Rick Felten-at-MACDERMID 12/01/99 08:45 AM I have been measuring the %P in electroless Ni for several years. I have done some recent work where the numbers that I a getting are 20% lower than the accepted wet method. I can't imagine what I am missing. I use standards (Pure Ni and GaP (about 30%P)). I measure the beam current from the SEM. The current drift is typically around 1%. I even normalize my data for current drift. My standards have a %rel std dev of about 1%. I choose several point per sample and standard. Some sample I run un-coated. In such a run the only thing coated is my manufactured GaP standard, that has 200A of carbon. It hasn't been re-polished in 6 years, but that should not give higher P cts in my std. I have tried manually performing my background subtraction subtracting around the P peak, with only a 3% improvement. I get an analytical total typically 95-100% (%P + %Ni). This should be a sanity check for precision and accuracy. I collect data on my standards each time I run a new sample so tilt or detector window condensation should have the same effect. The range for %P recently has been 5-8% which gives a healthy peak. What could I be missing? Any help would be appreciated. Thanks Ric
I don't know much about your sample or standard, but I would wonder if there is any heterogeneity in the distribution of P in either your sample or standard? I would hope the GaP is quite stable, but is there any chance of formation of a P-rich layer at or near the surface? You aren't probing very deep. Also, how does the P occur in the nickel. Is it present in sold solution only or in inclusions of some kind?
You say your results are 20% low for P. Is that relatively speaking? If so, then it may be that you having standard problems. The 2% or so that you are missing would help your analytical totals if you can find it.
Some quick thoughts. Warren S.
At 08:45 AM 12/1/1999 -0500, you wrote: } Rick Felten-at-MACDERMID } 12/01/99 08:45 AM } I have been measuring the %P in electroless Ni for several years. I have } done some recent work where the numbers that I a getting are 20% lower than } the accepted wet method. I can't imagine what I am missing. I use } standards (Pure Ni and GaP (about 30%P)). I measure the beam current from } the SEM. The current drift is typically around 1%. I even normalize my } data for current drift. My standards have a %rel std dev of about 1%. I } choose several point per sample and standard. Some sample I run un-coated. } In such a run the only thing coated is my manufactured GaP standard, that } has 200A of carbon. It hasn't been re-polished in 6 years, but that should } not give higher P cts in my std. I have tried manually performing my } background subtraction subtracting around the P peak, with only a 3% } improvement. I get an analytical total typically 95-100% (%P + %Ni). This } should be a sanity check for precision and accuracy. I collect data on my } standards each time I run a new sample so tilt or detector window } condensation should have the same effect. The range for %P recently has } been 5-8% which gives a healthy peak. What could I be missing? } Any help would be appreciated. } Thanks } Ric
Again some items for free, that is, if you can arrange pick up.
I am disposing of a complete ETEC Autospec WD X-ray Spectrometer. The spectrometer with crystals and separate electronic console containing: Detector Control Crystal Select (LiF, PET, LOD, RAP), Spec.Pos.Control, Scaler Timer, Amplifier -PHA' Linear Ratemeter. This WDS was originally attached to our ETEC Autoscan SEM, which (just a point of general interest) is still working satisfactory after 26 years of usage.
Cheers
Hans Brinkies
Hans G Brinkies Senior Lecturer Swinburne, University of Technology School of Engineering and Science Electron Microscopy Hawthorn, 3122, Melbourne - Australia
I have a glass (leaded glass) tube, about 0.5 cm in diameter. The inside wall of the tube has been leached leaving behind a porous silica network with pores about 500 nm in size. The thickness of the leached layer is estimated to be about 400 nm. We would like to prepare cross sections to look at the glass/leached layer interface and to be able to actually measure the thickness of the leached layer. I'm wondering if anyone has suggestions or experience on preparing samples such as these for TEM ?. Tripod polishing is a possibility , but the leached layer might not survive the polishing. Has anyone tried microtomy with this type samples?.
Can anyone suggest a good text book on light microscopy, principles, techniques etc ? It will be great if the particular book includes chapters on fluorescence microscopy. Otherwise you can also give me the title for a separate book on fluorescence microscopy.
Thanks in advance.
Soumitra
Soumitra Ghoshroy Ph.D. Electron Microscopy Lab Box 3EML New Mexico State University Las Cruces, NM 88003-8001 Tel: 505-646-1531/3600 Fax: 505-646-5665 e-mail: ghoshroy-at-nmsu.edu
We have a Reichert Ultracut E with an FC4D cryo unit. Lately I have been under the impression that the reservoir refilling cycle is taking much longer than it used to.
I was wondering if others with the same unit could give me their impression as to whether or not the behavior I describe bellow seems normal.
When I first start the unit with a filled LN dewar, the first two reservoir refilling cycles(by this I mean the time it takes to go from only one indicating green light on to all green lights on) take only a few minutes ( maybe five minutes), but after that the time to refill the reservoir decreases considerably. After an hour or so of using the unit, with the temperature (knife and sample) stabilized at about -100C, it takes close to 25 min. to refill the reservoir. I also notice that the temperature of the knife and sample changes from a low of -100C (with the reservoir full) to -94 with the reservoir empty (i.e. when the refill just starts). If someone would let me know if this seems normal I would appreciate it.
I am looking for an epifluorescence equipment to fit our OM Zeiss Universal. Would anyone care to trade or sell those parts I need? - the 50 W lamp illuminator equipment (or 100 W) - the epifluorescence' nosepiece (whitout the Neofluar objectives). - not indispensable: exciter and barrier filters (BG12 & 53/44; BG3 & 50/44)
We would appreciate any info on the track record of these OM products. My request to the Carl Zeiss web site was processed, but without result after tree weeks. Thanks in advance
JOSE ULLAN E-mail: jullan-at-mixcoac.upmx.mx ----------------------------------- Dept. ANATOMIA E. de MEDICINA Univ. Panamericana http://www.mixcoac.upmx.mx
If I recall the legends correctly it was some sort of 'growth' Ed Sharpe
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } I am wondering if this is the infamous 'CD Rot' condition that was } prophesied at the dawn of the CD era. } } Ed Sharpe archivist for SMECC }
Ed,
OK I'll bite. Tell us more about this 'CD Rot'. I think not leaving them lying around exposed to direct sunlight or even strong daylight for years on end would be a reasonable precaution to take.
(Does anybody know of a suitable fixitive ;)
Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457 Ansto Materials Division Fax: 61-2-9543-7179 PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au Australia www: http://www.ansto.gov.au/
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} From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk} To: "Marti, Jordi" {Jordi.Marti-at-alliedsignal.com}
I have some experience with the original FC4. How about checking the state of the seal between the pump and the dewar? Ours cracked and was replaced with an O-ring - not ideal but it works - maybe you are losing filling pressure? The electronics should either work or fail - that is just my guess.
Also, maybe check the hose pipe connections for wear or damage - again with pressure retention in mind.
Keith Ryan _________
_______________________________ Dr. Keith Ryan Marine Biological Association of the UK The Laboratory Citadel Hill Plymouth Devon PL1 2PB England
Tel. ++44 (0)1752 633249 Tel. ++44 (0)1752 633279 The 279 number has an answering machine
Marti, Jordi wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hello: } } I have a glass (leaded glass) tube, about 0.5 cm in diameter. The inside } wall of the tube has been leached leaving behind a porous silica network } with pores about 500 nm in size. The thickness of the leached layer is } estimated to be about 400 nm. We would like to prepare cross sections to } look at the glass/leached layer interface and to be able to actually measure } the thickness of the leached layer. I'm wondering if anyone has suggestions } or experience on preparing samples such as these for TEM ?. Tripod } polishing is a possibility , but the leached layer might not survive the } polishing. Has anyone tried microtomy with this type samples?. } } Suggestions are welcomed. } } Thanks } } Jordi Marti
Jordi, With a pore size of 500nm, have you considered looking at a fracture mounted such that the original tube would be vertical and using an SEM? I would try coating with carbon, followed by gold (or Au/Pd), and use as low a kV as possible.
Ken Converse Quality Images third party SEM service Delta, PA
Hans Brinkies wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Again some items for free, that is, if you can arrange pick up. } } I am disposing of a complete ETEC Autospec WD X-ray Spectrometer, ie. } the spectrometer with crystals and separate electronic } operational console containing; Detector Control } Crystal Select (LiF, PET, LOD, RAP) } Spec.Pos.Control } Scaler Timer } Amplifier -PHA } Linear Ratemeter. } This WDS was originally attached to our ETEC Autoscan SEM, which } (just a point of general interest) is still working satisfactory } after 26 years of usage. } } Cheers } } Hans Brinkies } } Hans G Brinkies } Senior Lecturer } Swinburne, University of Technology } School of Engineering and Science } Electron Microscopy } Hawthorn, 3122, Melbourne - Australia
Hans, I'm not surprised at all that your Autoscan is still running fine. I'm still servicing #17 (vintage '71 or early '72) that has been abused by hundreds of students. And you can't beat the camera system unless you go to a 16mB file size. I have a number of Autoscans on their second or third owner. Most of the original operators only got rid of them because their management or bean-counters told them they had to. The "new" ones are only about 17 years old.
Keep a good system running!
Ken Converse Quality Images third party SEM service Delta, PA
Hello, Does anyone have experience with immuno em of ribosomal associated proteins, fixation to embedding, pre or post embed label. The If works but we are not sure that our standard IEM protocol (post embed label/ LR White) will work. We would appreciate advise before we dive in . Thanks
First, thanks to all who replied to my inquiry about "spotty CD-Rs." One respondent described a similar problem with CD-Rs (CDQ-74BN) from the same manufacturer. My disks are all CDQ-74CN.
Since posting the inquiry I've inspected CD-Rs written in 9/1999, and one of these showed crystalline spots - which developed since the disk was written and kept in dark storage at moderate T and RH.
This morning I sampled and analyzed the crystalline material using FTIR microscopy. The crystalline sample spectrum compares with reference spectra for bisphenyl-A. The CD-R surface spectrum compares with reference spectra for polycarbonate, bisphenyl-A. I am not knowledgable about the manufacture and composition of CD-Rs, but it seems that bisphenyl-A, or a similar compound, is recrystallizing on the CD-R surface.
Can anyone provide more enlightened interpretation of this data?
i've microtomed some glass/multilayer coating surfaces. this tends to make a mess of the knife (try a used diamond). you need to pick up the "pieces" on a filmed grid and hunt for a good area. the pieces are really fractured instead of cut so there is a lot of variation in them. its really not too difficult, but it may end up being costly.
good luck! b- ************************************************************ } } } Hello: } } I have a glass (leaded glass) tube, about 0.5 cm in diameter. The inside } wall of the tube has been leached leaving behind a porous silica network } with pores about 500 nm in size. The thickness of the leached layer is } estimated to be about 400 nm. We would like to prepare cross sections to } look at the glass/leached layer interface and to be able to actually measure } the thickness of the leached layer. I'm wondering if anyone has suggestions } or experience on preparing samples such as these for TEM ?. Tripod } polishing is a possibility , but the leached layer might not survive the } polishing. Has anyone tried microtomy with this type samples?. } } Suggestions are welcomed. } } Thanks } } Jordi Marti
Pardon me if someone has already addressed this inquiry, but what did the manufacturer say about the "spotty CD-Rs"??? They should at least have a theory about what you are seeing, and might save us some time and speculation, here. I would recommend you speak to them before running any major investigations of your own.
Gregg Sobocinski Parke-Davis Pharmaceuticals Ann Arbor, Michigan USA Gregg.Sobocinski-at-wl.com
-----Original Message----- } From: James Martin [mailto:James.S.Martin-at-williams.edu] Sent: Friday, December 03, 1999 9:45 AM To: MSA listserver
First, thanks to all who replied to my inquiry about "spotty CD-Rs." One respondent described a similar problem with CD-Rs (CDQ-74BN) from the same manufacturer. My disks are all CDQ-74CN.
Since posting the inquiry I've inspected CD-Rs written in 9/1999, and one of these showed crystalline spots - which developed since the disk was written and kept in dark storage at moderate T and RH.
This morning I sampled and analyzed the crystalline material using FTIR microscopy. The crystalline sample spectrum compares with reference spectra for bisphenyl-A. The CD-R surface spectrum compares with reference spectra for polycarbonate, bisphenyl-A. I am not knowledgable about the manufacture and composition of CD-Rs, but it seems that bisphenyl-A, or a similar compound, is recrystallizing on the CD-R surface.
Can anyone provide more enlightened interpretation of this data?
} Pardon me if someone has already addressed this inquiry, but what did } the manufacturer say about the "spotty CD-Rs"??? They should at least } have a theory about what you are seeing, and might save us some time and } speculation, here. I would recommend you speak to them before running } any major investigations of your own.
Repeated calls to the manufacturer ended in a voice mail tree. I have reported the problem, but have not received a reply.
What has been an inconvenience for me, may be a more serious problem for other list members who use CD-R but are unaware of this potential deterioration. Empirical observations showed that crystals can form after CD-Rs are written and stored, rendering data inaccessible. My series of analyses took little time - the beauty of FTIR microscopy - but suggested an experimental composition and source of the crystals.
So ... just be aware.
Jamie
On Fri, 3 Dec 1999, Sobocinski, Gregg wrote:
} Pardon me if someone has already addressed this inquiry, but what did the } manufacturer say about the "spotty CD-Rs"??? They should at least have a } theory about what you are seeing, and might save us some time and } speculation, here. I would recommend you speak to them before running any } major investigations of your own. } } Gregg Sobocinski } Parke-Davis Pharmaceuticals } Ann Arbor, Michigan } USA } Gregg.Sobocinski-at-wl.com } } } -----Original Message----- } From: James Martin [mailto:James.S.Martin-at-williams.edu] } Sent: Friday, December 03, 1999 9:45 AM } To: MSA listserver } Subject: additional info about spotty CD-Rs } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } First, thanks to all who replied to my inquiry about "spotty CD-Rs." } One respondent described a similar problem with CD-Rs (CDQ-74BN) from the } same manufacturer. My disks are all CDQ-74CN. } } Since posting the inquiry I've inspected CD-Rs written in 9/1999, and one } of these showed crystalline spots - which developed since the disk was } written and kept in dark storage at moderate T and RH. } } This morning I sampled and analyzed the crystalline material using FTIR } microscopy. The crystalline sample spectrum compares with reference } spectra for bisphenyl-A. The CD-R surface spectrum compares with } reference spectra for polycarbonate, bisphenyl-A. I am not knowledgable } about the manufacture and composition of CD-Rs, but it seems that } bisphenyl-A, or a similar compound, is recrystallizing on the CD-R } surface. } } Can anyone provide more enlightened interpretation of this data? } } Jamie } } ------------------------------------------------------------------------- } } James Martin } Director of Analytical Services & Research } Williamstown Art Conservation Center } james_martin.tripod.com/dasr.htm } } Research Scientist in Chemistry } Williams College } james_martin.tripod.com/williams.htm } } *** Please don't send e-mail attachments. Cut-and-paste text into the } body of an e-mail message. *** } }
I am working on the PET/PEN blends. Now I try to get the microsturcture images of this Polymer blends by TEM. However, From the frist experiment, I did not get very good images. Please suggest about "the sample Preparation".
Again I am an unexperienced and very new in this area.
I've used matrigel as well as collagen to coat culture dish and coverslip surfaces so my tissue culture would grow and proceeded to have no problems fixing them for EM. It would help to have more information about the problems you are having. Tina
"Optimizing Light Microscopy for Biological and Clinical Laboratories" was written just for this purpose. Jim Pawley has been using it at both U. WI and UBC; Dave Knecht uses it at UConn. Has learning objectives at the beginning of each chapter as well as short quizzes at the end. Covers both basic principles and has a detailed section on fluorescence... even a bit on confocal.
Classroom discounts are also available. Please see MME-Microscopy.com/education for further details.
CAVEAT: MME does have a commercial interest in this book.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ******************************************************
At 11:40 AM 12/2/99 -0700, Soumitra Ghoshroy wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Could you please let us know the manufacturer? We've got 3 or 4 different brands over the few years we've been burning CDs. How old were these disks?
Regards, Glen
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
You're showing the life sciences general view of sectioning such material, Chris. In what is loosely termed 'materials science' microtomy, this would not be that great a challenge if all that is desired is the thickness. I don't want to steal Phil Swab's thunder, but in case he doesn't see this, the trick would be to break the tube and embed (with good infiltration re the porosity!) a pointed shard with sharp end towards the block face. (Treating the glass with a silane coupling agent - Dow Corning Z-6040 to enhance adhesion would help). Then section away. Phil has used this method for all manner of glasses, plus thin film coatings such as - hold your breath - boron nitride on diamond on silicon substrate (see Microscopy Research and Technique, vol. 31, p. 308, 1995).
I agree with your FIB comments whole-heartedly, but would also not be surprised if tripod polishing worked. Again, good infiltration would be the key, I suspect, though I am no expert on tripoding.
Tom Malis
---------- From: Chris Jeffree [SMTP:cjeffree-at-srv0.bio.ed.ac.uk] Sent: December 03, 1999 4:43 AM To: microscopy-at-Sparc5.Microscopy.Com Subject: Re: Glass Interface
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} From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk} To: "Marti, Jordi" {Jordi.Marti-at-alliedsignal.com} Subject: Re: Glass Interface Copies to: microsopy-at-sparc5.microscopy.com Send reply to: c.jeffree-at-ed.ac.uk Date sent: Fri, 3 Dec 1999 09:37:59 +0000
Dear Jordi Conventional microtomy won't work on this kind of hard brittle friable material. The most effective method to section hard, brittle material such as glass, silica, ceramics, for TEM is to use a FIB. FIB (focussed ion beam) sections are made routinely by the microelectronics industry to examine the structure of their silicon devices and the interfaces between the fabricated layers. I don't know who offers a service of this kind in the US but I could put you in touch with a colleague in UK with whom we collaborate in providing a service for sectioning of devices. Alternatively, I could arrange the whole job
for you, if that would be useful, and I would be willing to quote you for the work.
An alternative to TEM would be to fracture the tube, or cut and polish it to produce a specimen suitable for SEM. Field emission SEM at low kV is especially good for this kind of porous dielectric material, which will be difficult to coat with a continuous conductive layer. Yours sincerely Chris Jeffree } } Hello: } } I have a glass (leaded glass) tube, about 0.5 cm in diameter. The inside } wall of the tube has been leached leaving behind a porous silica network } with pores about 500 nm in size. The thickness of the leached layer is } estimated to be about 400 nm. We would like to prepare cross sections to } look at the glass/leached layer interface and to be able to actually measure } the thickness of the leached layer. I'm wondering if anyone has suggestions } or experience on preparing samples such as these for TEM ?. Tripod } polishing is a possibility , but the leached layer might not survive the } polishing. Has anyone tried microtomy with this type samples?. } } Suggestions are welcomed. } } Thanks } } Jordi Marti
===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk
I've spoken with one or two persons about this but have not collected specifics. Can someone out there recommend (or discourage me from attempting to buy) an automated TEM negative processor? We'd like to be able to somehow take a stack of exposed 3.25 x 4-in. negatives (Kodak SO-163), pop them into a machine, and have them come out developed, dry, and 'archival.'
Please let me know what's out there.
Thank you
Scott Robinson (sjrobin-at-uiuc.edu) Imaging Technology Group Beckman Institute University of Illinois at Urbana-Champaign
On Fri, 3 Dec 1999, tschwach wrote:} } } I've used matrigel as well as collagen to coat culture dish and coverslip } surfaces so my tissue culture would grow and proceeded to have no problems } fixing them for EM. It would help to have more information about the } problems you are having. } Tina } } Tina, I spoke with Kathy about the matrigel, so I think I can relate the concerns. I have had samples where the cells were seeded in a layer of matrigel. The thickness of the layer was somewhere between 3-5mm, and it was in a plastic multi-well plate. Between the thickness of the matrigel, and the fact that plastic friendly dehydrating solvents had to be used, infiltration was less than ideal. Removing the matrigel from the plate was not an option. Now, after the clarification, has anyone had good success getting such a sample embeded? Randy
Randy Nessler rnessler-at-emiris.iaf.uiowa.edu Views expressed are my own.
Dear Listers, Does anyone have a protocol to remove methacrylate plastic from tissue sections using 1-acetoxy-2-methoxyethane? Rosemary
Rosemary Walsh, Manager The Electron Microscope Facility for the Life Sciences, A Shared Technology Facility, The Life Sciences Consortium 1 South FrearLab Penn State University University Park, PA 16802 (814) 865-0212 rw9-at-psu.edu http://www.lsc.psu.edu/stf/em/home.html
I am pleased to announce that the Live-cell Course continues to grow and prosper. We have worked with over 100 students from 22 contries over the past 4 years and the need seems to keep on growing.
3D Microscopy of Living Cells is IN!
We hope that those with a strong interest in this topic will be able to join us in UBC next summer.
Basic info is below but you can get the entire brochure at
=46ifth Annual INTERNATIONAL 10-Day Short Course on
3D Microscopy of Living Cells June 19 - 29, 2000
=46ourth, Post-course Workshop on
3D Image Processing, July 1 - 3, 2000
Organized by Prof. James Pawley, (University of Wisconsin-Madison) (SEE SABBATICAL ADDRESS AT END OF MESSAGE)
in association with Dr. Elaine Humphrey UBC BioSciences Microscopy Facility: University of British Columbia Vancouver, BC, Canada
DATES
Applications must be received by March 1, 2000 Deposit due April 15, 2000 Registration 5:00 - 7:00 PM Sunday, June 18, 2000 =46irst Lecture 7:30 PM Sunday, June 18, 2000 Live-cell Course ends, noon Thursday, June 29, 2000
APPLICATIONS DUE BY MARCH 1, 2000
More information at : http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
or
REGARDING COURSE ORGANIZATION:
Prof. James B. Pawley, (on Sabbatical) Room LG 10, Madsen Building, F-09, University of Sydney, NSW, 2006 Australia
Dr. Elaine Humphrey, Biosciences EM Facility Biosciences Building Univ. of British Columbia 6270 University Blvd. Vancouver, BC, V6T-1Z4
THE PURPOSE OF THE COURSE
Modern methods of 3D light microscopy promise a revolutionary improvement in our ability to view living cells. To help convert this promise to reality for a wider selection of biological scientists, an intensive eleven-day residential course concentrating on all aspects of 3D Microscopy of Living Cells will be held at the University of British Columbia, in June of 2000. The course includes 4 days on 2D techniques, 6 days of 3D techniques and a summary presentation. It covers basic microscopy to the highest level confocal microscopy. (A half-day Pre-course is offered for any who may need to brush up on basic optics!).
Topics include: o Quantitative 2D light microscopy o How to keep your cells alive o 3D imaging in confocal microscopy o Widefield/deconvolution techniques o Two-photon excitation microscopy o Fluorescent and backscattered light signals o Dye design, characteristics and use o Pixelation: The Nyquist Criterion o Lasers and laser tweezers o Objectives and aberrations o Scanning-systems: AODs and mirrors o Optimal pinhole size/photon efficiency o Detectors: operation and performance o Video-rate confocal imaging o Measuring ion concentrations o Display and measurement of 3D data
Morning lecture/demonstrations lead to hands-on laboratory exercises each afternoon that will utilize most of the commercial instruments currently available for 3D microscopic imaging. Students will work in groups of 3 or 4 throughout the discussion and laboratory sessions, and may complete a live-cell 3D study on their own specimens. In the first four years, over 100 students from 22 countries have attended. Last year, 11 separate, 3D microscopical workstations were available for student use under the supervision of a 17-member international faculty. We expect to have even more workstations in 2000. Including manufacturers representatives, the teacher/ student ratio will be almost 2:1.
INTERNATIONAL FACULTY
o Stephen Adams University of California-SD o Ping Chin Cheng SUNY, Buffalo o Rachel Errington University of Oxford o Stephan Hell Max Planck Institute, Goettingen o Ted Inou=E9 Universal Imaging, PA o Iain Johnson Molecular Probes, OR o Larry Keenan Cell Robotics, NM o Ernst Keller Carl Zeiss, Thornwood, NY o Felix Margadant University of Sydney o Tim Murphy University of British Columbia o Sigrid Myrdal Multidimensional Imaging, WA o Jim Pawley University of Wisconsin-Madison o Steve Potter California Institute of Technology o Jason Swedlow University of Aberdeen o Michael Weis Agriculture Canada o Nick White Oxford University
TUITION
Course tuition is $2,150 US and includes lunches. On receipt of 50% deposit, students will receive preliminary group assignments and the textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The tuition fee includes the textbook, a binder of handouts, and tickets for the Opening Reception, the Manufacturer's Reception and the Beach Party. Accommodations and meals other than lunch are not included in the tuition fee. The Pre-course is $100 US.
APPLICATIONS
Applicants will complete a questionnaire to assess knowledge level and field of interest. Enrollment is limited to about 24 participants. Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with basic texts on request to read before the course begins and are encouraged to take the Pre-course on the afternoon of June 18.
Application forms, and other course information from this and past years, can be downloaded from the WWW site at:
Dr. Elaine Humphrey, Biosciences EM Facility Biosciences Building Univ. of British Columbia 6270 University Blvd. Vancouver, BC, V6T-1Z4 Phone: 1-604.822-3354 FAX: 1-604.265-5315 Email: ech-at-unixg.ubc.ca.
Application deadlines:
Application forms are due by March 1, 2000! Deadlines are early to facilitate setting up groups. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2000 to reserve your position. In general, deposit refunds are only be possible if your position can be filled from the waiting list. The remainder of the fees is due at registration.
DATES
Applications must be received by March 1, 2000 Deposit due April 15 2000 Registration 5:00 - 7:00 PM Sunday, June 18, 2000 =46irst Lecture 7:30 PM Sunday, June 18, 2000 Live-cell Course ends, noon Thursday, June 29, 2000
TEACHING PHILOSOPHY
As befits teaching in an area at the boundary of "what is now known," lecturers have been chosen based on their expertise as scientists working in the field rather than because they all agree. They are encouraged more to be provocative than to be prosaic. Students should expect discussion in areas where differences of opinion exist.
Prior to the course, students will be organized into groups and encouraged to communicate by email/phone, about the "Living-cell" group projects that they will pursue during the course and that will be presented to the class on the last day of the course. It has been found that group interactions make best use of students' prior experience and can be very effective in teaching the practical skills covered in a hands-on course of this type.
ARRANGEMENTS FOR LIVE SAMPLES
Students must contact Dr. Elaine Humphrey to make necessary arrangements for the transport and maintenance of cell lines etc. needed for their projects. Organisms linked in any way with human disease are not permitted because of safety considerations. Transport and customs arrangements for living specimens are entirely the responsibility of the student. Students also attending the 3D Image Processing Course, may be able to analyze, process and display some of the 3D data collected from their specimens
The workshop will cover 3D image processing for measurement and display. Enrollment is limited to those attending the 3D Microscopy course. Tuition : $850 US (lunches and snacks incl.)
WHO SHOULD ATTEND?
The course is designed for biologists working with multidimensional and possibly multicolor microscopical data sets. Getting the data is only half of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to store let alone analyze or display. This course is to help students understand the hardware and software aspects of this problem and give them the techniques they need to make the best use of their data.
The course is designed for biologists who need to make measurements on 3D microscopical data sets and then display the results in an effective manner. The course will be taught on SGI, Macintosh and IBM-compatible computers. A wide variety of software designed for the 3D microscopy market will be described, demonstrated and available for use.
Workshop Organizers
o Nick White Oxford University, UK o Felix Margadant University of Sydney, Au
=46aculty
o Ping Chin Cheng State U. of New York, Buffalo o Rachel Errington University of Bristol, UK o Dan Brown Bruxton Assoc., Seattle WA o Chris MacLean Vaytek Inc., IA o Sigrid Myrdal Multidimensional Imaging, WA
PLAN OF INSTRUCTION Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations followed immediately by hands-on laboratory sessions using a variety of workstations. Students will "learn-by-doing" with two to a machine. Lab handouts will describe some specific exercises to be performed on "canned" data sets. Facilities and supervision will be available until 11:00 PM, for students to work on their own data. **************************************** Prof. James B. Pawley, (on Sabbatical) Ph. 61-2-9351-7548/2351 Room LG 10, Madsen Building, F-09, FAX 61-2-9351-7682 University of Sydney, NSW, 2006 Australia JBPAWLEY-at-FACSTAFF.WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
Nestor, I could not have the webmaster do another page, so I am sending the message again:
The Microscopy Center at Lehigh University wishes to hire a person who has computing and electronic skills to work with electron microscopes. Training on the microscopes will be given if needed. The formal job description can be found on our website at www.lehigh.edu/~inhro/jobs.html.
We are looking for a person with general computer skills (software and hardware familiarity with Windows and Macs) and preferably with some electronic skills ( general maintenance and repair of power supplies and other circuits).
The person appointed will work with two other staff members to maintain and operate the electron microscopes of the Center; will train students in the use of the microscopes and other equipment; will assist the department of Materials Science and Engineering in its computer needs; will develop remote control systems for the electron microscopes and, in general, will handle a variety or related problems.
Lehigh University offers a generous benefit package and competitive salary.
Send resume and letter of interest along with salary requirements and references to Jennifer Mohney, Human Resources, 428 Brodhead Avenue, Bethlehem, Pa 18015
also look at some of the other invisible sample links in the TEM section of Tips & Tricks
http://www.biotech.ufl.edu/~emcl/tips.html
Basically use eosin B or fast green
At 12:19 PM 11/30/99 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I've spoken with one or two persons about this but have not collected specifics. Can someone out there recommend (or discourage me from attempting to buy) an automated TEM negative processor? We'd like to be able to somehow take a stack of exposed 3.25 x 4-in. negatives (Kodak SO-163), pop them into a machine, and have them come out developed, dry, and 'archival.'
Please let me know what's out there.
Thank you
Scott Robinson (sjrobin-at-uiuc.edu) Imaging Technology Group Beckman Institute University of Illinois at Urbana-Champaign
I've spoken with one or two persons about this but have not collected specifics. Can someone out there recommend (or discourage me from attempting to buy) an automated TEM negative processor? We'd like to be able to somehow take a stack of exposed 3.25 x 4-in. negatives (Kodak SO-163), pop them into a machine, and have them come out developed, dry, and 'archival.'
Please let me know what's out there.
Thank you
Scott Robinson (sjrobin-at-uiuc.edu) Imaging Technology Group Beckman Institute University of Illinois at Urbana-Champaign
For those of you attending Cell Biology (Dec 10-15, Washington DC Convention Center): Dr. Manfred Hubert and Dr. Steven Ross (U. Toronto) will be available to answer questions regarding fluorescence in the EFOS booth, #546. We have found them a valuable resource on safety issues (ex: ozone reduction), spectral characteristics of fluorescence systems, and photobleaching.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education}
Anne Marie Cooper Erik Pauls Geoff Avern C. Michael Stanley
Thanks in advance!
Aloha, Tina
http://www.pbrc.hawaii.edu/bemf/microangela
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
In going through some lights I bought at the university auction I came up with and old Leitz projection microscope. A poor jpg is at http://www.couger.com/images/leitz.jpg
It is a cast iron X frame with loupe like optics of about 6 & 12 power It has a condenser,lamp house and helical focus. It is Marked Ernst Leitz Wetlser Germany. The only other mark I can find it Germany on one lens. The body is enameled brass. From the look I would say it was made very early this century or late last. It stands about 9 inches tall
Does anyone have any information on this? It seems to work great.
Thanks Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger
I asked about a photoquality printer that was networkable and faster than an inkjet. Here is a summary of the replies.
****************
You might consider a Tektronix 840P solid-ink printer. We use this printer for routine printing of reports, spectra, images, and transparencies. The printer is much faster than the Epson 740/750 and prints on plain paper, with surprising image quality. The cost is about $3000. For photo-quality prints we still rely on the Epson printers.
We decided on the Tektronix after evaluating Sony and Kodak full-page dye-sub printers and the Pictography.
*****
..our department has a number of networked Tektronix Phaser printers at various levels of quality. They are generally a good company, although when the ink, etc. components run out they're pretty expensive. I think we have a Phaser 330 and 550 that are decent intermediate printers. Cost probably in range of $1000-$2000 (just guessing because our network administrator handles all this). I also have the Epson Stylus Photo in my lab, and while it is quicker per print than the Phaser 550, it doesn't handle either multiple prints (} 5) or any transparencies very well. The Phaser 550 gives better overall quality prints than the Epson, does multiples well, and does an excellent job with transparencies. You may want to check their web site at: http://www.tektronix.com
*****
Alps makes a dye sub printers which are very good. We have the MD 1300. The photo quality images are excellent, but they require the special paper and ribbons from Alps. These printer are not very expensive ( { $400), but they are relatively slow (5 to 10 minutes /print). The Epson Stylus Photo printers are much faster, but have a dottier image.
*****
We use a dye-sublimation printer for images. The one nice advantage that this type of printer offers is the ability to make publication-ready glossy prints (on glossy paper anyway).
The resolution of the printer is 300 DPI, which is more than enough resolution for most journals; who tend to reduce the prints anyway.
*****
Alps makes a relatively inexpensive (~$700US) dyesub. There are some new inkjet printers which, when used with the proper paper and new archival inks, , make not only beautiful images but also permanent ones. There is a review of the latest inkjets in the Computershopper magazine (some go as high as 2400x1200 dpi) and if you do a web search on"quad-black" you will find references to archival inks and photographic papers.
*****
I am answering your inquiry off list. We market a new product for Epson printers that replaces the three color ink cartridges with three black ink cartridges. For those who need highest quality grayscale printing, this configuration greatly widens the grayscale palette. The ink will be archival when printed on special inkjet photo paper.
The product will only work as a plug-in to PhotoShop and drivers must be developed for each Epson printer individually. If you specify which Epson printer model you would use for your grayscale printing, I'll let you know if that model is supported yet.
*****
If you are not getting any useful responses here, why not try one of the usenet groups that deal with digital photography and/or printers. I've found lots of help there.
I did this and got one useful reply.....
HP, and Tektronics, both make excellent color laser printers. Tektronics is rumored to be selling this part of their business to Xerox however. This is not a bad thing, just thought I'd pass it along. Color lasers produce excellent quality prints, and they are much more durable!
*****************
And what have I done?.....
