something like [10bar1] you find that the vertical alignment of the bar1 is different to the rest of the typed text. With this newly discovered method, they all have the same vertical alignment as ordinary text so nothing looks strange when they are inserted. I then used the suggestions of James Passmore and Su Sajip and made AutoCorrect entries for b1, b2 . b6 which insert these formatted bar1 to bar6 characters. Finally, it may require a slight fiddling with the printing window settings to get them to print out right, it did on my Mac.
If you want to edit them later, just select Preferences } view } Show field codes, and you will see the code and can then change things.
The Word file with bar1 to bar6 is available from me if you want to save yourself some work. Just email me.
Thanks again for all the help and handy suggestions.
Best wishes
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren Department of Experimental Physics Chalmers University of Technology S-412 96 G=F6teborg, Sweden Tel: +46 31 772 36 33 FAX: +46 31 772 32 24 email: ian.maclaren-at-physics.org Research Group Homepage: http://fy.chalmers.se/microscopy/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
N.B. 1 I leave Sweden on the 12th December for a Christmas holiday in England, answering email in this time will be slow. After the New Year I will be travelling to China.
N.B. 2 Please send email to ian.maclaren-at-physics.org instead of the fy.chalmers.se address from now on. This new address will redirect mail to whichever email service I am using, even after I have gone to China.
They are both good, but I prefer the Nikon design.....more flexible. -----Original Message----- } From: Tom Phillips {PhillipsT-at-missouri.edu} To: Microscopy-at-Sparc5.Microscopy.Com {Microscopy-at-Sparc5.Microscopy.Com}
Just one more minor point for those using the FIELD method in MS Word (I use Word 98 on a Mac) for creating negative Miller indices. In addition to adjusting the vertical position of the bar using FORMAT/FONT/Character Spacing, which Ian MacLaren stated, you can also align the two superimposed characters using the field array switch (\a). This switch is normally used to align characters in a column but I have found that the \ar, \ac and \al switches will work even if there is only one column in the array, i.e. the superimposed characters in the overstrike field equation. For example, I use the following field equation ...{EQ \o \ar(b,1)}...where b is the character that gives me a bar using the WP-MathExtendedA font (my own preference instead of the symbol character) and \ar is the switch that aligns the two superimposed characters to the right. This setting gives me the best looking output of a bar1 character set in my printed documents. For other font combinations, it may be that the \ac (center alignment) or \al (left alignment) give better results. Once you establish the switches that produce the best output, then definitely use the AUTOCORRECT feature (great suggestion James Passmore!) to assign names to the Miller indices, or what ever else you choose to combine.
My only other comment is be sure not to double click on the overstrike character set if you want to modify it,i.e. from bar1 to bar2, since Word will automatically start Equation Editor to make the changes. IMO this really messes things up since the overstrike characters are entered back into the document as an equation box (which is what we are trying to avoid). You must make changes to them as mentioned before by selecting Tools/Preferences/View/Show Field Codes, making the changes, and then unselecting the show field codes.
Thanks everyone for input on this topic and good luck.
David * * * * * * * * * * * * * * * David T. Hoelzer, Ph.D. Metals and Ceramics Division Oak Ridge National Laboratory Bldg. 5500, Mail Stop 6376 P. O. Box 2008 Oak Ridge, Tennessee 37830 * (865) 574-5096 {Work} * (865) 574-0641 {Fax} * Note: new area code (replaces old 423)
-----Original Message----- } From: Patton, David [mailto:David.Patton-at-uwe.ac.uk] Sent: Friday, November 05, 1999 3:06 AM To: edelmare-at-muohio.edu Cc: microscopy-at-sparc5.microscopy.com
I have no particular knowledge of your specimens but I would store them in buffer. Microorganisms grow more frequently in phosphate buffers than in cacodylate so I would add sodium azide to the buffer.
Dave
On Thu, 4 Nov 1999 13:26:31 -0500 "Richard E. Edelmann" {edelmare-at-casmail.muohio.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am looking for an opinion from our vast wealth of knowledge and experiences. } } I have a user who is collecting 'semaphore cactus' flower specimens during a } field expedition to South America with limited lab access. She is going to start } fixation (2.5% glut in Na Phosphate buffer) in the field, and continue processing upon } her return to the USA. She will be in the field for 21 days, and she is looking for } morphological data (not immuno) in LM, SEM, & TEM (obviously TEM is the sticking } point here). Refrigeration (4 C) will be generally available. } } We are looking for a recommendation on the best place to leave the tissue for } upwards of 21 days, before continuing processing of the tissue: } } a. leave in 2.5% glut Na. Phos buffer } } b. rinse to Na. Phos buffer and hold } } c. rinse to distilled water and hold. } } } Details: } } Samples: Opuntia spinosissima (Cactaceae) } } Flower samples: Ovules, anthers, styles & stigma } } Why 2.5% glut in Na. Phosphate buffer? } } Ans: Weight. Transport ampules of 25% Glut and pre-weighed mono- & dibasic } sodium phosphate packets and mix with locally obtained distilled water (no pH'ing on } site) and return only with samples in vials. } } We are very willing to take any better solutions offered. } } } (I don't know she just wasn't willing to back pack in our hihg pressure freezing } unit and some LN2 dewars :-) } } Thanks! } } } } } } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Supervisor } 352 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } } "RAM disk is NOT an installation procedure." }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Sorry to bother the US community, with this overstriking problem : on a French system, I still could not succeed in applying Siegfried's instructions... Mainly only voyels can be overstriked.
However, by browsing through the MS index help, I have finally succeeded in finding how to superpose two characters. I am not sure that this would be more convenient than the MS equation editor, but I mention it in reply to Ian MacLaren's query.
1) First, you have to insert a field (insert menu) 2) Then choose the kind of field. I have tried the equation field : EQ in the right menu 3) Then select the desired operator. For that, instead of typing the code, I have clicked on the option button and have chosen the \O( ) operator (O for overstriking I suppose) 4) Then, type the two characters you want to overstrike separated by ; (or may be comma in non French system) 5) You finally got something like : {EQ \O(1;?) } 6) Two modes are available for the fields : command or normal view. You can go back and forth between these two modes either with the preference box or by pressing the F9+option keys. On my computer (mac 7500), this is much faster than calling the MS equation editor.
Best wishes
PS. Up to now, I was using the MS equation editor to introduce overstriking. May be I will shift ? --
We want to view the surface waxes on leaves and stems of several plants (Brassica). We have previously viewed specimens using standard SEM and have prepared samples by standard methods. Currently we have access to an ESEM. Can anyone give us recommendations on conditions of sample preparation, temperature, humidity, pressure, and voltage that would be best suited for viewing surface waxes when using an ESEM?
The chatter has to be cause by movement between the knife and the speciman, the knife digging into the speciman or some kind of vibration in the knife, mount or machine..
Movement between the knife and specimen could be caused by warpage, a burr, wear or debris. Inspection and coating one of the mating surfaces with spotting compound such as Prussian blue and assembling and dissembling the parts will show if there is any problems with mating surfaces.
Most likely is the knife digging into the mount or the mount being deformed by the knife. You can try mounting in a different resin or use a knife with larger angle on the cutting edge and or a stiffer knife. Thinner or thicker sections might help.
I have had problems with chatter on everything I ever tried to cut at one time or another. Once something starts to chatter it is hard to get it stopped on that piece. Usually I can tinker with things until the chatter stops I would make up some dummy mounts to try different things until I found a combination that works.
Good luck Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger -----Original Message-----
} } We are going to be performing immunostaining on vibratome sections of rat } cerebrum fixed in paraformaldehyde. I am working with an EM Corp H1200 } Vibrating Microtome and have only been able to produce sections (40 micron) } that contain "chatter" ( in the direction of the knife advance). I have } tried varying the knife angle, the speed, the frequency with minimal } improvement. The chatter is still present. My question: should I be able } to produce chatter-free sections or do I have to work around this } distortion of the section. I tried to contact EM Corp., but it appears } they have gone out of business. Thank you in advance for any suggestions! } } Sandy Perkins
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Attention ListServer Members: RONTEC USA, Inc. is currently conducting a search for a "Product Manager" for its X-ray microanalysis group in Acton, Massachusetts. A description of this newly created position and information on the company may be seen at the RONTEC website (www.rontecusa.com) . If you are a US citizen meeting the job requirements and would care to apply for the position, please send your current resume' and a letter to: RONTEC USA 20 Main Street Acton, MA 01720 Attn.: Human Resources RONTEC USA is a subsidiary of the premier German EDX system manufacturer RONTEC AG. The growing RONTEC group specializes in EDX detectors and analyzers of advanced technology, microanalysis and X-ray imaging hardware and software for SEM, TEM and diffractometer applications. RONTEC is an equal opportunity employer.
I have recently installed an x-ray system with excellent digital imaging. I no longer need my passive digital imaging system that I purchased from GW Electronics, ($4900 for just the board). To install, one needs to hook up X-blanking, Y-blanking, and video (w/ annotation). The system is simple and acquires excellent images. I would be glad to send examples. The system has both visual (continuous scan) and record modes. The resolution of the image is a factor of the number of lines scanned by the SEM, (resolution is not limited by the GW board). Example, 2000 lines scanned with a normal landscape aspect ratio would result in 2500 pixels, this would mean a 5 megabyte image. The system acquires, saves and prints images. It has all the available formats tiff, jpg, gif, etc. It also has a built-in feature where it measures the line and frame periods of the SEM, so setup is a snap. Essentially you create a configuration file for each scan speed that you have. You open that file, click on start and you are acquiring images.
I am asking $2500 for just the board, $3100 for board w/ PC, I'll even install and train for $1250 + travel expenses. The PC has a writable CD, a 17 inch Sony trinitron monitor, and an EPSON 700 inkjet printer.
-----Oorspronkelijk bericht----- Van: Caroline Schooley {schooley-at-mcn.org} Aan: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com} Datum: samedi 16 octobre 1999 8:45 Onderwerp: Re: Help needed on slide preparation & staining techniques
} } Email: canew-at-jps.net } } Name: C. Newhouse } } School: Lowell HS } } } } Question: I am interested in finding a text or literature on } } slide preparation & staining techniques } } (& preparation of stains including Congo red, } } Safranin, Chrystal Violet, etc.) } }
Somehow I can't find the original message...
Some other interesting works (who are currently out of print, but can perhaps be found second-hand or in a library) dealing with a lot of specimens, preparation techniques and staining methods are:
If there is someone out there who is a real whizz on Word for Mac, please advise the rest of us on how to superimpose two characters.
Hope we can all clear this one up soon.
Best wishes
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Ian MacLaren Department of Experimental Physics Chalmers University of Technology S-412 96 G=F6teborg, Sweden Tel: +46 31 772 36 33 FAX: +46 31 772 32 24 email: ian.maclaren-at-physics.org Research Group Homepage: http://fy.chalmers.se/microscopy/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} } } } } } } } } } } } } } } } } } } } } } I hope here is a recipe that should work for WSWord in general = independently of the platform and allow to avoid EquationEditor. It's = not trivial but one may define a macros and make this real "one stroke = solution".
1. type in one line ...... [h-kl-] .......
2. select "-", go to "Format-} Font-} Spacing", in "Shift" (or probably = "placement", I use not english version of Word) select "Up" and choose = about half of font size setting, then OK.
3. back in the text select "h", "Format-} Font-} Spacing", in "Spacing" = (?) choose "compressed" and again about half of font size, OK.
After some trials it should give you what you want. If one will use = "shift+arrows" conbination to select characters, sequence may be saved = as macros and used by pressing single key.
I would like to know if someone encourage to try this and probably = distribute the macros.
Any reason why nobody wants to use the Mac OS keycaps accessory?
This will very quickly show which key combinations produce the required results.
Another of those minor features which make Macs so easy to use:-)
regards
-- Larry Stoter 17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK email: LPS-at-teknesis.demon.co.uk Home Phone/Fax: +44 (0)1825 767967 Work Phone: +44 (0)1293 527733
I am interested in mounting various kinds of marine invertebrates from an aqueous or 70% alcohol solution. I am atracted to the claims for CMCP-10 and CMCP-9. I tried making a number of kinds of aqueous mountants some years ago, including PVA, Glycerol-Gelatin, actually quite a number, but none of them proved really good---my home cooked PVA mountant was close, but .... no cigar.
After some years away from this work, I want to try CMCP-10 or 9.
My interestes are broad ranging---
Sponge spicules after corrosion in bleach Crab larvae Minute worms Worm larvae Various kinds of spicules and worm bristles Nematocysts Small worms
Can I ask on this list whether anyone has come up with a good solution for water soluble mounts, from marine material?
Thank you,
Alan Davis
-- adavis-at-netpci.com
"An inviscid theory of flow renders the screw useless, but the need for one non-existent." ---Lord Raleigh
We have a Philips XL30 ESEM. I have not looked specifically for waxes but I would observe a leaf as follows.
Mount using double sided conductive tabs. Place a drop of water on part of the leaf (so you know this region was always wet during pumpdown. Pumpdown at 5 deg. C, and 6.8 Torr. Flood x4 and observe at the dew point ie 6.5 Torr. If eventually water condences on the leaf alter the pressure to say 6.3 Torr. Use the lowest kV you can. (I find it hard to get a reasonable amount of signal below 10 or 15kV).
Good luck
Dave
On Fri, 05 Nov 1999 14:53:42 -0500 Margaret Lynch {mlynch-at-emerald.tufts.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear colleagues, } } We want to view the surface waxes on leaves and stems of several plants } (Brassica). } We have previously viewed specimens using standard SEM and have prepared } samples by standard methods. Currently we have access to an ESEM. Can } anyone give us recommendations on conditions of sample preparation, } temperature, humidity, pressure, and voltage that would be best suited } for viewing surface waxes when using } an ESEM? } } Many thanks, } } Margaret Lynch } Tufts University } Medford, MA 01255 } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Some chatter is endemic to vibratome sections due to the flexibility of the sample. If your antibody allows try hardening it a bit more with longer fixation or using freshly preparted paraformaldehyde, adding a little glutaraldehyde to the post-fix, or switching to methanolic Carnoy's. Surrounding the tissue with 2-4% low melting point agarose may help to immobilize it often helps.
My experience with vibratomes has been that mechanical sources of chatter will be in loosely clamped sample holder, sample coming loose from the holder, the blade clamp is either rusting out or someone has sprung it by opening too far, or the steel shaft of the advance mechanism and/or its brass sleeve have corroded.
Good luck, Glen
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
On Fri, 5 Nov 1999, Gordon Couger wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } The chatter has to be cause by movement between the knife and the } speciman, the knife digging into the speciman or some kind of vibration } in the knife, mount or machine.. } } Movement between the knife and specimen could be caused by warpage, } a burr, wear or debris. Inspection and coating one of the mating surfaces } with } spotting compound such as Prussian blue and assembling and dissembling } the parts will show if there is any problems with mating surfaces. } } Most likely is the knife digging into the mount or the mount being deformed } by the knife. You can try mounting in a different resin or use a knife with } larger angle on the cutting edge and or a stiffer knife. Thinner or thicker } sections might help. } } I have had problems with chatter on everything I ever tried to cut at one } time or another. Once something starts to chatter it is hard to get it } stopped } on that piece. Usually I can tinker with things until the chatter stops I } would } make up some dummy mounts to try different things until I found a } combination that works. } } Good luck } Gordon } } Gordon Couger gcouger-at-couger.com } } Stillwater, OK www.couger.com/gcouger } 405 624-2855 GMT -6:00 www.couger.com/gcouger } -----Original Message----- } } } } } We are going to be performing immunostaining on vibratome sections of rat } } cerebrum fixed in paraformaldehyde. I am working with an EM Corp H1200 } } Vibrating Microtome and have only been able to produce sections (40 micron) } } that contain "chatter" ( in the direction of the knife advance). I have } } tried varying the knife angle, the speed, the frequency with minimal } } improvement. The chatter is still present. My question: should I be able } } to produce chatter-free sections or do I have to work around this } } distortion of the section. I tried to contact EM Corp., but it appears } } they have gone out of business. Thank you in advance for any suggestions! } } } } Sandy Perkins } } } } }
The Department of Mechanical Engineering and Materials Science at Rice University seeks candidates for a postdoctoral research associate position to carry out research on the synthesis and characterization of multicomponent oxide thin films. The position includes research on the development of a new vacuum deposition tool. The ideal candidate should have demonstrated experience in advanced vapor phase deposition processes, either PVD or CVD, and in UHV systems. Experience in the areas of metal oxide thin film characterization (XRD, SEM, TEM and/or electrical measurements) is preferred. Candidates should hold a Ph.D. in Physics, Materials Science or a related field. The appointment is initially for one year, with a strong possibility of extension for a second year. Interested candidates should send a curriculum vitae, including publication list, and the names of at least three references with addresses and telephone numbers to: Prof. S. Stemmer, Department of Mechanical Engineering and Materials Science MS-321, Rice University, 6100 Main Street, Houston, TX 77005-1892. Email:stemmer-at-rice.edu. Rice University is an Affirmative Action/Equal Opportunity Employer.
We use conditions on an NSEM you could probably mimic on an XL-30. In our experience keeping the voltage as low as possible is very important to get an accurate picture of an uncoated wax surface. For 5kV, you will need to keep the working distance as low as possible, and the gas pressure to the minimum needed to reduce charging (around 1 torr?). Stabilise the leaf by keeping it cold - a Peltier or nitrogen-cooled stage if convenient, otherwise just place on a nitrogen-cooled metal block. You can probably get 5kV with water vapour under these conditions but if it condenses, helium should be fine.
cheers Sally Stowe
On Fri, 05 Nov 1999 14:53:42 -0500 Margaret Lynch {mlynch-at-emerald.tufts.edu} wrote:
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} -----------------------------------------------------------------------. } } } Dear colleagues, } } We want to view the surface waxes on leaves and stems of several plants } (Brassica). } We have previously viewed specimens using standard SEM and have prepared } samples by standard methods. Currently we have access to an ESEM. Can } anyone give us recommendations on conditions of sample preparation, } temperature, humidity, pressure, and voltage that would be best suited } for viewing surface waxes when using } an ESEM? } } Many thanks, } } Margaret Lynch } Tufts University } Medford, MA 01255 }
Dr Sally Stowe, Facility Coordinator Australian National University EM Unit Research School of Biological Sciences Box 475, ACT 2601, Canberra, Australia FAX 06 (0)2 6279 8525 http://www.anu.edu.au/EMU/home.htm
I too have an interest in LWD microscope objectives; consequently, I talked with the Olympus representatives who were pesent at the last MAS/MSA meeting, who told me they have a series of such lenses. I seem to have misplaced the literature they gave me, but as I recall they had WDs of about 20 mm and are available with 5X, 10X and 20X mags. (Tel: 516-488-3880; Fax: 516-222-7920)
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
I have come across a microscope of unusual design, made by AO in the USA, perhaps 40 years ago, identified as Busch Rathenow design. It has one eyepiece, but two parallel objectives within seperate tubes and individual "stages", or perhaps fixtures, as there is no conventional platform for glass slides. No condenser, so it appears to use surface illumination from its internal electrical light source. Does not seem to have been intended for microbiology. Objectives are mounted in two parallel tubes, via sliders, one objective per tube, both tubes incorporated in one stand with a common eyepiece. Not sure if this would be considered a useful instrument or collectible antique. Perhaps someone in the Society is familiar with this design and it's purpose?
I am a collector of scientific optical instruments.
WE feel we have solve all problems with storage of osmium tetroxide. Here is our laboratory=B4s procedure of handling and storage.
We make 4% osmiumtetroxide in 0.2M phosphatbuffer, and freeze it in small aliquotes =E1 2ml in blood sample glass (without any additives, except that it is silicone coated) with rubber top. The Osmium tetroxid will not enter trough glass or rubber. It enters trough plastic. In the freezer we store the glasses in double containers just to be shure. We don=B4t have blackening of even the inner container! (That=B4s because of the glass and the rubber stopper)
When we are using the osmium, we thaw one glass, and add 2 ml distilled water to make 2% in 0.1M buffer. We plase the blood sample glass in an erlenmeyer-beaker, and have aluminiumfoil around it to avoid light to destroy the osmium. The thawed glass are stored in a chemichal hood.
Good luck.=20
Gunnar and Nan Vennlig Hilsen=20 dr.ing Gunnar Kopstad overingeni=F8r Avd f Patologi, Rit
Thanks for the "Heads-Up" Caroline, will be watching tonite as I caught a = Preview last night on PBS.......very interesting! Mike Bucker Microscopist Principal Consolidated Labs of VA
} } } Caroline Schooley {schooley-at-mcn.org} 11/04 7:45 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
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Bruce Russell does superb photomicrography, both still and video. He's just sent me this message:
} If you are in the mood, check out "Intimate Strangers" on PBS next = Tuesday } and the three Tuesdays thereafter. I shot most of the micro-scenes for = the } series (but please don't hold me responsible for the occasional } inappropriate choice of species--that's Hollywood).
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html= =20 Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html=20=
After 15 years of successful typing our keyboard on Link AN-10000 EDS system (the rest of it works still very well) became unreliable (most employed keys are almost dead). From our local representative we got an offer for a new keyboard for a price high enough to by a new PC (including keyboard of course). I wonder if someone experienced similar problem and found a cheaper solution (such as to repair it, to exchange for a different type etc.).
The type of the keyboard is 1602-003.
Thank you for answers,
Goran Drazic J. Stefan Institute SI-1000 Ljubljana Slovenia
Greetings, Andre von Hoyer described a microscope (see below) that reminded me of a design that Leitz once made for doing interference microscopy. It was for transmitted (not incident) light and it had two microscopes in one. A single light source, split to shine through two matched condenser-objective systems and then reunited for the eyepiece. You put your sample in one and a reference slide in the other and could get interference between the sample and reference beam. Clearly this required lots of matched optics and careful alignment. Could it be that the scope designed below was meant to do intereference but with incident light? Just a wild guess.
Tobias Baskin
} } Sir- } } I have come across a microscope of unusual design, made by AO in the } USA, perhaps 40 years ago, identified as Busch Rathenow design. It has } one eyepiece, but two parallel objectives within seperate tubes and } individual "stages", or perhaps fixtures, as there is no conventional } platform for glass slides. No condenser, so it appears to use surface } illumination from its internal electrical light source. Does not seem to } have been intended for microbiology. Objectives are mounted in two } parallel tubes, via sliders, one objective per tube, both tubes } incorporated in one stand with a common eyepiece. Not sure if this } would be considered a useful instrument or collectible antique. Perhaps } someone in the Society is familiar with this design and it's purpose? } } I am a collector of scientific optical instruments. } } Andre von Hoyer } andre.vonhoyer-at-lmco.com
subscribe Microscopy {mishot-at-itsa.ucsf.edu} Larry D. Ackerman Lily & Yuh Nung Jan Laboratories Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Ave. San Francisco, CA 94143
March 15 -17, 2000 at the University of Central Florida, Orlando.
We will be offering a 3 day TEM specimen preparation short course that will include hands-on tripod polishing and FIB techniques at the University of Central Florida (sponsored by South Bay Technology and FEI company). This course will be offered the week of the joint meetings of the Florida AVS and Florida Society for Microscopy (an MSA local affiliate).
Instructors: Ron Anderson, IBM. Lucille Giannuzzi, UCF. Fred Stevie, Lucent Technologies
Additional information will follow. In you have questions, please contat:
Lucille Giannuzzi: lag-at-mail.ucf.edu
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
It sounds like a AO Spencer Direct Result Colorimeter. As taken from American Optical catalog ca. 1946, "It is applicable to all chemical and biological tests in which color density is a quantitative indication of composition." Basically, you zero the instrument with a standard using both plunger (objective) paths, then place the unknown in one plunger path and match the color between it and the side containing the standard using the independant knobs on either side. These knobs move the sample cups up and down. You apparently see a dividing line between the two fields in the eyepiece and can match color across this line. The depth difference between the two sides can then be read out as percent concentration of sample. Greg
Andre von Hoyer wrote:
} Sir- } } I have come across a microscope of unusual design, made by AO in the } USA, perhaps 40 years ago, identified as Busch Rathenow design. It has } one eyepiece, but two parallel objectives within seperate tubes and } individual "stages", or perhaps fixtures, as there is no conventional } platform for glass slides. No condenser, so it appears to use surface } illumination from its internal electrical light source. Does not seem to } have been intended for microbiology. Objectives are mounted in two } parallel tubes, via sliders, one objective per tube, both tubes } incorporated in one stand with a common eyepiece. Not sure if this } would be considered a useful instrument or collectible antique. Perhaps } someone in the Society is familiar with this design and it's purpose? } } I am a collector of scientific optical instruments. } } Andre von Hoyer } andre.vonhoyer-at-lmco.com
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
A good specification for F is 65eV. This makes the assumption that the detector is a Si(Li) and has a 129eV -at- MnKa specification. The type of pulse processor, assuming that it is a modern design will not degrade the F resolution. Both time variant and digital pulse processors provide similar resolution assuming they use the same time constant. Both Mn and F resolution should be measured on the electron column using real samples rather than Fe55.
David Rohde NORAN Instruments Inc.
DISCLAIMER: NORAN Instruments is a manufacturer of EDS detectors and systems.
-----Original Message----- } From: Ric Felten [mailto:smartech-at-javanet.com] Sent: Tuesday, November 02, 1999 7:26 AM To: Microscopy-at-sparc5.microscopy.com
Call the Frank Fryer Company, area code 847 in Illinois.....they should be able to help. -----Original Message----- } From: Andre von Hoyer {andre.vonhoyer-at-lmco.com} To: Microscopy-at-Sparc5.Microscopy.Com {Microscopy-at-Sparc5.Microscopy.Com}
i'd like to make a web-accessible instrument out of my LEO982. does anyone have a public domain script (java preferrably) for control of a SEM through a serial interface?
Brian McIntyre mailto:mcintyre-at-optics.rochester.edu Sr. Engineer lab: 716-275-3058/4875 River Campus EMLab fax: 716-244-4936 University of Rochester Rochester, NY 14620
"The most important thing a father can do for his children is to love their mother." - Unknown
We need to process synthetic vascular grafts made of porous polyurethane and dacron for light microscopy routine histology and image analysis and we need a resin that will not dissolve the PU and also allow immuno staining for endothelium and smooth muscle. We have had success with routine histo on the dacron grafts using Spurr Resin but all of the resins we have tried have dissolved the PU.
Can anyone suggest a resin or technique that will allow us to process our PU samples?
Thanks
Phil
Phillip Christopher Cardiovascular Research,UCT Anzio Road, Observatory, 7925 Cape Town, South Africa 27-21-4066613/6476(tel) 27-21-4485925(fax) ctschristopher-at-samiot.uct.ac.za
Saturday, April 21, Sunday, April 22, 2000 Saturday, April 28, Sunday, April 29, 2000
An advanced course on polarized light microscopy which will cover the following topics: The nature of polarized light The origin and interpretation of interference colors Birefringence and crystal orientation The Indicatrix Compensation and variable compensators Interference figures and their interpretation
The workshop will consist of two consecutive weekends of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy.
The course instructors include;
Jan Hinsch of Leica, Inc.,
John Reffner of Trace Consulting,
N.Y.M.S. Instructor Donald O'Leary.
WHEN: April 22, 23, 28 & 29, 2000 from 10 A.M. to 4 P.M.
WHERE: Location To be Announced.
COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership) Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Donald O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.
FURTHER INFORMATION: Contact Donald O'Leary
eMail: mailto:donoleary-at-worldnet.att.net
(201) 797-8849 Voice Phone Number
PLEASE POST ------------------------------------------------------------------------------------------------------------ Registration Form
Polarized Light Microscopy, April 22, 23, 28 & 29, 2000
For a Limited Time Only, we will provide you with FREE CONSULTATION SERVICE!
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To be removed from our list, simply click "reply" and put the words "Remove Debt Consolidation" in the subject line. Warning: If you do not put the words "Remove Debt Consolidation" in the subject line, you will not be removed. The process is automated.
I've got a sick piece of silicon, plastic and metal here. Just doing some testing. You should just trash this message without reading and there is NO need to reply....
Sorry for cluttering up your mailbox, but I need to test this section of the server.
I'm a phd student at INSA-Lyon and I'm working in Nitinol alloys in TEM and I'm writing to you because I'm having problems with electropolishing of NiTi for TEM observations. My problem is that when I do the electropolishing in the sample, I make holes in the sample perimeter I want to ask you what do you thiking about this problem and if do you know the right conditions to get a perfect thin foil? Thanks in advances for everything. Fadila Khelfaoui GEMPPM, UMR CNRS-INSA #5510 INSA de LYON F-69621 Villeurbanne cedex, France tel: (33) 4 72 43 84 14 post 50 51 fax: (33) 4 72 43 79 30 http://www.insa-lyon.fr/Laboratoires/GEMPPM/
Without an image of the scope, I would like to suggest Andre check to s= ee if the lens are water immersion . I seem to remember a microscope-like system which visually compared the= color of test solutions. I realize in this day of GC/MS and EDS and such, no= body does spot test or microchemical test, but there was a time when visual examination of color tests was required and special scopes were designe= d and used.
For my own edification, please contact me off line, who uses microchemi= cal or spot test and for what? I would really enjoy finding out.
Stay safe ................ frank (fskarl-at-goodyear.com)
BaskinT-at-missouri.edu on 11/09/99 05:40:23 PM To: Microscopy-at-Sparc5.Microscopy.Com -at- INTERNET cc:
I have quite a few old style Reichert OMU2/3 specimen chucks, the type with the square head screw that clamps the block. I don't have the complementary square head driver to turn the screw. My local hardware store guy just shrugged when I showed him the problem. Any ideas where I can find a tool to turn this screw?