I sent images to both Tektronix and QMS to print out on their laser printers. QMS Magicolor 2 EX (2400x600dpi, laser, A4) pics were disappointing with horizontal banding on the colour images and unnatural colour (hot pink skin tones), though the EM's looked good. Didn't make a good impression: I'd have expected better when they knew there was a sale imminent. The Tektronix 840P (1200dpi, solid ink) was the most impressive of the Tek range when viewed through a hand lens, but from a distance the prints from the Tek 840DP, 740P (1200dpi, A4, laser) and 780GN (1200dpi, A3, laser) were equally good. Comparing with the same prints on coated paper from the Epson Stylus Photo (720dpi), there was very little difference; the Epson just had the edge. For routine printing of images, the laser printer is quite adequate. For best quality - the Epson. As the 840 prints can't be hot-laminated, I chose the 740. Because the 780, with A3 printing, was tempting but considerably more expensive than the 740, I'm buying an Epson 1200 ink jet as well - don't need A3 often and this way I also get a better photoquality printer than I already have. It's been more than paid for by shopping around for the best price. Because of the cost of the prints on the ink jet, not having it networkable is an advantage in a large group!
Thanks everyone,
Diana
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
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Diana, Thanks a lot for summarizing your responses. However, I am curious as to your original post. Did you specifically set a price limit on the printers? I noticed that you did not have any posts regarding the Codonics=
printers. These are quite expensive ($10-12,000) but give superb publication quality images. The model 1660 is a thermal/dye-sub printer. = You can print thermal images on a special paper at the cost of about $.55 per print. These are very high quality and rival the more expensive dye-sub prints (~$2.00 ea). However, color must be done using the dye sublimation method and can be with or without a protective overcoat (~$1.00 additional cost). The other thing I like about this printer is that it is a stand-alone UNIX printer which permits you to adjust gamma, contrast, and color from the printer end....so you do not have to continually adjust the original file unless you want to go this route. You also can do various printer formating as multiple images to a page from separt files, captions, etc. I=
tend to format pages with different sizes of images in PhotoShop but you can also do this by programming the printer. You can also keep track of each users printing for easy billing. This may be helpful for some networke= d printers. =20 I have no direct interest in this product...just a satisfied customer.
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University =20 1057 Whistler Building West Lafayette, IN 47907-1057
Diana van Driel wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hi all, I just wanted to thank everyone who responded to my post:
"We are cleaning out our shelves and want to try to sell a couple of hundred vacuum tubes of the types used in the power cabinets of the Philips EM-200 transmission EM. Many of the tubes have never been used but we also have some used ones from a number of old power cabinets in storage.
We would like to find out if there is still a market value for the tubes and where we should advertise them so they find a new owner rather than go to the local landfill. We would like to recoup some of the cost of the tubes if possible to help offset installation costs of some new equipment."
I received many responses with some of the best advise to go to the web as there are many vacuum tube venders out there. I did just that and found out that there is indeed a thriving market for vacuum tubes...primarily in the larger power tubes used in the old TEM power cabinets. So don't throw them away!! Do hit the web and you may be surprised at the value of some of these old components.
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
id xma010527; Mon, 6 Dec 99 10:14:47 -0600 Received: by SVARLEXC05 with Internet Mail Service (5.5.2448.0) id {X6ZN316Y} ; Mon, 6 Dec 1999 10:13:48 -0600 Debby Sherman {sherman-at-btny.purdue.edu}
Debby, Have you tried Eosin? Just add enough to color the fixative a nice bright pink, and process as usual. Another way to do it is to add make a 1.5% solution of cobalt chloride in absolute ethanol, and using this as a stock solution, add 10 ml per 100 ml of absolute when processing. this colors the tiny stuff while dehydrating. Both of these methods are common in routine histology labs and do not interfere with immuno. Wanda Shotsberger Harris Methodist Hospital Fort Worth TX ---------- } From: Debby Sherman To: message to: MSA list -----------------------------------------------------------------------.
Hi, I was just given some VERY small roots to be fixed and embedded for ICC. Fixation is not a problem but I am not sure I can keep track of these very tiny root pieces once they are infiltrated and embedded in LR White. I would like to stain them with something that is not soluble in ETOH and will also not interfer with the ICC labeling. Any suggestions? Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
We are tasked with characterizing preparations of dry, powdered, milled bacteria such as spores of Bacillus globigii and B. thuringensis using our JEOL 6300FV. We have been using rather crude methods to transfer some of the powdered preparations to stubs covered with double-sticky tape. These stubs are then sputter-coated by a Denton Desk II. Our primary concern is ensuring that we have transferred a representative powder sample to the stub. That is, are all size ranges of particles represented in the sample. The size range of interest is 1 to 20 microns. Size, shape, and number of particles are the parameters we wish to measure (using, we hope, IP-plus).
Are any of you aware of standard or traditional protocols for handling this kind of material? We are complete IPP novices; any suggestions in properly using this software package to perform our analysis?
/John/
John D. Wright, Ph.D. Microbiologist West Desert Test Center Dugway, UT 84022
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
JoAnn - My two cents .. San Francisco State University (where I am presently writing from, and completing my M.A. thesis) offers a course on Electron Microscopy once a year in the Fall semester (late Aug - December). The course number is Biology 741. Whether they'll take outside students is a topic I cannot give an answer to, but you could investigate this by contacting the person in charge, Gregory Antipa. His phone number is (415) 338-2951. This campus is located in San Francisco, CA near the Stonestown Galleria and near Lake Merced (in the southern part of the city). Nelson Conti P.S. This campus has a world-wide web address: www.sfsu.edu There is considerable information regarding class schedules, etc. on that web page or links within it.
Does anyone have a method for removing epoxy that doesn't involve sodium ethoxide or methoxide? I need to do this so that I can decalcify some blocks that unexpectedly turned out to have more heavily calcified areas than they should have. Thanks.
Does anyone have a preferred method for storage of chromium coated samples? I'd guess that storage under vacuum or in an inert gas is the way to go, but I'm curious as to any "pet" methods folks might have, if any.
Thanks! Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 tindallr-at-missouri.edu http://www.biotech.missouri.edu/emc/
To: microscopy-at-sparc5.microscopy.com } From: ard-at-ansto.gov.au (Arthur Day)
Hello all. We have a LEO 910 TEM of which we want to install a Handy camera system, apart from the available film plate camera. Which one is better? Thanks, KIVAMBE.
Tian Huang wrote: ============================================ Hi, there, Could anybody tell me where I can get cryo-SEM done? Thanks! ============================================ The laboratories of Structure Probe, Inc. have offered, since about 1985, as part of our laboratory analytical services, fast turnaround cryo-SEM services. We use Oxford/Hexland equipment and it is interfaced to a JEOL Model 840 SEM. We would be happy to offer a proposal for any work that you might want to be doing.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
As other EM labs on Oahu have closed down in the past decade, I have willingly scavenged their leftovers. Now I am faced with clearing out my pack-rat space. A question arises about epoxy (mostly) resin components that are from ca. 1992 and have remained unopened. Will any of them still be OK? I have used old stocks of Epon812 that have been fine, but not the anhydrides. I also have an old, unopened GMA kit. Can I still use this stuff, or is it time to have it carted off?
Thanks in advance!
Mahalo, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Clearing out my junk, I find I still have the complete manual for the Philips 75 TEM. Does anyone want it? It has dates on it ranging from 1959 to 1964, and our user book goes through 1973...
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
John D. Wright wrote: ================================================== We are tasked with characterizing preparations of dry, powdered, milled bacteria such as spores of Bacillus globigii and B. thuringensis using our JEOL 6300FV. We have been using rather crude methods to transfer some of the powdered preparations to stubs covered with double-sticky tape. These stubs are then sputter-coated by a Denton Desk II. Our primary concern is ensuring that we have transferred a representative powder sample to the stub . That is, are all size ranges of particles represented in the sample. The size range of interest is 1 to 20 microns. Size, shape, and number of particles are the parameters we wish to measure (using, we hope, IP-plus).
Are any of you aware of standard or traditional protocols for handling this kind of material? We are complete IPP novices; any suggestions in properly using this software package to perform our analysis? ====================================================== It sounds like the SPI Tacky Dot™ Slides would stand a good chance of working in this application. You really should be getting a representative sampling of the particles present. For particles less than about 2-3 um you would probably see some doublets or triplets, based on the use of the 15 um dot size product.
More information about the Tacky Dot Slides can be found on URL http://www.2spi.com/new/tacky.html
Disclaimer: SPI Supplies manufactures under license from DuPont the line of Tacky Dot Slides so we would have a vested interest in seeing high volumes used of these slides.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
Can't offer useful advice but I know the problem. When I started here in 1989 I found that a previous technician must have blown a budget surplus on resin kits. Bad move! In those days a volatile component was supplied in glass vials with plastic pop on lids. Suffice to say they were all empty by the time I started, leaving me with dozens of incomplete resin kits!
Dave
On Mon, 6 Dec 1999 19:04:57 -1000 (HST) Tina Carvalho {tina-at-pbrc.hawaii.edu} wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, all- } } As other EM labs on Oahu have closed down in the past decade, I have } willingly scavenged their leftovers. Now I am faced with clearing out my } pack-rat space. A question arises about epoxy (mostly) resin components } that are from ca. 1992 and have remained unopened. Will any of them still } be OK? I have used old stocks of Epon812 that have been fine, but not the } anhydrides. I also have an old, unopened GMA kit. Can I still use this } stuff, or is it time to have it carted off? } } Thanks in advance! } } Mahalo, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } }
---------------------------------------- Patton, David Email: David.Patton-at-uwe.ac.uk "University of the West of England"
My own experience has shown variable results in using older resins. If the resins (including anhydrides) have not been opened, they may be ok. The main thing to check upon opening is the color--DDSA and NMA discolor quite badly. Another clue is during mixing of the components. Anhydrides that are off will give a distinct orange to red/orange color in the final mixture. However, when in doubt, throw it out (that's my motto).
Roger Moretz Dept of Toxicology Boehringer Ingelheim Pharmaceuticals
} -----Original Message----- } From: Tina Carvalho [SMTP:tina-at-pbrc.hawaii.edu] } Sent: Tuesday, December 07, 1999 12:05 AM } To: Microscopy Listserver } Subject: shelf life of resin components } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, all- } } As other EM labs on Oahu have closed down in the past decade, I have } willingly scavenged their leftovers. Now I am faced with clearing out my } pack-rat space. A question arises about epoxy (mostly) resin components } that are from ca. 1992 and have remained unopened. Will any of them still } be OK? I have used old stocks of Epon812 that have been fine, but not the } anhydrides. I also have an old, unopened GMA kit. Can I still use this } stuff, or is it time to have it carted off? } } Thanks in advance! } } Mahalo, } Tina } } ************************************************************************** } ** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } ************************************************************************** } ** }
There are many advantages to having one general list for all microscopies. However, there are also many disadvantages. My primary interests are in the Physical Sciences, and I am not interested in many of Biological topics that come up. As a consequence the utility of this list for me has dropped a lot as the volume of messages has increased.
I think it is time to split this list into two, one for Biology and one for the Physical Sciences. Of course, emails can still be sent to both lists if the topic is appropriate enough.
Comments?
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
Hello all. We have a LEO 910 TEM of which we want to install a Handy camera system, apart from the available film plate camera. Which one is better? Thanks, KIVAMBE.
At 8:04 AM -0600 12/7/99, L. D. Marks wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Please note new FAX number.
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 (Leaving a phone message at 936-3352 is preferable to 358-7555) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
Unfortunately, when lists split they often fall below critical mass and die. I, too, get more posts that I can't use than I can, but the signal/noise is still much better than on the usenet.
Just my 2 cents.
best regards mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
} Date: Tue, 7 Dec 1999 09:46:39 -0600 (CDT ) } From: "L. D. Marks" {ldm-at-apollo.numis.nwu.edu} } X-Sender: ldm-at-node_4990f } To: Chris Adams {cadams-at-lanl.gov} } Subject: Re: Time for more than one list? } Mime-Version: 1.0 } } Perhaps send this to the mailserver...? } } On Tue, 7 Dec 1999, Chris Adams wrote: } } } That sounds like an excellent idea to me. I for one toss 98% of the } } messages being sent out courtesy of this list because they deal with things } } I care little about including: biological specimen prep., job postings, } } complaints about salaries..... I am also more intested in the physical } } sciences being a Northwestern Materials Science Dept. alum. (MS in } } Materials Science, 1981, Bruce Wessels). } } } } Chris } } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } There are many advantages to having one general list for all } } } microscopies. However, there are also many disadvantages. My } } } primary interests are in the Physical Sciences, and I am not } } } interested in many of Biological topics that come up. As a } } } consequence the utility of this list for me has dropped a lot } } } as the volume of messages has increased. } } } } } } I think it is time to split this list into two, one for Biology } } } and one for the Physical Sciences. Of course, emails can still } } } be sent to both lists if the topic is appropriate enough. } } } } } } Comments? } } } } } } ++++++++++++++++++++++++++++++++++++++++++++++++ } } } Laurence Marks } } } Department of Materials Science and Engineering } } } Northwestern University } } } fax: (847) 491-7820 } } } mailto:l-marks-at-nwu.edu } } } http://www.numis.nwu.edu } } } ++++++++++++++++++++++++++++++++++++++++++++++++ } } } } } } } } } } ++++++++++++++++++++++++++++++++++++++++++++++++ } Laurence Marks } Department of Materials Science and Engineering } Northwestern University } fax: (847) 491-7820 } mailto:l-marks-at-nwu.edu } http://www.numis.nwu.edu } ++++++++++++++++++++++++++++++++++++++++++++++++ }
{fontfamily} {param} Courier {/param} A new EELS and XAS tool to play with:
AT LAST, an EELS and XAS fine structure database exists at=20
http://www.cemes.fr/eelsdb/
The aim is to provide an extensive reference data collection.
This database is a compilation of outer-shell and inner-shell excitation spectra from EELS and X-ray investigations, which will form a catalog of fine structures for materials. It allows anyone to download spectra or to submit data.
The database will be in continual development (data will be updated), and, during the next six months, depending on your remarks, the presentation may be modified. Please feel free to send any question or idea to eelsdb-at-cemes.fr (or to sikora-at-lps.u-psud.fr or to serin-at-cemes.fr).
Data can be submitted automatically via Internet. The parameters of the spectra will be checked before incorporation of the data in the web site. All spectra are welcome, whatever their quality (energy resolution, signal to noise ratio,...). The justification for this is that both high and modest energy resolution information can be useful if these spectra exhibit changes in the fine structure.=20
- Raw data are for the moment preferred (no background subtraction, no plural scattering deconvolution or resolution sharpening. Dark current or gain variation correction is OK). Treated data are accepted when processing permits hidden features to be revealed. Details about the processing are required.
- For outer shells (low-loss domain), reduced thickness: 0.3 { { t/lambda { { 1 in order to offer features with optimised intensity.
- Associated low-loss data, recorded from the same area and in the same conditions (convergence angle, mode, probe size, spectrometer aperture) can also (and should) be submitted to offer users the possibility to correct for thickness effects, when the single scattering profile is needed). You must then submit the low loss data before the core-loss data.
X-Ray:
- 2-column Ascii
Some parameters are required, some others are optional.=20
My early training came from the Royal Microscopical Society in the UK. They routinely meet with joint sessions, bio & mat sci. I have to tell you that each of these groups has a lot to learn from the other and would seriously discourage splitting of this group.
An alternative: put an annotation in the header (ex: Bio: or MatS:.....). That way, those of us who are interested in learning on a broader scale can take advantage of the cross-pollination. Others who would like to stay more focused can just "clump and dump" the "uninteresting" messages.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 08:04 AM 12/7/99 -0600, L. D. Marks wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Since some of us are interested in both biological and physical sciences postings, perhaps a better way to handle this would be to have contributors code the subject with "B" for biological and "P" for physical. We could activate filters to remove any unwanted mailings.
I wouldn't look forward to subscribing to yet another list.
#################################################################### John J. Bozzola, Ph.D., Director Micro-Imaging and Analysis Center 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
I for one toss 98% of the } } messages being sent out courtesy of this list because they deal with= things } } I care little about including: biological specimen prep., job postin= gs, } } complaints about salaries.....
Working in the rubber industry, I discard more then 99% of all the info= rmation listservers send me, including this one. So what! I mean it's not lik= e I have to scrub out each message one character at a time. I read what I find interesting and discard the rest. My time (hope my boss doesn't see th= is) is not so valuable that extra 15 to 30 minutes a day can't be spend on dis= carding unwanted E-mail, and I often find "unrelated" information which is of v= alue to me.
I suspect there is some expense and effort in running and hosting a lis= t server, if you which to start your own, great, but let's not ask other = people to spend their time and effort to save us a few extra key strokes. Let= 's keep this server as it is and if you want, you can start your own list and I= will be happy to subscribe to it.
} L. D. Marks wrote: } } } } } There are many advantages to having one general list for all } } microscopies. However, there are also many disadvantages. My } } primary interests are in the Physical Sciences, and I am not } } interested in many of Biological topics that come up. As a } } consequence the utility of this list for me has dropped a lot } } as the volume of messages has increased. } } } } I think it is time to split this list into two, one for Biology } } and one for the Physical Sciences. Of course, emails can still } } be sent to both lists if the topic is appropriate enough. } } } } Comments? } } } } ++++++++++++++++++++++++++++++++++++++++++++++++ } } Laurence Marks I would vote to keep one general list. You can often pick up useful information from threads you wouldn't ussually read. Plus, I don't think you are going to find another volunteer as good as Nestor to run another list.
Just my opinion, JD Arnott
--
LADD RESEARCH 131 Dorset Lane Williston, VT 05495
TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
I disagree, I find it very helpful to see all of the ideas which people have. If there is something that I am not interested in, the delete = key is only a keystroke away.
William McManus Supervisor Electron Microscopy Facility Department of Biology Utah State University Logan UT 84322-5305
billEMac-at-cc.usu.edu 435-797-1920
-----Original Message----- } From: John F. Mansfield [mailto:jfmjfm-at-engin.umich.edu] Sent: Tuesday, December 07, 1999 7:31 AM To: microscopy-at-Sparc5.Microscopy.Com
I agree, it seems to be time.
At 8:04 AM -0600 12/7/99, L. D. Marks wrote: } -----------------------------------------------------------------------= - } The Microscopy ListServer -- Sponsor: The Microscopy Society of = America
Ann Fook Yang EM Unit, Eastern Cereal and Oilseed Research Centre, Rm 2091, K.W. Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
Phone: 613-759-1638 Fax; 613-759-1701
} } } Barbara Foster {mme-at-map.com} 12/07 12:10 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html=20=
My early training came from the Royal Microscopical Society in the UK. They routinely meet with joint sessions, bio & mat sci. I have to tell = you that each of these groups has a lot to learn from the other and would seriously discourage splitting of this group.
An alternative: put an annotation in the header (ex: Bio: or MatS:.....).=
That way, those of us who are interested in learning on a broader scale can take advantage of the cross-pollination. Others who would like to stay more focused can just "clump and dump" the "uninteresting" messages.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com=20 Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 08:04 AM 12/7/99 -0600, L. D. Marks wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20=
Over the time this list has been in operation, I have often enjoyed reading about things people outside my own field have been doing.
However, the sheer volume of biological postings means that I now delete them all wholesale. By doing this, I am negating any possible advantage of cross-fertilization of ideas by reading the biological postings. It also has the disadvantage that I sometimes miss postings by them being buried in a mass of others (for example, I found Laurie's posting on this topic only after I read John Mansfield's reply).
On the other hand, how many topics are Instrumentation rather than materials or biological? For example, digital imaging issues are cross-discipline. Are all such postings to be sent to both lists? Or are we going to set up three lists? If so, should there be only three? Where will it all end?
I think there has to be an EXTREMELY compelling reason to change what is generally regarded as a most helpful, well-organized and successful aid to our work. I have great sympathy for Laurie's point of view. but I don't think it fits the description of an EXTREMELY compelling reason for change.
Tony G-R
} } } There are many advantages to having one general list for all } microscopies. However, there are also many disadvantages. My } primary interests are in the Physical Sciences, and I am not } interested in many of Biological topics that come up. As a } consequence the utility of this list for me has dropped a lot } as the volume of messages has increased. } } I think it is time to split this list into two, one for Biology } and one for the Physical Sciences. Of course, emails can still } be sent to both lists if the topic is appropriate enough. } } Comments? } } ++++++++++++++++++++++++++++++++++++++++++++++++ } Laurence Marks } Department of Materials Science and Engineering } Northwestern University } fax: (847) 491-7820 } mailto:l-marks-at-nwu.edu } http://www.numis.nwu.edu } ++++++++++++++++++++++++++++++++++++++++++++++++ } } }
One list only, please! For all the reasons given today and in the past.
Aloha, Tina
Surf's up, weather's fine. Now if I could only get out of this EM lab...
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
Greetings, As long as the subject line is used wisely, there is little problem. Most folks use the subject line appropriately and it is no big deal to delete, un-read, messages on "tripod polishing" or "Salary comparisons", or whatever. The thing is that each one of us has a unique cross section of interests and expertise, and many would join both lists, causing the more catholic (small c) among us to get even more email. And those who joined just one list would reach a lesser gathering of wisdom.
My backscattered e's, Tobias Baskin _ ____ ^ __ ____ Tobias I. Baskin / \ / / \ / \ \ University of Missouri / | / / \ \ \ Biological Sciences /___/ /__ /___ \ \ \__ 109 Tucker Hall / / / \ \ \ Columbia, MO 65211-7400 USA / / / \ \ \ voice: 573-882-0173 / /____ / \ \__/ \____ fax: 573-882-0123
I would vote for the single list also. The titles of most topics are clear enough to differentiate Physical Sciences from Materials without any further reading and the delete button makes quick work of sorting the things that I'm not interested in.
Nestor does a great job running this one and there are a lot of things that are discussed that cross the materials/physical sciences boundaries (printers, image software, SEM care and feeding, etc.) that may be lost in a multiple list.
I think it's kind of funny we are voting anyway. This list is Nestor's baby and we're here because he takes the time to provide it for us. This list was not established as a democratic entity. There's nothing in it's "constitution" that gives anyone a "vote". His charter is not to listen to the masses and make changes based on their wants. You abide by the rules and if you don't like it you're free to go off and establish your own list at anytime. I'm sure Nestor wouldn't mind a post advertising these two new lists whenever they're established. As for me, I'll stay with this one.
John Giles Principal Materials Engineer Honeywell, Inc.
I have read the posts on having two lists and I too receive many emails that I am not interested in. However, what about general microscopy questions? There seems to be many of them and they are usually answered by either biologists or material scientists. I would suggest instead setting up some rules for posting in the subject heading. That way emails can easily be filtered through Outlook. For example BIO should begin each biological post and MAT and GEN can begin each materials and general post. This way there is one list and each individual can decide on the content that he wants by filtering out the posts. What does everybody think? Of course this is all subject to Nestors approval. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif ____________________________________________
Dear Listservers: I agree with Mark. Some cross-discipline microscopy never hurt anyone...I don't think. Regards, Mike Coviello
"Dr. Mark W. Lund" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Laurence, } } Unfortunately, when lists split they often fall below } critical mass and die. I, too, get more posts that } I can't use than I can, but the signal/noise is } still much better than on the usenet. } } Just my 2 cents. } } best regards } mark } } Mark W. Lund, PhD } VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { } MOXTEK, Inc. } 452 West 1260 North } Orem UT 84057 801-225-0930 FAX 801-221-1121 } lundm-at-xray.byu.edu } } "This is a YOUNG business...How can I tell you what } YOUR job is when I don't know what MINE is?" --Pogo
John: To count and sort in IPP, you will first need to calibrate your specimen from pixels to microns, then threshold to select just the items you want to sort. After that, measure them, then sort by whatever criteria you wish to use. It is a fairly straightforward process, once you figure out where the buttons are for all these steps. Feel free to contact me if you think I can help Wanda Shotsberger Harris Methodist Hospital Fort Worth TX. ---------- } From: Wright, John D. To: 'Microscopy List Server (E-mail)' -----------------------------------------------------------------------.
Ladies and Gentlemen,
We are tasked with characterizing preparations of dry, powdered, milled bacteria such as spores of Bacillus globigii and B. thuringensis using our JEOL 6300FV. We have been using rather crude methods to transfer some of the powdered preparations to stubs covered with double-sticky tape. These stubs are then sputter-coated by a Denton Desk II. Our primary concern is ensuring that we have transferred a representative powder sample to the stub. That is, are all size ranges of particles represented in the sample. The size range of interest is 1 to 20 microns. Size, shape, and number of particles are the parameters we wish to measure (using, we hope, IP-plus).
Are any of you aware of standard or traditional protocols for handling this kind of material? We are complete IPP novices; any suggestions in properly using this software package to perform our analysis?
/John/
John D. Wright, Ph.D. Microbiologist West Desert Test Center Dugway, UT 84022
I agree that unopened epoxy items should ok. We have used resins that are VERY old. I am currently using donated Spurr (Medium) pre-weighed mixture from June 1991. We were using some old TAAB Embedding Resin from 1983 a few months ago - it worked fine. I found a problem with "bulk" bottles when I uswed to weigh out mixtures from e.g. 250 ml bottles. The anhydrides increased in viscosity as they convert by hydrolysis to the acids. Also, opened bottles of accelerator do go "off" after some years.
-From: L. D. Marks {ldm-at-apollo.numis.nwu.edu} } } There are many advantages to having one general list for all } microscopies. However, there are also many disadvantages. My } primary interests are in the Physical Sciences, and I am not } interested in many of Biological topics that come up. As a } consequence the utility of this list for me has dropped a lot } as the volume of messages has increased. } } I think it is time to split this list into two, one for Biology } and one for the Physical Sciences. Of course, emails can still } be sent to both lists if the topic is appropriate enough. }
My interest are in LM and even less info is on this list about this. But I am very strongly for one list. It is a small list that generates less mail than I get on get rich quick spam every day. You don't have to read them all.
The advantage to one list is the interdisplanary answeres to questions And this is the best list I have found for this.
Please do FIX it is isn't broken.
Gordon Gordon Couger 624 Cheyenne Stillwater, OK 74075 405 624 2855 GMT - 6:00
In a message dated 12/7/99 2:45:44 PM Eastern Standard Time, jegiles-at-space.honeywell.com writes:
{ { Subj: RE: Time for more than one list? Date: 12/7/99 2:45:44 PM Eastern Standard Time From: jegiles-at-space.honeywell.com (Giles, John E Jr (FL51)) To: Microscopy-at-Sparc5.Microscopy.Com (Microscopy List)
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
I would vote for the single list also. The titles of most topics are clear enough to differentiate Physical Sciences from Materials without any further reading and the delete button makes quick work of sorting the things that I'm not interested in.
Nestor does a great job running this one and there are a lot of things that are discussed that cross the materials/physical sciences boundaries (printers, image software, SEM care and feeding, etc.) that may be lost in a multiple list.
I think it's kind of funny we are voting anyway. This list is Nestor's baby and we're here because he takes the time to provide it for us. This list was not established as a democratic entity. There's nothing in it's "constitution" that gives anyone a "vote". His charter is not to listen to the masses and make changes based on their wants. You abide by the rules and if you don't like it you're free to go off and establish your own list at anytime. I'm sure Nestor wouldn't mind a post advertising these two new lists whenever they're established. As for me, I'll stay with this one.
John Giles Principal Materials Engineer Honeywell, Inc.
----------------------- Headers -------------------------------- Return-Path: {Microscopy-request-at-sparc5.microscopy.com} Received: from rly-yd02.mx.aol.com (rly-yd02.mail.aol.com [172.18.150.2]) by air-yd01.mail.aol.com (vx) with ESMTP; Tue, 07 Dec 1999 14:45:44 -0500 Received: from Sparc5.Microscopy.Com ([206.69.208.10]) by rly-yd02.mx.aol.com (v66.4) with ESMTP; Tue, 07 Dec 1999 14:45:31 1900 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id NAA08076 for dist-Microscopy; Tue, 7 Dec 1999 13:18:31 -0600 Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id NAA08065 for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 7 Dec 1999 13:17:59 -0600 Received: from fl51m03.space.honeywell.com (fl51m03.space.honeywell.com [130.181.6.220]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id NAA08057 for {Microscopy-at-MSA.Microscopy.com} ; Tue, 7 Dec 1999 13:17:39 -0600 Received: by fl51m03.space.honeywell.com with Internet Mail Service (5.5.2650.21) id {YG1J3HT4} ; Tue, 7 Dec 1999 14:18:09 -0500 Message-ID: {BA5F9C6AA352D311AD4E009027855BCD01EF7DAC-at-fl51m01.space.honeywell.com} From: "Giles, John E Jr (FL51)" {jegiles-at-space.honeywell.com} To: Microscopy List {Microscopy-at-Sparc5.Microscopy.Com} Subject: RE: Time for more than one list? Date: Tue, 7 Dec 1999 14:16:47 -0500 MIME-Version: 1.0 X-Mailer: Internet Mail Service (5.5.2650.21) Content-Type: text/plain; charset="iso-8859-1" Errors-to: Microscopy-request-at-sparc5.microscopy.com
Amen to the two Johns. I am mostly biological, but get some tips from the physical notes. If I'm not interested in the topic, I just hit the delete key. I'm sure I wouldn't take the time to log on to a different site for the physical comments; thus, I'd miss some useful stuff.
This is a good time to remind users to be concise and clear in the subject line. A "B" or "P" is not a bad idea either.
Sara
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
} --- } } } I agree, it seems to be time. } } At 8:04 AM -0600 12/7/99, L. D. Marks wrote: } } ... } } I think it is time to split this list into two, one for Biology } and one for the Physical Sciences. Of course, emails can still } be sent to both lists if the topic is appropriate enough. } } Comments?
So far today ... these 2 messages amount to half my "microscopy list" e-mail. If it were a busier list, I might agree ... but personally, I don't have a problem with recognizing the posts I'm not interested in and deleting them. I believe there is too much on this list regarding general microscopy, as applied to all sciences, that I'd be afraid to miss if I joined a "materials' list".
Which is not to say ... some of you could do a better job with focussing the subject line ... some mails you have to open to realize what's inside.
cheerios, shAf
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {} Michael Shaffer, R.A. - ICQ 210524 Geological Science's Electron Probe Facility - University of Oregon mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/
I agree with Barbara. Although I just scan many of the messages that request specific information about something I know nothing about, I personally enjoy reading about new stuff and learning from it. I believe shattering this list into 2 or more sublists is counterproductive.
I have set up my email application so that every mail with "www.msa.microscopy.com" in the text gets automatically transferred into a separate folder. When I have a few minutes (not often these days!!), I sift through the messages, delete the ones that deal with subjects that I don't track and move the others into a "keep" folder. It's really not a lot of work and I know most of the time by the subject line what I want to keep or throw out.
Just my humble opinion.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: Barbara Foster[SMTP:MME-at-MAP.COM] } Sent: Tuesday, December 07, 1999 10:10:17 AM } To: L. D. Marks; Microscopy List } Subject: Re: Time for more than one list? } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mark,
My early training came from the Royal Microscopical Society in the UK. They routinely meet with joint sessions, bio & mat sci. I have to tell you that each of these groups has a lot to learn from the other and would seriously discourage splitting of this group.
An alternative: put an annotation in the header (ex: Bio: or MatS:.....). That way, those of us who are interested in learning on a broader scale can take advantage of the cross-pollination. Others who would like to stay more focused can just "clump and dump" the "uninteresting" messages.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 08:04 AM 12/7/99 -0600, L. D. Marks wrote: } ----------------------------------------------------------------------- - } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I wanted a moderately fast networkable printer and specifically mentioned lasers, photoquality and not too expensive. Prices in Aus are higher than in the US, so what is expensive to you is very expensive for us. The laser printers have the advantage of being able to do routine Departmental printing.
Diana
Diana van Driel Department of Clinical Ophthalmology Sydney University GPO Box 4337 Sydney NSW AUSTRALIA 2001
We are having problems with terrible background when immunogold labelling sections on slot grids using either formvar or butvar as support films.. Using the exact same procedure on 300 hexagonal grids with the same support film background is not a problem.
The background in on the resin, all the cells and support film.
Has anyone else experienced this problem using slot grids?
Manuela Palatsides Electron Microscopy Peter MacCallum Cancer Institute Locked Bag#1 A'Beckett Street Melbourne 3000
Hi All, We are in the market for a small film drying cabinet for our EM film. We don't need, or have space for one of thoses locker-sized jobs they sell in photo suplly catalogs. Any ideas on where to look? Or better still, is anyone looking to get rid of one?
Thanks, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Confocal Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
This comment comes up at least once or twice/year generally in private messages to me, but occasionally in the public forum.
Besides wearing out your delete button what are the disadvantages of allowing the interaction of all microscopists, regardless of their background?
The free and mutual exchange of ideas and input ESPECIALLY from those of different disiciplines IMHO far outweighs the minor annoyance of deleting messages. The way our understanding expands is by hearing and listening to what other in different fields say, and from that dialog coming up with new knowledge. The long term benefits of that are potentially too great to ignore. We do not exist in isolation and cannot learn from others if we create isolation. Clearly mixing commentary on a completely unrelated subjects (say automobile repair with microscopy) has very little potential for creating new understanding, but mixing light, electron, probe, and x-ray microscopy/microanalysis between life and physical sciences is a much different issue.
The appropriate use of the SUBJECT field is important in reducing/helping others decide on what to read or not read and I would (as always) encourage better use. But that was discussed when the server started back in 1993 !! Has it really been that long...?? Yep I guess so. In any case it's been alot of Mbytes of texts that's for sure.
So in short, I would disagree with those who think the list should be divided up. I think too much will be lost from cross-fertilization of ideas and concepts. The delete button works very well for me and I probably get alot more messages than most of you on the server.
But, that is my personal opinion. I'm interested in the comments of others on the list and will sit back and listen for a bit to see how the discussion develops within our "little group".
Nestor Your Friendly Neighborhood SysOp.
================================================================== Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." so I bought a G3 Mac
I scan messages by subject, thus reducing to a minimum the number of messages that I start to read. The first few lines of the actual message are another effective filter. This works well for me.
Two lists will not split subjects but rather people (subscribers) into two groups. Since some subjects are of interest to both physical and biological subscribers (ex: instrumentation, printers, scanners, image manipulation, conferences covering both aspects of microscopy, cameras, and some microscopy techniques), such a division may not turn out to be a good thing. Would MSA eventually split into two societies too?
Just writing clear subject lines in every message, and using some discretion in the number of postings as well as in the length of discussions - like this one - will keep the listserver effective for everyone.
True, the list is a very busy place. This can be a bit of a burden, but it is usually easy enough to decide if the topic is of interest to you from the subject, or at least, the title or first line of the text. But I think that this is one of its greatest advantages. We all can share our problems and experience right, here right now. I could see some categorizing of messages. For example, we could require the use of one or more keywords in the subject such as: biological/materials/physical science, or microscope instrumentation/theory. But, if we do separate the list, we will loose a tremendous strength, that is the very depth of the support that is available on this list.
Brad Huggins BPAmoco, Naperville, IL Microscopy & Microanalysis Lab
} ---------- } From: L. D. Marks[SMTP:ldm-at-apollo.numis.nwu.edu] } Sent: Tuesday, December 07, 1999 8:04 AM } To: Microscopy List } Subject: Time for more than one list? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } There are many advantages to having one general list for all } microscopies. However, there are also many disadvantages. My } primary interests are in the Physical Sciences, and I am not } interested in many of Biological topics that come up. As a } consequence the utility of this list for me has dropped a lot } as the volume of messages has increased. } } I think it is time to split this list into two, one for Biology } and one for the Physical Sciences. Of course, emails can still } be sent to both lists if the topic is appropriate enough. } } Comments? } } ++++++++++++++++++++++++++++++++++++++++++++++++ } Laurence Marks } Department of Materials Science and Engineering } Northwestern University } fax: (847) 491-7820 } mailto:l-marks-at-nwu.edu } http://www.numis.nwu.edu } ++++++++++++++++++++++++++++++++++++++++++++++++ } }
Does anyone know of a contract lab in the SF Bay Area that will do TEM of virus particles? I'm not sure if the person I'm asking for would like negative staining or thin sections.
I agree with Barbara Foster's comment. I am working in a branch of biology and though I do not understand the whole of the physical microscopy workings, I enjoy hearing about interesting topics no matter what field. I like the idea to attach a heading to the front of subject lines that would designate messages as Bio, versus Mat. Sci.
Sincerely, Laurie Wallin UCSD Department of Anesthesiology 9500 Gilman Drive, 0629, La Jolla, CA 92093 (858) 822-3271
I have been counting responses either way, some of which have not gone to the listserver. At the moment it is about 3:1 against a split (sigh), with a strong sentiment for better subject lines.
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
I'm a geologist/mineralogist myself, but I find many of the biological postings useful, especially those on sample prep. Certainly experience with printers or archiving is a general topic, as are details on particular microscopes. Me...I'll take the whole enchilada; easy to discard what does not sound useful. Dave Pevear, Houston
"L. D. Marks" wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } There are many advantages to having one general list for all } microscopies. However, there are also many disadvantages. My } primary interests are in the Physical Sciences, and I am not } interested in many of Biological topics that come up. As a } consequence the utility of this list for me has dropped a lot } as the volume of messages has increased. } } I think it is time to split this list into two, one for Biology } and one for the Physical Sciences. Of course, emails can still } be sent to both lists if the topic is appropriate enough. } } Comments? } } ++++++++++++++++++++++++++++++++++++++++++++++++ } Laurence Marks } Department of Materials Science and Engineering } Northwestern University } fax: (847) 491-7820 } mailto:l-marks-at-nwu.edu } http://www.numis.nwu.edu } ++++++++++++++++++++++++++++++++++++++++++++++++
I think some abbreviations to indicate particular microscopy area in the beginning of the "Subject" line will be a plus. It does not hurt the idea of solid ListServer but give us some flexibility to set up our E. mail programs to filter and organize information better. Expanding this idea I would suggest Nestor may establish some "rules" for "Subject" line. For example, abbreviation should have a 4 letters starting with capital and ending with ":". Something like "Bio:"; "Mat:", "Gen:". Capital and ":" or something like that may help to tune up filters more precisely. I think, there are more than 3 categories may be established. If for some reason people do not accept such rules - it did not interrupt ListServer functionality.
_________________________________
Sergey Ryazantsev Ph. D. UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
You can make a cheap one from Plexiglas and a hair dryer. Make it to fit with your film processing rack, with the rack off the bottom (two rods will work or you can make a plenum), put the dryer in the bottom in a hole made to fit with a collar, and you need a top with holes. I was going to have one made here, but the machinist that I put on it made the same thing from stainless steel. More expensive, but it works very well. It has the footprint of about 8"x24". (The hair dryer is along the long axis.)
If you have the money, there is a company called California Stainless that makes some of the ones that are sold in the EM catalogs. You can buy direct from them. Sorry, I don't have the address. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Leona Cohen-Gould [mailto:lcgould-at-mail.med.cornell.edu] } Sent: Tuesday, December 07, 1999 7:00 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: film dryers } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } Hi All, } We are in the market for a small film drying cabinet for our } EM film. We } don't need, or have space for one of thoses locker-sized jobs } they sell in } photo suplly catalogs. Any ideas on where to look? Or } better still, is } anyone looking to get rid of one? } } Thanks, } Lee } } Leona Cohen-Gould, M.S. } Sr. Staff Associate } Director, Electron Microscopy Core Facility } Manager, Confocal Microscopy Core Facility } Joan & Sanford I. Weill Medical College } of Cornell University } voice (212)746-6146 } fax (212)746-8175 } } }
Greetings to everyone with all microscopy interests!
I believe most strongly that there should remain only one list. I am sure that had there been two or more lists I would have missed some important pieces of information. While the amount of traffic on the single list remains manageable (15 - 20 messages a day is easy to handle in a few minutes) lets keep it this way.
Many thanks, Nestor, for your good work.
Regards
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
Leave it the way it is. Better subjects will save us all time. But don't break up the best list for finding a solution to a problem I have found.
HIP Hippo Hip Hooray NESTER
It should be possible to write software to block the mail you don't want sent a the mail server.
It would also be possible to send it to a second mailer and strip out what you don't want or use the filter in your mail client to filter out the unwanted postings.
I might be talked into doing it but I real think that if numbers are a problem go to a digest.
As far as this being a busy list is a joke. While it has the highest signal to noise of any group I have been on it is very low volume list.
I read 50 to 70 messages out ot 250 and answer or originate about 25 a day. 30% of this is joke and other personal stuff. Since I am retired I don't mind a bit.
I agree, one list only please. Cross subject information is a means of broadening our appreciation of the many applications of microscopy. in my lab where we service not only biological materials primarily but also soil and mineral from our geology department the single list is most useful.
Ronald Smith, Plant Sciences Department, University of Western Ontario,
Gordon Couger wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } -From: L. D. Marks {ldm-at-apollo.numis.nwu.edu} } } } } There are many advantages to having one general list for all } } microscopies. However, there are also many disadvantages. My } } primary interests are in the Physical Sciences, and I am not } } interested in many of Biological topics that come up. As a } } consequence the utility of this list for me has dropped a lot } } as the volume of messages has increased. } } } } I think it is time to split this list into two, one for Biology } } and one for the Physical Sciences. Of course, emails can still } } be sent to both lists if the topic is appropriate enough. } } } } My interest are in LM and even less info is on this list about this. } But I am very strongly for one list. It is a small list that generates } less mail than I get on get rich quick spam every day. You don't } have to read them all. } } The advantage to one list is the interdisplanary answeres to questions } And this is the best list I have found for this. } } Please do FIX it is isn't broken. } } Gordon } Gordon Couger } 624 Cheyenne } Stillwater, OK 74075 } 405 624 2855 GMT - 6:00
Larry, I agree, although I'm not the one doing the work maintaining this list. Thanks Nestor! Russ Gillmeister, Xerox
-----Original Message----- } From: L. D. Marks [mailto:ldm-at-apollo.numis.nwu.edu] Sent: Tuesday, December 07, 1999 9:04 AM To: Microscopy List
There are many advantages to having one general list for all microscopies. However, there are also many disadvantages. My primary interests are in the Physical Sciences, and I am not interested in many of Biological topics that come up. As a consequence the utility of this list for me has dropped a lot as the volume of messages has increased.
I think it is time to split this list into two, one for Biology and one for the Physical Sciences. Of course, emails can still be sent to both lists if the topic is appropriate enough.
Comments?
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
Regarding the proposed list split: I myself am involved in both material and biological microscopy. My work = involves identifying sources of animal, vegetable, and mineral "unknowns" = via various light microscopy methods of examination. In my opinion, it = would do well for my purposes to split the list into "TEM" and "Others" = rather than "materials" and "biological". I say this because it seems = that 90% of the emails on this list server are related to TEM applications = which are generally all Greek to me and serve no useful purpose in my = field. Still, if this server remains "as is", my 5 minutes a day deleting = obvious TEM related messages is no big problem. As others have stated, = keeping the Subject narrow and Specific must be the goal if only one site = is to remain available. My 3 cents (inflation adjusted) Mike Bucker Microscopy Principal Consolidated Labs of Virginia =20
} } } "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 12/07 7:09 PM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html=20=
This comment comes up at least once or twice/year generally in private = messages to me, but occasionally in the public forum.
Besides wearing out your delete button what are the disadvantages of allowing the interaction of all microscopists, regardless of their background?
The free and mutual exchange of ideas and input ESPECIALLY from those of different disiciplines IMHO far outweighs the minor annoyance of deleting messages. The way our understanding expands is by hearing and listening to what other in different fields say, and from that dialog coming up = with new knowledge. The long term benefits of that are potentially too great to ignore. We do not exist in isolation and cannot learn from others if we = create isolation. Clearly mixing commentary on a completely unrelated subjects = (say automobile repair with microscopy) has very little potential for creating new understanding, but mixing light, electron, probe, and x-ray microscopy/microanalysis between life and physical sciences is a much different issue.
The appropriate use of the SUBJECT field is important in reducing/helping others decide on what to read or not read and I would (as always) = encourage better use. But that was discussed when the server started back in 1993 !! Has it really been that long...?? Yep I guess so. In any case it's been alot of Mbytes of texts that's for sure.
So in short, I would disagree with those who think the list should be = divided up. I think too much will be lost from cross-fertilization of ideas and concepts. The delete button works very well for me and I probably get alot = more messages than most of you on the server.
But, that is my personal opinion. I'm interested in the comments of others on the list and will sit back and listen for a bit to see how the discussion develops within our "little group".
Nestor Your Friendly Neighborhood SysOp.
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov=20 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D TPMLab: http://tpm.amc.anl.gov=20 MMSite: http://www.amc.anl.gov=20 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
The box said "This program requires Win 95/98/NT or better..." so I = bought a G3 Mac
Perhaps you should read from the list archives the previous discussions about this very same subject.. It appears that this same discussion surfaces every few years, and ends to the same conclusion..
Besides that, the "idea" of LM: EM: etc. prefixes in subject lines is VERY old, and in fact what people SHOULD be doing all the time. RTFFAQ. :) Read the fine FAQ. From FAQ: ".. Also preface your description by the conventional abbreviation of the type of microscopy you are interested such as LM, IRM, XRM, TEM, SEM, AFM, STM, uProbe......"
Just to add something useful to this message, I recently copied all old list archive files into one file (about 50 megabytes), and now if I need to check something, I just do a search to the single file, and find all old discussions.. Very useful. Unix people would use less and / command, PC people list and \ command..
Quick search revealed that this discussion has been on: October 93, May 95, Dec 99..
This thread is a fairly hardy perennial and will be familiar to most members who have been on the list awhile, so just to add my 0.0126 Euro worth.
The concept of specific subjects lines for the list is fine.
Indeed we are asked to do this by Nestor in his original welcome text.
To quote from the saved message (Well, some of us saved it!) :- ====================================================== As a courtesy to the readers of this list please indicate in the subject line of your message a reasonable descriptive title of your comment/inquiry. Also preface your description by the conventional abbreviation of the type of microscopy you are interested such as LM, IRM, XRM, TEM, SEM, AFM, STM, uProbe...... For example, if you are interested in optical microscopy and have a question about staining then a Title/Subject line for your message might be
Thanks to all who responded to the crisis of repairing or replacing our eXL monitor. We were eventually able to locate a local TV repair shop that had it corrected in a couple of days at a relatively meager price of $300.00. It was nice to have some potential options however, should this attempt have failed.
While I'm here. I agree with Nestor's assessment of the listserver objectives. I would much rather filter out the non-relevant information than miss a treasured tip on managing a difficult process or sample prep. The boundaries of science are not that distinct. Remember, everything in life is relative!! My two cents.
============================================ Wayne England Manager, Physical Characterization Bodycote ORTECH Inc. 2395 Speakman Drive, Mississauga, ON, L5K1B3 wengland-at-ortech.on.ca WEB: www.bodycote.com 905-822-4111 Ext.555 FAX:905-823-1446 ============================================
You may not need to remove the expoy to remove the calcium. Depending on how big the block is and how much of it needs to be cut, you may be able to emerse it in EDTA for an extended amount of time to remove enough calcium for sectioning. I have never done this myself, but have seen several people do it over the years. Maybe someone out there with more direct experience will respond.....
Karen
On Mon, 6 Dec 1999, Lesley Weston wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Does anyone have a method for removing epoxy that doesn't involve sodium } ethoxide or methoxide? I need to do this so that I can decalcify some blocks } that unexpectedly turned out to have more heavily calcified areas than they } should have. Thanks. } } Lesley Weston. } } } } } } } }
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Thanks to all who sent suggestions for staining root so it was easier to see when embedded in LR White. Most called for a stain added when tissue was in 100% ETOH. Since I had very little material and did not want= to loose it during dehydration, I decided to try toluidine blue after fixation and prior to ETOH to see if it would tolerate the dehydration, etc= . I was delighted with the end result. A couple of drops of a 1% solution added to the post fix buffer gave sufficient staining after only about 5-10=
minutes to carry all the way through the embedding process. I do not know if there was any effect on immuno-reactivity yet....let's hope not.
In any case, additional suggestions are summarized below. A couple, esp. the method of enrobbing small specimens with formvar (T. Baskin) was especially intriging. I certainly will try that method. This list is an endless stream of good ideas!! Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University =20 1057 Whistler Building West Lafayette, IN 47907-1057
Phluoroglucinol. Excellent stain for lignified structures. Could also use Graff's "C" stain which is a standard for wood and paper fiber structures. Lou Solebello ------------- Re: stain for rootGreetings,=A0=A0=A0=A0=A0=A0=A0 Debby Sherman asked about= handling small bits of root. I make my living handling these snippets so perhaps I can help. =A0=A0=A0=A0=A0=A0=A0 As far as stains go, we have used two. We have made a= saturated solution of Fast Green in ethanol. We make an 8% solution of fast green in 100% ethanol and then add 1-2 =B5l to each half ml of volume with sample. I=
have also used a 0.15 % aqueous solution of basic fuscin. In this case i collect the samples in that prior to dehydration, and they stay nicely purple throughout. =A0=A0=A0=A0=A0=A0=A0 The other thing that I do to greatly simplify my life= is to put each root on a wire loop, encased in Formvar. Here is an excerpt from a lab=
protocol I have: =A0=A0=A0=A0=A0=A0=A0We get best results with a Formvar loop method. In thi= s method, a loop of copper wire (36 gauge) is made and flattened between two flat pieces of steel. Then small rectangles of 0.25% Formvar in ethylene chlorid= e are floated on water and the loop plunged into the middle of the rectangle so that a film of Formvar surrounds the wire loop. A number of loops are made in advance (can be days ahead). A root is then placed on the Formvar surface, the excess cut away with a razor and then this assembly is coated with another Formvar layer, in the same was as above, thus encasing the root between Formvar. This procedure provides better flat embedding than agarose. Up to three loops can be put in a single vial. Note: one does not need super "EM" grade Formvar films, so this is not a hassle at all. =A0=A0=A0=A0=A0=A0=A0When its time to embed, just cut off the stem of the l= oop and embed the loop with the sample on. The loop is heavy enough to sink and kee= p the root nice and flat. The copper is so thin you can cut through it with a=
razor in the block. Or if you like, you can excavate it from the block and pull it out. =A0=A0=A0=A0=A0=A0=A0Alternatively, if you don't want to mess with loops an= d Formvar, you can use agarose. Excise a 3mm tip segment and encase it within a small droplet of 2% low gelling-temperature agarose (Type VII from Sigma). When the agarose sets, the root-containing drop is transferred to 10% ethanol. We get better results with Formvar but the agarose will work. =A0=A0=A0=A0=A0=A0=A0The point of the loops or the agarose is to facilitate= exchange of solutions without loss of samples. It is a snap to suck out the old solution and put in the new with your samples in agarose pellets or held on= to loops. With the agarose, you can keep all of the samples of a single treatment in one vial (this saves time and solution) but with the formvar l= oops, it is hard to use more than three loops per vial. =A0=A0=A0=A0=A0=A0=A0 I hope this helps. Good luck,=A0=A0=A0=A0=A0=A0=A0=A0= =A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0=A0Tobias Baskin p.s. I fix first. I am usually working with arabidopsis roots. We grow the seedlings on agar plates and I pour the fixative right over the seedlings on the plate. This way, they stay submerged and get nicely fixed.= After three rinses, ten min each, I then put the roots on the loops (or encase them in agarose). Because the formvar is so thin, small molecules pass through with no problem at all. We can even get antibodies through although=
these do get held up a bit. ------- You can try eosin. We've used it to impart some color to early chicken embryos that were later labelled by immuno-fluoresent markers. These were embedded im paraffin, and viewed in the LM, but it may cross-over. Leona Cohen-Gould, M.S. ----------- I have tried using fast green to lightly stain Arabidopsis roots (which are quite small) so I could still see them during embedding. I followed the protocol of Baskin et al., Planta 187: 405-413. They used methacrylate for embedding but it works as well in LR white. Just add 1 ul of 8% solution (w/v) fast green dissolved in ethanol when your samples are at the 1st change to 100% ethanol. This should not interfere with immunolabeling. I also used acid fushin but have used this mostly in wax embedded material. Elison Blancaflor --------- Have you tried Eosin? Just add enough to color the fixative a nice bright pink, and process as usual. Another way to do it is to add make a 1.5% solution of cobalt chloride in absolute ethanol, and using this as a stock solution, add 10 ml per 100 ml of absolute when processing. this colors the tiny stuff while dehydrating. Both of these methods are common in routine histology labs and do not interfere with immuno. Wanda Shotsberger -------- We've used both fast green and eosin Y in the 95% ethanol=20 step and been pleased with both. We used fast green to stain isolated protoplasts. Rosemary Walsh
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I think Barbara has a good idea. Some e-mail systems can be set up to automatically dump messages with a certain heading. To me, this would be preferable to a total split.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Mark, } } My early training came from the Royal Microscopical Society in the UK. } They routinely meet with joint sessions, bio & mat sci. I have to tell you } that each of these groups has a lot to learn from the other and would } seriously discourage splitting of this group. } } An alternative: put an annotation in the header (ex: Bio: or MatS:.....). } That way, those of us who are interested in learning on a broader scale } can take advantage of the cross-pollination. Others who would like to stay } more focused can just "clump and dump" the "uninteresting" messages. } } Best regards, } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis. } } At 08:04 AM 12/7/99 -0600, L. D. Marks wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } There are many advantages to having one general list for all } } microscopies. However, there are also many disadvantages. My } } primary interests are in the Physical Sciences, and I am not } } interested in many of Biological topics that come up. As a } } consequence the utility of this list for me has dropped a lot } } as the volume of messages has increased. } } } } I think it is time to split this list into two, one for Biology } } and one for the Physical Sciences. Of course, emails can still } } be sent to both lists if the topic is appropriate enough. } } } } Comments? } } } } ++++++++++++++++++++++++++++++++++++++++++++++++ } } Laurence Marks } } Department of Materials Science and Engineering } } Northwestern University } } fax: (847) 491-7820 } } mailto:l-marks-at-nwu.edu } } http://www.numis.nwu.edu } } ++++++++++++++++++++++++++++++++++++++++++++++++ } } } } } } } } } } }
Are the two types of grids you use made of the same metal? Some metals can cause interferance-probably due to their charge. Are you floating the grids on a drop of solution? If any solution gets to the back side of the grid you could be doubling your background.
I haven't done this stuff for sometime-so my help in this area is limited.
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } We are having problems with terrible background when immunogold labelling } sections on slot grids using either formvar or butvar as support films.. } Using the exact same procedure on 300 hexagonal grids with the same support } film background is not a problem. } } The background in on the resin, all the cells and support film. } } Has anyone else experienced this problem using slot grids? } } Manuela Palatsides } Electron Microscopy } Peter MacCallum Cancer Institute } Locked Bag#1 } A'Beckett Street } Melbourne 3000 } } Telephone: 03 96561244 } Fax: 03 96561411 } Email: m.palatsides-at-pmci.unimelb.edu.au } } } }
You do not mention if your grids are made of the same materials. Copper grids will oxidize during incubation in immunocytochemical reagents, particularly those containing azide (ie, commercial secondary gold conjugates), and also in ammonium bicarbonate, among other buffers. Perhaps there is a difference in the elements which compose your grids?
---------------------- Douglas R. Keene Associate Investigator Shriners Hospital Research Unit 3101 S.W. Sam Jackson Park Road Portland, Oregon 97201 phone: 503-221-3434 FAX: 503-412-6894 (9-5 PST) e-mail: DRK-at-shcc.org
My dear old Kevex 8000 is in need of vintage 1983 dec lsi 1123 circuit boards: cpu board 8186, memory board 8044 and an un-numbered drive interface board with a wide ribbon. Any help would be greatly appreciated!
And: my vote is for one list with subject line indicators. (Great job, Nestor!!!)
Thanks, Dee
Dee Breger Mgr. SEM/EDX Facility Lamont-Doherty Earth Observatory 61 Route 9W Palisades, NY 10964 USA T: 914/365-8640 F: 914/365-8155 E: micro-at-ldeo.columbia.edu
I agree wholeheartedly!!! The answers to many of the questions on this list benefit both the life and material science fields. The questions and answers about printers and cameras, etc. are most beneficial to both fields.
On many mornings I download less than 20 messages, a number easily handled in just a few minutes. I tend to read all of my mail, even though I may not understand it all completely. I feel any new information is helpful, and I enjoy reading and learning about the "materials" side of things as much as the "biological" side. I sincerely hope the listserver stays the same! Jo Dee Fish
Gordon Couger wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } -From: L. D. Marks {ldm-at-apollo.numis.nwu.edu} } } } } There are many advantages to having one general list for all } } microscopies. However, there are also many disadvantages. My } } primary interests are in the Physical Sciences, and I am not } } interested in many of Biological topics that come up. As a } } consequence the utility of this list for me has dropped a lot } } as the volume of messages has increased. } } } } I think it is time to split this list into two, one for Biology } } and one for the Physical Sciences. Of course, emails can still } } be sent to both lists if the topic is appropriate enough. } } } } My interest are in LM and even less info is on this list about this. } But I am very strongly for one list. It is a small list that generates } less mail than I get on get rich quick spam every day. You don't } have to read them all. } } The advantage to one list is the interdisplanary answeres to questions } And this is the best list I have found for this. } } Please do FIX it is isn't broken. } } Gordon } Gordon Couger } 624 Cheyenne } Stillwater, OK 74075 } 405 624 2855 GMT - 6:00
-- Jo Dee Fish Electron Microscopy Assistant The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 858-646-3100 ext.3620
I do echo the sentiment that the subject line should be as=20 descriptive as possible. I think we should all try to follow Joseph=20 Passero's example, he always identifies his subject clearly. Way to=20 go Joe!
At 6:59 PM -0600 12/7/99, L. D. Marks wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Please note new FAX number.
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 (Leaving a phone message at 936-3352 is preferable to 358-7555) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
A colleague wishes to examine pore sizes and distribution in silica-based aerogels by TEM. To thin the sample, we have tried glueing two aerogel samples face to face, then tripod-polishing. We have tried increasing the wedge angle, but still the samples break at the final polishing steps. We have also tried to infiltrate the samples with resin that we cure prior to polishing, to impart some mechanical strength to the pore regions. This too has failed. The aerogels are susceptible to solubilization in acetone and perhaps in polar resins.
We intend to try non-polar resins that are UV-polymerized and continue to try the tripod polishing, and we may dope the resin with osmium (or other mordant) to impart some contrast.
In the meantime, is anyone familiar with prepping this type of sample, or references? Any suggestions?
Thanks Ann Hein Lehman Trinity College EM Facility
We are looking for an independent service company for our Hitachi 7000 TEM. We are considering Materials Analytical Services (Art McKenna), Scientific Instrument Services (Alex Green), Vitaly Feingold Service Company (Atlanta, GA), Pesto Inc., (Gynedd Valley, PA).
If anyone has experience with any of the above, we would so much appreciate hearing from you.
Thank you, Hildegard H. Crowley University of Denver Denver, CO {hcrowley-at-du.edu}
We do much TEM on viruses, both negative staining and thin sectioning. We also get much material FedEx, so that it can be received very quickly. If your customer is interested have him/her email, and I will send details.
Sara Miller (more address info below)
On Tue, 7 Dec 1999, Linda Rangell wrote:
} Date: Tue, 07 Dec 1999 16:25:46 -0800 } From: Linda Rangell {lkc-at-gene.com} } To: MSA {Microscopy-at-sparc5.microscopy.com} } Subject: TEM on viruses } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Does anyone know of a contract lab in the SF Bay Area that will do TEM } of virus particles? I'm not sure if the person I'm asking for would } like negative staining or thin sections. } } Thanks for any information, } Linda Rangell } } }
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
The advantage of tripod polishing is that you get a huge thin area. The disadvantage of tripod polishing is that you get a huge thin area. This translates to the fact that mechanically weak specimens don't have the strength to hold together when thin over large areas.
Inasmuch as these areogel samples have a foam-like structure with brittle cell walls and are more air than specimen, your idea of infiltrating the samples with epoxy is correct. A low viscosity epoxy, like LR White works well (in e.m. supplier catalogs, biological specimens) ((see what you learn reading biological posts as well as phys sci posts!!)). If the epoxy doesn't infiltrate far enough into the sample, then polish the first side the best you can, paint epoxy onto the just-polished face, and make the final first side polish. Soak in LR White to infiltrate into the polished section and the second side polishing should see tha sample hold together. Increasing the wedge angle is also correct--probably a full micrometer turn or more. Try polishing in propylene glycol instead of water. Mount on a small-hole grid.
If you still have problems, leave the specimen about 5 or 10 microns thick and ion mill using a small diameter ion beam. The GATAN PIPS and similar machines now have small beam ion guns that will create an ion milled dimple less than 0.5 mm in size. This way the thicker material surrounding the ion mill dimple provides strength to the thin region. We ***love*** those small ion beams!
Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
Ann wrote:
A colleague wishes to examine pore sizes and distribution in silica-based aerogels by TEM. To thin the sample, we have tried glueing two aerogel samples face to face, then tripod-polishing. We have tried increasing the wedge angle, but still the samples break at the final polishing steps. We have also tried to infiltrate the samples with resin that we cure prior to polishing, to impart some mechanical strength to the pore regions. This too has failed. The aerogels are susceptible to solubilization in acetone and perhaps in polar resins.
We intend to try non-polar resins that are UV-polymerized and continue to try the tripod polishing, and we may dope the resin with osmium (or other mordant) to impart some contrast.
In the meantime, is anyone familiar with prepping this type of sample, or references? Any suggestions?
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I agree with Barbara, one list for all with coding if the consensus goes that way. Wanda ---------- } From: Barbara Foster To: L. D. Marks; Microscopy List -----------------------------------------------------------------------.
Mark,
My early training came from the Royal Microscopical Society in the UK. They routinely meet with joint sessions, bio & mat sci. I have to tell you that each of these groups has a lot to learn from the other and would seriously discourage splitting of this group.
An alternative: put an annotation in the header (ex: Bio: or MatS:.....). That way, those of us who are interested in learning on a broader scale can take advantage of the cross-pollination. Others who would like to stay more focused can just "clump and dump" the "uninteresting" messages.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 08:04 AM 12/7/99 -0600, L. D. Marks wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Thank you all very much for responding to my inquiry about good books on microscopy. Here are the responses I received.
1. Microscopy and Photomicrography - A Working Manual Robert F. Smith, CRC Press, Inc., Boca Raton, Florida, 1990. ISBN - 0-8493-8803-1
2. Light Microscopy in Biology - A Pratical Approach Alan J. Lacey, IRL Press, New York, 1991. ISBN - 0-19-963036-4 (hardbound) ISBN - 0-19-963037-2 (softbound)
The second book contains a special Flourescence Microscopy by P.S. Ploem.
O=B4Brian and McCully (1981): The study of plant structure. Principles and selected methods. Merlbourne Australia. old but good
Light and Electron Microscopy by Elizabeth M. Slayter & Henry S. Slayter. It does mention fluorenscence microscopy but not in great details, but very good of light microscopy in general.
"Video Microscopy" by Sluder & Wolf or "Green Fluorescent Proteins" by Sullivan and Kay. Both available from Academic Press www.apnet.com or "Fluorescence Microscopy" (Microscopy Handbooks, 40); H. J. Tanke, Brian Herman www.amazon.com
"Optimizing Light Microscopy for Biological and Clinical Laboratories" was written just for this purpose. Jim Pawley has been using it at both U. WI and UBC; Dave Knecht uses it at UConn. Has learning objectives at the beginning of each chapter as well as short quizzes at the end. Covers both basic principles and has a detailed section on fluorescence... even a bit on confocal. Classroom discounts are also available. Please see MME-Microscopy.com/education for further details.
Soumitra Ghoshroy Ph.D. Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003 Phone: 505-646-3600/1531 Fax: 505-646-5665 E-mail:ghoshroy-at-nmsu.edu
Project MICRO (Microscopy In Curriculum - Research Outreach) is MSA's middle school outreach program. Many of you have heard of it, but you may not have visited its website (URL below). Now would be a good time, because Nestor, in his abundant spare time, has just posted a major revision. You'll find up-to-date reviews of lots of books, videos & CD-ROMs about microscopy. Most, but not all, are for children; you may get some good holiday gift ideas. There is also a much-expanded website hotlink list. If you are aware of anything that has been missed, please let me know.
I also need potential sources of funding for microscopes for schools. I'm looking for a foundation or program that might be interested in an equipment-only proposal at the $40-50K level. Suggestions?
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
Laurie Wallin says: } I agree with Barbara Foster's comment. I am working in a branch of biology } and though I do not understand the whole of the physical microscopy } workings, I enjoy hearing about interesting topics no matter what field. I } like the idea to attach a heading to the front of subject lines that would } designate messages as Bio, versus Mat. Sci.
Since somebody apparently is counting the opinions (votes?), here's mine.
Splitting the list is a BAD idea, and does not make much sense. Plus, upon what criteria would you have it split? I personally prefer to split by LM vs. EM... See the point...? -- Regards, Uri uri-at-watson.ibm.com -=-=-=-=-=-=- {Disclaimer}
I tend to agree with Barbar and Laurie - and even more so for myself! I am a biological psychologist and the only connection I even have with the group is that I cut mouse brains on an ultramicrotome and then look at them with very simple light microscopy. However, I learn something from some postings, and what I don't want to read I delete - I would be afraid I'd miss something I DO need if you had two lists. Thanks!!
I am only a student member of the MSA, and so I find the discussions on all subjects potentially valuable! So far, my interest is primarily SEM, but I am not sure where my career may take me... just my 1.2 cents worth (students don't have a whole 2 cents to spare) Jenna Brown student, SUNY Potsdam (hi Dr. Rhoads!)
Let me know what you are looking at, and we can discuss your work.
Regards
Pete
Peter Bond Plymouth Electron Microscopy Unit University of Plymouth Drake Circus Plymouth Devon UK PL4 8AA Tel/Fax: 01752 233092 email: pbond-at-plymouth.ac.uk
Is the aerogel closed-cell or open-cell? Closed cell (like styrofoam) is impermeable and won't absorb much resin. Dave Pevear, Houston
"Lehman, Ann" wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } A colleague wishes to examine pore sizes and distribution in silica-based } aerogels by TEM. To thin the sample, we have tried glueing two aerogel } samples face to face, then tripod-polishing. We have tried increasing the } wedge angle, but still the samples break at the final polishing steps. We } have also tried to infiltrate the samples with resin that we cure prior to } polishing, to impart some mechanical strength to the pore regions. This too } has failed. The aerogels are susceptible to solubilization in acetone and } perhaps in polar resins. } } We intend to try non-polar resins that are UV-polymerized and continue to } try the tripod polishing, and we may dope the resin with osmium (or other } mordant) to impart some contrast. } } In the meantime, is anyone familiar with prepping this type of sample, or } references? Any suggestions? } } Thanks } Ann Hein Lehman } Trinity College EM Facility
Perhaps putting a keyword in the subject heading would allow individuals to separate listserv messages into separate files or delete them automatically through functions in e-mail programs. I would like to preserve a commensal relationship. -Karl Garsha ----- Original Message ----- } From: {"r.cross-at-ru.ac.za"-at-sparc5.microscopy.com} To: {microscopy-at-sparc5.microscopy.com} Sent: Tuesday, December 07, 1999 11:27 PM
Surface replicas would be more certain to work; for these you may require a good polish so that the pore distribution is more accurate. A polished (at least a reasonably flat) surface would be easier to produce, because it can be thick and strong. Plastic replicas, because of the solvent requirement would not be possible, but carbon/platinum replicas should work. You probably would dissolve the specimen for cleaning purposes.
If FESEM gives enough resolution (and is available), this could save a heap of trouble.
Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Thursday, December 09, 1999 3:54 AM, Lehman, Ann [SMTP:Ann.Lehman-at-trincoll.edu] wrote: } } } A colleague wishes to examine pore sizes and distribution in silica-based } aerogels by TEM. To thin the sample, we have tried glueing two aerogel } samples face to face, then tripod-polishing. We have tried increasing the } wedge angle, but still the samples break at the final polishing steps. We } have also tried to infiltrate the samples with resin that we cure prior to } polishing, to impart some mechanical strength to the pore regions. This too } has failed. The aerogels are susceptible to solubilization in acetone and } perhaps in polar resins. } } We intend to try non-polar resins that are UV-polymerized and continue to } try the tripod polishing, and we may dope the resin with osmium (or other } mordant) to impart some contrast. } } In the meantime, is anyone familiar with prepping this type of sample, or } references? Any suggestions? } } Thanks } Ann Hein Lehman } Trinity College EM Facility
-----Original Message----- } From: Caroline Schooley {schooley-at-mcn.org} } } } Project MICRO (Microscopy In Curriculum - Research Outreach) is MSA's } middle school outreach program. Many of you have heard of it, but you may } not have visited its website (URL below). Now would be a good time, } because Nestor, in his abundant spare time, has just posted a major } revision. You'll find up-to-date reviews of lots of books, videos & } CD-ROMs about microscopy. Most, but not all, are for children; you may get } some good holiday gift ideas. There is also a much-expanded website } hotlink list. If you are aware of anything that has been missed, please } let me know. } } I also need potential sources of funding for microscopes for schools. I'm } looking for a foundation or program that might be interested in an } equipment-only proposal at the $40-50K level. Suggestions? } Caroline ,
If it is for equipment I would put the arm on the manufactures. They can claim retail as a tax deduction and 30% of retail will cover their cost of production so it is not costing them money. In fact they may make a little. The markup is pretty high in slow turn over items like microscopes.