Gary P. Radice gradice-at-richmond.edu Associate Professor of Biology 804 289 8107 (voice) University of Richmond 804 289 8233 (FAX) Richmond VA 23173 http://www.science.richmond.edu/~radice
I am working on a study of normal and wounded rat cornea. I have been having difficulty with "shrinkage" of the nuclei, mostly in the basal cell layer. I currently have been using a 1/2 strength Karnovsky's and have been playing with an amount of calcium chloride to try and reduce this "shrinkage". What I am in search of is a consistent fix for purely observing the ultrastructure of the rat cornea. I would appreciate any help you could offer. Thank you for your time.
Can anyone help me with finding protocols for localization of B-glucuronidase and carbonyl groups (separate procedures)?
I will be processing mouse cochlea through acetone to Epon-Araldite for 1-4 micron sections and need a protocol that will work after decalcification and stay throughout processing. I'm open to protocols that require aqueous processing (such as glycol methacrylate), but it will still be necessary to decalcify.
Replies may be sent directly to me.
Thank you for your assistance,
Jaclynn M. Lett, Research Assistant jmlett-at-cid.wustl.edu
Fay and Carl Simon Center for the Biology of Hearing and Deafness Central Institute for the Deaf 818 S. Euclid Ave. St. Louis, MO 63110
I would like to hear the replies also, so please post any responses to the list.
Robert S. Geske Research Associate Center for Comparative Medicine and Department of Pediatrics Baylor College of Medicine
-----Original Message----- } From: Jaci Lett [SMTP:jmlett-at-cid.wustl.edu] Sent: Wednesday, November 10, 1999 11:06 AM To: Microscopy Listserver; Histonet Listserver
I am resending this e-mail, hopefully for more responses.
} ---------- } From: Ingber, Bruce F.[SMTP:bingber-at-commserver.srrc.usda.gov] } Sent: Thursday, September 16, 1999 2:48 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: Equipment for disposition } } We will replace our primary SEM with a new ESEM in a few months. } Consequently, some of our older instrumentation will become available for } excess, surplus, or some other sort of disposition (spare parts?). Please } e-mail if there is there any interest for the following items? Government } agencies and universities have first priority for receiving excess or surplus property.
} 1. Cambridge S-250 SEM, was working well until CRT problem this past May } (horizontal line showing on screen). May be corrected for $5000-$8000 by } Leo, Inc. although some parts are not available any longer. Good for spare } parts. } } 2. Tracor-Northern 2010 EDS, still functional as of May, 1999. Replaced } CPU } board this past January. } } Bruce F. Ingber } Biologist- Electron Microscopy } USDA-ARS, SRRC } 1100 Robert E. Lee Blvd. } New Orleans, LA 70124-4305 } } (504) 286-4270; fax (504) 286-4419 } bingber-at-nola.srrc.usda.gov }
I'm sorry, this is way off-topic, but desperate situations call fro desperate remedies.
My wife, the only coffee drinker in the house, has a small Philips (Norelco in USA?) filter-type coffeemaker, "Cafe Duo", type HD 5190/C. Its virtue is its ability to make, quickly and easily, a single 200 ml cup of good coffee. Its nylon mesh filter (part number 482201570015) is starting to fall apart, and my local Philips office advises parts no longer available, even from Holland. I would be very grateful for the email or fax address of a spare-parts company, in any country, from whom I might stand a chance of sourcing this part.
thanks
Ritchie
Ritchie Sims Phone : 64 9 3737599 ext 7713 Department of Geology Fax : 64 9 3737435 The University of Auckland email : r.sims-at-auckland.ac.nz Private Bag 92019 Auckland New Zealand
We are selling a 15yr old Reichert Ultracut E ultramicrotome. It is in good condition, however it no longer will cut grey or bright silver sections. It is going to be used for immunocytochem studies. Silver sections or thinner sections will not be required. Does anyone have an idea how much it is worth?
Rick Felten-at-MACDERMID 11/10/99 11:20 AM I have acquired an old vacuum evaporator and I can only use the mechanical pump at this time. It can reach a 30-50 militorr region. Can I use this vacuum condition to clean Pt apertures?
Sorry All, but I'm still detecting problems and the only way I can check the DNS (Domain Name Server) resolver is to send this test message to the entire list. Looks like some sites are not getting Email while others are and I don't know for sure why. Unfortunately, sending Email to myself always works so I can't test it that way.
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We are cleaning out our shelves and want to try to sell a couple of hundred vacuum tubes of the types used in the power cabinets of the Philips EM-200 transmission EM. Many of the tubes have never been used but we also have some used ones from a number of old power cabinets in storage.
We would like to find out if there is still a market value for the tubes and where we should advertise them so they find a new owner rather than go to the local landfill. We would like to recoup some of the cost of the tubes if possible to help offset installation costs of some new equipment.
Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
To any one interested, Are ther any listers out there who still use the Siemans 1A. I remember it well. I gave me the best TEM micrographs I ever produced. If you do, and would like an exposure control module let me know. It's up for grabs (you cover the freight, 25 lbs from California) to the first person to contact me. It was given to me to pass on and no, I don't know if it works. Good luck, Stephen D'Angelo Equipment Resurrection Pacifica CA 650-738-0351
Hi, Does anyone have a protocol for the nuclear stain propidium iodide? Friends are working at a field station in Antarctica and that is the stain they have available for use (perhaps not the best choice but that's it). They are working on foraminifera. any help would be greatly appreciated. best regards, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
Hello Listers, I am in a jam and very much need schematic diagrams and service information for the Ziess/Leo EM10 and the EM900 Transmission Electron Microscopes. Hopefully, someone might have such information that I could borrow for just a couple days and would receive for their trouble some sort of remuneration on which we would agree. Please contact me off the List. Thanks. Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. PMB-499, 1807 West Slaughter Lane, Number 200 Austin, Texas 78748 Phone: 512/282-5507 FAX 512/280-0702
Hi, There was a listing in the last couple of days from a person at a company with a name like equipment resurrection. I deleted it by mistake and would appreciate having it resent. Thanks. Don Marshall
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology
"A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)
In our laboratory we have Edax 9100/40 detector with Be window. We are looking information (company in Europe, procedures, etc.) about replacement this window with Moxtek ultra thin window.
Henrik -- Henrik Kaker, Ph.D. SEM-EDS Laboratory Metal Ravne, Koroska cesta 14 Ravne, Slovenia, Phone: +386 602 21 131 Fax: +386 602 20 436 Mailto:Henrik.Kaker-at-guest.arnes.si http://www2.arnes.si/guest/sgszmera1/index.html
Would anyone care to trade or sell some parts I need? I am desparatly looking for a nose piece to fit a Zeiss Universal. Also an LKB NOVA ultramicrotome, operation manual copy and specimin holders. If you have any of these I can trade for something in my inventory or purchase them.
Folks: I need to replace a needle valve on an EMSCOPE CPD750 critical point dryer, it has a W on the body of the valve and the word Whitey on the knob, the OD of the copper feed pipe is 1/8th inch. Has anyone out there performed a similar repair? and if so where did you purchase the replacement valve Tx Simon
----------------------------------------- Simon C. Watkins Ph.D. MRCPath Associate Professor Director: Center for Biologic Imaging University of Pittsburgh Pittsburgh PA 15261 tel:412-648-3051 fax:412-648-8330 URL: http://sbic6.sbic.pitt.edu
I like to ask if anyone know the current phone number of Research and Manufacturing Company. Inc. in Tucson, Arizona. I want to order RMC cyrostat accessories from them. The number I have (602-889-7900) doesn't work now. Thank you.
******************************************************************* To see what is in front of one's nose requires a constant struggle.
George Orwell
Dorothy Zhang Harvard School of public Health Building 2, CVLAB 677 Huntington Ave, Boston, MA 02115 USA Phone# 617-432-6981 Fax# 617-432-2980
-----Original Message----- } From: Claudia Merson [mailto:Claudia.merson-at-yale.edu] {mailto:[mailto:Claudia.merson-at-yale.edu]} Sent: Friday, November 12, 1999 11:07 AM To: Hall, Ernest L (CRD)
Simon, You can get information on Whitey valves from the Whitey Company, 318 Bishop Road, Highland Heights, Ohio 44143. They don't put phone numbers in their general catalog because they rely on local reps. Whitey is a division of Swagelok Co. in Solon Ohio. We have used their valves for many years and can always get replacements. If you can't get a phone number elsewhere, call my local rep, Cambridge Valve and Fittings, 781-272-8270, and they should be able to help you. Good luck.
Don Marshall
} From Microscopy-request-at-sparc5.microscopy.com Fri Nov 12 10:39:41 1999 } } From: "simon watkins" {swatkins+-at-pitt.edu} } To: "microscopy" {microscopy-at-sparc5.microscopy.com} } Subject: needle valves } Date: Fri, 12 Nov 1999 10:13:54 -0500 } } } Folks: I need to replace a needle valve on an EMSCOPE CPD750 critical poin } dryer, it has a W on the body of the valve and the word Whitey on the knob } the OD of the copper feed pipe is 1/8th inch. Has anyone out there perform } a similar repair? and if so where did you purchase the replacement valve } Tx } Simon } } } ----------------------------------------- } Simon C. Watkins Ph.D. MRCPath } Associate Professor } Director: Center for Biologic Imaging } University of Pittsburgh } Pittsburgh PA 15261 } tel:412-648-3051 } fax:412-648-8330 } URL: http://sbic6.sbic.pitt.edu } }
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology
"A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)
Simon, You can get information on Whitey valves from the Whitey Company, 318 Bishop Road, Highland Heights, Ohio 44143. They don't put phone numbers in their general catalog because they rely on local reps. Whitey is a division of Swagelok Co. in Solon Ohio. We have used their valves for many years and can always get replacements. If you can't get a phone number elsewhere, call my local rep, Cambridge Valve and Fittings, 781-272-8270, and they should be able to help you. Good luck.
Don Marshall
} From Microscopy-request-at-sparc5.microscopy.com Fri Nov 12 10:39:41 1999 } } From: "simon watkins" {swatkins+-at-pitt.edu} } To: "microscopy" {microscopy-at-sparc5.microscopy.com} } Subject: needle valves } Date: Fri, 12 Nov 1999 10:13:54 -0500 } } } Folks: I need to replace a needle valve on an EMSCOPE CPD750 critical poin } dryer, it has a W on the body of the valve and the word Whitey on the knob } the OD of the copper feed pipe is 1/8th inch. Has anyone out there perform } a similar repair? and if so where did you purchase the replacement valve } Tx } Simon } } } ----------------------------------------- } Simon C. Watkins Ph.D. MRCPath } Associate Professor } Director: Center for Biologic Imaging } University of Pittsburgh } Pittsburgh PA 15261 } tel:412-648-3051 } fax:412-648-8330 } URL: http://sbic6.sbic.pitt.edu } }
Donald J. Marshall Relion Industries P.O. Box 12 Bedford, MA 01730 Ph: 781-275-4695 FAX: 781-271-0252 email dmrelion-at-world.std.com
Cathodoluminescence, mass spectroscopy, electron beam technology
"A weed is a flower out of place."
(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)
If you just want "contact sheets" of thumbnails for your notebook, Photoshop 5.0 and 5.5 has this capability under FILE: AUTOMATE: CONTACT SHEET . It is not as fancy as the commercial packages but it does a basic job.
} Hi Tony, } we've recently decided to get ThumbsPlus from Cerious software } (http://www.cerious.com/) its about 70 GBP and since its shareware you can } give it a go first. } } regards } } Ian } } } =========================== } Dr. Ian Hopkinson } Cavendish Laboratory, } Madingley Road } Cambridge. } CB3 0HE } ENGLAND } Tel: +44 (0) 1223 337 012 } fax: +44 (0) 1233 337 000 } =========================== } } On Fri, 12 Nov 1999, Anthony Moss wrote: } } } Dear Colleagues: } } } } I just received an advertisement for ImageAXS Pro, a program by Caere } } for keeping track of image libraries. It looks very interesting. They } } have a special deal on it 'til 15 Nov. I wondered what experiences } } people have had with this product - would you buy it again; are there } } any good alternatives. My platform is PC/Windows 95/98. Thanks very } } much in advance for your time. } } } } Sincerely, } } } } Tony Moss } } } } -- } } Dr. Anthony G. Moss } } Associate Professor } } Biological Sciences } } 131 Cary Hall } } Auburn University } } Auburn, AL 36849-5414 } } } } T: (334)844-9257 } } F: (334)844-4065 } } email: mossant-at-mail.auburn.edu } } http://www.auburn.edu/~mossant } }
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
We're trying to put together a Visual Basic program that will take a series of images from our SEM and display them in a thumbnail form. We'd also like to automatically compile them into an animation. Does anyone know of any VB components that already do this?
I am interested in height measurements from stereo pairs of images from SEM. I am aware of the Mex software by Alicona.
I seek comment/recommendation on same for PC or MAC, including comment on acuraccy and standards if possible.
Price data would be appreciated.
Thank you all in advance Brendan J. Griffin Centre for Microscopy and Microanalysis The University of Western Australia Nedlands, WA, AUSTRALIA 6907 ph 61-8-9380-2739 fax 61-8-9380-1087
INTERNATIONAL SYMPOSIUM ON MATHEMATICAL MORPHOLOGY
and its Applications to Image and Signal Processing
-------------------------------------------- June 26-28, 2000, Palo Alto, California, USA --------------------------------------------
This symposium is the fifth in a series of international workshops devoted to the area of mathematical morphology and its applications in image and signal processing. The scientific program will include invited talks and contributed papers. The size of the workshop will be limited in order to enable interaction between the participants.
Presentations on related areas will be welcome. Related topics of specific interest include, but are not limited to: * PDE methods * Discrete geometry for image analysis * Probabilistic methods * 3D image analysis * Scale space methods
SUBMISSION PROCEDURES: ----------------------
Prospective authors are invited to submit five copies of a full paper to the following address:
ISMM 2000 c/o Dan Bloomberg Information Sciences and Technologies Lab Xerox Palo Alto Research Center 3333 Coyote Hill Road Palo Alto, CA 94304 USA
Alternatively, email submissions will also be accepted. Manuscripts should be in PostScript or PDF format (if compressed, use gzip), and sent to the following address:
ismm2000-at-parc.xerox.com
Manuscripts should include a separate title page, containing authors names and contact information (including e-mail address, phone and fax number), an abstract of up to 200 words, and keywords.
Acceptance of papers will be based on appropriateness of the topic and on quality, novelty, and clarity of exposition. Each paper will be reviewed by at least two members of the Program Committee using a blind procedure. Their reviews will be returned to authors.
Accepted papers will appear in the proceedings published by Kluwer Academic Publishers, in their Computational Imaging and Vision series. This volume will be available at the beginning of the workshop. The final camera-ready papers must be prepared using the LaTeX document preparation system with the style files provided Kluwer Academic Publishers (these files will be available on the conference web site). They should not exceed 8 pages including artwork and references.
CONFERENCE SITE: ---------------
The conference will be held at the Xerox Palo Alto Research Center (PARC), one of the most prestigious research institutions in the United States. PARC is located on the western side of Palo Alto, 2 miles away from Stanford University, next to the Midpeninsula Foothills and their vast expanse of beautiful open space. It is within an hour of San Francisco, Santa Cruz, gorgeous Pacific beaches, regional parks with majestic redwood trees, nationally acclaimed wineries, and many other attractions. World famous sites such as Yosemite National Park, Napa Valley, and Lake Tahoe are only a few hours away. Closer to PARC, the City of Palo Alto also has a lot to offer and boasts a variety of gourmet restaurants and some fine shopping.
PARC researchers are credited for many of the innovations that led to modern day computing, including the bit-mapped graphical user interface, the laser printer, ethernet, etc. PARC is a vibrant institution, a cornerstone of Silicon Valley, and a place many look to for a glimpse at the future of computing.
Paper presentations will take place in the PARC Auditorium, which can accommodate up to 240 attendees. As part of the conference, a tour of the facilities will be arranged, along with demos and presentations by PARC researchers.
For additional information on PARC, visit http://www.parc.xerox.com/
IMPORTANT DATES: ----------------
November 30, 1999: Submission of full paper December 31, 1999: Notification of acceptance February 15, 2000: Camera-ready full paper
CONFERENCE CHAIRS: ------------------
Luc Vincent Dan S. Bloomberg Xerox Palo Alto Research Center Xerox Palo Alto Research Center 3333 Coyote Hill Road 3333 Coyote Hill Road Palo Alto, CA 94304 Palo Alto, CA 94304 USA USA
Banon , G.J.F, INPE, Brazil Barrera, J. University of Sao Paulo, Brazil Berman, M. CSIRO, Australia Boomgaard, R. van den University of Amsterdam, The Netherlands Dougherty, E.R. Texas A&M University, USA Heijmans, H.J.A.M. CWI, The Netherlands Kimia, B.B. Brown University, USA Kunt, M. EPFL, Switzerland Malladi, R. UC Berkeley, USA Maragos, P. National Technical University of Athens, Greece Meyer, F. Ecole des Mines, France Montanvert, A. Universite Pierre Mendes France, France Morel, J.M. ENS Cachan, France Roerdink, J.B.T.M University of Groningen, The Netherlands Ronse, C. Universite Louis Pasteur, France Salembier, Ph. UPC, Spain Schafer, R.W. Georgia Institute of Technology, USA Schmitt, M. Ecole des Mines, France Serra, J. Ecole des Mines, France
SPONSOR: -------
Xerox
INFORMATION: -----------
Please visit the official ISMM 2000 site at:
http://www.parc.xerox.com/ismm2000
This site will be updated regularly as more information becomes available. Information about registration, travel, accommodation, tourism, and invited speakers, will soon be available on this site. The final program will be published in January 2000.
At 07:18 PM 11/12/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Try Thumbs Plus from Cerious Software. http://www.cerious.com
They can catalog images. Animating them can be done in several ways, depending on exactly what you mean by that. There are several good GIF animation tools available.
} Hill Regional High School,a Public High School in New Haven CT has an } } educational partnership with Yale University...I am trying to locate } other High } Schools around the country who have Electron Microscopes in } their buildings and } are using them !) If any of your members are aware of } such schools I would be } grateful for the information.
Claudia -
One of the members of MSA's Education Committee, Steve Barlow {sbarlow-at-sunstroke.sdsu.edu} is assembling a list of EM-equipped high schools. He usually reads the listserver, so you may have heard from him already. There is one in a school in Tucson; the local Project MICRO chair, Gary Chandler {gwc-at-sem.Arizona.EDU} , can give you contact information. There is a SEM-in-a-van in Dayton, supervised by Carlton Bowers {c-bowers-at-usa.net} . Steve and I will be delighted to get input from other listserver readers.
Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
The Structural Virology Group in the department of Biological Sciences of Purdue University is looking for an electron microscopist. The individual should possess a BA/BS degree in one of the biological or physical sciences and have one year of experience past course work. For more information check our the website at: http://bilbo.bio.purdue.edu/~baker/ .
Norm Olson ******************************************* Norm Olson Senior Research Electron Microscopist Department of Biological Sciences Lilly Hall of Life Sciences Purdue University West Lafayette, IN 47907
Microscopists: Which labs out there have successfully used a network (internet or LAN) to transfer files from a commercially available EDS system? We plan to do that, and want to know what software/hardware has or hasn't worked. Please contact me off-line.
Yolande Berta School of Materials Science and Engineering Georgia Institute of Technology 404-894-2545 404-894-9140FAX yolande.berta-at-mse.gatech.edu
What is the purpose of connecting high schools with SEMs? I realize that this may seem like a dumb question but I'd like to know what the experiences of others have been in this regard.
First, do high schools that have SEMs or have access to SEMs differentiate themselves from those HSs that don't have SEMs? If so, what is the difference?
My HS in 1963 did have a SEM (TEM I guess) that I worked on nearly full time. But no one else cared about it but me. Admittedly, it was a technological and engineering challenge back then that no longer exists today.
I wonder what the statistics say about early exposure to SEM vs. career decisions, etc. From what I have seen, the SEM is not a get-rich avenue. Rather, software and engineering are much more lucrative. But the results that the SEM delivers and how it works is unquantifiable. There is no easy way to place a price on this. It may well be that an individual is stimulated by the SEM experience per se--no directly related job is at issue.
Having lectured and demonstrated at HS and colleges around my area, I am totally depressed by the broad disdain for science. This is not good at all. If the SEM is an avenue of exposure to science and math, and the fields that are closely related to these, I would appreciate hearing feedback about some success stories.
I've tried everything I know of to stimulate scientific interest in young people. All together, not very successfully. Maybe the SEM is a good vehicle. My SEM is mine....I can do with it what I choose. And I have many high schools in the locale.
Ironically, I was willing to donate a SEM to either of two local junior colleges. They rejected it because of too much burden to take it in and support it. I wound up selling the SEM for $9K. Something is really wrong with this picture. There must be some disconnect between the scholarly part of the educational faction and that of the fiscal controllers. I don't know.
I do know that there are too few young people interested in science. I don't think that this is a good scenario.
Frustrated...
gary g.
At 08:55 AM 11/13/99 , you wrote:
} } Hill Regional High School,a Public High School in New Haven CT has an } } } educational partnership with Yale University...I am trying to locate } } other High } Schools around the country who have Electron Microscopes in } } their buildings and } are using them !) If any of your members are aware of } } such schools I would be } grateful for the information. } } Claudia - } } One of the members of MSA's Education Committee, Steve Barlow } {sbarlow-at-sunstroke.sdsu.edu} is assembling a list of EM-equipped high } schools. He usually reads the listserver, so you may have heard from him } already. There is one in a school in Tucson; the local Project MICRO } chair, Gary Chandler {gwc-at-sem.Arizona.EDU} , can give you contact } information. There is a SEM-in-a-van in Dayton, supervised by Carlton } Bowers {c-bowers-at-usa.net} . Steve and I will be delighted to get input from } other listserver readers. } } Caroline } } } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html } Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html }
Ever try to contact someone for purchasing or service?
Undeliverable.
gary g.
} From: administrator-at-zeiss.com } Date: Sat, 13 Nov 1999 20:29:32 -0500 } Subject: Message not deliverable } To: "Dr. Gary Gaugler" {gary-at-gaugler.com} } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dorothy, Our area code in Tucson is now 520. Also, RMC was bought by Ventana Medical Systems. Doug
On Fri, 12 Nov 1999, Dorothy Zhang wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi everyone, } } I like to ask if anyone know the current phone number of Research and } Manufacturing Company. Inc. in Tucson, Arizona. I want to order RMC } cyrostat accessories from them. The number I have (602-889-7900) doesn't } work now. Thank you. } } ******************************************************************* } To see what is in front of one's nose requires a constant struggle. } } George Orwell } } } Dorothy Zhang } Harvard School of public Health } Building 2, CVLAB } 677 Huntington Ave, } Boston, MA 02115 USA } Phone# 617-432-6981 } Fax# 617-432-2980 } } ******************************************************************* } } } } }
---------------------- Douglas W Cromey, M.S. University of Arizona dcromey-at-U.Arizona.EDU
I have a Tecnai 20 TEM fabricated by Philips, and equipped with EDS and STEM. The microscope is operated by a software named TIA in stem mode, which Emispec wrote. Emispec also released additional software for drift correction which one could download on internet. I downloaded, and tried to use it, but it did not work. I even could not start the program.
Is there anybody who has an experience of using Drift Correction program for TIA? If so, could he or she tell me how to start the program and answer some questions that I have?
Best wishes,
Jondo Yun Department of Inorganic Materials Engineering Center for Instrumental Analysis Kyungnam University 449 Weolyeong-dong, Masan, 631-701, Korea 82-551-249-2697 (tel) 82-551-248-5033 (fax) jdyun-at-hanma.kyungnam.ac.kr
Is there someone in the Portland area who would like to help present a school workshop using the "Microscopic Explorations" manual? Here's the request; please reply directly to jfitter-at-teleport.com, with a copy to me. No experience is necessary, and you'll have a great time!
} No reply from ------. .... it would be great if we } could get a graduate student or a technician who uses a microscope to visit } us. We are planning the "Microscopic Explorations" for December. } } We are planning a video conference Remote Microscopy class with Dr. Kain. } (Dana G. Dunkelberger {danad-at-biol.sc.edu} ) } } Jere
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
Dr. Gary Gaugler wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } What is the purpose of connecting high schools with SEMs? I } realize that this may seem like a dumb question but I'd like to } know what the experiences of others have been in this regard. } } First, do high schools that have SEMs or have access to SEMs } differentiate themselves from } those HSs that don't have SEMs? If so, what is the difference? } } My HS in 1963 did have a SEM (TEM I guess) that I worked on nearly } full time. But no one else cared about it but me. Admittedly, it } was a technological and engineering challenge back then that no longer } exists today. } } I wonder what the statistics say about early exposure to SEM vs. career } decisions, etc. From what I have seen, the SEM is not a get-rich avenue. } Rather, software and engineering are much more lucrative. But the results } that the SEM delivers and how it works is unquantifiable. There is no easy } way to place a price on this. It may well be that an individual is stimulated } by the SEM experience per se--no directly related job is at issue. } } Having lectured and demonstrated at HS and colleges around my area, I } am totally depressed by the broad disdain for science. This is not good at all. } If the SEM is an avenue of exposure to science and math, and the fields } that are closely related to these, I would appreciate hearing feedback } about some success stories. } } I've tried everything I know of to stimulate scientific interest in young } people. All together, not very successfully. Maybe the SEM is a good } vehicle. My SEM is mine....I can do with it what I choose. And I have many } high schools in the locale. } } Ironically, I was willing to donate a SEM to either of two local junior colleges. They } rejected it because of too much burden to take it in and support it. } I wound up selling the SEM for $9K. Something is really wrong with } this picture. There must be some disconnect between the scholarly part } of the educational faction and that of the fiscal controllers. I don't know. } } I do know that there are too few young people interested in science. } I don't think that this is a good scenario. } } Frustrated... } } gary g. } } At 08:55 AM 11/13/99 , you wrote: } } } } Hill Regional High School,a Public High School in New Haven CT has an } } } } educational partnership with Yale University...I am trying to locate } } } other High } Schools around the country who have Electron Microscopes in } } } their buildings and } are using them !) If any of your members are aware of } } } such schools I would be } grateful for the information. } } } } Claudia - } } } } One of the members of MSA's Education Committee, Steve Barlow } } {sbarlow-at-sunstroke.sdsu.edu} is assembling a list of EM-equipped high } } schools. He usually reads the listserver, so you may have heard from him } } already. There is one in a school in Tucson; the local Project MICRO } } chair, Gary Chandler {gwc-at-sem.Arizona.EDU} , can give you contact } } information. There is a SEM-in-a-van in Dayton, supervised by Carlton } } Bowers {c-bowers-at-usa.net} . Steve and I will be delighted to get input from } } other listserver readers. } } } } Caroline } } } } } } Caroline Schooley } } Project MICRO Coordinator } } Microscopy Society of America } } Box 117, 45301 Caspar Point Road } } Caspar, CA 95420 } } Phone/FAX (707)964-9460 } } Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html } } Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html } } Gary,
I agree that it's terrible that science is not given higher status, but about 8 years ago I offered my school district an SEM with service for at least as long as my kids were in the district (at that time I was looking at 14 years). Essentially, they said "$250/yr to buy filaments and stuff is too much money" and my suggestion of finding another business to underwrite the expense was ignored. The reality was that the people with the power to say yes or no were intimidated by the equipment, and my offers of lots of help with fitting it into the curriculum meant meddling by outsiders. This is generally not accepted by the public school system because"they" are the experts and "we" are mere stupid parents. Our school district even treats its own collegues as mere stupid parents when they show up in that mode.
If there could truly be competition between schools for students we would have far better schools.
Gary, get those high school students on your microscope. See who's interested in science fairs (I had several students make it to the state level and all got special awards from EMSA, obviously a few years ago)
Ken Converse owner Quality Images third party SEM service
Gary, Your question requires much more than an email answer, but you are on the right track when you suggest that the SEM may be used to interest students in science rather than provide them a job. I would suggest that you, and others concerned with science education, take a serious look at the NAS website: www.nas.edu/rise
At 05:21 PM 11/13/99 -0800, Dr. Gary Gaugler wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Gary Chandler Materials Science and Engineering University of Arizona Tucson, AZ 85721 (520) 621-6078 FAX 621-8059 gwc-at-sem.arizona.edu
Yes, we do quantitative evaluation of stereo pairs.
There are a number of factors that impact the height resolution one can get from those images. In essence there is a trigonometric function that relates the z-resolution to the x/y resolution. The numerical factor depends on a number of other parameters with the stereo angle being the most important one. For typical stereo angles of 6 - 10 degrees, the factor is of the order of .1 - .2. In other words, the height resolution is about 1/5th to 1/10th of the x/y resolution (for example: x/y resolution 1 micron/pixel -} height resolution about 5 to 10 microns). It is possible to improve this through sub-pixel operations.
Please contact me off-line if you need more information.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Dr. Gary Gaugler [mailto:gary-at-gaugler.com] Sent: Saturday, November 13, 1999 9:35 AM To: Brendan Griffin Cc: Michael Bode
Please be advised that all RMC products and services were entirely merged with Ventana Medical Systems, Inc., Tucson, Arizona, USA in October of 1998.
The area code for Tucson is now (520) and our web site is now part of Ventana Medical's web site (see below).