For those not familiar with the US tax structure the tax rate is from 25 to 34% of profit. So if the tax rate is 30% a 100 dollar tax deduction will save 30 dollars in taxes. If it cost 35 dollars to make an item that retails for 100 and you donate it to a school or other non profit entity you can deduct it from your profit reducing you income tax by $30. The actual cost to the company is 5$
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger
I vote for one list. The reasons have been given and repeated many times already.
This scintillation became a chain-reaction. :-) (the physics part)
Best wishes,
Rado
--------------------------------------------------------------------- Radostin Danev Laboratory of Ultrastructure Research National Institute for Physiological Sciences Myodaiji-cho, Okazaki 444-8585, JAPAN e-mail: rado-at-nips.ac.jp ---------------------------------------------------------------------
INTERNATIONAL CONFERENCE EUTECTICA V 12 - 14 June 2000 , Dniepropetrovsk, Ukraine
ORGANIZING COMMITTEE
National Metallurgical Academy, ave. Gagarin, 4, Dniepropetrovsk, 5, 49600, Ukraine Tel: +380 562 410 602; Tel., Fax :+380 562 676 977; E-mail:mazur-at-dmeti.dp.u EUTECTICA-V International Scientific Conference. 12-14 June 2000
FIRST CIRCULAR
ORGANIZERS ASM International National Metallurgical Academy of Ukraine Division of Materials Science and Metallurgy, Academy of Engineering Sciences of Ukraine
ORGANIZING COMMITTEE Chairman: Prof. Yurii N. Taran, Member, National Academy of Sciences of Ukraine Co-Chairman: Dr. Hans Portisch, President, ASM International Vice-Chairmen: Prof. Vladyslav I. Mazur. Phone: +380 562 41 06 02. Tel./fax: +380 562 67 69 77. Prof. Vladimir I. Shapovalov. Phone: +1 505 275 1625. Secretary: Dr. Svetlana V. Kapustnikova. Phone: +380 562 41 06 02.
ADDRESS National Metallurgical Academy of Ukraine Prospekt Gagarina, 4 Dniepropetrovsk 320635, Ukraine Tel./fax: +380 562 67 69 77. E-mail: mazur-at-dmeti.dp.ua
SCIENTIFIC TOPICS 1. Atomic structure of eutectic melts 2. Mechanism and atomic kinetics of eutectic solidification 3. Influence of cluster structure in melt on kinetics of eutectic solidification 4. Directional solidification of eutectics 5. Three dimensional models of eutectic grains 6. Inoculation and modification of eutectic alloys 7. Thermal stability of eutectic alloys 8. Eutectic alloys properties 9. New eutectic type alloys
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Please use Word for Windows 6.0 or 7.0. For each photo provide a separate file in TIFF or CDR(5.0) and for each simple drawing, a PCX or WMF file. Your floppy disk is returned on request.
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FEE REMITTANCE ADDRESS Mail order: Account ?000071293 LATVIAN TRADE BANK TRIJADIBAS St.,4, RIGA, LV-1048, LATVIA ABN AMRO BANK N.Y., NEW YORK, USA Cor.Acc. ?574072285541, SWIFT: ABNA US 33 Please mark EUTECTICA-V on your order form.
ADDITIONAL INFORMATION 1. The Organizing Committee will appreciate your forwarding this form to your colleagues who might be interested in attending the Conference. 2. Space for sponsors' advertisements will be provided in the Conference Proceedings. The Organizing Committee invites all interested parties to send in their advertising materials.
by ursa.cus.cam.ac.uk with esmtp (Exim 3.12 #1) id 11w0Rg-0000BS-00; Thu, 09 Dec 1999 10:05:09 +0000
The important and interesting advances in science occur at the interfaces of different disciplines. Please keep one list, for as a biologist I am interested to read what non-biologists do in microscopy and analysis. It may mean going through 100 messages to find 1 gem of a new idea, but it's worth it.
Ann Hein Lehman wrote: ============================================================== A colleague wishes to examine pore sizes and distribution in silica-based aerogels by TEM. To thin the sample, we have tried glueing two aerogel samples face to face, then tripod-polishing. We have tried increasing the wedge angle, but still the samples break at the final polishing steps. We have also tried to infiltrate the samples with resin that we cure prior to polishing, to impart some mechanical strength to the pore regions. This too has failed. The aerogels are susceptible to solubilization in acetone and perhaps in polar resins.
We intend to try non-polar resins that are UV-polymerized and continue to try the tripod polishing, and we may dope the resin with osmium (or other mordant) to impart some contrast.
In the meantime, is anyone familiar with prepping this type of sample, or references? Any suggestions? ================================================================ These kinds of samples can be prepared much the same way as a refinery catalyst, or a zeolite system, that is, using
a) standard TEM embedding resins, and b) diamond knife ultramicrotomy
There are several "must do's" in the technique, realizing also that they can be accomplished differently by different users:
• Relatively "hard" block is needed because this is a "hard" sample. We usually would use our own SPI-Pon 812 resin, but some of the other "Epon substitutes" would work, we assume, just as well. I am not aware that we have had such samples dissolving in this resin. There should be enough electron contrast, assuming the sections are thin enough.
• You want to have excellent infiltration, to the degree that (we believe) is generally not possible (at least with certainty) without the use of vacuum impregnation approaches.
• Diamond knife ultramicrotomy, using a "materials science" diamond knife. Naturally we prefer our own SPI diamond knives, but in this case, whatever knife you do use, we believe a 45° angle edge is better than a 55° edge (for this kind of work).
• This has to be done on a good ultramicrotome, and in the hands of an experienced person. One also has to be aware of artifacts, as can occur in any technique, but the principal artifacts here are knife marks and possibly particles pulled out with the knife. But these are easily recognizable as artifacts where as artifacts caused during some of the other potential techniques tend to be more isotropic in nature and therefore more difficult to identify as artifacts.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
I found that there are a lot of usfeful information in all letters and there is not reasonable to split the newsgroup. A lot of informations are interesting to me in the biology despite that I am interested with metals and magnetism, so I would like to remain at only one group. Cheers, L. Pogany
Colleagues... This is a broad enough question that it can have many answers. Would any of you like to help me answer this for this student?
I would think answers to both the list and to this student would be useful since he is not a member of the Server.
Nestor Your Friendly Neighborhood SysOp -----------------------------------------------------------------------
Email: pjrandsjr-at-worldnet.att.net Name: Philip Randolph School: Gonzaga Preparatory School
Question: We are looking into the development of the electron microscope as a turning point in history. What specific information can you provide that will give us a sense of the effect of the advent of the electron microscope on the cultural, social and economic environment of society? Thank you for your time in considering this question.
} We are in the market for a small film drying cabinet for our EM film. We } don't need, or have space for one of thoses locker-sized jobs they sell in } photo suplly catalogs. Any ideas on where to look? Or better still, is } anyone looking to get rid of one? }
Dear Lee, In the darkroom where we don't have a wall-mounted film dryer, we
use a lab oven at 45 C. The wall-mounted dryers are about 36" high, 24" wide, and 18" deep. They fit nicely over the sink. My personal preference is to air-dry the film overnight; there's less spotting and streaking that way. Yours, Bill Tivol
ELECTRON MICROSCOPY SPECIALIST (Materials Science) New Mexico State University
The Electron Microscopy Laboratory at New Mexico State University is seeking an Electron Microscopy Specialist in Materials Science area. The laboratory provides transmission and scanning electron microscopy and some light microscopy services for the university research community and a few external organizations, in biological, physical and materials science fields.
Qualifications: Bachelors degree in a physical science or materials engineering area (in hand by hire date). Masters degree desirable. Candidates must have at least three years of related experience in scanning electron microscopy and energy-dispersive X-ray microanalysis. TEM experience would be a plus. A working knowledge of Windows based PC computers is essential. Experience with digital image capture and analysis, fluorescence microscopy is desirable. The candidate must be familiar with materials sample preparation techniques, vacuum evaporation, sputter coating, mechanical and electronic equipment, and vacuum systems. The successful candidate must be able to work well with researchers, staff, and students and be able to train graduate and undergraduate students for independent work with relevant techniques and equipment. The candidate should be able to work independently, plan and organize multiple projects and willing to learn new techniques.
Duties and Responsibilities: Operation and routine maintenance of transmission and scanning electron microscopes and associated equipment; supervision of facility users; record keeping, including billings, budgets, toubleshooting and maintenance of instrument and research logs, specimen preparation, data generation and some analysis.
Screening of applicants will begin January 4, 2000 and continue until a candidate is chosen.
Applications should include a resume, letter of application and three letters of recommendation. Position contingent upon funding. NMSU is an EEO/AA Employer.
Apply to: Dr. Reed Dasenbrock Associate Dean/Director Arts & Sciences Research Center New Mexico State University MSC RC, Box 30001 Las Cruces, NM 88003 cgower-at-nmsu.edu
Soumitra Ghoshroy Ph.D. Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003 Phone: 505-646-3600/1531 Fax: 505-646-5665 E-mail:ghoshroy-at-nmsu.edu
The LabSource catalog has a chemical resistance chart for latex and nitrile gloves and about a dozen pages of latex, nitrile and vinyl gloves for sale. I have no affiliation with this company. Bill Tivol
If yes, could that person identify her/himself so that these messages can be directed directly there?
If not, I think it is pretty evident that the majority is for keeping one list and I think we can safely stop this thread and use the bandwidth for other information.
(Please don't start another thread now about whether we can stop this or not!!!!)
Just my humble opinion....
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: Witold Zielinski[SMTP:WIZIEL-at-INMAT.PW.EDU.PL] } Sent: Thursday, December 09, 1999 5:52:45 AM } To: Microscopy-at-sparc5.microscopy.com; "jcoleman-at-rdg.boehringer-ingelheim.com"-at-sparc5.microscopy.com } Subject: Re: One List } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Since it seems that it is a kind of voting - I vote to keep one list (despite that I am interested with metals and crystals). Cheers, Witold Z.
One List yes. But might I suggest using a * or # symbol preface. Thus prefacing the Subject with a *B/ for biological interest, *M/ for Materials. No prefacing of the Subject would indicate universal interest. Most E-mail filters should be able to recognize *B/, *M/ in the Subject heading and filter the E-mail out or into separate folders or mailboxes. For those who forget to use the */ preface or simply don't want to be bothered, the message will be available to all. Cheers, Henry
Henry Eichelberger, Manager Electron Microscopy Facility Department of Biological Sciences Binghamton University Binghamton, NY 13902-6000
Scanning Electron Microscopist Northwestern University
The electron probe instrumentation center (EPIC) at Northwestern University has an immediate opening for a scanning electron microscopist. EPIC is a part of the world renowned materials research center (MRC) and the department of Materials Science & Engineering at Northwestern.
The scanning electron microscopist would be in charge of all of EPIC SEMs (Hitachi S570, FE SEM S4500 and VP SEM 3500N), their accessories (EDS, EBSD/OIM, LHe stage; systems) and the Hitachi FB-2000A focused ion beam (FIB) system. All microscopes in EPIC are under full service contract. Thus, the duties include mainly training students/users, development of specialized techniques and applications, minor maintenance, record keeping and billing. A BS/MS degree in physical/biological sciences is required. The person must have hands-on experience in all aspects of SEM: specimen preparation, EDS, digital acquisition, processing etc. All levels of experience will be considered. Compensation would commensurates with experience and qualifications.
Send cover letter, resume and three references to:
Prof. Vinayak P. Dravid, Director EPIC Materials Science & Engineering Northwestern University, 3013A MLSB Evanston, IL 60208 E-mail: v-dravid-at-nwu.edu Fax: (847) 491-7820
http://epic.ms.nwu.edu/epic/index.htm
Northwestern University is an Affirmative Action/Equal Opportunity Employer.
Hiring is contingent upon eligibility to work in the United States.
************************************************ Wen-An Chiou Department of Material Science and Engineering Northwestern University 2225 N. Campus Dr. Evanston, IL 60091
I think one list is the way to go. I am a biological person, but am learning a lot from the materials postings.
Soumitra Ghoshroy Ph.D. Electron Microscopy Lab Box 3EML New Mexico State University Las Cruces, NM 88003-8001 Tel: 505-646-1531/3600 Fax: 505-646-5665 e-mail: ghoshroy-at-nmsu.edu
} Colleagues... This is a broad enough question that it can have many } answers. Would any of you like to help me answer this for this student? } } I would think answers to both the list and to this student would be useful } since he is not a member of the Server. } } Nestor } Your Friendly Neighborhood SysOp } ----------------------------------------------------------------------- } } Email: pjrandsjr-at-worldnet.att.net } Name: Philip Randolph } School: Gonzaga Preparatory School } } Question: We are looking into the development of the electron microscope as } a turning point in history. What specific information can you provide that } will give us a sense of the effect of the advent of the electron microscope } on the cultural, social and economic environment of society? Thank you for } your time in considering this question.
They might want to look at "Picture Control, The Electron Microscope and the Transformation of Biology in America, 1940-1960", by Nicholas Rasmussen. Stanford Univ. Press, 1997. It got a good review in Science a while ago.
Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
I would also vote for one list. I would suggest that the subject should begin with one or two letters in parentheses: (B) = Biological. (P) = Physical. (BP) Of possible general interest to microscopists.
Regards, Kent
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A. Kent Christensen, Professor Emeritus Department of Cell and Developmental Biology, Medical Science II Bldg. University of Michigan Medical School, Ann Arbor, MI 48109-0616 Office: Room 2703A Med. Sci. II Bldg. E-mail: akc-at-umich.edu, Tel. (work) (734) 763-1287, Fax. (work) (734) 763-1166 http://www.umich.edu/~akc/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
I used to have a bookmarked website for an archive of biologically related messages to the list that I think was maintained by someone at U. of South FL. The listings were organized by subject matter, which made the resource very handy. Unfortunately, I believe the bookmark got lost during a recent computer installation and software reload. If anyone has any information on an archive like this, would you please send me the address?
Many thanks, Missy Josephson
Eleanor Josephson, DVM, PhD University of Connecticut Health Center Department of Anatomy MC-3405 263 Farmington Ave. Farmington, CT 06030-3405 Ph.(860)679-2463 Fax (860)679-1274 ejosephs-at-neuron.uchc.edu
This is the address of the lab page. Tips and Tricks is the top option on the left.
http://www.biotech.ufl.edu/~emcl/
At 04:50 PM 12/9/1999 -0500, you wrote:
} I used to have a bookmarked website for an archive of biologically related } messages to the list that I think was maintained by someone at U. of South } FL. The listings were organized by subject matter, which made the resource } very handy. Unfortunately, I believe the bookmark got lost during a recent } computer installation and software reload. If anyone has any information on } an archive like this, would you please send me the address? } } Many thanks, } Missy Josephson
As the name implies, Phase Contrast Microscopy detects differences in phase (refractive index and/or thickness) between components in a system. In order for this type of microscopy to work, however, you need very small phase differences: small enough so that the light interacting with the sample is slowed down or put out of step with the surrounding background light by only a quarter of a wavelength. This system works really well with things like cells but I would have serious concerns with a very 3D specimen like your embryo. Other techniques which detect phase GRADIENTS (DIC and Hoffman Modulation Contrast) would work much better.
For a further discussion of how each of these techniques operates, might I suggest "Optimizing Light Microscopy"? Details of the book are on our website.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 02:22 PM 12/9/99 -0500, David A. Cantor wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Philips Analytical put out a special issue of Electron Optics Bulletin entitled "The Contribution of Electron Microscopy to Society" This issue was from the Proceedings of a symposium on the topic of the same title in Eindhoven in 1987 (dated, I know).
If you can obtain a copy of this it might be a good starting point. I would try contacting Philips
Cheers Paul
______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
Philips Analytical put out a special issue of Electron Optics Bulletin entitled "The Contribution of Electron Microscopy to Society" This issue was from the Proceedings of a symposium on the topic of the same title in Eindhoven in 1987 (dated, I know).
If you can obtain a copy of this it might be a good starting point. I would try contacting Philips
Cheers Paul
______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
My university runs a Education Center for the Gifted in Science and I am involved in a mentorship program of the school. I teach three middle school boys and girls in my group. We decided to do a project of studying 'clam shell' with various analytical tools including SEM, XRD, and XRF. We have already collected information on the amount of clam and oyster shell production in my country.
Now we need some information on the science of clam shell as a reference, such as the structure, mechanism of formation, function, composition, or anything.
Do any of you have been in the field of clam or clam shell? Do any of you know good references on the clamshell? Any information would be very helpful to me and youngsters.
Jondo Yun Department of Inorganic Materials Engineering Center for Instrumental Analysis Kyungnam University 449 Weolyeong-dong, Masan, 631-701, Korea 82-551-249-2697 (tel) 82-551-248-5033 (fax) jdyun-at-hanma.kyungnam.ac.kr
} what are the advantages of a phase contrast microscope over a } conventional light microscope and why would i use one to examine } embryos
Very simply, without going into details of the principles of the technique, because it allows you to view unstained specimens.
Regards
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
We are probably removing a Philips EM 300 transmission electron microscope early next year in a departmenal reorganisation. Until this year it was maintained by Philips, and currently by a third party. It runs as well as when it was installed in April 1971.
Any offers would naturally be welcome, failing that, if anyone wants to remove it, they probably can. We need the room. Maybe someone would like parts for spares? Are there any more still running out there? This one is 28 years old. It runs well at 80 kV but we have never used it routinely at 100 kV, although it passes service muster at 100 kV. It has the high resolution and goniometer stages. There is a rotating specimen holder for the latter. The mercury diffusion pump was removed some years ago. We will regret the passing of this trusty instrument!
Regards - Keith Ryan
_______________________________ Dr. Keith Ryan Marine Biological Association of the UK The Laboratory Citadel Hill Plymouth Devon PL1 2PB England
Tel. ++44 (0)1752 633249 Tel. ++44 (0)1752 633279 The 279 number has an answering machine
Scott, Try Philips service at 1-800-432-1734. I have a 20+y/o Haskris and Philips has put a new filter on it during a pm on my EM201. Bob Santoianni Emory University Hospital Atlanta, GA
Does this list have a policy (written or otherwise) about vendor postings? I have read the "welcome" message I got way back in the dark ages, and it seems those rules prohibit direct advertisements. Certain postings I've seen would seem to violate that rule (in spirit at least) in that the signature lines identify the sender as an employee of organization X (as required by the guidelines) which provides service Y (the advert). Does this not qualify as an advertisement? Without a doubt, it is free air time for the vendors involved. In certain cases, the violation has been less discrete - when posters have requested information on a product/service, vendor X replies on-list even though the poster's address is there for a discrete reply. I'm just interested in where the line would be drawn (if there is one).
Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-496-2088 fax 301-402-0396
You may want to check Haskris itself at www.haskris.com, we have found them to be good. It is also worth mentioning that their coolers are so common that our University Physical Plant knows how to service them.
++++++++++++++++++++++++++++++++++++++++++++++++ Laurence Marks Department of Materials Science and Engineering Northwestern University fax: (847) 491-7820 mailto:l-marks-at-nwu.edu http://www.numis.nwu.edu ++++++++++++++++++++++++++++++++++++++++++++++++
I do monitor (but not edit) the submissions. When I see that a posting has crossed that grey line which loosely defines (the spirit) of our rules of conduct then I contact the person(s) involved. Since I do not always read every message I sometimes miss some transgressions, but people are pretty good about this and I get off-line messages when things appear to be, shall we say, in violation of conduct.
As a general rule most vendors are VERY VERY good and do not post advertising. I do get complaints from Company Y about Company X and usually after review a solution is found. The most common problems are experienced with "new" users who do not yet have experience in this forum and a gentile reminder is sufficient. There are also the grudge matches which happen and then I have to play mediator a job which is no fun at all. (Hmm... maybe I should ask for a raise?).
Anyway, to speak to the question which Bob Plamor raised, the rules of conduct (look at the WWW SIte the URL is on EVERY Email message) do permit vendors to say they make a product which can solve a particuliar problem. They are asked to be concise and to the point and then contact the individual off line. Vendors are one of our resources in my mind. Yes they do sell things and as long as the reply answers a question which was posted on the server this is valuable information and totally within the spirit of the server. Sometimes they do get verbose and when I notice this happening I let them know privately.
All in all, the vendors are important to us. They read closely what is going on and the discussions surely influence what they think of the scientific market place and what people need/want to conduct their research. They are not just simply someone to be put up with they are our colleagues abeit with different driving forces. Some do only have only commerical motivations, but I'm sure you will agree that there are those that have a passion for the science as well and are there also to help us.
You should also remember that this is not a closed list and occassionally spam/junk mail gets through the filter. These are completely off topic and very hard to manage unless we change to a completely closed list. That is something which I would like to avoid as it not only means additional work, but makes us less accessible.
The venerable coolwell S100 chiller attached to our TEM responded to a recent move from one building to another by dying. Does anyone have schematics, or even an operator's manual for this thing. The guys from physical plant and I need a starting place..... TIA Julian
Julian P.S. Smith III Dept. of Biology Winthrop University Rock Hill, SC 29733 803-323-2111 x6427 (vox) 803-323-3448 (fax)
Soumitra Ghoshroy Ph.D. Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003 Phone: 505-646-3600/1531 Fax: 505-646-5665 E-mail:ghoshroy-at-nmsu.edu
Yours is a very insightful question. My question is, are vendors bad guys? Would you prefer to not have easy access to instrumentation and technology?
I have been a member of MSA for about 20 years and now, as a vendor, am delighted to be a sustaining member. Vendors, do not, in my opinion get any "free air time." We pay for everything we get.
Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. PMB-499, 1807 West Slaughter Lane, Number 200 Austin, Texas 78748 Phone: 512/282-5507 FAX 512/280-0702
Quality Electron Microscope Repair
-----Original Message----- } From: Robert Palmer {rjpalmer-at-dir.nidcr.nih.gov} To: Microscopy-at-Sparc5.Microscopy.Com {Microscopy-at-Sparc5.Microscopy.Com}
Sorry to extend this thread at all, but I just feel compelled to remind any of those who wish to separate/segregate the list - It is your combined presence on this listserver that make it so absolutely functional! Our depth is tremendous. Sure I can choose to ignore *Bio messages, or I could refrain from signing up for the Bio sector of the listserver, if we were to separate. So then I could focus on the work at hand. Not troubled by the problems of those "Bio" guys trying to do that impossible staining or labeling... But, then how would I ever know that some "bio-microscopists" was doing things that may work for me. Or worse yet, how would I know that some "bio-microscopist" could use my help and my recipe for xyz to embed his zyx.
Yes we may have our dysfunctional moments, like any good family unit must, just to maintain good perspective. (by the way, This is one of those dysfunctional moments.) Please do not consider diminishing this powerful resource. And Please Stay Tuned - to the whole picture. Brad Huggins
Thank you to all that replied. The contact information for Haskris is
80 W. Seegers Rd. Arlington Heights, IL 6005 Ph: 847-956-6420 fax: 847-956-6595
The strainers are $13 ea and yes they do take AMEX cards. Minimum order is $35.
-Scott
} -----Original Message----- } From: Walck. Scott D. [mailto:gls4590-at-smtpgate.go.ppg.com] } Sent: Thursday, December 09, 1999 1:18 PM } To: 'Microscopy' } Subject: filters for Haskris water chillers } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------. } } } Does anyone know where I can get the filters for a Haskaris } water chiller } where I can use an AMEX card? } -Scott } } } Scott D. Walck, Ph.D. } PPG Industries, Inc. } Glass Technology Center } Guys Run Rd. (packages) } P. O. Box 11472 (letters) } Pittsburgh, PA 15238-0472 } } Walck-at-PPG.com } } (412) 820-8651 (office) } (412) 820-8161 (fax) } } }
For the 50th meeting of EMSA in Boston (1992) Sterling Newberry wrote a wonderful book entitled "EMSA and Its People - The First Fifty Years". It carries the Library of Congress number 92-72571. Sterling, along with his wife Mary Lou, spent hundreds of hours interviewing many of the Charter Members of our organization. Most of these interviews were video taped by Sterling and then compiled into an "oral history" by Maurice Dumais. Both Sterling's book and Maurice's "oral history" may still be available from the MSA business office or borrowed from an attendee of the '92 Boston meeting. In addition, John Reisner's series of "Reflection" columns in many of the MSA Bulletins talk about the early pioneers and their contributions.
/rich shuman
-----Original Message----- } From: "pjrandsjr-at-worldnet.att.net"-at-Sparc5.Microscopy.Com [mailto:"pjrandsjr-at-worldnet.att.net"-at-Sparc5.Microscopy.Com] Sent: Thursday, December 09, 1999 9:06 AM To: Microscopy-at-Sparc5.Microscopy.Com
Colleagues... This is a broad enough question that it can have many answers. Would any of you like to help me answer this for this student?
I would think answers to both the list and to this student would be useful since he is not a member of the Server.
Nestor Your Friendly Neighborhood SysOp -----------------------------------------------------------------------
Email: pjrandsjr-at-worldnet.att.net Name: Philip Randolph School: Gonzaga Preparatory School
Question: We are looking into the development of the electron microscope as a turning point in history. What specific information can you provide that will give us a sense of the effect of the advent of the electron microscope on the cultural, social and economic environment of society? Thank you for your time in considering this question.
As one of those vendors (and a former 'non-vendor') on the list, I would like to say a few words to this. I am not speaking for any other vendor on the list, this is my own opinion.
I think, the entire issue of vendors of this list is:
a) a gray area and will always stay that way b) something that is handled very well by Nestor.
Personally, when I read a listing I feel the urge to respond to, I make the following decision: Does the posting I intend to put on the list include information that may interest more people than the person whose message I am replying to?
If the answer is Yes, I go to the list. If the answer is No, I respond through email.
An example:
If somebody posts: "Are there ways to reduce noise in digital TEM images?", I'd post on the list. If the question is: "Why doesn't function X work with software Y on my TEM Z", I'd stick with email. Of course these are judgment calls and some people may have broader or narrower definitions for putting something on the list.
I don't see the point, however, to hide my affiliation. I work for a company and not for a University, but why would that disqualify me from making contributions to the list? If you are concerned about more blatant misuse of the list, I don't think there are many, and I am sure that Nestor would deal with those. On the other hand I might mention my company or one of its products in the posting, but there is not much I can do about it: I cannot speak for other vendors so I have to stick to what I can use, which is my own products. In the example above: I can use examples from our own software, but not from any other as I don't want to misrepresent the other software.
One thing that many vendors put in are disclaimers at the end (I must admit that I have forgotten a few times) to make sure that everybody knows that they are interested in selling something. Whether that is useful I don't know. I guess one could filter for "disclaimer" and throw those out. On the other hand exactly that could be interpreted as an advertising.
But what, for example, about conference announcements. Conferences are a business and if you exclude all commercial listings, you would have to exclude them as well. I think, that would be disservice to the community.
In a nutshell: I don't think this issue is a Yes/No issue but rather an issue of balance between usefulness and abuse. Of course I am biased, but I think Nestor (or perhaps the vendors?) do a good job at keeping this ratio at a good level.
but then again... this is only my opinion. (Does this posting count as covert advertising???)
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
******************************************************* Disclaimer: Soft Imaging System produces and sells image acquisition and processing systems. We therefore have a vested interest in some of the items mentioned above. *******************************************************
} ---------- } From: Robert Palmer[SMTP:RJPALMER-at-DIR.NIDCR.NIH.GOV] } Sent: Friday, December 10, 1999 5:59:16 AM } To: Microscopy-at-sparc5.microscopy.com } Subject: vendors } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Does this list have a policy (written or otherwise) about vendor postings? I have read the "welcome" message I got way back in the dark ages, and it seems those rules prohibit direct advertisements. Certain postings I've seen would seem to violate that rule (in spirit at least) in that the signature lines identify the sender as an employee of organization X (as required by the guidelines) which provides service Y (the advert). Does this not qualify as an advertisement? Without a doubt, it is free air time for the vendors involved. In certain cases, the violation has been less discrete - when posters have requested information on a product/service, vendor X replies on-list even though the poster's address is there for a discrete reply. I'm just interested in where the line would be drawn (if there is one).
Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-496-2088 fax 301-402-0396
Hello, I have seen a talk by P. K. Hansma on the study they did of abalone shells.
To paraphrase him: Seashells are interesting as they are 97% CaCO3, 3% organic material; and are 3000x more fracture resistant than a single crystal of CaCO3. =20 Industry is currently doing research on using seashell like material to replace plastics and wood since it would be fire resistant and non-toxic. = =20 Since seashells grow slowly, industry needs to grow them faster.
Studies by SEM showed abalone mother of pearl to consist of stacks of plates of CaCO3. (excuse my poor diagram)
Crack energy is dissipated by the layers stacked randomly. Composites of CaCO3 materials manufactured similar to above didn't work. The reason for the strength of the abalone shell was found to be due to a protein binding between the tablets (Lustrin A). Immunogold labeled SEM showed the material to appear as strands between plates: (again, excuse my diagram)
Pulling CaCO3 plates apart in the seashell causes the protein structure to unfold and many intermediate strength protecting bonds are broken slowly. Relaxing the force causes the protein bonds to reform.
Some useful papers may be: Zaremba, CM; Morse, DE; Mann, S; Hansma, PK; and others. Aragonite-hydroxyapatite conversion in gastropod (abalone) nacre. CHEMISTRY OF MATERIALS, 1998 DEC, V10 N12:3813-3824.
10. Schaffer, TE; IonescuZanetti, C; Proksch, R; Fritz, M; and others. Does abalone nacre form heteroepitaxial nucleation or by growth through mineral bridges? (vol 9, pg 1731, 1997) CHEMISTRY OF MATERIALS, 1998 MAR, V10 N3:946-946. Pub type: Correction/Addition.
12. Shen, XY; Belcher, AM; Hansma, PK; Stucky, GD; and others. Molecular cloning and characterization of lustrin A, a matrix protein from shell and pearl nacre of Haliotis rufescens. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997 DEC 19, V272 N51:32472-32481.
18. Walters, DA; Smith, BL; Belcher, AM; Paloczi, GT; and others. Modification of calcite crystal growth by abalone shell proteins: An atomic force microscope study. BIOPHYSICAL JOURNAL, 1997 MAR, V72 N3:14= 25-1433.
26. ZAREMBA CM; BELCHER AM; FRITZ M; LI YL; and others. CRITICAL TRANSITIONS IN THE BIOFABRICATION OF ABALONE SHELLS AND FLAT PEARLS. CHEMISTRY OF MATERIALS, 1996 MAR, V8 N3:679-690. =20 1. Shen, XY; Belcher, AM; Hansma, PK; Stucky, GD; and others. Molecular cloning and characterization of lustrin A, a matrix protein= =20 from shell and pearl nacre of Haliotis rufescens. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997 DEC 19, V272 N51:32472-32481.
22. Taylor, John David. The shell structure and mineralogy of the Bivalvia, by John David Taylor, William James Kennedy [and] Anthony Hall. London, British Museum (Natural History), 1969-73. Series title: Bulletin of the British Museum (Natural History). Zoology. Supplement ; 3. Series title: British Museum (Natural History) Bulletin. Zoology, v. 22, no. 9.
I hope this information may be of some use to you. The formation of seashells is very interesting chemically since it involves chemical precipitation aided by an organic biological template. Often crystal forms predicted to be unstable thermodynamically are precipitated due to the effect of the organism and the biological material it produces.
I am interested in researching sea shells myself, but haven't had the opportunity to work with anyone. I have acess to TEM, SEM, and AFM instruments, so if someone is interested in working together, drop me a line.=20
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\= /\ Gordon Ante Vrdoljak 1 Cyclotron Road ICQ 23243541 http://nature.berkeley.edu/~gvrdolja MS90-1116 GAVrdoljak-at-lbl.gov Ernest Orlando phone (510) 495-2829 Lawrence Berkeley fax (510) 486-7797 National Laboratory cell (510) 290-6793 Berkeley CA 94720
On Fri, 10 Dec 1999, =C0=B1=C1=B8=B5=B5 wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America= =20 } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } =20 } =20 } Dear members: } =20 } My university runs a Education Center for the Gifted in Science and I = am } involved in a mentorship program of the school. I teach three middle sch= ool } boys and girls in my group. We decided to do a project of studying 'clam } shell' with various analytical tools including SEM, XRD, and XRF. We have } already collected information on the amount of clam and oyster shell } production in my country. } =20 } Now we need some information on the science of clam shell as a referenc= e, } such as the structure, mechanism of formation, function, composition, or } anything. } =20 } Do any of you have been in the field of clam or clam shell? } Do any of you know good references on the clamshell? } Any information would be very helpful to me and youngsters. } =20 } Jondo Yun } Department of Inorganic Materials Engineering } Center for Instrumental Analysis } Kyungnam University } 449 Weolyeong-dong, Masan, 631-701, Korea } 82-551-249-2697 (tel) } 82-551-248-5033 (fax) } jdyun-at-hanma.kyungnam.ac.kr } =20 } =20 } =20 } =20
I notice that you have not included light microscopy in your list. We typically recommend, especially in this sort of case, that your students start with a simple hand lens, then move to a stereo microscope ... both of which will give a lot of information about context and macro structures. They should then move to the compound microscope, looking at both the inner and outer surface of the shell. Polarized light will give some amazing information about the orientation of the calcium carbonate and how it was layed down when the shell was formed. None of this information is available with the other techniques cited.. and I wouldn't want either you or your students to miss out on this part of the "mystery".
Good hunting and best regards,
Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 12:21 PM 12/10/99 +0900, =C0=B1=C1=B8=B5=B5W1 wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20 school } boys and girls in my group. We decided to do a project of studying 'clam } shell' with various analytical tools including SEM, XRD, and XRF. We have } already collected information on the amount of clam and oyster shell } production in my country. } } Now we need some information on the science of clam shell as a reference, } such as the structure, mechanism of formation, function, composition, or } anything. } } Do any of you have been in the field of clam or clam shell? } Do any of you know good references on the clamshell? } Any information would be very helpful to me and youngsters. } } Jondo Yun } Department of Inorganic Materials Engineering } Center for Instrumental Analysis } Kyungnam University } 449 Weolyeong-dong, Masan, 631-701, Korea } 82-551-249-2697 (tel) } 82-551-248-5033 (fax) } jdyun-at-hanma.kyungnam.ac.kr } } } } }
Just another interesting note on EM in history. The Arts & Entertainment channel on the tube did a "100 greatest achievements of the Century" and Electron Microscopy was #59 (or somewhere close to that). Right up there with tungsten lighting and insulin. Perhaps the student could check with the producers of that program.
} } } } } Keep it to one list { { { { { { { { {
David O'Neil National Research Council of Canada Institute for Marine Biosciences 1411 Oxford Street Halifax, NS B3H 3Z1 ph: (902)426-8258 fax: (902)426-9413 e-mail: david.o'neil-at-nrc.ca
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Hi, I have a researcher who plans to embed developing zebra fish to study development of the inner ear. Samples will be fixed and embedded up to age 30 days. We are not concerned about early stages but did not know when calcification starts and if this would be a problem for the late stages.
Has anyone had experience with fish or other embryos at the early stages of calcification? Did you need to do any decalcification and, if so, what procedure did you use? We are not interested in the bones themselves but the soft tissue and are concerned that calcified skull could cause tearing of the nearby tissues when the block is sectioned. Also any special protocols for optinmum fixation?
Thanks in advance for your help. Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
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Dear all
I need to do special staining for giant mitochondria in transplant kidney bx, it's chromotrope aniline blue. The ref. I found was incomplete. I will be very happy if anyone coul send me the formula. If you also have the formula of Sirius red for interstitial fibrosis, my happiness will be complete.
We have had an R75 and an R100 for many years now. I bought them based on Philips' recommendation. We have had to replace the water pump on both units about once a year. According to Haskris, that's too frequent. What happens is that the brass coupling between the pump and the motor wears unevenly. We run it until the pump fails. Haskris suggested installing a new coupling every 6 months as routine p.m. There has been one occasion where the pump has failed in 5 months. The coupling was worn down to 20% of its mass. Has anyone else experienced this?
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Dear All,
Give these guys a break! Where would we be without vendors?
Not only are they the source of ALL our equipment but in many instances = are also a very valuable source of information. They know the equipment = better than most, can identify trends faster, do visit all the labs = working with microscopes so can see new technologies before we do, and can = provide solutions to problems that may seem impossible when viewed up = close. =
I think the moderation of the list is tough enough, both from the = contributors and from Nestor, to ensure that what gets through is tasteful = and concise. I encourage vendors to continue contributing not only = because they are at the front of what is happening, but also because many = are established and experienced microscopists too. When your reps come = through again, try talking with them. You might learn a thing or two.
Regards, =
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 phone:213 273 8026 fax: 213 413 6739 e-mail: pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
PS. On the subject of splitting the list - As a biologist I think we have = lots to learn from the materials people, especially when it comes to = sectioning. Having talked with material scientists, I think there are one = or two things we can pass on too. Sorry Lorry, but my vote is to stick = with one list.
Finally: A note to Nestor - I like the idea of you giving out "gentile = reminders" but I need to know if jewish ones are more or less tough?
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I would appriciate it, if you would subscribe me to the microscopy list sever.
Thanks,
Christian Kuebel
------------------------------------------------- Dr. Christian Kuebel University of Michigan Department of Materials Science and Engineering 2125 H. H. Dow Building 2300 Hayward Street Ann Arbor, MI 48109-2136 Phone: (734) 763-4196
I purchased a Nachet 300 tricoular with what appears to be a phase condenser, G.C. 10x eyepieces a P8x in the camera tube, oculars: 60 1.00, 40 .65, 20 .55 and 10 .27. The thing that I don't understand is there are two dove tail sliders abover the oculars that can be inserted or removed from the light path and one has a very fine adjustment in the light path.
I would like a manual in English but I doubt one is available. I will go by the library and find a book on phase scopes to learn the adjustememt procecure.
It seems like a well built scope except the 35 mm camera that came with it looks like it came from a box of cracker jacks. I know that all the camera is is a box that holds film but this is the a sorry excuese for a 35 mm back. That is not much of a concern as I will be replacing it with a video camera.
Can some on tell me what the sliders are above the objectives. If it were a poloriszed scope they would be phase plates. They don't give enough color to be phase plates unless the polorizer is missing.
Thanks Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger 405 624-2855 GMT -6:00 www.couger.com/gcouger
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We have had one Haskris R100 with two pumping units for about 30 years. We just replaced the pump on one unit which, I believe, is only the second time it has been replaced. The other one has also been replaced at least once as well. The original motors were on the unit although the compressor was replaced once and the copper piping has been replaced/repaired over the years. Another double pump R100 unit has had similar types and frequency of repairs.
Our compressors have gone out primarily beause they are water cooled and were on hard water. Mineral build-up restricted the cooling water causing failure. Now our maintenance crew runs dilute acid through them yearly to prevent this problem.All work is done by our in-house plumbing and refrigeration crew. Debby
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057
Vachik Hacopian wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Rick Felten-at-MACDERMID 12/11/99 12:49 PM I specialise in using the SEM to characterize bulk surfaces, but as a prelude to the analysis of each sample I always preview it w/ a Nikon low mag stereo inspection microscope. This light microscope often gives me the info I seek, and I simple use the SEM to confirm or prove my expectations. I am not knowledgeable about light microscopy, but when I compare the information I get from my Nikon (20-30 years old) to many other stereoscopes, I find that all the others at my company give very poor performance. I want to purchase a stereo microscope for my kids to grow up with. Something that matches our visual world but simply magnified To me this means excellent resolution, brightness, and 3-D. I was wondering who are the "undisputed" quality vendors and who are the vendors that produce a fine product, but don't have a name like Carl Zeise, Nikon and Leica. How much does one have to spend to get the quality that I am used to in my Nikon. How high in magnification can one achieve and still have good stereo information? I am interested in only reflectance mode work. Has anyone recently purchased a good basic stereo microscope that impressed them. I would like to have the ability to ditigize at a later date, so I assume I need a phototube? I see a ad in Cole palmer for a Meiji microscope about $2000. I have also seen prices that ranged from $300 to $7000, where is a good cut off for price/performance ratio? Ric
We have an immediate need to locate and purchase a suitable water chiller/recirculator in good used condition. The unit must be an air-to-air-type with a cooling capacity of approx 15,350 BTU per hour. Likely models include Haskris R100 or Neslab HX 150. Would prefer a unit geographically nearby.
Please respond directly to:
Bob Roberts EM Lab Services, Inc. 2409 S. Rural Rd Tempe, Arizona 85282 (480) 967-3946
This subject comes up now and then, and please allow me to add my commercially oriented view. The original 'Internet' had usage policies that forbade the conveyance of commercial 'advertising', although many of the participants were large commercial concerns. Nearly a decade ago, the Internet was commercialized, leading to the present situation, such as it is, where the usage rules simply don't apply. This was a result of the turnover of the Internet to commercial, rather than governmental control.
Obviously, there are blatant abusers out there in the nasty world of the Internet. IMHO, one of the worst is AOL, the leading ISP. However, there are also relative bastions of calm such as this list. Primarily due to its esoteric nature, and the efforts of Nestor, this list has remained relatively free of blatant commercialism.
I have never started a thread, but have often responded to one. There have been one or two occasions over the years where our friendly sysop (Nestor) has contacted me regarding the suitability of a posting I made. In each case, I believe we have managed to come to a reasonable mindset where I have not been overtly commercial and the list has not been harmed by my postings.
The bottom line is that academic purity is not damaged by the honest presentation of commercial concerns, and commercial purity need not be compromised by academic concerns. On the contrary, a substantive admission of orientation, both on the commercial and academic sides, can only enhance the interchange.
The subject of signature lines is simply specious. A proper signature line merely identifies one as belonging to one organization or another, and, as such, helps the viewer to determine one's affiliations and potential biases. Whether a university, a company or a high school might benefit from a particular signature line is really up the the viewer. If you think that you should be the arbiter of what each viewer reads in those lines, then I suggest that you might consider therapy.
I believe that most viewers, including myself, can come to reasonable conclusions based on the affiliations presented. This is far preferable to a list where no affiliations are presented and one is left not knowing the source of a particular view. No doubt in your view, this represents an unreasonable black vs. white approach, but the truth is that you either allow affiliations or you don't. The only alternative is to allow affiliations only for a chosen few and that doesn't cut it in the modern world.
In regards to your statement that "Without a doubt, it is free air time for the vendors involved", how many academic postings are made with affiliations noted in the signature line with the intention of aggrandizing an individual, department or organization? Let's be honest, a signature line, whether private or public, is an attempt to tie an individual to an organization that may carry some weight to that individual's view or enh ance the organization's stature should that individual's view be accepted. Academic ventures are surely not immune to financial concerns.
The purpose that all of us should follow is the enhancement of the scientific principles that through the centuries have provided the enlightenment we have pursued. Rather than getting caught up in the micro-management of that process, we should be involved in the active management and stewardship of the principals that have brought us so far.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com
-----Original Message----- } From: Robert Palmer [SMTP:rjpalmer-at-dir.nidcr.nih.gov] Sent: Friday, December 10, 1999 6:59 AM To: Microscopy-at-sparc5.microscopy.com
Dear list members,
I may get involved in the installation of a Cambridge Microscan 9 EPMA donated to an institute in Hungary. I would highly appreciate any kind of information about that model.
Clarence A. Miller Department of Chemical Engineering Rice University 6100 S. Main Street Houston, Texas 77005-1892 Telephone: 713-527-4904 FAX: 713-285-5478
I am working with leukemia cells in suspension and spin them down in conical BEEM capsules prior to fixation. All subsequent steps (osmication, dehydration, infiltration with epon 812) are performed in these tubes. Unfortunately the blocks are very difficult to cut. The tips are with holes and inside the blocks there are "craters" and spongy areas that make it very difficult to cut proper sections. The pellets are minute, therefore I like the convenience of the conical tubes but I have to cut "big" chunks away with the razor blade before I get reasonable sections which makes it a quite wasteful job. The absolute EtOH is dried over Cu2SO4 in a dialysis tube and the cells are in filtrated twice within 24 hours.
I know that one way out of this dilemma would be to handle the pellet as a tissue - but the experiment is so time consuming that I still do not want to risk having a crumbling pellet after 2 days work.
Thanks in advance for your kind help.
Claudia
Dr. C. Hayward-Costa School of Life Sciences Kingston University Penrhyn Road, Kingston upon Thames Surrey KT1 2EE, UK ++44(0)181 547 2000 x 2240 Email: c.hayward-at-kingston.ac.uk
} } I recommend Dr. Cheng Huang {chuang-at-ccs.carleton.ca} at Carleton University, } Ottawa, Canada. } } John Catino } } } } } "Tian_Huang-at-gillette.com"-at-sparc5.microscopy.com wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } Hi, there, } } Could anybody tell me where I can get cryo-SEM done? Thanks! } } } } } } Tian }
Greetings, I use a PowerPoint presentation to teach farmers, county extension agents and horticulturalists about basic soil science. I need a scanning electron microscope image of kaolinite. Only one or two very good images are all that I need. Can anyone help with this? I thought there might be a national library of SEM images for public access but there doesn't seem to be one. If you can help, please email me privately at: blpprt-at-clemson.edu Thanks! Bob Lippert
********************************************************* Dr. Bob Lippert Dept. Crop and Soil Environmental Science 277 Poole Agricultural Center Box 340359 Clemson University Clemson, SC 29634-0359 Phone 864-656-3502 FAX 864-656-3443 EMAIL blpprt-at-clemson.edu WEB SITE: http://hubcap.clemson.edu/~blpprt/bobweb/bobweb.html
Soumitra Ghoshroy Ph.D. Electron Microscope Laboratory Box 3EML New Mexico State University Las Cruces, NM 88003 Phone: 505-646-3600/1531 Fax: 505-646-5665 E-mail:ghoshroy-at-nmsu.edu
We are in need of an architect to help us design a TEM room in a stand alone structure. The building will be an empty shell, so we can do pretty much as we please. The FETEM is used for high res work which means it has some very special needs. If you know of a firm in this area {NY, NJ, PA, CN} which can handle this please contact me via e-mail.
I have no experience working with JB-4 resin and a graduate student is trying to embed whole fish (trout) eggs for a histological examination. The eggs are about 5 mm in diameter. So far, penetration into the yolk is incomplete or not at all. Vacuum infiltration is not recommended because it may speed up polymerization of the JB-4 resin and the student would like to have the eggs intact during preparation. Does anyone have any suggestions? Or can one point us to a literature source for this type of work?
Many thanks, Susan ************************************** Susan Belfry Electron Microscopy Unit University of New Brunswick, Bag Service #45111, Fredericton, New Brunswick, E3B 6E1, Canada Phone: 506-453-4887 Fax: 506-453-3583 http://www.unb.ca/emunit/ *************************************
Claudia, I have processed many cell pellets in the 0.6ml microcentrifuge tubes (I have never tried to use the BEEM capsules for processing). I have had no problems processing and embedding the cells all in the same tube. It really sounds like the cells are not thoroughly dehydrated. What kind of dehydration series are you using? I have found that if I use a rinse with 100% acetonitrile after the 100% EtOH and then infiltrate with a 2:1, 1:1, 1:2 acetronitrile:eponate 12 series I have absolutely no problems with the finished block. Also, before putting the pure eponate 12 resin in the tube (the final step before polymerization) I remove every last bit of acetonitrile with a small absorbent cotton swab. This way I am satisfied that there is absolutely no water or other liquid around my cells which may prevent the resin from getting all the way down into the tip of the tube. I hope this works for you! Good luck, Jo Dee
Jo Dee Fish Electron Microscopy Assistant The Burnham Institute 10901 N. Torrey Pines Rd. La Jolla, CA 92037 858-646-3100 ext.3620
Claudia Hayward-Costa wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Experts, } } I am working with leukemia cells in suspension and spin them } down in conical BEEM capsules prior to fixation. All subsequent } steps (osmication, dehydration, infiltration with epon 812) are } performed in these tubes. } Unfortunately the blocks are very difficult to cut. The tips are with } holes and inside the blocks there are "craters" and spongy areas } that make it very difficult to cut proper sections. } The pellets are minute, therefore I like the convenience of the } conical tubes but I have to cut "big" chunks away with the razor } blade before I get reasonable sections which makes it a quite } wasteful job. } The absolute EtOH is dried over Cu2SO4 in a dialysis tube and the } cells are in filtrated twice within 24 hours. } } I know that one way out of this dilemma would be to handle the } pellet as a tissue - but the experiment is so time consuming that I } still do not want to risk having a crumbling pellet after 2 days work. } } Thanks in advance for your kind help. } } Claudia } } Dr. C. Hayward-Costa } School of Life Sciences } Kingston University } Penrhyn Road, Kingston upon Thames } Surrey KT1 2EE, UK } ++44(0)181 547 2000 x 2240 } Email: c.hayward-at-kingston.ac.uk
I am working with HELA and COS7 cells in suspension. I would prefer to fix (GA and OsO4 postfixation) them in suspension in 1.5 ml Eppendorf tubes. Usually I collect cells by centrifugation of the suspension in the standard cell culture 15 ml tubes at the low speed (not more than 1000 rpm on the GLC-2 backet rotor centrifuge, 10-15 min) and resuspend them in the 0.5-1 ml fixer solution. Than I transferred cells suspension into the 1.5 ml Eppendorf tubes and performs all manipulations in those tubes (with some centrifugation between steps to hold precipitate in place if necessary). At such conditions cells do not form a solid precipitate, therefore they are more accessible to the solvents. If it necessary at the last step, I transfer cells into BEEM capsules with pure plastic media, centrifuge it at higher than before speed (say 2000 rpm, 20-30 min) and than polymerize it. I find, BEEM capsules is less suitable for cells processing mostly because a small volume. For approximately 50 um precipitate volume I prefer to use about 500-700 um of the each solvent. It may be important at dehydrating steps when in the small volume it is most likely to "re-hydrate" the solvent (and sample as well) from air's moisture.
Good luck.
Sergey
} Dear Experts, } } I am working with leukemia cells in suspension and spin them } down in conical BEEM capsules prior to fixation. All subsequent } steps (osmication, dehydration, infiltration with epon 812) are } performed in these tubes. } Unfortunately the blocks are very difficult to cut. The tips are with } holes and inside the blocks there are "craters" and spongy areas } that make it very difficult to cut proper sections. } The pellets are minute, therefore I like the convenience of the } conical tubes but I have to cut "big" chunks away with the razor } blade before I get reasonable sections which makes it a quite } wasteful job. } The absolute EtOH is dried over Cu2SO4 in a dialysis tube and the } cells are in filtrated twice within 24 hours. } } I know that one way out of this dilemma would be to handle the } pellet as a tissue - but the experiment is so time consuming that I } still do not want to risk having a crumbling pellet after 2 days work. } } Thanks in advance for your kind help. } } Claudia } } Dr. C. Hayward-Costa } School of Life Sciences } Kingston University } Penrhyn Road, Kingston upon Thames } Surrey KT1 2EE, UK } ++44(0)181 547 2000 x 2240 } Email: c.hayward-at-kingston.ac.uk
--
_________________________________
Sergey Ryazantsev Ph. D. UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
It sounds like you have a problem with residue of fluids in your beem capsules which could be preventing decent impregnation of your pellets. You do not mention the source of your cells, but a few years ago when I worked at Red Cross Children's Hospital in Cape Town, we had easy techniques for both peripheral blood samples and aspirated cells.
Peripheral blood was centrifuged in capillary tubes to separate the cells (this was done by our haem lab before they sent them to us). A score was made on the glass of the cap tubes just below the buffy coat and then by placing the broken end into a vial of fixative (we used cacodylate buffered 1.5% gluteraldehyde) and tapping gently on the other end, the plug of cells were released. They were fixed for at least 2 hours and then processed (whole, if 2mm long or less) in an automatic tissue processor (Shandon Lynx) with our routine specimens. I would recommend that if you use an auto processor you should line the basket to stop the specimens from being washed out. We used to use a small piece of lens cleaning tissue with all of our small specimens. These blocks are easy to cut although you sometimes had to trim a bit to get through the layer of platelets as it is nearly impossible to orientate the plugs.
For aspirated cells, we received cells in 1.5% glute which we centrifuged in eppendorf tubes and removed all of the supernatant fluid. We then put a drop of 5% - 10% gelatine or agar (just warmed enough to melt) and slightly loosened the pellet slightly with an orange stick so that the cells were concentrated at the base of the agar plug. This was then set in the fridge and the pellets were trimmed and processed in the tissue processor with the routine specimens. These blocks tended to be slightly soft but they still sectioned perfectly.
Rick Felten-at-MACDERMID 12/14/99 08:04 AM Every few months printers are discussed on the listserver. I was wondering if it wouldn't be a bad idea if we had an annual "printoff". We could have two or three categories ( {$500 and } $500 or what ever). We could have a standard image printed to a standard size. The submittor could record the print time. The standard image could be a composite of features testing resolution, nearly full dark regions, and nearly full white regions. Let me know what you think. Ric
Hi I am trying to set for the first time a passive Voltage Contrast analysis for contact 1, metal 1 and metal 2 using a FIB and I want to know how is it done?, beside the FIB do I need any other equipment's? and is there any publications that I can search about the analysis itself? Thank you for your help.
I usually have no problem with fixation/embedding of cell pellets. However sometimes material is lost during the various steps. I find that if you embed the pellet in agar after fixation that the results are often improved and material is not lost. This will also eliminate the air problems you discribe. I hope that this helps and good luck.
Paula Scott Fish and Wildlife Conservation Commission Florida Marine Research Institute 100 8th Avenue SE St. Petersburg, FL 33701 (727) 896-8626 Fax (727) 823-0166
I am working on the PET/PEN blends and trying to get the microstructure images of this Polymer blends by TEM. However, By Ruthenium staining (Montezinos et al 1985) of ultrathin sections,no phase differences were visible. I espected to obtain the phase seperate of PET phase (domain phase) and PEN phase. I also tried SEM and found no seperation.
Please give any suggestion about "the sample Preparation".
I agree with you that such a side-by-side comparison would be a good idea. Indeed, a few years ago there was such a "Print-Off" that was displayed in the computer lab at the MSA/MAS meeting. Nestor or someone had prepared a test image with several sub-images to test several modes of imaging. I think the image was available via the MSA web server. However, I don't remember seeing one these last couple of years. Maybe it should be an official and permanent part or the annual meeting.
Warren S.
At 08:04 AM 12/14/1999 -0500, you wrote: } Rick Felten-at-MACDERMID } 12/14/99 08:04 AM } Every few months printers are discussed on the listserver. I was wondering } if it wouldn't be a bad idea if we had an annual "printoff". We could have } two or three categories ( {$500 and } $500 or what ever). We could have a } standard image printed to a standard size. The submittor could record the } print time. The standard image could be a composite of features testing } resolution, nearly full dark regions, and nearly full white regions. Let } me know what you think. } Ric
A recent posting reminded me of a problem that infrequently occurs within = our lab. In fully processed specimens, the epon blocks will develop = bubbles as they harden. At times there are so many bubbles that the = specimen will be displaced from an oriented position. To our best = efforts, we have not been able to identify what causes these bubbles. = There does not seem to be a correlation with the age of any one compound = or mold type. However, making up all new components will often (not = always) get rid of the bubbles. We use vacuum, both after mixing epon = components and again before the epon is cured (i.e., with the tissue = embedded in epon). The epon is EMS Embed-812. Processing is on a Lynx, = with glut, cacodylate buffers, osmium, graded alcohols, propylene oxide, = and uncatalyzed epon. Typically, after processing, specimens sit under = vacuum for 2-4 hours in epon with catalyst (BDMA) before being placed in = the oven. Oven temperature is constant (70C), and curing is overnight. I = have always been curious as to what causes these bubbles and how to avoid = their formation.
TIA
P. Steele, Ph.D. Pathology & Lab. Medicine All Children's Hospital
We have recently received a Gatan double tilt heating stage capable of reaching 1000 degC. On our first trial run in the JEOL JEM-3010 a stage mechanical limit alarm started above 550 deg C despite the stage being near to the centre. The stage was free to move in any direction so we do not think it was a real alarm and it slowed down and stopped as the temperature was reduced below 500degC. I was wondering if anyone else out there has had a similar problem?
Secondly someone wants to look at a cross sectional specimen above 500 deg C. Does anyone know of a glue/cement that can be used safely upto 1000 deg C!
Regards
Alan W Nicholls, PhD Manager - Electron Microscopy Service Research Resources Center - East (M/C 337) Room 100 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Dear Claudia, I have processed cultured cells successfully by using polypropylene = centrifuge tubes or microcentrifuge tubes (a little bigger than the BEEM = capsules you have used). I spin after each step and infiltrate in either = Spurr or LRWhite. My last steps are to transfer each infiltrated sample = to the BEEM capsules. Then, I place the BEEM capsules in microcentrifuge = tubes piggyback(you may have to cut the microcentrifuge tubes with cutters = to make them fit) and spin them once more before placing them in the oven. = It's a long prep, but I've had very good results.
Contact alarms usually detect current flow between the sample rod and the rest of the microscope. Could you be getting sufficient thermal electron emission (perhaps from the heating wires) to trip the sensor?
Cheers, Henk
At 02:42 PM 12/14/99 -0600, Alan W. Nicholls wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://web.ceof.ohio-state.edu Murphy's Law: "If anything can go wrong, it will." Commentary: "Murphy was an optimist."
} } Rick Felten-at-MACDERMID } 12/14/99 08:04 AM } Every few months printers are discussed on the listserver. I was wondering } if it wouldn't be a bad idea if we had an annual "printoff". We could have } two or three categories ( {$500 and } $500 or what ever). We could have a } standard image printed to a standard size. The submittor could record the } print time. The standard image could be a composite of features testing } resolution, nearly full dark regions, and nearly full white regions. Let } me know what you think. } Ric
Dee Breger Mgr. SEM/EDX Facility Lamont-Doherty Earth Observatory 61 Route 9W Palisades, NY 10964 USA T: 914/365-8640 F: 914/365-8155 E: micro-at-ldeo.columbia.edu
I am looking for a used ion mill for preparation of TEM samples. If anyone has an ion mill that they are looking to get rid or knows of anyone who is please contact me.
Thanks.
Kathryn Schubel - Assistant Professor of Geology Department of Geology and Environmental Geosciences Lafayette College Easton, PA 18042 U.S.A.
This is directed in particular at any one using an Olympus or Bio-Rad confocal microscope, which I am told save their images in a multi-image TIFF format. If you would contact me directly by e-mail I would appreciate it. I am trying to get my hands on a couple of representative images, contents not important, to see which of the many possible tiff file variants are in use. If anyone else is using multi-tiffs from some other brand of machine, I'd like to know that as well.
Thanks John Russ John_Russ-at-NCSU.edu or DrJohnRuss-at-AOL.com
We have an HM 505 cryostat that may need parts or need to be sent in for repairs. I recall seeing something recently about Microm-Heidelberg equipment being handled by Richard Allan Scientific. Is this true for all HM equipment? We purchased this cryostat through a Zeiss vendor (who no longer handles Zeiss) and we'd really like to make sure that we deal with the proper company.
Who is actually handling repairs and parts for HM cryostats? I'll be contacting Zeiss soon, but in the meantime, I'd like some warning as to what I'll run into.
Please contact me personally,
Jaclynn M. Lett jmlett-at-cid.wustl.edu
Fay and Carl Simon Center for the Biology of Hearing and Deafness Central Institute for the Deaf 818 S. Euclid Ave. St. Louis, MO 63110
Alan, Check out the MRS TEM prep book I, vol 115, p.126, (1988). Eric Fiore and Rodney Herring have a paper in there that describes a way of going up to 1300C for cross sectional samples. The "glue" that they use is Ceramabond 569 from Aremco Products, Inc.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Alan W. Nicholls [mailto:nicholls-at-uic.edu] } Sent: Tuesday, December 14, 1999 3:42 PM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Heating stages } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } We have recently received a Gatan double tilt heating stage capable of } reaching 1000 degC. On our first trial run in the JEOL } JEM-3010 a stage } mechanical limit alarm started above 550 deg C despite the } stage being near } to the centre. The stage was free to move in any direction } so we do not } think it was a real alarm and it slowed down and stopped as } the temperature } was reduced below 500degC. I was wondering if anyone else } out there has } had a similar problem? } } Secondly someone wants to look at a cross sectional specimen } above 500 deg } C. Does anyone know of a glue/cement that can be used safely } upto 1000 deg C! } } Regards } } Alan W Nicholls, PhD } Manager - Electron Microscopy Service } Research Resources Center - East (M/C 337) } Room 100 Science and Engineering South Building } The University of Illinois at Chicago } 845 West Taylor St } Chicago, IL 60607-7058 } } Tel: 312 996 1227 } Fax: 312 996 8091 } Office: Room 110 } } Web site www.rrc.uic.edu }
To all Microscopists, does anyone have a complete good alignment procedure for the Jeol 1200. We would very much appreciate a copy. Thank you very much. Peter Stolzenberg, Pesto Inc. 215-699-6160 Fax 215-699-5275
This is a second chance. I surely need the formula for chromotrope aniline blue and syrius red. It's very important to me at the moment, so I will be able to get some improvement in my transplant kidney bx diagnosis. It's Christmas time, a time for giving, please help me!
Don't need to be stressed I will post to Pathol....
Moisture is often caught within the sample, either in an air pocket or as= it exists in the material. During the curing/heating of the Epoxy, the = moisture vaporizes into a gas, forming a bubble. These bubbles do not es= cape the mount because the Epoxy is too thick at that point.
Thoroughly drying the sample is the only way to ensure this does not happ= en.
I hope this helps.
Sincerely,
Gary Liechty
Peter Steele wrote:
} -----------------------------------------------------------------------= - } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Co= m } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.htm= l } -----------------------------------------------------------------------= =2E } } A recent posting reminded me of a problem that infrequently occurs with= in our lab. In fully processed specimens, the epon blocks will develop b= ubbles as they harden. At times there are so many bubbles that the speci= men will be displaced from an oriented position. To our best efforts, we= have not been able to identify what causes these bubbles. There does no= t seem to be a correlation with the age of any one compound or mold type.= However, making up all new components will often (not always) get rid o= f the bubbles. We use vacuum, both after mixing epon components and agai= n before the epon is cured (i.e., with the tissue embedded in epon). The= epon is EMS Embed-812. Processing is on a Lynx, with glut, cacodylate b= uffers, osmium, graded alcohols, propylene oxide, and uncatalyzed epon. = Typically, after processing, specimens sit under vacuum for 2-4 hours in = epon with catalyst (BDMA) before being placed in the oven. Oven temperat= ure is constant (70C), and curing is overnight. I } have always been curious as to what causes these bubbles and how to avo= id their formation. } } TIA } } P. Steele, Ph.D. } Pathology & Lab. Medicine } All Children's Hospital
-- Gary Liechty Product Application Specialist
Allied High Tech Products, Inc. 2376 E. Pacifica Place Rancho Dominguez, CA 90220
310-635-2466 800-675-1118 US Only 310-762-6808 Fax
www.alliedhightech.com
Products and Equipment for Metallurgical Sample Preparation
Moisture often exists within a sample, in an air pocket or as a component= of the sample/material. During the curing/heating of the Epoxy, the moi= sture vaporizes into a gas, forming a bubble. These bubbles do not escap= e the mount because the Epoxy is too thick at that point.
Thoroughly drying the sample is the only way to reduce this effect.
I hope this helps.
Sincerely,
Gary Liechty
Peter Steele wrote:
} -----------------------------------------------------------------------= - } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Co= m } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.htm= l } -----------------------------------------------------------------------= =2E } } A recent posting reminded me of a problem that infrequently occurs with= in our lab. In fully processed specimens, the epon blocks will develop b= ubbles as they harden. At times there are so many bubbles that the speci= men will be displaced from an oriented position. To our best efforts, we= have not been able to identify what causes these bubbles. There does no= t seem to be a correlation with the age of any one compound or mold type.= However, making up all new components will often (not always) get rid o= f the bubbles. We use vacuum, both after mixing epon components and agai= n before the epon is cured (i.e., with the tissue embedded in epon). The= epon is EMS Embed-812. Processing is on a Lynx, with glut, cacodylate b= uffers, osmium, graded alcohols, propylene oxide, and uncatalyzed epon. = Typically, after processing, specimens sit under vacuum for 2-4 hours in = epon with catalyst (BDMA) before being placed in the oven. Oven temperat= ure is constant (70C), and curing is overnight. I } have always been curious as to what causes these bubbles and how to avo= id their formation. } } TIA } } P. Steele, Ph.D. } Pathology & Lab. Medicine } All Children's Hospital
-- Gary Liechty Product Application Specialist
Allied High Tech Products, Inc. 2376 E. Pacifica Place Rancho Dominguez, CA 90220
310-635-2466 800-675-1118 US Only 310-762-6808 Fax
www.alliedhightech.com
Products and Equipment for Metallurgical Sample Preparation
My collaborators would like to know the blood osmolarity of Panaeus vanamei and of Macrobrachium. They are interested in finding good salines for Panaeus and Macrobrachium that will make their dissected parts happy for physiological experiments, and also a favorite buffer for fixation of these happy parts for electron microscopy. Long ago I used to use Dalton's saline for physilogical experiments on crab parts, but I'm wondering if any of you have other opinions. For fixation I generally use 4% glut in 0.1M sodium cacodylate with 0.35M sucrose for the marine stuff, but I'm willing to entertain other recipes as well.
Thanks in advance for any suggestions!
Mele Kalikimaka, Tina
http://www.pbrc.hawaii.edu/bemf/microangela
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
At the Molecular Imaging Laboratories at the University of California , San Diego, there is an immediate opening for an ultramicrotomist. A candidate should be definitely hands-on familiar with Reichert and Sorvall microtomes as well as with Epon, Spurr, and Araldite embedments. Experience with sectioning of frozen sucrose infused samples is a big plus, but it can be a subject of training on the site. This is an equal employment opportunity. Minorities are strongly encouraged to apply. Starting salary ~$24000.- Candidates are welcome to contact me with any questions.
Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368 phone: 8588223373 fax: 8588223715 pager: 8586161420 e.mail: mmm-at-ucsd.edu e.pager: 1620024619-at-alphapage.airtouch.com www site: http://mil.ucsd.edu/ ftp site: mil.ucsd.edu
Greetings: I recently purchased the Aus-Jena Laboval scope that I Asked your opinion of last month.I am overjoyed with it.It is nearly new and looks and works as if it has been hardly used.I have a few questions some of you may be able to help me with ? The unit is approx.20 years old: 1.) Does anyone have any idea where I can find a factory manual on use & maintenance in English? or a reprint ?
2.) The unit has an accessory polarizer unit between the trinocular eyepiece and the turrent, it is marked 1.25X. I presume this means it increases effective magnification by 1.25.Is this correct ? The Trinocular head has a camera mount and its marked 1X at the camera fitting, and 1.6X at the eyepiece binocular unit.Would I multiply the objective power by both 1.25 and 1.6 to figure the total working magnification ?
3.) Any Idea where I can get an English manual on the polarizer.I have a wooden fitted box full of many different lens plates & sliders etc. but no instructions or anything.Soon I will figure it out however,I would really like to have a manual for this accessory.
4.) I also have a separate darkfield condenser unit,any idea on manuals or instructions ?
Thank you all for taking the time to read this,and special thanks to all who can help point me in the proper direction. Brian Gortney
gortn-at-earthlink.net
PS: I purchased the unit from Mcbain Instruments in Chatsworth Ca. They are a Leica dealer and service facility.I am well pleased with them. I would not hesitate to recommend them. Gerry Burke has been most helpful and knowledgeable.(I have no affiliation with Mcbain)
I find that I can dramatically reduce the chance of bubbles if I pre-dry the empty capsules and their labels in the oven all day or overnight before adding the samples and resin. I got onto this many years ago when I realized I was getting bubbles off the paper labels as well, so I decided they held moisture and/or gas. Works for me!
Good luck, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I have a materials background, so I'm sure I do not understand the process of embedding biological samples. That said, the bubbles could be a result of outgassing your specimen directly in the epoxy. Also, outgassing specimens and epoxy for 2 - 4 hours sounds excessive and could drive off much of the typically aromatic catalyst used for free-radical addition polymerization of epoxies. I typically do not outgas my epoxy (Spurrs), I try to mix it very gently so as not to mix air into it.
On occasion, for very porous problem materials, I will put the epoxy and specimen under vacuum for at most 5 minutes. However, I do not place the specimen in the epoxy during this time, as the surface tension of liquids will impede the evacuation process. I place the specimen on a piece of foil attached to the lip of a small beaker of epoxy. After outgassing in a small bell jar for a few minutes, I gently tap the base plate of the bell jar to knock the specimen into the epoxy, and then leak the chamber back up to atmospheric pressure. In this process you more efficiently evacuate the interstitial spaces of the samples and use atmospheric pressure at 14 psi to force epoxy into the evacuated voids. This has worked well for fine sub-micron sized powder, fabric, sponge, paper, and catalysts.
When embedding pre-processed wet tissue, it is important to first outgas the sample in the lowest molecular weight liquid. Infiltration through serial dilutions of solvent to viscous epoxy then occurs primarily by diffusion, minimizing tissue trauma and damage.
The vacuum pulled should not reach pressures so low that active boiling of the epoxy occurs - a gentle release of small bubbles is all the is needed, and then only to indicate outgassing. There's no reason to outgas dissolved gases - leave them dissolved, it's only necessary to evacuate the voids and trapped gas in your sample prior to embedding.