We apologize for any difficulty in locating us. For all pertinent information on the US Headquarters use the address on the signature below. For sales and service locations worldwide, please see our web site.
Steven W. Miller North American Sales Manager Microscopy Products Group Ventana Medical Systems, Inc. 3865 N. Business Center Dr. Tucson, AZ 85705 Tel: 520-887-2155 In US: 800-227-2155 Fax: 520-690-3580 Web Site: www.ventanamed.com
Commercial message: Ventana is a very fast growing technology company manufacturing sample preparation equipment for microscopy and Clinical Pathology with automated immunolabeling, special stains and in-situ hybridization. Ventana Medical Systems, Inc. is publically traded on the Nasdaq under symbol VMSI.
Dear Henrik, Your best bet is to contact Gresham Scientific Instruments in the UK. (http://www.gsi-link.co.uk). They specialize in service and repair of all makes of EDX detectors and have a standard price for replacing the Be window with a Moxtek thin window and providing an electron trap. This is necessary with thin window detectors. At 01:35 PM 11/12/99 +0100, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Can anyone tell the exactly what formvar and pioloform are? I'm interested in the chemical nature of both of these grid coatings.
Thanks
Rick
=================================================== Rick Webb Senior Research Officer Department of Microbiology and Parasitology and Centre for Microscopy and Microanalysis University of Queensland 4072 Australia
One of the best ways to get kids interested is to hire them to do chores around the lab. I don't know if you can do it in today's law suit crazy world but it works.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
} From: Gary Chandler {gwc-at-u.arizona.edu} To: Dr. Gary Gaugler {gary-at-gaugler.com} ; MSA listserver {Microscopy-at-Sparc5.Microscopy.Com} } Gary, } Your question requires much more than an email answer, but you are on the } right track when you suggest that the SEM may be used to interest students } in science rather than provide them a job. I would suggest that you, and } others concerned with science education, take a serious look at the NAS } website: www.nas.edu/rise } } At 05:21 PM 11/13/99 -0800, Dr. Gary Gaugler wrote: } } } } What is the purpose of connecting high schools with SEMs? I } } realize that this may seem like a dumb question but I'd like to } } know what the experiences of others have been in this regard. } } } } First, do high schools that have SEMs or have access to SEMs } } differentiate themselves from } } those HSs that don't have SEMs? If so, what is the difference? } } } } My HS in 1963 did have a SEM (TEM I guess) that I worked on nearly } } full time. But no one else cared about it but me. Admittedly, it } } was a technological and engineering challenge back then that no longer } } exists today. } } } } I wonder what the statistics say about early exposure to SEM vs. career } } decisions, etc. From what I have seen, the SEM is not a get-rich avenue. } } Rather, software and engineering are much more lucrative. But the results } } that the SEM delivers and how it works is unquantifiable. There is no easy } } way to place a price on this. It may well be that an individual is } stimulated } } by the SEM experience per se--no directly related job is at issue. } } } } Having lectured and demonstrated at HS and colleges around my area, I } } am totally depressed by the broad disdain for science. This is not good } at all. } } If the SEM is an avenue of exposure to science and math, and the fields } } that are closely related to these, I would appreciate hearing feedback } } about some success stories. } } } } I've tried everything I know of to stimulate scientific interest in young } } people. All together, not very successfully. Maybe the SEM is a good } } vehicle. My SEM is mine....I can do with it what I choose. And I have many } } high schools in the locale. } } } } Ironically, I was willing to donate a SEM to either of two local junior } colleges. They } } rejected it because of too much burden to take it in and support it. } } I wound up selling the SEM for $9K. Something is really wrong with } } this picture. There must be some disconnect between the scholarly part } } of the educational faction and that of the fiscal controllers. I don't know. } } } } I do know that there are too few young people interested in science. } } I don't think that this is a good scenario. } } } } Frustrated... } } } } gary g.
I was asked to send the message following as reply for your question:
"There is a software (named "STERECON") for 3D reconstruction by SEM stereoimages developed at the faculty of Geology of the Moscow State University, Russia. The source information for STERECON includes an SEM stereo pair in BMP, JPEG or TIFF formats, and some parameters (such as magnification, stereo angle etc.). STERECON is based on photogrammetric principals and unique stereomatching algorithms. The processing is applied for grayscale images of different sizes (up to 2048x2048x8bpp). The accuracy of the results depends on many factors, among them are the hardware errors of an SEM, peculiarities of microrelief under the study, image resolution etc. The ordinary error for test-object microrelief mesurements is less then 5% for SEM "Hitachi S-800" and image resolution of 1024x1024. The results of 3D reconstruction (a grid of heights) can be exported to comma separated values ASCII file for future processing with any other software. STERECON is a pure Win32 software and works on MS Windows 95/98/NT operating system.
For more information please contact sokolov-at-geol.msu.ru (Prof. Viatcheslav N. Sokolov, the head of the SEM lab).
Best regards, Viatcheslav N. Sokolov mailto:sokolov-at-geol.msu.ru "
} --------------------------------------------------------------------- --- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Regards.
Victor Sidorenko, ANTRON Co. Ltd., Moscow, Russia.
Rick Webb asked: ================================================= Can anyone tell the exactly what formvar and pioloform are? I'm interested in the chemical nature of both of these grid coatings. ================================================ Formvar® is a registered trade name, CAS #63450-15-7, and is described chemically as being "polyvinyl formal" resin.
Pioloform® is also a registered trade name, CAS #63148-65-2, and it is described as being "polyvinyl formal" resin.
More information about these two resins can be found on the SPI Supplies website, the address for which is given below.
Disclaimer: SPI Supplies offers these and other resins for use as TEM support films.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Folks: I need to replace a needle valve on an EMSCOPE CPD750 critical point dryer, it has a W on the body of the valve and the word Whitey on the knob, the OD of the copper feed pipe is 1/8th inch. Has anyone out there performed a similar repair? and if so where did you purchase the replacement valve Tx Simon
----------------------------------------- Simon C. Watkins Ph.D. MRCPath Associate Professor Director: Center for Biologic Imaging University of Pittsburgh Pittsburgh PA 15261 tel:412-648-3051 fax:412-648-8330 URL: http://sbic6.sbic.pitt.edu
Dear Simon,
We are the manufacturers of the CPD7501 (and its sucessor to the CPD750) and can supply valves and other spare parts. Our US agent, Energy Beam Sciences (sales-at-ebsciences.com) will be able to help. The part number is 356201010 on-off valve (Whitey, valve angled). To fit the replacement, undo the two nuts to release the copper tubing from the valve . Remove the old valve from the panel and replace with the new. Refit the tubing and tighten the nuts.
Your best bet for service, maintenance and modifications on EDX detectors is the company AWESS, located near Hamburg in Germany. They routinely service EDAX detectors, and have converted many detectors from Be to UTW and SUTW. They can also replace the old Si(Li) crystal with a modern EDAX Sapphire(TM) crystal for improved light element performance and S/N ratios.
} } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } In our laboratory we have Edax 9100/40 detector with Be window. We are looking } } information (company in Europe, procedures, etc.) about replacement this window with } } Moxtek ultra thin window. } } } } Henrik } } -- } } Henrik Kaker, Ph.D.
I have been requested to post this message to the Microscopy listserver. Please respond to Patrick directly.
} Rutgers University has developed a new training program aimed at } users of machine sources of ionizing radiation. } 95% of the } training material focuses on users of analytical X-ray equipment. } Here in New Jersey, EM's are not exempt from the dosimetry and } training requirements in the New Jersey Administrative Code. The } requirements are vague to say the least. } } My question: Does anyone out there (either in or out of NJ) require } workers who only use EM's to attend formal radiation safety } training of any kind? If yes, how detailed is the training? If no, } are/were there any regulatory hurdles to consider before you and/or } your Rad. Safety Comm. made this decision? } } Thank you in advance for your help. } Regards, } Patrick J. McDermott } } } } Patrick J. McDermott } Rutgers Envir. Health & Safety } Rutgers, The State University } 24 Street 1603 } Piscataway, NJ 08854-8036 } (p) 732-445-2550 (f) 732-445-3109 } e-mail: mcdermot-at-rehs.rutgers.edu
Louie Kerr Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
Can anyone recommend courses that are offered in digital imaging basics? As we move to a more digital lab, I find that I would like a firm foundation in knowing why certain choices are preferred for image capture and manipulation. I am getting bits and pieces through Photoshop and some of our confocal manuals....but would like to speed up the learning curve. Thanks, Linda Fox lfox1-at-wpo.it.luc.edu
What is the best way to image a quenched protein complex? One of my user's goals is to image quenched protein complexes. Then he wants to compare the fluorescence intensity of the quenched complex to a protein complex that has been dequenched by denaturation. The protein is tagged with Oregon Green and the complexes are supposed to be 0.5 micron-1.0 micron in diameter.
Here at Alcoa (near Pittsburgh, PA), everyone operating an EM has to take an X-ray safety course in the form of an interactive CD. The course has to be repeated every second year. The requirement to take the course is based on state regulations and Alcoa's environmental and health policies. I see the benefit of the course in creating awareness to the radiation issue, so that no one starts messing around with ports and windows, etc. and later says: oh, I didn't know... Hasso Weiland } a Alcoa Technical Center Alcoa Center, PA 15069 ACT 221-3133 } * 724 337-3133 } * 724 337-2044 } mailto:hasso.weiland-at-alcoa.com } } ---------- } From: Louie Kerr[SMTP:lkerr-at-mbl.edu] } Sent: Tuesday, November 16, 1999 7:35 AM } To: Microscopy-at-sparc5.microscopy.com } Cc: mcdermot-at-rehs.rutgers.edu } Subject: EM Radiation Safety Considerations } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have been requested to post this message to the Microscopy listserver. } Please respond to Patrick directly. } } } } Rutgers University has developed a new training program aimed at } } users of machine sources of ionizing radiation. } 95% of the } } training material focuses on users of analytical X-ray equipment. } } Here in New Jersey, EM's are not exempt from the dosimetry and } } training requirements in the New Jersey Administrative Code. The } } requirements are vague to say the least. } } } } My question: Does anyone out there (either in or out of NJ) require } } workers who only use EM's to attend formal radiation safety } } training of any kind? If yes, how detailed is the training? If no, } } are/were there any regulatory hurdles to consider before you and/or } } your Rad. Safety Comm. made this decision? } } } } Thank you in advance for your help. } } Regards, } } Patrick J. McDermott } } } } } } } } Patrick J. McDermott } } Rutgers Envir. Health & Safety } } Rutgers, The State University } } 24 Street 1603 } } Piscataway, NJ 08854-8036 } } (p) 732-445-2550 (f) 732-445-3109 } } e-mail: mcdermot-at-rehs.rutgers.edu } } Louie Kerr } Research and Education Support Coordinator } Marine Biological Laboratory } 7 MBL Street } Woods Hole, MA 02543 } 508-289-7273 } 508-540-6902 (FAX) } 508-292-0289 (Cell phone) } } VISIT OUR WEB SITE: } http://www.mbl.edu } } }
March 15 -17, 2000 at the University of Central Florida, Orlando.
We will be offering a 3 day TEM specimen preparation short course that will include hands-on tripod polishing and FIB techniques at the University of Central Florida (sponsored by South Bay Technology and FEI company). This course will be offered the week of the joint meetings of the Florida AVS and Florida Society for Microscopy (an MSA local affiliate).
Instructors: Ron Anderson, IBM. Lucille Giannuzzi, UCF. Fred Stevie, Lucent Technologies
Additional information will follow. In you have questions, please contact:
Lucille Giannuzzi: lag-at-mail.ucf.edu
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
We are in need of an analytical double tilt holder for a JEOL 2000FX
Please contact Lucille Giannuzzi by email: lag-at-mail.ucf.edu or by phone (407) 275-4354,5,6
Thanks.
******************************************************************* Lucille A. Giannuzzi, Ph.D.
Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng., University of Central Florida, PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu
Director, UCF/Cirent Materials Characterization Facility, 12443 Research Parkway, Suite 305 Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321 -------------------------------------------------------------------- "Good judgement comes from experience.
Experience comes from making bad judgement."
Mark Twain ********************************************************************
****This is a correction to my earlier message, sorry for the inconvenience. ...
Rick Webb asked: ================================================= Can anyone tell the exactly what formvar and pioloform are? I'm interested in the chemical nature of both of these grid coatings. ================================================ Formvar® is a registered trade name, CAS #63450-15-7, and is described chemically as being "polyvinyl formal" resin.
Pioloform® is also a registered trade name, CAS #63148-65-2, and it is described as being "polyvinyl formal" resin. { { { { {THIS SHOULD HAVE BEEN "polyvinyl butyral" resin.
More information about these two resins can be found on the SPI Supplies website, the address for which is given below.
Disclaimer: SPI Supplies offers these and other resins for use as TEM support films.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
I was about to ask the same question, except we have an MT-7, also in N. Cal (Silicon Valley). Thanks.
-Rob Plano
Robert J. Plano Staff Analyst, SPM Services Charles Evans & Associates/Surface Science Labs (650)962-8767, ext. 742 http://www.cea.com http://www.surface-science.com
-----Original Message----- } From: Hayes, Fred (FA) [mailto:fhayes-at-dow.com] Sent: Monday, November 15, 1999 4:36 AM To: 'microscopy-at-microscopy.com'
We have an MT2 microtome in N. Ca which needs minor service/PM. Can anyone recommend a contact? Thanks.
Fred A. Hayes The DOW Chemical Co Analytical Sciences, Microscopy 1897 bldg, E78 Midland, Michigan 48667 517-638-2203 517-638-6443 fax
MME offers all sorts of customized courses on microscopy and related imaging. We have a new consultant who can even integrate what you need for digital imaging with what you know about Photoshop.
Please let me know if we can be of service.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 07:33 AM 11/16/99 -0600, Linda Fox wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I routinely use tilt angles of 15-30 degrees. It is too high value for visual observation of a stereopair, but often it is the best choice angle in terms of accuracy of stereo measurements (as long as I do not have overlapping, I can increase angle). Since I perform stereo measurements not often, I did not by any special software. Microsoft Excel works just fine for me.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: Michael Bode [mailto:mb-at-soft-imaging.com] } Sent: Monday, November 15, 1999 11:05 AM } To: 'Microscopy-at-MSA.Microscopy.Com' } Subject: FW: software for height measurement from stereo pairs?? } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Yes, we do quantitative evaluation of stereo pairs. } } There are a number of factors that impact the height } resolution one can } get from those images. In essence there is a trigonometric } function that } relates the z-resolution to the x/y resolution. The numerical factor } depends on a number of other parameters with the stereo angle } being the } most important one. For typical stereo angles of 6 - 10 degrees, the } factor is of the order of .1 - .2. In other words, the height } resolution } is about 1/5th to 1/10th of the x/y resolution (for example: x/y } resolution 1 micron/pixel -} height resolution about 5 to 10 microns). } It is possible to improve this through sub-pixel operations. } } Please contact me off-line if you need more information. } } Michael } } Michael Bode, Ph.D. } Soft Imaging System Corp. } 1675 Carr St., #105N } Lakewood, CO 80215 } =================================== } phone: (888) FIND SIS } (303) 234-9270 } fax: (303) 234-9271 } email: mailto:info-at-soft-imaging.com } web: http://www.soft-imaging.com } =================================== } } } } -----Original Message----- } } From: Dr. Gary Gaugler [mailto:gary-at-gaugler.com] } Sent: Saturday, November 13, 1999 9:35 AM } To: Brendan Griffin } Cc: Michael Bode } Subject: Re: software for height measurement from stereo pairs?? } } } Try Soft-Imaging's analySIS. It has optional plug-in modules that } I believe can do this. } } gary g. } } } At 09:12 PM 11/12/99 , you wrote: } } ------------------------------------------------------------- } ---------- } - } } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------- } ---------- }
Replying to David and posting to list as he suggested.
gary g.
At 09:28 AM 11/16/99 , you wrote: } Gary } } Thanks for the response. I hope you post this on the newsgroup. } } David Goldstein } } "Dr. Gary Gaugler" wrote: } } } At 09:06 AM 11/16/99 , you wrote: } } } Gary } } } } } } I cannot believe you meant what you appeared to say in response to an elementary } } } school teacher remarking on how fascinated his students were looking at pond } } } water under a light microscope. You stated that there had been discussion on the } } } MSA list server about allowing high school students access to SEMs to encourage } } } an interest in science. That is fine but you then when on to say: "maybe a } } } [light microscope] is a good tool as well" and "anything is better than nothing". } } } } } } The implication is that maybe a light microscope is useful in that it is better } } } than nothing but not by much and certainly not compared to a SEM. While SEM } } } images are truly magnificent, have you ever seen a live, moving subject under a } } } SEM--kind of hard for organisms to live in a vacuum chamber. I suspect that } } } the what fascinated the elementary students were the organisms hustling and } } } bustling before their eyes. Indeed you don't have to be in elementary school to } } } be fascinated. I remember one protozoologist stating something to the effect } } } that he could "spend all day looking at those critters" under a light } } } microscope. Indeed if you are going to learn something about the life processes } } } of microorganisms, I think it is a good idea to look at them when they are alive. } } } } } } Perhaps I misread what you intended to say. } } } } } } David Goldstein } } } } No, you did not misread what I said. But I see your point. And it is well taken. } } I see that it sounds like if one does not have a SEM, well, a lowly LM will have to do. } } } } I was focused (pun) on the SEM as an avenue and had not considered the LM. } } I have SEM and LMs here. You are right about seeing rotifers bustling about. } } Also mosquito larvae under the stereo zoom. Lots to see with the LM. } } } } I have to admit that the specimen prep for SZ and most LM is much easier than } } for SEM. I have about 350 prepared LM slides covering just about everything, } } from Anthrax, cancer, blood, parasites, tissues, pollen, etc. They are all neat } } to see with the LM. } } } } Thanks for pointing this out. } } } } gary g.
Watch for the announcment of annual meeting of MIcroscopy&Microanalysis'00 (http://www.msa.microscopy.com) there are pre-meeting workshops there. I'm not sure if there will be one on digital imaging this year but there is usually alot in this area going on.
Nestor Your Friendly Neighborhood SysOp.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
================================================================== Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." so I bought a G3 Mac
Living in South Africa, we have the great disparity between the "haves" and "have nots" and education systems that were a bit unbalanced in the past, to put it nicely. } From this our society has backed a project to try and bring a small SEM to previously disadvantaged schools. Together with this goes a series of easy to read microscopy books and some posters on the applications of EM. The aim is to simply afford some of the younger kids the chance to actually get to see a "piece of science". It is very difficult to go into a school that hardly has a desk and chairs and motivate them on science by presenting a talk on the different faculties available at Universities. Using the SEM we can demonstrate the uses of EM in science and so really grab their attention.
OED (of Australia)sponsored us with their Image Slave PC image grabbing system ( free plug ) which allows the kids to see how PC's are used to "control" equipment and do real work rather than only for PC games.
So here in South Africa we have a number of well off schools who can get to an EM unit to see "science" for real but we have enough schools who can't. So taking a SEM to them helps to even things out a bit.
} From this we have not seen a sudden rush on applicants to the universities for the sciences and not IT subjects, but as we all know young kids are so easily influenced and we only hope that we have encouraged a few to at least look at this field of interest. We are trying to work on a project with some of the schools where the SEM could be left there for a week or two to allow then to make their own samples and then capture their own SEM images. Takes a bit more effort but I am sure will be worth it. So I am sure this sounds like one of these do good world health adverts, but it's the way we are taking EM top schools in this country. It has been interesting to hear that in some countries schools actually have their own systems WOW.
Thanks for you time
Luc Harmsen Anaspec, South Africa Technical support on microscopy. Tel + 27 (0) 11 476 3455 Fax + 27 (0) 11 476 7290 anaspec-at-icon.co.za
-----Original Message----- } From: Dr. Gary Gaugler [mailto:gary-at-gaugler.com] Sent: Sunday, November 14, 1999 3:21 AM To: MSA listserver
What is the purpose of connecting high schools with SEMs? I realize that this may seem like a dumb question but I'd like to know what the experiences of others have been in this regard.
First, do high schools that have SEMs or have access to SEMs differentiate themselves from those HSs that don't have SEMs? If so, what is the difference?
My HS in 1963 did have a SEM (TEM I guess) that I worked on nearly full time. But no one else cared about it but me. Admittedly, it was a technological and engineering challenge back then that no longer exists today.
I wonder what the statistics say about early exposure to SEM vs. career decisions, etc. From what I have seen, the SEM is not a get-rich avenue. Rather, software and engineering are much more lucrative. But the results that the SEM delivers and how it works is unquantifiable. There is no easy way to place a price on this. It may well be that an individual is stimulated by the SEM experience per se--no directly related job is at issue.
Having lectured and demonstrated at HS and colleges around my area, I am totally depressed by the broad disdain for science. This is not good at all. If the SEM is an avenue of exposure to science and math, and the fields that are closely related to these, I would appreciate hearing feedback about some success stories.
I've tried everything I know of to stimulate scientific interest in young people. All together, not very successfully. Maybe the SEM is a good vehicle. My SEM is mine....I can do with it what I choose. And I have many high schools in the locale.
Ironically, I was willing to donate a SEM to either of two local junior colleges. They rejected it because of too much burden to take it in and support it. I wound up selling the SEM for $9K. Something is really wrong with this picture. There must be some disconnect between the scholarly part of the educational faction and that of the fiscal controllers. I don't know.
I do know that there are too few young people interested in science. I don't think that this is a good scenario.
Frustrated...
gary g.
At 08:55 AM 11/13/99 , you wrote:
} } Hill Regional High School,a Public High School in New Haven CT has an } } } educational partnership with Yale University...I am trying to locate } } other High } Schools around the country who have Electron Microscopes in } } their buildings and } are using them !) If any of your members are aware of } } such schools I would be } grateful for the information. } } Claudia - } } One of the members of MSA's Education Committee, Steve Barlow } {sbarlow-at-sunstroke.sdsu.edu} is assembling a list of EM-equipped high } schools. He usually reads the listserver, so you may have heard from him } already. There is one in a school in Tucson; the local Project MICRO } chair, Gary Chandler {gwc-at-sem.Arizona.EDU} , can give you contact } information. There is a SEM-in-a-van in Dayton, supervised by Carlton } Bowers {c-bowers-at-usa.net} . Steve and I will be delighted to get input from } other listserver readers. } } Caroline } } } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html } Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html }
Hi all On a recent trip to Taiwan a client there showed me a Bausch and Lomb Nanolab 2100 SEM. He needed some spares for it. Does any one know who I can contact for assistance on this SEM. Thanks
Luc Harmsen Anaspec, South Africa Technical support on microscopy. Tel + 27 (0) 11 476 3455 Fax + 27 (0) 11 476 7290 anaspec-at-icon.co.za
We seek an individual who has demonstrated expertise in a variety of analytical techniques including, but not limited to, X-ray diffraction, X-ray fluorescence, AFM, ISS, ESCA, AES, EDS, SSMS, TOFMS, AAS and electron microscopy including SEM, FESEM, TEM, AEM, STEM. Specific application of cryo-ultratome techniques is required. Demonstrated experience with the application of these techniques, including the development of three-dimensional maps of the atomic composition of mixed silver halide emulsion grains is highly desirable. Experience should be demonstrated by a patent and/or publication record.
This person must have a Ph.D. in chemistry, physics or material science, with at least 5 yrs experience in the photographic industry being highly desired. The ideal person would also have a deep interest in working in photographic science, in improving existing film products, and inventing new products using state-of- the-art technology.
The position involves developing technology for product use, with a high level of interaction with product development teams.
Resumes can be sent electronically to GaroneL-at-Polaroid.com For more information, please contact: Lynne Garone Polaroid Corp. 1265 Main St. Waltham, Ma. 02451 781 386-1446 GaroneL-at-Polaroid.com
The question of the chemical nature of Formvar came up a year or so ago. At that time i picked the brains of all the polymer chemists I could find around here, and looked in all the polymer books I could lay my hands on, and finally concluded that it is probably rhe result of the reaction of poly-(vinyl alcohol) with formaldehyde: CH / \ --CH2-CH(OH)-CH2-CH(OH)-- + HCHO =} --CH2-CH CH-- poly-(vinyl alcohol) | | O O \ / CH2 This appears to be analogous to the reaction producing poly(vinyl butyral, which is discribed in most books on polymer chemistry (e.g. Polymer Chemistry by M. P. Stevens, Oxford U. Press, 1990, p. 302, ISBN 0-19-505759-7)
I don't know what pioloform is (are you sure you've spelled it right?), but it might be a polymer made by this same type of reaction, but using some other aldehyde.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
Hasso Weiland wrote: ================================================ Here at Alcoa (near Pittsburgh, PA), everyone operating an EM has to take an X-ray safety course in the form of an interactive CD. The course has to be repeated every second year. The requirement to take the course is based on state regulations and Alcoa's environmental and health policies. I see the benefit of the course in creating awareness to the radiation issue, so that no one starts messing around with ports and windows, etc. and later says: oh, I didn't know... Hasso Weiland ================================================= Could you be more specific about the Commonwealth of Pennsylvania regulations to which you made reference. Safety courses are always good to have but I was not aware that this was a legal requirement in Pennsylvania. Are there any specifications about the courses as to length, frequency, and whether some kind of test of ability is required at the end of the course? Where can someone get a copy of these regulations? Thanks for calling this to my (our) attention.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
WE feel we have solve all problems with storage of osmium tetroxide. Here is our laboratory=B4s procedure of handling and storage.
We make 4% osmiumtetroxide in 0.2M phosphatbuffer, and freeze it in small aliquotes =E1 2ml in blood sample glass (without any additives, except that it is silicone coated) with rubber top. The Osmium tetroxid will not enter trough glass or rubber. It enters trough plastic. In the freezer we store the glasses in double containers just to be shure. We don=B4t have blackening of even the inner container! (That=B4s because of the glass and the rubber stopper)
When we are using the osmium, we thaw one glass, and add 2 ml distilled water to make 2% in 0.1M buffer. We plase the blood sample glass in an erlenmeyer-beaker, and have aluminiumfoil around it to avoid light to destroy the osmium. The thawed glass are stored in a chemichal hood.
Good luck.=20
Gunnar and Nan Vennlig Hilsen=20 dr.ing Gunnar Kopstad overingeni=F8r Avd f Patologi, Rit
We need to process synthetic vascular grafts made of porous polyurethane and dacron for light microscopy routine histology and image analysis and we need a resin that will not dissolve the PU and also allow immuno staining for endothelium and smooth muscle. We have had success with routine histo on the dacron grafts using Spurr Resin but all of the resins we have tried have dissolved the PU.
Can anyone suggest a resin or technique that will allow us to process our PU samples?
Thanks
Phil
Phillip Christopher Cardiovascular Research,UCT Anzio Road, Observatory, 7925 Cape Town, South Africa 27-21-4066613/6476(tel) 27-21-4485925(fax) ctschristopher-at-samiot.uct.ac.za
I assume that there is a typo and pioloform is polyvinyl butyral and formvar is polyvinyl formal.
Can I ask while I am on-line about a comment that I read somewhere ab= out formvar? It said something like formvar contains a lot of sulphur and= so is less suitable for immuno-labelling because there is more backgroun= d. Can I assume that newer plastics such as butvar contain less and so a= re better?
Malcolm Haswell Electron Microscopy School of Sciences Fleming Building University of Sunderland SUNDERLAND SR1 3SD Tyne and Wear UK
Tel (0191) 515 2872 e-mail: malcolm.haswell-at-sunderland.ac.uk =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D "Garber, Charles A." wrote: } =20 } -------------------------------------------------------------------= ----- } The Microscopy ListServer -- Sponsor: The Microscopy Society of Ame= rica } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscop= y.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ= .html } -------------------------------------------------------------------= ----. } =20 } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } =20 } Rick Webb asked: } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D } Can anyone tell the exactly what formvar and pioloform are? I'm int= erested } in the chemical nature of both of these grid coatings. } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D } Formvar=AE is a registered trade name, CAS #63450-15-7, and is desc= ribed } chemically as being "polyvinyl formal" resin. } =20 } Pioloform=AE is also a registered trade name, CAS #63148-65-2, and = it is } described as being "polyvinyl formal" resin. } =20 } More information about these two resins can be found on the SPI Sup= plies } website, the address for which is given below. } =20 } Disclaimer: SPI Supplies offers these and other resins for use as = TEM } support films. } =20 } Chuck } =20 } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D } =20 } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President 1-800-2424-SPI } SPI SUPPLIES FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } =20 } Look for us! } ######################## } WWW: http://www.spi.cc } ######################## } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D
I had the same problem with some synthetic ligament tissue. I needed sections for TEM so the problem was even more challenging. Finally, I started using a fast setting epoxy (sets in 3-5 minutes) to minimize diffusion length. In some instances, I coated the material with C to again ensure that there was no diffusion into the sample. The blocks were just hard enough to section after 1 hr. but even better the following day. This epoxy was from Cole Parmer, but I know there are many other suitable ones available in Ted Pella etc...(probably more expensive). I tried others but found the fast setting epoxy to be just as effective and easiest to use.
Cheers,
Jennifer Taylor
Ph.D. Candidate Stevens Institute of Technology Hoboken, New Jersey 07030 phone: (201)216-8310 fax:(201)216-8306
On Tue, 16 Nov 1999, ctschristopher wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi everyone } } We need to process synthetic vascular grafts made of porous polyurethane and } dacron for light microscopy routine histology and image analysis and we need } a resin that will not dissolve the PU and also allow immuno staining for } endothelium and smooth muscle. We have had success with routine histo on the } dacron grafts using Spurr Resin but all of the resins we have tried have } dissolved the PU. } } Can anyone suggest a resin or technique that will allow us to process our PU } samples? } } Thanks } } Phil } } Phillip Christopher } Cardiovascular Research,UCT } Anzio Road, Observatory, 7925 } Cape Town, South Africa } 27-21-4066613/6476(tel) } 27-21-4485925(fax) } ctschristopher-at-samiot.uct.ac.za }
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Reply to: Re: Formvar and Pioloform Hi Malcolm,
I use formvar all the time for immunolabeling and have never had a problem = with it. =
Usually, if someone has lots of background over the formvar it can be = traced back to being either an antibody problem or crossreactivity of the = blocking solution with antibody.
Why complicate things by having to try out different plastics when formvar = works fine?
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
Malcolm Haswell wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.= Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html=
} } = } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } = } } Rick Webb asked: } } = =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D } } Can anyone tell the exactly what formvar and pioloform are? I'm = interested } } in the chemical nature of both of these grid coatings. } } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D } } Formvar=AE is a registered trade name, CAS #63450-15-7, and is = described } } chemically as being "polyvinyl formal" resin. } } = } } Pioloform=AE is also a registered trade name, CAS #63148-65-2, and it = is } } described as being "polyvinyl formal" resin. } } = } } More information about these two resins can be found on the SPI = Supplies } } website, the address for which is given below. } } = } } Disclaimer: SPI Supplies offers these and other resins for use as TEM } } support films. } } = } } Chuck } } = } } = =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D } } = } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } } President 1-800-2424-SPI } } SPI SUPPLIES FAX: 1-610-436-5755 } } PO BOX 656 e-mail:cgarber-at-2spi.com } } West Chester, PA 19381-0656 USA } } Cust.Service: spi2spi-at-2spi.com } } = } } Look for us! } } ######################## } } WWW: http://www.spi.cc } } ######################## } } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D } } } } } RFC822 header } ----------------------------------- } } Received: from Sparc5.Microscopy.Com [206.69.208.10] by mailhouse.hei.= org } (SMTPD32-4.07) id A45D2E110278; Wed, 17 Nov 1999 11:39:09 PST } Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.= 11) = } id EAA01434 for dist-Microscopy; Wed, 17 Nov 1999 04:24:45 -0600 } Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by = } Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id EAA01431 for = } "MicroscopyFilteredEmail-at-msa.microscopy.com"; Wed, 17 Nov 1999 04:24:14 -= 0600 } Received: from avon.sunderland.ac.uk (avon.sunderland.ac.uk [157.228.0.= 12]) by = } Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id EAA01424 for = } {microscopy-at-MSA.microscopy.com} ; Wed, 17 Nov 1999 04:23:59 -0600 } Received: from sunderland.ac.uk } (j-merchant-pc.sunderland.ac.uk [157.228.73.219]) } by avon.sunderland.ac.uk (Sun Internet Mail Server } sims.4.0.1999.09.28.17.31.p2) } with ESMTP id {0FLC0094E5DGQL-at-avon.sunderland.ac.uk} for } microscopy-at-MSA.microscopy.com; Wed, 17 Nov 1999 09:36:52 +0000 (GMT) } Date: Wed, 17 Nov 1999 09:36:30 +0000 } From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} } Subject: Re: Formvar and Pioloform } To: "Microscopy (MSA)" {microscopy-at-Sparc5.Microscopy.Com} } Message-id: {3832771E.E419156B-at-sunderland.ac.uk} } MIME-version: 1.0 } X-Mailer: Mozilla 4.6 [en] (Win95; I) } Content-type: text/plain; charset=3Diso-8859-1 } Content-transfer-encoding: QUOTED-PRINTABLE } X-Accept-Language: en } References: {199911160827.DAA11947-at-generic2.axs2000.net} } Errors-to: Microscopy-request-at-sparc5.microscopy.com } X-UIDL: 242866408 } Status: U } =
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
by saluki-mailsmtp.siu.edu (8.9.1/8.9.1) with ESMTP id PAA113860 for {Microscopy-at-sparc5.microscopy.com} ; Wed, 17 Nov 1999 15:14:36 -0600 Received: from [131.230.177.129] (ws177129.microscope.siu.edu [131.230.177.129]) by saluki-mail.siu.edu (8.9.1/8.9.1) with SMTP id PAA80178 for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Nov 1999 15:15:25 -0600 X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu Message-Id: {v02130503b458c9b32afa-at-[131.230.177.129]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
We are seriously considering purchasing a Pixera Pro camera for use on a Macintosh 8500. It will be used primarily for capturing images from light microscopes (stereomicrosopes and conventional LM's). We already have a low light digital camera for fluorescence work so speed of the camera is not really a concern.
Does anyone have experience with this camera? Good and bad experiences are welcome.
I had heard that they were about to come out with a higher resolution camera so we waited for over a year -- but nothing has materialized to date. Now, we really need to purchase a camera in that price range.
Vendors are welcome to contact me as well.
#################################################################### John J. Bozzola, Ph.D., Director Micro-Imaging and Analysis Center 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
{fontfamily} {param} Times {/param} {bigger} {bigger} MATERIALS CHARACTERIZATION FACILITY-DIRECTOR
THE UNIVERSITY OF CALIFORNIA, IRVINE, DEPARTMENT OF CHEMICAL AND BIOCHEMICAL ENGINEERING AND MATERIALS SCIENCE invites qualified applicants for the position of SENIOR DEVELOPMENT ENGINEER, beginning JANUARY 1, 2000. The position carries a salary in the $60,000-$70,000 per year range, depending on experience and qualifications.
Applicants must have a Ph.D. degree in one of the following areas: Materials Science and Engineering, Physical Sciences (i.e., Chemistry or Physics) or related fields. Preference will be given to outstanding candidates with experience in running and operating centralized characterization facilities, such as:
Transmission and Scanning Electron Microscopy
Diffraction Techniques
Surface Analysis
The successful candidate will be expected to: formulate and implement a Campus-Wide Materials Characterization Facility, currently used extensively by undergraduate and graduate students in support of research and teaching programs; direct graduate student research; to develop strong programs of sponsored research; and to interact with other members of the faculty.
For full consideration, please submit resume referencing Job #CU-3703R, and the names and addresses of four references by November 30, 1999 to:
Job # CU-3703R
HR Dept., UCI Campus
Berkeley Place Bldg, Suite 1500
Irvine, CA 92697-4600
UCI offers excellent benefits, including a comprehensive insurance package and a minimum three weeks paid vacation. The University of California is an equal opportunity employer committed to excellence through diversity.
The chemical formula for pol(yvinyl butyral) is as follows:
CH / \ --CH2-CH(OH)-CH2-CH(OH)-- + C3H7CHO =} --CH2-CH CH-- poly-(vinyl alcohol) | | O O \ / CH | C3H7
It has been used as a plastic film in laminated glass.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
Dear Lynne, Do you think there is a person in the real world who has demonstrated expertise including, but not limited to, all of those you listed in your job posting? -cy Just feel sorry that even our colleague could be soooooo picky.
} -----Original Message----- } From: Garone, Lynne C [SMTP:GARONEL-at-polaroid.com] } Sent: Tuesday, November 16, 1999 8:33 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Looking for a characterization specialist in silver halide } materi als } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Position available immediately. } } We seek an individual who has demonstrated expertise in a variety of } analytical techniques including, but not limited to, X-ray diffraction, } X-ray fluorescence, AFM, ISS, ESCA, AES, EDS, SSMS, TOFMS, AAS and } electron } microscopy including SEM, FESEM, TEM, AEM, STEM. Specific application of } cryo-ultratome techniques is required. Demonstrated experience with the } application of these techniques, including the development of } three-dimensional maps of the atomic composition of mixed silver halide } emulsion grains is highly desirable. Experience should be demonstrated by } a } patent and/or publication record. } } This person must have a Ph.D. in chemistry, physics or material science, } with at least 5 yrs experience in the photographic industry being highly } desired. The ideal person would also have a deep interest in working in } photographic science, in improving existing film products, and inventing } new } products using state-of- the-art technology. } } The position involves developing technology for product use, with a high } level of interaction with product development teams. } } Resumes can be sent electronically to GaroneL-at-Polaroid.com For more } information, please contact: } Lynne Garone } Polaroid Corp. } 1265 Main St. } Waltham, Ma. 02451 } 781 386-1446 } GaroneL-at-Polaroid.com } }
Anaspec wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi all } On a recent trip to Taiwan a client there showed me a Bausch and Lomb } Nanolab 2100 SEM. He needed some spares for it. Does any one know who I can } contact for assistance on this SEM. } Thanks } } Luc Harmsen } Anaspec, South Africa } Technical support on microscopy. } Tel + 27 (0) 11 476 3455 } Fax + 27 (0) 11 476 7290 } anaspec-at-icon.co.za
Luc,
Contact Derek Saunders at Electron Optic Services, Inc. His e-mail is:
eos-at-magma.ca
He's located in Ontario, Canada.
Ken Converse Quality Images Delta, PA third party SEM service
In a message dated 11/16/99 9:16:29 AM, LFOX1-at-wpo.it.luc.edu writes:
} Can anyone recommend courses that are offered in digital imaging } basics? As we move to a more digital lab, I find that I would like a } firm foundation in knowing why certain choices are preferred for image } capture and manipulation. I am getting bits and pieces through } Photoshop and some of our confocal manuals....but would like to speed } up the learning curve.
We teach a 3 day workshop on digital imaging (processing and analysis) every May (for the past 17 years) at N. C. State University. The course is well attended and well reviewed, and covers all aspects of image processing (removing noise, correcting illumination problems etc.) and measurement (both automatic and manual, including stereological interpretation). The software used is based on Photoshop, so you will have a minimal adjustment in your learning curve. Full info is available at http://members.aol.com/IPCourse/
Advanced Courses of Fluorescence Microscopy and Confocal Microscopy
} From Monday the 16th till Friday the 20th of October 2000, in palazzo Feltrinelli Gargnano (BS), Lake of Garda, Italy. University of Milan- Italy
Courses with an eminently practical approach concerning the use of all light microscopy, including confocal microscopy. For further details, please contact Dr. Annalisa Imberti, Institute of Human Anatomy, University of Milan. Via Mangiagalli 31, 20133 Milan (Italy) or visit our Internet site http://users.unimi.it/~fl2000/
{center} {bold} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Times= New Roman {/param} {bigger} THE UNIVERSITY {italic} of {/italic} LIVERPOOL {/ce= nter}
{center} Materials Science and Engineering {/center}
{center} Department of Engineering {/center}
{center} {smaller} POSTDOCTORAL RESEARCHER IN DEFECT {/center}
{center} MECHANISMS OF PHASE TRANSFORMATIONS {/center}
{center} {underline} {/bold} {bigger} Initial salary within the range =A316,28= 6-=A318,180 {/center}
{flushboth} {/underline} An EPSRC-funded post is available for 3 years, from= January 2000 to study the mechanisms of phase transformations. Prof RC Pond and Dr P Fox have developed models of diffusionless and diffusional phase transformation in terms of the generation, motion and interaction of interfacial dislocations. The object of the research programme will be to apply these models to various transformations in Ti alloys, TiAl intermetallics and TiNi shape- memory alloys, and to seek supporting experimental evidence using TEM. The post would suit materials scientists or physicists with experience of TEM and crystallography. {/flushboth}
{flushboth} Enquiries about the PhD studentship and informal enquiries about the Post Doc position to Prof RC Pond, {/flushboth}
{flushboth} {bigger} Further particulars and details of the application proc= edure may be requested from the Director of Personnel, The University of Liverpool, L69 3BX or 0151 794 2210 (24hr answerphone) or via email: jobs-at-liv.ac.uk {/flushboth}
{flushboth} Web site at http://www.liv.ac.uk {/flushboth}
Hello, I've been trying to find SEM & STM micrographs of this system of PP-Carbon black, for comparison with samples I've synthesized. The system I'm scanning is in powder format, being tried for semiconducting electrical properties. Would highly appreciate it if I could be directed to some suitable link where I could view the images I'm looking for. Even if there's any other similar polymer Carbon powder system, it'll still be a lot of help. Prasad Gopalkrishnan. Macromolecular science & Engg. Case Western Reserve University. Cleveland OH USA
Dear list, a colleague has just asked me a question, that although not totally relevant to the microscopy list, some of list members might be able to enlighten him on the topic, namely :- When does a bacterial cell die - what determines this and what is the typical lifespan in non growing media at room temp? Indeed what defines the point of "death" of the cell - is it merely that it can no longer divide (even though it might continue to absorb nutrients) or is there a another description? The bacteria my colleague is interested in specifically is e-coli but any discussion on this topic might help clarify matters for him. Thanks Giles Sanders -------------------------------------------------------------------------------- -----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre for Analytical SciencesChemistry DepartmentImperial College of Science, Technology and MedicineLondonSW7 2AY----------------------------------------------------------------------------- -----------------------
Yes there is : He is 98 years old and probably RETIRED
----- Original Message ----- } From: Ni, Chao-Ying {CYNi-at-rodel.com} To: 'Garone, Lynne C' {GARONEL-at-polaroid.com} ; {Microscopy-at-sparc5.microscopy.com} Sent: Wednesday, November 17, 1999 4:49 PM
I got several questions regarding to the training CD we are using:
} It is available from International Centre for Diffraction Data, Newtown } Square Corporate Campus, 12 Campus Boulevard, Newtown Square, Pennsylvania } 19073-3273. Voice: 610-325-9810, Fax: 610-325-9823, Email: } information-at-icdd.com. } For the PA regulations, visit Pennsylvania Department of Environmental Protection.htm
} Hasso Weiland } a } Alcoa Technical Center } Alcoa Center, PA 15069 } ACT 221-3133 } * 724 337-3133 } * 724 337-2044 } mailto:hasso.weiland-at-alcoa.com } } usual disclaimer: Alcoa and/or myself have no interests in etc......
we plan to change the electron gun on TEM JEOL 2000FX from freon to SF6 gas el.gun in near future (and of course HT tank too). I would like to ask if anybody has some information about companies which can supply above mentioned gas in Slovak Republic (the producer, contact, www, email etc.) and I will also greatly appreciate some hints or technical advices concerning this installation (and also the quality of gas and prices), because we are the first EM lab in former Czechoslovakia which will do such change. Thank you very much in advance.
With best wishes
Peter Makroczy
makroczy-at-tuke.sk
-- Peter Makroczy Technical University of Kosice Department of Materials Science Park Komenskeho 11 042 00 Kosice Slovak Republic E-mail: makroczy-at-tuke.sk Tel.: +421 95 602 25 40 Fax.: +421 95 633 27 23
I agree with Paul; we use Formvar all the time for immunolabeling=20 ultrathin cryosections. No problem. Besides using protein (e.g., fetal=20 calf serum, BSA, etc.) in all your solutions, you should also microfuge at= =20 high speed your antibodies before use. Small precipitates can occur=20 after storage that can cause bad background everywhere.
Sara Miller
On 17 Nov 1999, Paul Webster wrote:
} Date: 17 Nov 99 11:50:03 -0800 } From: Paul Webster {pwebster-at-mailhouse.hei.org} } To: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} } Cc: MSA listserver submission {Microscopy-at-sparc5.microscopy.com} } Subject: Re: Formvar and Pioloform } =20 } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } =20 } =20 } Reply to: Re: Formvar and Pioloform } Hi Malcolm, } =20 } I use formvar all the time for immunolabeling and have never had a proble= m with it. =20 } Usually, if someone has lots of background over the formvar it can be tra= ced back to being either an antibody problem or crossreactivity of the bloc= king solution with antibody. } =20 } Why complicate things by having to try out different plastics when formva= r works fine? } =20 } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } phone:213 273 8026 } fax: 213 413 6739 } e-mail: pwebster-at-hei.org } http://www.hei.org/htm/aemi.htm } =20 } =20 } Malcolm Haswell wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710=20 Ph: 919 684-3452 FAX: 919 684-8735
There have been inquiries about how to get service on old Sorvall or RMC Ultramicrotomes. These products are all serviced by Ventana Medical Systems, Inc., Tucson, Arizona, USA.
On older systems there are no longer service contracts, but there is on site service or depot service. A one time Preventative Maintenance Call is approximately $900 depending on location.
For exact rates and all other information please contact Technical Customer Care (TCC) at 520-887-2155 or 800-227-2155 and follow the voice prompts for technical support/ hardware support. Visit the web site at: www.Ventanamed.com, look for the Support link.
For any other information please contact me:
Steve Miller North American Sales Manager Microscopy Products Division Ventana Medical Systems, Inc. 3865 N. Business Center Dr. Tucson, AZ 85705 Fax: 520-690-3580 Email: Smiller-at-ventanamed.com
We have used a Pixera camera here for looking at microstructures on our inverted light microscope. For the price you can't beat the Pixera. We are happy with our unit and if a higher resolution camera became available I wouldn't hesitate to buy it. There are better cameras out there but they will run at least $5K and higher. It will depend greatly on the type of resolution and image processing the you will be doing if any. If you would like I could email you an image that we have acquired at 1000X so that you can play around with it. I would definitely suggest taking a sample to some place that has one localy to try out. Pixera will supply you a name and number of a local contact. Good luck and let me know if you need any more input. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif ____________________________________________
Dear John, We purchased a Pixera Pro earlier this year for our Nikon Metallograph. We are very pleased with it. The main advance I can see over the previous version was a big improvement with the software. You now have a full-screen focus mode that makes set-up and focus easy. I consider it excellent value for the money. At 03:20 PM 11/17/99 -0600, you wrote: } } We are seriously considering purchasing a Pixera Pro camera for use on a } Macintosh 8500. It will be used primarily for capturing images from light } microscopes (stereomicrosopes and conventional LM's). We already have a low } light digital camera for fluorescence work so speed of the camera is not } really a concern. } } Does anyone have experience with this camera? Good and bad experiences are } welcome. } } I had heard that they were about to come out with a higher resolution } camera so we waited for over a year -- but nothing has materialized to } date. Now, we really need to purchase a camera in that price range. } } Vendors are welcome to contact me as well. }
} John J. Bozzola, Ph.D., Director
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Try the Particle atlas from McCrone Associates: 800-622-8122 The Atlas is now on CD rom and has a TON of info (LM, SEM, EDS, descriptions, etc.). We've used in on several classes, with delight.
(Caveat: MME has no financial interest in this product.)
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 07:39 AM 11/18/99 -0600, Prasad wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Rick Felten-at-MACDERMID 11/18/99 02:51 PM My high vacuum is not working on my evaporator. I was wondering if 10 militorr is enough vacuum to clean my apertures using a W filament. Ric
I am an electron microscopist with 20 years experience of room temperature fixation and sectioning for TEM. For the past 8 years I have been studying protein trafficking pathways of the intraerythrocytic Plasmodium falciparum (the pathogen that causes the disease Malaria). I am currently in search of used equipment to prepare my samples by way of cryo fixation. I am in need of some type of rapid fixing device (metal to mirror and or immersion), a freeze substitution system (for morphology), and a cryo kit for thin sectioning on either a Leica Ultracut UCT or Reichert Ultracut E (for hydrated immunolabelling).
If any one out there knows of a source of used equipment that meets any of the above needs please contact me, as I need this type of technology to move my research forward.
Many Thanks,
Timothy Schneider, Director of electron Microscopy Room 229 Jeff Hall Thomas Jefferson University 1020 Locust St. Philadelphia, Pa. 19107 215-503-4798 Timothy.Schneider-at-Mail.TJU.EDU
FTIR Specialist University of Minnesota Characterization Facility
The University of Minnesota is seeking an instrument specialist as a staff member in its Materials Characterization Facility. The facility houses an FTIR with microscope capabilities, a surface enhanced Raman spectrometer, and other analytical instruments and microscopes. See our website for details (http://resolution.umn.edu). The person will work mainly in the spectroscopy laboratories. The principle responsibilities of the position include training researchers to operate the spectroscopic instruments, maintaining and operating the FTIR and Raman spectrometers (Nicolet 750, Coherent/SPEX), and assisting users in sample preparation and data interpretation. The position requires a Ph.D. in chemistry, materials science, physics or related discipline. Very strong hands-on experience in spectroscopic techniques and their application to materials characterization is required. Applicants should also have the experience and flexibility to work with other techniques. This is an annually renewable professional appointment; 12 month, 100% time regular appointment with excellent university benefits. Position and salary will be commensurate with education and experience. Please send resume, three letters of recommendation and salary requirements to Stuart McKernan, Characterization Facility, University of Minnesota, 189 Shepherd Labs, 100 Union St. SE, Minneapolis, MN 55455. Screening will begin on December 1, 1999 and end when a suitable applicant is identified. The University of Minnesota is an Equal Opportunity Educator and Employer.
__________________ Stuart McKernan stuartm-at-tc.umn.edu Director Office:(612) 626-7594 IT Characterization Facility, University of Minnesota Desk:(612) 624-6009 100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368
} Questions have been raised about my comment on the formula for polyvinyl } butyral. It would probably have been more accurate and easier to } understand if I had stated and written the chemical equation as shown below. } } } } The chemical reaction for producing polyvinyl butyral is as follows: } } CH CH } / \ / \ } --[CH2-CH-CH2-CH]-- + C3H7CHO =} --[CH2-CH CH-CH2-CH CH]--} } | | butyraldehyde | | | | } OH OH O O O O } poly-(vinyl \ / \ / } alcohol CH CH } | | } C3H7 C3H7 } polyvinyl butyral } } That is, polyvinyl butyral is produced by reacting butyraldehyde (which is } the common four-carbon aldehyde: } CH3-CH2-CH2-CHO ) } with poly-(vinyl alcohol) (which is a linear polymer essentially } consisting of a string of alternating CH2 and CH(OH) groups, as shown } above). } } In the above equation I have used double dashes (--) outside square } brackets --[]-- to indicate that the structure inside the square brackets } is repeated many times to form the large linear molecules of the high } molecular weight polymers that we know as polyvinyl alcohol and polyvinyl } butyral. } } I hope this helps clear things up. The reaction I gave for formvar (which } is produced by reacting polyvinyl alcohol with formaldehyde HCHO) could be } similarily modified for clarity. }
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Rick Felten-at-MACDERMID } 11/18/99 02:51 PM } My high vacuum is not working on my evaporator. I was wondering if 10 } militorr is enough vacuum to clean my apertures using a W } filament. } Ric
rfelten-at-Macdermid.com-at-Sparc5.Microscopy.Com wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Rick Felten-at-MACDERMID } 11/18/99 02:51 PM } My high vacuum is not working on my evaporator. I was wondering if 10 } militorr is enough vacuum to clean my apertures using a W } filament. } Ric
Ric
You might try 1 micron diamond paste and a cut-knap polishing cloth or even just a lint-free cloth. Put some paste on the cloth, put the aperture on the paste, put your finger on the aperture and rub it around. No damage from overheating, no reforming of the crystal structure and no vacuum needed. Clean ultrsonically using your preferred solvents (I like water and Joy or Micro). The only way I have ever ruined an aperture this way is by folding over a .001" thick foil upon occassion.
I've also recovered apertures that had suffered damage in vacuum evaporators. I won't even consider any other way of cleaning any more.
Hi CY & list buddies, If 2 people exist on our planet with this background & they may, this list server is a good place to find them. I can do half of the tricks asked & understand the fundamentals of most others. It stands to reason the others can do more, much more. What they are really looking for is a skilled, highly motivated analysts in the film business & there are a # of competitors in the business to recruit from. I suggest that this person does exist. The add may describe the person that is leaving that position or a person known to the solicitor & whom they want to hire. The position may only exist because they have knowledge of someone with this skill set & perhaps something more that we don't know of. Also consider that the experience required may be that of interpreting the data rather than personally mastering all machines. It is not uncommon to run a add to satisfy equal opportunity recruiting requirements but the person hiring knows just who they want to hire & they have their listed their qualifications. Naturally no one will own up to this but I don't think is an uncommon tactic. EOE legislation has created real problems in filling nitch positions in science. In case you don't know, the penalty for hiring the wrong person can be severe. Is it legal, right, etc... that's a matter of philosophy. Is it reality, yes I believe it is. Consider that some plain vanilla advertisement was run that caused you to take off work, & cross the county pursue a position while all the time it was a done deal waiting on formalism. Wouldn't be very fair to you would it? At 1st I too thought this was an abuse of the list server but after thinking about it & realizing that similar (highly specific) adds show up in news papers. Posting to this list server is a good faith effort at finding anyone that can fill the bill. Correct me if I am wrong but I seem to recall that we have 3000ish subscribers. This brings the add back to supporting EOE policies. If this position is not already in the bag & the person does not exist, then a person with most of the requested skill set & the confidence to present themselves probably has the character they want.
just today's opinion, Bruce Brinson Rice U.
Ni, Chao-Ying wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Lynne, } Do you think there is a person in the real world who has } demonstrated expertise including, but not limited to, all of those you } listed in your job posting? } -cy } Just feel sorry that even our colleague could be soooooo picky. } } } -----Original Message----- } } From: Garone, Lynne C [SMTP:GARONEL-at-polaroid.com] } } Sent: Tuesday, November 16, 1999 8:33 PM } } To: Microscopy-at-Sparc5.Microscopy.Com } } Subject: Looking for a characterization specialist in silver halide } } materi als } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Position available immediately. } } } } We seek an individual who has demonstrated expertise in a variety of } } analytical techniques including, but not limited to, X-ray diffraction, } } X-ray fluorescence, AFM, ISS, ESCA, AES, EDS, SSMS, TOFMS, AAS and } } electron } } microscopy including SEM, FESEM, TEM, AEM, STEM. Specific application of } } cryo-ultratome techniques is required. Demonstrated experience with the } } application of these techniques, including the development of } } three-dimensional maps of the atomic composition of mixed silver halide } } emulsion grains is highly desirable. Experience should be demonstrated by } } a } } patent and/or publication record. } } } } This person must have a Ph.D. in chemistry, physics or material science, } } with at least 5 yrs experience in the photographic industry being highly } } desired. The ideal person would also have a deep interest in working in } } photographic science, in improving existing film products, and inventing } } new } } products using state-of- the-art technology. } } } } The position involves developing technology for product use, with a high } } level of interaction with product development teams. } } } } Resumes can be sent electronically to GaroneL-at-Polaroid.com For more } } information, please contact: } } Lynne Garone } } Polaroid Corp. } } 1265 Main St. } } Waltham, Ma. 02451 } } 781 386-1446 } } GaroneL-at-Polaroid.com } } } }
Hello all..... I'm looking for a procedure to do a quantification of abidin-biotin inmuno= labeling ( sorry, I'm not biologist )...using a PC and a Image analisis Program...=
Cannot be sure about that. There may well be too much residual oxygen and this would form a moly-oxide. You can reduce the residual active gases to near "nothing" by purging the system with Argon or Nitrogen and pumping it out for a second time. If you don't have a needle valve inlet, you could purge by placing a small plastic beaker with liquid nitrogen into the belljar. Be quick with that beaker or you get too much condensation. Too much liq N2 in the beaker would, after fierce boiling, turn into N2 slush. This is undesirable because the slush is slow to sublime. Try first with one bad aperture. I think it will work. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Friday, November 19, 1999 5:51 AM, "rfelten-at-Macdermid.com"-at-sparc5.microscopy.com [SMTP:"rfelten-at-Macdermid.com" -at-sparc5.microscopy.com] wrote: } } } } Rick Felten-at-MACDERMID } 11/18/99 02:51 PM } My high vacuum is not working on my evaporator. I was wondering if 10 } militorr is enough vacuum to clean my apertures using a W } filament. } Ric } }
} } No. I tried to clean Mo apertures under 10-3 pressure. The } oxidation ruined the apertures.
} Rick Felten-at-MACDERMID } 11/18/99 02:51 PM } My high vacuum is not working on my evaporator. I was wondering if 10 } militorr is enough vacuum to clean my apertures using a W filament.
One possibility would be to purge your evaporartor with an inert gas .. e.g., argon, but even N2 would work.
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I know a lot of vendors read this list so hopefully, I will get some information. I have been asked to get an idea of the cost of a metallurgical microscope which will also do transmitted light, 100 to 1000x in air. Quotes can be faxed to 617/638-5591 or sent to me at Biomaterials, 801 Albany St., rm. 210, Boston, MA 02110, Attention, Ron
I had similiar problems, but my version of the eXL is so old that not much could be done. I could not find a replacement monitor for less than ~ $2.5 K here in the USA, which could match the specs. In the end I bought an upgrade package from Oxford/Link which for ~ $1.6K (about 2 years ago) that allowed me to use a standard VGA Monitors which I have around the lab.
It involves swapping out a couple of chips a new mother board and some new software drivers for the eXL .
When you have acquired several images of the same region with different focu= s=20 settings, it is attractive to combine them into an =93extended focus composi= te=94=20 by keeping the pixels from each of the several images that have the best=20 focus. Generally, it is agreed that the =93best focus=94 is characterized by= the=20 greatest local contrast and the maximum high-frequency components in the=20 image (these are the algorithms used in most auto-focus procedures). After=20 evaluating several different tools to measure =93goodness of focus=94 on a=20 pixel-by-pixel basis in two or more images, I've have selected the local=20 variance, calculated in a 5 pixel wide circular neighborhood, as a useful=20 criterion. If anyone out there has performed similar evaluations and believe= s=20 there is another worthy candidate for selecting the best focus pixel, I=92d=20 sure like to hear about it.