Cheers,
Phil Swab Engineering Development Deposition Sciences Inc. Santa Rosa, CA 707-566-3718 phil.swab-at-depsci.com
-----Original Message----- } From: Peter Steele [SMTP:STEELEP-at-allkids.org] Sent: Tuesday, December 14, 1999 11:27 AM To: Microscopy-at-Sparc5.Microscopy.Com
A recent posting reminded me of a problem that infrequently occurs within our lab. In fully processed specimens, the epon blocks will develop bubbles as they harden. At times there are so many bubbles that the specimen will be displaced from an oriented position. To our best efforts, we have not been able to identify what causes these bubbles. There does not seem to be a correlation with the age of any one compound or mold type. However, making up all new components will often (not always) get rid of the bubbles. We use vacuum, both after mixing epon components and again before the epon is cured (i.e., with the tissue embedded in epon). The epon is EMS Embed-812. Processing is on a Lynx, with glut, cacodylate buffers, osmium, graded alcohols, propylene oxide, and uncatalyzed epon. Typically, after processing, specimens sit under vacuum for 2-4 hours in epon with catalyst (BDMA) before being placed in the oven. Oven temperature is constant (70C), and curing is overnight. I have always been curious as to what causes these bubbles and how to avoid their formation.
TIA
P. Steele, Ph.D. Pathology & Lab. Medicine All Children's Hospital
} I agree with you that such a side-by-side comparison would be a good } idea.
Yes, this is a very good idea.
Another interesting variable is the paper on which the images are printed. At the Microscopy Society of Southern Africa's recent meeting there was a very informative display of images printed (mainly by different HP and Epson inkjets, if I remember correctly) on a wide variety of papers. This also included SEM images of the paper. I don't remember which turned out to be the "best" paper (or "best value for money" paper) but there certainly was a wide difference between the quality of the images and the fine structure of the papers that were examined. Something that was immediately obvious to me was the wide variety of contrast produced by different papers.
Warm regards from us all down here (in the high 30's C this week)!
Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa R.Cross-at-ru.ac.za tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/emu/index.htm
** remember that ICEM-15 in 2002 is in Durban, South Africa **
Hi I have an AN10000 EDX system. The hard disk has gone down. I usually use NEC D3142 20Mb replacements. Sadly my supplier can no longer source this drive. Does anyone have a suggestion for an alternative drive.
Many thanks
Chris
Chris Gilpin Experimental Officer Biological Sciences EM Unit G452 Stopford Building Oxford Road Manchester M13 9PT phone +44 0161 275 5170 Fax +44 0161 275 5171 http://www.empgu.man.ac.uk
have you considered the possibility that you are simply spinning down any hard debris to the bottom of your capsule which can cause holes in sections and general problems with embedding. This seems to be a classic problem with small pellets especially where you have to spin after each solution and the debris may come from a variety of sources: the sample crystals or deposits in solution (maybe your Cu2SO4 is leaking out of the dialysis tube) bits of broken glass from pipettes etc
The simplest answer is just to trim past this layer, but you could try filtering everything before use (0.2um filters on syringes) and avoiding the use of glassware with these difficult pellets (disposable plastic pasteurs etc). Otherwise encapsulate in agar or try pelleting in haematocrit glass capillaries as others have suggested.
Oh and merry Christmas and happy new Millennium to one and all (the above reply was just an excuse for this greeting really).
Malcolm
Malcolm Haswell Electron Microscopy School of Sciences Fleming Building University of Sunderland SUNDERLAND SR1 3SD Tyne and Wear UK
Tel (0191) 515 2872 e-mail: malcolm.haswell-at-sunderland.ac.uk ----------------------------------------------------- Claudia Hayward-Costa wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Experts, } } I am working with leukemia cells in suspension and spin them } down in conical BEEM capsules prior to fixation. All subsequent } steps (osmication, dehydration, infiltration with epon 812) are } performed in these tubes. } Unfortunately the blocks are very difficult to cut. The tips are with } holes and inside the blocks there are "craters" and spongy areas } that make it very difficult to cut proper sections. } The pellets are minute, therefore I like the convenience of the } conical tubes but I have to cut "big" chunks away with the razor } blade before I get reasonable sections which makes it a quite } wasteful job. } The absolute EtOH is dried over Cu2SO4 in a dialysis tube and the } cells are in filtrated twice within 24 hours. } } I know that one way out of this dilemma would be to handle the } pellet as a tissue - but the experiment is so time consuming that I } still do not want to risk having a crumbling pellet after 2 days work. } } Thanks in advance for your kind help. } } Claudia } } Dr. C. Hayward-Costa } School of Life Sciences } Kingston University } Penrhyn Road, Kingston upon Thames } Surrey KT1 2EE, UK } ++44(0)181 547 2000 x 2240 } Email: c.hayward-at-kingston.ac.uk
Hi Jaclyn, Richard-Allan has purchased Microm. Richard-Allan/Microm will be servicing the equipment in the near future, but for service now please contact Zeiss Service.
Dawn M. Truscott, HT(ASCP) RAS/Microm Instrumentation Team
I am trying to help a student who has been bought a 10-40x stereo zoom microscope with some image recording/analysis equipment. The microscope is totally unbranded - nothing on the body or the eyepieces (except for magnification).
The problem is an apparent mismatch of the lamp intensity in the eyepieces. In the right ocular, the illumination is brighter centrally, and it zooms up concentrically - that's fine. In the left ocular, the brighter central zone zooms up to go out of the field of view, to the left. A centred specimen zooms up concentrically in both oculars. Any suggestions? Is it a phenomenon of the different height of the bulb, which is directly below the stage, and the specimen? The problem is apparently alleviated by putting a piece of velin tissue (thin paper) under the specimen. But this introduces a patterned background.
Keith Ryan Marine Biological Association Plymouth UK
Although established as a tool in materials science and physics, scanning= =20 probe microscopy (SPM) is at the beginning of its application in biomateria= ls=20 science. On 2 April 1998 the first workshop entitled "Scanning Probe Micros= copy=20 in Biomaterials Science, Dentistry and Medicine" was held in Bristol, UK.= =20 What was planned to be a small workshop evolved to be an international conf= erence with high calibre=20 delegates and speakers from all over the world. Encouraged by the success o= f the meeting and supported by international academics and industrial researchers we are organising a 2= nd conference. =20 Since this first conference in 1998 more researchers have applied atomic fo= rce=20 microscopy and related SPM methods in biomaterials science.=20 Therefore a definitive need for a broad scientific exchange between researc= hers involved in these studies exists. This is the purpose of The 2nd International Conference on Scanning Probe = Microscopy in Biomaterials Science,=20 which will be hosted by the University of Bristol and Veeco Instruments Lim= ited.
Contributions should cover, but are not limited to, the following areas:
Imaging of biomaterials surfaces (polymers, metals ceramics etc.)=20 Interfaces between biomaterials and biological materials (e.g. protein-biom= aterial interfaces)=20 Investigation of local properties of biomaterials (mechanical, chemical etc= .)=20 Structural change of biomaterials=20 Aspects of medicine and dentistry relevant for SPM (e.g. SPM on mineralised= tissues or DNA)=20 Instrumental developments in SPM and combination with other methods in the= =20 investigations of biomaterials
Deadlines and dates
1 September 1999: early registration starts 1 January 2000: registration starts 1 April 2000 deadline for abstract submission 1 June 2000 registration closes =96 late registration (at an increased fee = rate) possible until the date of the conference.
Speakers (confirmed):=20 Saul Tendler, Nottingham, UK Roger Marchant, Cleveland, OH, USA Grayson W. Marshall, San Francisco, USA Buddy D Ratner, Seattle, USA Klaus Jandt, Bristol, UK etc.
Poster and presentations sessions: delegates will be able to present poster= s or=20 give 15 min. oral presentations.
---------------------------------------------------------------------------= ----- Dr. rer. nat. Klaus D. Jandt Senior Lecturer in Dental Materials Science and Biomaterials University of Bristol, Department of Oral and Dental Science Lower Maudlin Street, Bristol, BS1 2LY, UK Phone: ++ 44 (0) 117 9 28 44 18, Fax: ++ 44 (0) 117 9 28 47 80 Internet: K.Jandt-at-bris.ac.uk WWW: http://www.dent.bris.ac.uk/Biomaterials/kdj.htm "We make Biomaterials Science work!"
I have certainly experienced the problems that Phil mentions. In fact I would only pump down a chamber slowly and bleed air back in if excessive bubbling occurred. Most specimens only needed a few minutes of outgassing with Spurr's. Perhaps part of your problem is due to the higher viscosity of Epon which as it warms becomes less viscous and releases trapped bubbles.
Malcolm
Malcolm Haswell Electron Microscopy School of Sciences Fleming Building University of Sunderland SUNDERLAND SR1 3SD Tyne and Wear UK
Tel (0191) 515 2872 e-mail: malcolm.haswell-at-sunderland.ac.uk
Phil Swab wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Peter et al, } } I have a materials background, so I'm sure I do not understand the process } of embedding biological samples. That said, the bubbles could be a result } of outgassing your specimen directly in the epoxy. Also, outgassing } specimens and epoxy for 2 - 4 hours sounds excessive and could drive off } much of the typically aromatic catalyst used for free-radical addition } polymerization of epoxies. I typically do not outgas my epoxy (Spurrs), I } try to mix it very gently so as not to mix air into it. } } On occasion, for very porous problem materials, I will put the epoxy and } specimen under vacuum for at most 5 minutes. However, I do not place the } specimen in the epoxy during this time, as the surface tension of liquids } will impede the evacuation process. I place the specimen on a piece of } foil attached to the lip of a small beaker of epoxy. After outgassing in a } small bell jar for a few minutes, I gently tap the base plate of the bell } jar to knock the specimen into the epoxy, and then leak the chamber back up } to atmospheric pressure. In this process you more efficiently evacuate the } interstitial spaces of the samples and use atmospheric pressure at 14 psi } to force epoxy into the evacuated voids. This has worked well for fine } sub-micron sized powder, fabric, sponge, paper, and catalysts. } } When embedding pre-processed wet tissue, it is important to first outgas } the sample in the lowest molecular weight liquid. Infiltration through } serial dilutions of solvent to viscous epoxy then occurs primarily by } diffusion, minimizing tissue trauma and damage. } } The vacuum pulled should not reach pressures so low that active boiling of } the epoxy occurs - a gentle release of small bubbles is all the is needed, } and then only to indicate outgassing. There's no reason to outgas } dissolved gases - leave them dissolved, it's only necessary to evacuate the } voids and trapped gas in your sample prior to embedding. } } Cheers, } } Phil Swab } Engineering Development } Deposition Sciences Inc. } Santa Rosa, CA } 707-566-3718 } phil.swab-at-depsci.com } } -----Original Message----- } } From: Peter Steele [SMTP:STEELEP-at-allkids.org] } Sent: Tuesday, December 14, 1999 11:27 AM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Epon bubbles } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } A recent posting reminded me of a problem that infrequently occurs within } our lab. In fully processed specimens, the epon blocks will develop } bubbles as they harden. At times there are so many bubbles that the } specimen will be displaced from an oriented position. To our best efforts, } we have not been able to identify what causes these bubbles. There does } not seem to be a correlation with the age of any one compound or mold type. } However, making up all new components will often (not always) get rid of } the bubbles. We use vacuum, both after mixing epon components and again } before the epon is cured (i.e., with the tissue embedded in epon). The } epon is EMS Embed-812. Processing is on a Lynx, with glut, cacodylate } buffers, osmium, graded alcohols, propylene oxide, and uncatalyzed epon. } Typically, after processing, specimens sit under vacuum for 2-4 hours in } epon with catalyst (BDMA) before being placed in the oven. Oven } temperature is constant (70C), and curing is overnight. I have always been } curious as to what causes these bubbles and how to avoid their formation. } } TIA } } P. Steele, Ph.D. } Pathology & Lab. Medicine } All Children's Hospital
My niece's science project for fourth grade is liver and related diseases. Does anyone have micrographs of normal or diseased liver that could be shared with her class? Please contact me off-list Thank you very much pamela.neill-at-alconlabs.com
Peter, I also use EMbed-812, but polymerize overnight at 60 degrees. I do not use any vacuum steps and I use DMP-30 instead of BDMA. I don't perform any extra preparation steps to remove moisture from anything (and we're in the SE where it's quite humid in the summer). I have never had a problem with bubbles. I think that 70 degrees is excessive for overnight polymerization. This may be the source of your problem. Call Stacy Kirsch at Electron Microscopy Sciences to troubleshoot this problem. If she or the chemistry people who make EMbed-812 can't suggest a solution, I would be surprised! She's probably trying to unclench her jaw right now, having seen your question!!!
Bob Santoianni Emory University Hospital Atlanta, GA
A colleague has asked for a recommendation for a camera to be used as described below. I would appreciate any recommendations you can make. Since this has been a topic before, perhaps it would be best to reply directly to me.
Thanks so much for any help you can provide.
Damian Neuberger, Ph D Research Scientist Baxter Healthcare, Inc. WG3-2S P.O. Box 490 Round Lake, IL 60073 Tel: 847.270.5888 FAX: 847.270.5897
Microscope: Nikon Eclipse E-400 FITC epifluorescence with 10X and 60X high-dry objectives.
In the midst of lab relocations we have decided to part with some equipment that is not under heavy use.
Available is a Philips 400T STEM with EDAX capabilities which has functioned well for us and an RMC cryo-ultramicrotome MT-6000 XL. The microtome is in excellent condition (like new) with very little use over the years.
We have additional accessories and some parts available as well.
Please respond directly by e-mail or phone.
Prices are negotiable but must be reasonable.
Regards,
Wayne England
============================================ Wayne England Manager, Physical Characterization Bodycote ORTECH Inc. 2395 Speakman Drive, Mississauga, ON, L5K1B3 wengland-at-ortech.on.ca WEB: www.bodycote.com 905-822-4111 Ext.555 FAX:905-823-1446 ============================================
Nestor did this several years ago. He sent around a test image that was fairly representative of the types of images found in microscopy labs. Since he didn't jump into this thread, he probably doesn't want the hassle of setting it up the comparison table again. What I suggest is that someone that is interested in doing it and who is going to attend the M&M MM meeting volunteer to take responsibility to do this. I'm sure that Nestor would send the test image to that person for distribution to those who want to participate. If you are the person who would like the responsibility, contact Stuart McKernan (the M&M MM Program Chair) and ask to do it. I suggest that you coordinate it with Nestor and John Mansfield. Stuart's Email address is mckernan-at-cems.umn.edu
If nobody want to do it, then let's drop the thread.
Just my two cents.
-Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
I have replaced these drives with SSCSI HD drives from old MacIntosh computers they work fine, and only have to be reformatted. Your hardware may also permit } than 20Mb drives, different chip sets in the AN1000 may allow you to use 40 / 80 Mb drives. You'll find out when you format them.
Also check your 5 V power supply. I've had alot of problems with the HD and it turns out that the problem was sometimes the voltage levels. Just unplug the floppy drive and stick a DVM in the power plug. You should get } 5 V. if it drops below 5 then HD action will act as if the drive is dead.
A key thing to check is the connectors from the PS to the Bus. They are silver coated and tarnish. Get a bit of metal polish and clean them off. On my system this made a 0.25 V difference!
Nestor Your Friendly Neighborhood SysOp.
} Hi } I have an AN10000 EDX system. The hard disk has gone down. I usually use NEC } D3142 20Mb replacements. Sadly my supplier can no longer source this drive. } Does anyone have a suggestion for an alternative drive. } } Many thanks } } Chris } } } Chris Gilpin } Experimental Officer } Biological Sciences EM Unit } G452 Stopford Building } Oxford Road } Manchester } M13 9PT } phone +44 0161 275 5170 } Fax +44 0161 275 5171 } http://www.empgu.man.ac.uk
I am trying to find someone to survey and install a ESEM + EDS in the Dallas Area by the end of the year. We are going to evaluate the system, so we are trying to minimize this installation cost. We will probably move it again in about year. Any suggestions? Jeff Day/JD wa5ekh-at-juno.com
Question: Please tell me the minimum magnification needed to recognise spirocheates without doubts. I think of using darkfield and I have to decide if I need to buy a standard condenser plus a light stop or a specialized darkfield condenser. I know that a light stop works only at low magnification.
Dear all } } This is a second chance. I surely need the formula for chromotrope } aniline blue and syrius red. It's very important to me at the moment, } so I will be able to } get some improvement in my transplant kidney bx diagnosis. It's } Christmas time, a time for giving, please help me! } } Don't need to be stressed I will post to Pathol.... } } Thanks } Lucia Caldas } Rua Pres. Backer 234/604 BII } Icarai Niteroi RJ } Brasil 24220-041 ***********************************
This isn't much help,but years ago (in a previous employment) we used picro-sirius red to stain connective tissue elelmnts in heart muscle. I do not have the protocols, and sadly, the investigator who headed that lab has died. A colleague of mine was using it to look at fibroblast cultures (also from heart) about 4 or 5 years ago. The principle investigator in the lab is named Jeffrey Borer. You may find something in his publications. I've check the histo techniques books I have on hand to no avail. You may have more luck posting this question on the HistoNet listserver (HistoNet-at-Pathology.swmed.edu). Send an email with the word "subscribe" in the subject line. That sight is frequented by histologists/pathologists who would have a broader knowlege and resources for these fairly obscure techniques.
Good luck, Lee
Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy Core Facility Manager, Confocal Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
We still have the following items that are now surplus to our requirements in our lab. Any one interested. Buyer collects or pays shipping.=20 All items are as is. You can have them shipped to you for=20 inspection, but you pay the shipping both ways if you don't want the=20 item.
1. A Gatan model 622 image intensified TV camera designed to mount on a JEOL 4000EX (i.e. we have the mounting flange for that instrument and the camera is shielded for use on that instrument. This camera is about 11 years old. $6,000 or make an offer.
2. Two Tracor TN5500 XEDS systems. a. One system has a 30Meg hard disk drive, two 5.25 Syquest removable hard disks (both failed) and two floppy disks one 5.25" and one 8". There are actually two 5.25" disks and two 8" disks in a separate subsystem, but the hard ware only supports two floppies at one time and so we have one of each set up. A standard Tracor keyboard with keypad and monitor is supplied. The system does not have a printer. We modified it so it would run without a printer and if we need print out we have a couple of switch boxes that directs the print out to a Mac (PC can be substituted). We also have the HP=20 plot software and this is directed to a program on the Mac that can=20 then send the plot to a laser printer or can save it for pasting into=20 word processing documents. The system has the imaging package that will allow the computer to control the microscope (it is setup for a JEOL 2000FX) and record STEM and SEM images and XEDS maps. The software includes SMTF and SQMTF. The system has an almost new refurbished light element detector (detects down to C). System also has a license for RT-11, the DEC operating system and it can run an FTP server for removal of spectra and images to a remote computer. $12,000 or make an offer.
b. The second system is floppy based and also has imaging which is setup for an SEM whose manufacturer evades my memory, but if anyone is interested I will obviously find out for you. This system has a Be window XEDS detector with it. $6,000 or make an offer.
3. A Gatan double-tilt Be cup analytical stage for the JEOL 2000FX, Model 646. $3,000 or make an offer.
4. Liquid nitrogen cold stage for JEOL 2000 FX Gatan double tilt (old model 613 upgraded to double tilt). Sample airlock pumps dewar jacket. $6,000 or make an offer.
OK? Let me know if you want more info.
Please note new FAX number.
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 (Leaving a phone message at 936-3352 is preferable to 358-7555) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
Although established as a tool in materials science and physics, scanning= =20 probe microscopy (SPM) is at the beginning of its application in biomateria= ls=20 science. On 2 April 1998 the first workshop entitled "Scanning Probe Micros= copy=20 in Biomaterials Science, Dentistry and Medicine" was held in Bristol, UK.= =20 What was planned to be a small workshop evolved to be an international conf= erence with high calibre=20 delegates and speakers from all over the world. Encouraged by the success o= f the meeting and supported by international academics and industrial researchers we are organising a 2= nd conference. =20 Since this first conference in 1998 more researchers have applied atomic fo= rce=20 microscopy and related SPM methods in biomaterials science.=20 Therefore a definitive need for a broad scientific exchange between researc= hers involved in these studies exists. This is the purpose of The 2nd International Conference on Scanning Probe = Microscopy in Biomaterials Science,=20 which will be hosted by the University of Bristol and Veeco Instruments Lim= ited.
Contributions should cover, but are not limited to, the following areas:
Imaging of biomaterials surfaces (polymers, metals ceramics etc.)=20 Interfaces between biomaterials and biological materials (e.g. protein-biom= aterial interfaces)=20 Investigation of local properties of biomaterials (mechanical, chemical etc= .)=20 Structural change of biomaterials=20 Aspects of medicine and dentistry relevant for SPM (e.g. SPM on mineralised= tissues or DNA)=20 Instrumental developments in SPM and combination with other methods in the= =20 investigations of biomaterials
Deadlines and dates
1 September 1999: early registration starts 1 January 2000: registration starts 1 April 2000 deadline for abstract submission 1 June 2000 registration closes =96 late registration (at an increased fee = rate) possible until the date of the conference.
Speakers (confirmed):=20 Saul Tendler, Nottingham, UK Roger Marchant, Cleveland, OH, USA Grayson W. Marshall, San Francisco, USA Buddy D Ratner, Seattle, USA Klaus Jandt, Bristol, UK etc.
Poster and presentations sessions: delegates will be able to present poster= s or=20 give 15 min. oral presentations.
---------------------------------------------------------------------------= ----- Dr. rer. nat. Klaus D. Jandt Senior Lecturer in Dental Materials Science and Biomaterials University of Bristol, Department of Oral and Dental Science Lower Maudlin Street, Bristol, BS1 2LY, UK Phone: ++ 44 (0) 117 9 28 44 18, Fax: ++ 44 (0) 117 9 28 47 80 Internet: K.Jandt-at-bris.ac.uk WWW: http://www.dent.bris.ac.uk/Biomaterials/kdj.htm "We make Biomaterials Science work!"
I have a thin layer (20 nm) of AlGaN on a substrate. In order to protect this layer during Tripod polishing (cross-section specimen) I have twice had a layer of quartz evaporated onto the AlGaN. I have been very careful in cleaning the surface prior to the deposition. However, in both cases the quartz has not adhered well. I am hoping that perhaps someone could advise me as to the following: 1) Any ideas why the quartz has not adhered well? 2) Is sputtering better than evaporating? 3) Is there an optimal thickness for this protective layer? 4) Is there another material that might work better than the quartz to protect the AlGaN during polishing?
Thank you very much for your consideration of this request.
Sincerely,
Mick Thomas ----------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
If these are the "conical tip" style capsules that I am thinking of (Do they have a long cylindrical tip?) then you may not be getting very efficient exchange down in the tip when you make your fluid changes. If you have water or sometimes ethanol left/trapped in the samples then you can get poor infiltration and soft blocks. When you use these cylindrical tip style Beem capsules then you end up with a very small area at the opening of the cylinder for the exchange to occur. The result is poor infiltration especially with shorter infiltration times. You may be able to get better results by increasing your infiltration time, although given the size of the area over which the exchange has to occur I'd think that you would have to significantly increase the time and perhaps rotate the mixture as well. One of the things that I do is to reduce the depth of the cylindrical section by filling it with 100% resin and polymerizing it before I place the sample in the tip. You can taylor the depth of the cylinder by the amount of resin used to fill the tip. When you are ready to section, just cut the blank resin off the end of the block and section the material. One of the side effects of doing this is to reduce the chatter you get from a long unsupported block tip when you are sectioning. I use these style capsules in this manner when I have small amounts of sample that I cannot afford to loose. The method allows me to make a small well to catch the sample, but not have it out on the end of a long cylinder when I go to cut it. As far as sample prep goes, I have had problems on occasion with infiltration when I have hard pelleted my material . Softer pellets seem to infiltrate better -- more spaces in the material to fill with resin? I have also worked with pelleted material that has stayed together after post fixation with osmium and have treated it as I would a tissue. Not a very comfortable feeling though! All in all, you may have to consider using the agarose method for holding your pellets together. Greg
-- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------.
} } Dear Experts, } } I am working with leukemia cells in suspension and spin them } down in conical BEEM capsules prior to fixation. All subsequent } steps (osmication, dehydration, infiltration with epon 812) are } performed in these tubes. } Unfortunately the blocks are very difficult to cut. The tips are with } holes and inside the blocks there are "craters" and spongy areas } that make it very difficult to cut proper sections. } The pellets are minute, therefore I like the convenience of the } conical tubes but I have to cut "big" chunks away with the razor } blade before I get reasonable sections which makes it a quite } wasteful job. } The absolute EtOH is dried over Cu2SO4 in a dialysis tube and the } cells are in filtrated twice within 24 hours. } } I know that one way out of this dilemma would be to handle the } pellet as a tissue - but the experiment is so time consuming that I } still do not want to risk having a crumbling pellet after 2 days work.
} } Thanks in advance for your kind help. } } Claudia } } Dr. C. Hayward-Costa } School of Life Sciences } Kingston University } Penrhyn Road, Kingston upon Thames } Surrey KT1 2EE, UK } ++44(0)181 547 2000 x 2240 } Email: c.hayward-at-kingston.ac.uk
I would like to add to Gary's note. "Traditionally" these media, prior to curing at 65 or 70 were placed in a lower temperature, say 37 for at least 2 hours. The reason is that at those temperature the media set more slowly, but they are at a lower viscosity for a longer time. During this time bubbles can escape.
Peter Steele's method does not seem to include the lower temperature step. The vacuum infiltration with a high viscosity medium at room temperature may not release all valatiles. Worse still, if the vacuum is a touch too high the medium may boil and lots of bubbles are produced, which may not escape to the surface in Epon at room temperature.
When vacuum infiltrating most people pour the resin over the specimen at ambient pressure. When evacuating, bubbles may escape the specimen but do not grow large enough to rise in the viscous medium. Vacuum infiltration of porous specimen is better with the specimen under vacuum and while under vacuum adding the resin. This can be done with very simple equipment and a little ingenuity. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Wednesday, December 15, 1999 10:52 AM, Gary Liechty [SMTP:garyliechty-at-att.net] wrote: } } } Dear Mr. Steele, } } Moisture often exists within a sample, in an air pocket or as a component of } the sample/material. During the curing/heating of the Epoxy, the moisture } vaporizes into a gas, forming a bubble. These bubbles do not escape the } mount because the Epoxy is too thick at that point. } } Thoroughly drying the sample is the only way to reduce this effect. } } I hope this helps. } } Sincerely, } } Gary Liechty } } } Peter Steele wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } A recent posting reminded me of a problem that infrequently occurs within } } our lab. In fully processed specimens, the epon blocks will develop bubbles } } as they harden. At times there are so many bubbles that the specimen will be } } displaced from an oriented position. To our best efforts, we have not been } } able to identify what causes these bubbles. There does not seem to be a } } correlation with the age of any one compound or mold type. However, making } } up all new components will often (not always) get rid of the bubbles. We use } } vacuum, both after mixing epon components and again before the epon is cured } } (i.e., with the tissue embedded in epon). The epon is EMS Embed-812. } } Processing is on a Lynx, with glut, cacodylate buffers, osmium, graded } } alcohols, propylene oxide, and uncatalyzed epon. Typically, after } } processing, specimens sit under vacuum for 2-4 hours in epon with catalyst } } (BDMA) before being placed in the oven. Oven temperature is constant (70C), } } and curing is overnight. I } } have always been curious as to what causes these bubbles and how to avoid } } their formation. } } } } TIA } } } } P. Steele, Ph.D. } } Pathology & Lab. Medicine } } All Children's Hospital } } -- } Gary Liechty } Product Application Specialist } } Allied High Tech Products, Inc. } 2376 E. Pacifica Place } Rancho Dominguez, CA 90220 } } 310-635-2466 } 800-675-1118 US Only } 310-762-6808 Fax } } www.alliedhightech.com } } Products and Equipment for Metallurgical Sample Preparation } }
I tried for the first time to produce carbon extraction replicas of steel containing several types of precipitates (TiC, TiS, TiN, ...). I produced a disk of 3mm and thinned it electrolytically. On this specimen I evaporated carbon and removed the steel disk with bathing it in Nital. I come up with thin carbon layers with lots of precipitates on, but I am quite sure that their distribution is not representative for their original positions on the surface of the steel specimen. Any tips and tricks how to handle this kind of preparation in detail?
Petra -------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public - Gabriel Lippmann Laboratoire d'Analyse des Materiaux (LAM) 162a, av. de la Faiencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpgl.lu Visit our WWW site! http://www.crpgl.lu/~wahlbrin
Is the problem a specular reflection off the bottom plate? If the illumination beam is giving a specular refletion it could easily be aligned to the one optical train and not to the other. I would think that you wouldn't want it going down either, since what you want to see is the light scattered from the specimen, and not the light bouncing off the glass.
best regards mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
Meeting Announcement and Call for Student Poster Abstracts
18th Annual Meeting of the Florida Society for Microscopy and 28th Annual Symposium of the Florida Chapter of the American Vacuum Society
March 13-16, 2000
The Florida Society for Microscopy will hold its annual meeting March 13 and 14 in Orlando at the University of Central Florida. This occasion marks the third meeting of FSM on the campus of the University of Central Florida. FSM will hold technical meetings on Monday March 13 and Tuesday March 14 with sessions covering the Biological, Physical and Material Sciences. Invited speakers will give presentations of their work and we plan to hold a workshop on Digital Imaging for Monday afternoon. Short courses on a variety of topics will be presented by the AVS on Wednesday and Thursday. A vendor equipment exhibit will be held Monday March 13 and Tuesday March 14. We expect to have approximately 45 microscopy- related vendors at the meeting. Student Poster Session: Abstract deadline: January 10, 2000. A student poster session will be held on Monday March 13. Graduate and Undergraduate students in the Biological, Physical and Material Sciences utilizing microscopy in their research efforts are invited to participate in this session by submitting abstracts of their work and presenting their posters at the meeting. We ask that faculty encourage their students to take part in this educational experience. FSM and AVS offer financial incentives to students participating in the poster session, including partial support for travel (within state of Florida), hotel and meal expenses. All competing students will receive a one year paid student membership in AVS or MSA. Grand prizes for the poster sessions can include an expense paid trip (up to $1500) to the 2000 AVS national meeting or to the 2000 MSA national meeting. AVS and FSM are committed to furthering science education and teaching. Plan to be a part of this exciting meeting by volunteering as a judge. We always need plenty of judges and ask faculty members and industry scientists to spend some time helping to guide future scientists. For more information, faculty advisors can contact Larry Plew at 407-371-6915 or plew-at-lucent.com For more information about the meeting contact Brenda Prenitzer, FSM President at 407-823-3680 or bsp101-at-worldnet.att.net or Jo Ann Moore, FSM Vice President at 813-974-9446 or jamoore-at-com1.med.usf.edu
Dear subscribers, We are gathering information to update our scanning EM facility. I was wondering about the latest in cpd's these days. We have an old Polaron, completely manual control that could be refurbished. I have also used an automatic type cpd (ie Tousimis) that seems easier for the novice/occasional user to operate. What would the experts recommend?-especially those from a general use facility. Thanks in advance.
Dear all, I would like to know how to stain by means of histochemical technique both mitochondria and lisosomal vescicles in cultured dendritic cells (formalin-fixed). Many thanks.
} Claudia, } } You can use the Microfuge tubes for all your EM fixation and embedding.. } I have done this before with sea urchin eggs membranes in suspension.. you just spin them down in eh microfuge tube and can do the entire embedding and polymerization in the tubes... and I have not had a problem encountered before... } } } Eric } UCLA } Dept. Pathology } Electron Microscopy Lab
Why not just heat the epon mixture slightly before adding the accelerator to it to get rid of the bubbles... Out here we mix the Pella Eponate 12, NMA, and DDSA and then heat the mixture in the oven for 5-10 minutes and the bubbles disappear and then let it cool and then add the DMP30, or BDMA accelerator...
Has worked just fine here for the 10 months I have been here in the lab....
Eric UCLA Dept. of Pathology Electron Microscopy Lab
Your description sounded like a very brief, but in all essential details, accurate, recipe for making extraction replicas from steels. The only modification I would have suggested is that you etch the sample in Nital for a short time (depending on the exact composition of the steel and the precipitates you are trying to analyse) before depositing the carbon.
Why do you think your replicas are not representative of the precipitates in the steel?
Tony Garratt-Reed.
} I tried for the first time to produce carbon extraction replicas of steel } containing several types of precipitates (TiC, TiS, TiN, ...). } I produced a disk of 3mm and thinned it electrolytically. On this specimen } I evaporated carbon and removed the steel disk with bathing it in Nital. } I come up with thin carbon layers with lots of precipitates on, but I am } quite sure that their distribution is not representative for their original } positions on the surface of the steel specimen. } Any tips and tricks how to handle this kind of preparation in detail? } } Petra } -------------------------------------------------------------- } Dr. Petra Wahlbring } Centre de Recherche Public - Gabriel Lippmann } Laboratoire d'Analyse des Materiaux (LAM) } 162a, av. de la Faiencerie L-1511 Luxembourg } tel. +352-466644-402 fax +352-466644-400 } e-mail: petra.wahlbring-at-crpgl.lu } Visit our WWW site! http://www.crpgl.lu/~wahlbrin } }
1) Vaccum applied to liquid epoxy is counterproductive. The inside of your jar is gooey! Right? What is that? Your accelerator has the lowest vapor pressure (probably) and is the first to go. Do not use vaccum. It is not necessary.
2) You are exposing tissue to uncatalyzed epoxy. This idea was common in the 60's, but is long outdated. You will always get suboptimal results if you first expose tissue to uncatalyzed resin, and then catalyzed resin. It also appears that you let the tissue "sit" in catalyzed resin. Nothing happens when you "sit tissue". Very little exchange of fluid happens. Diffusion barriers will occur and cause trouble. Your tissue should be in motion on a rotator at all times when infiltration is to be achieved.
3) I have a lot of experience with the LYNX. I found that infiltration is difficult if routine times are used. Infiltration times need to be lengthened considerably in order to achieve good infiltration. (And never use uncatalyzed resin)
Will this eliminate bubbles? I don't know, but I do know that the above is good procedure which should eliminate problems.
Good luck, Hildy Crowley Sr. Electron Microscopist University of Denver Denver, CO
I am research assistant to department of physics to University of KIRIKKALE in TURKEY.I study about "phase transitions of alloys" and" shape memory materials" with JEM3010 ElEctron Microcopy.So I am responsible it. Does anyone know objective focus range for 200KV, 100KV to JEM3010?Besides Which films do you use for Electron Microsocopy? We can change sensitive range (2 between 20) for films. Thaks for your interested.
Erdem YASAR EM Laboratory erdem.yasar-at-physics.org
----------------------------------------------- FREE! The World's Best Email Address -at-email.com Reserve your name now at http://www.email.com
We are doing some study on the formation mechanism of silicide produced by laser processing. We use TEM to identify the interfacial phases through microdiffraction and HR-TEM imaging. To simulate the images obtained, we need to create unit structures. But for some of the silicides such as tetragonal Ti5Si4 and C40 TaSi2, we cannot create the unit structures though we know their space groups due to the lack of base atom positions. Is there any source (handbook, data base, etc.) from which we can get the information, or any other method to create the unit cell structures?
Best wishes,
Kun Li
Kun Li, Ph. D
Mailing address: Institute of Materials Research and Engineering 3 Research Link, Singapore 117602
Office: BLK S13, #02-13d, National University of Singapore Lower Kent Ridge Road, Singapore 119260
It probably is a problem of the difference in height. A proper sub-stage illuminator needs to more than a simple lamp in order to provide an even illumination across the field of view, particularily for a binocular scope. If you have a bare bulb then you have essentially a point light source that is at a considerably larger distance to the objective than the sample. At the factory, they aligned the instrument for the right optic path to be inline with the sample and the lamp, but you can not also adjust the left for the same conditions because of the parallax.
If they can put up with the reduced illumination, one simple solution may be a finely ground glass or translucent diffuser placed between the lamp and sample. You'll have to adjust the height for the best balance between even illumination (higher) with no obvious texturing (lower, less focussed). There are also small commercial light boxes that work well with low mag microscopes, but many of the lower priced scopes may not have enough room for this. If there are no markings on it, then the scope may well be one of the Chinese or Russian low-end scopes that are flooding the market. Not a real problem if it does what you need, but don't expect to be able to find accessories or options that work with that particular model.
Allen R. Sampson Advanced Research Systems 317 North 4th. Street St. Charles, IL 60174 PH 630.513.7093 FAX 630.513.7092 Email: ars-at-sem.com
-----Original Message----- } From: Keith Ryan [SMTP:KPR-at-wpo.nerc.ac.uk] Sent: Wednesday, December 15, 1999 6:55 AM To: STEELEP-at-allkids.org; Microscopy-at-sparc5.microscopy.com
Could anyone please tell me of any website which describes the basics of SEM, suitable for a student (who has included some SEM work done by our department) to help in writing up his thesis?
Thanks in advance,
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
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JEOL has a nice Guide to Scanning Microscope Observation at the following site:
http://www.jeol.com/docs.html
Hope this will help.
Yours sincerely, Jesper
---------------------------------------------------- Jesper Vejloe Carstensen Research Scientist, M.Sc., Ph.D. Materials Research Department Risoe National Laboratory P.O. Box 49 DK-4000 Roskilde, Denmark Phone: +45 4677 5776 Fax: +45 4677 5758 E-mail: jesper.v.carstensen-at-risoe.dk Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm ----------------------------------------------------
-----Original Message----- } From: Robert H. Olley [mailto:r.h.olley-at-reading.ac.uk] Sent: 16. december 1999 10:34 To: Microscopy Newsgroup Cc: #
To clear up freshly mixed Epon, especially older more viscous stocks, we transfer it to a disposable centrifuge tubes and spin it at top speed in a table top centrifuge for 5 to 10 minutes. The vigorously mixed Epon is "milky" before the centrifugation and is clear afterwards. This outdated Epon is often used to make pen holders, vial holders for post staining etc. Anyone out there use old Epon in other ways?
Mike Baxter Lehman College Bronx, NY ___________________________________________________________________ Why pay more to get Web access? Try Juno for FREE -- then it's just $9.95/month if you act NOW! Get your free software today: http://dl.www.juno.com/dynoget/tagj.
As Scott said we did this many years ago, and of course, being the pack rat that I am I still have stored in my files the original prints from various printers (I want to see how well they archive).
In any event, every year at the computer workshop held at the Microscopy & Microanalysis meeting (see http://www.msa.microscopy.com) we allow people to bring in copies of prints made on their printer. and if they leave them with me they all go into the archive.
There is a standard test image which you can download by FTP the image is called.
It is a TIFF image and has been stored in both PC and Mac Formats.
The 300 dpi version is ~ 7 Mb the 100 dpi version is ~ 800 K.
A number of printer manufacturers, who exhibit at the meeting use this as a demo image. It has a range of images from all fields from Physical to Life Sciences (all are intentionally gray scale).
Nestor Your Friendly Neighborhood SysOp.
================================================================== Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." so I bought a G3 Mac
I think that the printer comparison suggested for the Philadelphia meeting is a great idea; I have two comments and a related question. The comments: 1) Nestor & John have too much to do as it is; someone should volunteer to help with this project. 2) The question about printing paper/ink structure suggests an excellent topic for someone who wants to apply for a Professional Technical Staff award to attend the meeting (see the meeting announcement, pg. 13). And the question: I've learned (the hard way) about the fragile, sticky surface of inkjet prints. Has anyone tried the new Gepe Inkjet Fixative yet? Does it work well?
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
} } } "Stein Lava" Question's: } } Any here who uses the Axiovision image program from Zeiss, any pro } or cons? } } Is it a good general image processing program? } } Has any one compared it with the KS series of programs also from } Zeiss? } } Best regards } } Stein Lava } } mailto:Haga2000-at-yahoo.com
I am looking for a eyepiece graticule in order to keep track of what fraction of a slide that I am counting. I am hoping to find a square with a crossmark in the middle. I don't want too many unnecessary lines as it will make my scoring that much more difficult. If anyone knows where I can find something similar to this I would greatly appreciate any help Thanks Jennifer
Jennifer Berger Senior Technical Associate Lovelace Respiratory Research Institute Albuquerque,NM (505)845-1225
Lucia, What is it you are trying to demonstrate with this stain? It sounds like you could try a Masson's Trichrome to get the same results with stains a little more commonly available (at least here in the states). If you are interested, contact me off list and I will fax or mail you a procedure. if you have not yet done so, you could also post this question to the histonet at Histonet-at-pathology.swmedu.edu. they always seem t come up the answer. Wanda ---------- } From: Maria Lucia Ribeiro Caldas To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Dear all
This is a second chance. I surely need the formula for chromotrope aniline blue and syrius red. It's very important to me at the moment, so I will be able to get some improvement in my transplant kidney bx diagnosis. It's Christmas time, a time for giving, please help me!
Don't need to be stressed I will post to Pathol....
I am sure they have what you are looking for. Your description sounds like a Whipple disc, but they have many variations of that. Likewise, they can "custom make" anything you want.
***I have no corporate or personal affiliation nor financial interests with KRI, Inc.***
Good Luck,
Lawrence Kordon Nikon, Inc. nikon-at-jagunet.com (800) 626-4566 x3053
"Berger, Jennifer" wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I am looking for a eyepiece graticule in order to keep track of what } fraction of a slide that I am counting. I am hoping to find a square with a } crossmark in the middle. I don't want too many unnecessary lines as it will } make my scoring that much more difficult. If anyone knows where I can find } something similar to this I would greatly appreciate any help } Thanks } Jennifer } } Jennifer Berger } Senior Technical Associate } Lovelace Respiratory Research Institute } Albuquerque,NM } (505)845-1225
What about to create website with sample from different printers on different paper.,
I know that on Canon bubble-jet I get fantastic pictures on some type High resolution paper and something horrible on ordinary paper...
Keep care and be of good cheer.
Regards
(name) Vratislav Richard Eugene Maria John Baptist (surname) of Bejsak (Bayshark)-Collorado-Mansfeld
website: http://www.coleoptera.org listserver: coleoptera on www.egroup.com Coleoptera - Australia, Tenebrionidae of World (incl. Lagriinae, Alleculinae)
University of Sydney The Wentworth Bldg., Box 62 NSW 2006 AUSTRALIA phone : +61 414 540 465 email: vratislav-at-bigfoot.com ricardo-at-ans.com.au (before Ricardo-at-compuserve.com and ricardo-at-login.cz )
Only after the last tree has been cut down, only after the last river has been poisoned, only after the last fish has been caught, only then will you find that money can not be eaten.' CREE INDIAN PROPHECY.
Incoming mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com).
Jennifer, Try Klarman Rulings. (800) 252-2401 They will need to know the diamter to fit into your eyepiece.
Regards, Glen
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
On Thu, 16 Dec 1999, Berger, Jennifer wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am looking for a eyepiece graticule in order to keep track of what } fraction of a slide that I am counting. I am hoping to find a square with a } crossmark in the middle. I don't want too many unnecessary lines as it will } make my scoring that much more difficult. If anyone knows where I can find } something similar to this I would greatly appreciate any help } Thanks } Jennifer } } Jennifer Berger } Senior Technical Associate } Lovelace Respiratory Research Institute } Albuquerque,NM } (505)845-1225 } }
You don't mention what the substrate material is. If it is sapphire, you should consider the small angle cleavage technique if you do not need a site specific sample. Several people (including myself) have gotten very nice samples using it. When I visited Univ. of Ill, we made four good samples in about 2 hours from GaN on sapphire. There are a number of benefits of the technique. -Scott
Scott D. Walck, Ph.D. PPG Industries, Inc. Glass Technology Center Guys Run Rd. (packages) P. O. Box 11472 (letters) Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office) (412) 820-8161 (fax)
"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies." --
} -----Original Message----- } From: Mick Thomas [mailto:mgt3-at-ccmr.cornell.edu] } Sent: Wednesday, December 15, 1999 1:06 PM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Quartz deposition } } } -------------------------------------------------------------- } ---------- } The Microscopy ListServer -- Sponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html } } } -------------------------------------------------------------- } ---------. } } } Fellow microscopists, } } I have a thin layer (20 nm) of AlGaN on a substrate. In } order to protect } this layer during Tripod polishing (cross-section specimen) I } have twice } had a layer of quartz evaporated onto the AlGaN. I have been } very careful } in cleaning the surface prior to the deposition. However, in } both cases } the quartz has not adhered well. I am hoping that perhaps } someone could } advise me as to the following: } 1) Any ideas why the quartz has not adhered well? } 2) Is sputtering better than evaporating? } 3) Is there an optimal thickness for this protective layer? } 4) Is there another material that might work better than the } quartz to } protect the AlGaN during polishing? } } Thank you very much for your consideration of this request. } } Sincerely, } } Mick Thomas } ----------------------------- } Mick Thomas } UHV-STEM Laboratory } E-1 Clark Hall } Cornell University } Ithaca, NY 14853 } } Phone: 607-255-0650 } Fax: 607-255-7658 } e-mail: mgt3-at-msc.cornell.edu }
Jennifer: Try Edmund Scientific for an inexpensive solution at www.edmundscientific.com I have no affiliation with them however,I have been satisfied with their products.They have an assortment of these and are quite helpfull in this area. Good luck Brian Gortney gortn-at-earthlink.net
I'm now starting work on charging of thin films under electron beam irradiation. The film thickness is 5 to 40 nm. Electron energy is 100 to 500 kV. Any material is of interest - insulators, metals, ceramics, semiconductors etc. I need help on finding literature and papers on the subject - theory, models, experiments etc. Any info will be appreciated.
Best regards,
Rado
--------------------------------------------------------------------- Radostin Danev Laboratory of Ultrastructure Research National Institute for Physiological Sciences Myodaiji-cho, Okazaki 444-8585, JAPAN e-mail: rado-at-nips.ac.jp ---------------------------------------------------------------------
2nd International Conference on Scanning Probe Microscopy in Biomaterials Science
23 June 2000
Holiday Inn Crowne Plaza Hotel Bristol, England
Official website: http://www.dent.bris.ac.uk/biomaterials/spm2000/
Although established as a tool in materials science and physics, scannin= g probe microscopy (SPM) is at the beginning of its application in biomaterials science. On 2 April 1998 the first workshop entitled "Scanning Probe Microscopy in Biomaterials Science, Dentistry and Medicine" was held in Bristol, UK. What was planned to be a small workshop evolved to be an international conference with high calibre delegates and speakers from all over the world. Encouraged by the success= of the meeting and supported by international academics and industrial researchers we are organising a 2nd conference.
Since this first conference in 1998 more researchers have applied atomic force microscopy and related SPM methods in biomaterials science. Therefore a definitive need for a broad scientific exchange between researchers involved in these studies exists. This is the purpose of The 2nd International Conference on Scanning Prob= e Microscopy in Biomaterials Science, which will be hosted by the University of Bristol and Veeco Instruments Limited.
Contributions should cover, but are not limited to, the following areas:
Imaging of biomaterials surfaces (polymers, metals ceramics etc.) Interfaces between biomaterials and biological materials (e.g. protein-biomaterial interfaces) Investigation of local properties of biomaterials (mechanical, chemical etc.) Structural change of biomaterials Aspects of medicine and dentistry relevant for SPM (e.g. SPM on mineralis= ed tissues or DNA) Structural biology or biophysical aspects Instrumental developments in SPM and combination with other methods in th= e investigations of biomaterials
Deadlines and dates
1 September 1999: early registration starts 1 January 2000: registration starts 1 April 2000 deadline for abstract submission 1 June 2000 registration closes =96 late registration (at an increased fe= e rate) possible until the date of the conference.
Speakers (confirmed): Saul Tendler, Nottingham, UK Roger Marchant, Cleveland, OH, USA Grayson W. Marshall, San Francisco, USA Buddy D Ratner, Seattle, USA Klaus Jandt, Bristol, UK etc.
Poster and presentations sessions: delegates will be able to present post= ers or give 15 min. oral presentations.
----------------------------------------------------------------- Dr. rer. nat. Klaus D. Jandt Senior Lecturer in Dental Materials Science and Biomaterials University of Bristol, Department of Oral and Dental Science Lower Maudlin Street, Bristol, BS1 2LY, UK Phone: +44-117-9284418, Fax: ++44-117-9284780 Internet: K.Jandt-at-bris.ac.uk WWW: http://www.dent.bris.ac.uk/Biomaterials/kdj.htm "We make Biomaterials Science work!"
At 10:39 17/12/99 +0900, Radostin Danev wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
Dear, Dr. Rado
Many techniques and experimental methods have been developed, in charging investigation on dielectric under electron irradiation. But in most cases= for primary beam energy ranging from some hundreds eV to 30 keV, but a think= that the principles are the same.=20
Experiments
Numerous experimental techniques have been proposed on charging of = insulators under electron irradiation and the surface potential may be deduced .=20
- In AES, Vs is obtained from the peak energy shift of the Auger lines or = of the secondary electron [1,2].
- In EPMA it is obtained from the high energy cut-off (Duane Hunt' limit) of the X-ray bremsstrahlung emitted from the sample [3].=20
- The mirror Method [4,5,6] . This technique consists first to implant a charge in the sample under high electron beam energies and then to scan the electron irradiated area at low ones. The negative implanted charge which plays the role of an electrostatic mirror reflects the primary incidents electrons in the vacuum. The resulting microscope chamber image is then used to deduce quantitative information on the amount of trapped charge.=20
- Recent works [7,8] have proposed to follow the trapped charge during the electron injection by recording the absorbed current, or by lying the= dynamic image distortion to the electric field generated in the vacuum [9].
Basses, Modelling and theory=20
- Bases : There is some good papers dealing with a bases of the= charging effect : here I give you a list ones of them :
* Cazaux, J. Appl. Phys. 85, 1137 (1999) ( Very good for the understanding and the references there in ). * D. C. Joy, Scanning 11, 1 (1989). * D. C Joy and C. S. Joy , Micron. 27, 247 (1996).
I have more references, but I don=92t know, What are you interesting about ?= ( Trapping, Dielectric characterisation using electron beam =85=85 ? ) If you need some other references or information=92s , please contact my =85= =85=85=20
Best Regards,
Mohamed Belhaj.
References:
[1] A. Melchinger and S. Hofmann, J. Appl. Phy. 78, 6224. [2] H. Guo, W. Maus-Friedrichs and V. Kempter, Surf. Interf Anal. 25, 390 (1997). 3] G. F. Bastin and H. J. M. Heijligers, in Electron Probe Quantification, Edited by K. F. J. Heinrich and D. E. Newbury ( Plenum, New York, 1991), p. 193
[4] J. P. Vigouroux, J. P. Duraud, A. Le moel and C. Le Gressus and D.L. Griscom, J. Appl. Phys. [, 5139 (1985). [5] C Le Gressus, F. Valin, H. Henriot, M. Gautier, J P. Duraud, T. S. Sudarshan, R. G. Bommakanti and D. R Tallent, J. Appl. Phys. 69, 6325= (1991). [6] B. Vallayer, G. Blaise and D. Treheux, Rev Scient Inst, 70, 3102 (1999). [7] J. Bigarr=E9, S. Fayeule, O. Paulhe, D. Treheux, IEEE Annual Report, 101 (1997). [8] A. Berroug, J. Bigarr=E9, S. Fayeule, D. Treheux IEEE Annual Report, 97 (1997). [9] M. Belhaj, S. Odof, K. Msellak, and O. Jbara : To appears in J. Appl. Phys.
I give you the address of professor Kotera in Japan ( he was working on charging effect ) Department of Electronic Engineering, Osaka Institute of Thechnology, Omiya, Asahi-ku, Osaka, Japan =20
An entomologist colleague of mine requires a good quality light microscope = to take with her out to do field work. It has to be simple to use, light = weight, "rugged" and has to withstand travelling as cargo to many = destinations near and far. Her lab microscopes all are equipped with = fibre optic illumination, which, of course, would not be appropriate for = field use, so she would also like to know what her options might be for = illumination.
Thanks in advance for any help you can provide. Please contact me offline = and I will forward the messages to her.
Happy Holidays to all!
Paula.
Paula Allan-Wojtas Research Scientist, Food Microstructure Agriculture and Agri-Food Canada Atlantic Food and Horticulture Research Centre Kentville, Nova Scotia, Canada B4N 1J5
Dear JoAnn, I have a Tousimis here and it works like a gem. With all the variables to contend with in EM I find it a help.
Sincerely, Maria
Maria Fazio-Zanakis Bioimaging and Molecular Histology Aventis Pharmaceuticals 1-908-231-3357 Fax: 1-908-231-3962 Email: Maria.Fazio-Zanakis-at-Aventis.com
-----Original Message----- } From: JoAnn Buchanan [mailto:redhair-at-leland.Stanford.EDU] Sent: Wednesday, December 15, 1999 1:54 PM To: microscopy-at-sparc5.microscopy.com
Dear subscribers, We are gathering information to update our scanning EM facility. I was wondering about the latest in cpd's these days. We have an old Polaron, completely manual control that could be refurbished. I have also used an automatic type cpd (ie Tousimis) that seems easier for the novice/occasional user to operate. What would the experts recommend?-especially those from a general use facility. Thanks in advance.
Try http://distans.livstek.lth.se:1080/foodmi.htm and look for foods under = microscope.
Ann Fook Yang EM Unit, Eastern Cereal and Oilseed Research Centre, Rm 2091, K.W. Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
Phone: 613-759-1638 Fax; 613-759-1701
} } } "Robert H. Olley" {r.h.olley-at-reading.ac.uk} 12/16 4:33 AM } } } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html=20=
Could anyone please tell me of any website which describes the basics of SEM, suitable for a student (who has included some SEM work done by our department) to help in writing up his thesis?=20
Try Applied Image: 716-482-0300 (Rochester, NY). They make a variety of reticles and graticules for microscopy.
Good hunting! Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education}
At 01:36 PM 12/16/99 -0700, Berger, Jennifer wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Nestor makes a good point re: your voltage....I have two aging An10000 systems and find that even 4.85V is not enough. A few other things to try: 1) the connector ribbon from the drive controller board to the drive will get corroded on both ends. You may hear the HD spinning but the system won't "see" it. 2) As a last-ditch effort, stick your HD in the freezer for about half an hour, do your best to wipe off condensation, and plug it back in. I have an original 20 MB drive that has been revived in this way at least a half dozen times. The lubrication gets "gummy" over time; freezing will free the mechanism and generally once you can get the disk spinning it will run until the next power failure (or someone turns off the computer!) On my original drives, there is also an armature which you can turn by hand to loosen it up...don't worry, it finds "home" position on powerup. The things we will try in desperation....
Matthew J. Lynn, Ph.D. Center for Advanced Microscopy University of Miami (305)284-4736 mlynn-at-miami.edu
On Wednesday, December 15, 1999 9:15 AM, Nestor J. Zaluzec [SMTP:zaluzec-at-sparc5.microscopy.com] wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Chris } } I have replaced these drives with SSCSI HD drives from old MacIntosh } computers } they work fine, and only have to be reformatted. Your hardware may also } permit } than 20Mb drives, different chip sets in the AN1000 may allow you to } use 40 / 80 Mb drives. You'll find out when you format them. } } Also check your 5 V power supply. I've had alot of problems with the HD and } it turns out that the problem was sometimes the voltage levels. Just unplug } the floppy drive and stick a DVM in the power plug. You should get } 5 V. } if it drops below 5 then HD action will act as if the drive is dead. } } A key thing to check is the connectors from the PS to the Bus. They are } silver coated and tarnish. Get a bit of metal polish and clean them off. On } my system this made a 0.25 V difference! } } Nestor } Your Friendly Neighborhood SysOp. } } } } } Hi } } I have an AN10000 EDX system. The hard disk has gone down. I usually use NEC } } D3142 20Mb replacements. Sadly my supplier can no longer source this drive. } } Does anyone have a suggestion for an alternative drive. } } } } Many thanks } } } } Chris } } } } } } Chris Gilpin } } Experimental Officer } } Biological Sciences EM Unit } } G452 Stopford Building } } Oxford Road } } Manchester } } M13 9PT } } phone +44 0161 275 5170 } } Fax +44 0161 275 5171 } } http://www.empgu.man.ac.uk } }
Brian Gortney says: } Jennifer: } Try Edmund Scientific for an inexpensive solution at } www.edmundscientific.com
I seems that Edmund's has only *reticles*, but not *graticules*? I.e. measuring things but not counting ones?
I'd be happy to be proven wrong here - I need a graticule myself (21mm disk, 5mm of squares 10x10). -- Regards, Uri uri-at-watson.ibm.com -=-=-=-=-=-=- {Disclaimer}
I need schematic diagrams for circuit boards in a Balzers SCD 040 sputter coater. Does anyone have the factory service manual, or an address to get me started?
Thank You, Thank You, Thank You. :0)
Paul
Paul Grover Chief Microscopist and Bottle Washer Microvista Laboratory Lafayette, IN
Applications of Scanning Microscopy in Forensic Science
The "Applications of Scanning Microscopy in Forensic Science" symposium (part of SCANNING 2000 - please see below) has been very well attended since it's initiation in 1993. Due to continual growth over the last seven years and the overall success of the forensics symposium, an additional day of forensic papers has been added to the symposium. Combined with the popular one day "Scanning Microscopy in Forensic Science" short course, the forensic scientist/student will be able to attend three consecutive (and full) days of instruction and current research papers all devoted to scanning microscopy applications in forensic science.
You are encouraged to submit an abstract for platform or poster consideration and be a part of the new millennium Forensics Symposium. Outstanding papers will be considered for an invitation to publish in SCANNING, The Journal of Scanning Microscopies.
In addition, if you are involved with or know of forensic students actively engaged in forensic research or having unique forensic case analysis using any type of scanning microscopy, have your student(s) submit an abstract for consideration as a student paper.
Posters (both student and professional) are also encouraged!
Also presented at SCANNING 2000:
****** Scanning Microscopy in Forensic Science Short Course
Tuesday, May 8, 2000, 8:30am-4:30pm Instructors: S.F. Platek, USFDA-Forensic Chemistry Center, Cincinnati, OH; D.C. Ward, USDOJ - FBI, Washington D.C.; M.A.Trimpe, Hamilton Co. Coroner's Office, Cincinnati, OH, USA ;D.J. Ballantyne, RMCP, Ottawa, ONT, Canada
This short course is devoted to scanning microscopy analysis of forensic samples. Some of the specific topics to be covered include gunshot residue (GSR) analysis, particulate trace evidence analysis and food product/pharmaceutical tampering and counterfeiting. An intensive trace evidence section in forensic sample processing will be presented which includes collection, preparation, embedding, polishing, sectioning, mounting and micromanipulation of fine particles. Several atypical and/or new applications of scanning microscopy in forensic analyses including SEM/EDX and AFM will be illustrated. With the increased efforts of many laboratories being certified or moving toward certification by the American Society of Crime Laboratory Directors (ASCLD), some discussion will be related to sample/case processing and archiving as well as related laboratory procedures. Each section of the short course will be instructed by forensic scientists/microscopists, each a specialist in his respective area of expertise. Course format will include handouts as well as supplemental case histories as examples and a group question and answer session.
******
SCANNING 2000 http://www.scanning.org
SCANNING 2000, the Twelfth Annual International Scientific Meeting on Scanning Microscopies, will be held May 9-12, 2000, in beautiful San Antonio, Texas at the Four Points Sheraton Riverwalk North. Please make plans to join us for three full days (May 9-11, 2000) of forensic papers as well as other sessions in scanning microscopy including food contaminants and microsctucture, pharmaceuticals, digital imaging and analysis, 3-D microscopy and more..
******
Should you have any questions about the forensic symposium, short course or student papers, please contact.
S. Frank Platek US FDA - Forensic Chemistry Center Chairman, Forensic Symposium and Short Course SCANNING 2000 (513) 679-2700 (513) 679-2761 FAX fplatek-at-ora.fda.gov
******
Should you have any questions about SCANNING 2000, please contact the Foundation for Advances in Medicine and Science, Inc. (FAMS)
-at- FAMS, Inc. P.O. Box 832 Mahwah, NJ 07430-0832, USA
The ST-225 drive, which was extremely common in PC-XT's and clones around 1987-1990 is logically the same as the drive supplied by Link in the AN10000, but is a 5.25" drive rather than 3.5". In my AN10000 there was an unoccupied 5.25" drive bay, so it was the work of moments to install the drive. It formats up just like the Link-supplied drive. Since I discovered this, I don't let any old XT get scrapped without my first removing the ST-225 drive. I'm now on the second ST-225 in the AN10000.
I'm almost sure that *any* MFM hard drive of 20Meg or more would work, but the BIOS in the AN10000 will only recognise the first 20 Megs of it with the standard setup. If you can get an 80 Meg drive, there is a way to make the AN10000 recognise that (you have to change the jumpers on the drive, but it is so long ago I forget the details. It involves the AN10000 recognising the drive as DS0 rather than DS1, I think), but I haven't tried doing it. In fact, I discovered this when I bought a drive from Link and it was configured wrong, and the AN10000 thought it was an 80Meg drive!
I haven't had cause to investigate, but I assume that the original ExL's worked the same, but with an ST-251-1 40Meg MFM drive.
Cheers,
Tony.
} } Hi } I have an AN10000 EDX system. The hard disk has gone down. I usually use NEC } D3142 20Mb replacements. Sadly my supplier can no longer source this drive. } Does anyone have a suggestion for an alternative drive. } } Many thanks } } Chris } } } Chris Gilpin } Experimental Officer } Biological Sciences EM Unit } G452 Stopford Building } Oxford Road } Manchester } M13 9PT } phone +44 0161 275 5170 } Fax +44 0161 275 5171 } http://www.empgu.man.ac.uk } } }
Dear Petra, I cannot imagine why you would make a 3 mm. disc first. When I do carbon replicas, I just use the polished and slightly etched (in Nital) surface of a steel block of convenient size. Carbon-coat the block, score the carbon coat with a razor blade or scalple into 3 mm. squares, then immerse in Nital until the little carbon squares float free. Scoop these up with a copper TEM grid. This will provide a nice replica of the etched surface with the precipitates in place. By dissolving the entire specimen you may have collected too many precipitates. At 05:03 PM 12/15/99 +0100, you wrote: } } I tried for the first time to produce carbon extraction replicas of steel } containing several types of precipitates (TiC, TiS, TiN, ...). } I produced a disk of 3mm and thinned it electrolytically. On this specimen } I evaporated carbon and removed the steel disk with bathing it in Nital. } I come up with thin carbon layers with lots of precipitates on, but I am } quite sure that their distribution is not representative for their original } positions on the surface of the steel specimen. } Any tips and tricks how to handle this kind of preparation in detail? } } Petra
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
You can get the Schematics form Technotrade. I believe that they now service all of the Balzers equipment. They have been able to help me with my SCD 030. Give them a call at (603) 622-5011. Good luck with your unit. ______________________________________________ Roberto Garcia Senior Analyst, Metallography NC State University / Analytical Instrumentation Facility Campus Box 7531 Room 318 EGRC 1010 Main Campus Dr. Raleigh, NC 27695-7531 (919) 515-8628 (919) 515-6965 Fax rgarcia-at-unity.ncsu.edu http://spm.aif.ncsu.edu/aif ____________________________________________
} } Dear Microscopopists, } } I'm starting a new histology project for pathogenic invasion of cereal } grains } by fungi using GMA embedding. I've been using safranin & fast green for } lignin, but getting marginal results relative to paraffin. Does anyome } have a } good alternative stain or stain protocol for GMA/lignin demonstration? } } Please reply to: krueg001-at-tc.umn.edu } } Thanks for any help you can give. } } Darryl Krueger } University of Minnesota } Cereal Disease Lab }
Gib Ahlstrand Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu http://biosci.umn.edu/MIC/consortium.html
Uri: Endless suppliers carry graticules/reticles including ProSciTech and I think that we have the one that you are looking for. Its cat. no S8018, check the online. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Saturday, December 18, 1999 1:07 AM, uri [SMTP:uri-at-watson.ibm.com] wrote: } } Brian Gortney says: } } Jennifer: } } Try Edmund Scientific for an inexpensive solution at } } www.edmundscientific.com } } I seems that Edmund's has only *reticles*, but not *graticules*? } I.e. measuring things but not counting ones? } } I'd be happy to be proven wrong here - I need a graticule } myself (21mm disk, 5mm of squares 10x10). } -- } Regards, } Uri uri-at-watson.ibm.com } -=-=-=-=-=-=- } {Disclaimer} }
If you have already responded to the following announcement a few days ago, that means your package is already on its way and it should be arriving soon! If you have not responded to this before, please pay attention to it now. This is very important!!!
Before you know about this 'Important Announcement', you must first read the following 'Editorial Excerpts' from some important publications in the United States:
NEW YORK TIMES: "In concluding our review of Financial organizations to effect change in the 90's, special attention should be called to a California based organization, 'WORLD CURRENCY CARTEL'. Members of this organization are amassing hundred of millions of dollars in the currency market using a very LEGAL method which has NEVER been divulged to the general public. While their purpose is not yet known, their presence has most certainly been felt".
NBC NIGHTLY NEWS: "Members of 'World Currency Cartel', who always keep a low profile, are considered to be some of the most wealthiest people in North America".
More excerpts later, but first let us give you this very "IMPORTANT ANNOUNCEMENT":
We are glad to announce that for the first time and for a very short period of time, WORLD CURRENCY CARTEL will instruct a LIMITED number of people worldwide on 'HOW TO CONVERT $25 INTO ONE HUNDRED OF LEGAL CURRENCY'. We will transact the first conversion for you, after that you can easily and quickly do this on your own hundreds or even thousands of times every month. TAKE ADVANTAGE OF THIS "SECRET FLAW"!
It is even more explosive than we have yet disclosed. While currency does fluctuate daily, we can show you 'HOW TO CONVERT $99 INTO $588 AS MANY TIMES AS YOU WANT'. That means, you will be able to EXCHANGE $99, AMERICAN LEGAL CURRENCY DOLLARS, FOR $580 OF THE SAME. You can do this as many times as you wish, every day, every week, every month. All very LEGAL and effortlessly!
It takes only 5 to 10 minutes each time you do this. You can do this from home, office or even while traveling. All you need is an access to a phone line and an address. Best of all, you can do this from ANY CITY ON THIS EARTH!!!
Again, we must reiterate, anyone can do this and the source is NEVER-ENDING. For as long as the global financial community continues to use different currencies with varying exchange rates, the "SECRET FLAW" will exist.
As we said earlier , we will do the first transaction for you and will show you exactly how to do this on your own, over and over again!
The amount of exchange you would do each time is entirely up to you. Working just 2 to 10 hours a week, you can soon join the list of Millionaires who do this on a daily basis many times a day. The transaction is so simple that even a high school kid can do it!
We at the World Currency Cartel would like to see a uniform global currency backed by Gold. But, until then, we will allow a LIMITED number of individuals worldwide to share in the UNLIMITED PROFITS provided for by the world currency differentials.
We will espouse no more political views nor will we ask you to do so. We can say however, that our parent organization, CILS, benefits greatly by the knowledge being shared, as we ourselves, along with YOU, benefit likewise. Your main concern surely will be, how you will benefit.
As soon as you become a member, you will make transactions from your home, office, by telephone or through the mail. You can conduct these transactions even while traveling.
Unlike anyone else, we will assure you great financial freedom and you will add to our quickly growing base of supporters and join the list of MILLIONAIRES being created using this very "SECRET FLAW" in the world currency market.
There is a one time membership fee of only $195. BUT, if you join within the next 10 days, you can join us for only $25 administrative cost. Your important documents, instructions, contact name/address, phone number and all other pertinent information will be mailed to you immediately. So take advantage of our Anniversary date and join us today.
(If you are replying after the next 10 days, you must pay $195.00 for the membership fee. NO EXCEPTIONS, and no more E-mail inquiries please).
Upon becoming a member, you promise to keep all infos CONFIDENTIAL!
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Should you choose to cancel your membership for any reason, you must return all papers/documents for a refund within 30 days.
1...Please write your name & mailing address VERY CLEARLY on a paper 2...Below your mailing address, please write your E-mail address 3...At the top left hand corner, please write the words "NEW MEMBER" 4...Attach a CHECK for $25 + $10 for the shipping and handling of documents (TOTAL = $35.00) PAYABLE TO "NDML" and FAX it to:
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(Note: We are ONLY accepting CHECK-BY-FAX as a form of payment at this time. We WILL be able to cash the check you send us by fax, you do not need to mail us a check. If your check is dark, please PRINT ALL OF THE INFORMATION ON THE CHECK ONTO THE PAPER YOU ARE FAXING US so that it is clearly legible!) Please allow 2-4 weeks for delivery. No shipments will be made until the check has cleared.
WALL STREET: "A discreet group of Americans, operating under the guise of World Currency Cartel have recently begun making rumbles in world finance market. While at this time, their game is not completely known, they certainly will be watched by those making major moves in the currency contracts".
FINANCIAL WEEK: "Watch them, monitor them, extract their knowledge and try to become one of them. That is the soundest financial advice we could give to anyone".