If anyone wants to see typical results using the variance, an example is=20 on-line at http://members.aol.com/IPTK/focus.htm We=92ve programmed this as a Photoshop-compatible plug-in that you can downl= oad=20 from the site. It will run in a wide variety of programs including NIH-Image= ,=20 Image Pro Plus, Canvas, Digital Darkroom, etc., on both Mac and Windows=20 platforms). The plug-in requires the Image Processing Tool Kit, which many o= f=20 the readers of this newsgroup already have.
Hello out there, Any experts in staining cell coats, glycocalyx or mucin using alcian blue or other components, please foward techniques for standard TEM to the list, or us. From old protocols we have 1 - 0.1% alcian blue with 3% GA. Thanks
rfelten-at-Macdermid.com-at-Sparc5.Microscopy.Com wrote: } } ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.} } Rick Felten-at-MACDERMID } 11/18/99 02:51 PM } My high vacuum is not working on my evaporator. I was wondering if 10 } militorr is enough vacuum to clean my apertures using a W } filament. } Ric
Ric,
We produce and clean thousands of apertures each year and we do not believe you can clean a moly aperture at 10 militorr due to oxydation.
Perhaps you can clean larger holes with a polish, but we are now producing apertures as small as 1 micron in Pt and Moly and would be very concerned that the wall could easily be damaged in our ultra-small sized holes.
Would it be possible to switch to a platinum aperture rather than Moly? I believe we are the largest aperture producer in the world and 75 percent of our discs and strips are done in Platinum, and you would avoid the vacuum issue that way.
Please contact me if you wish to discuss this further,
John Arnott -- LADD RESEARCH 131 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
I think that if it is a "done deal" or if the position is "wired in," then someone should not post a position on the Listserver. This isn't a sanctioned forum for advertising positions to satisfy legal requirements for hiring people. It is an excellent means of finding a qualified individual to satisfy their staffing needs. Let people who have "done deals" do it in the paper -not here. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Bruce Brinson [mailto:brinson-at-rice.edu] } Sent: Thursday, November 18, 1999 11:36 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Re: Looking for a characterization specialist in } silver halide } ma teri als } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Hi CY & list buddies, } If 2 people exist on our planet with this background & } they may, this list } server is a good place to find them. I can do half of the } tricks asked & } understand the fundamentals of most others. It stands to } reason the others can } do more, much more. What they are really looking for is a } skilled, highly } motivated analysts in the film business & there are a # of } competitors in the } business to recruit from. } I suggest that this person does exist. The add may } describe the person that } is leaving that position or a person known to the solicitor & } whom they want to } hire. The position may only exist because they have knowledge } of someone with } this skill set & perhaps something more that we don't know } of. Also consider } that the experience required may be that of interpreting the } data rather than } personally mastering all machines. } It is not uncommon to run a add to satisfy equal } opportunity recruiting } requirements but the person hiring knows just who they want } to hire & they have } their listed their qualifications. Naturally no one will own } up to this but I } don't think is an uncommon tactic. EOE legislation has } created real problems in } filling nitch positions in science. In case you don't know, } the penalty for } hiring the wrong person can be severe. Is it legal, right, } etc... that's a } matter of philosophy. Is it reality, yes I believe it is. } Consider that some plain vanilla advertisement was run } that caused you to } take off work, & cross the county pursue a position while all } the time it was a } done deal waiting on formalism. Wouldn't be very fair to you would it? } At 1st I too thought this was an abuse of the list server } but after thinking } about it & realizing that similar (highly specific) adds show } up in news } papers. Posting to this list server is a good faith effort } at finding anyone } that can fill the bill. Correct me if I am wrong but I seem } to recall that we } have 3000ish subscribers. This brings the add back to } supporting EOE policies. } If this position is not already in the bag & the person } does not exist, then } a person with most of the requested skill set & the } confidence to present } themselves probably has the character they want. } } just today's opinion, } Bruce Brinson } Rice U. } } } } Ni, Chao-Ying wrote: } } } } -------------------------------------------------------------- } ---------- } } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------- } ---------. } } } } Dear Lynne, } } Do you think there is a person in the real } world who has } } demonstrated expertise including, but not limited to, all } of those you } } listed in your job posting? } } -cy } } Just feel sorry that even our colleague could be } soooooo picky. } } } } } -----Original Message----- } } } From: Garone, Lynne C [SMTP:GARONEL-at-polaroid.com] } } } Sent: Tuesday, November 16, 1999 8:33 PM } } } To: Microscopy-at-Sparc5.Microscopy.Com } } } Subject: Looking for a characterization specialist } in silver halide } } } materi als } } } } } } } -------------------------------------------------------------- } ---------- } } } The Microscopy ListServer -- Sponsor: The Microscopy } Society of America } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } } } -------------------------------------------------------------- } ---------. } } } } } } } } } Position available immediately. } } } } } } We seek an individual who has demonstrated expertise in a } variety of } } } analytical techniques including, but not limited to, } X-ray diffraction, } } } X-ray fluorescence, AFM, ISS, ESCA, AES, EDS, SSMS, TOFMS, AAS and } } } electron } } } microscopy including SEM, FESEM, TEM, AEM, STEM. } Specific application of } } } cryo-ultratome techniques is required. Demonstrated } experience with the } } } application of these techniques, including the development of } } } three-dimensional maps of the atomic composition of mixed } silver halide } } } emulsion grains is highly desirable. Experience should } be demonstrated by } } } a } } } patent and/or publication record. } } } } } } This person must have a Ph.D. in chemistry, physics or } material science, } } } with at least 5 yrs experience in the photographic } industry being highly } } } desired. The ideal person would also have a deep } interest in working in } } } photographic science, in improving existing film } products, and inventing } } } new } } } products using state-of- the-art technology. } } } } } } The position involves developing technology for product } use, with a high } } } level of interaction with product development teams. } } } } } } Resumes can be sent electronically to } GaroneL-at-Polaroid.com For more } } } information, please contact: } } } Lynne Garone } } } Polaroid Corp. } } } 1265 Main St. } } } Waltham, Ma. 02451 } } } 781 386-1446 } } } GaroneL-at-Polaroid.com } } } } } } } }
Bravo, Bruce You express exactly what I was thinking about such "advertisements".
} Date: Thu, 18 Nov 1999 22:35:49 -0600 } From: Bruce Brinson {brinson-at-rice.edu} } Subject: Re: Looking for a characterization specialist in silver halide ma teri } als } To: Microscopy-at-sparc5.microscopy.com } Reply-to: brinson-at-rice.edu } Organization: Rice University } X-Mailer: Mozilla 4.06 [en] (Win95; U) } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev Ph. D. UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
-----Original Message----- } From: Walck. Scott D. {walck-at-ppg.com} To: 'brinson-at-rice.edu' {brinson-at-rice.edu} ; 'Microscopy' {microscopy-at-Sparc5.Microscopy.Com} } } I think that if it is a "done deal" or if the position is "wired in," then } someone should not post a position on the Listserver. This isn't a } sanctioned forum for advertising positions to satisfy legal requirements for } hiring people. It is an excellent means of finding a qualified individual } to satisfy their staffing needs. Let people who have "done deals" do it in } the paper -not here. } -Scott
The person posting may not know it there is a ringer in the game. Also I seldom see a job filled by an applicant that met all the qualifications. You ask for what you want and take what you can get.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00
Sometimes an institution must list such detailed/individualized qualifications because hiring policy precludes listing the social security number of the preferred applicant as a necessary qualificaton.
Paul Grover Chief Microscopist and Bottle Washer Microvista Laboratory
----Oorspronkelijk bericht----- Van: "laboratorio-at-abasto.dataco27.com.ar"-at-sparc5.microscopy.com {"laboratorio-at-abasto.dataco27.com.ar"-at-sparc5.microscopy.com} Aan: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com} Datum: vendredi 19 novembre 1999 9:02 Onderwerp: hematoxilin stein?
} ----------------------------------------------------------- ------------- } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} Question: =85How can I do an hematoxilin eosin stein? }
Eh, that's a realy very basic question...
This is from an online manual called "UNIVERSITY OF BRISTOL - Histological Staining Techniques". I've lost the URL but you should be able to find the file trough Altavista or another search-engine...
Haematoxylin & eosin (H&E)
1. Dewax sections, rinse in alcohol, rinse in water. 2. Harris' haematoxylin - 10 minutes. 3. Wash and blue in running tap water - 1 minute. 4. Differentiate in acid alcohol (1% HCl in 70% alcohol) - 10 seconds. 5. Wash and blue in running tap water - 5 minutes. 6. Eosin - 4 minutes. 7. Wash in tap water. 8. Dehydrate, clear and mount. Results: Nuclei - blue. Other tissue components - shades of red and pink. Harris' haematoxylin Haematoxylin - 5g. 100% alcohol - 50mls Potassium alum - 100g. Distilled water - 1 litre Mercuric oxide - 2.5g. Glacial acetic acid - 40mls Dissolve the potassium alum in the water by warming and stirring. Dissolve the haematoxylin in the alcohol and add. Bring rapidly to the boil remove from heat and add the mercuric oxide. Cool, add the acetic acid and filter. Ready for use immediately.
Eosin 1% eosin Y (yellowish) in TAP water. Add a crystal of thymol to prevent the growth of moulds.
Perhaps it would be better to look for a good book on the basics of histological/microscopical technique...
* Step 1: most other hematoxylin solutions can be used instead of Harris': Mayer's, Delafield's, Cole's, Ehrlich's... I usualy use Mayer's mod. Lillie.
The use of mercuric oxide as an oxidant in the solution poses serious problems. It can be substituded trough sodium iodate (100mg/g hematoxylin to give a solution with partially oxidized hematoxylin (=3D hematein)), or use another hematoxylin formulation.
* Step 6: Instead of the eosin solution I use a mixture of 1g eosin "Y" and 1g orange G in 100ml Aq. Dest, giving far better differentiation in the reds.
The 38th Annual Conference of the Microscopy Society of Southern Africa takes place in Bloemfontein, South Africa, next week (30 November to 3 December)
The Table of Contents (authors, titles and page numbers) of the Proceedings (Volume 29, 1999) can be viewed at the following web address:
http://www.ru.ac.za/emu/mssa99.htm
For more information about the society and MSSA '99 go to the MSSA web site:
http://www.uct.ac.za/depts/emu/mssa/index.htm
or
http://www.uovs.ac.za/nat/mssa99/conference.htm
Robin H Cross Vice-President : Microscopy Society of Southern Africa EM Unit, Rhodes University, PO Box 94, Grahamstown, South Africa tel: +27 46 603 8168 - fax: +27 46 622 4377 email: R.Cross-at-ru.ac.za
*** remember that ICEM-15 takes place in Durban, South Africa in 2002 ***
In the same vein - My business partner & I used to subscribe to the Commerce Business Daily, in which were advertised numerous Requests for Proposals. Ha. We responded to a number of them, but never even received back any more than an acknowledgement of receipt. No reviews; no feedback; nothing at all for our efforts. Then we tried preparing a Small Business Innovative Research proposal; got our first response: "Already doing that." So we have written off the entire business of research for The Government as a waste of our time. Probably a waste of our Taxpayers' money, too, if "done deals" are what were advertised there. And we had to pay good money for the Commerce Business Daily.
Best regards, George Langford, Sc.D. amenex-at-amenex.com http://www.amenex.com/
The tilt angle you can use depends a lot on the sample. For an automated measurement or the reconstruction of the surface, the computer has to locate the identical position on both images. If the tilt angle is too large and the images too different, then no calculation is possible. For example, a tilt of 10 degrees may hide certain areas on one image that are visible on the other, if the sample has prominent structures that rotate into view. Of course for those areas no calculation is possible. On the other hand a larger tilt angle may be possible, for example for shallow indentations.
The tilt angle of 6-10 degrees is usually used as it enables the brain to interpret the images in 3 dimensions when looked at with appropriate glasses (green-red or polarized or similar). For higher angles the 3D effect disappears because the brain can't interpret it anymore. All these numbers are approximate, of course.
I am not sure, what you have done with Excel, but an automated system allows you to reconstruct the entire surface in a few seconds, plot it in 3-D, rotate it, texture it, etc. A better Z-resolution is also possible by using the local neighborhood to find position pairs on the two images. Also alignment of the images and 3-D measurements should be easier.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: Dusevich, Vladimir[SMTP:DUSEVICHV-at-UMKC.EDU] } Sent: Tuesday, November 16, 1999 10:35:15 AM } To: 'Microscopy-at-MSA.Microscopy.Com' } Subject: RE: software for height measurement from stereo pairs?? } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I routinely use tilt angles of 15-30 degrees. It is too high value for visual observation of a stereopair, but often it is the best choice angle in terms of accuracy of stereo measurements (as long as I do not have overlapping, I can increase angle). Since I perform stereo measurements not often, I did not by any special software. Microsoft Excel works just fine for me.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 3127 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: Michael Bode [mailto:mb-at-soft-imaging.com] } Sent: Monday, November 15, 1999 11:05 AM } To: 'Microscopy-at-MSA.Microscopy.Com' } Subject: FW: software for height measurement from stereo pairs?? } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Yes, we do quantitative evaluation of stereo pairs. } } There are a number of factors that impact the height } resolution one can } get from those images. In essence there is a trigonometric } function that } relates the z-resolution to the x/y resolution. The numerical factor } depends on a number of other parameters with the stereo angle } being the } most important one. For typical stereo angles of 6 - 10 degrees, the } factor is of the order of .1 - .2. In other words, the height } resolution } is about 1/5th to 1/10th of the x/y resolution (for example: x/y } resolution 1 micron/pixel -} height resolution about 5 to 10 microns). } It is possible to improve this through sub-pixel operations. } } Please contact me off-line if you need more information. } } Michael } } Michael Bode, Ph.D. } Soft Imaging System Corp. } 1675 Carr St., #105N } Lakewood, CO 80215 } =================================== } phone: (888) FIND SIS } (303) 234-9270 } fax: (303) 234-9271 } email: mailto:info-at-soft-imaging.com } web: http://www.soft-imaging.com } =================================== } } } } -----Original Message----- } } From: Dr. Gary Gaugler [mailto:gary-at-gaugler.com] } Sent: Saturday, November 13, 1999 9:35 AM } To: Brendan Griffin } Cc: Michael Bode } Subject: Re: software for height measurement from stereo pairs?? } } } Try Soft-Imaging's analySIS. It has optional plug-in modules that } I believe can do this. } } gary g. } } } At 09:12 PM 11/12/99 , you wrote: } } ------------------------------------------------------------- } ---------- } - } } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------- } ---------- }
Linda, i have no idea where you are located, but I am taking a course in this very subject at the University of Texas in Arligton. we are using a textbook called The Image Processing Handbook by John Russ. the book can be difficult to follow, but it is in print, and so should be available from anywhere. You will probably have to order it special,though. If you are anywhere near North Texas, I would recommend that you contact Dr. Arnott at UTA and see when he will offer this class agian. I'm having a ball in it! (Are you listening Dr. Arnott? Can I have 10 extra points?) Feel free to contact me if I can answer any questions Wanda Shotsberger Harris Methodist Hospital Fort Worth Texas ---------- } From: Linda Fox To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Can anyone recommend courses that are offered in digital imaging basics? As we move to a more digital lab, I find that I would like a firm foundation in knowing why certain choices are preferred for image capture and manipulation. I am getting bits and pieces through Photoshop and some of our confocal manuals....but would like to speed up the learning curve. Thanks, Linda Fox lfox1-at-wpo.it.luc.edu
Can someone please help me determine, or direct me to a comprehensive guide for determining, the appropriate gloves for handling each of the following chemicals?
I have talked with our environmental health and safety officer and read the permeation/degradation, resistance guides in our chemical and industrial safety vendor catalogs. Some of our chemicals are not on any company's glove guide but several of the ones that are require using a bulky heavy duty type glove made from Viton, Butyl, Neoprene or Norfoil and best suited for gross chemical handling. Is there a U.S. distributor for form fitting flexible gloves made from these or equivalent materials?
Thank you.
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
The John Russ books can be easily purchased from Amazon.com.
rob
Robert S. Geske Research Associate Center for Comparative Medicine and Department of Pediatrics Baylor College of Medicine
-----Original Message----- } From: Shotsberger-Gray, Wanda [SMTP:WandaShotsberger-Gray-at-hmhs.com] Sent: Monday, November 22, 1999 10:13 AM To: Linda Fox; Microscopy-at-sparc5.microscopy.com
Linda, i have no idea where you are located, but I am taking a course in this very subject at the University of Texas in Arligton. we are using a textbook called The Image Processing Handbook by John Russ. the book can be difficult to follow, but it is in print, and so should be available from anywhere. You will probably have to order it special,though. If you are anywhere near North Texas, I would recommend that you contact Dr. Arnott at UTA and see when he will offer this class agian. I'm having a ball in it! (Are you listening Dr. Arnott? Can I have 10 extra points?) Feel free to contact me if I can answer any questions Wanda Shotsberger Harris Methodist Hospital Fort Worth Texas ---------- } From: Linda Fox To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Can anyone recommend courses that are offered in digital imaging basics? As we move to a more digital lab, I find that I would like a firm foundation in knowing why certain choices are preferred for image capture and manipulation. I am getting bits and pieces through Photoshop and some of our confocal manuals....but would like to speed up the learning curve. Thanks, Linda Fox lfox1-at-wpo.it.luc.edu
Our current Fisher catalog has a guide for gloves on pp. 805-807. Cole-Parmer has a shorter one on page 943. These are not comprehensive guides, but we have found them useful.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 tindallr-at-missouri.edu http://www.biotech.missouri.edu/emc/
-----Original Message----- } From: JIM ROMANOW [mailto:bsgphy3-at-uconnvm.uconn.edu] Sent: Monday, November 22, 1999 12:15 PM To: microscopy-at-Sparc5.Microscopy.Com
Can someone please help me determine, or direct me to a comprehensive guide for determining, the appropriate gloves for handling each of the following chemicals?
I have talked with our environmental health and safety officer and read the permeation/degradation, resistance guides in our chemical and industrial safety vendor catalogs. Some of our chemicals are not on any company's glove guide but several of the ones that are require using a bulky heavy duty type glove made from Viton, Butyl, Neoprene or Norfoil and best suited for gross chemical handling. Is there a U.S. distributor for form fitting flexible gloves made from these or equivalent materials?
Thank you.
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-2131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
There is an opening for a post-doctoral position in my laboratory for electron-crystallographic studies on membrane protein channels. The following advertisement is being posted in the Journal Science. The electron microscope facility at Scripps is extremely well equipped. We have a shared e.m. facility which has a Philips CM200FEGT, a CM120, a CM100 and 2 EM208, one Perkin Elmer PDS microdensitometer, one Zeiss scanner and an upright optical diffractometer. The cryo work is done on the CM200 and CM120. The image processing in my laboratory is carried out on a DEC-alpha machine and a SGI O2. These are connected to a cluster of several accessible UNIX workstations in the Department. The departmental e.m. suite include facilities for carrying out freeze-fracture and rotary shadowing of samples.
POSTDOCTORAL POSITION is available immediately for structural studies of membrane proteins (water channels, ABC transporters, protein toxins) using electron microscopy and image processing. Candidates with a background in structural biochemistry, biophysics or protein expression should send a curriculum vitae and names of three references to:
Dr. Alok K. Mitra Assistant Professor Department of Cell Biology The Scripps Research Institute 10550 N. Torrey Pines Road La Jolla, CA 92037. E-mail: mitra-at-scripps.edu.
Selected Publications:
1. Mitra, A.K., Yeager, M., van Hoek, A. N., Wiener, M. C.and Verkman, A. S. (1994). Projection Structure of the CHIP28 Water Channel in Lipid Bilayer Membranes at 12-=C5 Resolution. Biochemistry 33, 12735-12740. 2. Mitra, A. K., van Hoek, A. N., Wiener, M. C., Verkman, A. S. and Yeager, M. (1995). The CHIP28 water channel visualized in ice by electron crystallography. Nature Structure Biology. 2, 726-72926. 3. Verkman, A. S., van Hoek, A. N., Ma, T., Frigeri, A., Skach, W. R., Mitra, A. K., Tamarappoo B. K. and Farinas J. (1996) Water transport across mammalian cell membranes. Am. J. Physiol.. 270, C12-C30. 4. Cheng, A., van Hoek, A. N., Yeager, M., Verkman, A. S. and Mitra, A. K. (1997) Structural Organization in a Human Water Channel. Nature. 387, 627-630. 5. Yeager, M., Unger, V. M. and Mitra, A. K. (1999) Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysis. Method. Enzym. 294, 135-180. 6. Verkman, A. S. and Mitra, A. K. Structure and function of aquaporin water channels - invited review. Am. J. Physiol. 276: in Press.
} Hello offended Microscopists ! } } In the same vein - My business partner & I used to subscribe to the } Commerce Business Daily, in which were advertised numerous Requests } for Proposals. Ha. We responded to a number of them, but never even } received back any more than an acknowledgement of receipt. No reviews; } no feedback; nothing at all for our efforts. Then we tried preparing } a Small Business Innovative Research proposal; got our first response: } "Already doing that." So we have written off the entire business of } research for The Government as a waste of our time. Probably a waste } of our Taxpayers' money, too, if "done deals" are what were advertised } there. And we had to pay good money for the Commerce Business Daily. } } Best regards, } George Langford, Sc.D. } amenex-at-amenex.com } http://www.amenex.com/
I think that you do not understand how the system works. First off, the system requires 30 days notice in the CBD of any impending actions. If it is an announcement of an imminent action, unless you have some Earth-shaking reason to denounce that action, it is indeed a done deal.
But there are announcements that are inquisitive in nature and if your response is judged lesser than other responses, you will indeed get a Dear John letter.
SBIRs and other announcements are an area that I think you grossly misunderstand. The CBD is essentially a forum for legally announcing what will take place, barring some substantial justification to the contrary. The point is that someone or some company has done their homework and initiated a project or proposal to a government agency or activity that was accepted as presented. The CBD then reflects the acceptance of that innovative act. If you expect the CBD or SBIR to be a source for parasitic action, you are dead wrong. The innovation and initiative is done long before it reaches the CBD. If you do not innovate and create and seek sources for your products and services, you are not likely to have a good day. "You have to kiss a lot of frogs to find a prince."
I would encourage you to better understand how the system works before you criticize it. Remember, this system is the product of "our government," and as such, it is supposed to equalize dealings of the dispensation of taxpayer funds.
In response to my Sour Grapes about Guv'mint Research:
.. heavy snippage ...
} ... Commerce Business Daily, in which were advertised numerous } Requests for Proposals. Ha. ... Then we tried preparing a Small } Business Innovative Research proposal; got our first response: } "Already doing that." So we have written off ...
Gary Gaugler says:
} I think that you do not understand how the system ...
Shouldn't "The System" be capitalized ?
} ... works. First off, the system requires 30 days notice in the } CBD of any impending actions. If it is an announcement of an } imminent action, unless you have some Earth-shaking reason } to denounce that action, it is indeed a done deal.
The announcements were "Requests for Proposals." With deadlines & addresses to whom the proposals were to be sent. No "Done Deals" implied, stated, or writ 'twixt the lines. Heck, they cheerfully provided exact details about the formats of said proposals.
} But there are announcements that are inquisitive in nature and } if your response is judged lesser than other responses, you will } indeed get a Dear John letter.
Yup. Constructive as all get out.
} SBIRs and other announcements are an area that I think you grossly } misunderstand.
We even attended the SBIR briefings - more than once. "Small" is a concept that I admit is hard to comprehend in the Real World. "Small" to SBIR is a company with less than $250M in sales (or is it $25M ?). Either way is "huge" in my world, where we sink or swim on our own wits & quality of service, and we'll take another 100 years to reach even $25M in total sales for our entire history.
} The CBD is essentially a forum for legally announcing what will } take place, barring some substantial justification to the contrary. } The point is that someone or some company has done their homework } and initiated a project or proposal to a government agency or } activity that was accepted as presented. The CBD then reflects } the acceptance of that innovative act.
With a request for more proposals ? Why would anyone in his right mind contribute an utterly wasted hundred man-hours or so if he understood that it was a mere formality to justify an agency's decision to issue a contract without competitive bidding ? That's not what those announcements said. They solicited proposals that the reader was led to expect would receive fair consideration.
} If you expect the CBD or SBIR to be a source for parasitic action,
Sounds insulting. We were trying to compete on what we thought was a level playing field, with our own ideas in response to requests for ideas; and we backed 'em up with well thought out and documented budgets, schedules of what was to be done, when, and so on.
} ... you are dead wrong. The innovation and initiative is done long } before it reaches the CBD. If you do not innovate and create and } seek sources for your products and services, you are not likely to } have a good day.
My suspicion is that the RFP's were fishing for free ideas.
} "You have to kiss a lot of frogs to find a prince."
No thanks; there are no Princes in that pond.
} I would encourage you to better understand how the system works } before you criticize it.
Thought I did. Had two long-term, innovative research projects which I thought I had won fair & square when I taught at Drexel University.
} Remember, this system is the product of "our government," and } as such, it is supposed to equalize dealings of the dispensation } of taxpayer funds.
That was what I was sour graping about. Didn't seem fair to me - all one way, with no feedback. And I haven't begun to sound off about the way the NSF treated research-fund seekers in the universities. Too many proposals chasing too little funding, with the "riches" going to the most persistent (read: repetitive) proposal writers, who would then use that funding to support yet more proposal writing. A gigantic Ponzi scheme, but in reverse. Forgive me if it's all been changed in the last eighteen years since I participated in that circus.
Best regards, George Langford, Sc.D., in SE PA, happily doing consulting for folks who actually want ideas & answers for their problems and who pay in real dollars (no overhead fudge factors) for the work we actually do for them and for what we said we'd charge. amenex-at-amenex.com http://www.amenex.com/
At 05:25 PM 11/22/99 , you wrote: } Hi Gary & Microscopists ! } } In response to my Sour Grapes about Guv'mint Research: } } ... heavy snippage ... } } } ... Commerce Business Daily, in which were advertised numerous } } Requests for Proposals. Ha. ... Then we tried preparing a Small } } Business Innovative Research proposal; got our first response: } } "Already doing that." So we have written off ... } } Gary Gaugler says: } } } I think that you do not understand how the system ... } } Shouldn't "The System" be capitalized ?
It is...by you and I.
} } ... works. First off, the system requires 30 days notice in the } } CBD of any impending actions. If it is an announcement of an } } imminent action, unless you have some Earth-shaking reason } } to denounce that action, it is indeed a done deal. } } The announcements were "Requests for Proposals." With deadlines } & addresses to whom the proposals were to be sent. No "Done Deals" } implied, stated, or writ 'twixt the lines. Heck, they cheerfully } provided exact details about the formats of said proposals.
Well...you do not get the picture.
} } } But there are announcements that are inquisitive in nature and } } if your response is judged lesser than other responses, you will } } indeed get a Dear John letter. } } Yup. Constructive as all get out.
Of course.
} } } SBIRs and other announcements are an area that I think you grossly } } misunderstand. } } We even attended the SBIR briefings - more than once. "Small" } is a concept that I admit is hard to comprehend in the Real World. } "Small" to SBIR is a company with less than $250M in sales (or is } it $25M ?). Either way is "huge" in my world, where we sink or } swim on our own wits & quality of service, and we'll take another } 100 years to reach even $25M in total sales for our entire history.
SBIR is done is three phases. Phase I is the investigative phase and is usually less than $90K ($100K is the legal limit but no one does this that I know of). Phase II is up to $750K for proof of concept. Phase III is open since it is not funded by the government. By Phase II, it is assumed that the idea/project is a good one and will garner industrial or venture capital to continue.
} } The CBD is essentially a forum for legally announcing what will } } take place, barring some substantial justification to the contrary. } } The point is that someone or some company has done their homework } } and initiated a project or proposal to a government agency or } } activity that was accepted as presented. The CBD then reflects } } the acceptance of that innovative act. } } With a request for more proposals ? Why would anyone in his right } mind contribute an utterly wasted hundred man-hours or so if he } understood that it was a mere formality to justify an agency's } decision to issue a contract without competitive bidding ? That's } not what those announcements said. They solicited proposals that } the reader was led to expect would receive fair consideration.
You were reactive rather than proactive. You got what you earned.
} } If you expect the CBD or SBIR to be a source for parasitic action, } } Sounds insulting. We were trying to compete on what we thought was } a level playing field, with our own ideas in response to requests } for ideas; and we backed 'em up with well thought out and documented } budgets, schedules of what was to be done, when, and so on.
It was intended to be insulting. good. There is no free lunch....etc. Either come up with new and innovative ideas and market them to the right place or sit back and whine.
} } ... you are dead wrong. The innovation and initiative is done long } } before it reaches the CBD. If you do not innovate and create and } } seek sources for your products and services, you are not likely to } } have a good day. } } My suspicion is that the RFP's were fishing for free ideas.
Nope.
} } "You have to kiss a lot of frogs to find a prince." } } No thanks; there are no Princes in that pond.
You have not kissed many frogs I take it?
} } } I would encourage you to better understand how the system works } } before you criticize it. } } Thought I did. Had two long-term, innovative research projects } which I thought I had won fair & square when I taught at Drexel } University.
OK...so what happened?
} } Remember, this system is the product of "our government," and } } as such, it is supposed to equalize dealings of the dispensation } } of taxpayer funds. } } That was what I was sour graping about. Didn't seem fair to me - } all one way, with no feedback. And I haven't begun to sound off } about the way the NSF treated research-fund seekers in the } universities. Too many proposals chasing too little funding, with } the "riches" going to the most persistent (read: repetitive) proposal } writers, who would then use that funding to support yet more proposal } writing. A gigantic Ponzi scheme, but in reverse. Forgive me if } it's all been changed in the last eighteen years since I participated } in that circus.
Over 10 years, I sponsored and funded upwards of 15 SBIRs while I was a gov engineer. Nearly all of these went to Phase II. In all, I think that I invested about $15M of government funds on SBIRs. some did pay off. some did not. But research is not an area of guarantees. The one with the money decides where those dollars are to go. Your job is to convince him/her that your venue is the right place.
} Best regards, } George Langford, Sc.D., in SE PA, happily doing consulting for } folks who actually want ideas & answers for their problems and } who pay in real dollars (no overhead fudge factors) for the } work we actually do for them and for what we said we'd charge. } amenex-at-amenex.com } http://www.amenex.com/
The government auditors will be there to make sure that what you say and what you do are in harmony.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Modern surfers use PC boards. You can too at http://photoweb.net ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
I know this has been talked about quite a few times, but on checking the archives I can't find quite what I want. Nor have I been able to track down an answer on the Web. In my Dept we have a cheap Epson and a networked B&W laser, but would like to get a moderately fast, networkable photo quality printer that can also do routine colour printing. We can't afford a Pictrography, but want more than an ink jet. Are there any laser printers with photo quality? If the pics don't turn out as well as the Epson, then it's not good enough. It would be nice if the prints were long lasting and not too expensive as well! Could I have some suggestions (and approx prices) please?
Thanks all,
Diana
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
Gary & I are having an unusually civil Internet Fight about The System, how it says it works (GL), and how it actually runs (GG).
If I get the gist of Gary's position, it is that the published RFP's and all such announced intentions and WTB's by The Guv'mint are done deals, put in those venues (like death notices) to satisfy legal niceties. And that the real business of Guv'mint is done between lobbyists, whose business it is to walk the streets of Washington, pounding on doors & grabbing at collars, and the funding fathers. Writers of RFP's, like the payers of taxes, are just the air & water flowing past those high offices.
This has a chilling parallel to Hernando de Soto's, The Other Path, which describes how Peruvians learned to deal with their Guv'mint, except that everyones' roles now seem to be interchanged. In Peru, the simple folk learned simply to squat on land that was idle & unused and then to repeat the process over & over again when evicted, until the Guv'mint grew tired. Then they started building their own system of government, their own infrastructure, and even their own courts. All outside The System. Here we have a system in which all proposals, contracts, and the like are open to public scrutiny under the Freedom of Information Act, and Full Disclosure demands publication of RFP's in the CBD, leading to such a barrage of proposals and reviews that another system quietly replaces it, one in which the best ideas are traded for funding quietly, behind the scenes.
What's needed is a new system for funding research that does away with both the mountainous paperwork and the lobbying. My solution was to discard any further consideration of Public Funding and to become a Capitalist in the Free Market. Amenex did win one good one - from the Ben Franklin fund in Pennsylvania, for an idea that was good enough to become commercialized and which became a standard product, copied worldwide. We lost money on it, though, but the idea won out ... it wasn't mine, but I understood it better than the inventor ... and it was fun to try to make the business portion of the enterprise work. We even ended up with a new microscope out of the deal (capital expenditures were allowed back then), so I haven't strayed off topic that much.
And the Guv'mint's auditors haven't bugged us a bit.
Best regards, George Langford amenex-at-amenex.com http://www.amenex.com/
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At 07:06 PM 11/22/99 , you wrote: } Hi Gary & bystanding Microscopists ! } } Gary & I are having an unusually civil Internet Fight about The } System, how it says it works (GL), and how it actually runs (GG).
yes.
} If I get the gist of Gary's position, it is that the published RFP's } and all such announced intentions and WTB's by The Guv'mint are done } deals, put in those venues (like death notices) to satisfy legal } niceties. And that the real business of Guv'mint is done between } lobbyists, whose business it is to walk the streets of Washington, } pounding on doors & grabbing at collars, and the funding fathers. } Writers of RFP's, like the payers of taxes, are just the air & water } flowing past those high offices.
It may seem that way.
} This has a chilling parallel to Hernando de Soto's, The Other Path, } which describes how Peruvians learned to deal with their Guv'mint, } except that everyones' roles now seem to be interchanged. In Peru, } the simple folk learned simply to squat on land that was idle & unused } and then to repeat the process over & over again when evicted, until } the Guv'mint grew tired. Then they started building their own system } of government, their own infrastructure, and even their own courts. } All outside The System. Here we have a system in which all proposals, } contracts, and the like are open to public scrutiny under the Freedom } of Information Act, and Full Disclosure demands publication of RFP's } in the CBD, leading to such a barrage of proposals and reviews that } another system quietly replaces it, one in which the best ideas are } traded for funding quietly, behind the scenes.
This is not Peru. We do not dress their way nor practice cultural rituals their way. so parallels to anywhere else are irrelevant. They may be interesting but either way, they are not relevant to our own reality.
} What's needed is a new system for funding research that does away } with both the mountainous paperwork and the lobbying. My solution } was to discard any further consideration of Public Funding and to } become a Capitalist in the Free Market. Amenex did win one good } one - from the Ben Franklin fund in Pennsylvania, for an idea that } was good enough to become commercialized and which became a standard } product, copied worldwide. We lost money on it, though, but the idea } won out ... it wasn't mine, but I understood it better than the } inventor ... and it was fun to try to make the business portion of the } enterprise work. We even ended up with a new microscope out of the } deal (capital expenditures were allowed back then), so I haven't } strayed off topic that much.
OK...so what is your argument? Based on what you have said so far, I would have not have funded your SBIR.
Interestingly, there are numerous small businesses with great ideas who engage the SBIR establishment. Some of them win. Those that do generally go on to venture capital for the "big time" or fail due to poor management skills or just a plain bad idea. One key factor in most SBIRs is that small businesses are poorly managed. This does not mean that the businesses are bad. Nay. It means that the technical folks are speaking while the business folks are either absent or silent. When the dust settles, the techo-babble has worn off and the business end is lacking. Hence, no SBIR.
We are doing a lot of highly automated micrscopy and advanced image analysis here at the company. We mainly use SCIL Image 1.4 for the development of our algorithms and applications.
Although SCIL image 1.4 is a very powerful environment for developing image analysis applications, I have never seen other companies, doing microscopy, that published articles/applications in which they used this software development environment.
I have the idea that most morphologists still use packages like NIH-image. These packages are fine for doing low-volume image analysis, but lack the powerful image processing capacity of software like SCIL Image.
Besides a few Universities in the Netherlands, the amount of users seems very limited. Are there people on this list, who also use SCIL Image for their image analysis ? If there are people out there who use SCIL image 1.4, do they intend to switch to its sucessor HORUS (JAVA/C++) in the near future ?
SCIL Image 1.4 comes with an interface (SCIL) and a C-libray for image analysis (Image 2.1). When you know how to program in C, you can use SCIL Image.
Senior Scientist Medical Image Analysis Biological Imaging Laboratory Life Sciences Department I - 6065 Janssen Research Foundation Turnhoutseweg 30 B-2340 Beerse Belgium Europe
Hi Jim. Have a look at "4H Glove (TM)". They are useable for protection against a lot of very different chemicals an a rather thin (multilayer foil, not for "hard" work). From 1996 I have also this net address: www.safty4.com/guide/set_guide.htm Some of the chemical you use seem to be not on this list, but you should ask them directly.
Disclaimer: I have no financial interest of any kind in this product or company. Regards Gerhard Frank
JIM ROMANOW wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Can someone please help me determine, or direct me to a comprehensive guide } for determining, the appropriate gloves for handling each of the following } chemicals? } } Epoxy Resin } Propylene Oxide } Glutaradehyde } Formaldehyde } Acetone } Osmium Tetroxide } Ethanol } Cacodylate } Uranyl Acetate } Lead Citrate } } I have talked with our environmental health and safety officer and read the } permeation/degradation, resistance guides in our chemical and industrial } safety vendor catalogs. Some of our chemicals are not on any company's } glove guide but several of the ones that are require using a bulky heavy } duty type glove made from Viton, Butyl, Neoprene or Norfoil and best suited } for gross chemical handling. Is there a U.S. distributor for form fitting } flexible gloves made from these or equivalent materials? } } Thank you. } } James S. Romanow } The University of Connecticut } Physiology and Neurobiology Department } Electron Microscopy Facility } U-2131 } Storrs, CT 06269-2131 } bsgphy3-at-uconnvm.uconn.edu } 860 486-2914 voice } 860 486-1936 fax
-- Gerhard Frank UNIVERSITAET ERLANGEN-NUERNBERG Phone: +49 9131 85 28606 Institut fuer Werkstoffwissenschaften VII Fax: +49 9131 85 28602 Cauerstr. 6, D-91058 Erlangen, Germany frank-at-ww.uni.erlangen.de
I have a client with an urgent need of the above for some QA work on polymer films (i.e. rapid non destructive non contact measurements of thickness variations). Although it's slightly off topic, I would be interesting in hearing from ayone who could help us out, especially in Europe.
I am increasingly being asked to prepare bacterial suspension cultures (E. coli mutants, mostly) for TEM sections. Not being a bacterial person (I prefer my organisms to come in lumps that need to be cut up) I would be grateful for advice on the preferred method of processing bacterial cells, particularly in suspension cultures, but I would also be interested in tips for dealing with individual colonies on agar.
Thanks in advance Chris Jeffree ===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
I would deeply appreciate it, if you could provide me with valuable info regarding your views on ion beam thinning equipment available for TEM preparation.
In particular:
a) Does anyone has any negative strong views regarding a particular make or model?
b) Is there any make /model that you would recommend for a multiuser environment (preparation of metallic, composites and ceramic samples)?
c) Any views or experiences on support and services after purchasing of a particular model/equipment
d) Any comments on initial investment costs over the life of particular makes/models
Feel free to reply either on the list or off the list.
We inherited a Reichert-Jung Lynx el tissue processor several years ago. While it seems like a nice machine we have never used it. Free to a good home--you pay shipping. Please e-mail me directly.
Alps makes a dye sub printers which are very good. We have the MD 1300. The photo quality images are excellent, but they require the special paper and ribbons from Alps. These printer are not very expensive ( { $400), but they are relatively slow (5 to 10 minutes /print). The Epson Stylus Photo printers are much faster, but have a dottier image. Bill
William McManus Supervisor Electron Microscopy Facility Department of Biology Utah State University Logan UT 84322-5305
billEMac-at-cc.usu.edu 435-797-1920
-----Original Message----- } From: Diana van Driel [mailto:dianavd-at-eye.usyd.edu.au] Sent: Monday, November 22, 1999 7:09 PM To: MicroscopyList
I know this has been talked about quite a few times, but on checking the archives I can't find quite what I want. Nor have I been able to track down an answer on the Web. In my Dept we have a cheap Epson and a networked B&W laser, but would like to get a moderately fast, networkable photo quality printer that can also do routine colour printing. We can't afford a Pictrography, but want more than an ink jet. Are there any laser printers with photo quality? If the pics don't turn out as well as the Epson, then it's not good enough. It would be nice if the prints were long lasting and not too expensive as well! Could I have some suggestions (and approx prices) please?
Thanks all,
Diana
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
I would like to hear from any electron microscopists out there who have had personal hands on experience with the RMC Propane Jet Freezer, model MF-7200. I am considering using one to rapidly freeze cells in suspension. Any advice out there?
Thanks,
Timothy Schneider, Director of Electron Microscopy Thomas Jefferson University 215-503-4798
} Can someone please help me determine, or direct me to a comprehensive guide } for determining, the appropriate gloves for handling each of the following } chemicals? } } Epoxy Resin } Propylene Oxide } Glutaradehyde } Formaldehyde } Acetone } Osmium Tetroxide } Ethanol } Cacodylate } Uranyl Acetate } Lead Citrate } } } I have talked with our environmental health and safety officer and read the } permeation/degradation, resistance guides in our chemical and industrial } safety vendor catalogs. Some of our chemicals are not on any company's } glove guide but several of the ones that are require using a bulky heavy } duty type glove made from Viton, Butyl, Neoprene or Norfoil and best suited } for gross chemical handling. Is there a U.S. distributor for form fitting } flexible gloves made from these or equivalent materials? } } James S. Romanow
This is an important question, and we need more solid, documented information. Tobler & Freiburghaus recommend bulky, clumsy, expensive "4H" gloves for methacrylates [J. Microscopy 160:291-298(1990)], with latex in 2nd place & vinyl 3rd. Ringo, Read, & Cota-Robles [J.E.M. Technique 1:417-418(1984)] found clumsy, cheap polyethylene much better than latex in resistance to epoxy monomers, with vinyl again a poor 3rd. I've heard comments that "nitrile is good", but I haven't found any data yet. If anyone is aware of an ideal glove, I'm sure that at least one of our excellent supply houses will be happy to offer it!
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
When processing difficult to embed specimens for TEM vacuum infiltration is often recommended for the resin infiltration steps. However, a lack of specfic technical detail seems to often accompany this recommendation. Missing detail includes; What pressure should be applied for vacuum infiltration ? How long should this pressure be applied ? Should the vacuum infiltration procedure be applied to the propylene oxide/ resin steps and pure resin infiltration steps, or just to the pure resin infiltration steps ? Can applying a vacuum be useful for any other of the processing steps, for instance, primary fixation ? Is so, what pressure and how long ?
Many thanks in advance for all responses.
Allan ----------------------------------------------------------------------------= ----
Replies;
To: allan.mitchell-at-stonebow.otago.ac.nz =46rom: Steve Beck {becks-at-sunynassau.edu
Dear Allan, I usually vacuum infiltrate using a small rotary pump and a bell jar setup. During 1:1 propylene oxide/pure mixed resin (I mostly use an Epon - Araldite mixture) for an hour, I place the mixed resin (in a plastic beaker/tri-pour) under the vacuum to degas it (incredible how much air is introduced during the 15-20 minute manual mixing we do). I then take the degassed resin and using a plastic Pasteur pipette, I fill the bottom of a small Petri dish (6cm diameter?). After the hour in the 1:1 propylene oxide/resin, I use a bamboo stick to transfer tissue blocks into the Petri dish. The dish is left uncovered and placed into the bell jar and evacuated. I vacuum infiltrate for 1 hour. During this time, I place labels into my BEEM capsules and fill them to a slight negative meniscus with the degassed resin. After vacuum infiltration I use the bamboo sticks to transfer tissue blocks to the top center of the BEEM capsule filled with resin and then I place them into an incubator (48 hours -at- 60=9AC). Before polymerization, the heavy osmicated blocks sink to the tip of the BEEM capsule.
I have never had problems with air bubbles or poor embeddments with this method.
Hope this helps!
P.S. email me tomorrow if you need to know the ultimate vacuum of my rotary pump - I can get the info. when I'm in my lab!
To: allan.mitchell-at-stonebow.otago.ac.nz =46rom: L R MELSEN {lmelsen-at-emory.edu
Allan, We use this routinely for monolayers and bacterial samples. The vacuum is applied during the final 100% step fror 15 minutes, the 1:1 step 2 hour to over night, and the full strength step for 4 hours. The negative pressure is about 14lbs. on our house vacuum lines.
Regards, Skip
----------------------------------------------------------------------------= ---- To: Richard Lander {richard.lander-at-stonebow.otago.ac.nz =46rom: Mary Mager {mager-at-inch.interchange.ubc.ca
Dear Richard, My experience with vacuum infiltration is all with epoxy mounting in materials specimen prep., but it may be some help in your work.
The purpose of vacuum infiltration is to get air out of the sample and use the resultant vacuum to suck resin into the fine spaces and holes in the material. However, all the materials being used in the emedding are liquids which will boil if the pressure is dropped too low. I use a vacuum dessicator with a three-way valve connected to an old vacuum pump I don't care much about. The vapors you will be puting into the pump are quite hard on it. I put the material in epoxy molds into the dessicator, which has a clear lid. Watching carefully , I start the pump and turn the valve to start sucking on the dessicator. You will see a bit of vapor appear, then some bubbles will come up to the surface of the epoxy. Then the epoxy will start to foam slightly. At this point I turn the valve to admit air. I usually repeat this three times.
I would not use this technique on any liquid with a high vapour pressure or low boiling point, but it should work to get intimate contact between any liquid and solid.
----------------------------------------------------------------------------= ---- To: Richard Lander {richard.lander-at-stonebow.otago.ac.nz =46rom: Michael Pidgeon {pidgeon-at-hsc.usc.edu
Allan:
generally you use a gentle vacuum, 20-30 Microns for the same length of tim= e you use for standard infiltration. If you use LR White, it is not advisable to go beyond 30 minutes per change in a vacuum as it tends to begi= n polymerization. You would include any of the 1:1 dilutions of resin and propylene oxide or resin and EtOH, in the vacuum infiltration process.
Yes, vacuum can help the fixative to pentrate into stubborn specimens during primary fixation.
If you have any further questions, feel free to email me.
To: Richard Lander {richard.lander-at-stonebow.otago.ac.nz =46rom: MICHAEL DELANNOY {delannoy-at-welch.jhu.edu
Richard, we routinely do vac infilt on insect and hard to penetrate samples (bone, plant etc). We use a room temp vac oven at 15 psi with the caps open and only with pure resin (no p.o.) for a couple of hours, change plastic and infiltrate further at atmospheric pressure, change again and back to the oven for 15 psi then turn oven on to warm to 60 degrees c overnight. I have never done vac fix infiltration, but might work, I would run side by side atmospheric fix for comparsions. Let me know, good luck
------------------------------------------------------------ Allan Mitchell Technical Manager South Campus Electron Microscope Unit C/-Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
,,, (o o) ------------------oOO-(_)-OOo----------------------------------
----------------------------------------------------------------------- Richard Lander Electron Microscopist South Campus Electron Microscope Unit Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand. Tel. National 03 479 7301 Fax. National 03 479 7254 mailto:richard.lander-at-stonebow.otago.ac.nz http://www.otago.ac.nz/anatomy/emunit/ ------------------------------------------------------------------------
I think it worth revisiting this topic from time to time since the technology and prices seem to change fairly often in this very competitive market.
For what it is worth, I just bought a Hewlett Packard 970Cxi ($399), their latest business-class inkjet printer and it does a very nice job on high quality paper--better than the Epson's IMHO. It is also fast and networkable. Publication quality? I'll let you know soon.
Gary P. Radice gradice-at-richmond.edu Associate Professor of Biology 804 289 8107 (voice) University of Richmond 804 289 8233 (FAX) Richmond VA 23173 http://www.science.richmond.edu/~radice
At 9:42 AM -0500 11/23/99, Timothy Schneider wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
************* Try Leica, they have the rights.
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Confocal Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
} For details of PPE (personal protective equipment) I sometimes find the } websites of manufacturers or suppliers useful, for example: } } http://www.marigoldindustrial.com/range/index.html } } Robert H.Olley } J.J.Thomson Physical Laboratory } University of Reading
Dr. Olley -
The website that you cite reinforces my call for specific information. It emphasizes protecting products from the glove wearer, and provides no specific information on the protection provided TO the wearer. The other catalog references suggested by others today are just as uninformative. We work with chemicals (resin monomers are a good example) that will penetrate many gloves, and we work at a small scale so the original inquiry for something that fits well is reasonable. 4H gloves are resistant, but they're clumsy (and expensive); does anyone have specific information about an alternative?
Caroline
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
I am getting in on this thread late, so I hope I'm not rehashing old points here, or re-stating the obvious.
The recommended gloves for some of these chemicals makes dexterity difficult or non-existent.
I applaud your efforts to get the appropriate chemical resistance in your personal protective equipment (PPE). However, be careful of over-kill. In our use of many of the chemicals that you mention, we use nothing more than two layers of 4 or 8 mil nitrile gloves. However, we take extra care to engineer our lab procedures to minimize potential exposure, and potential duration of exposure. For example in many cases, we do not pour the solution or solvent, but instead we draw it from a container with a blunt end pipette or syringe. The chemical compatibility charts are assuming full submersion and are rated based on the gloves time to permeation/deterioration and even break-through. We rarely have a lab procedure that requires any chemical contact with the gloves. And often, using the planned precautions, we opt for use of a thinner, higher dexterity glove that may give less than a maximum level of protection, but then we use frequent checks and changes of the gloves to prevent permeation or break-through. This allows us to continue to work safely with dexterity, handling the nasty chemicals. Sometimes the PPE can be so cumbersome that the materials can no longer be handled safely, or in other words, the PPE may become the "real hazard". We use experimental design to minimize the risk, and the need for maximum protection, then go to the charts to determine the level of protection required. Good Luck Brad
} Can someone please help me determine, or direct me to a comprehensive guide } for determining, the appropriate gloves for handling each of the following } chemicals? } } Epoxy Resin } Propylene Oxide } Glutaradehyde } Formaldehyde } Acetone } Osmium Tetroxide } Ethanol } Cacodylate } Uranyl Acetate } Lead Citrate } } I have talked with our environmental health and safety officer and read the } permeation/degradation, resistance guides in our chemical and industrial } safety vendor catalogs. Some of our chemicals are not on any company's } glove guide but several of the ones that are require using a bulky heavy } duty type glove made from Viton, Butyl, Neoprene or Norfoil and best suited } for gross chemical handling. Is there a U.S. distributor for form fitting } flexible gloves made from these or equivalent materials? } } Thank you. } } James S. Romanow
Nestor, I know you declared a truce but I'm sorry I feel I have to do this to "clear" my name/reputation.
I have been watching the board amazed at the reaction by everyone to my request for a specialist. For those of you who know me, it is a sincere attempt to find a qualified individual. Yes it is very specific. No, it is not a "bag job".
I am aware of a few groups around the world who do this sort of work and I was honestly trying to elicit a response from those individuals. I did contact several of those people directly but felt that the board represents a unique opportunity to reach out to those who may no longer be associated with the respective research groups. I was always under the impression that this was one of the major advantages of the board and premise for it's existence. I'm sorry that I have caused this level of "needless" discussion on the database.
We are interested in recommendations for a hotstage for a light microscope. If there is cooling capabilities that will be an added plus, if it doesn't compromise the heating function. Our Mettler FP5 finally failed after faithfully servicing us for 25 years. We have looked at the Mettler FP82, seen the Linkam and heard about an Instec (HCS600). Any experience with these would be appreciated. Vendors are welcome to contact me directly at: GaroneL-at-Polaroid.com Thanks, Lynne Garone Polaroid Corp.
I am seeking out a replacement keyboard for a Bio-Rad Digilab 3240 SPC FTIR. Our keyboard has been gradually dying over the last few months and I am hoping somebody out there has one that I might be able to convince them to sell. The system is vintage early to mid 80's (I think) so perhaps there exists one in an instrument graveyard/backroom? The system is not PC based and the keyboard has a variety of peculiarities that would seem to preclude the use of a standard keyboard (function keys and built in joystick for starters). All suggestions happily entertained.
Thanks to everyone in advance, Doug Seitz
Engis Corporation Wheeling, IL 60090
"The opinions expressed above are mine and mine alone...for who else would own up to them?"
As always seems to be the case, I end up reserving more rooms than I end = up needing. It also seems that many of you are looking for hotel rooms at t= he last minute. With that in mind, I do have 3 rooms available for the Marriott at Copley Place for arrival November 27 and departure December 3= . =
The rates are approx. $130 Single, $145/double. These are currently reserved as king bed rooms. If anyone would like to "claim" these rooms before I cancel my reservations, please email me ASAP. I will cancel my reservation on Wednesday night so, if you need them, let me know now! Yo= u may be able to switch them to rooms with 2 double beds, but no guarantees=
I am aiming to preserve and visualise oils in the rind of orange fruit during tissue processing for LM and TEM examination.
I am currently using an osmium postfix with glut/para fixation, acetone dehydration, epon araldite embedding. Sudan Black B stain is used for LM and UA and LC for TEM.
Cryo techniques may be an option - I have trialled cryofixation (slam) and freeze substitution, but need to include osmium in the method or a lipid stain (?) to visualise the oil under TEM.
Any suggestions would be greatly appreciated, cheers.
At 12:36 PM 11/23/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Ink jets are nice but not the end-all. Dye sublimation is best. But it is not cheap. Laser color is a good compromise. The new HP Color LaserJet 4500 is rather good. it does raster and Postscript. I'm seeing prices between $1500 and $2000 without a network card. You might want to check this out. QMS also has a nice color laser printer. the pricing is in the same ballpark.
I compare these to my Kodak DS 8650 PS dye sublimation printer. This is a high standard. the lasers do a good job. I tend to prefer the HP.
I am looking at the rind oils of orange fruit with LM and TEM.
I am currently using an osmium postfix with glut/para fixation, acetone dehydration, epon araldite embedding. Sudan Black B stain is used for LM and UA and LC for TEM.
Cryo techniques may be an option - I have trialled cryofixation (slam) and freeze substitution, but need to include osmium in the method or a lipid stain (?) to visualise the oil under TEM.
Any suggestions would be greatly appreciated, cheers.
} -----------------------------------------------------------------------= - } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------. } } Hello: } } I am looking for contact information (web site or phone #) for LKB } ultramicrotomes and related equipment. } } Thanks, } } Timothy Schneider, Director of Electron Microscopy } Thomas Jefferson University } 215-503-4798
LKB do not exist anymore. The service for LKB ultramicrotomes is run by Leica, at least in Germany, but I think worldwide and they might give you the information.
-- Dr. Anne Heller, AG Elektronenmikroskopie, Institut f=FCr Botanik (210), Universit=E4t Hohenheim, Garbenstra=DFe 30 D-70593 Stuttgart
I would rinse the bacteria (from broth culture) in a buffer they like, spin, fix and pellet in an Eppendorf. Usually the bacteria will stay in visible clumps which settle rapidly and can be treated in the same way as tissue.
Dave
On Tue, 23 Nov 1999 11:04:45 +0000 Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear All } } I am increasingly being asked to prepare bacterial suspension } cultures (E. coli mutants, mostly) for TEM sections. } Not being a bacterial person (I prefer my organisms to come in } lumps that need to be cut up) I would be grateful for advice on the } preferred method of processing bacterial cells, particularly in } suspension cultures, but I would also be interested in tips for } dealing with individual colonies on agar. } } Thanks in advance } Chris Jeffree } ===================================================================== } DR CHRIS JEFFREE } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY } UNIVERSITY OF EDINBURGH } Daniel Rutherford Building } King's Buildings, Mayfield Road } EDINBURGH, EH9 3JH, Scotland, UK } Tel. #44 131 650 5345 } FAX. #44 131 650 6563 } Mobile 0410 585 401 } email c.jeffree-at-ed.ac.uk } SEM / TEM bookings sem-at-ed.ac.uk } ===================================================================== }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
Mostly we aim to stain the specimen, rather than the resin, but I have a particular problem where the specimen cannot be stained, but might become visible in negative contrast. Does anyone know of a neat way of staining the RESIN in a TEM section so that it becomes electron dense and provides negative contrast? Alternatively, is there an electron dense resin that can be used for TEM, or is there a way of making a standard epoxy resin mix somewhat more electron-dense than normal?
Chris Jeffree ===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
Instead of using acetone for dehydration try acidified dimethoxypropane. It is a chemical dehydration and for small pieces of plant material 15 min dehydration is usuallly enough.
If you want to use cryo techniques. Start to substitute in acetone at -80, repalce acetone with new acetone containing 1% OsO4 and raise the temperature to -50 - -40. It is essential that the acetone OsO4 mixture is kept below -60 until you raise the temperature. Leave the tissue in the mix for more than 2 h and embedd in Lowicryl.
Using this technique you should be able to retain the lipids in the tissue.
Bo
Toby Knight wrote: } I am looking at the rind oils of orange fruit with LM and TEM. } } I am currently using an osmium postfix with glut/para fixation, acetone } dehydration, epon araldite embedding. Sudan Black B stain is used for LM } and UA and LC for TEM. } } Cryo techniques may be an option - I have trialled cryofixation (slam) } and freeze substitution, but need to include osmium in the method or a } lipid stain (?) to visualise the oil under TEM. } } Any suggestions would be greatly appreciated, cheers. } } -------------------------------------------------------- } Toby Knight } PhD student } Email: tknight-at-waite.adelaide.edu.au } --------------------------------------------------------
The tombac of the anticontamination device of our Siemens Elmsikop 102 TEM is defect. It generates a microleak and the TEM is out of order.
We are looking for a complete anticontamination device (cooling finger + cooling vessel) for this kind of microscope (Elmiskop 102).
Can anyone help us ?
Thanks in advance
Philippe Drouillon Solvay Research and Technology Electron Microscopy and Image Analysis - Coordinateur Informatique de Division Rue de Ransbeek, 310 B-1120 Brussels (Belgium) phone : (00 32) 2 264 24 47 mailto:philippe.drouillon-at-solvay.com
To All, I am interested in hearing from those of you who can offer me some suggestions on the sectioning of papers, specifically coated papers or those papers upon which wet (ink-jet inks) or dry (toner) forms of printing have been completed.
Typically we overcoat the paper surface, i.e. sputter a film of metal then embed the paper sample in epoxy. Afterwards we have prepared thick sections (0.5 - 0.8 microns) without water in the boat using either a glass or diamond knife followed by examination using a light microscope. To examine these samples using the TEM requires thinner and non-distorted sections. Obviously sectioning with water in the boat swells the paper, possibly interfering with the coating on the paper.
Anyone having experience in this area or that can offer suggestions I would appreciate hearing from.
Regards, Paul
Paul J. Gerroir Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
To anyone that is interested in Propane Jet Freezers formerly manufactured under the RMC brand.
These are now manufactured by Ventana Medical Systems, Inc.