NATIONAL BUSINESS WEEKLY: "While this reporter has been left in the cold as to its method of operation, we have been able to confirm that 'World Currency Cartel' and its members are literally amassing great fortunes overnight".
To be removed from our list, simply click "reply" and put the words "Remove Currency" in the subject line. Warning: If you do not put the words "Remove Currency" in the subject line, you will not be removed. The process is automated.
Postdoctoral Position, Institute of Condensed Matter Physics, University of Lausanne, Switzerland: The Group of Physics of Living Matter under the direction of Prof. G. Dietler is offering a postdoctoral position in the area of cryogenic (low temperature) scanning probe microscopy (SPM). The cryo-SPM is currently under construction and further modifications are needed at the present time. The design of the cryo-SPM will allow it to be used for several applications of research after completion. Candidates with a Ph.D. and experience in the construction of instruments for cryogenics and/or ultra high vacuum (UHV) SPM are preferred. Good candidates with a Ph.D. and a background in construction of other types of instrumentation used for vacuum or microscopy research will also be considered. To apply for the position, please send your CV with a brief description of your research experience, a list of publications, and the names and contact information of at least 3 references to:
Prof. G. Dietler Institut de Physique de la Matiere Condensee (IPMC), BSP Universite de Lausanne CH-1015 Lausanne Switzerland Tel: 41 21 692 3663 (off.) 3682 (off.) 3660 (sec.) Fax: 3635 Email: Giovanni.Dietler-at-ipmc.unil.ch http://www.unil.ch/ipmc/docs/gd/home.html
Applications sent by email are preferred in the interest of time.
************************************************ Robert J. Derby New Mexico Institute of Technology Socorro, N.M. Phone - 505-835-5866 E-mail - rjderby-at-excite.com derby-at-nmt.edu ************************************************ First, Happy Holidays to all, and thanks for all the help in the past. Now my question... We have just gotten a JEOL 6100 SEM, still being setup. I would like to know if anyone has gotten a signal out to a computer (in my case a Mac). I know of a RS-170 out, but I would like it to be a digital signal. Has anyone hooked up a 6100 to a computer and if so what did you do? A cheap fix would be best without spending 5-10K on a Scion framgrabber. Thak you, and everyone have a Happy New Year.
_______________________________________________________ Visit Excite Shopping at http://shopping.excite.com The fastest way to find your Holiday gift this season
At 05:40 PM 11/30/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I use CDQ-74SZA, 10 pack in jewel cases with no problems for over two years. I still prefer the Memorex silver.
The worst choices are the bulk spindles of green or blue.
I am curious to here about various TEM staining approaches, in particular about vapor staining via OsO4 crystals.
Whatrecommendations can you provide for a OsO4 staining apparatus to stain thin sections or pre-microtome-hardening of polymeric samples?
Whatis the difference between the effectiveness of a 2% aqueous OsO4 solution and OsO4 crystals?
Whatis the best procedure to neutralize the stain after use? Is the recommended procedure for neutralizing an aqueous OsO4 solution as suggested in the EMS catalog (twice the volume of corn oil) also applicable to solid OsO4 crystals?
I am unhappy with our present staining apparatus. I suspect that the OsO4 is leaking. A paper scrap with a fingerprints turns gray after a few month= s in the hood where the staining apparatus is located. Secondly, I am not certain how to =94kill=94 the remaining OsO4 in the vapour-phase after our=
samples have been stained.
Any recommendations addressing my above questions are appreciated. Any additional comments concerning the staining of polymers such as the block copolymers of PS/PB and PS/PI are also welcome.
Qualifications (education, certification, language, etc.) and Experience required: A candidate with a BS or MS or PHD degree in polymer science, material science or chemistry is preferred with some prior experience in electron microscopy. Good written and oral communication skills and the ability to work both independently and in a team environment are extremely important.
Job Overview:
The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D Analytical Science Laboratory has one professional level full time opening for Polymer Microscopist in Dow's Freeport, Texas, location. The primary responsibilities include working with partners to support research projects involving new and existing products in Dow's polymer businesses.
Key responsibilities will include:
1. Extensive problem solving. 2. Microscopy preparation technique experience including ultramicrotomy and cryo-ultramicrotomy. 3. Operation of light, transmission, and scanning electron microscopes. 4. Interpretation of images. 5. Documentation and communication of work results. 6. Compliance with safety and quality programs. 7. Active member of project and SMX work teams.
Interested: Please e-mail or send your resume and cover letter, with reference to this ad to: Email: R&D-at-Dow.com or The Dow Chemical Company, Workforce Planning 005855, P. O. Box 150, Plaquemine, LA 70765. E-mail respondents must list Job 005855USA and their last name as the first and second items on the Subject line. Only those selected for an interview will be contacted. Only U.S. citizens or aliens who are authorized to work in the United States will be considered for employment.
We are an equal opportunity employer and offer a competitive compensation and benefits package including 401k, stock purchase, tuition reimbursement and performance incentives. The Dow Chemical Company is the fifth largest chemical company in the world with annual sales of US$20billion. Dow manufactures and supplies chemicals, plastics and agricultural products for customers in 164 countries and employs approx. 43,000 people worldwide. For more news and information about Dow, please visit our web site at www.dow.com.
Robert C. Cieslinski Microscopy & Microanalysis (517) 636-6875 email: rccieslinski-at-dow.com
We are currently soliciting "best offers" on our Hitachi S-800 FEG SEM. It has a GW electronics microchannel plate BSE detector and a Macintosh-based 4pi Analysis digital image acquisition system. Purchased in 1988.
Please contact me with any questions you many have.
Sincerely,
Anne E. Huber
_______________________________________ Anne E. Huber Ph.D., Instrument Analyst Materials Science and Engineering Dept. The University of Michigan 2300 Hayward St. Ann Arbor, MI 48109-2136 ahuber-at-umich.edu (734)764-3357 _______________________________________
} } I have a glass (leaded glass) tube, about 0.5 cm in diameter. The inside } } wall of the tube has been leached leaving behind a porous silica network } } with pores about 500 nm in size. The thickness of the leached layer is } } estimated to be about 400 nm. We would like to prepare cross sections to } } look at the glass/leached layer interface ... Has anyone tried microtomy } } with this type samples?.
} } Jordi Marti } } Dear Jordi } Conventional microtomy won't work on this kind of hard brittle } friable material.
} Chris Jeffree
Jordi -
Chris is being a bit too negative about microtomy of coatings on glass. It's demanding, but Phil Swab teaches how to do it annually in the Ventana - RMC Materials Microtomy workshop. You can contact him for an opinion at phil.swab-at-depsci.com.
Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html
Rick Felten-at-MACDERMID 12/20/99 03:42 PM Has any purchased or demonstrated a RJ Lee PSM-300 and would like to share their opinion about the quality of this scope in a conventional SEM mode? Thanks Ric
I have acquired a (used) Denton DV515 coater. I want to refresh the pump oil and santovac 5 before I reassemble it.
But the manual omits to say what the volume of santovac should be in the diff pump.
Does anyone remember?
Dr. Mel Dickson, Deputy Director, The Electron Microscope Unit, Adjunct Associate Professor, School of Microbiology & Immunology The University of New South Wales Sydney, Australia. Phone +612 9385 6383 Fax +612 9385 6400
You can use the clasicc minerology and materialography techniques;
1. include glas tube in the liquid fluorescence resin in the vacumm chamber,
2. cut this sample on the parts by automatic cut-off machine with diamond disc,
3. grinding and polishing this sample
4. put the sample on the optical microscopy stage and say - yes, it is not a problem,
(ask about this problem friends from materials science department or mineralogy or metallography, or read the book Vander Voort - metalography nad principles,)
best regards
Krzysztof Jan Huebner
{hubner-at-IOd.krakow.pl} :-)
Foundry Research Institute ul Zakopianska 73 telefon (0-12) 2618111 wew 356 30-418 Krakow, PL faks (0-12) 2660870
On Mon, 20 Dec 1999, Caroline Schooley wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } } I have a glass (leaded glass) tube, about 0.5 cm in diameter. The inside } } } wall of the tube has been leached leaving behind a porous silica network } } } with pores about 500 nm in size. The thickness of the leached layer is } } } estimated to be about 400 nm. We would like to prepare cross sections to } } } look at the glass/leached layer interface ... Has anyone tried microtomy } } } with this type samples?. } } } } Jordi Marti } } } } Dear Jordi } } Conventional microtomy won't work on this kind of hard brittle } } friable material. } } } Chris Jeffree } } Jordi - } } Chris is being a bit too negative about microtomy of coatings on glass. } It's demanding, but Phil Swab teaches how to do it annually in the Ventana } - RMC Materials Microtomy workshop. You can contact him for an opinion at } phil.swab-at-depsci.com. } } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html } Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html } } } }
} Why are you making a 3mm disk and then thinning it? That looks like extra } work.
You were not the only one to ask this question. In the beginning, I did it because I wanted to look first at the original specimen. (This is why I am sure the distribution of the particles on the replica is not representative.) Later on, I did it because it did not come to my mind to change the procedure :)
Offline, I received several protocols how to produce a replica from a bulk piece of steel. They contain a lot of helpful details that will help me certainly to produce a good specimen.
Thanks to all who took the time to answer my question,
Petra
At 17:26 15.12.99 -0500, you wrote: } Why are you making a 3mm disk and then thinning it? That looks like extra } work. } } You can polish a bulk sample of your material, etch it as Tony Garratt-Reed } suggests, and coat the sample with carbon which "grabs" the particles. Now } slightly score small rectangular sections on your sample so that your } etchant can get under the carbon layer and attack the steel. Float them off } after sufficient etching and collect them on grids. For security, you can } put another coating on the top of the exposed particles to "seal them in". } } -Scott } } } Scott D. Walck, Ph.D. } PPG Industries, Inc. } Glass Technology Center } Guys Run Rd. (packages) } P. O. Box 11472 (letters) } Pittsburgh, PA 15238-0472 } } Walck-at-PPG.com } } (412) 820-8651 (office) } (412) 820-8161 (fax)
-------------------------------------------------------------- Dr. Petra Wahlbring Centre de Recherche Public - Gabriel Lippmann Laboratoire d'Analyse des Materiaux (LAM) 162a, av. de la Faiencerie L-1511 Luxembourg tel. +352-466644-402 fax +352-466644-400 e-mail: petra.wahlbring-at-crpgl.lu Visit our WWW site! http://www.crpgl.lu/~wahlbrin
Good idea, Caroline. I'll volunteer to coordinate entries if that will be helpful. My experience in product development for Polaroid and in publishing for this company may be useful. I'll put the suggestion on the table and wait to hear what you decide.
Elinor Solit The Cambrex Group, Publishers of The Microscope Book
On Thu, 16 Dec 1999, Caroline Schooley wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I think that the printer comparison suggested for the Philadelphia meeting } is a great idea; I have two comments and a related question. The comments: } 1) Nestor & John have too much to do as it is; someone should volunteer to } help with this project. } 2) The question about printing paper/ink structure suggests an excellent } topic for someone who wants to apply for a Professional Technical Staff } award to attend the meeting (see the meeting announcement, pg. 13). } And the question: I've learned (the hard way) about the fragile, sticky } surface of inkjet prints. Has anyone tried the new Gepe Inkjet Fixative } yet? Does it work well? } } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html } Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html } } }
I am pleased to bring to your attention the following SEM technician position at Motorola's Process and Materials Characterization Laboratory (PMCL I encourage all persons interested to respond ASAP.
Position: SEM Technician/S9001P Employer: Motorola-Semiconductor Products Sector's Process and Materials Characterization Laboratory (PMCL) Location: Mesa, AZ Employment type: Full-time Employment status: Full-employee (non-contractor) Number of Positions: 1 Shift: N1-compressed (6:00 P.M.-6:00 A.M. E/O Saturday, Sun-Tues) or N2 (6:00 P.M.-6:00 A.M. Wed-Fri, E/O Saturday) Relocation: Available
Duties/Responsibilities: Experience in Scanning Electron Microscopy techniques including basic operation, specimen preparation, maintenance, and troubleshooting of SEM's. Work effectively as a team player in multiple projects providing routine and non-routine SEM analysis in support of semiconductor product manufacturing. Exposure to many different types of processes and technologies, and working knowledge of FIB and EDS are a plus.
Specific knowledge: AA degree preferred. A higher or lower classification will be established depending upon qualification and experience.
Contact info: Send resume or questions via email to Brian_Wajdyk-at-email.mot.com or fax to 480-655-4316 C/O Brian Wajdyk. -- ******************************************************************** Brian Wajdyk Team Leader / Electron Microscopist (FESEM, EDS, SAM) Motorola - Process and Materials Characterization Laboratory (PMCL) 2200 W. Broadway Rd., Mesa AZ 85202 Mail Drop: M360 Tel: 480-655-4337 Fax: 480-655-4316 Email: brian_wajdyk-at-email.mot.com Pager: 1-800-313-5960 ********************************************************************
I'm comfortable with handing everything on a poster board to be located in the Computer Workshop area at the M&M 2000 meeting.
We have done similiar in the past, except that things were layed out on a table. The key is the documentation and using the "standard test image". As we approach the time of the meeting I'll post a call for prints on the Listserver. Interested people can then download the standard image print out the image and bring it to the meeting along with a " information form" which we have used in the past (i.e. type of printer, ink, time to print etc....). For those that want to participate but will not be attending they can just mail the print and the form to me and I'll carry them across.
I've had a few volunteers who said they would help and I'll contact them off line. Basically I would ask them to come by the Computer Workshop and organize the Poster Board to hang all the prints and documentation. It will be a few hours work at most (hanging things up at the start/during the meeting and taking down at the end.)
Nestor Your Friendly Neighborhood SysOp & the M&M Computer Workshop Co Organizer (with John Mansfield).
================================================================== Nestor J. Zaluzec Materials Science Division Building 212 Argonne National Lab 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov ================================================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ==================================================================
The box said "This program requires Win 95/98/NT or better..." so I bought a G3 Mac
Since Nestor's got me back able to send messages to the list again, I have a question for you materials people out there. What would be the source of small (0.02mm x .001mm) rods of calcium/silicon/aluminum in plate steel samples? The steel is a little old, made in the Harland & Wolfe Shipyard, Belfast, Northern Ireland in 1910-1912 (yes, it's from R.M.S. Titanic). At first, I thought them to be biological, possibly sponge spicules, but since then I've found some deeply embedded in the steel, not just on the surface, and spicules are normally either siliceous or carbonate, not both, as my trusty EDS detector tells me. The rods themselves are very smooth on the surface, normally perfectly straight, and when you see one "on end" they appear to have a radial kind of internal structure. Would they perhaps be some kind of remnant from the lime used in the smelting? Or some kind of secondary mineralization? Any thoughts would be appreciated. Oh, and Season's Greetings, by the way....
F.C. Thomas MicroAnalysis Facility Geological Survey of Canada (Atlantic) Bedford Institute of Oceanography Dartmouth, Nova Scotia Canada B2Y 4A2
} I have acquired a (used) Denton DV515 coater. I want to refresh the pump } oil and santovac 5 before I reassemble it. } } But the manual omits to say what the volume of santovac should be in the } diff pump. } } Does anyone remember? }
Dear Mel, No, but if there is a dipstick near the bottom, that should at least give you a clue, and in the best of worlds there would be a "full" mark with the volume indicated and an "add oil" mark. Yours, Bill Tivol
the power supply in my 5500 is on the fritz. does anyone know what the replacement # would be? i think its a todd power supply (which is now condor?). also, are there a pots for voltage adjustment on the unit? it may be that simple too....
thanks!
brian
**************************************************************** Brian McIntyre Electron Microscopy Lab Institute of Optics University of Rochester Rochester, NY 14627
716-275-3058 716-244-4936(fax)
"You may get to the top of the ladder of success only to find its been leaning against the wrong wall" A. Raime
I am posting this for a friend who tells me there is a JEOL 100B TEM in working condition on this campus, in a room needed for other purposes. If anyone is interested in having this scope for use or parts, please let me know, and I'll pass the message on to the right people.
Thanks. Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 tindallr-at-missouri.edu http://www.biotech.missouri.edu/emc/
The use of stereo anaglyph (red-green or red-blue) images for visualization of AFM images is reported in a paper in the current (December 1999) isssue of the Journal of Microscopy. A much broader range of possibilities for using stereo for surface imaging exists, and we've just submitted a paper illustrating a variety of modes that combined stereo presentation with rendered surfaces, perspective corrected views, etc. The possibility of recovering surface elevation information from stereo images (e.g., from SEM) is also demonstrated. A preprint of the paper (not yet reviewed) in pdf (acrobat) format can be downloaded by anyone interested from http://members.AOL.com/DrJohnRuss/Stereo.pdf; the paper includes information on the software used to generate the images.
John Russ Materials Science and Engineering Dept. North Carolina State University Raleigh, NC 27606
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Hello everyone, Since that snow is a hexagonal, positive uniaxial crystal with dendritic and basal tablet habit, and with n (omega) =1.309 (D-line) and n (epsilon) =1.3147 (D-line) and delta n = 0.005, how could you fail to have a Merry Christmas and a Happy New year!
Question: We are using our SEM to image the sphere sample surface and analyse the diamond density ( No. per area) and height on the surface. Is there any software can do this analysis.
AOL's server appears to be case sensitive. The correct URL for John's paper is:
http://members.AOL.com/DrJohnRuss/stereo.pdf
Tony.
At 05:09 PM 12/21/1999 EST, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
We have a lot of data from our mass spec. stored on cdr , would someone email me with the details of cdr manuf. number etc. Since they use several different manuf. they would like to know which ones might be a problem. Terry Ellis email : tellis2-at-hallmark.com this is not a joke Hallmark does have a research lab
I tried to e-mail you directly, but it got kicked back for some reason. I have a Denton DV-502. For my machine, and possibly yours as well, Denton offered 2 different size diffusion pumps: 3-1/4" & 5-3/4" diameters. Mine has the 3-1/4" pump, which is model DP-250. It takes 100cc of fluid - the factory filled it with Dow Corning DC-704, but I also prefer Santovac 5 as the problem of a possible artifact Si peak is eliminated. If you have the larger pump, try contacting Denton. I have 2 numbers for them: 609/424-1012 and 856/439-9100, the latter being for parts & service. Hope this helps.....
The Electron Microcopy Analysis Center (EMAC) at West Chester University in Pennsylvania has an opening for a full-time electron microscopy technician. The EMAC is primarily used by geologists, biologists and chemists. The successful applicant will have a minimum of a Bachelor=B9s degree and 3 years experience in the operation and maintenance of transmission and scanning electron microscopes (including some service experience), the instrumentation routinely used in specimen preparation, and proficiency in photographic and darkroom techniques. The ability to operate an X-ray diffractometer, X-ray fluorescence spectrometer or confocal microscope is desirable. Responsibilities will include daily operation and maintenance of the EMAC including user training. The successful applicant must demonstrate the necessary organizational, management and communication skills to efficiently operate the EMAC. Applicants should submit a cover letter, resume and three letter of recommendation to the Department of Human Resources, 210 Carter Drive, West Chester University, West Chester, PA, 19383. Applications must be received by March 1 2000. Applicants must successfully complete the interview process to be considered a finalist. AA/EOE. Women and minorities are encouraged to apply.
Dr. Giovanni Casotti PhD. Department of Biology West Chester University West Chester, PA, 19383 email:giovanni-at-bio.wcupa.edu or: gcasotti-at-mail.wcupa.edu ph: (610) 436-2856 fax: (610) 436-2183
The Electron Microcopy Analysis Center (EMAC) at West Chester University in Pennsylvania has an opening for a full-time electron microscopy technician. The EMAC is primarily used by geologists, biologists and chemists. The successful applicant will have a minimum of a Bachelor’s degree and 3 years experience in the operation and maintenance of transmission and scanning electron microscopes (including some service experience), the instrumentation routinely used in specimen preparation, and proficiency in photographic and darkroom techniques. The ability to operate an X-ray diffractometer, X-ray fluorescence spectrometer or confocal microscope is desirable. Responsibilities will include daily operation and maintenance of the EMAC including user training. The successful applicant must demonstrate the necessary organizational, management and communication skills to efficiently operate the EMAC. Applicants should submit a cover letter, resume and three letter of recommendation to the Department of Human Resources, 210 Carter Drive, West Chester University, West Chester, PA, 19383. Applications must be received by March 1 2000. Applicants must successfully complete the interview process to be considered a finalist. AA/EOE. Women and minorities are encouraged to apply.
Dear Frank, If these are oxides or sulfides, they sound like slag inclusions from the original steel-making, although if there are very many of them it would indicate a poor quality steel. Oh well, it wasn't a metallurgical failure. These inclusions can assume a variety of forms, depending on the treatment and manipulation of the steel. If you can ask a failure analysis person to look at the pictures, they can probably tell you. At 02:10 PM 12/21/99 -0400, you wrote: } Since Nestor's got me back able to send messages to the list again, I have } a question for you materials people out there. } What would be the source of small (0.02mm x .001mm) rods of } calcium/silicon/aluminum in plate steel samples? The steel is a little old, } made in the Harland & Wolfe Shipyard, Belfast, Northern Ireland in } 1910-1912 (yes, it's from R.M.S. Titanic). At first, I thought them to be } biological, possibly sponge spicules, but since then I've found some deeply } embedded in the steel, not just on the surface, and spicules are normally } either siliceous or carbonate, not both, as my trusty EDS detector tells } me. } The rods themselves are very smooth on the surface, normally perfectly } straight, and when you see one "on end" they appear to have a radial kind } of internal structure. Would they perhaps be some kind of remnant from the } lime used in the smelting? Or some kind of secondary mineralization? } Any thoughts would be appreciated. } Oh, and Season's Greetings, by the way.... } } F.C. Thomas
Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
For 5-.." DP on DV-502 (which is Varian's M-6, actually) 250 ml of oil is fine. I am using Santovac-5. I don't know anything about DV-515. You have to call Denton.
Good luck. Sergey
At 10:29 AM 12/22/99 -0700, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Sergey Ryazantsev Ph. D. UCLA School of Medicine Department of Biological Chemistry Box 951737 Los Angeles, CA 90095-1737
We recently started using a small tip transfer pipet to process tissue for TEM. Students especially find them a lot easier to use and lose a lot less of their tissue. We ordered them from PGC Scientifics {www.pgcscientifics.com} They are listed under the "Disposable Plastic Transfer Pipets" section and I think the cat.# is 71-5199-73. These have a 1 ml capacity . The tip is about 1 mm diameter. There are larger volume small tip pipets .
The Usual Disclaimer: I have no connection with PGC (in fact they didn't even return an email question!) Other vendors may stock this item.
Mike Baxter Lehman College Bronx, NY mykkb-at-juno.com ___________________________________________________________________ Why pay more to get Web access? Try Juno for FREE -- then it's just $9.95/month if you act NOW! Get your free software today: http://dl.www.juno.com/dynoget/tagj.
Brian McIntyre mailto:mcintyre-at-optics.rochester.edu Sr. Engineer lab: 716-275-3058/4875 River Campus EMLab fax: 716-244-4936 University of Rochester Rochester, NY 14620
"The most important thing a father can do for his children is to love their mother." - Unknown
To clear up a lot of confusion; there is a model DV515 that uses a unique rapid heating diffusion pump. This pump takes only 100cc of oil. This unit takes Santovac 5 ONLY as I recall. There are special considerations for this pump design (types of o-rings, heaters), you should talk with the manufacturer.
The standard 3" pump DV 502 uses DC704 oil, 100cc. The large 6" pump uses 250cc. Santovac 5 can be substituted but pumps slower since it is made for a higher heat.
Contact Mr. Jim Falco at Denton if you want information from the source; phone 865-439-9100, fax 856-439-9111.
I do not have a financial interest in Denton Vacuum (but did for 20+ years).
Steve Miller Ventana Medical Systems, Inc. www.Ventanamed.com Phone: 800-227-2155, ext 2753
Image Pro Plus and MetaMorph, among others will do this. Please note that I have no financial affiliation with either company. Wanda Shotsberger Harris Methodist Hospital Fort Worth Texas ---------- } From: "feir-at-bsci.com"-at-Sparc5.Microscopy.Com To: Microscopy-at-Sparc5.Microscopy.Com -----------------------------------------------------------------------.
Email: feir-at-bsci.com Name: Ray Fei
Question: We are using our SEM to image the sphere sample surface and analyse the diamond density ( No. per area) and height on the surface. Is there any software can do this analysis.
Just wanted to thank all of you who made suggestions about field microscope= s for my entomologist friend. The information is now in her hands to deal = with before the next field season starts!
Holiday greetings!
Paula.
Paula Allan-Wojtas Research Scientist, Food Microstructure Agriculture and Agri-Food Canada Atlantic Food and Horticulture Research Centre Kentville, Nova Scotia, Canada B4N 1J5
Suggest you contact a long-time friend and colleague, Barry Fookes, now at UCF: 407-823-6205. Tell him I sent you.
While head of the Experimental Techniques Center at Brunel U (just outside of London), he used to analyze all sort of steels and may have some insight. Sorry, but I am on the road and don't have his email with me.
Best of luck .... and Happy Holidays to all! Barbara Foster Microscopy/Microscopy Education 125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
At 04:13 PM 12/22/99 -0800, Mary Mager wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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Email: guru-at-biosci.umn.edu Name: Guru R Thuduppathy School: University of minnesota Question: Hi
I would like to know what a capillary microscope is. What I know about it is that it is used in diagnosis for arthritic ailments. Could you provide me scientific details, probably a description of it, whether it looks like a standard microscope or rather like an opthalmoscope.
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Does anyone know where to but tacky wax or a similar substitute. I've heard glue from tape will also aid in serial sectioning, but I was looking for this alternative in particular. Any help is much appreciated. Thanks. Linda Chicoine
Linda, We got our Tackiwax about 5 years ago from one of the major scientific supply houses, probably Fisher. But it is (or was) made by a firm called Boekel Industries, who are (or were) at Philadelphia, PA. Hope this helps, Tobias Baskin
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Once again, a question on behalf of a colleague. Does anyone know of a stain that's is specific (or at least moderately so) for cellulose? Cellulose microfibrils, to be exact. We have located references for cellulose stains, but the specificity information hasn't been there.
Thanks, as usual, and best wishes.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 tindallr-at-missouri.edu http://www.biotech.missouri.edu/emc/
Greetings, The usual stains in the LM are calcofluor white (also known by other names such as citifluor) and congo red. These stains definitely stain other polysaccharides. THey cannot be used to identify cellulose in an unknown sample. What can be done with both stains is to take advantage of dichroism with congo red, or polarized florescence with calcofluor. Becuase the stains bind in an oriented way to the microfibrils, the absorption or emission properties of the dyes become sensitive to the polarization state of the incident light.
Much more specific is to use a probe make from the cellulose binding site of a cellulase. I have seen this done at the EM level with conjugation to gold, but in principle one should be able to prepare say CY-3 conjugated cellulase.
Hope this helps, Tobias
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I do not know of a cellulose specific stain, but ..........Graffs "C" stain could work depending on your objective. It is an informal paper industry standard for differentiating chemical treatments. It can be purchased from Integrated Paper Services, Inc. (Madison Wisconsin). They are listed at http://www.mwrn.com. You could also purchase it from Aldrich-Sigma. It is light sensitive and has a limited shelf life. Ask for Mr. Rantanen....he might know of a cellulose specific stain if there is one. -----Original Message----- } From: Tobias Baskin {BaskinT-at-missouri.edu} To: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}
We have a Hitachi S-800 FESEM that is mostly used for biological specimens. However, recently we have had a number of planetary geologists looking at sections of meteorites mounted on epoxy resin. About the time they started using the scope, we started having a number of problems that suggest that we are getting some outgassing and contamination. I'm not surprised; this happened before when looking at fish ear bones in similar resin, despite the resin manufacturer's clain their product was completely stable in the high vacuum and under the beam.
My question is for those of you who routinely look at such samples: What do you do to minimize the potential problems? Hold the samples in a vacuum for some period of time before putting them into the scope? Paint the exposed epoxy areas with carbon paint or similar? For the biological samples we only require that the specimens be held over dessicant overnight, but I suspect that more heroic measures must be taken for these samples.
Happy New Year to all!
Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
The problem of dealing with the evolution of contaminating materials from plastics used to mount metallurgical, ceramic and mineralogical specimens is discussed on pp.75 & 76 of my book, 'Vacuum Methods in Electron Microscopy' (see http://www.2spi.com/catalog/books/book48.html for a description).Related topics also discussed are gas evolution from leaks, construction materials, specimen materials, and from cleaning reagents and procedures.
Contamination from mounting polymers can indeed be a very vexing problem, especially for SEMs that have FEGs and must operate with a relatively good vacuum in the specimen chamber.
Basically, what we found, after a number of episodes of very serious contamination, was that it is necessary to be sure that the mounting polymers are mixed carefully and thoroughly, so that the correct relative amounts of polymer and hardener are used, and so that these components are thoroughly intermingled. Then we found it to be necessary to be sure that after they are mounted the specimens are allowed to stand for a long enough period (at least 24 to 48 hours) to ensure that the mounting polymer is completely polymerized (moderate heating can sometimes be used to accelerate the polymerization reaction - even 15 or 20 degree increase can have a significant effect). Finally we ended up requiring that after curing all such samples had to be pumped overnight in a chamber of the type that is used to evacuate photographic film before it is placed into an electron microscope.
Such procedures did not totally eliminate the problen, but reduced it to a level where we could operate for a month or more before contmination built up to the point where cleaning of the chamber and apertures became necessary.
Wilbur C. Bigelow, Prof. Emeritus Materials Sci. & Engr., University of Michigan Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-764-3321
(BTW, Nestor, the first post of this got kicked back because I used Y2K in the subject. There are times we may need to discuss it, as described below.)
Although I do not currently use a PDP-11 in my EDS system, I do still have our old PDP-based Kevex system around, and I notice that it does not accept dates beyond 1999. It will roll over into 2000, but if a date has to be entered afresh, it will not be accepted by my version of RT-11.
Checking some old PDP documentation, I see that the PDP is only setup to handle a span of 32 years under the versions of RT-11 and TSX that I have. They allocate five bits to the year part of the date and start with 1972. They must thus end with 2003. They will roll from 2003 back to 1972 if you try to push them. There are more recent versions of RT that will support later dates;however, I don't know how easy they are to come by. I also don't know if there would be any trouble in incorporating them into these EDS systems - hopefully not, but it is hard to say for sure.
I have messed around a little with writing a program that would set the date beyond 1999. Dates, as in directories, will show up in the form of 01-JAN-100, but it would probably be adequate. The program is in crude form now and I would like to refine it a bit more. I could then provide a copy to you or anyone else that needs one.
Of course, if someone else has already tackled this issue, or if no one is still using a PDP-based system (hard to believe), then I can better spend my time on other tasks. Please contact me if you would be interested in such a program.
---------------------- Warren E. Straszheim Materials Analysis and Research Lab Iowa State University 23 Town Engineering Ames IA, 50011-3232
I just received this notification from the Society for Analytical Chemists of Pittsburgh. Perhaps it will provide an important start for a new chem prof who has an interest in expanding the use of microscopy and/or in walking across the new bridge between microscopy and spectroscopy:
"Twenty-first annual Analytical Chemistry Starter Grant Award" The society for Analytical Chemists of Pittsburgh will award one grant of $20,000 to an assistant professor in the field of analytical chemistry. The purpose of this grant is to encourage high-quality, innovative research by a new analytical chemistry professor and to promote the training and development of graduate students in this field. Assistant professors who have accepted a US college or university appoint since December 31, 1996 are eligible. Application forms available from: James Chadwick, Chairman Starter Grant Committee Society for Analytical Chemists of Pittsburgh 200 Penn Center Blvd., Suite 332 Pittsburgh, PA 15235 Ph: 1-800-825-3221, Xt. 208 Fx: 412-825-3224
Deadline for application receipt: February 29, 2000 Award winner announced: May 1, 2000
Best regards and welcome to the new millennium! Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 08:44 PM 12/30/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } (BTW, Nestor, the first post of this got kicked back because I used Y2K in the subject. There are times we may need to discuss it, as described below.) } } Although I do not currently use a PDP-11 in my EDS system, I do still have our old PDP-based Kevex system around, and I notice that it does not accept dates beyond 1999. It will roll over into 2000, but if a date has to be entered afresh, it will not be accepted by my version of RT-11. } } Checking some old PDP documentation, I see that the PDP is only setup to handle a span of 32 years under the versions of RT-11 and TSX that I have. They allocate five bits to the year part of the date and start with 1972. They must thus end with 2003. They will roll from 2003 back to 1972 if you try to push them. There are more recent versions of RT that will support later dates;however, I don't know how easy they are to come by. I also don't know if there would be any trouble in incorporating them into these EDS systems - hopefully not, but it is hard to say for sure. } } I have messed around a little with writing a program that would set the date beyond 1999. Dates, as in directories, will show up in the form of 01-JAN-100, but it would probably be adequate. The program is in crude form now and I would like to refine it a bit more. I could then provide a copy to you or anyone else that needs one. } } Of course, if someone else has already tackled this issue, or if no one is still using a PDP-based system (hard to believe), then I can better spend my time on other tasks. Please contact me if you would be interested in such a program. } } } ---------------------- } Warren E. Straszheim
I cannot imagine why anyone would still use a PDP or LSI-11 system. Even the VAX has been discontinued. I can appreciate the cost arguments surrounding changing a system. Is there a way to keep a detector yet add a new pulse processor and a standard PC? Imagine what can be done with more than 60KW of memory?
I still have some LSI-11 boards here but even the dog won't fetch them anymore.
In the 70's and early 80's I worked on RT-11 and TSX. I had the source code for RT-11 on an RK-05 (wow....2.5MB on a 12" diameter platter) and later on an RL-01 and 02. Yes, the date is biased in octal. I changed the bias rather easily and did a rebuild. Same for RSX-11 (not as easy). If you can get the source, try a re-build and edit. The other option is to use the disk editor and locate the bias and change it. If I recall, the date is created by adding the set bias value, which as you point out, is not 4 digits worth.
Hum....so for a 2000 hour year, this works out to be $10 per hour. What a gift. And they probably want $30/hour of work in return.
No matter how you package it, all of this babble does not measure up to today's standards. Unless SEM, etc. is a obscure and diminutive endeavor, I simply do not understand the cost-benefit ratio. Maybe this is not an annual salary. OK. Is this in addition to an existing income stream? Geeze, I hope it is the latter. But it sounds like the position is on-site. So, the candidate gets a full time job at McDonald's as well?
All I can say is that I am glad and relieved that I do not have to work and try to survive in this type of environment. Welcome to H-2 visas.
gary g.
At 08:14 AM 12/31/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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