The NEW JF-8000 will be at the Cell Biology meeting in Washington, D.C. on December 12-15, booth 567. Al Coritz, cryoproducts Product Manager will be in attendance to answer questions.
Ventana Medical purchased RMC in October 1998, all manufacturing, marketing and servicing is now conducted by Ventana. For complete literature and specifications please contact me.
Steven W. Miller North American Sales Manager Microscopy Group Ventana Medical Systems, Inc. 3865 N. Business Center Dr. Tucson, AZ 85705 Smiller-at-Ventanamed.com Phone: 520-690-2753 Fax: 520-690-3580 Web site: www.Ventanamed.com look for the Ventana/RMC link on the bottom of the Home Page.
I have the electronics, terminal, and camera for a Kevex uX-system7000. If you want it, then drop me a line. The detector is not up for grabs.
Pickup......it is yours
Jim
********************************************************* Dr. Jim Quinn jquinn-at-doL1.eng.sunysb.edu Materials Science 631-632-6663 FAX:8052 SUNY at Stony Brook http://doL1.eng.sunysb.edu/ Stony Brook, New York 11794 - 2275 *********************************************************
Dear George, I have had the VCR Ion Mill for five years now. I have been very pleased with it and it always gives me excellent service. It has both ion and atom modes and other components available for reactive ion milling. It has a third ion gun for termination, which has proved valuable for terminating samples with optically transparent components e.g. Al2O3 in Al. I have always gotten quick and correct response from the company to solve any problems and we have been very happy with the experience. I thin metal matrix composites, clean the oxide off of electropolished samples, thin rocks for Geology and thin semi-conductors. The VCR company has been joined with the South Bay recently, but the people from VCR are now with South Bay, so the quality and service should remain the same. At 12:39 PM 11/23/99 +0000, you wrote: } } Dear Colleagues } } I would deeply appreciate it, if you could provide me with valuable info regarding your views on ion beam thinning equipment available for TEM preparation. } } In particular: } } a) Does anyone has any negative strong views regarding a particular make or model? } } b) Is there any make /model that you would recommend for a multiuser environment (preparation of metallic, composites and ceramic samples)? } } c) Any views or experiences on support and services after purchasing of a particular model/equipment } } d) Any comments on initial investment costs over the life of particular makes/models } } } Feel free to reply either on the list or off the list. } } } I would deeply appreciate your valued comments. } } } Regards } } } George } Regards, Mary Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
microscopy in a Neuroscience Lab. Duties to include: ultrathin serial sectioning; serial section analysis using electron microscope; post-embedding immunocytochemistry; photography-developing and processing of prints; stock supplies, keep inventory; develop new techniques: conduct literature searches; test alter and implement new methods to improve lab procedures and maximize productivity; requires results/data interpretation; will provide training to research personnel in photographic and electron microscopic techniques; the individual will assist in the preparation of scientific paper for publication; will independently: prepare tissue for electron micrographic examination, examining tissue, analyzing data, and preparing data for publication. Comply with governmental regulation and University policies regarding health and safety and observe and support health and safety practices including those specifically related to the work environment.
QUALIFICATIONS: Bachelor's degree in a related field is required; master's degree preferred; plus 3 years of applicable experience. Must be able to work effectively in a team and independently. Must exercise considerable judgement, initiative, and resourcefulness. Must be able to lift up to 50 lbs. Fluent in written and verbal English. Basic knowledge in use of personal computers, photographic equipment, and electron and light microscopes.
Salary range: $3404-4544. Exempt. New position. For more information email Dr. Buckmaster at pbs-at-leland.stanford.edu
Send resumes to:
Dr. Paul Buckmaster Department of Comparative Medicine Building 330, Quad 7, RAF-1 Stanford University Stanford, CA 94305
Chris, While I don't do TEM work, I have used several epoxies in other applications. There are brominated equivalents used with or instead of the Shell Epon series (for flame retardant purposes) and I have used some from Great Lakes Chemical, I think they are at 317-497-6100, Lafayette, IN, USA. Perhaps someone there may be able to suggest a brominated epoxy that will "drop into" your epoxy protocol (EEW numbers would be a good start) assuming bromine is heavy enough for your application.
Dave Audette OSRAM Sylvania 71 Cherry Hill Drive Beverly, MA 01915 david.audette-at-sylvania.com
I don't have any interest in Great Lakes Chemical etc.
} -----Original Message----- } From: Chris Jeffree [SMTP:cjeffree-at-srv0.bio.ed.ac.uk] } Sent: Wednesday, November 24, 1999 6:18 AM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Electron dense resins?? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Mostly we aim to stain the specimen, rather than the resin, but I } have a particular problem where the specimen cannot be stained, } but might become visible in negative contrast. } Does anyone know of a neat way of staining the RESIN in a TEM } section so that it becomes electron dense and provides negative } contrast? Alternatively, is there an electron dense resin that can } be used for TEM, or is there a way of making a standard epoxy } resin mix somewhat more electron-dense than normal? } } Chris Jeffree } ===================================================================== } DR CHRIS JEFFREE } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY } UNIVERSITY OF EDINBURGH } Daniel Rutherford Building } King's Buildings, Mayfield Road } EDINBURGH, EH9 3JH, Scotland, UK } Tel. #44 131 650 5345 } FAX. #44 131 650 6563 } Mobile 0410 585 401 } email c.jeffree-at-ed.ac.uk } SEM / TEM bookings sem-at-ed.ac.uk } =====================================================================
I was pleased to see the posting by Mary Mager and to read her kind words= . =
As she said, VCR Group has been acquired by South Bay Technology and we a= re now producing the XLA 2000 Ion Mill (as well as the DIMPLER=AE and the Io= n Beam Sputter Deposition and Etching System). =
We are actually offering two ion milling systems that may be of interest:=
1) IV3 Low Energy Ion Milling System This system offers 2 guns (gun #1 2-10kV; Gun #2 200V to 2kV) and reduce= s or eliminates amorphous damage to samples through *effective* low energy milling without compromising overall milling rates.
2) XLA 2000 Ion Milling System (formerly produced by VCR Group, inc.) This is a computer controlled system offering a 3 gun configuration, faraday cup termination, LN2 cooling etc. Ideal for multi-user installations.
They both have unique features which distinguish them from any other systems on the market and are well worth your consideration.
I will contact you off-line with more details and I encourage anyone else=
who has an interest in ion milling systems to contact me off-line for additional information.
} } } } } Please visit us at http://www.southbaytech.com { { { { {
Manufacturers of precision sample preparation equipment and supplies for metallography, crystallography and electron microscopy.
Message text written by "G. Fourlaris" } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
Dear Colleagues
I would deeply appreciate it, if you could provide me with valuable info regarding your views on ion beam thinning equipment available for TEM preparation.
In particular:
a) Does anyone has any negative strong views regarding a particular make = or model?
b) Is there any make /model that you would recommend for a multiuser environment (preparation of metallic, composites and ceramic samples)?
c) Any views or experiences on support and services after purchasing of a=
particular model/equipment
d) Any comments on initial investment costs over the life of particular makes/models
Feel free to reply either on the list or off the list.
I have had good results recently with a stage that cools and heats made by: Brooks Industries 25570 Lehman Blvd. Lake Villa, Il 60046-6300
847-356-1045
} We are interested in recommendations for a hotstage for a light microscope. } If there is cooling capabilities that will be an added plus, if it doesn't } compromise the heating function. Our Mettler FP5 finally failed after } faithfully servicing us for 25 years. We have looked at the Mettler FP82, } seen the Linkam and heard about an Instec (HCS600). Any experience with } these would be appreciated. } Vendors are welcome to contact me directly at: } GaroneL-at-Polaroid.com } Thanks, } Lynne Garone } Polaroid Corp.
Larry D. Ackerman Lily & Yuh Nung Jan Laboratories Howard Hughes Medical Institute UCSF, Box 0725, Rm U226 533 Parnassus Ave. San Francisco, CA 94143
You could try dehydrating with Aquembed from Ladd. It's very expensive and a little awkward to use, but it will leave oils untouched - it's a water-miscible epoxy resin. You can also embed in it, but it makes a rather soft block. For your purposes soft may be good, of course. Hope this helps.
Lesley Weston.
On Wed, 24 Nov 1999, Toby Knight wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hello, } } I am aiming to preserve and visualise oils in the rind of orange fruit } during tissue processing for LM and TEM examination. } } I am currently using an osmium postfix with glut/para fixation, acetone } dehydration, epon araldite embedding. Sudan Black B stain is used for LM } and UA and LC for TEM. } } Cryo techniques may be an option - I have trialled cryofixation (slam) } and freeze substitution, but need to include osmium in the method or a } lipid stain (?) to visualise the oil under TEM. } } Any suggestions would be greatly appreciated, cheers. } } -------------------------------------------------------- } Toby Knight } PhD student } Email: tknight-at-waite.adelaide.edu.au } -------------------------------------------------------- } } }
Chris, You might try adding lead methacrylate, or other similar organo-metalic, to an epoxy such as Spurrs, which worked for us years ago. It would likely work as well, if not better, with the present day acrylics. Don Strickler published a paper (I believe in Om Johari's SEM journal, 1980's?), in which he described this technique to increase the electron density of Spurrs in order to enhance contrast of embedded coal particles.
Regards,
Phil Swab Engineering Development Deposition Sciences Inc. Santa Rosa, CA 707-566-3718 phil.swab-at-depsci.com
-----Original Message----- } From: Chris Jeffree [SMTP:cjeffree-at-srv0.bio.ed.ac.uk] Sent: Wednesday, November 24, 1999 3:18 AM To: microscopy-at-Sparc5.Microscopy.Com
Mostly we aim to stain the specimen, rather than the resin, but I have a particular problem where the specimen cannot be stained, but might become visible in negative contrast. Does anyone know of a neat way of staining the RESIN in a TEM section so that it becomes electron dense and provides negative contrast? Alternatively, is there an electron dense resin that can be used for TEM, or is there a way of making a standard epoxy resin mix somewhat more electron-dense than normal?
Chris Jeffree ===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
Years ago, I made up my own doped epoxy by dissolving some Iodaform in the resin before polymerization. After that, I just used the resin like the stock stuff. I don't know but what the brominated stuff might be made with bromoform. I was working from an article in the mid 80s that described using the heavy epoxy to give BSE contrast with coal in the SEM.
Its a fairly simple process, but you want to take precautions around the stuff. It is a little nasty.
Warren S.
At 01:02 PM 11/24/1999 -0500, you wrote:
} Chris, } While I don't do TEM work, I have used several epoxies in other } applications. There are brominated equivalents used with or instead of the } Shell Epon series (for flame retardant purposes) and I have used some from } Great Lakes Chemical, I think they are at 317-497-6100, Lafayette, IN, USA. } Perhaps someone there may be able to suggest a brominated epoxy that will } "drop into" your epoxy protocol (EEW numbers would be a good start) assuming } bromine is heavy enough for your application. } } Dave Audette } OSRAM Sylvania } 71 Cherry Hill Drive } Beverly, MA 01915 } david.audette-at-sylvania.com } } I don't have any interest in Great Lakes Chemical etc. } } } -----Original Message----- } } From: Chris Jeffree [SMTP:cjeffree-at-srv0.bio.ed.ac.uk] } } Sent: Wednesday, November 24, 1999 6:18 AM } } To: microscopy-at-Sparc5.Microscopy.Com } } Subject: Electron dense resins?? } } } } Mostly we aim to stain the specimen, rather than the resin, but I } } have a particular problem where the specimen cannot be stained, } } but might become visible in negative contrast. } } Does anyone know of a neat way of staining the RESIN in a TEM } } section so that it becomes electron dense and provides negative } } contrast? Alternatively, is there an electron dense resin that can } } be used for TEM, or is there a way of making a standard epoxy } } resin mix somewhat more electron-dense than normal? } } } } Chris Jeffree } } ===================================================================== } } DR CHRIS JEFFREE } } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY } } UNIVERSITY OF EDINBURGH
} --- } } I think it worth revisiting this topic from time to time since the } technology and prices seem to change fairly often in this } very competitive market. } } For what it is worth, I just bought a Hewlett Packard } 970Cxi ($399), their latest business-class inkjet printer } and it does a very nice job on high quality paper--better } than the Epson's IMHO. ...
I have no doubts as to your perceptions as to quality, ... indeed I have an HP too and I'm happy with the quality. However, something I realized soon after, when I started investigating archive quality paper and inks (e.g., fade resistent } 50y), is that the ONLY desktop printers supported are the Epsons ... and apparently that hasn't changed. (... hoping someone will let me know differently ...)
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I have seen $199 epson printers that I needed a glass to tell from real photos. But now I need a glass to read the fine print on the medicine bottle:{
} From what I know of color chemistry from photography in the printing industry the only thing remotely perminate is Cibacrome and Kodacrome. I am sure there are some other crome proscess that are the equal. Unless there is somingting i am unaware of there is nothing in the color printer that comes close to being archival. A quick test is to make a print and tape it to a south facing window and compair to a fresh print in two weeks. If you can't tell the differnce it is worth further investigation.
Form my own experinace of about 50 years of photgraphs even Kodacrome has problems. looking at my grandmothers negitives and prints from 100 years ago they are as good as can be done with the same materials today.
The only thing I would trust to be truly archival are seleinum toned black and white seperation negitives. Digital CDROMs that were copied every few years would be my second choice. Digital methods can be archival but the method for storage, test, review and remastering are still in the developmental stages. The principle is simple the exicution complex and subject to human error.
The cheapest safest method I would consider right now is to use a slide printer to print seperations to afga 25, Tmax 100 or tech pan listed in the order of their ease of use and inversly in the order ot their resolution. Litho film could be used as well but it is even touchier to use but most accurate in its depection of the results you would see in a conventionaly printed journal. It would take some tinkering with the exposures to get it right. I would also use fresh mixed developer for every batch. That is what makes agfa 25 and Rodinal almost fail safe. Any reasonably careful person that can follow instructions can make this work after the method has been worked out. It is about the same material my grand mother used except the film is about 30 years newer.
Practiacly I think 2 CD ROMs other than the working copies stored in seprate places as the safest digital method. The technolodgy will develop for arciving them and storing them in two seperat places will out wit the biggest danger to archives, fire.
Back ups are neither easy or convenient. Overtime I have left them to some one else I have been disappointed. In fact I made a living one year reconstructing oil field reports from trashed disk. Fortunately we aren't using Radio Shack Model 3 and Scripsit to store data any more:)
Good luck Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger
} } I think it worth revisiting this topic from time to time since the } } technology and prices seem to change fairly often in this } } very competitive market. } } } } For what it is worth, I just bought a Hewlett Packard } } 970Cxi ($399), their latest business-class inkjet printer } } and it does a very nice job on high quality paper--better } } than the Epson's IMHO. ... } } I have no doubts as to your perceptions as to quality, ... } indeed I have an HP too and I'm happy with the quality. However, } something I realized soon after, when I started investigating } archive quality paper and inks (e.g., fade resistent } 50y), is } that the ONLY desktop printers supported are the Epsons ... and } apparently that hasn't changed. (... hoping someone will let me } know differently ...) } } cheerios, shAf }
Is the need to protect you from bathing in the stuff or accidental spills. I know people that have handled a good part of the stuff bare handed for years with no ill result. It makes sense to wear gloves and rub you hands with a barriar cream with the exeption of Osmium Tetroxide, Cacodylate and Uranyl Acetate that I don't know about the rest are fairly safe if you wash them off quickly. and I have comsumed a liter of ethanol diluted 4 to 6 with water and outher impurities every month for years. You can develop a alergy to epoxy but I would be more worried about breating it than getting it on my hands.
I am not saying you don't need to be as safe as you can but I am annoyed by the safety Nazi's that put Ethanol and Osmium Tetroxide in the same grouping.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger
} } } Can someone please help me determine, or direct me to a comprehensive guide } } for determining, the appropriate gloves for handling each of the following } } chemicals? } } } } Epoxy Resin } } Propylene Oxide } } Glutaradehyde } } Formaldehyde } } Acetone } } Osmium Tetroxide } } Ethanol } } Cacodylate } } Uranyl Acetate } } Lead Citrate } } } }
Thre reference below may be of interest to yourself and other readers. The use of triphenybismuth seems to be of more general interest: from what I have been able to find out it is of extremely low toxicity, as electron-dense materials go.
The "main" author is J.Smid of Syracuse, NY, who makes a speciality of putting bismuth in things, even as a monomer co-polymerizable with styrene and acrylates. If someone has the interest, a suitable compound of bismuth (the heaviest non-radioactive element, and less toxic generally than lead) might be useable in micro applications to replace uranyl acetate.
*} TI: RADIOPAQUE EPOXY-RESINS AU: CHATTERJEE_G, CABASSO_I, SMID_J NA: SUNY SYRACUSE,COLL ENVIRONM SCI & FORESTRY,FAC CHEM,POLYMER RES INST,SYRACUSE,NY,13210 JN: JOURNAL OF APPLIED POLYMER SCIENCE, 1995, Vol.55, No.6, pp.851-856 IS: 0021-8995 DT: Article AB: Transparent, X-ray contrast (radiopaque) epoxy resins were obtained by dissolving up to 25 wt % triphenylbismuth in the commercial epoxy resin prepolymers EPON-815, DER-330, DER-383, and DEN-431 which were then hardened with diethylenetriamine. The radiopacities of the...
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
I posted a comment on the permanence of Epson prints during the summer. To summarise - the prints are decidedly fugitive, even on the expensive glossy papers, and will fade noticeably *even in subdued light* within six months to a year. They are probably not much good for binding into a PhD thesis, for example. Lyson are marketing long-life inks and archival media for Epson printers which may help solve this problem. Check them out on
http://www.marrutt.com
However, Gordon's point is well taken. Cibachrome is probably the most permanent colour print medium currently available. Chris Jeffree } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have seen $199 epson printers that I needed a glass to } tell from real photos. But now I need a glass to read the } fine print on the medicine bottle:{ } } } From what I know of color chemistry from photography in the } printing industry the only thing remotely perminate is Cibacrome } and Kodacrome. I am sure there are some other crome proscess } that are the equal. Unless there is somingting i am unaware of } there is nothing in the color printer that comes close to being } archival. A quick test is to make a print and tape it to a south } facing window and compair to a fresh print in two weeks. If you } can't tell the differnce it is worth further investigation.
===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
The Microscopy Center at Lehigh University wishes to hire a person who has computing and electronic skills to work with electron Microscopes. Training on the electron microscopes will be given if needed. The formal job description may be found on our website at www.lehigh.edu/'inhro/jobs.html. We are looking for a person with general computer skills (software and hardware familiarity with Windows system and Macs) and preferably with some electronics skills (general maintenance and repair of power supplies and other circuits). The person appointed will work with two other staff members to maintain and operate the electron microscopes of the center; will train students in the use of the microscopes and other equipment; will assist the Department of Materials Science and Engineering in it computer needs; will develop remote control systems for the electronic microscopes and, in general will handle a variety of related problems. Lehigh University offers a generous benefit package and competitive salary. Send resume and letter of interest along with salary requirements and references to Jennifer Mohney, Human Resources, 428 Brodhead Avenue, Bethlehem, Pa 18015 EOE/AA Jennifer Mohney HR Associate: Employment
} I have seen $199 epson printers that I needed a glass to } tell from real photos. But now I need a glass to read the } fine print on the medicine bottle:{ } } } From what I know of color chemistry from photography in the } printing industry the only thing remotely perminate is Cibacrome } and Kodacrome. I am sure there are some other crome proscess } that are the equal. Unless there is somingting i am unaware of } there is nothing in the color printer that comes close to being } archival. A quick test is to make a print and tape it to a south } facing window and compair to a fresh print in two weeks. If you } can't tell the differnce it is worth further investigation.
[snip]
Printing digital images can be done in a number of ways. We know about laser printer, ink jets and so forth. If the image is b/w grey scale, then a laser printer is a good choice. There are better but more costly alternatives.
If the image is color, then I think that the best (quality, longevity) is the dye sublimation printer and the type made by Kodak with the Xtralife media. The following URL discusses image fading with other types of media and shows how the Kodak media does not fade, or at least greatly resists fading.
For my color output, I use the Type 1.5 media, Xtralife 3 color, 8-1/2" x 12". This allows a full 8x10 print to be made. The print is photographic quality. Considering the cost of media and ribbon, each printed page costs about $1.95. If I don't care about longevity or just want a good color print for reference, I use the HP Color Laserjet 4500 in Postscript mode. It is 600dpi and does a very good job. Cost per page is about 25 cents or less, depending on color content and type of paper used.
The difference between photographic quality dye sublimation prints and those from Cibachrome/Ilfochrome is that these chrome prints have accentuated contrast and higher color saturation. The dye sublimation prints are more realistic and have higher fidelity to the original image file. The other issue is that the only way I know of obtaining chrome prints is make a master E-6 slide, since the chrome is a direct positive from a positive color slide.
Paul J. Gerroir wrote: ================================================= To All, I am interested in hearing from those of you who can offer me some suggestions on the sectioning of papers, specifically coated papers or those papers upon which wet (ink-jet inks) or dry (toner) forms of printing have been completed.
Typically we overcoat the paper surface, i.e. sputter a film of metal then embed the paper sample in epoxy. Afterwards we have prepared thick sections (0.5 - 0.8 microns) without water in the boat using either a glass or diamond knife followed by examination using a light microscope. To examine these samples using the TEM requires thinner and non-distorted sections. Obviously sectioning with water in the boat swells the paper, possibly interfering with the coating on the paper.
Anyone having experience in this area or that can offer suggestions I would appreciate hearing from. ======================================================== We have been doing these kinds of samples in the analytical services side of our business since the mid-1970's. The exact approach might be tempered by the nature of the substrate paper, presence and amount of clay coating, presence and amount of resin binders, and also the type of ink. The surface definitely has to be passivated, otherwise the ink and binder can dissolve off in the embedding resin. We use only diamond knife ultramicrotomy, for the passivation layer, Pt is sometimes better than gold because of a lower tendency to be smeared by the knife. If the dense layer from the metal interferes with surface observations of ink pigment, we switch to an Al coating by vacuum evaporation.
We use our own SPI-Pon™ 812 resin but some of the other "Epon® substitute" resin systems probably would work just as well. We have never been able to do these with glass knives, so only diamond knives (SPI Materials Science) are used. These are 45° knives. Obviously other knives would work but most other "materials science" knives tend to come standard with 55° angles. A more "blunt" edge on these kinds of samples generally produces too much compression, or at least that has been our experience. The sections are done cryo and picked up "dry". The only drawback of this approach is that the sections tend to be a bit thicker than if done at RT but there is a marked reduction in artifacts. There tends to be more than enough contrast present so the reduced contrast due to the thicker sections tend not to be too much of a problem.
If the paper substrate is especially porous, then a vacuum embedding is needed but we don't do that unless it turns out to be necessary.
Disclaimer: Structure Probe, Inc., the laboratory services part of our firm , does this kind of work on a purchase order basis for clients as a laboratory service. SPI Supplies offers the embedding resins and diamond knives mentioned in this posting.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Does anywone know of a reference showing apoptotic epithelial cells? I'm looking for same in some tissues but need to be sure of the morphology to expect.
} } If the image is color, then I think that the best (quality, longevity) is } the dye sublimation printer and the type made by Kodak with the } Xtralife media. The following URL discusses image fading with } other types of media and shows how the Kodak media does not fade, } or at least greatly resists fading. } } http://www.kodak.com/global/en/professional/products/printers/dyeSub/fading .shtml } } For my color output, I use the Type 1.5 media, Xtralife 3 color, 8-1/2" x 12". } This allows a full 8x10 print to be made. The print is photographic quality. } Considering the cost of media and ribbon, each printed page costs about $1.95. } If I don't care about longevity or just want a good color print for reference, I use } the HP Color Laserjet 4500 in Postscript mode. It is 600dpi and does a very } good job. Cost per page is about 25 cents or less, depending on color content } and type of paper used. } } The difference between photographic quality dye sublimation prints and those } from Cibachrome/Ilfochrome is that these chrome prints have accentuated } contrast and higher color saturation. The dye sublimation prints are more } realistic and have higher fidelity to the original image file. The other issue } is that the only way I know of obtaining chrome prints is make a master } E-6 slide, since the chrome is a direct positive from a positive color slide.
The obvious fading in the red half the image shows dye sub has serious fading problems. I seriously doubt that a laminate would extend the life by a factor of more than ten. Johnsons past wax will double a color prints fade life just protecting if form UV and polutants.
Cromes are notoriously red. It a appears that the Kodak laminate does improve the life of dye sub prints. I doubt that approach archival quality of silver based color separation on film in life or cost. Most of us have a slide printer around and with a little tinkering it can be made to produce color separations on B&W film that we know will last over 100 years if properly processed. And you can do it in the sink in the coffee room if you have a changing bag. With the cost of 35 mm film from $10 cents a image and a years suppose for developer fixer, and fixer clearing agent and photo flow for 30 bucks.
Admittedly it is a little trouble to scan the negatives and make the image in photo shop and print them again. But If you want good images 50 years from now that is the cheapest safest way of doing it.
Now the question of do we need archival images is another question. I seldom use old data. But for some work it is important.
A properly designed digital back up system wiht multiple copies and periodic remastering should be it's equal but it is an active system and the former is a passive system.
If all you need is a year or two the dye sub with a lamination stored in the dark will probably do but If you want 50 year storage I seriously doubt it will make the trip.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger
I have not actually done this as you suggest, but Ruthenium Tetroxide stains many polymers, and I have seen it provide some staining contrast in epoxies ( I usually use Spurr Low Viscosity). You might initially try a rigorous (RuO4 0.5%) vapor staining of the sections, and see if it will work. The RuO4 (unlike OsO4) does not need a lot of unsaturation in the polymer to get some staining started. Good Luck, Brad
} ---------- } From: Chris Jeffree[SMTP:cjeffree-at-srv0.bio.ed.ac.uk] } Reply To: c.jeffree-at-ed.ac.uk } Sent: Wednesday, November 24, 1999 5:17 AM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Electron dense resins?? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Mostly we aim to stain the specimen, rather than the resin, but I } have a particular problem where the specimen cannot be stained, } but might become visible in negative contrast. } Does anyone know of a neat way of staining the RESIN in a TEM } section so that it becomes electron dense and provides negative } contrast? Alternatively, is there an electron dense resin that can } be used for TEM, or is there a way of making a standard epoxy } resin mix somewhat more electron-dense than normal? } } Chris Jeffree } ===================================================================== } DR CHRIS JEFFREE } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY } UNIVERSITY OF EDINBURGH } Daniel Rutherford Building } King's Buildings, Mayfield Road } EDINBURGH, EH9 3JH, Scotland, UK } Tel. #44 131 650 5345 } FAX. #44 131 650 6563 } Mobile 0410 585 401 } email c.jeffree-at-ed.ac.uk } SEM / TEM bookings sem-at-ed.ac.uk } ===================================================================== }
After side by side demo's of Nikon and Olympus inverted microscopes, I have decided to buy 2 Olympus inverted scopes for a variety of reasons (including Olympus made me a fantastic deal on cost). One scope with be for my new Biorad Radiance 2000 and the other for a deconvolution set up (Isn't life tough?) .
I intend to buy 10 objectives including three 60x objectives (LWD Plan FLuorite NA 0.7, Plan Apo Oil NA 1.4, and a Plan Apo water NA 1.2). Olympus makes a "special" Plan Apo Water Immersion with the same NA but with a "guaranteed" PSF. My rep is somewhat vague on this point but apparently this is one that has gone through an extra round of quality control and has a better PSF. It costs several thousand more. I plan to demo them both but was wondering if any Olympus users of either or both of these objectives wanted to comment on or off the public listserver. Thanks, Tom Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
----- Original Message ----- } From: "Brian Gortney" {gortn-at-earthlink.net} To: {Microscopy-at-MSA.Microscopy.Com} Sent: Saturday, November 27, 1999 8:09 PM
------------------------------------------------------------- PHILADELPHIA SOCIETY FOR MICROSCOPY
AN AFFILIATE OF THE MICROSCOPY SOCIETY OF AMERICA and THE MICROBEAM ANALYSIS SOCIETY ------------------------------------------------------------
MEETING NOTICE: WEDNESDAY DECEMBER 1, 1999
FORENSIC MICROSCOPY: EVIDENCE OF MICROSCOPICAL SIZE AS AN AID IN ESTABLISHING ASSOCIATION.
Thomas A. Kubic, MS, JD, FABC
--- SPONSOR--------- TED PELLA, We suggest that you look at the Ted Pella, Inc. web site (www.tedpella.com) to preview hundreds of new products not in the present catalog.
-----Meeting Details----- Location: LRSM Building (Laboratory for Research on the Structure of Matter), UPENN, 33rd and Walnut (see map). Parking available behind LRSM after 5:00 PM.
Schedule: 5:30 Social hour, 6:30 Dinner, 7:15 Talk
Cost of Dinner: Members $12.00, Non-Members $15. Students $6,
Menu: ITALY AT A GLANCE: MESCULIN MIXED GREENS, SPINACH PASTA, CHICKEN BREAST SHRIMP CARBONARA, HOMEMADE FOCACCIA AND TUSCAN BREAD LAYERED MOCHA CREAM TORTE, COFFEE SERVICE
-----Reservations----- Reservations required. I'll order a couple extra meals, but if you don't make a reservation, you might not get dinner!
By E-Mail (preferred): Send your name and affiliation to PSM-RESERVATIONS-at-INAME.COM By Phone: Call John Reffner, 215-619-5283 DEADLINE MONDAY, Nov 29, 4:00 pm.
-----Abstract-----
Crime investigators have long relied on crime scene investigators and laboratory scientists to aid in solving cases by establishing association(s) between suspects, victims, and crime scenes. Often the physical evidence that is recovered and analyzed is microscopical in size and requires the skills of a competent microscopist to reveal the critical information. Light microscopes, particularly the Polarized Light Microscope and the infrared microspectrophotometer, are the most useful of the photon techniques, while the Scanning Electron Microscope, especially when mated to an Energy Dispersive X-ray Spectrometer is the electron beam method of choice in the crime laboratory.
Our discussion will begin with the role of the criminalist, concentrating on the forensic microscopist and the methods, techniques, and the instruments employed for the examination of certain trace evidence types. A number of cases will be discussed as examples of how microscopical examination aided in associations(s). Most examples will include the concurrent application of more than one method in the analysis.
The talk will conclude with a number of examples of how the recently developed variable pressure SEMs are an aid to the analysis of samples of forensic import.
-----Biography-----
Mr. Kubic is a chemist and microscopist by training and a forensic scientist and criminalist by avocation. He received his BA in chemistry from C. W. Post Collage, MS in Chemistry from Long Island University and Juris Doctor from St. John's University. He is currently a Ph. D. candidate in forensic science at City University of New York. Mr. Kubic studied microscopy under Drs. John A. Reffner, Walter McCrone, Peter DeForest, and Tom Emma Mr. Kubic completed 23 years as a criminalist in a crime laboratory prior to his retirement in 1995, and joining AMRAY as a consultant in forensic scanning electron microscopy. He has been the laboratory director of a New York State accredited environmental laboratory and a NIST accredited asbestos laboratory.
Mr. Kubic is currently on the faculty of John Jay College, where he instructs in electron microscopy, forensic chemical instrumentation and scientific testimony. He continues to serve as a technical expert with NIST in it's NVLAP accreditation program of asbestos laboratories.
Mr. Kubic continues to actively research the analysis of particulate materials by various light, microspectrophotometric, and electron microscopical methods. He is especially interested in the evaluation of trace evidence to establish associations between persons, places, and things.
-----IMPORTANT NOTICE: ELECTIONS----- Elections for all positions on the executive council will be held at the upcoming meeting. Several people have come forward and expressed an interest.:
President: Pat Connelly (note that we do not have a president elect for 1999 to fill the role for 2000.)
President Elect: Robert Carlton
Secretary - Treasurer: John Reffner (to continue from 1999)
ANY OTHERS????
The floor will be open for nominations at the meeting. In the absence of anyone actually running against anyone else, I've made the executive decision as newsletter editor* not to bother making up ballots to be mailed in. IF YOU HAVE ANY QUESTIONS OR CONCERNS, PLEASE LET ME KNOW AND I WILL DO MY BEST TO ADDRESS THEM. --------------------------------------------------------------------------
FOR MORE INFORMATION PLEASE CONTACT:
Philadelphia Society for Microscopy Executive Council, 1999
Past President Charles Michel (302) 695-3881 Secretary-Treasurer John Reffner (215) 619-5283 Corporate Liaison Thomas Hoffman (609) 859-2434 --------------------------------------------------------------------------
This notice is mainly aimed at Australian colleagues.
I am disposing of a TN-5500 X-ray Analyser with LSI11/73 microcomputer, floppy diskette drive (8") , winchester drive, high resolution 13 inch colour monitor, high quality dot matrix 120 cps printer/plotter; complete with instruction manuals, software and master diskettes (8").
It is yours for free if you can make arrangements to have the unit taken away. Hans G Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science Electron Microscopy Hawthorn, 3122, Melbourne - Australia
Pre-war the company Zeiss was located in Jena and with Leitz they shared the reputation as the World's best microscope manufacturers. After the war senior staff established Zeiss Oberkochen in West Germany. Jena was located in East Germany and was run as a state enterprize. In time, Zeiss Oberkochen became the better manufacturer of the two Zeiss Companies. Eventually the two companies combined. "Aus" means "from" or "out"; Jena (spelled with one n) is a small city about 70km SW from Leipzig and "next door" to historical Weimar. (Carl) Zeiss clearly is the successor company for Jena. Who knows how long for parts are kept? At the time, microscopes from Jena were very good, but not in the very top league. Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Monday, November 29, 1999 8:49 AM, Brian Gortney [SMTP:gortn-at-earthlink.net] wrote: } } } ----- Original Message ----- } } From: "Brian Gortney" {gortn-at-earthlink.net} } To: {Microscopy-at-MSA.Microscopy.Com} } Sent: Saturday, November 27, 1999 8:09 PM } Subject: LM Aus-Jenna Labroval } } } } Hello: } } I am contemplating the purchase of a used Aus-Jenna Labroval light } } microscope for us in biological work it is approximately 20 years old } } however is in excellent condition.I am hoping some of you are familiar } with } } this unit as I have a number of questions: } } 1.) Where are they made ? } } 2.) Is this a good quality unit? } } 3.) I was told that Aus-Jenna merged with Zeiss,does anyone know for sure? } } 4.) Are parts still available? From where? } } 5.) are the Aus optics comparable with zeiss or Leica ? } } Thank you all for your time. } } Brian W.Gortney } } } } }
We have had good luck with our Linkham stage. It is very stable, and h= as a temperature range from -100 C to 600 C. It also has options to overlay=
temperature data onto a video signal. However it is fairly expensive.
Joe Neilly Abbott Laboratories D-45M, AP31 200 Abbott Park Rd. Abbott Park, IL 60064-6202 voice: (847)-938-5024 fax: (847)-938-5027 e-mail: joe.neilly-at-abbott.com
} We are interested in recommendations for a hotstage for a light micros= cope. } If there is cooling capabilities that will be an added plus, if it doe= sn't } compromise the heating function. Our Mettler FP5 finally failed after } faithfully servicing us for 25 years. We have looked at the Mettler FP= 82, } seen the Linkam and heard about an Instec (HCS600). Any experience wit= h } these would be appreciated. } Vendors are welcome to contact me directly at: } GaroneL-at-Polaroid.com } Thanks, } Lynne Garone } Polaroid Corp. =
***************************************************************************** } } } } Dear All } } } } I am increasingly being asked to prepare bacterial suspension } } cultures (E. coli mutants, mostly) for TEM sections. } } Not being a bacterial person (I prefer my organisms to come in } } lumps that need to be cut up) I would be grateful for advice on the } } preferred method of processing bacterial cells, particularly in } } suspension cultures, but I would also be interested in tips for } } dealing with individual colonies on agar. } } } } Thanks in advance } } Chris Jeffree *******************************************************************************
Brian McIntyre mailto:mcintyre-at-optics.rochester.edu Sr. Engineer lab: 716-275-3058/4875 River Campus EMLab fax: 716-244-4936 University of Rochester Rochester, NY 14620
"The most important thing a father can do for his children is to love their mother." - Unknown
The information in other postings is correct: Jena was the original home of what we now know as Zeiss and that the two facilities diverged for a period of time after the second world war, with most of the power going to the "new" Zeiss facility in Oberkocken. There is lots of interesting history in that whole situation, including a battle in the World Court for who was going to be allowed to use the Zeiss name and trade mark. The bottom line: the two have now reunited under the regular Zeiss trademark.
Now, for the instrument under discussion. In the early 80's I was privileged to be one of the national training resources for some of the Jena line (especially the Interphako and Amplival Pol). I found the instruments from this time period to be very well constructed with good quality optics. Regarding availability of parts, I would check with Zeiss USA (located in Thornwood, NY: 914-747-1800). There are also second-hand sources: from the list below, Martin Instruments has had the most experience with Jena gear.
MicroOptical Methods Dennis O'Leary Albany, NY 518-482-8200 Spectra Services Mike Specht Rochester, NY 716-654-9500 Vermont Optechs John Oren 802-425-2040 Martin Instruments Bob Martin Easley, SC 864-859-2688
Good luck... and if you buy this Jena scope, let me know how you like it.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 05:49 PM 11/28/99 -0500, Brian Gortney wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi All, We have a jet freezer in need of repairs, and are having difficulty locating a company/person to do it. Has anyone had any experience with this or any advice? Thanks for your help, Kristen Kristen A. Lennon Cell, Molecular & Developmental Biology Group Department of Botany & Plant Sciences University of California Riverside, CA 92521 kalen-at-citrus.ucr.edu
A colleague asked me about an EM course that would teach him the basics of electron microscopy with focus on thin sectioning and TEM operation. There used to be such a course here until they closed our EM facility last year and retired the woman who ran it. I know about the Lehigh courses but I am looking for something closer and more basic in California. Any suggestions would be appreciated.
Hi, I have an old AO/Reichert Ultracut ultramicrotome that needs service. Does anyone know a service person in the southeast? The Leica service person has stood me up twice :-( on scheduled appointments) so I'd love to hear your opinions/thoughts on other companies/service people in the area. thanks, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
Still waiting for word from our Site IH for the info on permeation data for nitrile gloves. She is on vacation this week, but I am assured that she has some data on these gloves. Apparently, Best Manufacturing ( in Georgia) has had or has done some permeation testing on their N-dex line of gloves. This is the manufacturer that we use. They can be found at http://www.bestglove.com/ I believe Fisher caries their products, and I am told that a higher dexterity Viton and Butyl glove is forthcoming from Best. You can download a limited chemical resistance guide (CHEMREST) from Best's web site. I use the N-dex, 6 mil, powder free 9005 gloves as a general protection when there is no risk of a severe splash/submersion/contamination, and, as I mentioned previously - when using some of the nastier materials we make frequent checks and glove changes while having double glove protection.
There is presently a draft standard (ANSI/ ISEA 105 1999) on glove - definitions, testing, and classification of protection that will probably make glove comparison and selection a little easier in the future, but until it is adopted - I guess its every hand for itself.
Good Luck, Brad Huggins BPAmoco, Naperville, IL hugginbj-at-bp.com
} ---------- } From: Caroline Schooley[SMTP:schooley-at-mcn.org] } Sent: Wednesday, November 24, 1999 5:00 PM } To: Huggins, Bradley J } Subject: RE: Protective Gloves } } } Yes, I have seen permeability data on the N-DEX gloves, but do not have } it } } handy. I'll forward any references or tables ASAP. } } } } Brad Huggins } } Thanks! There's no deadline on this; at your convenience. Will you } please } cc to the listserver? } } Caroline } } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html } Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html } }
Feathery spots have formed on the blue (writing) surface of silver-colored CD-Rs from a single manufacturer (name withheld for now). Some spots were present fresh from the jewel cases; spots have formed on others since the CDs were burned.
Your reports of similar observations and causes will be appreciated. Please reply on or off list, as you prefer.
Important dates =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
02 June 2000 - deadline for submission of contributed papers 30 June 2000 - notification of acceptance of papers 28 July 2000 - deadline for registration with reduced fee and submission of accepted papers in their final form 11 October 2000 - SGI'2000 Conference
Main topics of the Conference =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D
The Conference will be a forum for SGI users, independent software vendors, applications developers, research and industry scientists, as well as for decision makers.
The following topics of high performance computing and visualization on SGI platforms will be covered by the Conference: - experience with SGI products for high performance computing, - large scale simulations, - applications in science, engineering and industry, - visualization, graphics and multimedia, - system management, resource management and batch systems, - tools and programming environments, - data storage.=20
The Conference will include=20 - tutorials,=20 - invited talks,=20 - contributed presentations from the SGI users community,=20 - presentations of new SGI products, - round table discussions,=20 - social events.
We invite participants with experience in computing on SGI systems to submit papers for SGI'2000. A reduction of the registration fee will be offered to one of the authors of each accepted contributed paper.
Papers, 6 to 10 A4 pages, should be sent via email to the Organizing Committee before June 02, 2000. Papers will be reviewed taking into account originality, significance of presented results, relevance to SGI'2000, accuracy and way of presentation.=20 For a detailed submission procedure see http://www.cyf-kr.edu.pl/sgi2k/.
Accepted papers will be included in the Conference program as oral or poster presentations, and they will be printed in a Proceedings=20 volume which will be distributed to participants at the beginning of the Conference.
It will be also possible to present virtual posters which may accompany an invited lecture, a contributed oral presentation as well as a classical poster.
All participants who intend to present virtual posters are requested to prepare posters at their own WWW sites and email http address, author(s) name and title of a poster to the Organizing Committee not later than September 30, 2000. Then, links will be done from the Conference WWW page to virtual posters.
Beth, I was due for pm on my Ultracuts in March. Leica service didn't show up until August. I didn't get copies of my service reports until yesterday! There have been some serious problems with service since Charlie Christensen moved over to confocal. Try calling Dan Simkowski at 1-800-248-0123 or his secretary, Rita at 847-405-8130. That's how I was able to get my service reports. If you deal with Vashaw Scientific, give them a call at 770-447-5632 to complain. They are the Leica people here in Atlanta. Good Luck, Bob Santoianni Emory University Hospital Atlanta, GA
I am wondering if this is the infamous 'CD Rot' condition that was prophesied at the dawn of the CD era.
Ed Sharpe archivist for SMECC
} Microscopy-at-Sparc5.Microscopy.Com (Microscopy-at-Sparc5.Microscopy.Com) } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Feathery spots have formed on the blue (writing) surface of silver-colored } CD-Rs from a single manufacturer (name withheld for now). Some spots were } present fresh from the jewel cases; spots have formed on others since the } CDs were burned. } } Your reports of similar observations and causes will be appreciated. } Please reply on or off list, as you prefer. } } James }
All Jet Freezers manufactured by RMC are now serviced by Ventana Medical Systems, Inc. Tucson, AZ.
Please call 800-227-2155, listen for the Customer Service prompt. The TCC center is manned 24/7.
Alternatively you may visit our website at www.Ventanamed.com and select Support.
Steve Miller North America Sales Manager Microscopy Products Group Ventana Medical Systems, Inc. 3865 N. Business Center Dr. Tucson, AZ 85705 Direct Phone: 520-690-2753
Good Morning, Jo Ann, There is an excellent program for Electron Microscopy at San Joaquin Delta College in Stockton, CA. You should really look into it. The address is: San Joaquin Delta College 5151 Pacific Avenue Stockton, CA 95207 http://www.deltacollege.org Click on Depts, then Electron Microscopy.
They offer individual classes and a very good, highly recommended two year program! Dr. Judy Murphy has an infinite wealth of knowledge!! Sincerely, Jo Dee Fish
JoAnn Buchanan wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } A colleague asked me about an EM course that would teach him the basics of } electron microscopy with focus on thin sectioning and TEM operation. There } used to be such a course here until they closed our EM facility last year } and retired the woman who ran it. I know about the Lehigh courses but I am } looking for something closer and more basic in California. Any suggestions } would be appreciated.
-- Jo Dee Fish Electron Microscopy Assistant The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 858-646-3100 ext.3620
We are presently in the market for an inverted microscope confocal with dual-photon capability. We've narrowed it down to the Leica TCS SP (the NT plus spectrophotometer head) and the Zeiss LSM510. If any happy (or indeed unhappy) owners would like to give me some feedback I'd be something close to eternally grateful. Thanks in Advance
Barry Shaw Barry Shaw Senior Imaging Technician Molecular & Cell Biology E Floor School Of Biomedical Sciences University of Nottingham Medical School NG7 2UH
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi, I was just given some VERY small roots to be fixed and embedded for ICC. Fixation is not a problem but I am not sure I can keep track of these very tiny root pieces once they are infiltrated and embedded in LR White. I would like to stain them with something that is not soluble in ETOH and will also not interfer with the ICC labeling. Any suggestions? Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
id {XFPBGZF5} ; Tue, 30 Nov 1999 09:27:54 -0800 Message-ID: {F129F546524BD31199C200902798ABD6070563-at-CMEXCHANGE.Stanford.EDU}
This previous job listing had the wrong contact email address. The proper address is pbs-at-stanford.edu
Thank you.
Ruth
*************************************************
Stanford University Palo Alto, California
POSITION: Life Sci Res Asst II (#J992664)
DESCRIPTION: Position is responsible for electron
microscopy in a Neuroscience Lab. Duties to include: ultrathin serial sectioning; serial section analysis using electron microscope; post-embedding immunocytochemistry; photography-developing and processing of prints; stock supplies, keep inventory; develop new techniques: conduct literature searches; test alter and implement new methods to improve lab procedures and maximize productivity; requires results/data interpretation; will provide training to research personnel in photographic and electron microscopic techniques; the individual will assist in the preparation of scientific paper for publication; will independently: prepare tissue for electron micrographic examination, examining tissue, analyzing data, and preparing data for publication. Comply with governmental regulation and University policies regarding health and safety and observe and support health and safety practices including those specifically related to the work environment.
QUALIFICATIONS: Bachelor's degree in a related field is required; master's degree preferred; plus 3 years of applicable experience. Must be able to work effectively in a team and independently. Must exercise considerable judgement, initiative, and resourcefulness. Must be able to lift up to 50 lbs. Fluent in written and verbal English. Basic knowledge in use of personal computers, photographic equipment, and electron and light microscopes.
Salary range: $3404-4544. Exempt. New position. For more information email Dr. Buckmaster at pbs-at-stanford.edu
Send resumes to:
Dr. Paul Buckmaster Department of Comparative Medicine Building 330, Quad 7, RAF-1 Stanford University Stanford, CA 94305
If everything goes according to schedule, I will have a vintage JEOL 100B to offer as surplus to our needs. This baby is as clean as they come, low miles and plenty of spare parts.
Seriously, I have to go through our surplus office, but they usually ask me if I know of anyone who might be interested. If you want to know more, drop me a line and I will be happy to fill you in on the details. I don't really expect to get much for this heap, seeing it go to a good home would be enough for me (don't know about the bean counters in surplus).
Jonathan Krupp Microscopy & Imaging Lab University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
I have always supposed that bone was too hard to thin section with a diamond knife and would damage the knife, but is this true? Has anyone tried to thin section bone by accident or on purpose, and what was the result? How about a thin lamina of bone within the block?....Thanks..Tom Reese
They do all our microtomes, including the Sorvall.
On Mon, 29 Nov 1999, Beth Richardson wrote:
} Date: Mon, 29 Nov 1999 17:05:17 -0500 } From: Beth Richardson {beth-at-dogwood.botany.uga.edu} } To: microscopy-at-sparc5.microscopy.com } Subject: waiting at the microtome } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, } I have an old AO/Reichert Ultracut ultramicrotome that needs service. Does } anyone know a service person in the southeast? The Leica service person has } stood me up twice :-( on scheduled appointments) so I'd love to hear your } opinions/thoughts on other companies/service people in the area. } thanks, } Beth } } ************************************** } Beth Richardson } EM Lab Coordinator } Botany Department } University of Georgia } Athens, GA 30602 } } Phone - (706) 542-1790 } FAX - (706) 542-1805 } Email - beth-at-dogwood.botany.uga.edu } ************************************** } } } }
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
Hello everyone! Please respond directly to me at the e-dress below.
We have an opportunity to buy a schottky emmitter from FEI directy and would appreciate any info on the track record of these un-warranteed/guaranteed products direct from them ASAP. Thanks in advance
John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602-2403 (706)542-4080 jpshield-at-arches.uga.edu
} Hi, } I was just given some VERY small roots to be fixed and embedded for } ICC. Fixation is not a problem but I am not sure I can keep track of these } very tiny root pieces once they are infiltrated and embedded in LR White. } I would like to stain them with something that is not soluble in ETOH } and will also not interfer with the ICC labeling. } Any suggestions? } Debby } } Debby Sherman, Manager Phone: 765-494-6666 } Microscopy Center in Agriculture FAX: 765-494-5896 } Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu } Purdue University } 1057 Whistler Building } West Lafayette, IN 47907-1057 ******************* You can try eosin. We've used it to impart some color to early chicken embryos that were later labelled by immuno-fluoresent markers. These were embedded im paraffin, and viewed in the LM, but it may cross-over.
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Confocal Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
Greetings, Debby Sherman asked about handling small bits of root. I make=20 my living handling these snippets so perhaps I can help.
As far as stains go, we have used two. We have made a=20 saturated solution of Fast Green in ethanol. We make an 8% solution=20 of fast green in 100% ethanol and then add 1-2 =B5l to each half ml of=20 volume with sample. I have also used a 0.15 % aqueous solution of=20 basic fuscin. In this case i collect the samples in that prior to=20 dehydration, and they stay nicely purple throughout.
The other thing that I do to greatly simplify my life is to=20 put each root on a wire loop, encased in Formvar. Here is an except=20 from a lab protocol I have:
We get best results with a Formvar loop method. In this=20 method, a loop of copper wire (36 gauge) is made and flattened=20 between two flat pieces of steel. Then small rectangles of 0.25%=20 =46ormvar in ethylene chloride are floated on water and the loop=20 plunged into the middle of the rectangle so that a film of Formvar=20 surrounds the wire loop. A number of loops are made in advance (can=20 be days ahead). A root is then placed on the Formvar surface, the=20 excess cut away with a razor and then this assembly is coated with=20 another Formvar layer, in the same was as above, thus encasing the=20 root between Formvar. This procedure provides better flat embedding=20 than agarose. Up to three loops can be put in a single vial. Note:=20 one does not need super "EM" grade Formvar films, so this is not a=20 hassle at all.
When its time to embed, just cut off the stem of the loop and=20 embed the loop with the sample on. The loop is heavy enough to sink=20 and keep the root nice and flat. The copper is so thin you can cut=20 through it with a razor in the block. Or if you like, you can=20 excavate it from the block and pull it out.
Alternatively, if you don't want to mess with loops and=20 =46ormvar, you can use agarose. Excise a 3mm tip segment and encase it=20 within a small droplet of 2% low gelling-temperature agarose (Type=20 VII from Sigma). When the agarose sets, the root-containing drop is=20 transferred to 10% ethanol. We get better results with Formvar but=20 the agarose will work.
The point of the loops or the agarose is to facilitate=20 exchange of solutions without loss of samples. It is a snap to suck=20 out the old solution and put in the new with your samples in agarose=20 pellets or held onto loops. With the agarose, you can keep all of the=20 samples of a single treatment in one vial (this saves time and=20 solution) but with the formvar loops, it is hard to use more than=20 three loops per vial.
I hope this helps. Good luck, Tobias Baskin } } Hi, } I was just given some VERY small roots to be fixed and embedded for } ICC. Fixation is not a problem but I am not sure I can keep track of these } very tiny root pieces once they are infiltrated and embedded in LR White. } I would like to stain them with something that is not soluble in ETOH } and will also not interfer with the ICC labeling. } Any suggestions? } Debby } } Debby Sherman, Manager Phone: 765-494-6666 } Microscopy Center in Agriculture FAX: 765-494-5896 } Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu } Purdue University =20 } 1057 Whistler Building } West Lafayette, IN 47907-1057 _ ____ ^ __ ____ Tobias I. Baskin / \ / / \ / \ \ University of Missouri / | / / \ \ \ Biological Sciences /___/ /__ /___ \ \ \__ 109 Tucker Hall / / / \ \ \ Columbia, MO 65211-7400 USA / / / \ \ \ voice: 573-882-0173 / /____ / \ \__/ \____ fax: 573-882-0123
Dear Beth: Our AO/Reichert Ultracut ultramicrotome has been serviced in the past by Helmut Patzig of Microscopical Optical Consulting Inc., You might contact him by phone (914) 268-6450 or by e-mail MOCLeica-at-AOL.com I know he has customers far and wide so I hope Athens, GA would be within his range of coverage. Hope this helps, Henry
Henry Eichelberger, Manager Electron Microscopy Facility Department of Biological Sciences Binghamton University Binghamton, NY 13902-6000
phone: (607) 777-2682 fax: (607) 777-6521 e-mail: heichelb-at-binghamton.edu ******************************************************* Original Message: -------------------------------------------------------- Hi, I have an old AO/Reichert Ultracut ultramicrotome that needs service. Does anyone know a service person in the southeast? The Leica service person has stood me up twice :-( on scheduled appointments) so I'd love to hear your opinions/thoughts on other companies/service people in the area. thanks, Beth
************************************** Beth Richardson EM Lab Coordinator Botany Department University of Georgia Athens, GA 30602
} Call TekNet 1 800 835 6386 } jon-at-teknetinc.com } } They do all our microtomes, including the Sorvall.
I have also had very good service from Tek-Net.
} On Mon, 29 Nov 1999, Beth Richardson wrote: } } } Hi, } } I have an old AO/Reichert Ultracut ultramicrotome that needs service. Does } } anyone know a service person in the southeast? The Leica service person has } } stood me up twice :-( on scheduled appointments) so I'd love to hear your } } opinions/thoughts on other companies/service people in the area. } } thanks, } } Beth
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
} I am wondering if this is the infamous 'CD Rot' condition that was } prophesied at the dawn of the CD era. } } Ed Sharpe archivist for SMECC } } } } } } Feathery spots have formed on the blue (writing) surface of silver-colored } } CD-Rs from a single manufacturer (name withheld for now). Some spots were } } present fresh from the jewel cases; spots have formed on others since the } } CDs were burned. } } } } Your reports of similar observations and causes will be appreciated. } } Please reply on or off list, as you prefer. } } } } James } }
Green and blue CD-R media have been known to be disadvantageous for some time now. The most reliable is the silver. I've had excellent results with Memorex, UPC 0-34707-03131-9, PN 3202-3131, Philips 8945-731-51111, and Sony CDQ-74SZA.
I have not done much testing on CD-RW media as yet.
We will be offering a semester long (15 week) EM class, Biology 5060. It will begin on January 10, 2000. It will cover biological specimen preparation for SEM and TEM, and use of the TEM and SEM. Students will produce a portfolio of digital images from their own original work. Class size is limited to 8, but space is still available. If you are interested in attending, please contact me to make arrangements.
William McManus Supervisor Electron Microscopy Facility Department of Biology Utah State University Logan UT 84322-5305
billEMac-at-cc.usu.edu 435-797-1920
William McManus Supervisor Electron Microscopy Facility Department of Biology Utah State University Logan UT 84322-5305
} } I am wondering if this is the infamous 'CD Rot' condition that was } prophesied at the dawn of the CD era. } } Ed Sharpe archivist for SMECC }
Ed,
OK I'll bite. Tell us more about this 'CD Rot'. I think not leaving them lying around exposed to direct sunlight or even strong daylight for years on end would be a reasonable precaution to take.
(Does anybody know of a suitable fixitive ;)
Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457 Ansto Materials Division Fax: 61-2-9543-7179 PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au Australia www: http://www.ansto.gov.au/
Again some items for free, that is, if you can arrange pick up.
I am disposing of a complete ETEC Autospec WD X-ray Spectrometer, ie. the spectrometer with crystals and separate electronic operational console containing; Detector Control Crystal Select (LiF, PET, LOD, RAP) Spec.Pos.Control Scaler Timer Amplifier -PHA Linear Ratemeter. This WDS was originally attached to our ETEC Autoscan SEM, which (just a point of general interest) is still working satisfactory after 26 years of usage.
Cheers
Hans Brinkies
Hans G Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science Electron Microscopy Hawthorn, 3122, Melbourne - Australia
Thank you for the comments, on-list and off-list, about my query regarding deterioration of CD-Rs, before and after writing files. Inspection today of two disks written in September showed that the same deterioration has occurred on one of the disks, since it was written.
No one has recognized and identified the cause for the deterioration - a few have mentioned "CD rot" - but one respondent did describe a similar problem with disks from the same manufacturer, fresh from the package.
I attempted to contact the manufacturer today, but was unable to get past voice mail. I am still hesitant to disclose the name of the manufacturer, but will state the model code, which anyone who has these disks can recognize:
CDQ-74CN
The respondent who found a similar problem used model code:
CDQ-74BN
Our affected disks are no longer readable, or only partially so. Because the disks are used only as secondary storage (backup), and are copied, we have lost no data or images. Still, this is an inconvenience worth mentioning.
Again, thank you.
James
} Feathery spots have formed on the blue (writing) surface of silver-colored } CD-Rs from a single manufacturer (name withheld for now). Some spots were } present fresh from the jewel cases; spots have formed on others since the } CDs were burned. } } Your reports of similar observations and causes will be appreciated. } Please reply on or off list, as you prefer. } } James } } ------------------------------------------------------------------------- } } James Martin } Director of Analytical Services & Research } Williamstown Art Conservation Center } james_martin.tripod.com/dasr.htm } } Research Scientist in Chemistry } Williams College } james_martin.tripod.com/williams.htm } } *** Please don't send e-mail attachments. Cut-and-paste text into the } body of an e-mail message. *** } } }
Tom Reese wrote: ========================================================== I have always supposed that bone was too hard to thin section with a diamond knife and would damage the knife, but is this true? Has anyone tried to thin section bone by accident or on purpose, and what was the result? How about a thin lamina of bone within the block?....Thanks..Tom Reese ============================================================ In the mid-1960's, Barry Friedman, MD, an orthopedic surgeon with a joint project involving Cleveland Clinic and Case Institute, was thin sectioning bone with diamond knife ultramicrotomy. It was my perception that this was a "first" and had not been done previously. The hydroxy apatite crystals most certainly did do damage to the knife edge, and that was a forgone conclusion, but the important thing was to develop the technique to the point that minimum knife damage would result. At that time, Friedman used the only type diamond knives that were available, and those were what today we would call "life science" diamond knives.
For this kind of work today, since the first "slice" will put striations (from the hydroxy apatite crystals) into a knife's edge, one should consider the use of "materials science" diamond knives.
Disclaimer: SPI Supplies offers materials science diamond knives which can also be used on hard tissue life science samples.
Chuck
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